Search results for: Sakornrat Khongkhunthian

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Khongkhunthian</title> <meta name="description" content="Search results for: Sakornrat Khongkhunthian"> <meta name="keywords" content="Sakornrat Khongkhunthian"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Sakornrat Khongkhunthian"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Sakornrat Khongkhunthian</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Effects of Gelatin on Characteristics and Dental Pathogen Inhibition by Silver Nanoparticles Synthesized from Ascorbic Acid</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Siriporn%20Okonogi">Siriporn Okonogi</a>, <a href="https://publications.waset.org/abstracts/search?q=Temsiri%20%20Suwan"> Temsiri Suwan</a>, <a href="https://publications.waset.org/abstracts/search?q=Sakornrat%20%20Khongkhunthian"> Sakornrat Khongkhunthian</a>, <a href="https://publications.waset.org/abstracts/search?q=Jakkapan%20%20Sirithunyalug"> Jakkapan Sirithunyalug</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, silver nanoparticles (AgNPs) were prepared using ascorbic acid as a reducing agent and silver nitrate as a precursor. The effects of gelatin (G) on particle characteristics and dental pathogen inhibition were investigated. The spectra of AgNPs and G-AgNPs were compared using UV-Vis and Energy-dispersive X-ray (EDX) spectroscopy. The obtained AgNPs and G-AgNPs showed the maximum absorption at 410 and 430 nm, respectively, and EDX spectra of both systems confirmed Ag element. Scanning electron microscope showed that AgNPs and G-AgNPs were spherical in shape. Particles size, size distribution, and zeta potential were determined using dynamic light scattering approach. The size of AgNPs and G-AgNPs were 56 卤 2.4 and 67 卤 3.6 nm, respectively with a size distribution of 0.23 卤 0.03 and 0.19 卤 0.02, respectively. AgNPs and G-AgNPs exhibited negative zeta potential of 24.1 卤 2.7 mV and 32.7 卤 1.2 mV, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the obtained AgNPs and G-AgNPs against three strains of dental pathogenic bacteria; Streptococcus gordonii, Streptococcus mutans, and Staphylococcus aureus were determined using broth dilution method. AgNPs and G-AgNPs showed the strongest inhibition against S. gordonii with the MIC of 0.05 and 0.025 mg/mL, respectively and the MBC of 0.1 and 0.05 mg/mL, respectively. Cytotoxicity test of AgNPs and G-AgNPs on human breast cancer cells using MTT assay indicated that G-AgNPs (0.1 mg/mL) was significantly stronger toxic than AgNPs with the cell inhibition of 91.1 卤 5.4%. G-AgNPs showed significantly less aggregation after storage at room temperature for 90 days than G-AgNPs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antipathogenic%20activity" title="antipathogenic activity">antipathogenic activity</a>, <a href="https://publications.waset.org/abstracts/search?q=ascorbic%20acid" title=" ascorbic acid"> ascorbic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=stability" title=" stability"> stability</a> </p> <a href="https://publications.waset.org/abstracts/105937/effects-of-gelatin-on-characteristics-and-dental-pathogen-inhibition-by-silver-nanoparticles-synthesized-from-ascorbic-acid" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/105937.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">149</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Antibacterial Activity and Cytotoxicity of Silver Nanoparticles Synthesized by Moringa oleifera Extract as Reducing Agent</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Temsiri%20Suwan">Temsiri Suwan</a>, <a href="https://publications.waset.org/abstracts/search?q=Penpicha%20Wanachantararak"> Penpicha Wanachantararak</a>, <a href="https://publications.waset.org/abstracts/search?q=Sakornrat%20Khongkhunthian"> Sakornrat Khongkhunthian</a>, <a href="https://publications.waset.org/abstracts/search?q=Siriporn%20Okonogi"> Siriporn Okonogi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present study, silver nanoparticles (AgNPs) were synthesized by green synthesis approach using Moringa oleifera aqueous extract (ME) as a reducing agent and silver nitrate as a precursor. The obtained AgNPs were characterized using UV-Vis spectroscopy (UV-Vis), dynamic light scattering (DLS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffractometry (XRD). The results from UV-Vis revealed that the maximum absorption of AgNPs was at 430 nm and the EDX spectrum confirmed Ag element. The results from DLS indicated that the amount of ME played an important role in particle size, size distribution, and zeta potential of the obtained AgNPs. The smallest size (62.4 卤 1.8 nm) with narrow distribution (0.18 卤 0.02) of AgNPs was obtained after using 1% w/v of ME. This system gave high negative zeta potential of -36.5 卤 2.8 mV. SEM results indicated that the obtained AgNPs were spherical in shape. Antibacterial activity using dilution method revealed that the minimum inhibitory and minimum bactericidal concentrations of the obtained AgNPs against Streptococcus mutans were 0.025 and 0.1 mg/mL, respectively. Cytotoxicity test of AgNPs on adenocarcinomic human alveolar basal epithelial cells (A549) indicated that the particles impacted against A549 cells. The percentage of cell growth inhibition was 87.5 卤 3.6 % when only 0.1 mg/mL AgNPs was used. These results suggest that ME is the potential reducing agent for green synthesis of AgNPs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibacterial%20activity" title="antibacterial activity">antibacterial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=Moringa%20oleifera%20extract" title=" Moringa oleifera extract"> Moringa oleifera extract</a>, <a href="https://publications.waset.org/abstracts/search?q=reducing%20agent" title=" reducing agent"> reducing agent</a>, <a href="https://publications.waset.org/abstracts/search?q=silver%20nanoparticles" title=" silver nanoparticles"> silver nanoparticles</a> </p> <a href="https://publications.waset.org/abstracts/105935/antibacterial-activity-and-cytotoxicity-of-silver-nanoparticles-synthesized-by-moringa-oleifera-extract-as-reducing-agent" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/105935.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">108</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> 4-Allylpyrocatechol Loaded Polymeric Micelles for Solubility Enhancing and Effects on Streptococcus mutans Biofilms</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Siriporn%20Okonogi">Siriporn Okonogi</a>, <a href="https://publications.waset.org/abstracts/search?q=Pimpak%20Phumat"> Pimpak Phumat</a>, <a href="https://publications.waset.org/abstracts/search?q=Sakornrat%20Khongkhunthian"> Sakornrat Khongkhunthian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Piper betle has been extensively reported for various pharmacological effects including antimicrobial activity. 4-Allylpyrocatechol (AC) is a principle active compound found in P. betle. However, AC has a problem of solubility in water. The aims of the present study were to prepare AC loaded polymeric micelles for enhancing its water solubility and to evaluate its anti-biofilm activity against oral phathogenic bacteria. AC was loaded in polymeric micelles (PM) of Pluronic F127 by using thin film hydration method to obtain AC loaded PM (PMAC). The results revealed that AC in the form of PMAC possessed high water solubility. PMAC particles were characterized using a transmission electron microscope and photon correlation spectroscopy. Determination of entrapment efficiency (EE) and loading capacity (LC) of PMAC was done by using high-performance liquid chromatography. The highest EE (86.33 卤 14.27 %) and LC (19.25 卤 3.18 %) of PMAC were found when the weight ratio of polymer to AC was 4 to 1. At this ratio, the particles showed spherical in shape with the size of 38.83 卤 1.36 nm and polydispersity index of 0.28 卤 0.10. Zeta potential of the particles is negative with the value of 16.43 卤 0.55 mV. Crystal violet assay and confocal microscopy were applied to evaluate the effects of PMAC on Streptococcus mutans biofilms using chlorhexidine (CHX) as a positive control. PMAC contained 1.5 mg/mL AC could potentially inhibit (102.01 卤 9.18%) and significantly eradicate (85.05 卤 2.03 %) these biofilms (p < 0.05). Comparison with CHX, PMAC showed slightly similar biofilm inhibition but significantly stronger biofilm eradication (p < 0.05) than CHX. It is concluded that PMAC can enhance water solubility and anti-biofilm activity of AC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pluronic" title="pluronic">pluronic</a>, <a href="https://publications.waset.org/abstracts/search?q=polymeric%20micelles" title=" polymeric micelles"> polymeric micelles</a>, <a href="https://publications.waset.org/abstracts/search?q=solubility" title=" solubility"> solubility</a>, <a href="https://publications.waset.org/abstracts/search?q=4-allylpyrocathecol" title=" 4-allylpyrocathecol"> 4-allylpyrocathecol</a>, <a href="https://publications.waset.org/abstracts/search?q=Streptococcus%20mutans" title=" Streptococcus mutans"> Streptococcus mutans</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-biofilm" title=" anti-biofilm"> anti-biofilm</a> </p> <a href="https://publications.waset.org/abstracts/106535/4-allylpyrocatechol-loaded-polymeric-micelles-for-solubility-enhancing-and-effects-on-streptococcus-mutans-biofilms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/106535.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">144</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Development of 4-Allylpyrocatechol Loaded Self-Nanoemulsifying Drug Delivery System for Enhancing Water Solubility and Antibacterial Activity against Oral Pathogenic Bacteria </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pimpak%20Phumat">Pimpak Phumat</a>, <a href="https://publications.waset.org/abstracts/search?q=Sakornrat%20Khongkhunthian"> Sakornrat Khongkhunthian</a>, <a href="https://publications.waset.org/abstracts/search?q=Thomas%20Rades"> Thomas Rades</a>, <a href="https://publications.waset.org/abstracts/search?q=Anette%20M%C3%BCllertz"> Anette M眉llertz</a>, <a href="https://publications.waset.org/abstracts/search?q=Siriporn%20Okonogi"> Siriporn Okonogi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Self-nanoemulsifying drug delivery systems (SNEDDS) containing 4-allylpyrocatechol (AP) extracted from Piper betle were developed to enhance water solubility of AP by using modeling and design (MODDE) program. The amount of AP in each SNEDDS formulation was determined by using high-performance liquid chromatography. The formulation consisted of 20% Miglyol庐812N, 40 % Kolliphor庐RH40, 30 % Maisine庐35-1 and 10 % ethanol was found to be the best SNEDDS that provided the highest loading capacity of AP. (141.48卤15.64 mg/g SNEDDS). The system also showed miscibility with water. The particle shape and size of the AP-SNEDDS after dispersing in water was investigated by using a transmission electron microscope and photon correlation spectrophotometer, respectively. The results showed that they were a spherical shape, having a particle size of 34.27 卤 1.14 nm with a narrow size distribution of 0.17 卤 0.04. The particles showed negative zeta potential with a value of -21.66 卤 2.09 mV. Antibacterial activity of AP-SNEDDS containing 1.5 mg/mL of AP was investigated against Streptococcus intermedius. The effect of this system on S. intermedius cells was observed by a scanning electron microscope (SEM). The results from SEM revealed that the bacterial cells were obviously destroyed. Killing kinetic study of AP-SNEDDS was carried out. It was found that the killing rate of AP-SNEDDS against S. intermedius was dose-dependent and the bacterial reduction was 79.86 卤 0.45 % within 30 min. In comparison with chlorhexidine (CHX), AP-SNEDDS showed similar antibacterial effects against S. intermedius. It is concluded that SNEDDS is a potential system for enhancing water solubility of AP. The antibacterial study reveals that AP-SNEDDS can be a promising system to treat bacterial infection caused by S. intermedius. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=SNEDDS" title="SNEDDS">SNEDDS</a>, <a href="https://publications.waset.org/abstracts/search?q=4-allylpyrocathecol" title=" 4-allylpyrocathecol"> 4-allylpyrocathecol</a>, <a href="https://publications.waset.org/abstracts/search?q=solubility" title=" solubility"> solubility</a>, <a href="https://publications.waset.org/abstracts/search?q=antibacterial%20activity" title=" antibacterial activity"> antibacterial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=Streptococcus%20intermedius" title=" Streptococcus intermedius "> Streptococcus intermedius </a> </p> <a href="https://publications.waset.org/abstracts/106536/development-of-4-allylpyrocatechol-loaded-self-nanoemulsifying-drug-delivery-system-for-enhancing-water-solubility-and-antibacterial-activity-against-oral-pathogenic-bacteria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/106536.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">119</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" 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Search results for: Sakornrat Khongkhunthian