CINXE.COM

<!DOCTYPE html> <html lang="en" xmlns:fb="http://www.facebook.com/2008/fbml" class="wf-loading"> <head prefix="og: https://ogp.me/ns# fb: https://ogp.me/ns/fb# academia: https://ogp.me/ns/fb/academia#"> <meta charset="utf-8"> <meta name=viewport content="width=device-width, initial-scale=1"> <meta rel="search" type="application/opensearchdescription+xml" href="/open_search.xml" title="Academia.edu"> <title>Peroxidase Research Papers - Academia.edu</title>  <link href="//a.academia-assets.com/images/favicons/favicon-production.ico" rel="shortcut icon" type="image/vnd.microsoft.icon"> <link rel="apple-touch-icon" sizes="57x57" href="//a.academia-assets.com/images/favicons/apple-touch-icon-57x57.png"> <link rel="apple-touch-icon" sizes="60x60" href="//a.academia-assets.com/images/favicons/apple-touch-icon-60x60.png"> <link rel="apple-touch-icon" sizes="72x72" href="//a.academia-assets.com/images/favicons/apple-touch-icon-72x72.png"> <link rel="apple-touch-icon" sizes="76x76" href="//a.academia-assets.com/images/favicons/apple-touch-icon-76x76.png"> <link rel="apple-touch-icon" sizes="114x114" href="//a.academia-assets.com/images/favicons/apple-touch-icon-114x114.png"> <link rel="apple-touch-icon" sizes="120x120" href="//a.academia-assets.com/images/favicons/apple-touch-icon-120x120.png"> <link rel="apple-touch-icon" sizes="144x144" href="//a.academia-assets.com/images/favicons/apple-touch-icon-144x144.png"> <link rel="apple-touch-icon" sizes="152x152" href="//a.academia-assets.com/images/favicons/apple-touch-icon-152x152.png"> <link rel="apple-touch-icon" sizes="180x180" href="//a.academia-assets.com/images/favicons/apple-touch-icon-180x180.png"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-32x32.png" sizes="32x32"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-194x194.png" sizes="194x194"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-96x96.png" sizes="96x96"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/android-chrome-192x192.png" sizes="192x192"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-16x16.png" sizes="16x16"> <link rel="manifest" href="//a.academia-assets.com/images/favicons/manifest.json"> <meta name="msapplication-TileColor" content="#2b5797"> <meta name="msapplication-TileImage" content="//a.academia-assets.com/images/favicons/mstile-144x144.png"> <meta name="theme-color" content="#ffffff"> <script> window.performance && window.performance.measure && window.performance.measure("Time To First Byte", "requestStart", "responseStart"); </script> <script> (function() { if (!window.URLSearchParams || !window.history || !window.history.replaceState) { return; } var searchParams = new URLSearchParams(window.location.search); var paramsToDelete = [ 'fs', 'sm', 'swp', 'iid', 'nbs', 'rcc', // related content category 'rcpos', // related content carousel position 'rcpg', // related carousel page 'rchid', // related content hit id 'f_ri', // research interest id, for SEO tracking 'f_fri', // featured research interest, for SEO tracking (param key without value) 'f_rid', // from research interest directory for SEO tracking 'f_loswp', // from research interest pills on LOSWP sidebar for SEO tracking 'rhid', // referrring hit id ]; if (paramsToDelete.every((key) => searchParams.get(key) === null)) { return; } paramsToDelete.forEach((key) => { searchParams.delete(key); }); var cleanUrl = new URL(window.location.href); cleanUrl.search = searchParams.toString(); history.replaceState({}, document.title, cleanUrl); })(); </script> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "by_tag", 'action': "show_one", 'controller_action': 'by_tag#show_one', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script type="text/javascript"> window.sendUserTiming = function(timingName) { if (!(window.performance && window.performance.measure)) return; var entries = window.performance.getEntriesByName(timingName, "measure"); if (entries.length !== 1) return; var timingValue = Math.round(entries[0].duration); gtag('event', 'timing_complete', { name: timingName, value: timingValue, event_category: 'User-centric', }); }; window.sendUserTiming("Time To First Byte"); </script> <meta name="csrf-param" content="authenticity_token" /> <meta name="csrf-token" content="kaRz5c6I_9407tNswVLAZ_U2cigZCi7sjADNtx043hJ_BxEqhcq_uzciHtxTHyCqZtkk_EpN428dYfxgxmUfkg" /> <link href="/Documents/in/Peroxidase?after=50%2C19219747" rel="next" /><link crossorigin="" href="https://fonts.gstatic.com/" rel="preconnect" /><link href="https://fonts.googleapis.com/css2?family=DM+Sans:ital,opsz,wght@0,9..40,100..1000;1,9..40,100..1000&family=Gupter:wght@400;500;700&family=IBM+Plex+Mono:wght@300;400&family=Material+Symbols+Outlined:opsz,wght,FILL,GRAD@20,400,0,0&display=swap" rel="stylesheet" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/common-2b6f90dbd75f5941bc38f4ad716615f3ac449e7398313bb3bc225fba451cd9fa.css" /> <meta name="description" content="View Peroxidase Research Papers on Academia.edu for free." /> <meta name="google-site-verification" content="bKJMBZA7E43xhDOopFZkssMMkBRjvYERV-NaN4R6mrs" /> <script> var $controller_name = 'by_tag'; var $action_name = "show_one"; var $rails_env = 'production'; var $app_rev = 'dc2ad41da5d7ea682babd20f90650302fb0a3a36'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.Aedu = { hit_data: null }; window.Aedu.SiteStats = {"premium_universities_count":14016,"monthly_visitors":"99 million","monthly_visitor_count":99567017,"monthly_visitor_count_in_millions":99,"user_count":282994021,"paper_count":55203019,"paper_count_in_millions":55,"page_count":432000000,"page_count_in_millions":432,"pdf_count":16500000,"pdf_count_in_millions":16}; window.Aedu.serverRenderTime = new Date(1739805236000); window.Aedu.timeDifference = new Date().getTime() - 1739805236000; window.Aedu.isUsingCssV1 = false; window.Aedu.enableLocalization = true; window.Aedu.activateFullstory = false; window.Aedu.serviceAvailability = { status: {"attention_db":"on","bibliography_db":"on","contacts_db":"on","email_db":"on","indexability_db":"on","mentions_db":"on","news_db":"on","notifications_db":"on","offsite_mentions_db":"on","redshift":"on","redshift_exports_db":"on","related_works_db":"on","ring_db":"on","user_tests_db":"on"}, serviceEnabled: function(service) { return this.status[service] === "on"; }, readEnabled: function(service) { return this.serviceEnabled(service) || this.status[service] === "read_only"; }, }; window.Aedu.viewApmTrace = function() { // Check if x-apm-trace-id meta tag is set, and open the trace in APM // in a new window if it is. var apmTraceId = document.head.querySelector('meta[name="x-apm-trace-id"]'); if (apmTraceId) { var traceId = apmTraceId.content; // Use trace ID to construct URL, an example URL looks like: // https://app.datadoghq.com/apm/traces?query=trace_id%31298410148923562634 var apmUrl = 'https://app.datadoghq.com/apm/traces?query=trace_id%3A' + traceId; window.open(apmUrl, '_blank'); } }; </script>  <link href="https://fonts.googleapis.com/css?family=Roboto:100,100i,300,300i,400,400i,500,500i,700,700i,900,900i" rel="stylesheet"> <link rel="preload" href="//maxcdn.bootstrapcdn.com/font-awesome/4.3.0/css/font-awesome.min.css" as="style" onload="this.rel='stylesheet'"> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/libraries-a9675dcb01ec4ef6aa807ba772c7a5a00c1820d3ff661c1038a20f80d06bb4e4.css" /> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/academia-40698df34f913bd208bb70f09d2feb7c6286046250be17a4db35bba2c08b0e2f.css" /> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system_legacy-056a9113b9a0f5343d013b29ee1929d5a18be35fdcdceb616600b4db8bd20054.css" /> <script src="//a.academia-assets.com/assets/webpack_bundles/runtime-bundle-005434038af4252ca37c527588411a3d6a0eabb5f727fac83f8bbe7fd88d93bb.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/webpack_libraries_and_infrequently_changed.wjs-bundle-a22f75d8519394c21253dae46c8c5d60ad36ea68c7d494347ec64229d8c1cf85.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/core_webpack.wjs-bundle-5708a105dd66b4c7d0ef30b7c094b1048423f0042bd2a7b123f2d99ee3cf46d9.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/sentry.wjs-bundle-5fe03fddca915c8ba0f7edbe64c194308e8ce5abaed7bffe1255ff37549c4808.js"></script> <script> jade = window.jade || {}; jade.helpers = window.$h; jade._ = window._; </script>  <script id="tag-manager-head-root">(function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer_old','GTM-5G9JF7Z');</script>  <script> window.gptadslots = []; window.googletag = window.googletag || {}; window.googletag.cmd = window.googletag.cmd || []; </script> <script type="text/javascript"> // TODO(jacob): This should be defined, may be rare load order problem. // Checking if null is just a quick fix, will default to en if unset. // Better fix is to run this immedietely after I18n is set. if (window.I18n != null) { I18n.defaultLocale = "en"; I18n.locale = "en"; I18n.fallbacks = true; } </script> <link rel="canonical" href="https://www.academia.edu/Documents/in/Peroxidase" /> </head>   <body class='c-by_tag a-show_one logged_out u-bgColorWhite'>  <div id="fb-root"></div><script>window.fbAsyncInit = function() { FB.init({ appId: "2369844204", version: "v8.0", status: true, cookie: true, xfbml: true }); // Additional initialization code. if (window.InitFacebook) { // facebook.ts already loaded, set it up. window.InitFacebook(); } else { // Set a flag for facebook.ts to find when it loads. window.academiaAuthReadyFacebook = true; } };</script><script>window.fbAsyncLoad = function() { // Protection against double calling of this function if (window.FB) { return; } (function(d, s, id){ var js, fjs = d.getElementsByTagName(s)[0]; if (d.getElementById(id)) {return;} js = d.createElement(s); js.id = id; js.src = "//connect.facebook.net/en_US/sdk.js"; fjs.parentNode.insertBefore(js, fjs); }(document, 'script', 'facebook-jssdk')); } if (!window.defer_facebook) { // Autoload if not deferred window.fbAsyncLoad(); } else { // Defer loading by 5 seconds setTimeout(function() { window.fbAsyncLoad(); }, 5000); }</script> <div id="google-root"></div><script>window.loadGoogle = function() { if (window.InitGoogle) { // google.ts already loaded, set it up. window.InitGoogle("331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b"); } else { // Set a flag for google.ts to use when it loads. window.GoogleClientID = "331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b"; } };</script><script>window.googleAsyncLoad = function() { // Protection against double calling of this function (function(d) { var js; var id = 'google-jssdk'; var ref = d.getElementsByTagName('script')[0]; if (d.getElementById(id)) { return; } js = d.createElement('script'); js.id = id; js.async = true; js.onload = loadGoogle; js.src = "https://accounts.google.com/gsi/client" ref.parentNode.insertBefore(js, ref); }(document)); } if (!window.defer_google) { // Autoload if not deferred window.googleAsyncLoad(); } else { // Defer loading by 5 seconds setTimeout(function() { window.googleAsyncLoad(); }, 5000); }</script> <div id="tag-manager-body-root">  <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-5G9JF7Z" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript>   <script> window.addEventListener('load', function() { if (document.querySelector('input[name="commit"]')) { document.querySelector('input[name="commit"]').addEventListener('click', function() { gtag('event', 'click', { event_category: 'button', event_label: 'Log In' }) }) } }); </script> </div> <script>var _comscore = _comscore || []; _comscore.push({ c1: "2", c2: "26766707" }); (function() { var s = document.createElement("script"), el = document.getElementsByTagName("script")[0]; s.async = true; s.src = (document.location.protocol == "https:" ? "https://sb" : "http://b") + ".scorecardresearch.com/beacon.js"; el.parentNode.insertBefore(s, el); })();</script><img src="https://sb.scorecardresearch.com/p?c1=2&c2=26766707&cv=2.0&cj=1" style="position: absolute; visibility: hidden" /> <div id='react-modal'></div> <div class='DesignSystem'> <a class='u-showOnFocus' href='#site'> Skip to main content </a> </div> <div id="upgrade_ie_banner" style="display: none;"><p>Academia.edu no longer supports Internet Explorer.</p><p>To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to <a href="https://www.academia.edu/upgrade-browser">upgrade your browser</a>.</p></div><script>// Show this banner for all versions of IE if (!!window.MSInputMethodContext || /(MSIE)/.test(navigator.userAgent)) { document.getElementById('upgrade_ie_banner').style.display = 'block'; }</script> <div class="DesignSystem bootstrap ShrinkableNav no-sm no-md"><div class="navbar navbar-default main-header"><div class="container-wrapper" id="main-header-container"><div class="container"><div class="navbar-header"><div class="nav-left-wrapper u-mt0x"><div class="nav-logo"><a data-main-header-link-target="logo_home" href="https://www.academia.edu/"><img class="visible-xs-inline-block" style="height: 24px;" alt="Academia.edu" src="//a.academia-assets.com/images/academia-logo-redesign-2015-A.svg" width="24" height="24" /><img width="145.2" height="18" class="hidden-xs" style="height: 24px;" alt="Academia.edu" src="//a.academia-assets.com/images/academia-logo-redesign-2015.svg" /></a></div><div class="nav-search"><div class="SiteSearch-wrapper select2-no-default-pills"><form class="js-SiteSearch-form DesignSystem" action="https://www.academia.edu/search" accept-charset="UTF-8" method="get"><i class="SiteSearch-icon fa fa-search u-fw700 u-positionAbsolute u-tcGrayDark"></i><input class="js-SiteSearch-form-input SiteSearch-form-input form-control" data-main-header-click-target="search_input" name="q" placeholder="Search" type="text" value="" /></form></div></div></div><div class="nav-right-wrapper pull-right"><ul class="NavLinks js-main-nav list-unstyled"><li class="NavLinks-link"><a class="js-header-login-url Button Button--inverseGray Button--sm u-mb4x" id="nav_log_in" rel="nofollow" href="https://www.academia.edu/login">Log In</a></li><li class="NavLinks-link u-p0x"><a class="Button Button--inverseGray Button--sm u-mb4x" rel="nofollow" href="https://www.academia.edu/signup">Sign Up</a></li></ul><button class="hidden-lg hidden-md hidden-sm u-ml4x navbar-toggle collapsed" data-target=".js-mobile-header-links" data-toggle="collapse" type="button"><span class="icon-bar"></span><span class="icon-bar"></span><span class="icon-bar"></span></button></div></div><div class="collapse navbar-collapse js-mobile-header-links"><ul class="nav navbar-nav"><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/login">Log In</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/signup">Sign Up</a></li><li class="u-borderColorGrayLight u-borderBottom1 js-mobile-nav-expand-trigger"><a href="#">more&nbsp<span class="caret"></span></a></li><li><ul class="js-mobile-nav-expand-section nav navbar-nav u-m0x collapse"><li class="u-borderColorGrayLight u-borderBottom1"><a rel="false" href="https://www.academia.edu/about">About</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/press">Press</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="false" href="https://www.academia.edu/documents">Papers</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/terms">Terms</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/privacy">Privacy</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/copyright">Copyright</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/hiring"><i class="fa fa-briefcase"></i> We're Hiring!</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://support.academia.edu/hc/en-us"><i class="fa fa-question-circle"></i> Help Center</a></li><li class="js-mobile-nav-collapse-trigger u-borderColorGrayLight u-borderBottom1 dropup" style="display:none"><a href="#">less&nbsp<span class="caret"></span></a></li></ul></li></ul></div></div></div><script>(function(){ var $moreLink = $(".js-mobile-nav-expand-trigger"); var $lessLink = $(".js-mobile-nav-collapse-trigger"); var $section = $('.js-mobile-nav-expand-section'); $moreLink.click(function(ev){ ev.preventDefault(); $moreLink.hide(); $lessLink.show(); $section.collapse('show'); }); $lessLink.click(function(ev){ ev.preventDefault(); $moreLink.show(); $lessLink.hide(); $section.collapse('hide'); }); })() if ($a.is_logged_in() || false) { new Aedu.NavigationController({ el: '.js-main-nav', showHighlightedNotification: false }); } else { $(".js-header-login-url").attr("href", $a.loginUrlWithRedirect()); } Aedu.autocompleteSearch = new AutocompleteSearch({el: '.js-SiteSearch-form'});</script></div></div> <div id='site' class='fixed'> <div id="content" class="clearfix"> <script>document.addEventListener('DOMContentLoaded', function(){ var $dismissible = $(".dismissible_banner"); $dismissible.click(function(ev) { $dismissible.hide(); }); });</script> <div class="DesignSystem" style="margin-top:-40px"><div class="PageHeader"><div class="container"><div class="row"><style type="text/css">.sor-abstract { display: -webkit-box; overflow: hidden; text-overflow: ellipsis; -webkit-line-clamp: 3; -webkit-box-orient: vertical; }</style><div class="col-xs-12 clearfix"><div class="u-floatLeft"><h1 class="PageHeader-title u-m0x u-fs30">Peroxidase</h1><div class="u-tcGrayDark">3,590 Followers</div><div class="u-tcGrayDark u-mt2x">Recent papers in <b>Peroxidase</b></div></div></div></div></div></div><div class="TabbedNavigation"><div class="container"><div class="row"><div class="col-xs-12 clearfix"><ul class="nav u-m0x u-p0x list-inline u-displayFlex"><li class="active"><a href="https://www.academia.edu/Documents/in/Peroxidase">Top Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Peroxidase/MostCited">Most Cited Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Peroxidase/MostDownloaded">Most Downloaded Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Peroxidase/MostRecent">Newest Papers</a></li><li><a class="" href="https://www.academia.edu/People/Peroxidase">People</a></li></ul></div><style type="text/css">ul.nav{flex-direction:row}@media(max-width: 567px){ul.nav{flex-direction:column}.TabbedNavigation li{max-width:100%}.TabbedNavigation li.active{background-color:var(--background-grey, #dddde2)}.TabbedNavigation li.active:before,.TabbedNavigation li.active:after{display:none}}</style></div></div></div><div class="container"><div class="row"><div class="col-xs-12"><div class="u-displayFlex"><div class="u-flexGrow1"><div class="works"><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_42022525" data-work_id="42022525" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/42022525/Detection_of_soluble_peroxidases_by_direct_plant_tissue_isoelectric_focusing">Detection of soluble peroxidases by direct plant tissue isoelectric focusing</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Rectangular sections of leaves. vacuum-infiltrated with distilled water or aaueous v solutions of potassium chloride, were applied directly onto the surface of polyacrylamide gels followed by isoelectric focusing. Soluble peroxidases... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_42022525" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Rectangular sections of leaves. vacuum-infiltrated with distilled water or aaueous v solutions of potassium chloride, were applied directly onto the surface of polyacrylamide gels followed by isoelectric focusing. Soluble peroxidases (free and ionically bound) were released from the tissue on isoelectric focusing with resultant characteristic zymograms for the different plant tissues.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/42022525" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1a50ffaf0d97da5fd766c706f41d4cf3" rel="nofollow" data-download="{"attachment_id":62148158,"asset_id":42022525,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/62148158/download_file?st=MTczOTgwNTIzNCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="20599849" href="https://independent.academia.edu/Jos%C3%A9RCastellano">José R N Castellano Castellano</a><script data-card-contents-for-user="20599849" type="text/json">{"id":20599849,"first_name":"José","last_name":"Castellano Castellano","domain_name":"independent","page_name":"JoséRCastellano","display_name":"José R N Castellano Castellano","profile_url":"https://independent.academia.edu/Jos%C3%A9RCastellano?f_ri=55175","photo":"https://0.academia-photos.com/20599849/13685028/14817773/s65_jos_r.castellano.jpg"}</script></span></span></li><li class="js-paper-rank-work_42022525 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="42022525"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 42022525, container: ".js-paper-rank-work_42022525", }); });</script></li><li class="js-percentile-work_42022525 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 42022525; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_42022525"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_42022525 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="42022525"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42022525; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42022525]").text(description); $(".js-view-count-work_42022525").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_42022525").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="42022525"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">5</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="72" rel="nofollow" href="https://www.academia.edu/Documents/in/Chemical_Engineering">Chemical Engineering</a>, <script data-card-contents-for-ri="72" type="text/json">{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="524" rel="nofollow" href="https://www.academia.edu/Documents/in/Analytical_Chemistry">Analytical Chemistry</a>, <script data-card-contents-for-ri="524" type="text/json">{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="371425" rel="nofollow" href="https://www.academia.edu/Documents/in/Electrophoresis">Electrophoresis</a><script data-card-contents-for-ri="371425" type="text/json">{"id":371425,"name":"Electrophoresis","url":"https://www.academia.edu/Documents/in/Electrophoresis?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=42022525]'), work: {"id":42022525,"title":"Detection of soluble peroxidases by direct plant tissue isoelectric focusing","created_at":"2020-02-20T00:24:14.110-08:00","url":"https://www.academia.edu/42022525/Detection_of_soluble_peroxidases_by_direct_plant_tissue_isoelectric_focusing?f_ri=55175","dom_id":"work_42022525","summary":"Rectangular sections of leaves. vacuum-infiltrated with distilled water or aaueous v solutions of potassium chloride, were applied directly onto the surface of polyacrylamide gels followed by isoelectric focusing. Soluble peroxidases (free and ionically bound) were released from the tissue on isoelectric focusing with resultant characteristic zymograms for the different plant tissues.","downloadable_attachments":[{"id":62148158,"asset_id":42022525,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":20599849,"first_name":"José","last_name":"Castellano Castellano","domain_name":"independent","page_name":"JoséRCastellano","display_name":"José R N Castellano Castellano","profile_url":"https://independent.academia.edu/Jos%C3%A9RCastellano?f_ri=55175","photo":"https://0.academia-photos.com/20599849/13685028/14817773/s65_jos_r.castellano.jpg"}],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=55175","nofollow":true},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":371425,"name":"Electrophoresis","url":"https://www.academia.edu/Documents/in/Electrophoresis?f_ri=55175","nofollow":true},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1854650" data-work_id="1854650" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1854650/Lipid_peroxidation_and_antioxidant_enzyme_activities_of_i_Euphorbia_millii_i_hyperhydric_shoots">Lipid peroxidation and antioxidant enzyme activities of< i> Euphorbia millii</i> hyperhydric shoots</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A large number of micropropagated Euphorbia millii shoots from temporary immersion bioreactor showed thick broad leaves that were translucent, wrinkled and/or curled and brittle, symptoms of hyperhydricity. The environment inside... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_1854650" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A large number of micropropagated Euphorbia millii shoots from temporary immersion bioreactor showed thick broad leaves that were translucent, wrinkled and/or curled and brittle, symptoms of hyperhydricity. The environment inside bioreactor normally used in plant micropropagation is characterised by high relative humidity, poor gaseous exchange between the internal atmosphere of the bioreactor and its surrounding environment, and the accumulation of ethylene, conditions that may induce physiological disorders. A comparison of hyperhydric shoots (HS) with normal plants shows marked increase in malondialdehyde (MDA) content in HS plants. MDA, a decomposition product of polyunsaturated fatty acids hydroperoxides, has been utilized very often as a suitable biomarker for lipid peroxidation, which is an effect of oxidative damage. This hypothesis is also confirmed by the higher lipoxygenase (LOX) activity in HS plants. The potential role of antioxidant enzymes in protecting hyperhydric shoots from oxidative injury was examined by analyzing enzyme activities and isozyme profiles of hyperhydric and non-hyperhydric leaves of E. millii. Superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity were significantly higher in hyperhydric tissue as compared to non-hyperhydric normal leaf tissue. After native polyacrylamide gel electrophoresis (PAGE) analysis, seven SOD isoenzymes were detected and the increase in SOD activity observed in hyperhydric tissue seemed to be mainly due to Mn-SOD and Cu/Zn-SOD. The activity of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) was proportionally increased in HS tissue compared to normal leaves indicating a crucial role in eliminating toxic H 2 O 2 from plant cells. The depletion of GSH and total glutathione in spite of higher GR activities observed in HS tissue indicates that mechanism of antioxidant defense was by enhanced oxidation of GSH to GSSG by DHAR yielding ascorbate (AA). The antioxidant metabolism has been shown to be important in determining the ability of plants to survive in hyperhydric stress and the up regulation of these enzymes would help to reduce the build up of ROS.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/1854650" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8a3c5545120b8f36586362ded001beae" rel="nofollow" data-download="{"attachment_id":31298620,"asset_id":1854650,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/31298620/download_file?st=MTczOTgwNTIzNCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="2301895" href="https://kfs.academia.edu/YaserDewir">Yaser Dewir</a><script data-card-contents-for-user="2301895" type="text/json">{"id":2301895,"first_name":"Yaser","last_name":"Dewir","domain_name":"kfs","page_name":"YaserDewir","display_name":"Yaser Dewir","profile_url":"https://kfs.academia.edu/YaserDewir?f_ri=55175","photo":"https://0.academia-photos.com/2301895/1723322/2064764/s65_yaser.dewir.jpg"}</script></span></span></li><li class="js-paper-rank-work_1854650 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="1854650"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 1854650, container: ".js-paper-rank-work_1854650", }); });</script></li><li class="js-percentile-work_1854650 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 1854650; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_1854650"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_1854650 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="1854650"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 1854650; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=1854650]").text(description); $(".js-view-count-work_1854650").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_1854650").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="1854650"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">60</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="654" rel="nofollow" href="https://www.academia.edu/Documents/in/Andrology">Andrology</a>, <script data-card-contents-for-ri="654" type="text/json">{"id":654,"name":"Andrology","url":"https://www.academia.edu/Documents/in/Andrology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1290" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3243" rel="nofollow" href="https://www.academia.edu/Documents/in/Nonparametric_Statistics">Nonparametric Statistics</a>, <script data-card-contents-for-ri="3243" type="text/json">{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6791" rel="nofollow" href="https://www.academia.edu/Documents/in/Aging">Aging</a><script data-card-contents-for-ri="6791" type="text/json">{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=1854650]'), work: {"id":1854650,"title":"Lipid peroxidation and antioxidant enzyme activities of\u003c i\u003e Euphorbia millii\u003c/i\u003e hyperhydric shoots","created_at":"2012-08-13T14:07:52.969-07:00","url":"https://www.academia.edu/1854650/Lipid_peroxidation_and_antioxidant_enzyme_activities_of_i_Euphorbia_millii_i_hyperhydric_shoots?f_ri=55175","dom_id":"work_1854650","summary":"A large number of micropropagated Euphorbia millii shoots from temporary immersion bioreactor showed thick broad leaves that were translucent, wrinkled and/or curled and brittle, symptoms of hyperhydricity. The environment inside bioreactor normally used in plant micropropagation is characterised by high relative humidity, poor gaseous exchange between the internal atmosphere of the bioreactor and its surrounding environment, and the accumulation of ethylene, conditions that may induce physiological disorders. A comparison of hyperhydric shoots (HS) with normal plants shows marked increase in malondialdehyde (MDA) content in HS plants. MDA, a decomposition product of polyunsaturated fatty acids hydroperoxides, has been utilized very often as a suitable biomarker for lipid peroxidation, which is an effect of oxidative damage. This hypothesis is also confirmed by the higher lipoxygenase (LOX) activity in HS plants. The potential role of antioxidant enzymes in protecting hyperhydric shoots from oxidative injury was examined by analyzing enzyme activities and isozyme profiles of hyperhydric and non-hyperhydric leaves of E. millii. Superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity were significantly higher in hyperhydric tissue as compared to non-hyperhydric normal leaf tissue. After native polyacrylamide gel electrophoresis (PAGE) analysis, seven SOD isoenzymes were detected and the increase in SOD activity observed in hyperhydric tissue seemed to be mainly due to Mn-SOD and Cu/Zn-SOD. The activity of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) was proportionally increased in HS tissue compared to normal leaves indicating a crucial role in eliminating toxic H 2 O 2 from plant cells. The depletion of GSH and total glutathione in spite of higher GR activities observed in HS tissue indicates that mechanism of antioxidant defense was by enhanced oxidation of GSH to GSSG by DHAR yielding ascorbate (AA). The antioxidant metabolism has been shown to be important in determining the ability of plants to survive in hyperhydric stress and the up regulation of these enzymes would help to reduce the build up of ROS.","downloadable_attachments":[{"id":31298620,"asset_id":1854650,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":2301895,"first_name":"Yaser","last_name":"Dewir","domain_name":"kfs","page_name":"YaserDewir","display_name":"Yaser Dewir","profile_url":"https://kfs.academia.edu/YaserDewir?f_ri=55175","photo":"https://0.academia-photos.com/2301895/1723322/2064764/s65_yaser.dewir.jpg"}],"research_interests":[{"id":654,"name":"Andrology","url":"https://www.academia.edu/Documents/in/Andrology?f_ri=55175","nofollow":true},{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=55175","nofollow":true},{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=55175","nofollow":true},{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging?f_ri=55175","nofollow":true},{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=55175"},{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=55175"},{"id":44022,"name":"Free Radical","url":"https://www.academia.edu/Documents/in/Free_Radical?f_ri=55175"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175"},{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=55175"},{"id":55163,"name":"Enzymes","url":"https://www.academia.edu/Documents/in/Enzymes?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=55175"},{"id":69056,"name":"Testosterone","url":"https://www.academia.edu/Documents/in/Testosterone?f_ri=55175"},{"id":75318,"name":"Smoking","url":"https://www.academia.edu/Documents/in/Smoking?f_ri=55175"},{"id":82978,"name":"Reactive Oxygen Species","url":"https://www.academia.edu/Documents/in/Reactive_Oxygen_Species?f_ri=55175"},{"id":90514,"name":"Cholesterol","url":"https://www.academia.edu/Documents/in/Cholesterol?f_ri=55175"},{"id":91863,"name":"Alcoholism","url":"https://www.academia.edu/Documents/in/Alcoholism?f_ri=55175"},{"id":97766,"name":"Vascular dementia","url":"https://www.academia.edu/Documents/in/Vascular_dementia?f_ri=55175"},{"id":114835,"name":"Experimental Botany","url":"https://www.academia.edu/Documents/in/Experimental_Botany?f_ri=55175"},{"id":118450,"name":"Glutathione","url":"https://www.academia.edu/Documents/in/Glutathione?f_ri=55175"},{"id":123230,"name":"Regression Analysis","url":"https://www.academia.edu/Documents/in/Regression_Analysis?f_ri=55175"},{"id":125564,"name":"Statistical Significance","url":"https://www.academia.edu/Documents/in/Statistical_Significance?f_ri=55175"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=55175"},{"id":141395,"name":"Glutathione Peroxidase","url":"https://www.academia.edu/Documents/in/Glutathione_Peroxidase?f_ri=55175"},{"id":149539,"name":"Polychlorinated Biphenyls","url":"https://www.academia.edu/Documents/in/Polychlorinated_Biphenyls?f_ri=55175"},{"id":158884,"name":"Urinary Tract Infections","url":"https://www.academia.edu/Documents/in/Urinary_Tract_Infections?f_ri=55175"},{"id":178928,"name":"Male Infertility","url":"https://www.academia.edu/Documents/in/Male_Infertility?f_ri=55175"},{"id":203521,"name":"tESTIS","url":"https://www.academia.edu/Documents/in/tESTIS?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":227299,"name":"Triglycerides","url":"https://www.academia.edu/Documents/in/Triglycerides?f_ri=55175"},{"id":230744,"name":"Relative Humidity","url":"https://www.academia.edu/Documents/in/Relative_Humidity?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":233372,"name":"Lipid peroxidation","url":"https://www.academia.edu/Documents/in/Lipid_peroxidation?f_ri=55175"},{"id":242478,"name":"Polychlorinated Biphenyl","url":"https://www.academia.edu/Documents/in/Polychlorinated_Biphenyl?f_ri=55175"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=55175"},{"id":246560,"name":"High Pressure Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Pressure_Liquid_Chromatography?f_ri=55175"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=55175"},{"id":295257,"name":"Antioxidant enzyme","url":"https://www.academia.edu/Documents/in/Antioxidant_enzyme?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=55175"},{"id":427871,"name":"Oxidative Damage","url":"https://www.academia.edu/Documents/in/Oxidative_Damage?f_ri=55175"},{"id":583568,"name":"Enzyme activity","url":"https://www.academia.edu/Documents/in/Enzyme_activity?f_ri=55175"},{"id":587528,"name":"Bacterial infections","url":"https://www.academia.edu/Documents/in/Bacterial_infections?f_ri=55175"},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=55175"},{"id":915951,"name":"Type 2 Diabetes Mellitus","url":"https://www.academia.edu/Documents/in/Type_2_Diabetes_Mellitus?f_ri=55175"},{"id":963536,"name":"Sperm Count","url":"https://www.academia.edu/Documents/in/Sperm_Count?f_ri=55175"},{"id":975992,"name":"Polyacrylamide Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Polyacrylamide_Gel_Electrophoresis?f_ri=55175"},{"id":982534,"name":"Erythrocytes","url":"https://www.academia.edu/Documents/in/Erythrocytes?f_ri=55175"},{"id":1011463,"name":"Glutathione Reductase","url":"https://www.academia.edu/Documents/in/Glutathione_Reductase?f_ri=55175"},{"id":1120234,"name":"Alzheimer Disease","url":"https://www.academia.edu/Documents/in/Alzheimer_Disease?f_ri=55175"},{"id":1130425,"name":"Luteinizing Hormone","url":"https://www.academia.edu/Documents/in/Luteinizing_Hormone?f_ri=55175"},{"id":1310139,"name":"Follicle stimulating hormone","url":"https://www.academia.edu/Documents/in/Follicle_stimulating_hormone?f_ri=55175"},{"id":1446339,"name":"Malondialdehyde","url":"https://www.academia.edu/Documents/in/Malondialdehyde?f_ri=55175"},{"id":1546570,"name":"Xanthine Oxidase","url":"https://www.academia.edu/Documents/in/Xanthine_Oxidase?f_ri=55175"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=55175"},{"id":1819399,"name":"Case Control Studies","url":"https://www.academia.edu/Documents/in/Case_Control_Studies?f_ri=55175"},{"id":1954742,"name":"Pentose Phosphate Pathway","url":"https://www.academia.edu/Documents/in/Pentose_Phosphate_Pathway?f_ri=55175"},{"id":1991090,"name":"Neurochemical","url":"https://www.academia.edu/Documents/in/Neurochemical?f_ri=55175"},{"id":2278598,"name":"Ascorbate Peroxidase","url":"https://www.academia.edu/Documents/in/Ascorbate_Peroxidase?f_ri=55175"},{"id":2573890,"name":"Body Weight Gain","url":"https://www.academia.edu/Documents/in/Body_Weight_Gain?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22569938" data-work_id="22569938" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22569938/Effects_of_Oxidants_on_Properties_of_Fluorescent_Calcium_Indicators">Effects of Oxidants on Properties of Fluorescent Calcium Indicators</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">to know the susceptibility of fluorescent probes to oxi-An increasing number of studies use calcium-sensi-dants. This important issue is being addressed by the tive fluorescent dyes to address the relationship be-present study, which... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_22569938" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">to know the susceptibility of fluorescent probes to oxi-An increasing number of studies use calcium-sensi-dants. This important issue is being addressed by the tive fluorescent dyes to address the relationship be-present study, which evaluates the effects of several tween elevated levels of intracellular calcium and freeoxidant-generating systems on the spectral properties radical-mediated damage in a variety of pathophysioof such commonly used probes as Fluo-3 and Fura-2.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22569938" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7b232076a4627508e95a1067fe5ea407" rel="nofollow" data-download="{"attachment_id":43179282,"asset_id":22569938,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43179282/download_file?st=MTczOTgwNTIzNCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="44119461" href="https://independent.academia.edu/NSarvazyan">N. Sarvazyan</a><script data-card-contents-for-user="44119461" type="text/json">{"id":44119461,"first_name":"N.","last_name":"Sarvazyan","domain_name":"independent","page_name":"NSarvazyan","display_name":"N. Sarvazyan","profile_url":"https://independent.academia.edu/NSarvazyan?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_22569938 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="22569938"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 22569938, container: ".js-paper-rank-work_22569938", }); });</script></li><li class="js-percentile-work_22569938 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 22569938; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_22569938"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_22569938 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="22569938"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 22569938; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=22569938]").text(description); $(".js-view-count-work_22569938").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_22569938").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="22569938"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2425" rel="nofollow" href="https://www.academia.edu/Documents/in/Free_Radicals">Free Radicals</a>, <script data-card-contents-for-ri="2425" type="text/json">{"id":2425,"name":"Free Radicals","url":"https://www.academia.edu/Documents/in/Free_Radicals?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14292" rel="nofollow" href="https://www.academia.edu/Documents/in/Oxidative_Stress">Oxidative Stress</a>, <script data-card-contents-for-ri="14292" type="text/json">{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="18529" rel="nofollow" href="https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents">Fluorescent Dyes and Reagents</a><script data-card-contents-for-ri="18529" type="text/json">{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22569938]'), work: {"id":22569938,"title":"Effects of Oxidants on Properties of Fluorescent Calcium Indicators","created_at":"2016-02-28T19:28:44.221-08:00","url":"https://www.academia.edu/22569938/Effects_of_Oxidants_on_Properties_of_Fluorescent_Calcium_Indicators?f_ri=55175","dom_id":"work_22569938","summary":"to know the susceptibility of fluorescent probes to oxi-An increasing number of studies use calcium-sensi-dants. This important issue is being addressed by the tive fluorescent dyes to address the relationship be-present study, which evaluates the effects of several tween elevated levels of intracellular calcium and freeoxidant-generating systems on the spectral properties radical-mediated damage in a variety of pathophysioof such commonly used probes as Fluo-3 and Fura-2.","downloadable_attachments":[{"id":43179282,"asset_id":22569938,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":44119461,"first_name":"N.","last_name":"Sarvazyan","domain_name":"independent","page_name":"NSarvazyan","display_name":"N. Sarvazyan","profile_url":"https://independent.academia.edu/NSarvazyan?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2425,"name":"Free Radicals","url":"https://www.academia.edu/Documents/in/Free_Radicals?f_ri=55175","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=55175","nofollow":true},{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=55175","nofollow":true},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=55175","nofollow":true},{"id":44022,"name":"Free Radical","url":"https://www.academia.edu/Documents/in/Free_Radical?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":172678,"name":"Artifacts","url":"https://www.academia.edu/Documents/in/Artifacts?f_ri=55175"},{"id":730649,"name":"Spectral Properties","url":"https://www.academia.edu/Documents/in/Spectral_Properties?f_ri=55175"},{"id":1352490,"name":"Oxidants","url":"https://www.academia.edu/Documents/in/Oxidants?f_ri=55175"},{"id":1599862,"name":"Fura","url":"https://www.academia.edu/Documents/in/Fura?f_ri=55175"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=55175"},{"id":1782947,"name":"aniline Compounds","url":"https://www.academia.edu/Documents/in/aniline_Compounds?f_ri=55175"},{"id":1823382,"name":"Intracellular Calcium","url":"https://www.academia.edu/Documents/in/Intracellular_Calcium?f_ri=55175"},{"id":1956581,"name":"Dynamic Range","url":"https://www.academia.edu/Documents/in/Dynamic_Range?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16155154" data-work_id="16155154" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16155154/Oxidative_stress_induced_by_cadmium_in_Nicotiana_tabacum_L_effects_on_growth_parameters_oxidative_damage_and_antioxidant_responses_in_different_plant_parts">Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Tobacco (Nicotiana tabacum L.) is a tolerant species that accumulates cadmium. We studied the effect of Cd (0, 10, 25, 50, 100 lM) on growth parameters, chlorophyll and proline contents, enzymatic antioxidative response and lipid... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16155154" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Tobacco (Nicotiana tabacum L.) is a tolerant species that accumulates cadmium. We studied the effect of Cd (0, 10, 25, 50, 100 lM) on growth parameters, chlorophyll and proline contents, enzymatic antioxidative response and lipid peroxidation of tobacco plants grown in hydroponic culture for 11 days to clarify the strategy of plant response against oxidative stress caused by this heavy metal. Cadmium accumulated more in roots than in shoots. Plant growth was not significantly affected by the cadmium concentrations used. Young leaves were more affected, showing visible chlorosis and a significant decrease in chlorophyll content at high Cd concentrations. Dry weight of both leaves and roots increased indicating a lower capacity for roots to absorb water. An increase in malondialdehyde levels was observed, indicating that lipid peroxidation occurred as a result of ROS formation. The activity of guaiacol peroxidase in leaves increased, indicating that it was very important in the scavenging of H 2 O 2 , while superoxide dismutase activity only increased in old leaves. Ascorbate peroxidase showed constant activity levels in tobacco leaves, suggesting that the ascorbate-glutathione pathway was less important as a defense mechanism.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16155154" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1e3ba3d562ab30f8665bfdc7e29ecae2" rel="nofollow" data-download="{"attachment_id":42664779,"asset_id":16155154,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42664779/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35266970" href="https://independent.academia.edu/AmarilisVarennes">Amarilis Varennes</a><script data-card-contents-for-user="35266970" type="text/json">{"id":35266970,"first_name":"Amarilis","last_name":"Varennes","domain_name":"independent","page_name":"AmarilisVarennes","display_name":"Amarilis Varennes","profile_url":"https://independent.academia.edu/AmarilisVarennes?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_16155154 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16155154"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16155154, container: ".js-paper-rank-work_16155154", }); });</script></li><li class="js-percentile-work_16155154 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16155154; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_16155154"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16155154 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16155154"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16155154; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16155154]").text(description); $(".js-view-count-work_16155154").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16155154").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16155154"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9109" rel="nofollow" href="https://www.academia.edu/Documents/in/Tobacco">Tobacco</a>, <script data-card-contents-for-ri="9109" type="text/json">{"id":9109,"name":"Tobacco","url":"https://www.academia.edu/Documents/in/Tobacco?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="111050" rel="nofollow" href="https://www.academia.edu/Documents/in/Heavy_Metal">Heavy Metal</a><script data-card-contents-for-ri="111050" type="text/json">{"id":111050,"name":"Heavy Metal","url":"https://www.academia.edu/Documents/in/Heavy_Metal?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16155154]'), work: {"id":16155154,"title":"Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts","created_at":"2015-09-25T00:25:50.752-07:00","url":"https://www.academia.edu/16155154/Oxidative_stress_induced_by_cadmium_in_Nicotiana_tabacum_L_effects_on_growth_parameters_oxidative_damage_and_antioxidant_responses_in_different_plant_parts?f_ri=55175","dom_id":"work_16155154","summary":"Tobacco (Nicotiana tabacum L.) is a tolerant species that accumulates cadmium. We studied the effect of Cd (0, 10, 25, 50, 100 lM) on growth parameters, chlorophyll and proline contents, enzymatic antioxidative response and lipid peroxidation of tobacco plants grown in hydroponic culture for 11 days to clarify the strategy of plant response against oxidative stress caused by this heavy metal. Cadmium accumulated more in roots than in shoots. Plant growth was not significantly affected by the cadmium concentrations used. Young leaves were more affected, showing visible chlorosis and a significant decrease in chlorophyll content at high Cd concentrations. Dry weight of both leaves and roots increased indicating a lower capacity for roots to absorb water. An increase in malondialdehyde levels was observed, indicating that lipid peroxidation occurred as a result of ROS formation. The activity of guaiacol peroxidase in leaves increased, indicating that it was very important in the scavenging of H 2 O 2 , while superoxide dismutase activity only increased in old leaves. Ascorbate peroxidase showed constant activity levels in tobacco leaves, suggesting that the ascorbate-glutathione pathway was less important as a defense mechanism.","downloadable_attachments":[{"id":42664779,"asset_id":16155154,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35266970,"first_name":"Amarilis","last_name":"Varennes","domain_name":"independent","page_name":"AmarilisVarennes","display_name":"Amarilis Varennes","profile_url":"https://independent.academia.edu/AmarilisVarennes?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true},{"id":9109,"name":"Tobacco","url":"https://www.academia.edu/Documents/in/Tobacco?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":111050,"name":"Heavy Metal","url":"https://www.academia.edu/Documents/in/Heavy_Metal?f_ri=55175","nofollow":true},{"id":118450,"name":"Glutathione","url":"https://www.academia.edu/Documents/in/Glutathione?f_ri=55175"},{"id":127006,"name":"Plant growth","url":"https://www.academia.edu/Documents/in/Plant_growth?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":295257,"name":"Antioxidant enzyme","url":"https://www.academia.edu/Documents/in/Antioxidant_enzyme?f_ri=55175"},{"id":317826,"name":"Nicotiana Tabacum","url":"https://www.academia.edu/Documents/in/Nicotiana_Tabacum?f_ri=55175"},{"id":427871,"name":"Oxidative Damage","url":"https://www.academia.edu/Documents/in/Oxidative_Damage?f_ri=55175"},{"id":1446339,"name":"Malondialdehyde","url":"https://www.academia.edu/Documents/in/Malondialdehyde?f_ri=55175"},{"id":1675937,"name":"chlorophyll Content","url":"https://www.academia.edu/Documents/in/chlorophyll_Content?f_ri=55175"},{"id":2278598,"name":"Ascorbate Peroxidase","url":"https://www.academia.edu/Documents/in/Ascorbate_Peroxidase?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_77154142" data-work_id="77154142" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/77154142/Benefits_of_membrane_electrodes_in_the_electrochemistry_of_metalloproteins_mediated_catalysis_of_Paracoccus_pantotrophus_cytochrome_c_peroxidase_by_horse_cytochrome_c_a_case_study">Benefits of membrane electrodes in the electrochemistry of metalloproteins: mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by horse cytochrome c: a case study</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A comparative study of direct and mediated electrochemistry of metalloproteins in bulk and membraneentrapped solutions is presented. This work reports the first electrochemical study of the electron transfer between a bacterial cytochrome... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_77154142" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A comparative study of direct and mediated electrochemistry of metalloproteins in bulk and membraneentrapped solutions is presented. This work reports the first electrochemical study of the electron transfer between a bacterial cytochrome c peroxidase and horse heart cytochrome c. The mediated catalysis of the peroxidase was analysed both using the membrane electrode configuration and with all proteins in solution. An apparent Michaelis constant of 66 ± 4 and 42 ± 5 lM was determined at pH 7.0 and 0 M NaCl for membrane and bulk solutions, respectively. The data revealed that maximum activity occurs at 50 mM NaCl, pH 7.0, with intermolecular rate constants of (4.4 ± 0.5) 9 10 6 and (1.0 ± 0.5) 9 10 6 M -1 s -1 for membrane-entrapped and bulk solutions, respectively. The influence of parameters such as pH or ionic strength on the mediated catalytic activity was analysed using this approach, drawing attention to the fact that careful analysis of the results is needed to ensure that no artefacts are introduced by the use of the membrane configuration and/or promoters, and therefore the dependence truly reflects the influence of these parameters on the (mediated) catalysis. From the pH dependence, a pK of 7.5 was estimated for the mediated enzymatic catalysis. Membrane electrode Á Mediated catalysis Á Bacterial cytochrome c peroxidase Á Horse cytochrome c Abbreviations AUME Gold membrane electrode BCCP Bacterial cytochrome c peroxidase ME Membrane electrode</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/77154142" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1a1ce154f412aee2741301e3db26384e" rel="nofollow" data-download="{"attachment_id":84618105,"asset_id":77154142,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/84618105/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="96787851" href="https://independent.academia.edu/JoseMoura23">Jose Moura</a><script data-card-contents-for-user="96787851" type="text/json">{"id":96787851,"first_name":"Jose","last_name":"Moura","domain_name":"independent","page_name":"JoseMoura23","display_name":"Jose Moura","profile_url":"https://independent.academia.edu/JoseMoura23?f_ri=55175","photo":"https://0.academia-photos.com/96787851/21119626/20590788/s65_jose.moura.jpg"}</script></span></span></li><li class="js-paper-rank-work_77154142 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="77154142"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 77154142, container: ".js-paper-rank-work_77154142", }); });</script></li><li class="js-percentile-work_77154142 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77154142; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_77154142"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_77154142 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="77154142"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77154142; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77154142]").text(description); $(".js-view-count-work_77154142").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_77154142").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="77154142"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="530" rel="nofollow" href="https://www.academia.edu/Documents/in/Inorganic_Chemistry">Inorganic Chemistry</a>, <script data-card-contents-for-ri="530" type="text/json">{"id":530,"name":"Inorganic Chemistry","url":"https://www.academia.edu/Documents/in/Inorganic_Chemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4748" rel="nofollow" href="https://www.academia.edu/Documents/in/Electrochemistry">Electrochemistry</a>, <script data-card-contents-for-ri="4748" type="text/json">{"id":4748,"name":"Electrochemistry","url":"https://www.academia.edu/Documents/in/Electrochemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4749" rel="nofollow" href="https://www.academia.edu/Documents/in/Catalysis">Catalysis</a>, <script data-card-contents-for-ri="4749" type="text/json">{"id":4749,"name":"Catalysis","url":"https://www.academia.edu/Documents/in/Catalysis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=77154142]'), work: {"id":77154142,"title":"Benefits of membrane electrodes in the electrochemistry of metalloproteins: mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by horse cytochrome c: a case study","created_at":"2022-04-21T01:24:54.778-07:00","url":"https://www.academia.edu/77154142/Benefits_of_membrane_electrodes_in_the_electrochemistry_of_metalloproteins_mediated_catalysis_of_Paracoccus_pantotrophus_cytochrome_c_peroxidase_by_horse_cytochrome_c_a_case_study?f_ri=55175","dom_id":"work_77154142","summary":"A comparative study of direct and mediated electrochemistry of metalloproteins in bulk and membraneentrapped solutions is presented. This work reports the first electrochemical study of the electron transfer between a bacterial cytochrome c peroxidase and horse heart cytochrome c. The mediated catalysis of the peroxidase was analysed both using the membrane electrode configuration and with all proteins in solution. An apparent Michaelis constant of 66 ± 4 and 42 ± 5 lM was determined at pH 7.0 and 0 M NaCl for membrane and bulk solutions, respectively. The data revealed that maximum activity occurs at 50 mM NaCl, pH 7.0, with intermolecular rate constants of (4.4 ± 0.5) 9 10 6 and (1.0 ± 0.5) 9 10 6 M -1 s -1 for membrane-entrapped and bulk solutions, respectively. The influence of parameters such as pH or ionic strength on the mediated catalytic activity was analysed using this approach, drawing attention to the fact that careful analysis of the results is needed to ensure that no artefacts are introduced by the use of the membrane configuration and/or promoters, and therefore the dependence truly reflects the influence of these parameters on the (mediated) catalysis. From the pH dependence, a pK of 7.5 was estimated for the mediated enzymatic catalysis. Membrane electrode Á Mediated catalysis Á Bacterial cytochrome c peroxidase Á Horse cytochrome c Abbreviations AUME Gold membrane electrode BCCP Bacterial cytochrome c peroxidase ME Membrane electrode","downloadable_attachments":[{"id":84618105,"asset_id":77154142,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":96787851,"first_name":"Jose","last_name":"Moura","domain_name":"independent","page_name":"JoseMoura23","display_name":"Jose Moura","profile_url":"https://independent.academia.edu/JoseMoura23?f_ri=55175","photo":"https://0.academia-photos.com/96787851/21119626/20590788/s65_jose.moura.jpg"}],"research_interests":[{"id":530,"name":"Inorganic Chemistry","url":"https://www.academia.edu/Documents/in/Inorganic_Chemistry?f_ri=55175","nofollow":true},{"id":4748,"name":"Electrochemistry","url":"https://www.academia.edu/Documents/in/Electrochemistry?f_ri=55175","nofollow":true},{"id":4749,"name":"Catalysis","url":"https://www.academia.edu/Documents/in/Catalysis?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":73865,"name":"Potentiometry","url":"https://www.academia.edu/Documents/in/Potentiometry?f_ri=55175"},{"id":83359,"name":"Electron Transfer","url":"https://www.academia.edu/Documents/in/Electron_Transfer?f_ri=55175"},{"id":96047,"name":"Case Study","url":"https://www.academia.edu/Documents/in/Case_Study?f_ri=55175"},{"id":116476,"name":"Cytochrome C","url":"https://www.academia.edu/Documents/in/Cytochrome_C?f_ri=55175"},{"id":168196,"name":"Horses","url":"https://www.academia.edu/Documents/in/Horses?f_ri=55175"},{"id":186094,"name":"Electron Transport","url":"https://www.academia.edu/Documents/in/Electron_Transport?f_ri=55175"},{"id":234366,"name":"Metalloproteins","url":"https://www.academia.edu/Documents/in/Metalloproteins?f_ri=55175"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide?f_ri=55175"},{"id":392828,"name":"Myocardium","url":"https://www.academia.edu/Documents/in/Myocardium?f_ri=55175"},{"id":846015,"name":"Electrodes","url":"https://www.academia.edu/Documents/in/Electrodes?f_ri=55175"},{"id":970774,"name":"Ionic Strength","url":"https://www.academia.edu/Documents/in/Ionic_Strength?f_ri=55175"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=55175"},{"id":1277798,"name":"Catalytic Activity","url":"https://www.academia.edu/Documents/in/Catalytic_Activity?f_ri=55175"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=55175"},{"id":3430039,"name":"Rate Constant","url":"https://www.academia.edu/Documents/in/Rate_Constant?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_70388547" data-work_id="70388547" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/70388547/Decolourisation_of_Remazol_Brilliant_Blue_R_via_a_novel_Bjerkandera_sp_strain">Decolourisation of Remazol Brilliant Blue R via a novel Bjerkandera sp. strain</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A novel strain of Bjerkandera sp. (B33/3), with particularly high decolourisation activities upon Poly R-478 and Remazol Brilliant Blue R (RBBR) dyes, was isolated. The role of the ligninolytic extracellular enzymes produced by this... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_70388547" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A novel strain of Bjerkandera sp. (B33/3), with particularly high decolourisation activities upon Poly R-478 and Remazol Brilliant Blue R (RBBR) dyes, was isolated. The role of the ligninolytic extracellular enzymes produced by this strain on decolourisation of RBBR was studied in some depth. The basis of decolourisation is an enzyme-mediated process, in which the main enzyme responsible is a recently described peroxidase with capacity for oxidation of manganese, as well as veratryl alcohol and 2,6-dimethoxyphenol in a manganese-independent reaction.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/70388547" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d8ffd4e74b6b7ef9004b4f1ab58886e4" rel="nofollow" data-download="{"attachment_id":80213174,"asset_id":70388547,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/80213174/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="210131786" href="https://independent.academia.edu/PatriciaMoreira106">Patricia Moreira</a><script data-card-contents-for-user="210131786" type="text/json">{"id":210131786,"first_name":"Patricia","last_name":"Moreira","domain_name":"independent","page_name":"PatriciaMoreira106","display_name":"Patricia Moreira","profile_url":"https://independent.academia.edu/PatriciaMoreira106?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_70388547 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="70388547"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 70388547, container: ".js-paper-rank-work_70388547", }); });</script></li><li class="js-percentile-work_70388547 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 70388547; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_70388547"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_70388547 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="70388547"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 70388547; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=70388547]").text(description); $(".js-view-count-work_70388547").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_70388547").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="70388547"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" rel="nofollow" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="923" rel="nofollow" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5398" rel="nofollow" href="https://www.academia.edu/Documents/in/Biotechnology">Biotechnology</a><script data-card-contents-for-ri="5398" type="text/json">{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=70388547]'), work: {"id":70388547,"title":"Decolourisation of Remazol Brilliant Blue R via a novel Bjerkandera sp. strain","created_at":"2022-02-04T01:22:49.100-08:00","url":"https://www.academia.edu/70388547/Decolourisation_of_Remazol_Brilliant_Blue_R_via_a_novel_Bjerkandera_sp_strain?f_ri=55175","dom_id":"work_70388547","summary":"A novel strain of Bjerkandera sp. (B33/3), with particularly high decolourisation activities upon Poly R-478 and Remazol Brilliant Blue R (RBBR) dyes, was isolated. The role of the ligninolytic extracellular enzymes produced by this strain on decolourisation of RBBR was studied in some depth. The basis of decolourisation is an enzyme-mediated process, in which the main enzyme responsible is a recently described peroxidase with capacity for oxidation of manganese, as well as veratryl alcohol and 2,6-dimethoxyphenol in a manganese-independent reaction.","downloadable_attachments":[{"id":80213174,"asset_id":70388547,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":210131786,"first_name":"Patricia","last_name":"Moreira","domain_name":"independent","page_name":"PatriciaMoreira106","display_name":"Patricia Moreira","profile_url":"https://independent.academia.edu/PatriciaMoreira106?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=55175","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true},{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology?f_ri=55175","nofollow":true},{"id":30763,"name":"Dyes","url":"https://www.academia.edu/Documents/in/Dyes?f_ri=55175"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":76714,"name":"Color","url":"https://www.academia.edu/Documents/in/Color?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":326386,"name":"Anthraquinones","url":"https://www.academia.edu/Documents/in/Anthraquinones?f_ri=55175"},{"id":1746898,"name":"BASIDIOMYCOTA","url":"https://www.academia.edu/Documents/in/BASIDIOMYCOTA?f_ri=55175"},{"id":2839441,"name":"Remazol Brilliant Blue R","url":"https://www.academia.edu/Documents/in/Remazol_Brilliant_Blue_R?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_64942877" data-work_id="64942877" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/64942877/The_effects_of_the_Pringle_maneuver_on_the_pancreas_can_octreotide_be_protective">The effects of the Pringle maneuver on the pancreas: can octreotide be protective?</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The aim of this study was to evaluate the negative effects of the Pringle maneuver on pancreatic tissue with respect to the time of performing the maneuver. Moreover, the efficacy of octreotide therapy on pancreatic changes at the time of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_64942877" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The aim of this study was to evaluate the negative effects of the Pringle maneuver on pancreatic tissue with respect to the time of performing the maneuver. Moreover, the efficacy of octreotide therapy on pancreatic changes at the time of the Pringle maneuver was assessed. Fifty male Wistar Albino rats were randomized into 5 groups. The groups were formed as follows: Group A: sham operation, Group B: Pringle maneuver for 30 min plus octreotide (PM30-OCT), Group C: Pringle maneuver for 60 min plus octreotide (PM60-OCT) and Group D: Pringle maneuver for 30 min plus 0.9 % saline solution (PM30-SS), Pringle maneuver for 60 min plus 0.9 % saline solution (PM60-SS, Group E). Blood samples for the evaluation of both amylase and lipase levels were taken via the portal vein. Levels of glutathione, glutathione reductase, catalase, myeloperoxidase, nitric oxide, xanthine oxidase, malondialdehyde, tumour necrosis factor alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) were assessed in the ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/64942877" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d1b9e96627d698cc5fb24095d681e4fc" rel="nofollow" data-download="{"attachment_id":76744399,"asset_id":64942877,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/76744399/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33758211" href="https://independent.academia.edu/CigdemYenisey">Cigdem Yenisey</a><script data-card-contents-for-user="33758211" type="text/json">{"id":33758211,"first_name":"Cigdem","last_name":"Yenisey","domain_name":"independent","page_name":"CigdemYenisey","display_name":"Cigdem Yenisey","profile_url":"https://independent.academia.edu/CigdemYenisey?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_64942877 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="64942877"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 64942877, container: ".js-paper-rank-work_64942877", }); });</script></li><li class="js-percentile-work_64942877 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 64942877; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_64942877"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_64942877 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="64942877"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 64942877; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=64942877]").text(description); $(".js-view-count-work_64942877").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_64942877").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="64942877"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="26327" rel="nofollow" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="92788" rel="nofollow" href="https://www.academia.edu/Documents/in/Pancreas">Pancreas</a>, <script data-card-contents-for-ri="92788" type="text/json">{"id":92788,"name":"Pancreas","url":"https://www.academia.edu/Documents/in/Pancreas?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="93922" rel="nofollow" href="https://www.academia.edu/Documents/in/Nitric_oxide">Nitric oxide</a><script data-card-contents-for-ri="93922" type="text/json">{"id":93922,"name":"Nitric oxide","url":"https://www.academia.edu/Documents/in/Nitric_oxide?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=64942877]'), work: {"id":64942877,"title":"The effects of the Pringle maneuver on the pancreas: can octreotide be protective?","created_at":"2021-12-18T00:57:43.630-08:00","url":"https://www.academia.edu/64942877/The_effects_of_the_Pringle_maneuver_on_the_pancreas_can_octreotide_be_protective?f_ri=55175","dom_id":"work_64942877","summary":"The aim of this study was to evaluate the negative effects of the Pringle maneuver on pancreatic tissue with respect to the time of performing the maneuver. Moreover, the efficacy of octreotide therapy on pancreatic changes at the time of the Pringle maneuver was assessed. Fifty male Wistar Albino rats were randomized into 5 groups. The groups were formed as follows: Group A: sham operation, Group B: Pringle maneuver for 30 min plus octreotide (PM30-OCT), Group C: Pringle maneuver for 60 min plus octreotide (PM60-OCT) and Group D: Pringle maneuver for 30 min plus 0.9 % saline solution (PM30-SS), Pringle maneuver for 60 min plus 0.9 % saline solution (PM60-SS, Group E). Blood samples for the evaluation of both amylase and lipase levels were taken via the portal vein. Levels of glutathione, glutathione reductase, catalase, myeloperoxidase, nitric oxide, xanthine oxidase, malondialdehyde, tumour necrosis factor alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) were assessed in the ...","downloadable_attachments":[{"id":76744399,"asset_id":64942877,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33758211,"first_name":"Cigdem","last_name":"Yenisey","domain_name":"independent","page_name":"CigdemYenisey","display_name":"Cigdem Yenisey","profile_url":"https://independent.academia.edu/CigdemYenisey?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":92788,"name":"Pancreas","url":"https://www.academia.edu/Documents/in/Pancreas?f_ri=55175","nofollow":true},{"id":93922,"name":"Nitric oxide","url":"https://www.academia.edu/Documents/in/Nitric_oxide?f_ri=55175","nofollow":true},{"id":103298,"name":"Blood sampling","url":"https://www.academia.edu/Documents/in/Blood_sampling?f_ri=55175"},{"id":118450,"name":"Glutathione","url":"https://www.academia.edu/Documents/in/Glutathione?f_ri=55175"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=55175"},{"id":189774,"name":"Animal Experimentation","url":"https://www.academia.edu/Documents/in/Animal_Experimentation?f_ri=55175"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=55175"},{"id":382481,"name":"Edema","url":"https://www.academia.edu/Documents/in/Edema?f_ri=55175"},{"id":1011463,"name":"Glutathione Reductase","url":"https://www.academia.edu/Documents/in/Glutathione_Reductase?f_ri=55175"},{"id":1209754,"name":"Lipase","url":"https://www.academia.edu/Documents/in/Lipase?f_ri=55175"},{"id":1401310,"name":"Myeloperoxidase","url":"https://www.academia.edu/Documents/in/Myeloperoxidase?f_ri=55175"},{"id":1446339,"name":"Malondialdehyde","url":"https://www.academia.edu/Documents/in/Malondialdehyde?f_ri=55175"},{"id":1663252,"name":"Portal vein","url":"https://www.academia.edu/Documents/in/Portal_vein?f_ri=55175"},{"id":2624997,"name":"Octreotide","url":"https://www.academia.edu/Documents/in/Octreotide?f_ri=55175"},{"id":2699033,"name":"Albino Rat","url":"https://www.academia.edu/Documents/in/Albino_Rat?f_ri=55175"},{"id":3029115,"name":"Hepatectomy","url":"https://www.academia.edu/Documents/in/Hepatectomy?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_48481360" data-work_id="48481360" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48481360/Activation_of_the_cytochrome_c_peroxidase_of_Pseudomonas_aeruginosa_The_role_of_a_heme_linked_protein_loop_A_mutagenesis_studies">Activation of the cytochrome c peroxidase of Pseudomonas aeruginosa. The role of a heme-linked protein loop: A mutagenesis studies</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Mutagenesis studies have been used to investigate the role of a heme ligand containing protein loop (67-79) in the activation of diheme peroxidases. Two mutant forms of the cytochrome c peroxidase of Pseudomonas aeruginosa have been... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48481360" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Mutagenesis studies have been used to investigate the role of a heme ligand containing protein loop (67-79) in the activation of diheme peroxidases. Two mutant forms of the cytochrome c peroxidase of Pseudomonas aeruginosa have been produced. One mutant (loop mutant) is devoid of the protein loop and the other (H71G) contains a non-ligating Gly at the normal histidine ligand site. Spectroscopic data show that in both mutants the distal histidine ligand of the peroxidatic heme in the un-activated enzyme is lost or is exchangeable. The un-activated H71G and loop mutants show, respectively, 75% and 10% of turnover activity of the wild-type enzyme in the activated form, in the presence of hydrogen peroxide and the physiological electron donor cytochrome c 551. Both mutant proteins show the presence of constitutive reactivity with peroxide in the normally inactive, fully oxidised, form of the enzyme and produce a radical intermediate. The radical product of the constitutive peroxide reaction appears to be located at different sites in the two mutant proteins. These results show that the loss of the histidine ligand from the peroxidatic heme is, in itself, sufficient to produce peroxidatic activity by providing a peroxide binding site and that the formation of radical intermediates is very sensitive to changes in protein structure. Overall, these data are consistent with a major role for the protein loop 67-79 in the activation of di-heme peroxidases and suggest a ''charge hopping'' mechanism may be operative in the process of intra-molecular electron transfer.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48481360" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d72f8e8bdd5211a7e776278046615cf6" rel="nofollow" data-download="{"attachment_id":67070050,"asset_id":48481360,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/67070050/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39495394" href="https://independent.academia.edu/sboycheva">svetlana boycheva</a><script data-card-contents-for-user="39495394" type="text/json">{"id":39495394,"first_name":"svetlana","last_name":"boycheva","domain_name":"independent","page_name":"sboycheva","display_name":"svetlana boycheva","profile_url":"https://independent.academia.edu/sboycheva?f_ri=55175","photo":"https://0.academia-photos.com/39495394/127695452/117081747/s65_svetlana.boycheva.png"}</script></span></span></li><li class="js-paper-rank-work_48481360 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48481360"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48481360, container: ".js-paper-rank-work_48481360", }); });</script></li><li class="js-percentile-work_48481360 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 48481360; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_48481360"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_48481360 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="48481360"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 48481360; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=48481360]").text(description); $(".js-view-count-work_48481360").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_48481360").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="48481360"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="530" rel="nofollow" href="https://www.academia.edu/Documents/in/Inorganic_Chemistry">Inorganic Chemistry</a>, <script data-card-contents-for-ri="530" type="text/json">{"id":530,"name":"Inorganic Chemistry","url":"https://www.academia.edu/Documents/in/Inorganic_Chemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="52770" rel="nofollow" href="https://www.academia.edu/Documents/in/Molecular_Electronics">Molecular Electronics</a>, <script data-card-contents-for-ri="52770" type="text/json">{"id":52770,"name":"Molecular Electronics","url":"https://www.academia.edu/Documents/in/Molecular_Electronics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="67401" rel="nofollow" href="https://www.academia.edu/Documents/in/Mutagenesis">Mutagenesis</a><script data-card-contents-for-ri="67401" type="text/json">{"id":67401,"name":"Mutagenesis","url":"https://www.academia.edu/Documents/in/Mutagenesis?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48481360]'), work: {"id":48481360,"title":"Activation of the cytochrome c peroxidase of Pseudomonas aeruginosa. The role of a heme-linked protein loop: A mutagenesis studies","created_at":"2021-05-04T20:10:28.250-07:00","url":"https://www.academia.edu/48481360/Activation_of_the_cytochrome_c_peroxidase_of_Pseudomonas_aeruginosa_The_role_of_a_heme_linked_protein_loop_A_mutagenesis_studies?f_ri=55175","dom_id":"work_48481360","summary":"Mutagenesis studies have been used to investigate the role of a heme ligand containing protein loop (67-79) in the activation of diheme peroxidases. Two mutant forms of the cytochrome c peroxidase of Pseudomonas aeruginosa have been produced. One mutant (loop mutant) is devoid of the protein loop and the other (H71G) contains a non-ligating Gly at the normal histidine ligand site. Spectroscopic data show that in both mutants the distal histidine ligand of the peroxidatic heme in the un-activated enzyme is lost or is exchangeable. The un-activated H71G and loop mutants show, respectively, 75% and 10% of turnover activity of the wild-type enzyme in the activated form, in the presence of hydrogen peroxide and the physiological electron donor cytochrome c 551. Both mutant proteins show the presence of constitutive reactivity with peroxide in the normally inactive, fully oxidised, form of the enzyme and produce a radical intermediate. The radical product of the constitutive peroxide reaction appears to be located at different sites in the two mutant proteins. These results show that the loss of the histidine ligand from the peroxidatic heme is, in itself, sufficient to produce peroxidatic activity by providing a peroxide binding site and that the formation of radical intermediates is very sensitive to changes in protein structure. Overall, these data are consistent with a major role for the protein loop 67-79 in the activation of di-heme peroxidases and suggest a ''charge hopping'' mechanism may be operative in the process of intra-molecular electron transfer.","downloadable_attachments":[{"id":67070050,"asset_id":48481360,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39495394,"first_name":"svetlana","last_name":"boycheva","domain_name":"independent","page_name":"sboycheva","display_name":"svetlana boycheva","profile_url":"https://independent.academia.edu/sboycheva?f_ri=55175","photo":"https://0.academia-photos.com/39495394/127695452/117081747/s65_svetlana.boycheva.png"}],"research_interests":[{"id":530,"name":"Inorganic Chemistry","url":"https://www.academia.edu/Documents/in/Inorganic_Chemistry?f_ri=55175","nofollow":true},{"id":52770,"name":"Molecular Electronics","url":"https://www.academia.edu/Documents/in/Molecular_Electronics?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":67401,"name":"Mutagenesis","url":"https://www.academia.edu/Documents/in/Mutagenesis?f_ri=55175","nofollow":true},{"id":116476,"name":"Cytochrome C","url":"https://www.academia.edu/Documents/in/Cytochrome_C?f_ri=55175"},{"id":166367,"name":"Protein structure","url":"https://www.academia.edu/Documents/in/Protein_structure?f_ri=55175"},{"id":204375,"name":"Heme","url":"https://www.academia.edu/Documents/in/Heme?f_ri=55175"},{"id":225499,"name":"Pseudomonas aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_aeruginosa?f_ri=55175"},{"id":322549,"name":"Activity","url":"https://www.academia.edu/Documents/in/Activity?f_ri=55175"},{"id":372917,"name":"Protein Secondary Structure Prediction","url":"https://www.academia.edu/Documents/in/Protein_Secondary_Structure_Prediction?f_ri=55175"},{"id":636395,"name":"Pseudomonas Aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_Aeruginosa-1?f_ri=55175"},{"id":712543,"name":"Glycine","url":"https://www.academia.edu/Documents/in/Glycine?f_ri=55175"},{"id":773664,"name":"Inorganic Biochemistry","url":"https://www.academia.edu/Documents/in/Inorganic_Biochemistry?f_ri=55175"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins?f_ri=55175"},{"id":1217930,"name":"Site-directed Mutagenesis","url":"https://www.academia.edu/Documents/in/Site-directed_Mutagenesis?f_ri=55175"},{"id":1222191,"name":"Ligands","url":"https://www.academia.edu/Documents/in/Ligands?f_ri=55175"},{"id":1242506,"name":"Binding Site","url":"https://www.academia.edu/Documents/in/Binding_Site?f_ri=55175"},{"id":1365896,"name":"Histidine","url":"https://www.academia.edu/Documents/in/Histidine?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1361405" data-work_id="1361405" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1361405/Crystal_structure_of_a_novel_human_peroxidase_enzyme_at_2_0_%C3%85_resolution">Crystal structure of a novel human peroxidase enzyme at 2.0 Å resolution</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/1361405" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="12a4b2c8208aff1eff8d114bf989e0de" rel="nofollow" data-download="{"attachment_id":51011476,"asset_id":1361405,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/51011476/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="512584" href="https://hanyang.academia.edu/SeongEonRyu">Seong Eon Ryu</a><script data-card-contents-for-user="512584" type="text/json">{"id":512584,"first_name":"Seong Eon","last_name":"Ryu","domain_name":"hanyang","page_name":"SeongEonRyu","display_name":"Seong Eon Ryu","profile_url":"https://hanyang.academia.edu/SeongEonRyu?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_1361405 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="1361405"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 1361405, container: ".js-paper-rank-work_1361405", }); });</script></li><li class="js-percentile-work_1361405 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 1361405; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_1361405"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_1361405 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="1361405"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 1361405; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=1361405]").text(description); $(".js-view-count-work_1361405").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_1361405").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="1361405"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3971" rel="nofollow" href="https://www.academia.edu/Documents/in/Protein_Folding">Protein Folding</a>, <script data-card-contents-for-ri="3971" type="text/json">{"id":3971,"name":"Protein Folding","url":"https://www.academia.edu/Documents/in/Protein_Folding?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="33441" rel="nofollow" href="https://www.academia.edu/Documents/in/Macromolecular_X-Ray_Crystallography">Macromolecular X-Ray Crystallography</a>, <script data-card-contents-for-ri="33441" type="text/json">{"id":33441,"name":"Macromolecular X-Ray Crystallography","url":"https://www.academia.edu/Documents/in/Macromolecular_X-Ray_Crystallography?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="38831" rel="nofollow" href="https://www.academia.edu/Documents/in/Signal_Transduction">Signal Transduction</a>, <script data-card-contents-for-ri="38831" type="text/json">{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="50630" rel="nofollow" href="https://www.academia.edu/Documents/in/Crystal_structure">Crystal structure</a><script data-card-contents-for-ri="50630" type="text/json">{"id":50630,"name":"Crystal structure","url":"https://www.academia.edu/Documents/in/Crystal_structure?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=1361405]'), work: {"id":1361405,"title":"Crystal structure of a novel human peroxidase enzyme at 2.0 Å resolution","created_at":"2012-02-08T15:10:53.483-08:00","url":"https://www.academia.edu/1361405/Crystal_structure_of_a_novel_human_peroxidase_enzyme_at_2_0_%C3%85_resolution?f_ri=55175","dom_id":"work_1361405","summary":null,"downloadable_attachments":[{"id":51011476,"asset_id":1361405,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":512584,"first_name":"Seong Eon","last_name":"Ryu","domain_name":"hanyang","page_name":"SeongEonRyu","display_name":"Seong Eon Ryu","profile_url":"https://hanyang.academia.edu/SeongEonRyu?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3971,"name":"Protein Folding","url":"https://www.academia.edu/Documents/in/Protein_Folding?f_ri=55175","nofollow":true},{"id":33441,"name":"Macromolecular X-Ray Crystallography","url":"https://www.academia.edu/Documents/in/Macromolecular_X-Ray_Crystallography?f_ri=55175","nofollow":true},{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction?f_ri=55175","nofollow":true},{"id":50630,"name":"Crystal structure","url":"https://www.academia.edu/Documents/in/Crystal_structure?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":653665,"name":"Protein Conformation","url":"https://www.academia.edu/Documents/in/Protein_Conformation?f_ri=55175"},{"id":1809037,"name":"Dimerization","url":"https://www.academia.edu/Documents/in/Dimerization?f_ri=55175"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_30175029 coauthored" data-work_id="30175029" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/30175029/Two_subtypes_of_Churg_Strauss_syndrome_with_neuropathy_the_roles_of_eosinophils_and_ANCA">Two subtypes of Churg–Strauss syndrome with neuropathy: the roles of eosinophils and ANCA</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The aim of this study was to clarify the differences in the pathogenesis of neuropathy between myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)-positive and -negative patients with Churg-Strauss syndrome (CSS). Eight... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_30175029" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The aim of this study was to clarify the differences in the pathogenesis of neuropathy between myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)-positive and -negative patients with Churg-Strauss syndrome (CSS). Eight MPO-ANCA-positive and 14 MPO-ANCA-negative patients were included. In addition to the standard histology, nerve biopsies were examined, employing immunohistochemistry for eosinophil major basic protein and electron microscopy. The groups did not differ significantly in clinical profiles, including the peak disability score and number of blood eosinophils. In nerve biopsies, necrotizing vasculitis was found in 63% (5/8) of the ANCA-positive and 21% (3/14) of the ANCAnegative patients. Fibrinoid necrosis of vessel walls was noted in 4 ANCA-positive patients (50%), and in one ANCA-negative patient (p = 0039). In contrast, a large number of eosinophilic infiltrations in the epineurium was shown in 36% (5/14) of the ANCA-negative patients, with no eosinophilic infiltrations shown in ANCA-positive patients. In 3 ANCA-negative patients, endoneurial eosinophils were seen where focal axonal loss and capillary dilatation were occasionally noted. There may be 2 pathogenetic mechanisms of neuropathy with CSS: ANCA-related vascular fibrinoid necrosis, and a toxic eosinophilic effect on nerve fibers which is independent of ANCA. Therapy targeting activated eosinophils may be a possible treatment for intractable neuropathy of CSS.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/30175029" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="24ac9e9c60fc5dd6361505866399f0be" rel="nofollow" data-download="{"attachment_id":50632867,"asset_id":30175029,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50632867/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="57363141" href="https://independent.academia.edu/NobuyukiOka">Nobuyuki Oka</a><script data-card-contents-for-user="57363141" type="text/json">{"id":57363141,"first_name":"Nobuyuki","last_name":"Oka","domain_name":"independent","page_name":"NobuyukiOka","display_name":"Nobuyuki Oka","profile_url":"https://independent.academia.edu/NobuyukiOka?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-30175029">+1</span><div class="hidden js-additional-users-30175029"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/KazuoShigematsu">Kazuo Shigematsu</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-30175029'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-30175029').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_30175029 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="30175029"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 30175029, container: ".js-paper-rank-work_30175029", }); });</script></li><li class="js-percentile-work_30175029 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 30175029; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_30175029"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_30175029 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="30175029"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 30175029; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=30175029]").text(description); $(".js-view-count-work_30175029").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_30175029").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="30175029"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2184" rel="nofollow" href="https://www.academia.edu/Documents/in/Electron_Microscopy">Electron Microscopy</a>, <script data-card-contents-for-ri="2184" type="text/json">{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37826" rel="nofollow" href="https://www.academia.edu/Documents/in/Biopsy">Biopsy</a>, <script data-card-contents-for-ri="37826" type="text/json">{"id":37826,"name":"Biopsy","url":"https://www.academia.edu/Documents/in/Biopsy?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=30175029]'), work: {"id":30175029,"title":"Two subtypes of Churg–Strauss syndrome with neuropathy: the roles of eosinophils and ANCA","created_at":"2016-11-30T03:58:21.895-08:00","url":"https://www.academia.edu/30175029/Two_subtypes_of_Churg_Strauss_syndrome_with_neuropathy_the_roles_of_eosinophils_and_ANCA?f_ri=55175","dom_id":"work_30175029","summary":"The aim of this study was to clarify the differences in the pathogenesis of neuropathy between myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)-positive and -negative patients with Churg-Strauss syndrome (CSS). Eight MPO-ANCA-positive and 14 MPO-ANCA-negative patients were included. In addition to the standard histology, nerve biopsies were examined, employing immunohistochemistry for eosinophil major basic protein and electron microscopy. The groups did not differ significantly in clinical profiles, including the peak disability score and number of blood eosinophils. In nerve biopsies, necrotizing vasculitis was found in 63% (5/8) of the ANCA-positive and 21% (3/14) of the ANCAnegative patients. Fibrinoid necrosis of vessel walls was noted in 4 ANCA-positive patients (50%), and in one ANCA-negative patient (p = 0039). In contrast, a large number of eosinophilic infiltrations in the epineurium was shown in 36% (5/14) of the ANCA-negative patients, with no eosinophilic infiltrations shown in ANCA-positive patients. In 3 ANCA-negative patients, endoneurial eosinophils were seen where focal axonal loss and capillary dilatation were occasionally noted. There may be 2 pathogenetic mechanisms of neuropathy with CSS: ANCA-related vascular fibrinoid necrosis, and a toxic eosinophilic effect on nerve fibers which is independent of ANCA. Therapy targeting activated eosinophils may be a possible treatment for intractable neuropathy of CSS.","downloadable_attachments":[{"id":50632867,"asset_id":30175029,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":57363141,"first_name":"Nobuyuki","last_name":"Oka","domain_name":"independent","page_name":"NobuyukiOka","display_name":"Nobuyuki Oka","profile_url":"https://independent.academia.edu/NobuyukiOka?f_ri=55175","photo":"/images/s65_no_pic.png"},{"id":38736913,"first_name":"Kazuo","last_name":"Shigematsu","domain_name":"independent","page_name":"KazuoShigematsu","display_name":"Kazuo Shigematsu","profile_url":"https://independent.academia.edu/KazuoShigematsu?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=55175","nofollow":true},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=55175","nofollow":true},{"id":37826,"name":"Biopsy","url":"https://www.academia.edu/Documents/in/Biopsy?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=55175"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=55175"},{"id":371516,"name":"Eosinophils","url":"https://www.academia.edu/Documents/in/Eosinophils?f_ri=55175"},{"id":448603,"name":"Necrosis","url":"https://www.academia.edu/Documents/in/Necrosis?f_ri=55175"},{"id":469105,"name":"Retrospective Studies","url":"https://www.academia.edu/Documents/in/Retrospective_Studies?f_ri=55175"},{"id":677382,"name":"Churg Strauss Syndrome","url":"https://www.academia.edu/Documents/in/Churg_Strauss_Syndrome?f_ri=55175"},{"id":1401310,"name":"Myeloperoxidase","url":"https://www.academia.edu/Documents/in/Myeloperoxidase?f_ri=55175"},{"id":1423078,"name":"Nervous System Diseases","url":"https://www.academia.edu/Documents/in/Nervous_System_Diseases?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_8821825" data-work_id="8821825" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/8821825/One_lung_ventilation_For_how_long">One-lung ventilation: For how long</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest">Objective: Lung injury induced by one-lung ventilation is rare, but it is a condition that may result in high mortality. This study evaluates the effects of one-lung ventilation and occlusion time on collapsed and contralateral lungs.</div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/8821825" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="846f164e4c62855f25fba7d642395c13" rel="nofollow" data-download="{"attachment_id":47990639,"asset_id":8821825,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47990639/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="19161182" href="https://independent.academia.edu/AAlver">Ahmet Alver</a><script data-card-contents-for-user="19161182" type="text/json">{"id":19161182,"first_name":"Ahmet","last_name":"Alver","domain_name":"independent","page_name":"AAlver","display_name":"Ahmet Alver","profile_url":"https://independent.academia.edu/AAlver?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_8821825 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="8821825"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 8821825, container: ".js-paper-rank-work_8821825", }); });</script></li><li class="js-percentile-work_8821825 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 8821825; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_8821825"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_8821825 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="8821825"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 8821825; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=8821825]").text(description); $(".js-view-count-work_8821825").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_8821825").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="8821825"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="647" rel="nofollow" href="https://www.academia.edu/Documents/in/Surgery">Surgery</a>, <script data-card-contents-for-ri="647" type="text/json">{"id":647,"name":"Surgery","url":"https://www.academia.edu/Documents/in/Surgery?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3243" rel="nofollow" href="https://www.academia.edu/Documents/in/Nonparametric_Statistics">Nonparametric Statistics</a>, <script data-card-contents-for-ri="3243" type="text/json">{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="8942" rel="nofollow" href="https://www.academia.edu/Documents/in/Treatment">Treatment</a>, <script data-card-contents-for-ri="8942" type="text/json">{"id":8942,"name":"Treatment","url":"https://www.academia.edu/Documents/in/Treatment?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=8821825]'), work: {"id":8821825,"title":"One-lung ventilation: For how long","created_at":"2014-10-16T20:57:08.650-07:00","url":"https://www.academia.edu/8821825/One_lung_ventilation_For_how_long?f_ri=55175","dom_id":"work_8821825","summary":"Objective: Lung injury induced by one-lung ventilation is rare, but it is a condition that may result in high mortality. This study evaluates the effects of one-lung ventilation and occlusion time on collapsed and contralateral lungs.","downloadable_attachments":[{"id":47990639,"asset_id":8821825,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":19161182,"first_name":"Ahmet","last_name":"Alver","domain_name":"independent","page_name":"AAlver","display_name":"Ahmet Alver","profile_url":"https://independent.academia.edu/AAlver?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":647,"name":"Surgery","url":"https://www.academia.edu/Documents/in/Surgery?f_ri=55175","nofollow":true},{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=55175","nofollow":true},{"id":8942,"name":"Treatment","url":"https://www.academia.edu/Documents/in/Treatment?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":102574,"name":"Thorax","url":"https://www.academia.edu/Documents/in/Thorax?f_ri=55175"},{"id":117674,"name":"Ventilation","url":"https://www.academia.edu/Documents/in/Ventilation?f_ri=55175"},{"id":131495,"name":"Heart","url":"https://www.academia.edu/Documents/in/Heart?f_ri=55175"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=55175"},{"id":233372,"name":"Lipid peroxidation","url":"https://www.academia.edu/Documents/in/Lipid_peroxidation?f_ri=55175"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":921372,"name":"One Lung Ventilation","url":"https://www.academia.edu/Documents/in/One_Lung_Ventilation?f_ri=55175"},{"id":1446339,"name":"Malondialdehyde","url":"https://www.academia.edu/Documents/in/Malondialdehyde?f_ri=55175"},{"id":1883354,"name":"Thoracic and cardiovascular surgery","url":"https://www.academia.edu/Documents/in/Thoracic_and_cardiovascular_surgery?f_ri=55175"},{"id":2463495,"name":"Random Allocation","url":"https://www.academia.edu/Documents/in/Random_Allocation?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_23962744" data-work_id="23962744" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/23962744/Ferulic_Acid_Dehydrodimers_in_Rye_Secale_cereale_L_">Ferulic Acid Dehydrodimers in Rye(Secale cereale L.)</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Four ferulic acid dehydrodimers were isolated from rye bran and purified by preparative HPLC after alkaline hydrolysis. The identity of the compounds were confirmed by UV, 1 H and 13 C-NMR and mass spectroscopy. The content of the four... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23962744" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Four ferulic acid dehydrodimers were isolated from rye bran and purified by preparative HPLC after alkaline hydrolysis. The identity of the compounds were confirmed by UV, 1 H and 13 C-NMR and mass spectroscopy. The content of the four identified dimers corresponded to a total dimer concentration of 307 g/g (dry matter) of the whole grain. The concentrations in the bran fraction were 10-20 times higher than in the starchy endosperm. The four dimers were in decreasing amounts:</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23962744" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="dbfe33cc5e9818e215e664f98d8800c4" rel="nofollow" data-download="{"attachment_id":44351897,"asset_id":23962744,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44351897/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35116476" href="https://independent.academia.edu/%C3%85Hansen">Åse Hansen</a><script data-card-contents-for-user="35116476" type="text/json">{"id":35116476,"first_name":"Åse","last_name":"Hansen","domain_name":"independent","page_name":"ÅHansen","display_name":"Åse Hansen","profile_url":"https://independent.academia.edu/%C3%85Hansen?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_23962744 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23962744"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23962744, container: ".js-paper-rank-work_23962744", }); });</script></li><li class="js-percentile-work_23962744 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23962744; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_23962744"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_23962744 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="23962744"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23962744; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23962744]").text(description); $(".js-view-count-work_23962744").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_23962744").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="23962744"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" rel="nofollow" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37434" rel="nofollow" href="https://www.academia.edu/Documents/in/Quantitative_analysis">Quantitative analysis</a>, <script data-card-contents-for-ri="37434" type="text/json">{"id":37434,"name":"Quantitative analysis","url":"https://www.academia.edu/Documents/in/Quantitative_analysis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=23962744]'), work: {"id":23962744,"title":"Ferulic Acid Dehydrodimers in Rye(Secale cereale L.)","created_at":"2016-04-03T05:25:29.215-07:00","url":"https://www.academia.edu/23962744/Ferulic_Acid_Dehydrodimers_in_Rye_Secale_cereale_L_?f_ri=55175","dom_id":"work_23962744","summary":"Four ferulic acid dehydrodimers were isolated from rye bran and purified by preparative HPLC after alkaline hydrolysis. The identity of the compounds were confirmed by UV, 1 H and 13 C-NMR and mass spectroscopy. The content of the four identified dimers corresponded to a total dimer concentration of 307 g/g (dry matter) of the whole grain. The concentrations in the bran fraction were 10-20 times higher than in the starchy endosperm. The four dimers were in decreasing amounts:","downloadable_attachments":[{"id":44351897,"asset_id":23962744,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35116476,"first_name":"Åse","last_name":"Hansen","domain_name":"independent","page_name":"ÅHansen","display_name":"Åse Hansen","profile_url":"https://independent.academia.edu/%C3%85Hansen?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true},{"id":37434,"name":"Quantitative analysis","url":"https://www.academia.edu/Documents/in/Quantitative_analysis?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":75847,"name":"HPLC","url":"https://www.academia.edu/Documents/in/HPLC?f_ri=55175"},{"id":214830,"name":"Cereal","url":"https://www.academia.edu/Documents/in/Cereal?f_ri=55175"},{"id":309895,"name":"Isolation","url":"https://www.academia.edu/Documents/in/Isolation?f_ri=55175"},{"id":413115,"name":"Peroxidases","url":"https://www.academia.edu/Documents/in/Peroxidases?f_ri=55175"},{"id":641216,"name":"Quantitative Analysis","url":"https://www.academia.edu/Documents/in/Quantitative_Analysis-1?f_ri=55175"},{"id":707350,"name":"Mass Spectroscopy","url":"https://www.academia.edu/Documents/in/Mass_Spectroscopy?f_ri=55175"},{"id":1324013,"name":"Cereal Science","url":"https://www.academia.edu/Documents/in/Cereal_Science?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22750522" data-work_id="22750522" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22750522/Myeloperoxidase_derived_2_chlorohexadecanal_forms_Schiff_bases_with_primary_amines_of_ethanolamine_glycerophospholipids_and_lysine">Myeloperoxidase-derived 2-chlorohexadecanal forms Schiff bases with primary amines of ethanolamine glycerophospholipids and lysine</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Numerous studies have suggested relationships between myeloperoxidase, inflammation, and atherosclerosis. MPO-derived reactive chlorinating species (RCS) attack membrane plasmalogens releasing ␣-chloro-fatty aldehydes (␣-Cl-FALDs)... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_22750522" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Numerous studies have suggested relationships between myeloperoxidase, inflammation, and atherosclerosis. MPO-derived reactive chlorinating species (RCS) attack membrane plasmalogens releasing ␣-chloro-fatty aldehydes (␣-Cl-FALDs) including 2-chlorohexadecanal (2-ClHDA). The molecular targets of ␣-Cl-FALDs are not known. The current study demonstrates 2-ClHDA adducts with ethanolamine glycerophospholipids and Fmoc-lysine. Utilizing electrospray ionization mass spectrometry, chlorinated adducts were observed that are apparent Schiff base adducts. Reduction of these Schiff base adducts with sodium cyanoborohydride resulted in a novel, stable adduct produced by the elimination of HCl. NMR further confirmed this structure. 2-ClHDA adducts with ethanolamine glycerophospholipids were also substrates for phospholipase D (PLD). The hydrolysis products were derivatized to pentafluorobenzoyl esters, and further structurally confirmed by GC-MS. Multiple molecular species of 2-ClHDA-N-modified ethanolamine glycerophospholipids were observed in endothelial cells treated with 2-ClHDA. These results show novel Schiff base adducts of ␣-Cl-FALDs with primary amines, which may represent an important fate of ␣-Cl-FALDs.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22750522" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="08b32dde2ada186432e4add0efac8144" rel="nofollow" data-download="{"attachment_id":43313267,"asset_id":22750522,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43313267/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="44302186" href="https://independent.academia.edu/HsuFongFu">Fong-Fu Hsu</a><script data-card-contents-for-user="44302186" type="text/json">{"id":44302186,"first_name":"Fong-Fu","last_name":"Hsu","domain_name":"independent","page_name":"HsuFongFu","display_name":"Fong-Fu Hsu","profile_url":"https://independent.academia.edu/HsuFongFu?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_22750522 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="22750522"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 22750522, container: ".js-paper-rank-work_22750522", }); });</script></li><li class="js-percentile-work_22750522 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 22750522; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_22750522"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_22750522 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="22750522"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 22750522; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=22750522]").text(description); $(".js-view-count-work_22750522").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_22750522").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="22750522"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="532" rel="nofollow" href="https://www.academia.edu/Documents/in/Physical_Chemistry">Physical Chemistry</a>, <script data-card-contents-for-ri="532" type="text/json">{"id":532,"name":"Physical Chemistry","url":"https://www.academia.edu/Documents/in/Physical_Chemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="71510" rel="nofollow" href="https://www.academia.edu/Documents/in/Endothelial_Cells">Endothelial Cells</a>, <script data-card-contents-for-ri="71510" type="text/json">{"id":71510,"name":"Endothelial Cells","url":"https://www.academia.edu/Documents/in/Endothelial_Cells?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="83838" rel="nofollow" href="https://www.academia.edu/Documents/in/Amines">Amines</a><script data-card-contents-for-ri="83838" type="text/json">{"id":83838,"name":"Amines","url":"https://www.academia.edu/Documents/in/Amines?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22750522]'), work: {"id":22750522,"title":"Myeloperoxidase-derived 2-chlorohexadecanal forms Schiff bases with primary amines of ethanolamine glycerophospholipids and lysine","created_at":"2016-03-03T06:48:26.323-08:00","url":"https://www.academia.edu/22750522/Myeloperoxidase_derived_2_chlorohexadecanal_forms_Schiff_bases_with_primary_amines_of_ethanolamine_glycerophospholipids_and_lysine?f_ri=55175","dom_id":"work_22750522","summary":"Numerous studies have suggested relationships between myeloperoxidase, inflammation, and atherosclerosis. MPO-derived reactive chlorinating species (RCS) attack membrane plasmalogens releasing ␣-chloro-fatty aldehydes (␣-Cl-FALDs) including 2-chlorohexadecanal (2-ClHDA). The molecular targets of ␣-Cl-FALDs are not known. The current study demonstrates 2-ClHDA adducts with ethanolamine glycerophospholipids and Fmoc-lysine. Utilizing electrospray ionization mass spectrometry, chlorinated adducts were observed that are apparent Schiff base adducts. Reduction of these Schiff base adducts with sodium cyanoborohydride resulted in a novel, stable adduct produced by the elimination of HCl. NMR further confirmed this structure. 2-ClHDA adducts with ethanolamine glycerophospholipids were also substrates for phospholipase D (PLD). The hydrolysis products were derivatized to pentafluorobenzoyl esters, and further structurally confirmed by GC-MS. Multiple molecular species of 2-ClHDA-N-modified ethanolamine glycerophospholipids were observed in endothelial cells treated with 2-ClHDA. These results show novel Schiff base adducts of ␣-Cl-FALDs with primary amines, which may represent an important fate of ␣-Cl-FALDs.","downloadable_attachments":[{"id":43313267,"asset_id":22750522,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":44302186,"first_name":"Fong-Fu","last_name":"Hsu","domain_name":"independent","page_name":"HsuFongFu","display_name":"Fong-Fu Hsu","profile_url":"https://independent.academia.edu/HsuFongFu?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":532,"name":"Physical Chemistry","url":"https://www.academia.edu/Documents/in/Physical_Chemistry?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":71510,"name":"Endothelial Cells","url":"https://www.academia.edu/Documents/in/Endothelial_Cells?f_ri=55175","nofollow":true},{"id":83838,"name":"Amines","url":"https://www.academia.edu/Documents/in/Amines?f_ri=55175","nofollow":true},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=55175"},{"id":232831,"name":"Schiff bases","url":"https://www.academia.edu/Documents/in/Schiff_bases?f_ri=55175"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=55175"},{"id":326380,"name":"Liquid Chromatography / Electrospray Ionization Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Liquid_Chromatography_Electrospray_Ionization_Mass_Spectrometry?f_ri=55175"},{"id":373650,"name":"Lysine","url":"https://www.academia.edu/Documents/in/Lysine?f_ri=55175"},{"id":401214,"name":"Endothelial cell","url":"https://www.academia.edu/Documents/in/Endothelial_cell?f_ri=55175"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=55175"},{"id":423179,"name":"Schiff Base","url":"https://www.academia.edu/Documents/in/Schiff_Base?f_ri=55175"},{"id":708643,"name":"Phospholipase D","url":"https://www.academia.edu/Documents/in/Phospholipase_D?f_ri=55175"},{"id":1030794,"name":"Hydrolysis","url":"https://www.academia.edu/Documents/in/Hydrolysis?f_ri=55175"},{"id":1401310,"name":"Myeloperoxidase","url":"https://www.academia.edu/Documents/in/Myeloperoxidase?f_ri=55175"},{"id":1724844,"name":"Molecular Structure","url":"https://www.academia.edu/Documents/in/Molecular_Structure?f_ri=55175"},{"id":1766065,"name":"Chemistry physics","url":"https://www.academia.edu/Documents/in/Chemistry_physics-1?f_ri=55175"},{"id":2230327,"name":"Aldehydes","url":"https://www.academia.edu/Documents/in/Aldehydes?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15910670" data-work_id="15910670" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15910670/Simultaneous_analysis_of_five_genetic_risk_factors_in_Polish_patients_with_Alzheimers_disease">Simultaneous analysis of five genetic risk factors in Polish patients with Alzheimer's disease</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">As Alzheimer's disease (AD) is a complex disease, we decided to estimate how previously reported genetic polymorphisms interact to increase the risk for the disease. Five candidate genes were chosen: apolipoprotein E (APOE),... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15910670" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">As Alzheimer's disease (AD) is a complex disease, we decided to estimate how previously reported genetic polymorphisms interact to increase the risk for the disease. Five candidate genes were chosen: apolipoprotein E (APOE), a2-macroglobulin, cathepsin D, myeloperoxidase and nitric oxide synthase. Genotyping was performed in 100 cases of late-onset AD and 100 healthy controls. We found a highly significant difference in APOE 14 distribution between groups (P , 0:005). However, no evidence of association for other studied loci was found. Cumulative analysis of five genetic polymorphisms was performed, but it also failed to reveal any synergistic effect of candidate genes greater than that caused by APOE itself. Our results suggest that the APOE 14 allele is the only known genetic risk factor for late-onset, sporadic AD. q</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15910670" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e1dceb5e705040861e2c80609f4822a3" rel="nofollow" data-download="{"attachment_id":42829395,"asset_id":15910670,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42829395/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35068572" href="https://ki.academia.edu/DorotaReliga">Dorota Religa</a><script data-card-contents-for-user="35068572" type="text/json">{"id":35068572,"first_name":"Dorota","last_name":"Religa","domain_name":"ki","page_name":"DorotaReliga","display_name":"Dorota Religa","profile_url":"https://ki.academia.edu/DorotaReliga?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_15910670 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="15910670"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 15910670, container: ".js-paper-rank-work_15910670", }); });</script></li><li class="js-percentile-work_15910670 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15910670; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_15910670"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_15910670 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="15910670"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15910670; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15910670]").text(description); $(".js-view-count-work_15910670").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_15910670").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="15910670"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="156" rel="nofollow" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="161" rel="nofollow" href="https://www.academia.edu/Documents/in/Neuroscience">Neuroscience</a>, <script data-card-contents-for-ri="161" type="text/json">{"id":161,"name":"Neuroscience","url":"https://www.academia.edu/Documents/in/Neuroscience?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="221" rel="nofollow" href="https://www.academia.edu/Documents/in/Psychology">Psychology</a>, <script data-card-contents-for-ri="221" type="text/json">{"id":221,"name":"Psychology","url":"https://www.academia.edu/Documents/in/Psychology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="237" rel="nofollow" href="https://www.academia.edu/Documents/in/Cognitive_Science">Cognitive Science</a><script data-card-contents-for-ri="237" type="text/json">{"id":237,"name":"Cognitive Science","url":"https://www.academia.edu/Documents/in/Cognitive_Science?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=15910670]'), work: {"id":15910670,"title":"Simultaneous analysis of five genetic risk factors in Polish patients with Alzheimer's disease","created_at":"2015-09-19T12:21:42.948-07:00","url":"https://www.academia.edu/15910670/Simultaneous_analysis_of_five_genetic_risk_factors_in_Polish_patients_with_Alzheimers_disease?f_ri=55175","dom_id":"work_15910670","summary":"As Alzheimer's disease (AD) is a complex disease, we decided to estimate how previously reported genetic polymorphisms interact to increase the risk for the disease. Five candidate genes were chosen: apolipoprotein E (APOE), a2-macroglobulin, cathepsin D, myeloperoxidase and nitric oxide synthase. Genotyping was performed in 100 cases of late-onset AD and 100 healthy controls. We found a highly significant difference in APOE 14 distribution between groups (P , 0:005). However, no evidence of association for other studied loci was found. Cumulative analysis of five genetic polymorphisms was performed, but it also failed to reveal any synergistic effect of candidate genes greater than that caused by APOE itself. Our results suggest that the APOE 14 allele is the only known genetic risk factor for late-onset, sporadic AD. q","downloadable_attachments":[{"id":42829395,"asset_id":15910670,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35068572,"first_name":"Dorota","last_name":"Religa","domain_name":"ki","page_name":"DorotaReliga","display_name":"Dorota Religa","profile_url":"https://ki.academia.edu/DorotaReliga?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=55175","nofollow":true},{"id":161,"name":"Neuroscience","url":"https://www.academia.edu/Documents/in/Neuroscience?f_ri=55175","nofollow":true},{"id":221,"name":"Psychology","url":"https://www.academia.edu/Documents/in/Psychology?f_ri=55175","nofollow":true},{"id":237,"name":"Cognitive Science","url":"https://www.academia.edu/Documents/in/Cognitive_Science?f_ri=55175","nofollow":true},{"id":3855,"name":"Polymorphism","url":"https://www.academia.edu/Documents/in/Polymorphism?f_ri=55175"},{"id":27363,"name":"Poland","url":"https://www.academia.edu/Documents/in/Poland?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":63186,"name":"Genetics of complex disease","url":"https://www.academia.edu/Documents/in/Genetics_of_complex_disease?f_ri=55175"},{"id":71477,"name":"cathepsin D","url":"https://www.academia.edu/Documents/in/cathepsin_D?f_ri=55175"},{"id":73153,"name":"Genetic Testing","url":"https://www.academia.edu/Documents/in/Genetic_Testing?f_ri=55175"},{"id":192721,"name":"Risk factors","url":"https://www.academia.edu/Documents/in/Risk_factors?f_ri=55175"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=55175"},{"id":382388,"name":"Nitric Oxide Synthase","url":"https://www.academia.edu/Documents/in/Nitric_Oxide_Synthase?f_ri=55175"},{"id":516151,"name":"Genetic Polymorphism","url":"https://www.academia.edu/Documents/in/Genetic_Polymorphism?f_ri=55175"},{"id":620049,"name":"Risk Factors","url":"https://www.academia.edu/Documents/in/Risk_Factors-1?f_ri=55175"},{"id":1120234,"name":"Alzheimer Disease","url":"https://www.academia.edu/Documents/in/Alzheimer_Disease?f_ri=55175"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=55175"},{"id":1401310,"name":"Myeloperoxidase","url":"https://www.academia.edu/Documents/in/Myeloperoxidase?f_ri=55175"},{"id":1993786,"name":"Cumulant","url":"https://www.academia.edu/Documents/in/Cumulant?f_ri=55175"},{"id":2228624,"name":"Synergistic effect","url":"https://www.academia.edu/Documents/in/Synergistic_effect?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16448666" data-work_id="16448666" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16448666/The_detection_of_cyclobutane_thymine_dimers_6_4_photolesions_and_the_Dewar_photoisomers_in_sections_of_UV_irradiated_human_skin_using_specific_antibodies_and_the_demonstration_of_depth_penetration_effects">The detection of cyclobutane thymine dimers, (6-4) photolesions and the Dewar photoisomers in sections of UV-irradiated human skin using specific antibodies, and the demonstration of depth penetration effects</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Ultraviolet irradiation of skin induces various DNA photolesions. Here we demonstrate that irradiation of human buttock skin with 300 nm UVR in situ induces thymine dimers and 6-4 photoproducts. Irradiation with 260 nm immediately... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16448666" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Ultraviolet irradiation of skin induces various DNA photolesions. Here we demonstrate that irradiation of human buttock skin with 300 nm UVR in situ induces thymine dimers and 6-4 photoproducts. Irradiation with 260 nm immediately followed by UVA (320 nm) induces the Dewar photoisomers of the 6-4 lesions. All three lesions can be detected in methanol-fixed paraffin sections using specific monoclonal antibodies.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16448666" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5ccf87e7271c513f777704b7a783ae83" rel="nofollow" data-download="{"attachment_id":42483152,"asset_id":16448666,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42483152/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35628062" href="https://independent.academia.edu/AntonyYoung">Antony Young</a><script data-card-contents-for-user="35628062" type="text/json">{"id":35628062,"first_name":"Antony","last_name":"Young","domain_name":"independent","page_name":"AntonyYoung","display_name":"Antony Young","profile_url":"https://independent.academia.edu/AntonyYoung?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_16448666 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16448666"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16448666, container: ".js-paper-rank-work_16448666", }); });</script></li><li class="js-percentile-work_16448666 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16448666; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_16448666"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16448666 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16448666"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16448666; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16448666]").text(description); $(".js-view-count-work_16448666").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16448666").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16448666"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="5104" rel="nofollow" href="https://www.academia.edu/Documents/in/Photochemistry">Photochemistry</a>, <script data-card-contents-for-ri="5104" type="text/json">{"id":5104,"name":"Photochemistry","url":"https://www.academia.edu/Documents/in/Photochemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9351" rel="nofollow" href="https://www.academia.edu/Documents/in/Image_Analysis">Image Analysis</a>, <script data-card-contents-for-ri="9351" type="text/json">{"id":9351,"name":"Image Analysis","url":"https://www.academia.edu/Documents/in/Image_Analysis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="44150" rel="nofollow" href="https://www.academia.edu/Documents/in/Integrated_Optics">Integrated Optics</a>, <script data-card-contents-for-ri="44150" type="text/json">{"id":44150,"name":"Integrated Optics","url":"https://www.academia.edu/Documents/in/Integrated_Optics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16448666]'), work: {"id":16448666,"title":"The detection of cyclobutane thymine dimers, (6-4) photolesions and the Dewar photoisomers in sections of UV-irradiated human skin using specific antibodies, and the demonstration of depth penetration effects","created_at":"2015-10-04T07:55:23.690-07:00","url":"https://www.academia.edu/16448666/The_detection_of_cyclobutane_thymine_dimers_6_4_photolesions_and_the_Dewar_photoisomers_in_sections_of_UV_irradiated_human_skin_using_specific_antibodies_and_the_demonstration_of_depth_penetration_effects?f_ri=55175","dom_id":"work_16448666","summary":"Ultraviolet irradiation of skin induces various DNA photolesions. Here we demonstrate that irradiation of human buttock skin with 300 nm UVR in situ induces thymine dimers and 6-4 photoproducts. Irradiation with 260 nm immediately followed by UVA (320 nm) induces the Dewar photoisomers of the 6-4 lesions. All three lesions can be detected in methanol-fixed paraffin sections using specific monoclonal antibodies.","downloadable_attachments":[{"id":42483152,"asset_id":16448666,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35628062,"first_name":"Antony","last_name":"Young","domain_name":"independent","page_name":"AntonyYoung","display_name":"Antony Young","profile_url":"https://independent.academia.edu/AntonyYoung?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5104,"name":"Photochemistry","url":"https://www.academia.edu/Documents/in/Photochemistry?f_ri=55175","nofollow":true},{"id":9351,"name":"Image Analysis","url":"https://www.academia.edu/Documents/in/Image_Analysis?f_ri=55175","nofollow":true},{"id":44150,"name":"Integrated Optics","url":"https://www.academia.edu/Documents/in/Integrated_Optics?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":87546,"name":"Ultraviolet","url":"https://www.academia.edu/Documents/in/Ultraviolet?f_ri=55175"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=55175"},{"id":162553,"name":"Skin","url":"https://www.academia.edu/Documents/in/Skin?f_ri=55175"},{"id":485597,"name":"UV irradiation","url":"https://www.academia.edu/Documents/in/UV_irradiation?f_ri=55175"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody?f_ri=55175"},{"id":1173577,"name":"Epidermal Stem Cells","url":"https://www.academia.edu/Documents/in/Epidermal_Stem_Cells?f_ri=55175"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21340067" data-work_id="21340067" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21340067/Inactivation_kinetics_of_peroxidase_in_zucchini_Cucurbita_pepo_L_by_heat_and_UV_C_radiation">Inactivation kinetics of peroxidase in zucchini ( Cucurbita pepo L.) by heat and UV-C radiation</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The effect of traditional thermal water blanching, or its combination with a UV-C radiation pre-treatment (11 J/m 2 ), on the inactivation kinetics of peroxidase in zucchini (Cucurbita pepo L.) was carried out in the temperature range of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21340067" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The effect of traditional thermal water blanching, or its combination with a UV-C radiation pre-treatment (11 J/m 2 ), on the inactivation kinetics of peroxidase in zucchini (Cucurbita pepo L.) was carried out in the temperature range of 80 to 98°C, and up to 3 min of processing time. Peroxidase inactivation started being noticed only at temperatures around 85°C. For both treatments, the inactivation kinetics followed a first order reaction with the Arrhenius model describing the temperature dependence of the reaction rate. The obtained kinetic parameters (k Heat 89.6°C = 7.37 × 10 − 7 ± 3.47 × 10 − 7 min − 1 and E a Heat = 925 ± 69 kJ mol − 1 ; k Heat 89.6°C + UV-C = 2.42 × 10 − 5 ± 6.58 × 10 − 6 min − 1 and E a Heat + UV-C = 596 ± 48.8 kJ mol − 1 ) showed that UV-C radiation had a significant contribution to increase the peroxidase degradation rate. These results will help to design new pre-processing conditions for the production of frozen zucchini, using less severe thermal treatments and attempt to minimize quality losses. Industrial relevance: The use of UV-C radiation in processing creates novel methodologies, which can be complementary to classical techniques, providing reduced processing times and increased efficiency. Having been proved to be particularly useful in sterilization of liquids and surfaces, inhibition of microbial population and in decrease of protein synthesis, its application in combination with the traditional blanching may be in favor of less severe heating treatments. The evaluation of the kinetics of zucchini (Cucurbita pepo L.) peroxidase inactivation with the traditional blanching method and combined with ultraviolet radiation suggests that short UV-C treatments may be a useful non-chemical pre-freezing method, allowing the application of shorter blanching times, leading to a product with eventual higher quality retention which is highly relevant to industry.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21340067" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8d6a9a992b9dd4367107dfdc587b9e27" rel="nofollow" data-download="{"attachment_id":41823806,"asset_id":21340067,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41823806/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42335637" href="https://ualg.academia.edu/MargaridaVieira">Margarida Vieira</a><script data-card-contents-for-user="42335637" type="text/json">{"id":42335637,"first_name":"Margarida","last_name":"Vieira","domain_name":"ualg","page_name":"MargaridaVieira","display_name":"Margarida Vieira","profile_url":"https://ualg.academia.edu/MargaridaVieira?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_21340067 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21340067"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21340067, container: ".js-paper-rank-work_21340067", }); });</script></li><li class="js-percentile-work_21340067 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21340067; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_21340067"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_21340067 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="21340067"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21340067; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21340067]").text(description); $(".js-view-count-work_21340067").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21340067").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="21340067"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" rel="nofollow" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21340067]'), work: {"id":21340067,"title":"Inactivation kinetics of peroxidase in zucchini ( Cucurbita pepo L.) by heat and UV-C radiation","created_at":"2016-01-31T15:32:39.580-08:00","url":"https://www.academia.edu/21340067/Inactivation_kinetics_of_peroxidase_in_zucchini_Cucurbita_pepo_L_by_heat_and_UV_C_radiation?f_ri=55175","dom_id":"work_21340067","summary":"The effect of traditional thermal water blanching, or its combination with a UV-C radiation pre-treatment (11 J/m 2 ), on the inactivation kinetics of peroxidase in zucchini (Cucurbita pepo L.) was carried out in the temperature range of 80 to 98°C, and up to 3 min of processing time. Peroxidase inactivation started being noticed only at temperatures around 85°C. For both treatments, the inactivation kinetics followed a first order reaction with the Arrhenius model describing the temperature dependence of the reaction rate. The obtained kinetic parameters (k Heat 89.6°C = 7.37 × 10 − 7 ± 3.47 × 10 − 7 min − 1 and E a Heat = 925 ± 69 kJ mol − 1 ; k Heat 89.6°C + UV-C = 2.42 × 10 − 5 ± 6.58 × 10 − 6 min − 1 and E a Heat + UV-C = 596 ± 48.8 kJ mol − 1 ) showed that UV-C radiation had a significant contribution to increase the peroxidase degradation rate. These results will help to design new pre-processing conditions for the production of frozen zucchini, using less severe thermal treatments and attempt to minimize quality losses. Industrial relevance: The use of UV-C radiation in processing creates novel methodologies, which can be complementary to classical techniques, providing reduced processing times and increased efficiency. Having been proved to be particularly useful in sterilization of liquids and surfaces, inhibition of microbial population and in decrease of protein synthesis, its application in combination with the traditional blanching may be in favor of less severe heating treatments. The evaluation of the kinetics of zucchini (Cucurbita pepo L.) peroxidase inactivation with the traditional blanching method and combined with ultraviolet radiation suggests that short UV-C treatments may be a useful non-chemical pre-freezing method, allowing the application of shorter blanching times, leading to a product with eventual higher quality retention which is highly relevant to industry.","downloadable_attachments":[{"id":41823806,"asset_id":21340067,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42335637,"first_name":"Margarida","last_name":"Vieira","domain_name":"ualg","page_name":"MargaridaVieira","display_name":"Margarida Vieira","profile_url":"https://ualg.academia.edu/MargaridaVieira?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":181847,"name":"First-Order Logic","url":"https://www.academia.edu/Documents/in/First-Order_Logic?f_ri=55175"},{"id":247487,"name":"Temperature Dependence","url":"https://www.academia.edu/Documents/in/Temperature_Dependence?f_ri=55175"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=55175"},{"id":394503,"name":"Thermal Treatment","url":"https://www.academia.edu/Documents/in/Thermal_Treatment?f_ri=55175"},{"id":778709,"name":"Reaction Rate","url":"https://www.academia.edu/Documents/in/Reaction_Rate?f_ri=55175"},{"id":1167882,"name":"Kinetic Parameter","url":"https://www.academia.edu/Documents/in/Kinetic_Parameter?f_ri=55175"},{"id":1449537,"name":"Ultrasounds","url":"https://www.academia.edu/Documents/in/Ultrasounds?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19040838" data-work_id="19040838" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19040838/Maintaining_the_quality_of_unripe_fresh_hazelnuts_through_storage_under_modified_atmospheres">Maintaining the quality of unripe, fresh hazelnuts through storage under modified atmospheres</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest">This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.</div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19040838" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="935279dad9014d3489993b9b944a1f95" rel="nofollow" data-download="{"attachment_id":42120737,"asset_id":19040838,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42120737/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39209516" href="https://independent.academia.edu/RobertoMoscetti">Roberto Moscetti</a><script data-card-contents-for-user="39209516" type="text/json">{"id":39209516,"first_name":"Roberto","last_name":"Moscetti","domain_name":"independent","page_name":"RobertoMoscetti","display_name":"Roberto Moscetti","profile_url":"https://independent.academia.edu/RobertoMoscetti?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_19040838 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19040838"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19040838, container: ".js-paper-rank-work_19040838", }); });</script></li><li class="js-percentile-work_19040838 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 19040838; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_19040838"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_19040838 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="19040838"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 19040838; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=19040838]").text(description); $(".js-view-count-work_19040838").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_19040838").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="19040838"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">5</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="4594" rel="nofollow" href="https://www.academia.edu/Documents/in/Carbon_Dioxide">Carbon Dioxide</a>, <script data-card-contents-for-ri="4594" type="text/json">{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="151091" rel="nofollow" href="https://www.academia.edu/Documents/in/Nitrogen">Nitrogen</a>, <script data-card-contents-for-ri="151091" type="text/json">{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="225187" rel="nofollow" href="https://www.academia.edu/Documents/in/Postharvest_Biology_and_Technology">Postharvest Biology and Technology</a><script data-card-contents-for-ri="225187" type="text/json">{"id":225187,"name":"Postharvest Biology and Technology","url":"https://www.academia.edu/Documents/in/Postharvest_Biology_and_Technology?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19040838]'), work: {"id":19040838,"title":"Maintaining the quality of unripe, fresh hazelnuts through storage under modified atmospheres","created_at":"2015-11-26T06:08:11.776-08:00","url":"https://www.academia.edu/19040838/Maintaining_the_quality_of_unripe_fresh_hazelnuts_through_storage_under_modified_atmospheres?f_ri=55175","dom_id":"work_19040838","summary":"This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.","downloadable_attachments":[{"id":42120737,"asset_id":19040838,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39209516,"first_name":"Roberto","last_name":"Moscetti","domain_name":"independent","page_name":"RobertoMoscetti","display_name":"Roberto Moscetti","profile_url":"https://independent.academia.edu/RobertoMoscetti?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen?f_ri=55175","nofollow":true},{"id":225187,"name":"Postharvest Biology and Technology","url":"https://www.academia.edu/Documents/in/Postharvest_Biology_and_Technology?f_ri=55175","nofollow":true},{"id":569759,"name":"Modified atmosphere packaging","url":"https://www.academia.edu/Documents/in/Modified_atmosphere_packaging?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1570247" data-work_id="1570247" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1570247/Peanut_priming_induced_by_biocontrol_agents">Peanut priming induced by biocontrol agents</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Selected strains of rhizobacteria induce systemic resistance in plant (ISR), enhancing the capacity to mobilize infection-induced cellular defense responses (priming). Bacillus sp. CHEP5 and Pseudomonas sp BREN6 strains reduced root and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_1570247" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Selected strains of rhizobacteria induce systemic resistance in plant (ISR), enhancing the capacity to mobilize infection-induced cellular defense responses (priming). Bacillus sp. CHEP5 and Pseudomonas sp BREN6 strains reduced root and stem wilt disease severity caused by Sclerotium rolfsii in Arachis hypogaea L. Strains inoculation increased the activity of phenylalanine ammonia-lyase and peroxidase, after pathogen-challenge, indicating priming. CHEP5 primes plants to produce more ethylene upon stimulation with ACC. A. hypogaea L is an ISR-positive plant and BREN6 and CHEP5 strains enhance the plant’s defense capacity by priming for potentiated activity of defense proteins and ACC-converting capacity.► A. hypogaea L. (peanut) is an ISR-positive plant. ► ISR response protects peanut from root and stem wilt caused by S. rolfsii. ► ISR is associated with an enhanced capacity to convert ACC to ethylene in systemic tissues.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/1570247" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="14bc7acdc67f44be5f886cc0005e901d" rel="nofollow" data-download="{"attachment_id":50926667,"asset_id":1570247,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50926667/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="1771778" href="https://amity.academia.edu/BhuvaneshwariVeluswamy">Bhuvaneshwari Veluswamy</a><script data-card-contents-for-user="1771778" type="text/json">{"id":1771778,"first_name":"Bhuvaneshwari","last_name":"Veluswamy","domain_name":"amity","page_name":"BhuvaneshwariVeluswamy","display_name":"Bhuvaneshwari Veluswamy","profile_url":"https://amity.academia.edu/BhuvaneshwariVeluswamy?f_ri=55175","photo":"https://0.academia-photos.com/1771778/607080/754143/s65_bhuvaneshwari.veluswamy.jpg"}</script></span></span></li><li class="js-paper-rank-work_1570247 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="1570247"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 1570247, container: ".js-paper-rank-work_1570247", }); });</script></li><li class="js-percentile-work_1570247 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 1570247; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_1570247"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_1570247 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="1570247"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 1570247; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=1570247]").text(description); $(".js-view-count-work_1570247").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_1570247").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="1570247"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="25657" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Molecular_Biology">Plant Molecular Biology</a>, <script data-card-contents-for-ri="25657" type="text/json">{"id":25657,"name":"Plant Molecular Biology","url":"https://www.academia.edu/Documents/in/Plant_Molecular_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=1570247]'), work: {"id":1570247,"title":"Peanut priming induced by biocontrol agents","created_at":"2012-05-18T03:30:24.397-07:00","url":"https://www.academia.edu/1570247/Peanut_priming_induced_by_biocontrol_agents?f_ri=55175","dom_id":"work_1570247","summary":"Selected strains of rhizobacteria induce systemic resistance in plant (ISR), enhancing the capacity to mobilize infection-induced cellular defense responses (priming). Bacillus sp. CHEP5 and Pseudomonas sp BREN6 strains reduced root and stem wilt disease severity caused by Sclerotium rolfsii in Arachis hypogaea L. Strains inoculation increased the activity of phenylalanine ammonia-lyase and peroxidase, after pathogen-challenge, indicating priming. CHEP5 primes plants to produce more ethylene upon stimulation with ACC. A. hypogaea L is an ISR-positive plant and BREN6 and CHEP5 strains enhance the plant’s defense capacity by priming for potentiated activity of defense proteins and ACC-converting capacity.► A. hypogaea L. (peanut) is an ISR-positive plant. ► ISR response protects peanut from root and stem wilt caused by S. rolfsii. ► ISR is associated with an enhanced capacity to convert ACC to ethylene in systemic tissues.","downloadable_attachments":[{"id":50926667,"asset_id":1570247,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":1771778,"first_name":"Bhuvaneshwari","last_name":"Veluswamy","domain_name":"amity","page_name":"BhuvaneshwariVeluswamy","display_name":"Bhuvaneshwari Veluswamy","profile_url":"https://amity.academia.edu/BhuvaneshwariVeluswamy?f_ri=55175","photo":"https://0.academia-photos.com/1771778/607080/754143/s65_bhuvaneshwari.veluswamy.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=55175","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true},{"id":25657,"name":"Plant Molecular Biology","url":"https://www.academia.edu/Documents/in/Plant_Molecular_Biology?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":137946,"name":"Disease Severity","url":"https://www.academia.edu/Documents/in/Disease_Severity?f_ri=55175"},{"id":1031781,"name":"Bacillus Sp","url":"https://www.academia.edu/Documents/in/Bacillus_Sp?f_ri=55175"},{"id":1136347,"name":"Induced Systemic Resistance","url":"https://www.academia.edu/Documents/in/Induced_Systemic_Resistance?f_ri=55175"},{"id":1493934,"name":"Phenylalanine ammonia lyase","url":"https://www.academia.edu/Documents/in/Phenylalanine_ammonia_lyase?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27693360" data-work_id="27693360" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27693360/The_anti_inflammatory_effects_and_the_inhibition_of_neutrophil_responsiveness_by_Barleria_lupulina_and_Clinacanthus_nutans_extracts">The anti-inflammatory effects and the inhibition of neutrophil responsiveness by Barleria lupulina and Clinacanthus nutans extracts</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Aim of the study: To investigate the anti-inflammatory activities of Barleria lupulina Lindl and Clinacanthus nutans (Burm.f.) Lindau extracts using two neutrophil-dependent acute inflammatory models and, in order to elucidate underlying... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_27693360" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Aim of the study: To investigate the anti-inflammatory activities of Barleria lupulina Lindl and Clinacanthus nutans (Burm.f.) Lindau extracts using two neutrophil-dependent acute inflammatory models and, in order to elucidate underlying cellular mechanisms, the effects of the extracts on human neutrophil responsiveness was investigated. Materials and methods: The in vivo inflammatory models examined were carrageenan-induced paw oedema and ethyl phenylpropiolate-induced ear oedema in rats. Myeloperoxidase (MPO) activity was assayed as an indicator of neutrophil migration. Human neutrophil functional responsiveness was determined by measuring fMLP-induced chemotaxis, superoxide anion generation (SAG), and release of MPO and elastase. Apoptosis was assessed morphologically and flow-cytometrically. Neutrophil viability was assessed by trypan blue exclusion and MTT cytotoxicity assays. Results: Both extracts induced powerful dose-dependent inhibitory effects in both edema models in rats. Importantly, there was a significant inhibition of MPO activity in the inflamed tissue indicating that the anti-inflammatory effect of the extracts is associated with reduced neutrophil migration. Although both extracts did not affect neutrophil viability or apoptosis, treatment of neutrophils with the extracts concentration-dependently inhibited fMLP-induced chemotaxis, SAG, and MPO and elastase release. Conclusions: These findings suggest that the powerful anti-inflammatory properties of Barleria lupulina Lindl and Clinacanthus nutans (Burm.f.) Lindau extracts are mediated, in part, by inhibition of neutrophil responsiveness.Barleria lupulina Lindl, Clinacanthus nutans (Burm. f.)Lindau; Oedema formation; Neutrophil responsiveness</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27693360" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d42023d8c4556177e71bbc2c48ca0ad8" rel="nofollow" data-download="{"attachment_id":47958955,"asset_id":27693360,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47958955/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="51928531" href="https://independent.academia.edu/AmpaiPanthong">Ampai Panthong</a><script data-card-contents-for-user="51928531" type="text/json">{"id":51928531,"first_name":"Ampai","last_name":"Panthong","domain_name":"independent","page_name":"AmpaiPanthong","display_name":"Ampai Panthong","profile_url":"https://independent.academia.edu/AmpaiPanthong?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_27693360 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="27693360"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 27693360, container: ".js-paper-rank-work_27693360", }); });</script></li><li class="js-percentile-work_27693360 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27693360; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_27693360"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_27693360 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="27693360"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27693360; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27693360]").text(description); $(".js-view-count-work_27693360").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_27693360").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="27693360"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="4083" rel="nofollow" href="https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine">Complementary and Alternative Medicine</a>, <script data-card-contents-for-ri="4083" type="text/json">{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14032" rel="nofollow" href="https://www.academia.edu/Documents/in/Ethnopharmacology">Ethnopharmacology</a>, <script data-card-contents-for-ri="14032" type="text/json">{"id":14032,"name":"Ethnopharmacology","url":"https://www.academia.edu/Documents/in/Ethnopharmacology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14080" rel="nofollow" href="https://www.academia.edu/Documents/in/Pharmacognosy">Pharmacognosy</a><script data-card-contents-for-ri="14080" type="text/json">{"id":14080,"name":"Pharmacognosy","url":"https://www.academia.edu/Documents/in/Pharmacognosy?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=27693360]'), work: {"id":27693360,"title":"The anti-inflammatory effects and the inhibition of neutrophil responsiveness by Barleria lupulina and Clinacanthus nutans extracts","created_at":"2016-08-10T17:36:46.237-07:00","url":"https://www.academia.edu/27693360/The_anti_inflammatory_effects_and_the_inhibition_of_neutrophil_responsiveness_by_Barleria_lupulina_and_Clinacanthus_nutans_extracts?f_ri=55175","dom_id":"work_27693360","summary":"Aim of the study: To investigate the anti-inflammatory activities of Barleria lupulina Lindl and Clinacanthus nutans (Burm.f.) Lindau extracts using two neutrophil-dependent acute inflammatory models and, in order to elucidate underlying cellular mechanisms, the effects of the extracts on human neutrophil responsiveness was investigated. Materials and methods: The in vivo inflammatory models examined were carrageenan-induced paw oedema and ethyl phenylpropiolate-induced ear oedema in rats. Myeloperoxidase (MPO) activity was assayed as an indicator of neutrophil migration. Human neutrophil functional responsiveness was determined by measuring fMLP-induced chemotaxis, superoxide anion generation (SAG), and release of MPO and elastase. Apoptosis was assessed morphologically and flow-cytometrically. Neutrophil viability was assessed by trypan blue exclusion and MTT cytotoxicity assays. Results: Both extracts induced powerful dose-dependent inhibitory effects in both edema models in rats. Importantly, there was a significant inhibition of MPO activity in the inflamed tissue indicating that the anti-inflammatory effect of the extracts is associated with reduced neutrophil migration. Although both extracts did not affect neutrophil viability or apoptosis, treatment of neutrophils with the extracts concentration-dependently inhibited fMLP-induced chemotaxis, SAG, and MPO and elastase release. Conclusions: These findings suggest that the powerful anti-inflammatory properties of Barleria lupulina Lindl and Clinacanthus nutans (Burm.f.) Lindau extracts are mediated, in part, by inhibition of neutrophil responsiveness.Barleria lupulina Lindl, Clinacanthus nutans (Burm. f.)Lindau; Oedema formation; Neutrophil responsiveness","downloadable_attachments":[{"id":47958955,"asset_id":27693360,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":51928531,"first_name":"Ampai","last_name":"Panthong","domain_name":"independent","page_name":"AmpaiPanthong","display_name":"Ampai Panthong","profile_url":"https://independent.academia.edu/AmpaiPanthong?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true},{"id":14032,"name":"Ethnopharmacology","url":"https://www.academia.edu/Documents/in/Ethnopharmacology?f_ri=55175","nofollow":true},{"id":14080,"name":"Pharmacognosy","url":"https://www.academia.edu/Documents/in/Pharmacognosy?f_ri=55175","nofollow":true},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":204350,"name":"Anti-inflammatory agents","url":"https://www.academia.edu/Documents/in/Anti-inflammatory_agents?f_ri=55175"},{"id":295260,"name":"Neutrophils","url":"https://www.academia.edu/Documents/in/Neutrophils?f_ri=55175"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":579360,"name":"Acanthaceae","url":"https://www.academia.edu/Documents/in/Acanthaceae?f_ri=55175"},{"id":707585,"name":"Anti Inflammatory Activity","url":"https://www.academia.edu/Documents/in/Anti_Inflammatory_Activity?f_ri=55175"},{"id":789968,"name":"Extract","url":"https://www.academia.edu/Documents/in/Extract?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_49725696" data-work_id="49725696" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/49725696/Floral_biology_and_breeding_system_of_pohutukawa_Metrosideros_excelsa_Myrtaceae_">Floral biology and breeding system of pohutukawa ( Metrosideros excelsa , Myrtaceae)</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The floral biology and breeding system of pohutukawa (Metrosideros excelsa, Myrtaceae) , a mass-flowering tree of northern New Zealand coastlines, were examined. Trees flower over a pea k period of 2 weeks, and compound inflorescence s... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_49725696" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The floral biology and breeding system of pohutukawa (Metrosideros excelsa, Myrtaceae) , a mass-flowering tree of northern New Zealand coastlines, were examined. Trees flower over a pea k period of 2 weeks, and compound inflorescence s contain an average of 14 .3 showy, hermaphrodite , red brush flowers that remain open for 7 days. A brie f female flower stage (mean duration 1 .3 d) is followed by the main hermaphrodite phase that lasts fo r 4 days. Neither dichogamy nor herkogamy is important in preventing pollen and stigma interference. Pollen is highly viable (93 .6%), and stigma receptivity extends for at least 9 days, as indicated by peroxidase activity, pollen germination, pollen tub e length 24 h after pollination, and seed set. Stigmati c exudate production appears to increase up to 5 days post-anthesis. On average, flowers produce 46 p 1 nectar per day, containing 18% (w/v) sucrose. Floral design and display of pohutukawa are consisten t with high levels of autogamous and geitonogamous self-pollination. Controlled pollination experiments were used to assess the effect of self-and crosspollen and a pollen mixture from five unrelated parents on capsule and seed production, and on pollen tube growth in seven trees. Three trees in the experiment were self-incompatible, as quantified by th e index of self-incompatibility (ISI), indicating that natural populations may consist of a mosaic of selfcompatible and incompatible individuals. Self-in-B9900 2</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/49725696" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a16692e7f7dde0f6b604e05805d15d26" rel="nofollow" data-download="{"attachment_id":67987717,"asset_id":49725696,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/67987717/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="192504831" href="https://independent.academia.edu/KevinGould8">Kevin Gould</a><script data-card-contents-for-user="192504831" type="text/json">{"id":192504831,"first_name":"Kevin","last_name":"Gould","domain_name":"independent","page_name":"KevinGould8","display_name":"Kevin Gould","profile_url":"https://independent.academia.edu/KevinGould8?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_49725696 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="49725696"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 49725696, container: ".js-paper-rank-work_49725696", }); });</script></li><li class="js-percentile-work_49725696 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 49725696; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_49725696"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_49725696 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="49725696"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 49725696; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=49725696]").text(description); $(".js-view-count-work_49725696").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_49725696").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="49725696"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="155" rel="nofollow" href="https://www.academia.edu/Documents/in/Evolutionary_Biology">Evolutionary Biology</a>, <script data-card-contents-for-ri="155" type="text/json">{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="156" rel="nofollow" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="41421" rel="nofollow" href="https://www.academia.edu/Documents/in/Seed_production">Seed production</a><script data-card-contents-for-ri="41421" type="text/json">{"id":41421,"name":"Seed production","url":"https://www.academia.edu/Documents/in/Seed_production?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=49725696]'), work: {"id":49725696,"title":"Floral biology and breeding system of pohutukawa ( Metrosideros excelsa , Myrtaceae)","created_at":"2021-07-10T23:29:25.063-07:00","url":"https://www.academia.edu/49725696/Floral_biology_and_breeding_system_of_pohutukawa_Metrosideros_excelsa_Myrtaceae_?f_ri=55175","dom_id":"work_49725696","summary":"The floral biology and breeding system of pohutukawa (Metrosideros excelsa, Myrtaceae) , a mass-flowering tree of northern New Zealand coastlines, were examined. Trees flower over a pea k period of 2 weeks, and compound inflorescence s contain an average of 14 .3 showy, hermaphrodite , red brush flowers that remain open for 7 days. A brie f female flower stage (mean duration 1 .3 d) is followed by the main hermaphrodite phase that lasts fo r 4 days. Neither dichogamy nor herkogamy is important in preventing pollen and stigma interference. Pollen is highly viable (93 .6%), and stigma receptivity extends for at least 9 days, as indicated by peroxidase activity, pollen germination, pollen tub e length 24 h after pollination, and seed set. Stigmati c exudate production appears to increase up to 5 days post-anthesis. On average, flowers produce 46 p 1 nectar per day, containing 18% (w/v) sucrose. Floral design and display of pohutukawa are consisten t with high levels of autogamous and geitonogamous self-pollination. Controlled pollination experiments were used to assess the effect of self-and crosspollen and a pollen mixture from five unrelated parents on capsule and seed production, and on pollen tube growth in seven trees. Three trees in the experiment were self-incompatible, as quantified by th e index of self-incompatibility (ISI), indicating that natural populations may consist of a mosaic of selfcompatible and incompatible individuals. Self-in-B9900 2","downloadable_attachments":[{"id":67987717,"asset_id":49725696,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":192504831,"first_name":"Kevin","last_name":"Gould","domain_name":"independent","page_name":"KevinGould8","display_name":"Kevin Gould","profile_url":"https://independent.academia.edu/KevinGould8?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology?f_ri=55175","nofollow":true},{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=55175","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true},{"id":41421,"name":"Seed production","url":"https://www.academia.edu/Documents/in/Seed_production?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":116108,"name":"New Zealand","url":"https://www.academia.edu/Documents/in/New_Zealand?f_ri=55175"},{"id":129138,"name":"Floral biology","url":"https://www.academia.edu/Documents/in/Floral_biology?f_ri=55175"},{"id":182962,"name":"Life Cycle","url":"https://www.academia.edu/Documents/in/Life_Cycle?f_ri=55175"},{"id":189750,"name":"POLLEN GERMINATION","url":"https://www.academia.edu/Documents/in/POLLEN_GERMINATION?f_ri=55175"},{"id":485998,"name":"Inbreeding Depression","url":"https://www.academia.edu/Documents/in/Inbreeding_Depression?f_ri=55175"},{"id":992670,"name":"Pollen Tube Growth","url":"https://www.academia.edu/Documents/in/Pollen_Tube_Growth?f_ri=55175"},{"id":1005211,"name":"Breeding System","url":"https://www.academia.edu/Documents/in/Breeding_System?f_ri=55175"},{"id":1759331,"name":"Pollen tube","url":"https://www.academia.edu/Documents/in/Pollen_tube?f_ri=55175"},{"id":3591148,"name":"seed set","url":"https://www.academia.edu/Documents/in/seed_set?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_32997687" data-work_id="32997687" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/32997687/Oxidation_of_pyocyanin_a_cytotoxic_product_from_Pseudomonas_aeruginosa_by_microperoxidase_11_and_hydrogen_peroxide">Oxidation of pyocyanin, a cytotoxic product from Pseudomonas aeruginosa, by microperoxidase 11 and hydrogen peroxide</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Pyocyanin (1-hydroxy-N-methylphenazine) is a cytotoxic pigment secreted by the bacterial species Pseudomonas aeruginosa, which frequently infects the lungs of immunosuppressed patients as well as those with cystic fibrosis. Pyocyanin... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_32997687" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Pyocyanin (1-hydroxy-N-methylphenazine) is a cytotoxic pigment secreted by the bacterial species Pseudomonas aeruginosa, which frequently infects the lungs of immunosuppressed patients as well as those with cystic fibrosis. Pyocyanin toxicity results presumably from the ability of the compound to undergo reduction by NAD(P)H and subsequent generation of superoxide and H 2 O 2 directly in the lungs. We report that in the presence of peroxidase mimics, microperoxidase 11, or hemin, pyocyanin undergoes oxidation by H 2 O 2 , as evidenced by loss of the pigment's characteristic absorption spectrum and by EPR detection of a free radical metabolite. The oxidation of pyocyanin is irreversible, suggesting an extensive modification of the pigment's phenazine chromophore. Oxidation of pyocyanin was observed also when exogenous H 2 O 2 was replaced by a H 2 O 2 -generating system consisting of NADH and the pigment itself. That the oxidation involves the phenolate group of pyocyanin was verified by the observation that a related pigment, phenazine methosulfate, which is devoid of this group, does not undergo oxidation by microperoxidase 11/H 2 O 2 . In contrast to intact pyocyanin, oxidized pyocyanin was less efficient in NADH oxidation and stimulation of interleukin-8 release by human alveolar epithelial A549 cells in vitro, suggesting that oxidation of pyocyanin leads to its inactivation. This study demonstrates that pyocyanin may play a dual role in biological systems, first as an oxidant and ROS generator, and second as a substrate for peroxidases, contributing to H 2 O 2 removal. This latter property may cause pyocyanin degradation and inactivation, which may be of considerable biomedical interest. D 2004 Elsevier Inc. All rights reserved.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/32997687" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="120e14326f5bfb8f6c432a52ea03ef3c" rel="nofollow" data-download="{"attachment_id":53113373,"asset_id":32997687,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/53113373/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4848409" href="https://independent.academia.edu/MichaelMcCormick1">Michael McCormick</a><script data-card-contents-for-user="4848409" type="text/json">{"id":4848409,"first_name":"Michael","last_name":"McCormick","domain_name":"independent","page_name":"MichaelMcCormick1","display_name":"Michael McCormick","profile_url":"https://independent.academia.edu/MichaelMcCormick1?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_32997687 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="32997687"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 32997687, container: ".js-paper-rank-work_32997687", }); });</script></li><li class="js-percentile-work_32997687 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 32997687; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_32997687"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_32997687 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="32997687"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 32997687; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=32997687]").text(description); $(".js-view-count-work_32997687").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_32997687").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="32997687"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">21</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2425" rel="nofollow" href="https://www.academia.edu/Documents/in/Free_Radicals">Free Radicals</a>, <script data-card-contents-for-ri="2425" type="text/json">{"id":2425,"name":"Free Radicals","url":"https://www.academia.edu/Documents/in/Free_Radicals?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6946" rel="nofollow" href="https://www.academia.edu/Documents/in/Cystic_Fibrosis">Cystic Fibrosis</a>, <script data-card-contents-for-ri="6946" type="text/json">{"id":6946,"name":"Cystic Fibrosis","url":"https://www.academia.edu/Documents/in/Cystic_Fibrosis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="44022" rel="nofollow" href="https://www.academia.edu/Documents/in/Free_Radical">Free Radical</a>, <script data-card-contents-for-ri="44022" type="text/json">{"id":44022,"name":"Free Radical","url":"https://www.academia.edu/Documents/in/Free_Radical?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=32997687]'), work: {"id":32997687,"title":"Oxidation of pyocyanin, a cytotoxic product from Pseudomonas aeruginosa, by microperoxidase 11 and hydrogen peroxide","created_at":"2017-05-13T04:24:45.210-07:00","url":"https://www.academia.edu/32997687/Oxidation_of_pyocyanin_a_cytotoxic_product_from_Pseudomonas_aeruginosa_by_microperoxidase_11_and_hydrogen_peroxide?f_ri=55175","dom_id":"work_32997687","summary":"Pyocyanin (1-hydroxy-N-methylphenazine) is a cytotoxic pigment secreted by the bacterial species Pseudomonas aeruginosa, which frequently infects the lungs of immunosuppressed patients as well as those with cystic fibrosis. Pyocyanin toxicity results presumably from the ability of the compound to undergo reduction by NAD(P)H and subsequent generation of superoxide and H 2 O 2 directly in the lungs. We report that in the presence of peroxidase mimics, microperoxidase 11, or hemin, pyocyanin undergoes oxidation by H 2 O 2 , as evidenced by loss of the pigment's characteristic absorption spectrum and by EPR detection of a free radical metabolite. The oxidation of pyocyanin is irreversible, suggesting an extensive modification of the pigment's phenazine chromophore. Oxidation of pyocyanin was observed also when exogenous H 2 O 2 was replaced by a H 2 O 2 -generating system consisting of NADH and the pigment itself. That the oxidation involves the phenolate group of pyocyanin was verified by the observation that a related pigment, phenazine methosulfate, which is devoid of this group, does not undergo oxidation by microperoxidase 11/H 2 O 2 . In contrast to intact pyocyanin, oxidized pyocyanin was less efficient in NADH oxidation and stimulation of interleukin-8 release by human alveolar epithelial A549 cells in vitro, suggesting that oxidation of pyocyanin leads to its inactivation. This study demonstrates that pyocyanin may play a dual role in biological systems, first as an oxidant and ROS generator, and second as a substrate for peroxidases, contributing to H 2 O 2 removal. This latter property may cause pyocyanin degradation and inactivation, which may be of considerable biomedical interest. D 2004 Elsevier Inc. All rights reserved.","downloadable_attachments":[{"id":53113373,"asset_id":32997687,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4848409,"first_name":"Michael","last_name":"McCormick","domain_name":"independent","page_name":"MichaelMcCormick1","display_name":"Michael McCormick","profile_url":"https://independent.academia.edu/MichaelMcCormick1?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2425,"name":"Free Radicals","url":"https://www.academia.edu/Documents/in/Free_Radicals?f_ri=55175","nofollow":true},{"id":6946,"name":"Cystic Fibrosis","url":"https://www.academia.edu/Documents/in/Cystic_Fibrosis?f_ri=55175","nofollow":true},{"id":44022,"name":"Free Radical","url":"https://www.academia.edu/Documents/in/Free_Radical?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=55175"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=55175"},{"id":225499,"name":"Pseudomonas aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_aeruginosa?f_ri=55175"},{"id":259090,"name":"Oxidative stress and free radical biology and medicine","url":"https://www.academia.edu/Documents/in/Oxidative_stress_and_free_radical_biology_and_medicine?f_ri=55175"},{"id":273338,"name":"IL","url":"https://www.academia.edu/Documents/in/IL?f_ri=55175"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide?f_ri=55175"},{"id":321836,"name":"Spectrum","url":"https://www.academia.edu/Documents/in/Spectrum?f_ri=55175"},{"id":351461,"name":"Biological systems","url":"https://www.academia.edu/Documents/in/Biological_systems?f_ri=55175"},{"id":379416,"name":"Epithelial cells","url":"https://www.academia.edu/Documents/in/Epithelial_cells?f_ri=55175"},{"id":413115,"name":"Peroxidases","url":"https://www.academia.edu/Documents/in/Peroxidases?f_ri=55175"},{"id":563382,"name":"Oxidation","url":"https://www.academia.edu/Documents/in/Oxidation?f_ri=55175"},{"id":636395,"name":"Pseudomonas Aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_Aeruginosa-1?f_ri=55175"},{"id":828507,"name":"NAD","url":"https://www.academia.edu/Documents/in/NAD?f_ri=55175"},{"id":1199367,"name":"A","url":"https://www.academia.edu/Documents/in/A?f_ri=55175"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=55175"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=55175"},{"id":2565469,"name":"ah","url":"https://www.academia.edu/Documents/in/ah?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_31371021" data-work_id="31371021" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/31371021/Peroxidase_isozyme_polymorphism_in_Cucurbita_pepo_cultivars_with_various_morphotypes_and_different_level_of_field_resistance_to_powdery_mildew">Peroxidase isozyme polymorphism in Cucurbita pepo cultivars with various morphotypes and different level of field resistance to powdery mildew</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A set of 31 Cucurbita pepo L. commercial cultivars with different fruit shapes (morphotypes) was evaluated for resistance to powdery mildew of cucurbits (Erysiphe cichoracearum and Sphaerotheca fuliginea) under field conditions of a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_31371021" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A set of 31 Cucurbita pepo L. commercial cultivars with different fruit shapes (morphotypes) was evaluated for resistance to powdery mildew of cucurbits (Erysiphe cichoracearum and Sphaerotheca fuliginea) under field conditions of a natural infection. During vegetative growth, the infection degree (ID) was assessed four times on leaves (L) and twice on stems and petioles (S). Thè Area below the curve' values of the disease infection progress were calculated separately for leaves (ABC-L) and stems and petioles (ABC-S). Data were subjected to one-way analyses of variance and mean separation was performed using LSD multiple range tests. Significant differences in field resistance were found between morphotypes. Cultivars with scallop and acorn fruit shapes were the most resistant. The straightneck, crookneck and ornamental gourd groups showed medium levels of field resistance. The zucchini, cocozelle, pumpkin and vegetable marrow groups of morphotypes were the most susceptible. Isozyme spectra of peroxidases for 11 selected C. pepo cultivars representing different groups of morphotypes with various levels of field resistance were analysed as well. They could be ranged into three basic groups which correspond with the cultivar's level of field resistance. This phenomenon is discussed as a potential biochemical marker in C. pepo selection. # Abbreviations: ABC, Area below the curve;ID, Infection degree; L, Leaves; S, Stems and petioles 0304-4238/99/$ ± see front matter # 1999 Elsevier Science B.V. All rights reserved. PII: S 0 3 0 4 -4 2 3 8 ( 9 8 ) 0 0 2 6 1 -1 104</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/31371021" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="31955b2457d0e9af47c567b4ffcb5a06" rel="nofollow" data-download="{"attachment_id":51749195,"asset_id":31371021,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/51749195/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="59950937" href="https://independent.academia.edu/ValeriaLetkova">Valeria Letkova</a><script data-card-contents-for-user="59950937" type="text/json">{"id":59950937,"first_name":"Valeria","last_name":"Letkova","domain_name":"independent","page_name":"ValeriaLetkova","display_name":"Valeria Letkova","profile_url":"https://independent.academia.edu/ValeriaLetkova?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_31371021 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="31371021"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 31371021, container: ".js-paper-rank-work_31371021", }); });</script></li><li class="js-percentile-work_31371021 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 31371021; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_31371021"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_31371021 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="31371021"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 31371021; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=31371021]").text(description); $(".js-view-count-work_31371021").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_31371021").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="31371021"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">4</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="3855" rel="nofollow" href="https://www.academia.edu/Documents/in/Polymorphism">Polymorphism</a>, <script data-card-contents-for-ri="3855" type="text/json">{"id":3855,"name":"Polymorphism","url":"https://www.academia.edu/Documents/in/Polymorphism?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="553606" rel="nofollow" href="https://www.academia.edu/Documents/in/Powdery_Mildew">Powdery Mildew</a>, <script data-card-contents-for-ri="553606" type="text/json">{"id":553606,"name":"Powdery Mildew","url":"https://www.academia.edu/Documents/in/Powdery_Mildew?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1933056" rel="nofollow" href="https://www.academia.edu/Documents/in/Vegetative_Growth">Vegetative Growth</a><script data-card-contents-for-ri="1933056" type="text/json">{"id":1933056,"name":"Vegetative Growth","url":"https://www.academia.edu/Documents/in/Vegetative_Growth?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=31371021]'), work: {"id":31371021,"title":"Peroxidase isozyme polymorphism in Cucurbita pepo cultivars with various morphotypes and different level of field resistance to powdery mildew","created_at":"2017-02-11T06:03:35.979-08:00","url":"https://www.academia.edu/31371021/Peroxidase_isozyme_polymorphism_in_Cucurbita_pepo_cultivars_with_various_morphotypes_and_different_level_of_field_resistance_to_powdery_mildew?f_ri=55175","dom_id":"work_31371021","summary":"A set of 31 Cucurbita pepo L. commercial cultivars with different fruit shapes (morphotypes) was evaluated for resistance to powdery mildew of cucurbits (Erysiphe cichoracearum and Sphaerotheca fuliginea) under field conditions of a natural infection. During vegetative growth, the infection degree (ID) was assessed four times on leaves (L) and twice on stems and petioles (S). Thè Area below the curve' values of the disease infection progress were calculated separately for leaves (ABC-L) and stems and petioles (ABC-S). Data were subjected to one-way analyses of variance and mean separation was performed using LSD multiple range tests. Significant differences in field resistance were found between morphotypes. Cultivars with scallop and acorn fruit shapes were the most resistant. The straightneck, crookneck and ornamental gourd groups showed medium levels of field resistance. The zucchini, cocozelle, pumpkin and vegetable marrow groups of morphotypes were the most susceptible. Isozyme spectra of peroxidases for 11 selected C. pepo cultivars representing different groups of morphotypes with various levels of field resistance were analysed as well. They could be ranged into three basic groups which correspond with the cultivar's level of field resistance. This phenomenon is discussed as a potential biochemical marker in C. pepo selection. # Abbreviations: ABC, Area below the curve;ID, Infection degree; L, Leaves; S, Stems and petioles 0304-4238/99/$ ± see front matter # 1999 Elsevier Science B.V. All rights reserved. PII: S 0 3 0 4 -4 2 3 8 ( 9 8 ) 0 0 2 6 1 -1 104","downloadable_attachments":[{"id":51749195,"asset_id":31371021,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":59950937,"first_name":"Valeria","last_name":"Letkova","domain_name":"independent","page_name":"ValeriaLetkova","display_name":"Valeria Letkova","profile_url":"https://independent.academia.edu/ValeriaLetkova?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3855,"name":"Polymorphism","url":"https://www.academia.edu/Documents/in/Polymorphism?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":553606,"name":"Powdery Mildew","url":"https://www.academia.edu/Documents/in/Powdery_Mildew?f_ri=55175","nofollow":true},{"id":1933056,"name":"Vegetative Growth","url":"https://www.academia.edu/Documents/in/Vegetative_Growth?f_ri=55175","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13187563 coauthored" data-work_id="13187563" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13187563/Lignocellulose_degrading_enzyme_production_by_white_rot_Basidiomycetes_isolated_from_the_forests_of_Georgia">Lignocellulose-degrading enzyme production by white-rot Basidiomycetes isolated from the forests of Georgia</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The production of lignocellulolytic enzymes by eleven basidiomycetes species isolated from two ecosystems of Georgia was investigated for the first time under submerged (SF) and solid-state fermentation (SSF) of lignocellulosic... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13187563" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The production of lignocellulolytic enzymes by eleven basidiomycetes species isolated from two ecosystems of Georgia was investigated for the first time under submerged (SF) and solid-state fermentation (SSF) of lignocellulosic by-products. Notable intergeneric and intrageneric differences were revealed with regard to the extent of hydrolase and oxidase activity. Several fungi produced laccase along with hydrolases in parallel with growth during the trophophase, showing that the synthesis of this enzyme is not connected with secondary metabolism. The lignocellulosic substrate type had the greatest impact on enzyme secretion. Some of the substrates significantly stimulated lignocellulolytic enzyme synthesis without supplementation of the culture medium with specific inducers. Exceptionally high carboxymethyl cellulase (CMCase, 122 U ml -1 ) and xylanase (195 U ml -1 ) activities were revealed in SF of mandarin peelings by Pseudotremella gibbosa IBB 22 and of residue after ethanol production (REP) by Fomes fomentarius IBB 38, respectively. The SSF of REP by T. pubescens IBB 11 ensured the highest level of laccase activity (24,690 U l -1 ), whereas the SSF of wheat bran and SF of mandarin peels provided the highest manganese peroxidase activity (570-620 U l -1 ) of Trichaptum biforme IBB 117. Moreover, the variation of lignocellulosic growth substrate provides an opportunity to obtain enzyme preparations containing different ratios of individual enzymes.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13187563" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d52db545c255f03f03c962547d83becc" rel="nofollow" data-download="{"attachment_id":45608195,"asset_id":13187563,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45608195/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="7663577" href="https://independent.academia.edu/NinoTsiklauri">Nino Tsiklauri</a><script data-card-contents-for-user="7663577" type="text/json">{"id":7663577,"first_name":"Nino","last_name":"Tsiklauri","domain_name":"independent","page_name":"NinoTsiklauri","display_name":"Nino Tsiklauri","profile_url":"https://independent.academia.edu/NinoTsiklauri?f_ri=55175","photo":"https://0.academia-photos.com/7663577/36433447/31204358/s65_nino.tsiklauri.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13187563">+1</span><div class="hidden js-additional-users-13187563"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://uclouvain.academia.edu/SpirosNAgathos">Spiros N. Agathos</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13187563'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13187563').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_13187563 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13187563"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13187563, container: ".js-paper-rank-work_13187563", }); });</script></li><li class="js-percentile-work_13187563 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13187563; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_13187563"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_13187563 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="13187563"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13187563; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13187563]").text(description); $(".js-view-count-work_13187563").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_13187563").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="13187563"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="55" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_Engineering">Environmental Engineering</a>, <script data-card-contents-for-ri="55" type="text/json">{"id":55,"name":"Environmental Engineering","url":"https://www.academia.edu/Documents/in/Environmental_Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="72" rel="nofollow" href="https://www.academia.edu/Documents/in/Chemical_Engineering">Chemical Engineering</a>, <script data-card-contents-for-ri="72" type="text/json">{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="923" rel="nofollow" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13187563]'), work: {"id":13187563,"title":"Lignocellulose-degrading enzyme production by white-rot Basidiomycetes isolated from the forests of Georgia","created_at":"2015-06-22T16:35:41.615-07:00","url":"https://www.academia.edu/13187563/Lignocellulose_degrading_enzyme_production_by_white_rot_Basidiomycetes_isolated_from_the_forests_of_Georgia?f_ri=55175","dom_id":"work_13187563","summary":"The production of lignocellulolytic enzymes by eleven basidiomycetes species isolated from two ecosystems of Georgia was investigated for the first time under submerged (SF) and solid-state fermentation (SSF) of lignocellulosic by-products. Notable intergeneric and intrageneric differences were revealed with regard to the extent of hydrolase and oxidase activity. Several fungi produced laccase along with hydrolases in parallel with growth during the trophophase, showing that the synthesis of this enzyme is not connected with secondary metabolism. The lignocellulosic substrate type had the greatest impact on enzyme secretion. Some of the substrates significantly stimulated lignocellulolytic enzyme synthesis without supplementation of the culture medium with specific inducers. Exceptionally high carboxymethyl cellulase (CMCase, 122 U ml -1 ) and xylanase (195 U ml -1 ) activities were revealed in SF of mandarin peelings by Pseudotremella gibbosa IBB 22 and of residue after ethanol production (REP) by Fomes fomentarius IBB 38, respectively. The SSF of REP by T. pubescens IBB 11 ensured the highest level of laccase activity (24,690 U l -1 ), whereas the SSF of wheat bran and SF of mandarin peels provided the highest manganese peroxidase activity (570-620 U l -1 ) of Trichaptum biforme IBB 117. Moreover, the variation of lignocellulosic growth substrate provides an opportunity to obtain enzyme preparations containing different ratios of individual enzymes.","downloadable_attachments":[{"id":45608195,"asset_id":13187563,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":7663577,"first_name":"Nino","last_name":"Tsiklauri","domain_name":"independent","page_name":"NinoTsiklauri","display_name":"Nino Tsiklauri","profile_url":"https://independent.academia.edu/NinoTsiklauri?f_ri=55175","photo":"https://0.academia-photos.com/7663577/36433447/31204358/s65_nino.tsiklauri.jpg"},{"id":325118,"first_name":"Spiros N.","last_name":"Agathos","domain_name":"uclouvain","page_name":"SpirosNAgathos","display_name":"Spiros N. Agathos","profile_url":"https://uclouvain.academia.edu/SpirosNAgathos?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":55,"name":"Environmental Engineering","url":"https://www.academia.edu/Documents/in/Environmental_Engineering?f_ri=55175","nofollow":true},{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=55175","nofollow":true},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":63128,"name":"Solid State Fermentation","url":"https://www.academia.edu/Documents/in/Solid_State_Fermentation?f_ri=55175"},{"id":73606,"name":"World","url":"https://www.academia.edu/Documents/in/World?f_ri=55175"},{"id":94697,"name":"Plant Secondary Metabolism","url":"https://www.academia.edu/Documents/in/Plant_Secondary_Metabolism?f_ri=55175"},{"id":121414,"name":"Ethanol Production","url":"https://www.academia.edu/Documents/in/Ethanol_Production?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":1388620,"name":"Wheat Bran","url":"https://www.academia.edu/Documents/in/Wheat_Bran?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14151300" data-work_id="14151300" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14151300/A_systematic_review_of_human_antioxidant_genes">A systematic review of human antioxidant genes</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14151300" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3b2370d50eab7f7c7ee4a2600620d738" rel="nofollow" data-download="{"attachment_id":44560388,"asset_id":14151300,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44560388/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33140920" href="https://ufrgs.academia.edu/DanielGelain">Daniel Gelain</a><script data-card-contents-for-user="33140920" type="text/json">{"id":33140920,"first_name":"Daniel","last_name":"Gelain","domain_name":"ufrgs","page_name":"DanielGelain","display_name":"Daniel Gelain","profile_url":"https://ufrgs.academia.edu/DanielGelain?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_14151300 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="14151300"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 14151300, container: ".js-paper-rank-work_14151300", }); });</script></li><li class="js-percentile-work_14151300 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 14151300; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_14151300"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_14151300 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="14151300"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 14151300; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=14151300]").text(description); $(".js-view-count-work_14151300").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_14151300").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="14151300"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="51711" rel="nofollow" href="https://www.academia.edu/Documents/in/Antioxidants">Antioxidants</a>, <script data-card-contents-for-ri="51711" type="text/json">{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="213343" rel="nofollow" href="https://www.academia.edu/Documents/in/Superoxide_Dismutase">Superoxide Dismutase</a>, <script data-card-contents-for-ri="213343" type="text/json">{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="221411" rel="nofollow" href="https://www.academia.edu/Documents/in/Systematic_review">Systematic review</a><script data-card-contents-for-ri="221411" type="text/json">{"id":221411,"name":"Systematic review","url":"https://www.academia.edu/Documents/in/Systematic_review?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=14151300]'), work: {"id":14151300,"title":"A systematic review of human antioxidant genes","created_at":"2015-07-17T08:54:40.819-07:00","url":"https://www.academia.edu/14151300/A_systematic_review_of_human_antioxidant_genes?f_ri=55175","dom_id":"work_14151300","summary":null,"downloadable_attachments":[{"id":44560388,"asset_id":14151300,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33140920,"first_name":"Daniel","last_name":"Gelain","domain_name":"ufrgs","page_name":"DanielGelain","display_name":"Daniel Gelain","profile_url":"https://ufrgs.academia.edu/DanielGelain?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175","nofollow":true},{"id":221411,"name":"Systematic review","url":"https://www.academia.edu/Documents/in/Systematic_review?f_ri=55175","nofollow":true},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=55175"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_17244196" data-work_id="17244196" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/17244196/Indirect_peroxidase_staining_using_monoclonal_antibodies_specific_for_Mya_arenaria_neoplastic_cells">Indirect peroxidase staining using monoclonal antibodies specific for Mya arenaria neoplastic cells</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17244196" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4acb056a4cc8bca9873634af78fec892" rel="nofollow" data-download="{"attachment_id":42285466,"asset_id":17244196,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42285466/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="36774202" href="https://independent.academia.edu/RoxannaSmolowitz">Roxanna Smolowitz</a><script data-card-contents-for-user="36774202" type="text/json">{"id":36774202,"first_name":"Roxanna","last_name":"Smolowitz","domain_name":"independent","page_name":"RoxannaSmolowitz","display_name":"Roxanna Smolowitz","profile_url":"https://independent.academia.edu/RoxannaSmolowitz?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_17244196 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="17244196"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 17244196, container: ".js-paper-rank-work_17244196", }); });</script></li><li class="js-percentile-work_17244196 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17244196; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_17244196"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_17244196 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="17244196"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17244196; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17244196]").text(description); $(".js-view-count-work_17244196").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_17244196").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="17244196"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="173" rel="nofollow" href="https://www.academia.edu/Documents/in/Zoology">Zoology</a>, <script data-card-contents-for-ri="173" type="text/json">{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="85920" rel="nofollow" href="https://www.academia.edu/Documents/in/Bivalvia">Bivalvia</a>, <script data-card-contents-for-ri="85920" type="text/json">{"id":85920,"name":"Bivalvia","url":"https://www.academia.edu/Documents/in/Bivalvia?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="146990" rel="nofollow" href="https://www.academia.edu/Documents/in/Invertebrate_Pathology">Invertebrate Pathology</a><script data-card-contents-for-ri="146990" type="text/json">{"id":146990,"name":"Invertebrate Pathology","url":"https://www.academia.edu/Documents/in/Invertebrate_Pathology?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=17244196]'), work: {"id":17244196,"title":"Indirect peroxidase staining using monoclonal antibodies specific for Mya arenaria neoplastic cells","created_at":"2015-10-24T05:46:08.393-07:00","url":"https://www.academia.edu/17244196/Indirect_peroxidase_staining_using_monoclonal_antibodies_specific_for_Mya_arenaria_neoplastic_cells?f_ri=55175","dom_id":"work_17244196","summary":null,"downloadable_attachments":[{"id":42285466,"asset_id":17244196,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":36774202,"first_name":"Roxanna","last_name":"Smolowitz","domain_name":"independent","page_name":"RoxannaSmolowitz","display_name":"Roxanna Smolowitz","profile_url":"https://independent.academia.edu/RoxannaSmolowitz?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":85920,"name":"Bivalvia","url":"https://www.academia.edu/Documents/in/Bivalvia?f_ri=55175","nofollow":true},{"id":146990,"name":"Invertebrate Pathology","url":"https://www.academia.edu/Documents/in/Invertebrate_Pathology?f_ri=55175","nofollow":true},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=55175"},{"id":315091,"name":"Hemocytes","url":"https://www.academia.edu/Documents/in/Hemocytes?f_ri=55175"},{"id":413115,"name":"Peroxidases","url":"https://www.academia.edu/Documents/in/Peroxidases?f_ri=55175"},{"id":437728,"name":"Isoenzymes","url":"https://www.academia.edu/Documents/in/Isoenzymes?f_ri=55175"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19433487" data-work_id="19433487" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19433487/Immobilization_of_Peroxidase_Glycoprotein_on_Gold_Electrodes_Modified_with_Mixed_Epoxy_Boronic_Acid_Monolayers">Immobilization of Peroxidase Glycoprotein on Gold Electrodes Modified with Mixed Epoxy-Boronic Acid Monolayers</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The development of bioelectronic enzyme applications requires the immobilization of active proteins onto solid or colloidal substrates such as gold. Coverage of the gold surface with alkanethiol selfassembled monolayers (SAMs) reduces... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19433487" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The development of bioelectronic enzyme applications requires the immobilization of active proteins onto solid or colloidal substrates such as gold. Coverage of the gold surface with alkanethiol selfassembled monolayers (SAMs) reduces nonspecific adsorption of proteins and also allows the incorporation onto the surface of ligands with affinity for complementary binding sites on native proteins. We present in this work a strategy for the covalent immobilization of glycosylated proteins previously adsorbed through weak, reversible interactions, on tailored SAMs. Boronic acids, which form cyclic esters with saccharides, are incorporated into SAMs to weakly adsorb the glycoprotein onto the electrode surface through their carbohydrate moiety. To prevent protein release from the electrode surface, we combine the affinity motif of boronates with the reactivity of epoxy groups to covalently link the protein to heterofunctional boronateepoxy SAMs. The principle underlying our strategy is the increased immobilization rate achieved by the weak interaction-induced proximity effect between slow reacting oxyrane groups in the SAM and nucleophilic residues from adsorbed proteins, which allows the formation of very stable covalent bonds. This approach is exemplified by the use of phenylboronates-oxyrane mixed monolayers as a reactive support and redoxenzyme horseradish peroxidase as glycoprotein for the preparation of peroxidase electrodes. Quartz crystal microbalance, atomic force microscopy, and electrochemical measurements are used to characterize these enzymatic electrodes. These epoxy-boronate functional monolayers are versatile, stable interfaces, ready to incorporate glycoproteins by incubation under mild conditions.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19433487" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="80223770b87658cac8a893688b0a3654" rel="nofollow" data-download="{"attachment_id":40617438,"asset_id":19433487,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40617438/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39703549" href="https://independent.academia.edu/IrinaGazaryan">Irina Gazaryan</a><script data-card-contents-for-user="39703549" type="text/json">{"id":39703549,"first_name":"Irina","last_name":"Gazaryan","domain_name":"independent","page_name":"IrinaGazaryan","display_name":"Irina Gazaryan","profile_url":"https://independent.academia.edu/IrinaGazaryan?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_19433487 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19433487"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19433487, container: ".js-paper-rank-work_19433487", }); });</script></li><li class="js-percentile-work_19433487 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 19433487; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_19433487"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_19433487 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="19433487"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 19433487; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=19433487]").text(description); $(".js-view-count-work_19433487").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_19433487").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="19433487"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="522" rel="nofollow" href="https://www.academia.edu/Documents/in/Thermodynamics">Thermodynamics</a>, <script data-card-contents-for-ri="522" type="text/json">{"id":522,"name":"Thermodynamics","url":"https://www.academia.edu/Documents/in/Thermodynamics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4748" rel="nofollow" href="https://www.academia.edu/Documents/in/Electrochemistry">Electrochemistry</a>, <script data-card-contents-for-ri="4748" type="text/json">{"id":4748,"name":"Electrochemistry","url":"https://www.academia.edu/Documents/in/Electrochemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="24373" rel="nofollow" href="https://www.academia.edu/Documents/in/Atomic_Force_Microscopy">Atomic Force Microscopy</a><script data-card-contents-for-ri="24373" type="text/json">{"id":24373,"name":"Atomic Force Microscopy","url":"https://www.academia.edu/Documents/in/Atomic_Force_Microscopy?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19433487]'), work: {"id":19433487,"title":"Immobilization of Peroxidase Glycoprotein on Gold Electrodes Modified with Mixed Epoxy-Boronic Acid Monolayers","created_at":"2015-12-03T13:28:37.192-08:00","url":"https://www.academia.edu/19433487/Immobilization_of_Peroxidase_Glycoprotein_on_Gold_Electrodes_Modified_with_Mixed_Epoxy_Boronic_Acid_Monolayers?f_ri=55175","dom_id":"work_19433487","summary":"The development of bioelectronic enzyme applications requires the immobilization of active proteins onto solid or colloidal substrates such as gold. Coverage of the gold surface with alkanethiol selfassembled monolayers (SAMs) reduces nonspecific adsorption of proteins and also allows the incorporation onto the surface of ligands with affinity for complementary binding sites on native proteins. We present in this work a strategy for the covalent immobilization of glycosylated proteins previously adsorbed through weak, reversible interactions, on tailored SAMs. Boronic acids, which form cyclic esters with saccharides, are incorporated into SAMs to weakly adsorb the glycoprotein onto the electrode surface through their carbohydrate moiety. To prevent protein release from the electrode surface, we combine the affinity motif of boronates with the reactivity of epoxy groups to covalently link the protein to heterofunctional boronateepoxy SAMs. The principle underlying our strategy is the increased immobilization rate achieved by the weak interaction-induced proximity effect between slow reacting oxyrane groups in the SAM and nucleophilic residues from adsorbed proteins, which allows the formation of very stable covalent bonds. This approach is exemplified by the use of phenylboronates-oxyrane mixed monolayers as a reactive support and redoxenzyme horseradish peroxidase as glycoprotein for the preparation of peroxidase electrodes. Quartz crystal microbalance, atomic force microscopy, and electrochemical measurements are used to characterize these enzymatic electrodes. These epoxy-boronate functional monolayers are versatile, stable interfaces, ready to incorporate glycoproteins by incubation under mild conditions.","downloadable_attachments":[{"id":40617438,"asset_id":19433487,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39703549,"first_name":"Irina","last_name":"Gazaryan","domain_name":"independent","page_name":"IrinaGazaryan","display_name":"Irina Gazaryan","profile_url":"https://independent.academia.edu/IrinaGazaryan?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":522,"name":"Thermodynamics","url":"https://www.academia.edu/Documents/in/Thermodynamics?f_ri=55175","nofollow":true},{"id":4748,"name":"Electrochemistry","url":"https://www.academia.edu/Documents/in/Electrochemistry?f_ri=55175","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true},{"id":24373,"name":"Atomic Force Microscopy","url":"https://www.academia.edu/Documents/in/Atomic_Force_Microscopy?f_ri=55175","nofollow":true},{"id":39752,"name":"Adsorption","url":"https://www.academia.edu/Documents/in/Adsorption?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":76736,"name":"Gold","url":"https://www.academia.edu/Documents/in/Gold?f_ri=55175"},{"id":126972,"name":"P-glycoprotein","url":"https://www.academia.edu/Documents/in/P-glycoprotein?f_ri=55175"},{"id":161176,"name":"The","url":"https://www.academia.edu/Documents/in/The?f_ri=55175"},{"id":191439,"name":"Glycoproteins","url":"https://www.academia.edu/Documents/in/Glycoproteins?f_ri=55175"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=55175"},{"id":362538,"name":"Boronic Acids","url":"https://www.academia.edu/Documents/in/Boronic_Acids?f_ri=55175"},{"id":846015,"name":"Electrodes","url":"https://www.academia.edu/Documents/in/Electrodes?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_29743000" data-work_id="29743000" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/29743000/Long_term_propylthiouracil_use_and_acute_myeloid_leukemia_A_case_report_and_review_of_the_literature">Long-term propylthiouracil use and acute myeloid leukemia: A case report and review of the literature</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/29743000" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3d6bb50b049c5a3b8347750f2dd96f8b" rel="nofollow" data-download="{"attachment_id":50200409,"asset_id":29743000,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50200409/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="52503878" href="https://independent.academia.edu/FrancescoCaracciolo4">Francesco Caracciolo</a><script data-card-contents-for-user="52503878" type="text/json">{"id":52503878,"first_name":"Francesco","last_name":"Caracciolo","domain_name":"independent","page_name":"FrancescoCaracciolo4","display_name":"Francesco Caracciolo","profile_url":"https://independent.academia.edu/FrancescoCaracciolo4?f_ri=55175","photo":"https://0.academia-photos.com/52503878/13871779/14948295/s65_francesco.caracciolo.jpg"}</script></span></span></li><li class="js-paper-rank-work_29743000 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="29743000"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 29743000, container: ".js-paper-rank-work_29743000", }); });</script></li><li class="js-percentile-work_29743000 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 29743000; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_29743000"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_29743000 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="29743000"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 29743000; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=29743000]").text(description); $(".js-view-count-work_29743000").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_29743000").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="29743000"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="59057" rel="nofollow" href="https://www.academia.edu/Documents/in/Acute_Myeloid_Leukemia">Acute Myeloid Leukemia</a>, <script data-card-contents-for-ri="59057" type="text/json">{"id":59057,"name":"Acute Myeloid Leukemia","url":"https://www.academia.edu/Documents/in/Acute_Myeloid_Leukemia?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="63886" rel="nofollow" href="https://www.academia.edu/Documents/in/Case_Report">Case Report</a>, <script data-card-contents-for-ri="63886" type="text/json">{"id":63886,"name":"Case Report","url":"https://www.academia.edu/Documents/in/Case_Report?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="413195" rel="nofollow" href="https://www.academia.edu/Documents/in/Time_Factors">Time Factors</a><script data-card-contents-for-ri="413195" type="text/json">{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=29743000]'), work: {"id":29743000,"title":"Long-term propylthiouracil use and acute myeloid leukemia: A case report and review of the literature","created_at":"2016-11-08T21:01:24.707-08:00","url":"https://www.academia.edu/29743000/Long_term_propylthiouracil_use_and_acute_myeloid_leukemia_A_case_report_and_review_of_the_literature?f_ri=55175","dom_id":"work_29743000","summary":null,"downloadable_attachments":[{"id":50200409,"asset_id":29743000,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":52503878,"first_name":"Francesco","last_name":"Caracciolo","domain_name":"independent","page_name":"FrancescoCaracciolo4","display_name":"Francesco Caracciolo","profile_url":"https://independent.academia.edu/FrancescoCaracciolo4?f_ri=55175","photo":"https://0.academia-photos.com/52503878/13871779/14948295/s65_francesco.caracciolo.jpg"}],"research_interests":[{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":59057,"name":"Acute Myeloid Leukemia","url":"https://www.academia.edu/Documents/in/Acute_Myeloid_Leukemia?f_ri=55175","nofollow":true},{"id":63886,"name":"Case Report","url":"https://www.academia.edu/Documents/in/Case_Report?f_ri=55175","nofollow":true},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=55175","nofollow":true},{"id":595154,"name":"Propylthiouracil","url":"https://www.academia.edu/Documents/in/Propylthiouracil?f_ri=55175"},{"id":710565,"name":"Annals","url":"https://www.academia.edu/Documents/in/Annals?f_ri=55175"},{"id":2374838,"name":"Hematopoietic Stem Cell Transplantation","url":"https://www.academia.edu/Documents/in/Hematopoietic_Stem_Cell_Transplantation?f_ri=55175"},{"id":2484597,"name":"Etoposide","url":"https://www.academia.edu/Documents/in/Etoposide?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3713558" data-work_id="3713558" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3713558/Caffeic_acid_inhibits_in_vitro_rooting_in_mung_bean_Vigna_radiata_L_Wilczek_hypocotyls_by_inducing_oxidative_stress">Caffeic acid inhibits in vitro rooting in mung bean [ Vigna radiata (L.) Wilczek] hypocotyls by inducing oxidative stress</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Caffeic acid (CA), which is ubiquitously present in plants, is a potent phytotoxin affecting plant growth and physiology. The aim of our study was to investigate whether CA-induced inhibition of adventitious root formation (ARF) in mung... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_3713558" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Caffeic acid (CA), which is ubiquitously present in plants, is a potent phytotoxin affecting plant growth and physiology. The aim of our study was to investigate whether CA-induced inhibition of adventitious root formation (ARF) in mung bean {Vigna radiata (L.) Wilczek [Phaseolus aureus Roxb.]} involves the induction of conventional stress responses. The effect of CA (0–1000 μM) on ARF in mung bean was determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H2O2) content, root oxidizability and changes in levels of antioxidant enzymes. Our results show that CA significantly enhanced MDA content, indicating severe lipid peroxidation, and increased H2O2 accumulation and root oxidizability in the lower rooted hypocotylar region (LRHR) of mung bean, thereby inducing oxidative stress and cellular damage. In response to CA, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, catalase and glutathione reductase, in LRHRs of mung bean. Based on these results, we conclude that CA inhibits ARF in mung bean hypocotyls by inducing ROS-generated oxidative stress and upregulating the activities of antioxidant enzymes.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3713558" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="34899620ac83aae89f1bbf35f59afc66" rel="nofollow" data-download="{"attachment_id":50166394,"asset_id":3713558,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50166394/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4543853" href="https://independent.academia.edu/DaizyBatish">Daizy Batish</a><script data-card-contents-for-user="4543853" type="text/json">{"id":4543853,"first_name":"Daizy","last_name":"Batish","domain_name":"independent","page_name":"DaizyBatish","display_name":"Daizy Batish","profile_url":"https://independent.academia.edu/DaizyBatish?f_ri=55175","photo":"https://0.academia-photos.com/4543853/1880669/2232035/s65_daizy.batish.jpg"}</script></span></span></li><li class="js-paper-rank-work_3713558 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="3713558"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 3713558, container: ".js-paper-rank-work_3713558", }); });</script></li><li class="js-percentile-work_3713558 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 3713558; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_3713558"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_3713558 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="3713558"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 3713558; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=3713558]").text(description); $(".js-view-count-work_3713558").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_3713558").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="3713558"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="98707" rel="nofollow" href="https://www.academia.edu/Documents/in/Stress_response">Stress response</a>, <script data-card-contents-for-ri="98707" type="text/json">{"id":98707,"name":"Stress response","url":"https://www.academia.edu/Documents/in/Stress_response?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="127006" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_growth">Plant growth</a><script data-card-contents-for-ri="127006" type="text/json">{"id":127006,"name":"Plant growth","url":"https://www.academia.edu/Documents/in/Plant_growth?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=3713558]'), work: {"id":3713558,"title":"Caffeic acid inhibits in vitro rooting in mung bean [ Vigna radiata (L.) Wilczek] hypocotyls by inducing oxidative stress","created_at":"2013-06-14T15:39:44.370-07:00","url":"https://www.academia.edu/3713558/Caffeic_acid_inhibits_in_vitro_rooting_in_mung_bean_Vigna_radiata_L_Wilczek_hypocotyls_by_inducing_oxidative_stress?f_ri=55175","dom_id":"work_3713558","summary":"Caffeic acid (CA), which is ubiquitously present in plants, is a potent phytotoxin affecting plant growth and physiology. The aim of our study was to investigate whether CA-induced inhibition of adventitious root formation (ARF) in mung bean {Vigna radiata (L.) Wilczek [Phaseolus aureus Roxb.]} involves the induction of conventional stress responses. The effect of CA (0–1000 μM) on ARF in mung bean was determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H2O2) content, root oxidizability and changes in levels of antioxidant enzymes. Our results show that CA significantly enhanced MDA content, indicating severe lipid peroxidation, and increased H2O2 accumulation and root oxidizability in the lower rooted hypocotylar region (LRHR) of mung bean, thereby inducing oxidative stress and cellular damage. In response to CA, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, catalase and glutathione reductase, in LRHRs of mung bean. Based on these results, we conclude that CA inhibits ARF in mung bean hypocotyls by inducing ROS-generated oxidative stress and upregulating the activities of antioxidant enzymes.","downloadable_attachments":[{"id":50166394,"asset_id":3713558,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4543853,"first_name":"Daizy","last_name":"Batish","domain_name":"independent","page_name":"DaizyBatish","display_name":"Daizy Batish","profile_url":"https://independent.academia.edu/DaizyBatish?f_ri=55175","photo":"https://0.academia-photos.com/4543853/1880669/2232035/s65_daizy.batish.jpg"}],"research_interests":[{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":98707,"name":"Stress response","url":"https://www.academia.edu/Documents/in/Stress_response?f_ri=55175","nofollow":true},{"id":127006,"name":"Plant growth","url":"https://www.academia.edu/Documents/in/Plant_growth?f_ri=55175","nofollow":true},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=55175"},{"id":196241,"name":"Plant Growth Regulation","url":"https://www.academia.edu/Documents/in/Plant_Growth_Regulation?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide?f_ri=55175"},{"id":295257,"name":"Antioxidant enzyme","url":"https://www.academia.edu/Documents/in/Antioxidant_enzyme?f_ri=55175"},{"id":427871,"name":"Oxidative Damage","url":"https://www.academia.edu/Documents/in/Oxidative_Damage?f_ri=55175"},{"id":446141,"name":"Vigna Radiata","url":"https://www.academia.edu/Documents/in/Vigna_Radiata?f_ri=55175"},{"id":618409,"name":"Mung Bean","url":"https://www.academia.edu/Documents/in/Mung_Bean?f_ri=55175"},{"id":1011463,"name":"Glutathione Reductase","url":"https://www.academia.edu/Documents/in/Glutathione_Reductase?f_ri=55175"},{"id":1446339,"name":"Malondialdehyde","url":"https://www.academia.edu/Documents/in/Malondialdehyde?f_ri=55175"},{"id":2278598,"name":"Ascorbate Peroxidase","url":"https://www.academia.edu/Documents/in/Ascorbate_Peroxidase?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6935134" data-work_id="6935134" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6935134/Effect_of_Pb_toxicity_on_leaf_growth_physiology_and_ultrastructure_in_the_two_ecotypes_of_Elsholtzia_argyi">Effect of Pb toxicity on leaf growth, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Hydroponics experiments were conducted to study the effects of Pb on mined ecotype (ME) and non-mined ecotype (NME) of Elsholtzia argyi from Pb/Cu mining and the non-contaminated agricultural areas, respectively. The results showed that... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6935134" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Hydroponics experiments were conducted to study the effects of Pb on mined ecotype (ME) and non-mined ecotype (NME) of Elsholtzia argyi from Pb/Cu mining and the non-contaminated agricultural areas, respectively. The results showed that at 200 M Pb treatment, although concentrations of Pb in leaves and stem of the ME were 2.6 and 4.5 times higher than those of the NME, these plants exhibited higher tolerance to excessive levels of Pb in the growth medium. In both the ecotypes, Pb caused inhibition of leaf growth and photosynthesis, and induced the membrane damage which was more obvious in the NME. Pb treatment decreased the activities of superoxide dismutase (SOD) and guaiacol peroxidase (G-POD) while activity of catalase (CAT) and levels of total soluble proteins (TSP), ascorbic acid (AsA) and glutathione (GSH) were significantly induced after Pb exposure, however, increase was sharp in the ME plants. Leaf ultrastructural analysis of the spongy mesophyll cells revealed that the excessive Pb concentrations caused adverse effects on chloroplast ultrastructure of both ecotypes whereby internal damage was more severe in NME. The higher tolerance to Pb displayed by ME is mainly attributed to maintenance of its leaf growth and physiology, induction of GSH and integrity of cell organelles especially chloroplast ultrastructure.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6935134" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0084c45b40e38e3ad34c4d7b93cd6d4e" rel="nofollow" data-download="{"attachment_id":48661442,"asset_id":6935134,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48661442/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="11705801" href="https://comsats.academia.edu/QaisarMahmood">Qaisar Mahmood</a><script data-card-contents-for-user="11705801" type="text/json">{"id":11705801,"first_name":"Qaisar","last_name":"Mahmood","domain_name":"comsats","page_name":"QaisarMahmood","display_name":"Qaisar Mahmood","profile_url":"https://comsats.academia.edu/QaisarMahmood?f_ri=55175","photo":"https://0.academia-photos.com/11705801/3375704/3972227/s65_qaisar.mahmood.jpg"}</script></span></span></li><li class="js-paper-rank-work_6935134 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6935134"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6935134, container: ".js-paper-rank-work_6935134", }); });</script></li><li class="js-percentile-work_6935134 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 6935134; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_6935134"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_6935134 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="6935134"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 6935134; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=6935134]").text(description); $(".js-view-count-work_6935134").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6935134").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6935134"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" rel="nofollow" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5345" rel="nofollow" href="https://www.academia.edu/Documents/in/Photosynthesis">Photosynthesis</a>, <script data-card-contents-for-ri="5345" type="text/json">{"id":5345,"name":"Photosynthesis","url":"https://www.academia.edu/Documents/in/Photosynthesis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12108" rel="nofollow" href="https://www.academia.edu/Documents/in/China">China</a>, <script data-card-contents-for-ri="12108" type="text/json">{"id":12108,"name":"China","url":"https://www.academia.edu/Documents/in/China?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14076" rel="nofollow" href="https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy">Transmission Electron Microscopy</a><script data-card-contents-for-ri="14076" type="text/json">{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6935134]'), work: {"id":6935134,"title":"Effect of Pb toxicity on leaf growth, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi","created_at":"2014-05-01T19:26:46.267-07:00","url":"https://www.academia.edu/6935134/Effect_of_Pb_toxicity_on_leaf_growth_physiology_and_ultrastructure_in_the_two_ecotypes_of_Elsholtzia_argyi?f_ri=55175","dom_id":"work_6935134","summary":"Hydroponics experiments were conducted to study the effects of Pb on mined ecotype (ME) and non-mined ecotype (NME) of Elsholtzia argyi from Pb/Cu mining and the non-contaminated agricultural areas, respectively. The results showed that at 200 M Pb treatment, although concentrations of Pb in leaves and stem of the ME were 2.6 and 4.5 times higher than those of the NME, these plants exhibited higher tolerance to excessive levels of Pb in the growth medium. In both the ecotypes, Pb caused inhibition of leaf growth and photosynthesis, and induced the membrane damage which was more obvious in the NME. Pb treatment decreased the activities of superoxide dismutase (SOD) and guaiacol peroxidase (G-POD) while activity of catalase (CAT) and levels of total soluble proteins (TSP), ascorbic acid (AsA) and glutathione (GSH) were significantly induced after Pb exposure, however, increase was sharp in the ME plants. Leaf ultrastructural analysis of the spongy mesophyll cells revealed that the excessive Pb concentrations caused adverse effects on chloroplast ultrastructure of both ecotypes whereby internal damage was more severe in NME. The higher tolerance to Pb displayed by ME is mainly attributed to maintenance of its leaf growth and physiology, induction of GSH and integrity of cell organelles especially chloroplast ultrastructure.","downloadable_attachments":[{"id":48661442,"asset_id":6935134,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":11705801,"first_name":"Qaisar","last_name":"Mahmood","domain_name":"comsats","page_name":"QaisarMahmood","display_name":"Qaisar Mahmood","profile_url":"https://comsats.academia.edu/QaisarMahmood?f_ri=55175","photo":"https://0.academia-photos.com/11705801/3375704/3972227/s65_qaisar.mahmood.jpg"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true},{"id":5345,"name":"Photosynthesis","url":"https://www.academia.edu/Documents/in/Photosynthesis?f_ri=55175","nofollow":true},{"id":12108,"name":"China","url":"https://www.academia.edu/Documents/in/China?f_ri=55175","nofollow":true},{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":59404,"name":"Lamiaceae","url":"https://www.academia.edu/Documents/in/Lamiaceae?f_ri=55175"},{"id":116579,"name":"Lead","url":"https://www.academia.edu/Documents/in/Lead?f_ri=55175"},{"id":118450,"name":"Glutathione","url":"https://www.academia.edu/Documents/in/Glutathione?f_ri=55175"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=55175"},{"id":149625,"name":"Hazardous Materials","url":"https://www.academia.edu/Documents/in/Hazardous_Materials?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":233372,"name":"Lipid peroxidation","url":"https://www.academia.edu/Documents/in/Lipid_peroxidation?f_ri=55175"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide?f_ri=55175"},{"id":283613,"name":"Leaf Growth","url":"https://www.academia.edu/Documents/in/Leaf_Growth?f_ri=55175"},{"id":352757,"name":"Ascorbic Acid","url":"https://www.academia.edu/Documents/in/Ascorbic_Acid?f_ri=55175"},{"id":394388,"name":"Hazardous","url":"https://www.academia.edu/Documents/in/Hazardous?f_ri=55175"},{"id":1181939,"name":"PLANT PROTEINS","url":"https://www.academia.edu/Documents/in/PLANT_PROTEINS?f_ri=55175"},{"id":1592853,"name":"Drug Tolerance","url":"https://www.academia.edu/Documents/in/Drug_Tolerance?f_ri=55175"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27394198" data-work_id="27394198" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27394198/Modulating_effect_of_ginger_extract_on_rats_with_ulcerative_colitis">Modulating effect of ginger extract on rats with ulcerative colitis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Ethnopharmacological relevance: Ginger rhizomes are used traditionally for management of different gastrointestinal disturbances. Several studies proved that the rhizome possesses diverse biological activities such as cytotoxic,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_27394198" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Ethnopharmacological relevance: Ginger rhizomes are used traditionally for management of different gastrointestinal disturbances. Several studies proved that the rhizome possesses diverse biological activities such as cytotoxic, antioxidant, and anti-inflammatory effects. Recently, interest in ginger for treatment of chronic inflammatory conditions has been renewed. Aim of the study: The purpose of the present study is to evaluate the potential role of ginger extract [GE] in modulating the extent and severity of ulcerative colitis (UC), a chronically recurrent inflammatory bowel disease of unknown origin. Materials and methods: Male Wistar rats received 3 different doses of GE, sulfasalazine, or vehicle for 3 consecutive days before induction of UC by intra-rectal acetic acid administration, and continued further for 7 days after the induction. The colonic mucosal injury was assessed by macroscopic scoring, and histological examination. Furthermore, the mucosal content of malondialdehyde (MDA), protein carbonyl (PCO), and reduced glutathione (GSH) with the catalase (CAT) and superoxide dismutase (SOD) activity, were appraised as parameters of the redox state. Acute inflammatory response was determined by measuring myeloperoxidase (MPO), tumor necrosis factor (TNF-␣), and prostaglandin E 2 (PGE 2 ). Results: All parameters were altered in ulcerated rats, and improved in animals receiving GE, an effect that was comparable to that of the standard sulfasalazine, especially at the highest dose level. Colonic mucosal injury parallels with the histological and biochemical evaluations. Conclusions: Results showed a valuable effect of ginger extract against acetic acid-induced ulcerative colitis possibly by its antioxidant and anti-inflammatory properties.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27394198" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="29b52066dac4e2fcaf454ad7c6f289ad" rel="nofollow" data-download="{"attachment_id":47649006,"asset_id":27394198,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47649006/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="51513653" href="https://independent.academia.edu/HananSalahdinElabhar">Hanan Salahdin Elabhar</a><script data-card-contents-for-user="51513653" type="text/json">{"id":51513653,"first_name":"Hanan","last_name":"Salahdin Elabhar","domain_name":"independent","page_name":"HananSalahdinElabhar","display_name":"Hanan Salahdin Elabhar","profile_url":"https://independent.academia.edu/HananSalahdinElabhar?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_27394198 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="27394198"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 27394198, container: ".js-paper-rank-work_27394198", }); });</script></li><li class="js-percentile-work_27394198 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27394198; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_27394198"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_27394198 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="27394198"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27394198; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27394198]").text(description); $(".js-view-count-work_27394198").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_27394198").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="27394198"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="4083" rel="nofollow" href="https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine">Complementary and Alternative Medicine</a>, <script data-card-contents-for-ri="4083" type="text/json">{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14032" rel="nofollow" href="https://www.academia.edu/Documents/in/Ethnopharmacology">Ethnopharmacology</a>, <script data-card-contents-for-ri="14032" type="text/json">{"id":14032,"name":"Ethnopharmacology","url":"https://www.academia.edu/Documents/in/Ethnopharmacology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14080" rel="nofollow" href="https://www.academia.edu/Documents/in/Pharmacognosy">Pharmacognosy</a><script data-card-contents-for-ri="14080" type="text/json">{"id":14080,"name":"Pharmacognosy","url":"https://www.academia.edu/Documents/in/Pharmacognosy?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=27394198]'), work: {"id":27394198,"title":"Modulating effect of ginger extract on rats with ulcerative colitis","created_at":"2016-07-30T08:53:43.763-07:00","url":"https://www.academia.edu/27394198/Modulating_effect_of_ginger_extract_on_rats_with_ulcerative_colitis?f_ri=55175","dom_id":"work_27394198","summary":"Ethnopharmacological relevance: Ginger rhizomes are used traditionally for management of different gastrointestinal disturbances. Several studies proved that the rhizome possesses diverse biological activities such as cytotoxic, antioxidant, and anti-inflammatory effects. Recently, interest in ginger for treatment of chronic inflammatory conditions has been renewed. Aim of the study: The purpose of the present study is to evaluate the potential role of ginger extract [GE] in modulating the extent and severity of ulcerative colitis (UC), a chronically recurrent inflammatory bowel disease of unknown origin. Materials and methods: Male Wistar rats received 3 different doses of GE, sulfasalazine, or vehicle for 3 consecutive days before induction of UC by intra-rectal acetic acid administration, and continued further for 7 days after the induction. The colonic mucosal injury was assessed by macroscopic scoring, and histological examination. Furthermore, the mucosal content of malondialdehyde (MDA), protein carbonyl (PCO), and reduced glutathione (GSH) with the catalase (CAT) and superoxide dismutase (SOD) activity, were appraised as parameters of the redox state. Acute inflammatory response was determined by measuring myeloperoxidase (MPO), tumor necrosis factor (TNF-␣), and prostaglandin E 2 (PGE 2 ). Results: All parameters were altered in ulcerated rats, and improved in animals receiving GE, an effect that was comparable to that of the standard sulfasalazine, especially at the highest dose level. Colonic mucosal injury parallels with the histological and biochemical evaluations. Conclusions: Results showed a valuable effect of ginger extract against acetic acid-induced ulcerative colitis possibly by its antioxidant and anti-inflammatory properties.","downloadable_attachments":[{"id":47649006,"asset_id":27394198,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":51513653,"first_name":"Hanan","last_name":"Salahdin Elabhar","domain_name":"independent","page_name":"HananSalahdinElabhar","display_name":"Hanan Salahdin Elabhar","profile_url":"https://independent.academia.edu/HananSalahdinElabhar?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true},{"id":14032,"name":"Ethnopharmacology","url":"https://www.academia.edu/Documents/in/Ethnopharmacology?f_ri=55175","nofollow":true},{"id":14080,"name":"Pharmacognosy","url":"https://www.academia.edu/Documents/in/Pharmacognosy?f_ri=55175","nofollow":true},{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=55175"},{"id":52027,"name":"Phytotherapy","url":"https://www.academia.edu/Documents/in/Phytotherapy?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":83691,"name":"Ulcerative colitis","url":"https://www.academia.edu/Documents/in/Ulcerative_colitis?f_ri=55175"},{"id":204350,"name":"Anti-inflammatory agents","url":"https://www.academia.edu/Documents/in/Anti-inflammatory_agents?f_ri=55175"},{"id":233372,"name":"Lipid peroxidation","url":"https://www.academia.edu/Documents/in/Lipid_peroxidation?f_ri=55175"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":417494,"name":"Rat","url":"https://www.academia.edu/Documents/in/Rat?f_ri=55175"},{"id":564879,"name":"Wistar Rats","url":"https://www.academia.edu/Documents/in/Wistar_Rats?f_ri=55175"},{"id":598971,"name":"Ginger","url":"https://www.academia.edu/Documents/in/Ginger?f_ri=55175"},{"id":783432,"name":"Biological activity","url":"https://www.academia.edu/Documents/in/Biological_activity?f_ri=55175"},{"id":789968,"name":"Extract","url":"https://www.academia.edu/Documents/in/Extract?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_25978763 coauthored" data-work_id="25978763" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/25978763/Analysis_and_expression_of_the_class_III_peroxidase_large_gene_family_in_Arabidopsis_thaliana">Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Higher plants possess a large set of the classical guaiacol peroxidases (class III peroxidases, E.C. 1.11.1.7). These enzymes have been implicated in a wide array of physiological processes such as H 2 O 2 detoxification, auxin catabolism... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_25978763" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Higher plants possess a large set of the classical guaiacol peroxidases (class III peroxidases, E.C. 1.11.1.7). These enzymes have been implicated in a wide array of physiological processes such as H 2 O 2 detoxification, auxin catabolism and lignin biosynthesis and stress response (wounding, pathogen attack, etc.). During the last 10 years, molecular cloning has allowed the isolation and characterization of several genes encoding peroxidases in plants. The achievement of the large scale Arabidopsis genome sequencing, combined with the DNA complementary to RNA (cDNA) expressed sequence tags projects, provided the opportunity to draw up the first comprehensive list of peroxidases in a plant. By screening the available databases, we have identified 73 peroxidase genes throughout the Arabidopsis genome. The evolution of the peroxidase multigene family has been investigated by analyzing the gene structure (intron/exon) in correlation with the phylogenetic relationships between the isoperoxidases. An evolutionary pattern of extensive gene duplications can be inferred and is discussed. Using a cDNA array procedure, the expression pattern of 23 peroxidases was established in the different organs of the plant. All the tested peroxidases were expressed at various levels in roots, while several were also detected in stems, leaves and flowers. The specific functions of these genes remain to be determined. q</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/25978763" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="08f01ee7ce27fe45a5b629a311daae8b" rel="nofollow" data-download="{"attachment_id":46329719,"asset_id":25978763,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46329719/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="49791559" href="https://independent.academia.edu/HubertGreppin">Hubert Greppin</a><script data-card-contents-for-user="49791559" type="text/json">{"id":49791559,"first_name":"Hubert","last_name":"Greppin","domain_name":"independent","page_name":"HubertGreppin","display_name":"Hubert Greppin","profile_url":"https://independent.academia.edu/HubertGreppin?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-25978763">+1</span><div class="hidden js-additional-users-25978763"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/SimonPatrice">Patrice Simon</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-25978763'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-25978763').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_25978763 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="25978763"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 25978763, container: ".js-paper-rank-work_25978763", }); });</script></li><li class="js-percentile-work_25978763 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 25978763; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_25978763"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_25978763 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="25978763"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 25978763; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=25978763]").text(description); $(".js-view-count-work_25978763").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_25978763").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="25978763"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="156" rel="nofollow" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="41553" rel="nofollow" href="https://www.academia.edu/Documents/in/Arabidopsis_thaliana">Arabidopsis thaliana</a>, <script data-card-contents-for-ri="41553" type="text/json">{"id":41553,"name":"Arabidopsis thaliana","url":"https://www.academia.edu/Documents/in/Arabidopsis_thaliana?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="54433" rel="nofollow" href="https://www.academia.edu/Documents/in/Phylogeny">Phylogeny</a>, <script data-card-contents-for-ri="54433" type="text/json">{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=25978763]'), work: {"id":25978763,"title":"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana","created_at":"2016-06-07T22:29:33.799-07:00","url":"https://www.academia.edu/25978763/Analysis_and_expression_of_the_class_III_peroxidase_large_gene_family_in_Arabidopsis_thaliana?f_ri=55175","dom_id":"work_25978763","summary":"Higher plants possess a large set of the classical guaiacol peroxidases (class III peroxidases, E.C. 1.11.1.7). These enzymes have been implicated in a wide array of physiological processes such as H 2 O 2 detoxification, auxin catabolism and lignin biosynthesis and stress response (wounding, pathogen attack, etc.). During the last 10 years, molecular cloning has allowed the isolation and characterization of several genes encoding peroxidases in plants. The achievement of the large scale Arabidopsis genome sequencing, combined with the DNA complementary to RNA (cDNA) expressed sequence tags projects, provided the opportunity to draw up the first comprehensive list of peroxidases in a plant. By screening the available databases, we have identified 73 peroxidase genes throughout the Arabidopsis genome. The evolution of the peroxidase multigene family has been investigated by analyzing the gene structure (intron/exon) in correlation with the phylogenetic relationships between the isoperoxidases. An evolutionary pattern of extensive gene duplications can be inferred and is discussed. Using a cDNA array procedure, the expression pattern of 23 peroxidases was established in the different organs of the plant. All the tested peroxidases were expressed at various levels in roots, while several were also detected in stems, leaves and flowers. The specific functions of these genes remain to be determined. q","downloadable_attachments":[{"id":46329719,"asset_id":25978763,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":49791559,"first_name":"Hubert","last_name":"Greppin","domain_name":"independent","page_name":"HubertGreppin","display_name":"Hubert Greppin","profile_url":"https://independent.academia.edu/HubertGreppin?f_ri=55175","photo":"/images/s65_no_pic.png"},{"id":51416758,"first_name":"Patrice","last_name":"Simon","domain_name":"independent","page_name":"SimonPatrice","display_name":"Patrice Simon","profile_url":"https://independent.academia.edu/SimonPatrice?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=55175","nofollow":true},{"id":41553,"name":"Arabidopsis thaliana","url":"https://www.academia.edu/Documents/in/Arabidopsis_thaliana?f_ri=55175","nofollow":true},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":57178,"name":"Plant Genome Project","url":"https://www.academia.edu/Documents/in/Plant_Genome_Project?f_ri=55175"},{"id":98707,"name":"Stress response","url":"https://www.academia.edu/Documents/in/Stress_response?f_ri=55175"},{"id":155096,"name":"Detoxification","url":"https://www.academia.edu/Documents/in/Detoxification?f_ri=55175"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene?f_ri=55175"},{"id":186941,"name":"Gene Duplication","url":"https://www.academia.edu/Documents/in/Gene_Duplication?f_ri=55175"},{"id":202410,"name":"Green Fluorescent Protein","url":"https://www.academia.edu/Documents/in/Green_Fluorescent_Protein?f_ri=55175"},{"id":202413,"name":"Arabidopsis","url":"https://www.academia.edu/Documents/in/Arabidopsis?f_ri=55175"},{"id":224866,"name":"Gene Structure","url":"https://www.academia.edu/Documents/in/Gene_Structure?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning?f_ri=55175"},{"id":394364,"name":"Auxin","url":"https://www.academia.edu/Documents/in/Auxin?f_ri=55175"},{"id":413115,"name":"Peroxidases","url":"https://www.academia.edu/Documents/in/Peroxidases?f_ri=55175"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation?f_ri=55175"},{"id":732028,"name":"Genome sequence","url":"https://www.academia.edu/Documents/in/Genome_sequence?f_ri=55175"},{"id":758278,"name":"Large Scale","url":"https://www.academia.edu/Documents/in/Large_Scale?f_ri=55175"},{"id":999803,"name":"Gene Family","url":"https://www.academia.edu/Documents/in/Gene_Family?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13267079 coauthored" data-work_id="13267079" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13267079/Flow_injection_analysis_amperometric_determination_of_ascorbic_and_uric_acids_in_urine_using_arrays_of_gold_microelectrodes_modified_by_electrodeposition_of_palladium">Flow injection analysis-amperometric determination of ascorbic and uric acids in urine using arrays of gold microelectrodes modified by electrodeposition of palladium</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Uric acid (UA) and ascorbic acid (AA) present in urine were rapidly determined by the amperometric method in association with flow injection analysis. An array of gold microelectrodes modified by electrochemical deposition of palladium... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13267079" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Uric acid (UA) and ascorbic acid (AA) present in urine were rapidly determined by the amperometric method in association with flow injection analysis. An array of gold microelectrodes modified by electrochemical deposition of palladium was employed as the working electrode. Uric and ascorbic acids were quantified in urine using amperometric differential measurements at +0.75 and +0.55 V, respectively. This method is based on three steps involving the flow injection of: (1) the sample spiked with a standard solution, (2) the pure sample, and (3) the enzymatically treated sample. The enzymatic treatment was carried out with ascorbate oxidase, uricase, and peroxidase at pH 7. The calibration curves for freshly prepared ascorbic and uric acid standards were very linear in the concentration ranges of 0.44-2.64 mg l −1 (AA) and of 0.34-1.68 mg l −1 (UA) with a relative standard deviation (RSD) <1 %. For uric acid, the real sample analyses where compared with the classical spectrophotometric method, showing deviations between 3.1 and 8.6% (n = 9). : S 0 0 0 3 -2 6 7 0 ( 9 9 ) 0 0 6 7 4 -1</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13267079" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bcadcfb2c1388cd827c09383fb0f1fe3" rel="nofollow" data-download="{"attachment_id":45530668,"asset_id":13267079,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45530668/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32523660" href="https://usp-br.academia.edu/ClaudimirLago">Claudimir Lago</a><script data-card-contents-for-user="32523660" type="text/json">{"id":32523660,"first_name":"Claudimir","last_name":"Lago","domain_name":"usp-br","page_name":"ClaudimirLago","display_name":"Claudimir Lago","profile_url":"https://usp-br.academia.edu/ClaudimirLago?f_ri=55175","photo":"https://0.academia-photos.com/32523660/75963424/64481328/s65_claudimir.lago.jpeg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13267079">+1</span><div class="hidden js-additional-users-13267079"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/M%C3%A1rcioAugelli">Márcio Augelli</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13267079'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13267079').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_13267079 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13267079"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13267079, container: ".js-paper-rank-work_13267079", }); });</script></li><li class="js-percentile-work_13267079 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13267079; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_13267079"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_13267079 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="13267079"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13267079; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13267079]").text(description); $(".js-view-count-work_13267079").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_13267079").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="13267079"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="524" rel="nofollow" href="https://www.academia.edu/Documents/in/Analytical_Chemistry">Analytical Chemistry</a>, <script data-card-contents-for-ri="524" type="text/json">{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="70044" rel="nofollow" href="https://www.academia.edu/Documents/in/Flow_Injection_Analysis">Flow Injection Analysis</a>, <script data-card-contents-for-ri="70044" type="text/json">{"id":70044,"name":"Flow Injection Analysis","url":"https://www.academia.edu/Documents/in/Flow_Injection_Analysis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="309088" rel="nofollow" href="https://www.academia.edu/Documents/in/Uric_Acid">Uric Acid</a><script data-card-contents-for-ri="309088" type="text/json">{"id":309088,"name":"Uric Acid","url":"https://www.academia.edu/Documents/in/Uric_Acid?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13267079]'), work: {"id":13267079,"title":"Flow injection analysis-amperometric determination of ascorbic and uric acids in urine using arrays of gold microelectrodes modified by electrodeposition of palladium","created_at":"2015-06-25T01:45:41.020-07:00","url":"https://www.academia.edu/13267079/Flow_injection_analysis_amperometric_determination_of_ascorbic_and_uric_acids_in_urine_using_arrays_of_gold_microelectrodes_modified_by_electrodeposition_of_palladium?f_ri=55175","dom_id":"work_13267079","summary":"Uric acid (UA) and ascorbic acid (AA) present in urine were rapidly determined by the amperometric method in association with flow injection analysis. An array of gold microelectrodes modified by electrochemical deposition of palladium was employed as the working electrode. Uric and ascorbic acids were quantified in urine using amperometric differential measurements at +0.75 and +0.55 V, respectively. This method is based on three steps involving the flow injection of: (1) the sample spiked with a standard solution, (2) the pure sample, and (3) the enzymatically treated sample. The enzymatic treatment was carried out with ascorbate oxidase, uricase, and peroxidase at pH 7. The calibration curves for freshly prepared ascorbic and uric acid standards were very linear in the concentration ranges of 0.44-2.64 mg l −1 (AA) and of 0.34-1.68 mg l −1 (UA) with a relative standard deviation (RSD) \u003c1 %. For uric acid, the real sample analyses where compared with the classical spectrophotometric method, showing deviations between 3.1 and 8.6% (n = 9). : S 0 0 0 3 -2 6 7 0 ( 9 9 ) 0 0 6 7 4 -1","downloadable_attachments":[{"id":45530668,"asset_id":13267079,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32523660,"first_name":"Claudimir","last_name":"Lago","domain_name":"usp-br","page_name":"ClaudimirLago","display_name":"Claudimir Lago","profile_url":"https://usp-br.academia.edu/ClaudimirLago?f_ri=55175","photo":"https://0.academia-photos.com/32523660/75963424/64481328/s65_claudimir.lago.jpeg"},{"id":32570906,"first_name":"Márcio","last_name":"Augelli","domain_name":"independent","page_name":"MárcioAugelli","display_name":"Márcio Augelli","profile_url":"https://independent.academia.edu/M%C3%A1rcioAugelli?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":70044,"name":"Flow Injection Analysis","url":"https://www.academia.edu/Documents/in/Flow_Injection_Analysis?f_ri=55175","nofollow":true},{"id":309088,"name":"Uric Acid","url":"https://www.academia.edu/Documents/in/Uric_Acid?f_ri=55175","nofollow":true},{"id":352757,"name":"Ascorbic Acid","url":"https://www.academia.edu/Documents/in/Ascorbic_Acid?f_ri=55175"},{"id":724373,"name":"Electrochemical Deposition","url":"https://www.academia.edu/Documents/in/Electrochemical_Deposition?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_25042032" data-work_id="25042032" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/25042032/The_Inhibitory_Effects_of_Coumarin_on_the_Germination_of_Durum_Wheat_Triticum_turgidum_ssp_durum_cv_Simeto_Seeds">The Inhibitory Effects of Coumarin on the Germination of Durum Wheat (Triticum turgidum ssp. durum, cv. Simeto) Seeds</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The event chain leading to germination blockage in durum wheat (Triticum turgidum ssp. durum Desf.) seeds exposed to the allelochemical coumarin (2H-chromen-2-one) was studied. The physiological and biochemical aspects thought to be... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_25042032" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The event chain leading to germination blockage in durum wheat (Triticum turgidum ssp. durum Desf.) seeds exposed to the allelochemical coumarin (2H-chromen-2-one) was studied. The physiological and biochemical aspects thought to be critical for a successful seed germination were measured. At concentrations above 200 2M, coumarin inhibited seed germination in a concentration-dependent manner. Inhibition occurred early during seed imbibition (phase I), was rapid, and irreversible. During phase I, coumarin inhibited water uptake, electrolyte retention capacity, and O 2 consumption. Later on, coumarin delayed the reactivation of peroxidases, enhanced the activity of superoxide dismutase, decreased the activities of selected marker enzymes for metabolic resumption, and repressed the transcription of molecular chaperons involved in secretory pathways. Insufficient and/or late seed rehydration caused by coumarin could have delayed membrane stabilization or decreased respiratory O 2 consumption, both of which are conducive to an overproduction of reactive O 2 species. Being unbalanced by an adequate upsurge of antioxidant defense systems, the resulting oxidative stress might have ultimately interfered with the germination program.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/25042032" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e57de8f2799f5dc527d641004fe5f041" rel="nofollow" data-download="{"attachment_id":45365382,"asset_id":25042032,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45365382/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="48156056" href="https://independent.academia.edu/AnnaRitaPaolacci">Anna Rita Paolacci</a><script data-card-contents-for-user="48156056" type="text/json">{"id":48156056,"first_name":"Anna Rita","last_name":"Paolacci","domain_name":"independent","page_name":"AnnaRitaPaolacci","display_name":"Anna Rita Paolacci","profile_url":"https://independent.academia.edu/AnnaRitaPaolacci?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_25042032 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="25042032"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 25042032, container: ".js-paper-rank-work_25042032", }); });</script></li><li class="js-percentile-work_25042032 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 25042032; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_25042032"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_25042032 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="25042032"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 25042032; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=25042032]").text(description); $(".js-view-count-work_25042032").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_25042032").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="25042032"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2215" rel="nofollow" href="https://www.academia.edu/Documents/in/Water">Water</a>, <script data-card-contents-for-ri="2215" type="text/json">{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4497" rel="nofollow" href="https://www.academia.edu/Documents/in/Chemical_Ecology">Chemical Ecology</a>, <script data-card-contents-for-ri="4497" type="text/json">{"id":4497,"name":"Chemical Ecology","url":"https://www.academia.edu/Documents/in/Chemical_Ecology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="15608" rel="nofollow" href="https://www.academia.edu/Documents/in/Seed_germination">Seed germination</a>, <script data-card-contents-for-ri="15608" type="text/json">{"id":15608,"name":"Seed germination","url":"https://www.academia.edu/Documents/in/Seed_germination?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=25042032]'), work: {"id":25042032,"title":"The Inhibitory Effects of Coumarin on the Germination of Durum Wheat (Triticum turgidum ssp. durum, cv. Simeto) Seeds","created_at":"2016-05-04T23:00:07.426-07:00","url":"https://www.academia.edu/25042032/The_Inhibitory_Effects_of_Coumarin_on_the_Germination_of_Durum_Wheat_Triticum_turgidum_ssp_durum_cv_Simeto_Seeds?f_ri=55175","dom_id":"work_25042032","summary":"The event chain leading to germination blockage in durum wheat (Triticum turgidum ssp. durum Desf.) seeds exposed to the allelochemical coumarin (2H-chromen-2-one) was studied. The physiological and biochemical aspects thought to be critical for a successful seed germination were measured. At concentrations above 200 2M, coumarin inhibited seed germination in a concentration-dependent manner. Inhibition occurred early during seed imbibition (phase I), was rapid, and irreversible. During phase I, coumarin inhibited water uptake, electrolyte retention capacity, and O 2 consumption. Later on, coumarin delayed the reactivation of peroxidases, enhanced the activity of superoxide dismutase, decreased the activities of selected marker enzymes for metabolic resumption, and repressed the transcription of molecular chaperons involved in secretory pathways. Insufficient and/or late seed rehydration caused by coumarin could have delayed membrane stabilization or decreased respiratory O 2 consumption, both of which are conducive to an overproduction of reactive O 2 species. Being unbalanced by an adequate upsurge of antioxidant defense systems, the resulting oxidative stress might have ultimately interfered with the germination program.","downloadable_attachments":[{"id":45365382,"asset_id":25042032,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":48156056,"first_name":"Anna Rita","last_name":"Paolacci","domain_name":"independent","page_name":"AnnaRitaPaolacci","display_name":"Anna Rita Paolacci","profile_url":"https://independent.academia.edu/AnnaRitaPaolacci?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=55175","nofollow":true},{"id":4497,"name":"Chemical Ecology","url":"https://www.academia.edu/Documents/in/Chemical_Ecology?f_ri=55175","nofollow":true},{"id":15608,"name":"Seed germination","url":"https://www.academia.edu/Documents/in/Seed_germination?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=55175"},{"id":87023,"name":"Secretory Pathway","url":"https://www.academia.edu/Documents/in/Secretory_Pathway?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=55175"},{"id":315117,"name":"Germination","url":"https://www.academia.edu/Documents/in/Germination?f_ri=55175"},{"id":325790,"name":"Coumarins","url":"https://www.academia.edu/Documents/in/Coumarins?f_ri=55175"},{"id":380825,"name":"Oxygen","url":"https://www.academia.edu/Documents/in/Oxygen?f_ri=55175"},{"id":386438,"name":"Seeds","url":"https://www.academia.edu/Documents/in/Seeds?f_ri=55175"},{"id":396752,"name":"Durum Wheat","url":"https://www.academia.edu/Documents/in/Durum_Wheat?f_ri=55175"},{"id":991443,"name":"Triticum","url":"https://www.academia.edu/Documents/in/Triticum?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13766520 coauthored" data-work_id="13766520" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13766520/Anti_Inflammatory_and_Antioxidant_Properties_of_a_New_Arylidene_Thiazolidinedione_in_Macrophages">Anti-Inflammatory and Antioxidant Properties of a New Arylidene-Thiazolidinedione in Macrophages</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The anti-in ammatory and antioxidant activities of the aerial part of Helichrysum italicum extracts have been established in various in-vivo and in-vitro experimental models. The results obtained on the acute oedemas induced by... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13766520" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The anti-in ammatory and antioxidant activities of the aerial part of Helichrysum italicum extracts have been established in various in-vivo and in-vitro experimental models. The results obtained on the acute oedemas induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) and ethyl phenylpropiolate in the mouse ear, by serotonin and phospholipase A 2 (PLA 2 ) in the mouse paw, on chronic in ammation induced by repeated application of TPA in the mouse ear and on the delayed-type hypersensitivity induced by sheep red blood cells suggest that said anti-in ammatory activity is due to the effects of compounds expressed via a corticoid-like mechanism. In addition, the antioxidant activity of the extracts seems to be implicated in this anti-in ammatory activity, as the former inhibits enzymatic and non-enzymatic lipid peroxidation and has free-radical scavenger properties. We conclude that the antiin ammatory activity of Helichrysum italicum can be explained by multiple effects, including in ammatory enzyme inhibition, free-radical scavenging activity and corticoid-like effects.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13766520" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bd7730e726c9fbecaa6f37b21fbbf202" rel="nofollow" data-download="{"attachment_id":44976799,"asset_id":13766520,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44976799/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32832947" href="https://unisul.academia.edu/FabriciaPetronilho">Fabricia Petronilho</a><script data-card-contents-for-user="32832947" type="text/json">{"id":32832947,"first_name":"Fabricia","last_name":"Petronilho","domain_name":"unisul","page_name":"FabriciaPetronilho","display_name":"Fabricia Petronilho","profile_url":"https://unisul.academia.edu/FabriciaPetronilho?f_ri=55175","photo":"https://0.academia-photos.com/32832947/9778295/10894210/s65_fabricia.petronilho.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13766520">+1</span><div class="hidden js-additional-users-13766520"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/DriellyFlorentino">Drielly Florentino</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13766520'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13766520').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_13766520 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13766520"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13766520, container: ".js-paper-rank-work_13766520", }); });</script></li><li class="js-percentile-work_13766520 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13766520; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_13766520"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_13766520 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="13766520"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13766520; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13766520]").text(description); $(".js-view-count-work_13766520").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_13766520").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="13766520"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">80</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="656" rel="nofollow" href="https://www.academia.edu/Documents/in/Pharmacy">Pharmacy</a>, <script data-card-contents-for-ri="656" type="text/json">{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1290" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2215" rel="nofollow" href="https://www.academia.edu/Documents/in/Water">Water</a>, <script data-card-contents-for-ri="2215" type="text/json">{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4083" rel="nofollow" href="https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine">Complementary and Alternative Medicine</a><script data-card-contents-for-ri="4083" type="text/json">{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13766520]'), work: {"id":13766520,"title":"Anti-Inflammatory and Antioxidant Properties of a New Arylidene-Thiazolidinedione in Macrophages","created_at":"2015-07-07T14:18:59.301-07:00","url":"https://www.academia.edu/13766520/Anti_Inflammatory_and_Antioxidant_Properties_of_a_New_Arylidene_Thiazolidinedione_in_Macrophages?f_ri=55175","dom_id":"work_13766520","summary":"The anti-in ammatory and antioxidant activities of the aerial part of Helichrysum italicum extracts have been established in various in-vivo and in-vitro experimental models. The results obtained on the acute oedemas induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) and ethyl phenylpropiolate in the mouse ear, by serotonin and phospholipase A 2 (PLA 2 ) in the mouse paw, on chronic in ammation induced by repeated application of TPA in the mouse ear and on the delayed-type hypersensitivity induced by sheep red blood cells suggest that said anti-in ammatory activity is due to the effects of compounds expressed via a corticoid-like mechanism. In addition, the antioxidant activity of the extracts seems to be implicated in this anti-in ammatory activity, as the former inhibits enzymatic and non-enzymatic lipid peroxidation and has free-radical scavenger properties. We conclude that the antiin ammatory activity of Helichrysum italicum can be explained by multiple effects, including in ammatory enzyme inhibition, free-radical scavenging activity and corticoid-like effects.","downloadable_attachments":[{"id":44976799,"asset_id":13766520,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32832947,"first_name":"Fabricia","last_name":"Petronilho","domain_name":"unisul","page_name":"FabriciaPetronilho","display_name":"Fabricia Petronilho","profile_url":"https://unisul.academia.edu/FabriciaPetronilho?f_ri=55175","photo":"https://0.academia-photos.com/32832947/9778295/10894210/s65_fabricia.petronilho.jpg"},{"id":32879345,"first_name":"Drielly","last_name":"Florentino","domain_name":"independent","page_name":"DriellyFlorentino","display_name":"Drielly Florentino","profile_url":"https://independent.academia.edu/DriellyFlorentino?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy?f_ri=55175","nofollow":true},{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=55175","nofollow":true},{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=55175","nofollow":true},{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175","nofollow":true},{"id":4581,"name":"Diabetes","url":"https://www.academia.edu/Documents/in/Diabetes?f_ri=55175"},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175"},{"id":8015,"name":"Atherosclerosis","url":"https://www.academia.edu/Documents/in/Atherosclerosis?f_ri=55175"},{"id":8991,"name":"Wound Healing","url":"https://www.academia.edu/Documents/in/Wound_Healing?f_ri=55175"},{"id":9111,"name":"Cytokines","url":"https://www.academia.edu/Documents/in/Cytokines?f_ri=55175"},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=55175"},{"id":14032,"name":"Ethnopharmacology","url":"https://www.academia.edu/Documents/in/Ethnopharmacology?f_ri=55175"},{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=55175"},{"id":17491,"name":"Macrophages","url":"https://www.academia.edu/Documents/in/Macrophages?f_ri=55175"},{"id":37782,"name":"Cell Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture?f_ri=55175"},{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=55175"},{"id":52027,"name":"Phytotherapy","url":"https://www.academia.edu/Documents/in/Phytotherapy?f_ri=55175"},{"id":52055,"name":"Lipids","url":"https://www.academia.edu/Documents/in/Lipids?f_ri=55175"},{"id":55163,"name":"Enzymes","url":"https://www.academia.edu/Documents/in/Enzymes?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":71300,"name":"Blood Glucose","url":"https://www.academia.edu/Documents/in/Blood_Glucose?f_ri=55175"},{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=55175"},{"id":71510,"name":"Endothelial Cells","url":"https://www.academia.edu/Documents/in/Endothelial_Cells?f_ri=55175"},{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids?f_ri=55175"},{"id":82978,"name":"Reactive Oxygen Species","url":"https://www.academia.edu/Documents/in/Reactive_Oxygen_Species?f_ri=55175"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=55175"},{"id":84945,"name":"Pharmacology and Clinical pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacology_and_Clinical_pharmacy?f_ri=55175"},{"id":88062,"name":"Vasculitis","url":"https://www.academia.edu/Documents/in/Vasculitis?f_ri=55175"},{"id":90002,"name":"Renin Angiotensin Aldosterone System","url":"https://www.academia.edu/Documents/in/Renin_Angiotensin_Aldosterone_System?f_ri=55175"},{"id":121705,"name":"Ethanol","url":"https://www.academia.edu/Documents/in/Ethanol?f_ri=55175"},{"id":122402,"name":"Nitrates","url":"https://www.academia.edu/Documents/in/Nitrates?f_ri=55175"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=55175"},{"id":141395,"name":"Glutathione Peroxidase","url":"https://www.academia.edu/Documents/in/Glutathione_Peroxidase?f_ri=55175"},{"id":146242,"name":"Urea","url":"https://www.academia.edu/Documents/in/Urea?f_ri=55175"},{"id":151086,"name":"Peptides","url":"https://www.academia.edu/Documents/in/Peptides?f_ri=55175"},{"id":160814,"name":"Fruit","url":"https://www.academia.edu/Documents/in/Fruit?f_ri=55175"},{"id":162553,"name":"Skin","url":"https://www.academia.edu/Documents/in/Skin?f_ri=55175"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=55175"},{"id":202399,"name":"Plant Roots","url":"https://www.academia.edu/Documents/in/Plant_Roots?f_ri=55175"},{"id":204350,"name":"Anti-inflammatory agents","url":"https://www.academia.edu/Documents/in/Anti-inflammatory_agents?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":233372,"name":"Lipid peroxidation","url":"https://www.academia.edu/Documents/in/Lipid_peroxidation?f_ri=55175"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=55175"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=55175"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide?f_ri=55175"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=55175"},{"id":297791,"name":"Malvaceae","url":"https://www.academia.edu/Documents/in/Malvaceae?f_ri=55175"},{"id":308667,"name":"Pharmaceutical Biology","url":"https://www.academia.edu/Documents/in/Pharmaceutical_Biology?f_ri=55175"},{"id":309088,"name":"Uric Acid","url":"https://www.academia.edu/Documents/in/Uric_Acid?f_ri=55175"},{"id":335983,"name":"Lipopolysaccharides","url":"https://www.academia.edu/Documents/in/Lipopolysaccharides?f_ri=55175"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":382481,"name":"Edema","url":"https://www.academia.edu/Documents/in/Edema?f_ri=55175"},{"id":401214,"name":"Endothelial cell","url":"https://www.academia.edu/Documents/in/Endothelial_cell?f_ri=55175"},{"id":427871,"name":"Oxidative Damage","url":"https://www.academia.edu/Documents/in/Oxidative_Damage?f_ri=55175"},{"id":449523,"name":"Foot","url":"https://www.academia.edu/Documents/in/Foot?f_ri=55175"},{"id":453923,"name":"Oral Hypoglycemic Agents","url":"https://www.academia.edu/Documents/in/Oral_Hypoglycemic_Agents?f_ri=55175"},{"id":474029,"name":"Tumor necrosis factor-alpha","url":"https://www.academia.edu/Documents/in/Tumor_necrosis_factor-alpha?f_ri=55175"},{"id":564879,"name":"Wistar Rats","url":"https://www.academia.edu/Documents/in/Wistar_Rats?f_ri=55175"},{"id":568887,"name":"Carrageenan","url":"https://www.academia.edu/Documents/in/Carrageenan?f_ri=55175"},{"id":581656,"name":"Rhizome","url":"https://www.academia.edu/Documents/in/Rhizome?f_ri=55175"},{"id":598971,"name":"Ginger","url":"https://www.academia.edu/Documents/in/Ginger?f_ri=55175"},{"id":731133,"name":"Curcuma","url":"https://www.academia.edu/Documents/in/Curcuma?f_ri=55175"},{"id":801413,"name":"Benzimidazoles","url":"https://www.academia.edu/Documents/in/Benzimidazoles?f_ri=55175"},{"id":859027,"name":"Adhesion molecules","url":"https://www.academia.edu/Documents/in/Adhesion_molecules?f_ri=55175"},{"id":886644,"name":"Oxidant","url":"https://www.academia.edu/Documents/in/Oxidant?f_ri=55175"},{"id":915951,"name":"Type 2 Diabetes Mellitus","url":"https://www.academia.edu/Documents/in/Type_2_Diabetes_Mellitus?f_ri=55175"},{"id":954841,"name":"Angiotensin II","url":"https://www.academia.edu/Documents/in/Angiotensin_II?f_ri=55175"},{"id":965028,"name":"Cell free System","url":"https://www.academia.edu/Documents/in/Cell_free_System?f_ri=55175"},{"id":1272099,"name":"Leukocytes","url":"https://www.academia.edu/Documents/in/Leukocytes?f_ri=55175"},{"id":1281380,"name":"Platelet Count","url":"https://www.academia.edu/Documents/in/Platelet_Count?f_ri=55175"},{"id":1311261,"name":"Hemoglobins","url":"https://www.academia.edu/Documents/in/Hemoglobins?f_ri=55175"},{"id":1352490,"name":"Oxidants","url":"https://www.academia.edu/Documents/in/Oxidants?f_ri=55175"},{"id":1438730,"name":"Creatinine","url":"https://www.academia.edu/Documents/in/Creatinine?f_ri=55175"},{"id":1654024,"name":"Nitrites","url":"https://www.academia.edu/Documents/in/Nitrites?f_ri=55175"},{"id":1745595,"name":"Solvents","url":"https://www.academia.edu/Documents/in/Solvents?f_ri=55175"},{"id":1819399,"name":"Case Control Studies","url":"https://www.academia.edu/Documents/in/Case_Control_Studies?f_ri=55175"},{"id":1866509,"name":"Indomethacin","url":"https://www.academia.edu/Documents/in/Indomethacin?f_ri=55175"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves?f_ri=55175"},{"id":2263783,"name":"Globulins","url":"https://www.academia.edu/Documents/in/Globulins?f_ri=55175"},{"id":2349258,"name":"Serum albumin","url":"https://www.academia.edu/Documents/in/Serum_albumin?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24088385" data-work_id="24088385" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24088385/Gingival_crevicular_fluid_levels_of_MMP_8_MMP_9_TIMP_2_and_MPO_decrease_after_periodontal_therapy">Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy Marcaccini AM, Meschiari CA, Zuardi LR, de Sousa TS, Taba M, Teofilo JM, Jacob-Ferreira ALB, Tanus-Santos JE, Novaes AB, Gerlach RF.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24088385" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy Marcaccini AM, Meschiari CA, Zuardi LR, de Sousa TS, Taba M, Teofilo JM, Jacob-Ferreira ALB, Tanus-Santos JE, Novaes AB, Gerlach RF. Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24088385" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8a8120835f96959b31fa2f0846602557" rel="nofollow" data-download="{"attachment_id":44455370,"asset_id":24088385,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44455370/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46479752" href="https://usp-br1.academia.edu/AndreaMarcaccini">Andrea Marcaccini</a><script data-card-contents-for-user="46479752" type="text/json">{"id":46479752,"first_name":"Andrea","last_name":"Marcaccini","domain_name":"usp-br1","page_name":"AndreaMarcaccini","display_name":"Andrea Marcaccini","profile_url":"https://usp-br1.academia.edu/AndreaMarcaccini?f_ri=55175","photo":"https://0.academia-photos.com/46479752/17955893/30123538/s65_andrea.marcaccini.jpg"}</script></span></span></li><li class="js-paper-rank-work_24088385 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24088385"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24088385, container: ".js-paper-rank-work_24088385", }); });</script></li><li class="js-percentile-work_24088385 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24088385; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_24088385"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_24088385 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="24088385"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24088385; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24088385]").text(description); $(".js-view-count-work_24088385").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24088385").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24088385"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="596" rel="nofollow" href="https://www.academia.edu/Documents/in/Dentistry">Dentistry</a>, <script data-card-contents-for-ri="596" type="text/json">{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3243" rel="nofollow" href="https://www.academia.edu/Documents/in/Nonparametric_Statistics">Nonparametric Statistics</a>, <script data-card-contents-for-ri="3243" type="text/json">{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12426" rel="nofollow" href="https://www.academia.edu/Documents/in/Treatment_Outcome">Treatment Outcome</a>, <script data-card-contents-for-ri="12426" type="text/json">{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24088385]'), work: {"id":24088385,"title":"Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy","created_at":"2016-04-05T18:39:01.277-07:00","url":"https://www.academia.edu/24088385/Gingival_crevicular_fluid_levels_of_MMP_8_MMP_9_TIMP_2_and_MPO_decrease_after_periodontal_therapy?f_ri=55175","dom_id":"work_24088385","summary":"Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy Marcaccini AM, Meschiari CA, Zuardi LR, de Sousa TS, Taba M, Teofilo JM, Jacob-Ferreira ALB, Tanus-Santos JE, Novaes AB, Gerlach RF. Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy.","downloadable_attachments":[{"id":44455370,"asset_id":24088385,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":46479752,"first_name":"Andrea","last_name":"Marcaccini","domain_name":"usp-br1","page_name":"AndreaMarcaccini","display_name":"Andrea Marcaccini","profile_url":"https://usp-br1.academia.edu/AndreaMarcaccini?f_ri=55175","photo":"https://0.academia-photos.com/46479752/17955893/30123538/s65_andrea.marcaccini.jpg"}],"research_interests":[{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=55175","nofollow":true},{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics?f_ri=55175","nofollow":true},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":99708,"name":"Clinical","url":"https://www.academia.edu/Documents/in/Clinical?f_ri=55175"},{"id":375098,"name":"Gingiva","url":"https://www.academia.edu/Documents/in/Gingiva?f_ri=55175"},{"id":724446,"name":"Gingival Crevicular Fluid","url":"https://www.academia.edu/Documents/in/Gingival_Crevicular_Fluid?f_ri=55175"},{"id":855633,"name":"Chronic Periodontitis","url":"https://www.academia.edu/Documents/in/Chronic_Periodontitis?f_ri=55175"},{"id":1000427,"name":"Reference Values","url":"https://www.academia.edu/Documents/in/Reference_Values?f_ri=55175"},{"id":1819399,"name":"Case Control Studies","url":"https://www.academia.edu/Documents/in/Case_Control_Studies?f_ri=55175"},{"id":1920779,"name":"Matrix Metalloproteinase","url":"https://www.academia.edu/Documents/in/Matrix_Metalloproteinase?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20718973" data-work_id="20718973" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20718973/Remittent_non_destructive_polysynovitis_in_P_ANCA_positive_vasculitis_patients_with_anti_CCP_antibodies">Remittent non-destructive polysynovitis in P-ANCA-positive vasculitis patients with anti-CCP antibodies</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Patients with antineutrophil cytoplasm antibodies (ANCA)-associated vasculitis (AASV) commonly suffer from arthralgias and, sometimes, polyarthritis during disease flares. Although rheumatoid factor (RF) can be detected in up to 37-50% of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_20718973" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Patients with antineutrophil cytoplasm antibodies (ANCA)-associated vasculitis (AASV) commonly suffer from arthralgias and, sometimes, polyarthritis during disease flares. Although rheumatoid factor (RF) can be detected in up to 37-50% of AASV patients, anti-cyclic citrullinated peptide (anti-CCP) antibodies are rare. Herein, we describe the clinical features of five P-ANCA-positive vasculitis patients, who had persistent and/or high anti-CCP levels and, more importantly, suffered from remittent non-destructive arthralgias and polysynovitis, independently of the vasculitis course and without evidence of RA. Two were initially thought to have RA rather than microscopic polyangiitis and, at AASV diagnosis, all had kidney involvement and three had alveolar hemorrhages. With a median follow-up of 30 months, one suffered vasculitis relapses, preceded by polysynovitis, and others had remittent arthralgias and polysynovitis, while their vasculitides remained in remission. None of these patients had radiological destructive arthritis. We discuss the challenge of diagnosing these patients positive for anti-CCP and ANCA, and with dominant articular manifestations. AASV patients with anti-CCP antibodies may experience relapsing polysynovitis and non-erosive polyarthritis prior to vasculitis flares, but also independently of the vasculitis course, which is uncommon in AASV, and might represent a small subgroup of AASV patients.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20718973" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="65d2915b97c57fc754f47458c2bf7162" rel="nofollow" data-download="{"attachment_id":41930424,"asset_id":20718973,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41930424/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41928299" href="https://independent.academia.edu/SamirRouabhia">Samir Rouabhia</a><script data-card-contents-for-user="41928299" type="text/json">{"id":41928299,"first_name":"Samir","last_name":"Rouabhia","domain_name":"independent","page_name":"SamirRouabhia","display_name":"Samir Rouabhia","profile_url":"https://independent.academia.edu/SamirRouabhia?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_20718973 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="20718973"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 20718973, container: ".js-paper-rank-work_20718973", }); });</script></li><li class="js-percentile-work_20718973 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20718973; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_20718973"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_20718973 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="20718973"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20718973; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20718973]").text(description); $(".js-view-count-work_20718973").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_20718973").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="20718973"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="12733" rel="nofollow" href="https://www.academia.edu/Documents/in/Rheumatoid_Arthritis">Rheumatoid Arthritis</a>, <script data-card-contents-for-ri="12733" type="text/json">{"id":12733,"name":"Rheumatoid Arthritis","url":"https://www.academia.edu/Documents/in/Rheumatoid_Arthritis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="88062" rel="nofollow" href="https://www.academia.edu/Documents/in/Vasculitis">Vasculitis</a>, <script data-card-contents-for-ri="88062" type="text/json">{"id":88062,"name":"Vasculitis","url":"https://www.academia.edu/Documents/in/Vasculitis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="158399" rel="nofollow" href="https://www.academia.edu/Documents/in/Cyclic_peptides">Cyclic peptides</a><script data-card-contents-for-ri="158399" type="text/json">{"id":158399,"name":"Cyclic peptides","url":"https://www.academia.edu/Documents/in/Cyclic_peptides?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20718973]'), work: {"id":20718973,"title":"Remittent non-destructive polysynovitis in P-ANCA-positive vasculitis patients with anti-CCP antibodies","created_at":"2016-01-24T12:46:04.719-08:00","url":"https://www.academia.edu/20718973/Remittent_non_destructive_polysynovitis_in_P_ANCA_positive_vasculitis_patients_with_anti_CCP_antibodies?f_ri=55175","dom_id":"work_20718973","summary":"Patients with antineutrophil cytoplasm antibodies (ANCA)-associated vasculitis (AASV) commonly suffer from arthralgias and, sometimes, polyarthritis during disease flares. Although rheumatoid factor (RF) can be detected in up to 37-50% of AASV patients, anti-cyclic citrullinated peptide (anti-CCP) antibodies are rare. Herein, we describe the clinical features of five P-ANCA-positive vasculitis patients, who had persistent and/or high anti-CCP levels and, more importantly, suffered from remittent non-destructive arthralgias and polysynovitis, independently of the vasculitis course and without evidence of RA. Two were initially thought to have RA rather than microscopic polyangiitis and, at AASV diagnosis, all had kidney involvement and three had alveolar hemorrhages. With a median follow-up of 30 months, one suffered vasculitis relapses, preceded by polysynovitis, and others had remittent arthralgias and polysynovitis, while their vasculitides remained in remission. None of these patients had radiological destructive arthritis. We discuss the challenge of diagnosing these patients positive for anti-CCP and ANCA, and with dominant articular manifestations. AASV patients with anti-CCP antibodies may experience relapsing polysynovitis and non-erosive polyarthritis prior to vasculitis flares, but also independently of the vasculitis course, which is uncommon in AASV, and might represent a small subgroup of AASV patients.","downloadable_attachments":[{"id":41930424,"asset_id":20718973,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41928299,"first_name":"Samir","last_name":"Rouabhia","domain_name":"independent","page_name":"SamirRouabhia","display_name":"Samir Rouabhia","profile_url":"https://independent.academia.edu/SamirRouabhia?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":12733,"name":"Rheumatoid Arthritis","url":"https://www.academia.edu/Documents/in/Rheumatoid_Arthritis?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":88062,"name":"Vasculitis","url":"https://www.academia.edu/Documents/in/Vasculitis?f_ri=55175","nofollow":true},{"id":158399,"name":"Cyclic peptides","url":"https://www.academia.edu/Documents/in/Cyclic_peptides?f_ri=55175","nofollow":true},{"id":234187,"name":"Recurrence","url":"https://www.academia.edu/Documents/in/Recurrence?f_ri=55175"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=55175"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=55175"},{"id":568482,"name":"Biological markers","url":"https://www.academia.edu/Documents/in/Biological_markers?f_ri=55175"},{"id":964185,"name":"Synovitis","url":"https://www.academia.edu/Documents/in/Synovitis?f_ri=55175"},{"id":1235144,"name":"ANCA Associated Vasculitis","url":"https://www.academia.edu/Documents/in/ANCA_Associated_Vasculitis?f_ri=55175"},{"id":1401310,"name":"Myeloperoxidase","url":"https://www.academia.edu/Documents/in/Myeloperoxidase?f_ri=55175"},{"id":1413449,"name":"Microscopic Polyangiitis Market","url":"https://www.academia.edu/Documents/in/Microscopic_Polyangiitis_Market?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9916985" data-work_id="9916985" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9916985/Peroxidase_mediated_formation_of_resveratrol_oxidation_products_during_the_hypersensitive_like_reaction_of_grapevine_cells_to_an_elicitor_from_Trichoderma_viride">Peroxidase-mediated formation of resveratrol oxidation products during the hypersensitive-like reaction of grapevine cells to an elicitor from Trichoderma viride</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9916985" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4ae5daea6a3b5c78783e0ab5068be019" rel="nofollow" data-download="{"attachment_id":47605986,"asset_id":9916985,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47605986/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="24082866" href="https://independent.academia.edu/josezapata10">jose zapata</a><script data-card-contents-for-user="24082866" type="text/json">{"id":24082866,"first_name":"jose","last_name":"zapata","domain_name":"independent","page_name":"josezapata10","display_name":"jose zapata","profile_url":"https://independent.academia.edu/josezapata10?f_ri=55175","photo":"https://0.academia-photos.com/24082866/6499993/7350385/s65_jose.zapata.jpg"}</script></span></span></li><li class="js-paper-rank-work_9916985 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="9916985"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 9916985, container: ".js-paper-rank-work_9916985", }); });</script></li><li class="js-percentile-work_9916985 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 9916985; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_9916985"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_9916985 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="9916985"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 9916985; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=9916985]").text(description); $(".js-view-count-work_9916985").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_9916985").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="9916985"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">3</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=9916985]'), work: {"id":9916985,"title":"Peroxidase-mediated formation of resveratrol oxidation products during the hypersensitive-like reaction of grapevine cells to an elicitor from Trichoderma viride","created_at":"2014-12-27T08:20:38.668-08:00","url":"https://www.academia.edu/9916985/Peroxidase_mediated_formation_of_resveratrol_oxidation_products_during_the_hypersensitive_like_reaction_of_grapevine_cells_to_an_elicitor_from_Trichoderma_viride?f_ri=55175","dom_id":"work_9916985","summary":null,"downloadable_attachments":[{"id":47605986,"asset_id":9916985,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":24082866,"first_name":"jose","last_name":"zapata","domain_name":"independent","page_name":"josezapata10","display_name":"jose zapata","profile_url":"https://independent.academia.edu/josezapata10?f_ri=55175","photo":"https://0.academia-photos.com/24082866/6499993/7350385/s65_jose.zapata.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=55175","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24257369" data-work_id="24257369" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24257369/Toxoplasma_gondii_infection_of_the_central_nervous_system">Toxoplasma gondii infection of the central nervous system</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The l)eroxidase-antiperoxidase immunolfistocllemical tecimique was employed to stain fi~rmalin fixed, paraffin embedded tissue sections from three cases of enceplmlitis caused by Toxoplasma gondii. We studied two cases of congenital... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24257369" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The l)eroxidase-antiperoxidase immunolfistocllemical tecimique was employed to stain fi~rmalin fixed, paraffin embedded tissue sections from three cases of enceplmlitis caused by Toxoplasma gondii. We studied two cases of congenital infection and one case of acquired toxoplasmosis occurring in an immunoconapromised host. The peroxidase-antiperoxidase method was exquisitely sensitive and highly specific and stained both the encysted and tachyzoite forms of the organism, as well as allowing for easy identification of infected cells. In two cases of necrotizing encel~halitis--4me congenital, the other acqtfired--widespread dis-</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24257369" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="84c8f2379d9ce968be19237a76872705" rel="nofollow" data-download="{"attachment_id":44594676,"asset_id":24257369,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44594676/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46752999" href="https://independent.academia.edu/JRemington1">J. Remington</a><script data-card-contents-for-user="46752999" type="text/json">{"id":46752999,"first_name":"J.","last_name":"Remington","domain_name":"independent","page_name":"JRemington1","display_name":"J. Remington","profile_url":"https://independent.academia.edu/JRemington1?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24257369 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24257369"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24257369, container: ".js-paper-rank-work_24257369", }); });</script></li><li class="js-percentile-work_24257369 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24257369; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_24257369"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_24257369 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="24257369"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24257369; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24257369]").text(description); $(".js-view-count-work_24257369").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24257369").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24257369"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="13346" rel="nofollow" href="https://www.academia.edu/Documents/in/Toxoplasma">Toxoplasma</a>, <script data-card-contents-for-ri="13346" type="text/json">{"id":13346,"name":"Toxoplasma","url":"https://www.academia.edu/Documents/in/Toxoplasma?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37798" rel="nofollow" href="https://www.academia.edu/Documents/in/Toxoplasmosis">Toxoplasmosis</a>, <script data-card-contents-for-ri="37798" type="text/json">{"id":37798,"name":"Toxoplasmosis","url":"https://www.academia.edu/Documents/in/Toxoplasmosis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37801" rel="nofollow" href="https://www.academia.edu/Documents/in/Toxoplasma_gondii">Toxoplasma gondii</a>, <script data-card-contents-for-ri="37801" type="text/json">{"id":37801,"name":"Toxoplasma gondii","url":"https://www.academia.edu/Documents/in/Toxoplasma_gondii?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24257369]'), work: {"id":24257369,"title":"Toxoplasma gondii infection of the central nervous system","created_at":"2016-04-10T08:17:53.689-07:00","url":"https://www.academia.edu/24257369/Toxoplasma_gondii_infection_of_the_central_nervous_system?f_ri=55175","dom_id":"work_24257369","summary":"The l)eroxidase-antiperoxidase immunolfistocllemical tecimique was employed to stain fi~rmalin fixed, paraffin embedded tissue sections from three cases of enceplmlitis caused by Toxoplasma gondii. We studied two cases of congenital infection and one case of acquired toxoplasmosis occurring in an immunoconapromised host. The peroxidase-antiperoxidase method was exquisitely sensitive and highly specific and stained both the encysted and tachyzoite forms of the organism, as well as allowing for easy identification of infected cells. In two cases of necrotizing encel~halitis--4me congenital, the other acqtfired--widespread dis-","downloadable_attachments":[{"id":44594676,"asset_id":24257369,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":46752999,"first_name":"J.","last_name":"Remington","domain_name":"independent","page_name":"JRemington1","display_name":"J. Remington","profile_url":"https://independent.academia.edu/JRemington1?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":13346,"name":"Toxoplasma","url":"https://www.academia.edu/Documents/in/Toxoplasma?f_ri=55175","nofollow":true},{"id":37798,"name":"Toxoplasmosis","url":"https://www.academia.edu/Documents/in/Toxoplasmosis?f_ri=55175","nofollow":true},{"id":37801,"name":"Toxoplasma gondii","url":"https://www.academia.edu/Documents/in/Toxoplasma_gondii?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":61474,"name":"Brain","url":"https://www.academia.edu/Documents/in/Brain?f_ri=55175"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=55175"},{"id":147195,"name":"Central Nervous System","url":"https://www.academia.edu/Documents/in/Central_Nervous_System?f_ri=55175"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=55175"},{"id":253560,"name":"Newborn Infant","url":"https://www.academia.edu/Documents/in/Newborn_Infant?f_ri=55175"},{"id":372398,"name":"Paraffin","url":"https://www.academia.edu/Documents/in/Paraffin?f_ri=55175"},{"id":674257,"name":"Human Pathology","url":"https://www.academia.edu/Documents/in/Human_Pathology?f_ri=55175"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits?f_ri=55175"},{"id":819116,"name":"Formaldehyde","url":"https://www.academia.edu/Documents/in/Formaldehyde?f_ri=55175"},{"id":1036479,"name":"Fixatives","url":"https://www.academia.edu/Documents/in/Fixatives?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6511863" data-work_id="6511863" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6511863/Antioxidant_activity_of_methanolic_extract_of_Bombax_ceiba">Antioxidant activity of methanolic extract of Bombax ceiba</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The antioxidant activity of a methanolic extract of Bombax ceiba was evaluated using several antioxidant assays, in terms of its: (i) ability to scavenge DPPH (1,1-diphenyl-2-picryl-hydrazyl) and hydroxyl free radicals; (ii) action... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6511863" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The antioxidant activity of a methanolic extract of Bombax ceiba was evaluated using several antioxidant assays, in terms of its: (i) ability to scavenge DPPH (1,1-diphenyl-2-picryl-hydrazyl) and hydroxyl free radicals; (ii) action against lipid peroxidation (in rat liver microsomes and soy bean phosphatidylcholine liposomes), induced by ascorbyl radicals and peroxynitrite; and (iii) effect on myeloperoxidase activity. The cytotoxicity was monitored through the mitochondrial activity in the Vero cell line. The extract showed antioxidant activity in all assays, the EC 50 (µg/ml) for DPPH was 87 and for lipid peroxidation of microsomes and soy bean liposomes induced by ascorbyl radicals were 141 and 105, respectively, and by peroxynitrite were 115 and 77, respectively. The K 0.5 value for myeloperoxidase activity inhibition by the extract was 264 µg/ml. The extract showed very low toxicity toward Vero cells.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6511863" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="947e954462469f99838d6190fc5c735a" rel="nofollow" data-download="{"attachment_id":40258167,"asset_id":6511863,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40258167/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10394876" href="https://wwwnrc.academia.edu/MonaAzzam">Mona Azzam</a><script data-card-contents-for-user="10394876" type="text/json">{"id":10394876,"first_name":"Mona","last_name":"Azzam","domain_name":"wwwnrc","page_name":"MonaAzzam","display_name":"Mona Azzam","profile_url":"https://wwwnrc.academia.edu/MonaAzzam?f_ri=55175","photo":"https://0.academia-photos.com/10394876/4715360/5439441/s65_mona.azzam.jpg_oh_7c13d1383b57d1f26e810877cb0edcf6_oe_5494fb88___gda___1417835331_c2e3a2c0ed6e3eb6830eedca2716a9f7"}</script></span></span></li><li class="js-paper-rank-work_6511863 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6511863"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6511863, container: ".js-paper-rank-work_6511863", }); });</script></li><li class="js-percentile-work_6511863 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 6511863; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_6511863"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_6511863 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="6511863"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 6511863; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=6511863]").text(description); $(".js-view-count-work_6511863").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6511863").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6511863"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">35</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="524" rel="nofollow" href="https://www.academia.edu/Documents/in/Analytical_Chemistry">Analytical Chemistry</a>, <script data-card-contents-for-ri="524" type="text/json">{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2425" rel="nofollow" href="https://www.academia.edu/Documents/in/Free_Radicals">Free Radicals</a>, <script data-card-contents-for-ri="2425" type="text/json">{"id":2425,"name":"Free Radicals","url":"https://www.academia.edu/Documents/in/Free_Radicals?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4083" rel="nofollow" href="https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine">Complementary and Alternative Medicine</a>, <script data-card-contents-for-ri="4083" type="text/json">{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a><script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6511863]'), work: {"id":6511863,"title":"Antioxidant activity of methanolic extract of Bombax ceiba","created_at":"2014-03-22T23:57:02.344-07:00","url":"https://www.academia.edu/6511863/Antioxidant_activity_of_methanolic_extract_of_Bombax_ceiba?f_ri=55175","dom_id":"work_6511863","summary":"The antioxidant activity of a methanolic extract of Bombax ceiba was evaluated using several antioxidant assays, in terms of its: (i) ability to scavenge DPPH (1,1-diphenyl-2-picryl-hydrazyl) and hydroxyl free radicals; (ii) action against lipid peroxidation (in rat liver microsomes and soy bean phosphatidylcholine liposomes), induced by ascorbyl radicals and peroxynitrite; and (iii) effect on myeloperoxidase activity. The cytotoxicity was monitored through the mitochondrial activity in the Vero cell line. The extract showed antioxidant activity in all assays, the EC 50 (µg/ml) for DPPH was 87 and for lipid peroxidation of microsomes and soy bean liposomes induced by ascorbyl radicals were 141 and 105, respectively, and by peroxynitrite were 115 and 77, respectively. The K 0.5 value for myeloperoxidase activity inhibition by the extract was 264 µg/ml. The extract showed very low toxicity toward Vero cells.","downloadable_attachments":[{"id":40258167,"asset_id":6511863,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":10394876,"first_name":"Mona","last_name":"Azzam","domain_name":"wwwnrc","page_name":"MonaAzzam","display_name":"Mona Azzam","profile_url":"https://wwwnrc.academia.edu/MonaAzzam?f_ri=55175","photo":"https://0.academia-photos.com/10394876/4715360/5439441/s65_mona.azzam.jpg_oh_7c13d1383b57d1f26e810877cb0edcf6_oe_5494fb88___gda___1417835331_c2e3a2c0ed6e3eb6830eedca2716a9f7"}],"research_interests":[{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=55175","nofollow":true},{"id":2425,"name":"Free Radicals","url":"https://www.academia.edu/Documents/in/Free_Radicals?f_ri=55175","nofollow":true},{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175"},{"id":12108,"name":"China","url":"https://www.academia.edu/Documents/in/China?f_ri=55175"},{"id":22052,"name":"Flavonoids","url":"https://www.academia.edu/Documents/in/Flavonoids?f_ri=55175"},{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=55175"},{"id":52027,"name":"Phytotherapy","url":"https://www.academia.edu/Documents/in/Phytotherapy?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":57570,"name":"Cercopithecus aethiops","url":"https://www.academia.edu/Documents/in/Cercopithecus_aethiops?f_ri=55175"},{"id":90451,"name":"African","url":"https://www.academia.edu/Documents/in/African?f_ri=55175"},{"id":112334,"name":"Methanol","url":"https://www.academia.edu/Documents/in/Methanol?f_ri=55175"},{"id":147066,"name":"Liposomes","url":"https://www.academia.edu/Documents/in/Liposomes?f_ri=55175"},{"id":158597,"name":"Iron","url":"https://www.academia.edu/Documents/in/Iron?f_ri=55175"},{"id":180087,"name":"Scavenging","url":"https://www.academia.edu/Documents/in/Scavenging?f_ri=55175"},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=55175"},{"id":233372,"name":"Lipid peroxidation","url":"https://www.academia.edu/Documents/in/Lipid_peroxidation?f_ri=55175"},{"id":290137,"name":"Chine","url":"https://www.academia.edu/Documents/in/Chine?f_ri=55175"},{"id":290433,"name":"Antioxidant Activity","url":"https://www.academia.edu/Documents/in/Antioxidant_Activity?f_ri=55175"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":523688,"name":"Fitoterapia","url":"https://www.academia.edu/Documents/in/Fitoterapia?f_ri=55175"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=55175"},{"id":611814,"name":"Correlation coefficient","url":"https://www.academia.edu/Documents/in/Correlation_coefficient?f_ri=55175"},{"id":739714,"name":"Hypericum","url":"https://www.academia.edu/Documents/in/Hypericum?f_ri=55175"},{"id":789968,"name":"Extract","url":"https://www.academia.edu/Documents/in/Extract?f_ri=55175"},{"id":832148,"name":"Polyphenol","url":"https://www.academia.edu/Documents/in/Polyphenol?f_ri=55175"},{"id":834321,"name":"Phytopharmacy","url":"https://www.academia.edu/Documents/in/Phytopharmacy?f_ri=55175"},{"id":922739,"name":"Pharmacy and Pharmaceutical Sciences","url":"https://www.academia.edu/Documents/in/Pharmacy_and_Pharmaceutical_Sciences?f_ri=55175"},{"id":1035964,"name":"Superoxide Anion","url":"https://www.academia.edu/Documents/in/Superoxide_Anion?f_ri=55175"},{"id":1126694,"name":"Total Phenolics","url":"https://www.academia.edu/Documents/in/Total_Phenolics?f_ri=55175"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=55175"},{"id":1292080,"name":"Vero cells","url":"https://www.academia.edu/Documents/in/Vero_cells?f_ri=55175"},{"id":1379131,"name":"Total Phenolic Content","url":"https://www.academia.edu/Documents/in/Total_Phenolic_Content?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_48021285" data-work_id="48021285" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48021285/Decolourization_of_synthetic_dyes_by_a_newly_isolated_strain_of_Serratia_marcescens">Decolourization of synthetic dyes by a newly isolated strain of Serratia marcescens</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A novel dye-decolourizing strain of the bacterium Serratia marcescens efficiently decolourized two chemically different dyes Ranocid Fast Blue (RFB) and Procion Brilliant Blue-H-GR (PBB-HGR) belonging respectively to the azo and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48021285" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A novel dye-decolourizing strain of the bacterium Serratia marcescens efficiently decolourized two chemically different dyes Ranocid Fast Blue (RFB) and Procion Brilliant Blue-H-GR (PBB-HGR) belonging respectively to the azo and anthraquinone groups. Extracellular laccase and manganese peroxidase (MnP) activity were detected during dye decolourization. The involvement of MnP activity was found in the decolourization of both dyes. More than 90% decolourization of PBB-HGR and RFB was obtained on days 8 and 5, respectively at 26°C under static conditions at pH 7.0. MnP activity was increased by the addition of Mn 2+. At 50 lM Mn 2+ , high MnP (55.3 U/ml) but low laccase activity (8.3 U/ml) was observed. Influence of oxalic acid on MnP activity was also observed.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48021285" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e811ecc0d5aa06de2c0c40940bb34a64" rel="nofollow" data-download="{"attachment_id":66849671,"asset_id":48021285,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/66849671/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="109550937" href="https://independent.academia.edu/DrPradeepVerma">Dr. Pradeep Verma</a><script data-card-contents-for-user="109550937" type="text/json">{"id":109550937,"first_name":"Dr. Pradeep","last_name":"Verma","domain_name":"independent","page_name":"DrPradeepVerma","display_name":"Dr. Pradeep Verma","profile_url":"https://independent.academia.edu/DrPradeepVerma?f_ri=55175","photo":"https://0.academia-photos.com/109550937/31080101/28686922/s65_dr._pradeep.verma.jpg"}</script></span></span></li><li class="js-paper-rank-work_48021285 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48021285"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48021285, container: ".js-paper-rank-work_48021285", }); });</script></li><li class="js-percentile-work_48021285 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 48021285; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_48021285"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_48021285 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="48021285"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 48021285; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=48021285]").text(description); $(".js-view-count-work_48021285").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_48021285").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="48021285"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="923" rel="nofollow" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="203963" rel="nofollow" href="https://www.academia.edu/Documents/in/Laccase">Laccase</a><script data-card-contents-for-ri="203963" type="text/json">{"id":203963,"name":"Laccase","url":"https://www.academia.edu/Documents/in/Laccase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48021285]'), work: {"id":48021285,"title":"Decolourization of synthetic dyes by a newly isolated strain of Serratia marcescens","created_at":"2021-05-03T23:14:19.637-07:00","url":"https://www.academia.edu/48021285/Decolourization_of_synthetic_dyes_by_a_newly_isolated_strain_of_Serratia_marcescens?f_ri=55175","dom_id":"work_48021285","summary":"A novel dye-decolourizing strain of the bacterium Serratia marcescens efficiently decolourized two chemically different dyes Ranocid Fast Blue (RFB) and Procion Brilliant Blue-H-GR (PBB-HGR) belonging respectively to the azo and anthraquinone groups. Extracellular laccase and manganese peroxidase (MnP) activity were detected during dye decolourization. The involvement of MnP activity was found in the decolourization of both dyes. More than 90% decolourization of PBB-HGR and RFB was obtained on days 8 and 5, respectively at 26°C under static conditions at pH 7.0. MnP activity was increased by the addition of Mn 2+. At 50 lM Mn 2+ , high MnP (55.3 U/ml) but low laccase activity (8.3 U/ml) was observed. Influence of oxalic acid on MnP activity was also observed.","downloadable_attachments":[{"id":66849671,"asset_id":48021285,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":109550937,"first_name":"Dr. Pradeep","last_name":"Verma","domain_name":"independent","page_name":"DrPradeepVerma","display_name":"Dr. Pradeep Verma","profile_url":"https://independent.academia.edu/DrPradeepVerma?f_ri=55175","photo":"https://0.academia-photos.com/109550937/31080101/28686922/s65_dr._pradeep.verma.jpg"}],"research_interests":[{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":203963,"name":"Laccase","url":"https://www.academia.edu/Documents/in/Laccase?f_ri=55175","nofollow":true},{"id":413115,"name":"Peroxidases","url":"https://www.academia.edu/Documents/in/Peroxidases?f_ri=55175"},{"id":483712,"name":"Serratia marcescens","url":"https://www.academia.edu/Documents/in/Serratia_marcescens?f_ri=55175"},{"id":1499551,"name":"Oxalic acid","url":"https://www.academia.edu/Documents/in/Oxalic_acid?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_30740703" data-work_id="30740703" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/30740703/Anti_inflammatory_effects_of_methanol_extract_of_Patrinia_scabiosaefolia_in_mice_with_ulcerative_colitis">Anti-inflammatory effects of methanol extract of Patrinia scabiosaefolia in mice with ulcerative colitis</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/30740703" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f992bd59a59df3592e0e4171a3593aec" rel="nofollow" data-download="{"attachment_id":51185474,"asset_id":30740703,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/51185474/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32564308" href="https://unime.academia.edu/MarioVaccaro">Mario Vaccaro</a><script data-card-contents-for-user="32564308" type="text/json">{"id":32564308,"first_name":"Mario","last_name":"Vaccaro","domain_name":"unime","page_name":"MarioVaccaro","display_name":"Mario Vaccaro","profile_url":"https://unime.academia.edu/MarioVaccaro?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_30740703 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="30740703"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 30740703, container: ".js-paper-rank-work_30740703", }); });</script></li><li class="js-percentile-work_30740703 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 30740703; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_30740703"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_30740703 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="30740703"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 30740703; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=30740703]").text(description); $(".js-view-count-work_30740703").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_30740703").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="30740703"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">77</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="140" rel="nofollow" href="https://www.academia.edu/Documents/in/Pharmacology">Pharmacology</a>, <script data-card-contents-for-ri="140" type="text/json">{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="591" rel="nofollow" href="https://www.academia.edu/Documents/in/Nutrition_and_Dietetics">Nutrition and Dietetics</a>, <script data-card-contents-for-ri="591" type="text/json">{"id":591,"name":"Nutrition and Dietetics","url":"https://www.academia.edu/Documents/in/Nutrition_and_Dietetics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1290" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="3867" rel="nofollow" href="https://www.academia.edu/Documents/in/Folk_Medicine">Folk Medicine</a><script data-card-contents-for-ri="3867" type="text/json">{"id":3867,"name":"Folk Medicine","url":"https://www.academia.edu/Documents/in/Folk_Medicine?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=30740703]'), work: {"id":30740703,"title":"Anti-inflammatory effects of methanol extract of Patrinia scabiosaefolia in mice with ulcerative colitis","created_at":"2017-01-04T10:03:48.635-08:00","url":"https://www.academia.edu/30740703/Anti_inflammatory_effects_of_methanol_extract_of_Patrinia_scabiosaefolia_in_mice_with_ulcerative_colitis?f_ri=55175","dom_id":"work_30740703","summary":null,"downloadable_attachments":[{"id":51185474,"asset_id":30740703,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32564308,"first_name":"Mario","last_name":"Vaccaro","domain_name":"unime","page_name":"MarioVaccaro","display_name":"Mario Vaccaro","profile_url":"https://unime.academia.edu/MarioVaccaro?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology?f_ri=55175","nofollow":true},{"id":591,"name":"Nutrition and Dietetics","url":"https://www.academia.edu/Documents/in/Nutrition_and_Dietetics?f_ri=55175","nofollow":true},{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=55175","nofollow":true},{"id":3867,"name":"Folk Medicine","url":"https://www.academia.edu/Documents/in/Folk_Medicine?f_ri=55175","nofollow":true},{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=55175"},{"id":4086,"name":"Traditional Medicine","url":"https://www.academia.edu/Documents/in/Traditional_Medicine?f_ri=55175"},{"id":5072,"name":"Molecular Medicine","url":"https://www.academia.edu/Documents/in/Molecular_Medicine?f_ri=55175"},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175"},{"id":9111,"name":"Cytokines","url":"https://www.academia.edu/Documents/in/Cytokines?f_ri=55175"},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=55175"},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=55175"},{"id":14032,"name":"Ethnopharmacology","url":"https://www.academia.edu/Documents/in/Ethnopharmacology?f_ri=55175"},{"id":14080,"name":"Pharmacognosy","url":"https://www.academia.edu/Documents/in/Pharmacognosy?f_ri=55175"},{"id":17491,"name":"Macrophages","url":"https://www.academia.edu/Documents/in/Macrophages?f_ri=55175"},{"id":23323,"name":"Transcription Factors","url":"https://www.academia.edu/Documents/in/Transcription_Factors?f_ri=55175"},{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression?f_ri=55175"},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=55175"},{"id":44259,"name":"Diarrhea","url":"https://www.academia.edu/Documents/in/Diarrhea?f_ri=55175"},{"id":48183,"name":"Lipopolysaccharide","url":"https://www.academia.edu/Documents/in/Lipopolysaccharide?f_ri=55175"},{"id":49018,"name":"Spleen","url":"https://www.academia.edu/Documents/in/Spleen?f_ri=55175"},{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=55175"},{"id":52027,"name":"Phytotherapy","url":"https://www.academia.edu/Documents/in/Phytotherapy?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=55175"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro?f_ri=55175"},{"id":68228,"name":"Salmonella enteritidis","url":"https://www.academia.edu/Documents/in/Salmonella_enteritidis?f_ri=55175"},{"id":69424,"name":"Protein Kinases","url":"https://www.academia.edu/Documents/in/Protein_Kinases?f_ri=55175"},{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=55175"},{"id":71471,"name":"Intestinal Mucosa","url":"https://www.academia.edu/Documents/in/Intestinal_Mucosa?f_ri=55175"},{"id":80715,"name":"ROOT","url":"https://www.academia.edu/Documents/in/ROOT?f_ri=55175"},{"id":83691,"name":"Ulcerative colitis","url":"https://www.academia.edu/Documents/in/Ulcerative_colitis?f_ri=55175"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=55175"},{"id":89805,"name":"Weight Loss","url":"https://www.academia.edu/Documents/in/Weight_Loss?f_ri=55175"},{"id":93922,"name":"Nitric oxide","url":"https://www.academia.edu/Documents/in/Nitric_oxide?f_ri=55175"},{"id":112334,"name":"Methanol","url":"https://www.academia.edu/Documents/in/Methanol?f_ri=55175"},{"id":123418,"name":"NF-kappa B","url":"https://www.academia.edu/Documents/in/NF-kappa_B?f_ri=55175"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=55175"},{"id":141395,"name":"Glutathione Peroxidase","url":"https://www.academia.edu/Documents/in/Glutathione_Peroxidase?f_ri=55175"},{"id":172083,"name":"Phosphorylation","url":"https://www.academia.edu/Documents/in/Phosphorylation?f_ri=55175"},{"id":202399,"name":"Plant Roots","url":"https://www.academia.edu/Documents/in/Plant_Roots?f_ri=55175"},{"id":204350,"name":"Anti-inflammatory agents","url":"https://www.academia.edu/Documents/in/Anti-inflammatory_agents?f_ri=55175"},{"id":208182,"name":"Anacardiaceae","url":"https://www.academia.edu/Documents/in/Anacardiaceae?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":213910,"name":"Mitogen Activated Protein Kinase","url":"https://www.academia.edu/Documents/in/Mitogen_Activated_Protein_Kinase?f_ri=55175"},{"id":246560,"name":"High Pressure Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Pressure_Liquid_Chromatography?f_ri=55175"},{"id":273338,"name":"IL","url":"https://www.academia.edu/Documents/in/IL?f_ri=55175"},{"id":317801,"name":"Cell nucleus","url":"https://www.academia.edu/Documents/in/Cell_nucleus?f_ri=55175"},{"id":335983,"name":"Lipopolysaccharides","url":"https://www.academia.edu/Documents/in/Lipopolysaccharides?f_ri=55175"},{"id":345219,"name":"Sedum","url":"https://www.academia.edu/Documents/in/Sedum?f_ri=55175"},{"id":345224,"name":"Lilium","url":"https://www.academia.edu/Documents/in/Lilium?f_ri=55175"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":382388,"name":"Nitric Oxide Synthase","url":"https://www.academia.edu/Documents/in/Nitric_Oxide_Synthase?f_ri=55175"},{"id":382481,"name":"Edema","url":"https://www.academia.edu/Documents/in/Edema?f_ri=55175"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=55175"},{"id":474029,"name":"Tumor necrosis factor-alpha","url":"https://www.academia.edu/Documents/in/Tumor_necrosis_factor-alpha?f_ri=55175"},{"id":545815,"name":"GeoDia","url":"https://www.academia.edu/Documents/in/GeoDia?f_ri=55175"},{"id":568887,"name":"Carrageenan","url":"https://www.academia.edu/Documents/in/Carrageenan?f_ri=55175"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=55175"},{"id":640273,"name":"Medicinal Food","url":"https://www.academia.edu/Documents/in/Medicinal_Food?f_ri=55175"},{"id":668178,"name":"Tnf alpha","url":"https://www.academia.edu/Documents/in/Tnf_alpha?f_ri=55175"},{"id":707585,"name":"Anti Inflammatory Activity","url":"https://www.academia.edu/Documents/in/Anti_Inflammatory_Activity?f_ri=55175"},{"id":789968,"name":"Extract","url":"https://www.academia.edu/Documents/in/Extract?f_ri=55175"},{"id":829416,"name":"Colon","url":"https://www.academia.edu/Documents/in/Colon?f_ri=55175"},{"id":841842,"name":"Lung Injury","url":"https://www.academia.edu/Documents/in/Lung_Injury?f_ri=55175"},{"id":1004785,"name":"Hemorrhage","url":"https://www.academia.edu/Documents/in/Hemorrhage?f_ri=55175"},{"id":1014763,"name":"Chlorophyta","url":"https://www.academia.edu/Documents/in/Chlorophyta?f_ri=55175"},{"id":1134083,"name":"Medicinal Plant","url":"https://www.academia.edu/Documents/in/Medicinal_Plant?f_ri=55175"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival?f_ri=55175"},{"id":1259531,"name":"Acute Appendicitis","url":"https://www.academia.edu/Documents/in/Acute_Appendicitis?f_ri=55175"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=55175"},{"id":1446339,"name":"Malondialdehyde","url":"https://www.academia.edu/Documents/in/Malondialdehyde?f_ri=55175"},{"id":1654024,"name":"Nitrites","url":"https://www.academia.edu/Documents/in/Nitrites?f_ri=55175"},{"id":1745595,"name":"Solvents","url":"https://www.academia.edu/Documents/in/Solvents?f_ri=55175"},{"id":1763968,"name":"Gene Expression Regulation","url":"https://www.academia.edu/Documents/in/Gene_Expression_Regulation?f_ri=55175"},{"id":1924712,"name":"Interleukin","url":"https://www.academia.edu/Documents/in/Interleukin?f_ri=55175"},{"id":2039739,"name":"Down-Regulation","url":"https://www.academia.edu/Documents/in/Down-Regulation?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_4975090" data-work_id="4975090" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/4975090/Evaluation_of_radish_Raphanus_sativus_L_peroxidase_activity_after_high_pressure_treatment_with_carbon_dioxide">Evaluation of radish ( Raphanus sativus L.) peroxidase activity after high-pressure treatment with carbon dioxide</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The objective of this work was to assess the influence of compressed CO 2 on the specific activity of peroxidase (POD) from radish (Raphanus sativus L.). The protein concentrate, in phosphate buffer, was obtained through the precipitation... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_4975090" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The objective of this work was to assess the influence of compressed CO 2 on the specific activity of peroxidase (POD) from radish (Raphanus sativus L.). The protein concentrate, in phosphate buffer, was obtained through the precipitation of proteins with (NH 4 ) 2 SO 4 . The peroxidase activity was determined using guaiacol as substrate. A semi-factorial experimental design was adopted to evaluate the effects of temperature (30-50 • C), exposure time (1-6 h), carbon dioxide density (ρ R = 0.6-1.8, resulting in pressures from 70.5 to 254.4 bar), and depressurization rate (10-200 kg/m 3 /min) on the peroxidase activity. Samples of lyophilized proteic concentrate were also submitted to high pressure and the effect of water content on the enzymatic activity in compressed CO 2 was studied. The water content in lyophilized samples was determined by thermogravimetric analysis. The results showed that the treatment of the enzymatic concentrate with CO 2 at high pressure could be a promising route to increase the POD activity.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4975090" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="589b8cf2f48bc474455d3cbc8c52fa90" rel="nofollow" data-download="{"attachment_id":49505247,"asset_id":4975090,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49505247/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="6568732" href="https://independent.academia.edu/deboraoliveira2">debora oliveira</a><script data-card-contents-for-user="6568732" type="text/json">{"id":6568732,"first_name":"debora","last_name":"oliveira","domain_name":"independent","page_name":"deboraoliveira2","display_name":"debora oliveira","profile_url":"https://independent.academia.edu/deboraoliveira2?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_4975090 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="4975090"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 4975090, container: ".js-paper-rank-work_4975090", }); });</script></li><li class="js-percentile-work_4975090 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 4975090; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_4975090"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_4975090 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="4975090"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 4975090; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=4975090]").text(description); $(".js-view-count-work_4975090").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_4975090").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="4975090"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" rel="nofollow" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4107" rel="nofollow" href="https://www.academia.edu/Documents/in/High_Pressure">High Pressure</a>, <script data-card-contents-for-ri="4107" type="text/json">{"id":4107,"name":"High Pressure","url":"https://www.academia.edu/Documents/in/High_Pressure?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4594" rel="nofollow" href="https://www.academia.edu/Documents/in/Carbon_Dioxide">Carbon Dioxide</a>, <script data-card-contents-for-ri="4594" type="text/json">{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9359" rel="nofollow" href="https://www.academia.edu/Documents/in/Supercritical_fluids">Supercritical fluids</a><script data-card-contents-for-ri="9359" type="text/json">{"id":9359,"name":"Supercritical fluids","url":"https://www.academia.edu/Documents/in/Supercritical_fluids?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=4975090]'), work: {"id":4975090,"title":"Evaluation of radish ( Raphanus sativus L.) peroxidase activity after high-pressure treatment with carbon dioxide","created_at":"2013-11-03T03:55:30.337-08:00","url":"https://www.academia.edu/4975090/Evaluation_of_radish_Raphanus_sativus_L_peroxidase_activity_after_high_pressure_treatment_with_carbon_dioxide?f_ri=55175","dom_id":"work_4975090","summary":"The objective of this work was to assess the influence of compressed CO 2 on the specific activity of peroxidase (POD) from radish (Raphanus sativus L.). The protein concentrate, in phosphate buffer, was obtained through the precipitation of proteins with (NH 4 ) 2 SO 4 . The peroxidase activity was determined using guaiacol as substrate. A semi-factorial experimental design was adopted to evaluate the effects of temperature (30-50 • C), exposure time (1-6 h), carbon dioxide density (ρ R = 0.6-1.8, resulting in pressures from 70.5 to 254.4 bar), and depressurization rate (10-200 kg/m 3 /min) on the peroxidase activity. Samples of lyophilized proteic concentrate were also submitted to high pressure and the effect of water content on the enzymatic activity in compressed CO 2 was studied. The water content in lyophilized samples was determined by thermogravimetric analysis. The results showed that the treatment of the enzymatic concentrate with CO 2 at high pressure could be a promising route to increase the POD activity.","downloadable_attachments":[{"id":49505247,"asset_id":4975090,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":6568732,"first_name":"debora","last_name":"oliveira","domain_name":"independent","page_name":"deboraoliveira2","display_name":"debora oliveira","profile_url":"https://independent.academia.edu/deboraoliveira2?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=55175","nofollow":true},{"id":4107,"name":"High Pressure","url":"https://www.academia.edu/Documents/in/High_Pressure?f_ri=55175","nofollow":true},{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide?f_ri=55175","nofollow":true},{"id":9359,"name":"Supercritical fluids","url":"https://www.academia.edu/Documents/in/Supercritical_fluids?f_ri=55175","nofollow":true},{"id":21747,"name":"Experimental Design","url":"https://www.academia.edu/Documents/in/Experimental_Design?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=55175"},{"id":161176,"name":"The","url":"https://www.academia.edu/Documents/in/The?f_ri=55175"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=55175"},{"id":399886,"name":"Specific Activity","url":"https://www.academia.edu/Documents/in/Specific_Activity?f_ri=55175"},{"id":845678,"name":"Water Content","url":"https://www.academia.edu/Documents/in/Water_Content?f_ri=55175"},{"id":868133,"name":"Raphanus sativus","url":"https://www.academia.edu/Documents/in/Raphanus_sativus?f_ri=55175"},{"id":1029284,"name":"Enzymatic Activity","url":"https://www.academia.edu/Documents/in/Enzymatic_Activity?f_ri=55175"},{"id":2281850,"name":"Thermo Gravimetric Analysis","url":"https://www.academia.edu/Documents/in/Thermo_Gravimetric_Analysis?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_7595334" data-work_id="7595334" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/7595334/Protective_effect_of_melatonin_against_the_inflammatory_response_elicited_by_crude_venom_from_isolated_nematocysts_of_Pelagia_noctiluca_Cnidaria_Scyphozoa">Protective effect of melatonin against the inflammatory response elicited by crude venom from isolated nematocysts of Pelagia noctiluca (Cnidaria, Scyphozoa</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Abstract: Melatonin (N-acetyl-5-methoxytryptamine) is an efficient free radical scavenger and antioxidant, both in vitro and in vivo. The role of melatonin as an immunomodulator is, in some cases, contradictory. In this study we have... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_7595334" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Abstract: Melatonin (N-acetyl-5-methoxytryptamine) is an efficient free radical scavenger and antioxidant, both in vitro and in vivo. The role of melatonin as an immunomodulator is, in some cases, contradictory. In this study we have investigated the therapeutic efficacy of melatonin in rats subjected to Pelagia noctiluca crude venom (of the familia Pelaguiidae; and genus Pelagia) induced acute paw inflammation. In particular, injection of the venom into the paw of rats elicited an acute inflammatory response characterized by accumulation of fluid containing a large number of polymorphonuclear neutrophils in the paw and subsequent lipid peroxidation. Furthermore, the venom promoted an expression of iNOS, nitrotyrosine and the activation of the nuclear enzyme poly (ADP-ribose) polymerase as determined by immunohistochemical analysis of paw tissues. Administration of melatonin 30 min, 1 and 6 hr after the challenge with the venom, caused a significant reduction in all the parameters of inflammation measured. Thus, based on these findings we propose that melatonin may be useful a treatment of local acute inflammation induced by P. noctiluca crude venom.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/7595334" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="72b880381a00c0da58d66cc70bb147a6" rel="nofollow" data-download="{"attachment_id":48405994,"asset_id":7595334,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48405994/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="13693739" href="https://unibas.academia.edu/RossanaMarino">Rossana Marino</a><script data-card-contents-for-user="13693739" type="text/json">{"id":13693739,"first_name":"Rossana","last_name":"Marino","domain_name":"unibas","page_name":"RossanaMarino","display_name":"Rossana Marino","profile_url":"https://unibas.academia.edu/RossanaMarino?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_7595334 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="7595334"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 7595334, container: ".js-paper-rank-work_7595334", }); });</script></li><li class="js-percentile-work_7595334 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 7595334; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_7595334"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_7595334 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="7595334"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 7595334; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=7595334]").text(description); $(".js-view-count-work_7595334").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_7595334").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="7595334"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2702" rel="nofollow" href="https://www.academia.edu/Documents/in/Immune_response">Immune response</a>, <script data-card-contents-for-ri="2702" type="text/json">{"id":2702,"name":"Immune response","url":"https://www.academia.edu/Documents/in/Immune_response?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9334" rel="nofollow" href="https://www.academia.edu/Documents/in/Inflammation">Inflammation</a>, <script data-card-contents-for-ri="9334" type="text/json">{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="24731" rel="nofollow" href="https://www.academia.edu/Documents/in/Apoptosis">Apoptosis</a>, <script data-card-contents-for-ri="24731" type="text/json">{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=7595334]'), work: {"id":7595334,"title":"Protective effect of melatonin against the inflammatory response elicited by crude venom from isolated nematocysts of Pelagia noctiluca (Cnidaria, Scyphozoa","created_at":"2014-07-08T01:41:20.995-07:00","url":"https://www.academia.edu/7595334/Protective_effect_of_melatonin_against_the_inflammatory_response_elicited_by_crude_venom_from_isolated_nematocysts_of_Pelagia_noctiluca_Cnidaria_Scyphozoa?f_ri=55175","dom_id":"work_7595334","summary":"Abstract: Melatonin (N-acetyl-5-methoxytryptamine) is an efficient free radical scavenger and antioxidant, both in vitro and in vivo. The role of melatonin as an immunomodulator is, in some cases, contradictory. In this study we have investigated the therapeutic efficacy of melatonin in rats subjected to Pelagia noctiluca crude venom (of the familia Pelaguiidae; and genus Pelagia) induced acute paw inflammation. In particular, injection of the venom into the paw of rats elicited an acute inflammatory response characterized by accumulation of fluid containing a large number of polymorphonuclear neutrophils in the paw and subsequent lipid peroxidation. Furthermore, the venom promoted an expression of iNOS, nitrotyrosine and the activation of the nuclear enzyme poly (ADP-ribose) polymerase as determined by immunohistochemical analysis of paw tissues. Administration of melatonin 30 min, 1 and 6 hr after the challenge with the venom, caused a significant reduction in all the parameters of inflammation measured. Thus, based on these findings we propose that melatonin may be useful a treatment of local acute inflammation induced by P. noctiluca crude venom.","downloadable_attachments":[{"id":48405994,"asset_id":7595334,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":13693739,"first_name":"Rossana","last_name":"Marino","domain_name":"unibas","page_name":"RossanaMarino","display_name":"Rossana Marino","profile_url":"https://unibas.academia.edu/RossanaMarino?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2702,"name":"Immune response","url":"https://www.academia.edu/Documents/in/Immune_response?f_ri=55175","nofollow":true},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=55175","nofollow":true},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":51570,"name":"Melatonin","url":"https://www.academia.edu/Documents/in/Melatonin?f_ri=55175"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175"},{"id":126465,"name":"Efficacy","url":"https://www.academia.edu/Documents/in/Efficacy?f_ri=55175"},{"id":204350,"name":"Anti-inflammatory agents","url":"https://www.academia.edu/Documents/in/Anti-inflammatory_agents?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":382481,"name":"Edema","url":"https://www.academia.edu/Documents/in/Edema?f_ri=55175"},{"id":432613,"name":"Animal models","url":"https://www.academia.edu/Documents/in/Animal_models?f_ri=55175"},{"id":545679,"name":"Scyphozoa","url":"https://www.academia.edu/Documents/in/Scyphozoa?f_ri=55175"},{"id":576476,"name":"Pineal","url":"https://www.academia.edu/Documents/in/Pineal?f_ri=55175"},{"id":2454817,"name":"Inflammatory response","url":"https://www.academia.edu/Documents/in/Inflammatory_response?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27935723" data-work_id="27935723" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27935723/Purification_of_peroxidase_isoenzymes_from_turnip_roots">Purification of peroxidase isoenzymes from turnip roots</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Simple reproducible procedures for purification of the main soluble (S) and ionically bound (IB) cationic peroxidase isoenzymes from turnip roots were established. The procedures included ammonium sulfate precipitation of the isoenzymes,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_27935723" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Simple reproducible procedures for purification of the main soluble (S) and ionically bound (IB) cationic peroxidase isoenzymes from turnip roots were established. The procedures included ammonium sulfate precipitation of the isoenzymes, chromatographic separation of the main isoenzymes using cellulose phosphate columns and purification to homogeneity by hydrophobic interaction chromatography on phenyl Sepharose columns. The specific activity of the phenyl Sepharose purified S and IB isoenzymes were 2760 and 896 units/mg protein with 140 and 4.8 fold increase over the crude extract and 38 and 13% recovery. The pH maxima and K,, for phenol and H20: of purified S and IB were determined.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27935723" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="10aabcdea701fef6c82109b912a52ced" rel="nofollow" data-download="{"attachment_id":48243466,"asset_id":27935723,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48243466/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="52346349" href="https://independent.academia.edu/RagaaHamed">Ragaah as Hamed</a><script data-card-contents-for-user="52346349" type="text/json">{"id":52346349,"first_name":"Ragaah as","last_name":"Hamed","domain_name":"independent","page_name":"RagaaHamed","display_name":"Ragaah as Hamed","profile_url":"https://independent.academia.edu/RagaaHamed?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_27935723 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="27935723"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 27935723, container: ".js-paper-rank-work_27935723", }); });</script></li><li class="js-percentile-work_27935723 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27935723; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_27935723"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_27935723 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="27935723"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27935723; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27935723]").text(description); $(".js-view-count-work_27935723").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_27935723").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="27935723"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3057" rel="nofollow" href="https://www.academia.edu/Documents/in/Phytochemistry">Phytochemistry</a>, <script data-card-contents-for-ri="3057" type="text/json">{"id":3057,"name":"Phytochemistry","url":"https://www.academia.edu/Documents/in/Phytochemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=27935723]'), work: {"id":27935723,"title":"Purification of peroxidase isoenzymes from turnip roots","created_at":"2016-08-22T01:52:30.307-07:00","url":"https://www.academia.edu/27935723/Purification_of_peroxidase_isoenzymes_from_turnip_roots?f_ri=55175","dom_id":"work_27935723","summary":"Simple reproducible procedures for purification of the main soluble (S) and ionically bound (IB) cationic peroxidase isoenzymes from turnip roots were established. The procedures included ammonium sulfate precipitation of the isoenzymes, chromatographic separation of the main isoenzymes using cellulose phosphate columns and purification to homogeneity by hydrophobic interaction chromatography on phenyl Sepharose columns. The specific activity of the phenyl Sepharose purified S and IB isoenzymes were 2760 and 896 units/mg protein with 140 and 4.8 fold increase over the crude extract and 38 and 13% recovery. The pH maxima and K,, for phenol and H20: of purified S and IB were determined.","downloadable_attachments":[{"id":48243466,"asset_id":27935723,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":52346349,"first_name":"Ragaah as","last_name":"Hamed","domain_name":"independent","page_name":"RagaaHamed","display_name":"Ragaah as Hamed","profile_url":"https://independent.academia.edu/RagaaHamed?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3057,"name":"Phytochemistry","url":"https://www.academia.edu/Documents/in/Phytochemistry?f_ri=55175","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":202399,"name":"Plant Roots","url":"https://www.academia.edu/Documents/in/Plant_Roots?f_ri=55175"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=55175"},{"id":376268,"name":"Brassica","url":"https://www.academia.edu/Documents/in/Brassica?f_ri=55175"},{"id":376284,"name":"Brassica rapa","url":"https://www.academia.edu/Documents/in/Brassica_rapa?f_ri=55175"},{"id":399886,"name":"Specific Activity","url":"https://www.academia.edu/Documents/in/Specific_Activity?f_ri=55175"},{"id":413115,"name":"Peroxidases","url":"https://www.academia.edu/Documents/in/Peroxidases?f_ri=55175"},{"id":432129,"name":"Substrate Specificity","url":"https://www.academia.edu/Documents/in/Substrate_Specificity?f_ri=55175"},{"id":437728,"name":"Isoenzymes","url":"https://www.academia.edu/Documents/in/Isoenzymes?f_ri=55175"},{"id":1363689,"name":"Ammonium Sulfate","url":"https://www.academia.edu/Documents/in/Ammonium_Sulfate?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27932195" data-work_id="27932195" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27932195/Drying_Characteristics_of_Red_Chilli">Drying Characteristics of Red Chilli</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27932195" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d2c3b45fe6a706aa1748a6f9f0b1d7f0" rel="nofollow" data-download="{"attachment_id":48238807,"asset_id":27932195,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48238807/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="52243525" href="https://independent.academia.edu/UShivhare">U. Shivhare</a><script data-card-contents-for-user="52243525" type="text/json">{"id":52243525,"first_name":"U.","last_name":"Shivhare","domain_name":"independent","page_name":"UShivhare","display_name":"U. Shivhare","profile_url":"https://independent.academia.edu/UShivhare?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_27932195 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="27932195"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 27932195, container: ".js-paper-rank-work_27932195", }); });</script></li><li class="js-percentile-work_27932195 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27932195; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_27932195"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_27932195 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="27932195"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27932195; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27932195]").text(description); $(".js-view-count-work_27932195").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_27932195").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="27932195"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="72" rel="nofollow" href="https://www.academia.edu/Documents/in/Chemical_Engineering">Chemical Engineering</a>, <script data-card-contents-for-ri="72" type="text/json">{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4188" rel="nofollow" href="https://www.academia.edu/Documents/in/Drying_Technology">Drying Technology</a>, <script data-card-contents-for-ri="4188" type="text/json">{"id":4188,"name":"Drying Technology","url":"https://www.academia.edu/Documents/in/Drying_Technology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="147640" rel="nofollow" href="https://www.academia.edu/Documents/in/Activation_Energy">Activation Energy</a><script data-card-contents-for-ri="147640" type="text/json">{"id":147640,"name":"Activation Energy","url":"https://www.academia.edu/Documents/in/Activation_Energy?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=27932195]'), work: {"id":27932195,"title":"Drying Characteristics of Red Chilli","created_at":"2016-08-21T21:33:00.761-07:00","url":"https://www.academia.edu/27932195/Drying_Characteristics_of_Red_Chilli?f_ri=55175","dom_id":"work_27932195","summary":null,"downloadable_attachments":[{"id":48238807,"asset_id":27932195,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":52243525,"first_name":"U.","last_name":"Shivhare","domain_name":"independent","page_name":"UShivhare","display_name":"U. Shivhare","profile_url":"https://independent.academia.edu/UShivhare?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=55175","nofollow":true},{"id":4188,"name":"Drying Technology","url":"https://www.academia.edu/Documents/in/Drying_Technology?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":147640,"name":"Activation Energy","url":"https://www.academia.edu/Documents/in/Activation_Energy?f_ri=55175","nofollow":true},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":329715,"name":"Product Quality","url":"https://www.academia.edu/Documents/in/Product_Quality?f_ri=55175"},{"id":391252,"name":"Room Temperature","url":"https://www.academia.edu/Documents/in/Room_Temperature?f_ri=55175"},{"id":444096,"name":"Air Temperature","url":"https://www.academia.edu/Documents/in/Air_Temperature?f_ri=55175"},{"id":585585,"name":"Food Additive","url":"https://www.academia.edu/Documents/in/Food_Additive?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9780024" data-work_id="9780024" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9780024/Involvement_of_polyamines_abscisic_acid_and_anti_oxidative_enzymes_in_adaptation_of_Blue_Panicgrass_Panicum_antidotale_Retz_to_saline_environments">Involvement of polyamines, abscisic acid and anti-oxidative enzymes in adaptation of Blue Panicgrass ( Panicum antidotale Retz.) to saline environments</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A hydroponic experiment was conducted to assess the possible involvement of polyamines (PAs), abscisic acid (ABA) and anti-oxidative enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in adaptation of six... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_9780024" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A hydroponic experiment was conducted to assess the possible involvement of polyamines (PAs), abscisic acid (ABA) and anti-oxidative enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in adaptation of six populations of Panicum antidotale Retz. to selection pressure (soil salinity) of a wide range of habitats. Plants of six populations were collected from six different habitats with ECe ranging from 3.39 to 19.23 dS m−1 and pH from 7.65 to 5.86. Young tillers from 6-month-old plants were transplanted in plastic containers each containing 10 l of half strength Hoagland's nutrient solution alone or with 150 mol m−3 NaCl. After 42 days growth, contents of polyamines (Put, Spd and Spm) and ABA, and the activities of anti-oxidative enzymes (SOD, POD and CAT) of all populations generally increased under salt stress. The populations collected from highly saline habitats showed a greater accumulation of polyamines and ABA and the activities of anti-oxidative enzymes as compared to those from mild or non-saline habitats. Moreover, Spm/Spd and Put/(Spd + Spm) ratios generally increased under salt stress. However, the populations from highly saline environments had significantly higher Spm/Spd and Put/(Spd + Spm) ratios as compared to those from mild or non-saline environments. Similarly, the populations adapted to high salinity accumulated less Na+ and Cl− in culm and leaves, and showed less decrease in leaf K+ and Ca2+ under salinity stress. Higher activities of anti-oxidative enzymes and accumulation of polyamines and ABA, and increased Spm/Spd and Put/(Spm + Spd) ratios were found to be highly correlated with the degree of adaptability of Panicum to saline environment.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9780024" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="736f76c6b475d4a0c363408db653181f" rel="nofollow" data-download="{"attachment_id":47649582,"asset_id":9780024,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47649582/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="23553976" href="https://independent.academia.edu/QasimAli48">Qasim Ali</a><script data-card-contents-for-user="23553976" type="text/json">{"id":23553976,"first_name":"Qasim","last_name":"Ali","domain_name":"independent","page_name":"QasimAli48","display_name":"Qasim Ali","profile_url":"https://independent.academia.edu/QasimAli48?f_ri=55175","photo":"https://0.academia-photos.com/23553976/6384136/7222834/s65_qasim.ali.jpg_oh_60ac01c267083d2b3ae402aeec01fc3b_oe_554451a7___gda___1430312155_85b3ad8f17d0690b764bf1b49f6bcce7"}</script></span></span></li><li class="js-paper-rank-work_9780024 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="9780024"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 9780024, container: ".js-paper-rank-work_9780024", }); });</script></li><li class="js-percentile-work_9780024 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 9780024; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_9780024"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_9780024 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="9780024"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 9780024; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=9780024]").text(description); $(".js-view-count-work_9780024").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_9780024").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="9780024"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="20285" rel="nofollow" href="https://www.academia.edu/Documents/in/Salt_Stress">Salt Stress</a>, <script data-card-contents-for-ri="20285" type="text/json">{"id":20285,"name":"Salt Stress","url":"https://www.academia.edu/Documents/in/Salt_Stress?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="26081" rel="nofollow" href="https://www.academia.edu/Documents/in/Soil_Salinity">Soil Salinity</a>, <script data-card-contents-for-ri="26081" type="text/json">{"id":26081,"name":"Soil Salinity","url":"https://www.academia.edu/Documents/in/Soil_Salinity?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=9780024]'), work: {"id":9780024,"title":"Involvement of polyamines, abscisic acid and anti-oxidative enzymes in adaptation of Blue Panicgrass ( Panicum antidotale Retz.) to saline environments","created_at":"2014-12-15T08:01:43.657-08:00","url":"https://www.academia.edu/9780024/Involvement_of_polyamines_abscisic_acid_and_anti_oxidative_enzymes_in_adaptation_of_Blue_Panicgrass_Panicum_antidotale_Retz_to_saline_environments?f_ri=55175","dom_id":"work_9780024","summary":"A hydroponic experiment was conducted to assess the possible involvement of polyamines (PAs), abscisic acid (ABA) and anti-oxidative enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in adaptation of six populations of Panicum antidotale Retz. to selection pressure (soil salinity) of a wide range of habitats. Plants of six populations were collected from six different habitats with ECe ranging from 3.39 to 19.23 dS m−1 and pH from 7.65 to 5.86. Young tillers from 6-month-old plants were transplanted in plastic containers each containing 10 l of half strength Hoagland's nutrient solution alone or with 150 mol m−3 NaCl. After 42 days growth, contents of polyamines (Put, Spd and Spm) and ABA, and the activities of anti-oxidative enzymes (SOD, POD and CAT) of all populations generally increased under salt stress. The populations collected from highly saline habitats showed a greater accumulation of polyamines and ABA and the activities of anti-oxidative enzymes as compared to those from mild or non-saline habitats. Moreover, Spm/Spd and Put/(Spd + Spm) ratios generally increased under salt stress. However, the populations from highly saline environments had significantly higher Spm/Spd and Put/(Spd + Spm) ratios as compared to those from mild or non-saline environments. Similarly, the populations adapted to high salinity accumulated less Na+ and Cl− in culm and leaves, and showed less decrease in leaf K+ and Ca2+ under salinity stress. Higher activities of anti-oxidative enzymes and accumulation of polyamines and ABA, and increased Spm/Spd and Put/(Spm + Spd) ratios were found to be highly correlated with the degree of adaptability of Panicum to saline environment.","downloadable_attachments":[{"id":47649582,"asset_id":9780024,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":23553976,"first_name":"Qasim","last_name":"Ali","domain_name":"independent","page_name":"QasimAli48","display_name":"Qasim Ali","profile_url":"https://independent.academia.edu/QasimAli48?f_ri=55175","photo":"https://0.academia-photos.com/23553976/6384136/7222834/s65_qasim.ali.jpg_oh_60ac01c267083d2b3ae402aeec01fc3b_oe_554451a7___gda___1430312155_85b3ad8f17d0690b764bf1b49f6bcce7"}],"research_interests":[{"id":20285,"name":"Salt Stress","url":"https://www.academia.edu/Documents/in/Salt_Stress?f_ri=55175","nofollow":true},{"id":26081,"name":"Soil Salinity","url":"https://www.academia.edu/Documents/in/Soil_Salinity?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=55175"},{"id":114835,"name":"Experimental Botany","url":"https://www.academia.edu/Documents/in/Experimental_Botany?f_ri=55175"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":798957,"name":"Salinity stress","url":"https://www.academia.edu/Documents/in/Salinity_stress?f_ri=55175"},{"id":1455911,"name":"Nutrient Solution","url":"https://www.academia.edu/Documents/in/Nutrient_Solution?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_11123882" data-work_id="11123882" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/11123882/Active_site_structure_of_the_catalase_peroxidases_from_Mycobacterium_tuberculosis_and_Escherichia_coli_by_extended_X_ray_absorption_fine_structure_analysis">Active site structure of the catalase-peroxidases from Mycobacterium tuberculosis and Escherichia coli by extended X-ray absorption fine structure analysis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The catalase-peroxidase encoded by katG of Mycobacterium tuberculosis is a more effective activator of the antibiotic isoniazid than is the equivalent enzyme from Escherichia coli. The environment of the heme iron was investigated using... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_11123882" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The catalase-peroxidase encoded by katG of Mycobacterium tuberculosis is a more effective activator of the antibiotic isoniazid than is the equivalent enzyme from Escherichia coli. The environment of the heme iron was investigated using X-ray absorption spectroscopy to determine if differences in this region were associated with the differences in reactivity. The variation in the distal side Fe^ligand distances between the two enzymes was the same within experimental error indicating that it was not the heme iron environment that produced the differences in reactivity. Analysis of variants of the E. coli catalase-peroxidase containing changes in active site residues Arg102 and His106 revealed small differences in Fe^water ligand distance including a shorter distance for the His106Tyr variant. The Arg102Leu variant was 5-coordinate, but His106Cys and Arg102Cys variants showed no changes within experimental error. These results are compared with those reported for other peroxidases. ß 2001 Elsevier Science B.V. All rights reserved.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/11123882" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e7d3f266cc878605de7d8081d33627e8" rel="nofollow" data-download="{"attachment_id":46879828,"asset_id":11123882,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46879828/download_file?st=MTczOTgwNTIzNSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="26865736" href="https://arizona.academia.edu/LindaPowers">Linda Powers</a><script data-card-contents-for-user="26865736" type="text/json">{"id":26865736,"first_name":"Linda","last_name":"Powers","domain_name":"arizona","page_name":"LindaPowers","display_name":"Linda Powers","profile_url":"https://arizona.academia.edu/LindaPowers?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_11123882 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="11123882"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 11123882, container: ".js-paper-rank-work_11123882", }); });</script></li><li class="js-percentile-work_11123882 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 11123882; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_11123882"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_11123882 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="11123882"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 11123882; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=11123882]").text(description); $(".js-view-count-work_11123882").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_11123882").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="11123882"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="12843" rel="nofollow" href="https://www.academia.edu/Documents/in/X_ray_absorption_spectroscopy">X ray absorption spectroscopy</a>, <script data-card-contents-for-ri="12843" type="text/json">{"id":12843,"name":"X ray absorption spectroscopy","url":"https://www.academia.edu/Documents/in/X_ray_absorption_spectroscopy?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="58906" rel="nofollow" href="https://www.academia.edu/Documents/in/Fourier_Analysis">Fourier Analysis</a><script data-card-contents-for-ri="58906" type="text/json">{"id":58906,"name":"Fourier Analysis","url":"https://www.academia.edu/Documents/in/Fourier_Analysis?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=11123882]'), work: {"id":11123882,"title":"Active site structure of the catalase-peroxidases from Mycobacterium tuberculosis and Escherichia coli by extended X-ray absorption fine structure analysis","created_at":"2015-02-26T14:31:20.504-08:00","url":"https://www.academia.edu/11123882/Active_site_structure_of_the_catalase_peroxidases_from_Mycobacterium_tuberculosis_and_Escherichia_coli_by_extended_X_ray_absorption_fine_structure_analysis?f_ri=55175","dom_id":"work_11123882","summary":"The catalase-peroxidase encoded by katG of Mycobacterium tuberculosis is a more effective activator of the antibiotic isoniazid than is the equivalent enzyme from Escherichia coli. The environment of the heme iron was investigated using X-ray absorption spectroscopy to determine if differences in this region were associated with the differences in reactivity. The variation in the distal side Fe^ligand distances between the two enzymes was the same within experimental error indicating that it was not the heme iron environment that produced the differences in reactivity. Analysis of variants of the E. coli catalase-peroxidase containing changes in active site residues Arg102 and His106 revealed small differences in Fe^water ligand distance including a shorter distance for the His106Tyr variant. The Arg102Leu variant was 5-coordinate, but His106Cys and Arg102Cys variants showed no changes within experimental error. These results are compared with those reported for other peroxidases. ß 2001 Elsevier Science B.V. All rights reserved.","downloadable_attachments":[{"id":46879828,"asset_id":11123882,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":26865736,"first_name":"Linda","last_name":"Powers","domain_name":"arizona","page_name":"LindaPowers","display_name":"Linda Powers","profile_url":"https://arizona.academia.edu/LindaPowers?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":12843,"name":"X ray absorption spectroscopy","url":"https://www.academia.edu/Documents/in/X_ray_absorption_spectroscopy?f_ri=55175","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":58906,"name":"Fourier Analysis","url":"https://www.academia.edu/Documents/in/Fourier_Analysis?f_ri=55175","nofollow":true},{"id":68318,"name":"Mycobacterium tuberculosis","url":"https://www.academia.edu/Documents/in/Mycobacterium_tuberculosis?f_ri=55175"},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation?f_ri=55175"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli?f_ri=55175"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=55175"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=55175"},{"id":158597,"name":"Iron","url":"https://www.academia.edu/Documents/in/Iron?f_ri=55175"},{"id":204375,"name":"Heme","url":"https://www.academia.edu/Documents/in/Heme?f_ri=55175"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=55175"},{"id":354019,"name":"Active site","url":"https://www.academia.edu/Documents/in/Active_site?f_ri=55175"},{"id":413115,"name":"Peroxidases","url":"https://www.academia.edu/Documents/in/Peroxidases?f_ri=55175"},{"id":905806,"name":"Electron Probe Microanalysis","url":"https://www.academia.edu/Documents/in/Electron_Probe_Microanalysis?f_ri=55175"},{"id":1217930,"name":"Site-directed Mutagenesis","url":"https://www.academia.edu/Documents/in/Site-directed_Mutagenesis?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14332130" data-work_id="14332130" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14332130/Diphenyl_diselenide_a_simple_glutathione_peroxidase_mimetic_inhibits_human_LDL_oxidation_in_vitro">Diphenyl diselenide, a simple glutathione peroxidase mimetic, inhibits human LDL oxidation in vitro</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Oxidative modification of low-density lipoprotein (LDL) represents an important factor in atherogenesis. In the present study, we have investigated the antioxidant capability of diphenyl diselenide (PhSe) 2 , a simple organoseleno... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14332130" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Oxidative modification of low-density lipoprotein (LDL) represents an important factor in atherogenesis. In the present study, we have investigated the antioxidant capability of diphenyl diselenide (PhSe) 2 , a simple organoseleno compound, against copper (Cu 2+ ) and peroxyl radical-induced human LDL oxidation in vitro. In initial studies using human serum, (PhSe) 2 caused a dose-dependent inhibition of Cu 2+induced lipid peroxidation, which was correlated to thiol consumption. (PhSe) 2 increased lipid peroxidation lag phase and decreased lipid peroxidation rate in isolated human LDL, evaluated by measuring both conjugated diene (CD) and thiobarbituric acid reactive substances (TBARS) levels. Consistent with these observations, (PhSe) 2 showed a marked inhibitory effect on 2,2-azobis(2-amidinopropane dihydrochloride) (AAPH)-induced oxidation of LDL or parinaric acid (PnA) incorporated into LDL. (PhSe) 2 also displayed a dose-dependent protective effect against Cu 2+ -induced lipid peroxidation in rat aortic slices. Interestingly, besides the antioxidant effects of (PhSe) 2 toward the lipid moieties of LDL, which was related to its thiol-peroxidase activity, protein moieties from human isolated LDL were also protected against Cu 2+ -induced oxidation. The results presented herein are the first to show that (i) (PhSe) 2 inhibits lipid peroxidation in human isolated LDL in vitro, (ii) this phenomenon is related to its thiol-peroxidase activity, and (iii) this chalcogen also prevents the oxidation of protein moieties of human LDL. Taken together, such data render (PhSe) 2 a promising molecule for pharmacological studies with respect to the atherogenic process.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14332130" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3541b63ec3ff1ff940aeb35559b6c13a" rel="nofollow" data-download="{"attachment_id":44313917,"asset_id":14332130,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44313917/download_file?st=MTczOTgwNTIzNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33280711" href="https://uc-pt.academia.edu/JLaranjinha">J. Laranjinha</a><script data-card-contents-for-user="33280711" type="text/json">{"id":33280711,"first_name":"J.","last_name":"Laranjinha","domain_name":"uc-pt","page_name":"JLaranjinha","display_name":"J. Laranjinha","profile_url":"https://uc-pt.academia.edu/JLaranjinha?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_14332130 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="14332130"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 14332130, container: ".js-paper-rank-work_14332130", }); });</script></li><li class="js-percentile-work_14332130 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 14332130; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_14332130"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_14332130 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="14332130"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 14332130; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=14332130]").text(description); $(".js-view-count-work_14332130").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_14332130").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="14332130"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="8015" rel="nofollow" href="https://www.academia.edu/Documents/in/Atherosclerosis">Atherosclerosis</a>, <script data-card-contents-for-ri="8015" type="text/json">{"id":8015,"name":"Atherosclerosis","url":"https://www.academia.edu/Documents/in/Atherosclerosis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14292" rel="nofollow" href="https://www.academia.edu/Documents/in/Oxidative_Stress">Oxidative Stress</a>, <script data-card-contents-for-ri="14292" type="text/json">{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="71510" rel="nofollow" href="https://www.academia.edu/Documents/in/Endothelial_Cells">Endothelial Cells</a><script data-card-contents-for-ri="71510" type="text/json">{"id":71510,"name":"Endothelial Cells","url":"https://www.academia.edu/Documents/in/Endothelial_Cells?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=14332130]'), work: {"id":14332130,"title":"Diphenyl diselenide, a simple glutathione peroxidase mimetic, inhibits human LDL oxidation in vitro","created_at":"2015-07-23T08:02:28.224-07:00","url":"https://www.academia.edu/14332130/Diphenyl_diselenide_a_simple_glutathione_peroxidase_mimetic_inhibits_human_LDL_oxidation_in_vitro?f_ri=55175","dom_id":"work_14332130","summary":"Oxidative modification of low-density lipoprotein (LDL) represents an important factor in atherogenesis. In the present study, we have investigated the antioxidant capability of diphenyl diselenide (PhSe) 2 , a simple organoseleno compound, against copper (Cu 2+ ) and peroxyl radical-induced human LDL oxidation in vitro. In initial studies using human serum, (PhSe) 2 caused a dose-dependent inhibition of Cu 2+induced lipid peroxidation, which was correlated to thiol consumption. (PhSe) 2 increased lipid peroxidation lag phase and decreased lipid peroxidation rate in isolated human LDL, evaluated by measuring both conjugated diene (CD) and thiobarbituric acid reactive substances (TBARS) levels. Consistent with these observations, (PhSe) 2 showed a marked inhibitory effect on 2,2-azobis(2-amidinopropane dihydrochloride) (AAPH)-induced oxidation of LDL or parinaric acid (PnA) incorporated into LDL. (PhSe) 2 also displayed a dose-dependent protective effect against Cu 2+ -induced lipid peroxidation in rat aortic slices. Interestingly, besides the antioxidant effects of (PhSe) 2 toward the lipid moieties of LDL, which was related to its thiol-peroxidase activity, protein moieties from human isolated LDL were also protected against Cu 2+ -induced oxidation. The results presented herein are the first to show that (i) (PhSe) 2 inhibits lipid peroxidation in human isolated LDL in vitro, (ii) this phenomenon is related to its thiol-peroxidase activity, and (iii) this chalcogen also prevents the oxidation of protein moieties of human LDL. Taken together, such data render (PhSe) 2 a promising molecule for pharmacological studies with respect to the atherogenic process.","downloadable_attachments":[{"id":44313917,"asset_id":14332130,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33280711,"first_name":"J.","last_name":"Laranjinha","domain_name":"uc-pt","page_name":"JLaranjinha","display_name":"J. Laranjinha","profile_url":"https://uc-pt.academia.edu/JLaranjinha?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":8015,"name":"Atherosclerosis","url":"https://www.academia.edu/Documents/in/Atherosclerosis?f_ri=55175","nofollow":true},{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":71510,"name":"Endothelial Cells","url":"https://www.academia.edu/Documents/in/Endothelial_Cells?f_ri=55175","nofollow":true},{"id":80692,"name":"Copper","url":"https://www.academia.edu/Documents/in/Copper?f_ri=55175"},{"id":95519,"name":"Selenium","url":"https://www.academia.edu/Documents/in/Selenium?f_ri=55175"},{"id":141395,"name":"Glutathione Peroxidase","url":"https://www.academia.edu/Documents/in/Glutathione_Peroxidase?f_ri=55175"},{"id":228670,"name":"Organoselenium Compounds","url":"https://www.academia.edu/Documents/in/Organoselenium_Compounds?f_ri=55175"},{"id":233372,"name":"Lipid peroxidation","url":"https://www.academia.edu/Documents/in/Lipid_peroxidation?f_ri=55175"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=55175"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=55175"},{"id":413115,"name":"Peroxidases","url":"https://www.academia.edu/Documents/in/Peroxidases?f_ri=55175"},{"id":563382,"name":"Oxidation","url":"https://www.academia.edu/Documents/in/Oxidation?f_ri=55175"},{"id":563659,"name":"Human Serum","url":"https://www.academia.edu/Documents/in/Human_Serum?f_ri=55175"},{"id":564879,"name":"Wistar Rats","url":"https://www.academia.edu/Documents/in/Wistar_Rats?f_ri=55175"},{"id":1035092,"name":"Aorta","url":"https://www.academia.edu/Documents/in/Aorta?f_ri=55175"},{"id":1244809,"name":"Thiobarbituric Acid Reactive Substance","url":"https://www.academia.edu/Documents/in/Thiobarbituric_Acid_Reactive_Substance?f_ri=55175"},{"id":1274496,"name":"Low Density Lipoprotein (LDL)","url":"https://www.academia.edu/Documents/in/Low_Density_Lipoprotein_LDL_?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15560249" data-work_id="15560249" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15560249/Antifibrotic_effect_of_decorin_in_a_bleomycin_hamster_model_of_lung_fibrosis">Antifibrotic effect of decorin in a bleomycin hamster model of lung fibrosis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We reported previously that treatment with antibody to transforming growth factor-p (TGF-B) caused a marked attenuation of bleomycin (BL)-induced lung fibrosis (LF) in mice. Decorin (DC), a proteoglycan, binds TGF-B and thereby... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15560249" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We reported previously that treatment with antibody to transforming growth factor-p (TGF-B) caused a marked attenuation of bleomycin (BL)-induced lung fibrosis (LF) in mice. Decorin (DC), a proteoglycan, binds TGF-B and thereby down-regulates all of its biological activities. In the present study, we evaluated the antifibrotic potential of DC in a three-dose BL-hamster mode1 of lung fibrosis. Hamsters were placed in the following groups: (1) saline (SA) + phosphate-buffered saline (PBS) (SA + PBS); (2) SA + DC; (3) BL + PBS; and (4) BL + DC. Under pentobarbital anesthesia, SA (4 mL/kg) or BL was instilled intratracheally in three consecutive doses (2.5, 2.0, 1.5 units/kg/4 mL) at weekly intervals. DC (1 mg/mL) or PBS was instilled intratracheally in 0.4 mL/hamster on days 3 and 5 foll owing instillation of each dose of SA or BL. In week 4, hamsters received three doses of either DC or PBS every other day. The hamsters were killed at 30 days following the first instillation, and their lungs were appropriately processed. Lung hydroxyproline levels in SA + PBS, SA + DC, BL + PBS, and BL + DC groups were 965, 829, 1854, and 1387 p,g/lung, respectively. Prolyl hydroxylase activities were 103, 289, and 193% of SA + PBS control in SA + DC, BL + PBS, and BL + DC groups, respectively. The myeloperoxidase activities in the corresponding groups were 222, 890, and 274% of control (0.525 units/lung). Intratracheal instillation of BL caused significant increases in these biochemical markers, and instillation of DC diminished these increases in the BL + DC group. DC treatment also caused a significant reduction in the infiltration of neutrophils in the bronchoalveolar lavage fluid (BALF) of hamsters in the BL + DC group. However, DC treatment had little effect on BL-induced increases in lung superoxide dismutase activity and lipid peroxidation and leakage of plasma proteins in the BALF of the BL + DC group. Hamsters in the BL + PBS group showed severe multifocal fibrosis and accumulation of mononuclear inflammatory cells and granulocytes. In contrast, hamsters in the BL + DC group showed mild multifocal septal thickening with aggregations of mononuclear inflammatory cells. Hamsters in both control groups (SA + PBS</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15560249" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3f85c7d2326b36480e07d393bb4a1dd8" rel="nofollow" data-download="{"attachment_id":43084592,"asset_id":15560249,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43084592/download_file?st=MTczOTgwNTIzNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34720448" href="https://ucdavis.academia.edu/ShriGiri">Shri Giri</a><script data-card-contents-for-user="34720448" type="text/json">{"id":34720448,"first_name":"Shri","last_name":"Giri","domain_name":"ucdavis","page_name":"ShriGiri","display_name":"Shri Giri","profile_url":"https://ucdavis.academia.edu/ShriGiri?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_15560249 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="15560249"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 15560249, container: ".js-paper-rank-work_15560249", }); });</script></li><li class="js-percentile-work_15560249 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15560249; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_15560249"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_15560249 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="15560249"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15560249; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15560249]").text(description); $(".js-view-count-work_15560249").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_15560249").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="15560249"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="12071" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a>, <script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="71308" rel="nofollow" href="https://www.academia.edu/Documents/in/Pulmonary_fibrosis">Pulmonary fibrosis</a>, <script data-card-contents-for-ri="71308" type="text/json">{"id":71308,"name":"Pulmonary fibrosis","url":"https://www.academia.edu/Documents/in/Pulmonary_fibrosis?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="176525" rel="nofollow" href="https://www.academia.edu/Documents/in/Biochemical">Biochemical</a><script data-card-contents-for-ri="176525" type="text/json">{"id":176525,"name":"Biochemical","url":"https://www.academia.edu/Documents/in/Biochemical?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=15560249]'), work: {"id":15560249,"title":"Antifibrotic effect of decorin in a bleomycin hamster model of lung fibrosis","created_at":"2015-09-09T19:29:59.426-07:00","url":"https://www.academia.edu/15560249/Antifibrotic_effect_of_decorin_in_a_bleomycin_hamster_model_of_lung_fibrosis?f_ri=55175","dom_id":"work_15560249","summary":"We reported previously that treatment with antibody to transforming growth factor-p (TGF-B) caused a marked attenuation of bleomycin (BL)-induced lung fibrosis (LF) in mice. Decorin (DC), a proteoglycan, binds TGF-B and thereby down-regulates all of its biological activities. In the present study, we evaluated the antifibrotic potential of DC in a three-dose BL-hamster mode1 of lung fibrosis. Hamsters were placed in the following groups: (1) saline (SA) + phosphate-buffered saline (PBS) (SA + PBS); (2) SA + DC; (3) BL + PBS; and (4) BL + DC. Under pentobarbital anesthesia, SA (4 mL/kg) or BL was instilled intratracheally in three consecutive doses (2.5, 2.0, 1.5 units/kg/4 mL) at weekly intervals. DC (1 mg/mL) or PBS was instilled intratracheally in 0.4 mL/hamster on days 3 and 5 foll owing instillation of each dose of SA or BL. In week 4, hamsters received three doses of either DC or PBS every other day. The hamsters were killed at 30 days following the first instillation, and their lungs were appropriately processed. Lung hydroxyproline levels in SA + PBS, SA + DC, BL + PBS, and BL + DC groups were 965, 829, 1854, and 1387 p,g/lung, respectively. Prolyl hydroxylase activities were 103, 289, and 193% of SA + PBS control in SA + DC, BL + PBS, and BL + DC groups, respectively. The myeloperoxidase activities in the corresponding groups were 222, 890, and 274% of control (0.525 units/lung). Intratracheal instillation of BL caused significant increases in these biochemical markers, and instillation of DC diminished these increases in the BL + DC group. DC treatment also caused a significant reduction in the infiltration of neutrophils in the bronchoalveolar lavage fluid (BALF) of hamsters in the BL + DC group. However, DC treatment had little effect on BL-induced increases in lung superoxide dismutase activity and lipid peroxidation and leakage of plasma proteins in the BALF of the BL + DC group. Hamsters in the BL + PBS group showed severe multifocal fibrosis and accumulation of mononuclear inflammatory cells and granulocytes. In contrast, hamsters in the BL + DC group showed mild multifocal septal thickening with aggregations of mononuclear inflammatory cells. Hamsters in both control groups (SA + PBS","downloadable_attachments":[{"id":43084592,"asset_id":15560249,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34720448,"first_name":"Shri","last_name":"Giri","domain_name":"ucdavis","page_name":"ShriGiri","display_name":"Shri Giri","profile_url":"https://ucdavis.academia.edu/ShriGiri?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":71308,"name":"Pulmonary fibrosis","url":"https://www.academia.edu/Documents/in/Pulmonary_fibrosis?f_ri=55175","nofollow":true},{"id":176525,"name":"Biochemical","url":"https://www.academia.edu/Documents/in/Biochemical?f_ri=55175","nofollow":true},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=55175"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=55175"},{"id":350927,"name":"Proteoglycans","url":"https://www.academia.edu/Documents/in/Proteoglycans?f_ri=55175"},{"id":371048,"name":"Lung fibrosis","url":"https://www.academia.edu/Documents/in/Lung_fibrosis?f_ri=55175"},{"id":487159,"name":"Group","url":"https://www.academia.edu/Documents/in/Group?f_ri=55175"},{"id":527900,"name":"Transforming Growth Factor Beta","url":"https://www.academia.edu/Documents/in/Transforming_Growth_Factor_Beta?f_ri=55175"},{"id":648326,"name":"BIOCHEMICAL PHARMACOLOGY","url":"https://www.academia.edu/Documents/in/BIOCHEMICAL_PHARMACOLOGY?f_ri=55175"},{"id":695493,"name":"Bleomycin","url":"https://www.academia.edu/Documents/in/Bleomycin?f_ri=55175"},{"id":783432,"name":"Biological activity","url":"https://www.academia.edu/Documents/in/Biological_activity?f_ri=55175"},{"id":802828,"name":"Hydroxyproline","url":"https://www.academia.edu/Documents/in/Hydroxyproline?f_ri=55175"},{"id":951344,"name":"Growth Factor","url":"https://www.academia.edu/Documents/in/Growth_Factor?f_ri=55175"},{"id":1127159,"name":"Decorin","url":"https://www.academia.edu/Documents/in/Decorin?f_ri=55175"},{"id":1401310,"name":"Myeloperoxidase","url":"https://www.academia.edu/Documents/in/Myeloperoxidase?f_ri=55175"},{"id":1631043,"name":"Control Group","url":"https://www.academia.edu/Documents/in/Control_Group?f_ri=55175"},{"id":1817281,"name":"Extracellular Matrix Proteins","url":"https://www.academia.edu/Documents/in/Extracellular_Matrix_Proteins?f_ri=55175"},{"id":2273474,"name":"Transforming Growth Factor","url":"https://www.academia.edu/Documents/in/Transforming_Growth_Factor?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19219747" data-work_id="19219747" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19219747/Ascorbate_and_glutathione_guardians_of_the_cell_cycle_partners_in_crime">Ascorbate and glutathione: guardians of the cell cycle, partners in crime?</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Besides the implication of ascorbate and glutathione in the defence against oxidative stress, these two compounds are involved in plant growth and cell cycle control. Ascorbate metabolism is closely linked to the development of embryos... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19219747" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Besides the implication of ascorbate and glutathione in the defence against oxidative stress, these two compounds are involved in plant growth and cell cycle control. Ascorbate metabolism is closely linked to the development of embryos and seedlings. Furthermore, ascorbate stimulates cell cycle activity in competent cells, while the oxidised form, dehydroascorbate, blocks normal cell cycle progression. Several possible mechanisms have been proposed to explain the effect of these compounds. The links between glutathione and the cell cycle are less clear. It has long been assumed that both compounds are closely linked by way of the Halliwell-Asada cycle. Any hypothesis concerning the pathways by which ascorbate or glutathione influence cell division, should take this connection into account. However, other mechanisms have been proposed for ascorbate-mediated cell cycle control, e.g. via the thioredoxin pathway.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19219747" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0d0d03d3a0fca9ad4349f30dc522bff4" rel="nofollow" data-download="{"attachment_id":40498275,"asset_id":19219747,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40498275/download_file?st=MTczOTgwNTIzNiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39447979" href="https://sckcen.academia.edu/NeleHoremans">Nele Horemans</a><script data-card-contents-for-user="39447979" type="text/json">{"id":39447979,"first_name":"Nele","last_name":"Horemans","domain_name":"sckcen","page_name":"NeleHoremans","display_name":"Nele Horemans","profile_url":"https://sckcen.academia.edu/NeleHoremans?f_ri=55175","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_19219747 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19219747"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19219747, container: ".js-paper-rank-work_19219747", }); });</script></li><li class="js-percentile-work_19219747 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 19219747; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_19219747"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_19219747 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="19219747"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 19219747; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=19219747]").text(description); $(".js-view-count-work_19219747").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_19219747").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="19219747"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9113" rel="nofollow" href="https://www.academia.edu/Documents/in/Cell_Cycle">Cell Cycle</a>, <script data-card-contents-for-ri="9113" type="text/json">{"id":9113,"name":"Cell Cycle","url":"https://www.academia.edu/Documents/in/Cell_Cycle?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="38650" rel="nofollow" href="https://www.academia.edu/Documents/in/Cell_Division">Cell Division</a>, <script data-card-contents-for-ri="38650" type="text/json">{"id":38650,"name":"Cell Division","url":"https://www.academia.edu/Documents/in/Cell_Division?f_ri=55175","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55175" rel="nofollow" href="https://www.academia.edu/Documents/in/Peroxidase">Peroxidase</a><script data-card-contents-for-ri="55175" type="text/json">{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19219747]'), work: {"id":19219747,"title":"Ascorbate and glutathione: guardians of the cell cycle, partners in crime?","created_at":"2015-11-30T02:23:46.306-08:00","url":"https://www.academia.edu/19219747/Ascorbate_and_glutathione_guardians_of_the_cell_cycle_partners_in_crime?f_ri=55175","dom_id":"work_19219747","summary":"Besides the implication of ascorbate and glutathione in the defence against oxidative stress, these two compounds are involved in plant growth and cell cycle control. Ascorbate metabolism is closely linked to the development of embryos and seedlings. Furthermore, ascorbate stimulates cell cycle activity in competent cells, while the oxidised form, dehydroascorbate, blocks normal cell cycle progression. Several possible mechanisms have been proposed to explain the effect of these compounds. The links between glutathione and the cell cycle are less clear. It has long been assumed that both compounds are closely linked by way of the Halliwell-Asada cycle. Any hypothesis concerning the pathways by which ascorbate or glutathione influence cell division, should take this connection into account. However, other mechanisms have been proposed for ascorbate-mediated cell cycle control, e.g. via the thioredoxin pathway.","downloadable_attachments":[{"id":40498275,"asset_id":19219747,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39447979,"first_name":"Nele","last_name":"Horemans","domain_name":"sckcen","page_name":"NeleHoremans","display_name":"Nele Horemans","profile_url":"https://sckcen.academia.edu/NeleHoremans?f_ri=55175","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=55175","nofollow":true},{"id":9113,"name":"Cell Cycle","url":"https://www.academia.edu/Documents/in/Cell_Cycle?f_ri=55175","nofollow":true},{"id":38650,"name":"Cell Division","url":"https://www.academia.edu/Documents/in/Cell_Division?f_ri=55175","nofollow":true},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=55175","nofollow":true},{"id":118450,"name":"Glutathione","url":"https://www.academia.edu/Documents/in/Glutathione?f_ri=55175"},{"id":127006,"name":"Plant growth","url":"https://www.academia.edu/Documents/in/Plant_growth?f_ri=55175"},{"id":217324,"name":"Soil sciences","url":"https://www.academia.edu/Documents/in/Soil_sciences?f_ri=55175"},{"id":241324,"name":"Plant Physiology and Biochemistry","url":"https://www.academia.edu/Documents/in/Plant_Physiology_and_Biochemistry?f_ri=55175"},{"id":352757,"name":"Ascorbic Acid","url":"https://www.academia.edu/Documents/in/Ascorbic_Acid?f_ri=55175"},{"id":364039,"name":"Thioredoxin","url":"https://www.academia.edu/Documents/in/Thioredoxin?f_ri=55175"},{"id":1027717,"name":"Embryos","url":"https://www.academia.edu/Documents/in/Embryos?f_ri=55175"},{"id":1353990,"name":"Cell Cycle Control","url":"https://www.academia.edu/Documents/in/Cell_Cycle_Control?f_ri=55175"}]}, }) } })();</script></ul></li></ul></div></div></div><div class="u-taCenter Pagination"><ul class="pagination"><li class="next_page"><a href="/Documents/in/Peroxidase?after=50%2C19219747" rel="next">Next</a></li><li class="last next"><a href="/Documents/in/Peroxidase?page=last">Last »</a></li></ul></div></div><div class="hidden-xs hidden-sm"><div class="u-pl6x"><div style="width: 300px;"><div class="panel panel-flat u-mt7x"><div class="panel-heading u-p5x"><div class="u-tcGrayDark u-taCenter u-fw700 u-textUppercase">Related Topics</div></div><ul class="list-group"><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Aerosols">Aerosols</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="36763">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="36763">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Nitrite">Nitrite</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="162978">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="162978">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Superoxide_Dismutase">Superoxide Dismutase</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="213343">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="213343">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Catalase">Catalase</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="139007">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="139007">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Permeability">Permeability</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="83972">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="83972">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Hydrogen_Peroxide">Hydrogen Peroxide</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="274826">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="274826">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Streptomyces">Streptomyces</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="55172">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="55172">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Glutathione">Glutathione</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="118450">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="118450">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Enzyme">Enzyme</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="231661">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="231661">Following</a></div></li><li class="list-group-item media_v2 u-mt0x u-p3x"><div class="media-body"><div class="u-tcGrayDarker u-fw700"><a class="u-tcGrayDarker" rel="nofollow" href="https://www.academia.edu/Documents/in/Enzyme_activity">Enzyme activity</a></div></div><div class="media-right media-middle"><a class="u-tcGreen u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-follow-ri-id="583568">Follow</a><a class="u-tcGray u-textDecorationNone u-linkUnstyled u-fw500 hidden" data-unfollow-ri-id="583568">Following</a></div></li></ul></div></div></div></div></div></div><script>// MIT License // Copyright © 2011 Sebastian Tschan, https://blueimp.net // Permission is hereby granted, free of charge, to any person obtaining a copy of // this software and associated documentation files (the "Software"), to deal in // the Software without restriction, including without limitation the rights to // use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of // the Software, and to permit persons to whom the Software is furnished to do so, // subject to the following conditions: // The above copyright notice and this permission notice shall be included in all // copies or substantial portions of the Software. // THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR // IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS // FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR // COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER // IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN // CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. !function(n){"use strict";function d(n,t){var r=(65535&n)+(65535&t);return(n>>16)+(t>>16)+(r>>16)<<16|65535&r}function f(n,t,r,e,o,u){return d((c=d(d(t,n),d(e,u)))<<(f=o)|c>>>32-f,r);var c,f}function l(n,t,r,e,o,u,c){return f(t&r|~t&e,n,t,o,u,c)}function v(n,t,r,e,o,u,c){return f(t&e|r&~e,n,t,o,u,c)}function g(n,t,r,e,o,u,c){return f(t^r^e,n,t,o,u,c)}function m(n,t,r,e,o,u,c){return f(r^(t|~e),n,t,o,u,c)}function i(n,t){var r,e,o,u;n[t>>5]|=128<<t%32,n[14+(t+64>>>9<<4)]=t;for(var c=1732584193,f=-271733879,i=-1732584194,a=271733878,h=0;h<n.length;h+=16)c=l(r=c,e=f,o=i,u=a,n[h],7,-680876936),a=l(a,c,f,i,n[h+1],12,-389564586),i=l(i,a,c,f,n[h+2],17,606105819),f=l(f,i,a,c,n[h+3],22,-1044525330),c=l(c,f,i,a,n[h+4],7,-176418897),a=l(a,c,f,i,n[h+5],12,1200080426),i=l(i,a,c,f,n[h+6],17,-1473231341),f=l(f,i,a,c,n[h+7],22,-45705983),c=l(c,f,i,a,n[h+8],7,1770035416),a=l(a,c,f,i,n[h+9],12,-1958414417),i=l(i,a,c,f,n[h+10],17,-42063),f=l(f,i,a,c,n[h+11],22,-1990404162),c=l(c,f,i,a,n[h+12],7,1804603682),a=l(a,c,f,i,n[h+13],12,-40341101),i=l(i,a,c,f,n[h+14],17,-1502002290),c=v(c,f=l(f,i,a,c,n[h+15],22,1236535329),i,a,n[h+1],5,-165796510),a=v(a,c,f,i,n[h+6],9,-1069501632),i=v(i,a,c,f,n[h+11],14,643717713),f=v(f,i,a,c,n[h],20,-373897302),c=v(c,f,i,a,n[h+5],5,-701558691),a=v(a,c,f,i,n[h+10],9,38016083),i=v(i,a,c,f,n[h+15],14,-660478335),f=v(f,i,a,c,n[h+4],20,-405537848),c=v(c,f,i,a,n[h+9],5,568446438),a=v(a,c,f,i,n[h+14],9,-1019803690),i=v(i,a,c,f,n[h+3],14,-187363961),f=v(f,i,a,c,n[h+8],20,1163531501),c=v(c,f,i,a,n[h+13],5,-1444681467),a=v(a,c,f,i,n[h+2],9,-51403784),i=v(i,a,c,f,n[h+7],14,1735328473),c=g(c,f=v(f,i,a,c,n[h+12],20,-1926607734),i,a,n[h+5],4,-378558),a=g(a,c,f,i,n[h+8],11,-2022574463),i=g(i,a,c,f,n[h+11],16,1839030562),f=g(f,i,a,c,n[h+14],23,-35309556),c=g(c,f,i,a,n[h+1],4,-1530992060),a=g(a,c,f,i,n[h+4],11,1272893353),i=g(i,a,c,f,n[h+7],16,-155497632),f=g(f,i,a,c,n[h+10],23,-1094730640),c=g(c,f,i,a,n[h+13],4,681279174),a=g(a,c,f,i,n[h],11,-358537222),i=g(i,a,c,f,n[h+3],16,-722521979),f=g(f,i,a,c,n[h+6],23,76029189),c=g(c,f,i,a,n[h+9],4,-640364487),a=g(a,c,f,i,n[h+12],11,-421815835),i=g(i,a,c,f,n[h+15],16,530742520),c=m(c,f=g(f,i,a,c,n[h+2],23,-995338651),i,a,n[h],6,-198630844),a=m(a,c,f,i,n[h+7],10,1126891415),i=m(i,a,c,f,n[h+14],15,-1416354905),f=m(f,i,a,c,n[h+5],21,-57434055),c=m(c,f,i,a,n[h+12],6,1700485571),a=m(a,c,f,i,n[h+3],10,-1894986606),i=m(i,a,c,f,n[h+10],15,-1051523),f=m(f,i,a,c,n[h+1],21,-2054922799),c=m(c,f,i,a,n[h+8],6,1873313359),a=m(a,c,f,i,n[h+15],10,-30611744),i=m(i,a,c,f,n[h+6],15,-1560198380),f=m(f,i,a,c,n[h+13],21,1309151649),c=m(c,f,i,a,n[h+4],6,-145523070),a=m(a,c,f,i,n[h+11],10,-1120210379),i=m(i,a,c,f,n[h+2],15,718787259),f=m(f,i,a,c,n[h+9],21,-343485551),c=d(c,r),f=d(f,e),i=d(i,o),a=d(a,u);return[c,f,i,a]}function a(n){for(var t="",r=32*n.length,e=0;e<r;e+=8)t+=String.fromCharCode(n[e>>5]>>>e%32&255);return t}function h(n){var t=[];for(t[(n.length>>2)-1]=void 0,e=0;e<t.length;e+=1)t[e]=0;for(var r=8*n.length,e=0;e<r;e+=8)t[e>>5]|=(255&n.charCodeAt(e/8))<<e%32;return t}function e(n){for(var t,r="0123456789abcdef",e="",o=0;o<n.length;o+=1)t=n.charCodeAt(o),e+=r.charAt(t>>>4&15)+r.charAt(15&t);return e}function r(n){return unescape(encodeURIComponent(n))}function o(n){return a(i(h(t=r(n)),8*t.length));var t}function u(n,t){return function(n,t){var r,e,o=h(n),u=[],c=[];for(u[15]=c[15]=void 0,16<o.length&&(o=i(o,8*n.length)),r=0;r<16;r+=1)u[r]=909522486^o[r],c[r]=1549556828^o[r];return e=i(u.concat(h(t)),512+8*t.length),a(i(c.concat(e),640))}(r(n),r(t))}function t(n,t,r){return t?r?u(t,n):e(u(t,n)):r?o(n):e(o(n))}"function"==typeof define&&define.amd?define(function(){return t}):"object"==typeof module&&module.exports?module.exports=t:n.md5=t}(this);</script><script>window.AbTest = (function() { return { 'ab_test': (uniqueId, test_name, buckets) => { let override = new URLSearchParams(window.location.search).get(`ab_test[${test_name}]`); if ( override ) { return override; } const bucketNames = buckets.map((bucket) => { return typeof bucket === 'string' ? bucket : Object.keys(bucket)[0]; }); const weights = buckets.map((bucket) => { return typeof bucket === 'string' ? 1 : Object.values(bucket)[0]; }); const total = weights.reduce((sum, weight) => sum + weight); const hash = md5(`${uniqueId}${test_name}`); const hashNum = parseInt(hash.slice(-12), 16); let bucketPoint = total * (hashNum % 100000) / 100000; const bucket = bucketNames.find((_, i) => { if (weights[i] > bucketPoint) { return true; } bucketPoint -= weights[i]; return false; }); return bucket; } }; })();</script><div data-auto_select="false" data-client_id="331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b" data-landing_url="https://www.academia.edu/Documents/in/Peroxidase" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><script>function onGoogleOneTapEvent(event) { var momentType = event.getMomentType(); var momentReason = null; if (event.isNotDisplayed()) { momentReason = event.getNotDisplayedReason(); } else if (event.isSkippedMoment()) { momentReason = event.getSkippedReason(); } else if (event.isDismissedMoment()) { momentReason = event.getDismissedReason(); } Aedu.arbitraryEvents.write('GoogleOneTapEvent', { moment_type: momentType, moment_reason: momentReason, }); }</script><script>(function() { var auvid = unescape( document.cookie .split(/; ?/) .find((s) => s.startsWith('auvid')) .substring(6)); var bucket = AbTest.ab_test(auvid, 'lo_ri_one_tap_google_sign_on', ['control', 'one_tap_google_sign_on']); if (bucket === 'control') return; var oneTapTag = document.createElement('script') oneTapTag.async = true oneTapTag.defer = true oneTapTag.src = 'https://accounts.google.com/gsi/client' document.body.appendChild(oneTapTag) })();</script></div></div></div> </div> <div class="bootstrap login"><div class="modal fade login-modal" id="login-modal"><div class="login-modal-dialog modal-dialog"><div class="modal-content"><div class="modal-header"><button class="close close" data-dismiss="modal" type="button"><span aria-hidden="true">×</span><span class="sr-only">Close</span></button><h4 class="modal-title text-center"><strong>Log In</strong></h4></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><button class="btn btn-fb btn-lg btn-block btn-v-center-content" id="login-facebook-oauth-button"><svg style="float: left; width: 19px; line-height: 1em; margin-right: .3em;" aria-hidden="true" focusable="false" data-prefix="fab" data-icon="facebook-square" class="svg-inline--fa fa-facebook-square fa-w-14" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path fill="currentColor" d="M400 32H48A48 48 0 0 0 0 80v352a48 48 0 0 0 48 48h137.25V327.69h-63V256h63v-54.64c0-62.15 37-96.48 93.67-96.48 27.14 0 55.52 4.84 55.52 4.84v61h-31.27c-30.81 0-40.42 19.12-40.42 38.73V256h68.78l-11 71.69h-57.78V480H400a48 48 0 0 0 48-48V80a48 48 0 0 0-48-48z"></path></svg><small><strong>Log in</strong> with <strong>Facebook</strong></small></button><br /><button class="btn btn-google btn-lg btn-block btn-v-center-content" id="login-google-oauth-button"><svg style="float: left; width: 22px; line-height: 1em; margin-right: .3em;" aria-hidden="true" focusable="false" data-prefix="fab" data-icon="google-plus" class="svg-inline--fa fa-google-plus fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M256,8C119.1,8,8,119.1,8,256S119.1,504,256,504,504,392.9,504,256,392.9,8,256,8ZM185.3,380a124,124,0,0,1,0-248c31.3,0,60.1,11,83,32.3l-33.6,32.6c-13.2-12.9-31.3-19.1-49.4-19.1-42.9,0-77.2,35.5-77.2,78.1S142.3,334,185.3,334c32.6,0,64.9-19.1,70.1-53.3H185.3V238.1H302.2a109.2,109.2,0,0,1,1.9,20.7c0,70.8-47.5,121.2-118.8,121.2ZM415.5,273.8v35.5H380V273.8H344.5V238.3H380V202.8h35.5v35.5h35.2v35.5Z"></path></svg><small><strong>Log in</strong> with <strong>Google</strong></small></button><br /><style type="text/css">.sign-in-with-apple-button { width: 100%; height: 52px; border-radius: 3px; border: 1px solid black; cursor: pointer; } .sign-in-with-apple-button > div { margin: 0 auto; / This centers the Apple-rendered button horizontally }</style><script src="https://appleid.cdn-apple.com/appleauth/static/jsapi/appleid/1/en_US/appleid.auth.js" type="text/javascript"></script><div class="sign-in-with-apple-button" data-border="false" data-color="white" id="appleid-signin"><span   ="Sign Up with Apple" class="u-fs11"></span></div><script>AppleID.auth.init({ clientId: 'edu.academia.applesignon', scope: 'name email', redirectURI: 'https://www.academia.edu/sessions', state: "bad93a8098a7ba27e4342099645518fda7ed2030c2ca64c59a59e3414886b6aa", });</script><script>// Hacky way of checking if on fast loswp if (window.loswp == null) { (function() { const Google = window?.Aedu?.Auth?.OauthButton?.Login?.Google; const Facebook = window?.Aedu?.Auth?.OauthButton?.Login?.Facebook; if (Google) { new Google({ el: '#login-google-oauth-button', rememberMeCheckboxId: 'remember_me', track: null }); } if (Facebook) { new Facebook({ el: '#login-facebook-oauth-button', rememberMeCheckboxId: 'remember_me', track: null }); } })(); }</script></div></div></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><div class="hr-heading login-hr-heading"><span class="hr-heading-text">or</span></div></div></div></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><form class="js-login-form" action="https://www.academia.edu/sessions" accept-charset="UTF-8" method="post"><input type="hidden" name="authenticity_token" value="3Yr9L-KVgLe634IpqNbUbNcPJ4Hsp7Icp3yEw8bDHqszKZ_gqdfA0rkTT5k6mzShROBxVb_gf582HbUUHZ7fKw" autocomplete="off" /><div class="form-group"><label class="control-label" for="login-modal-email-input" style="font-size: 14px;">Email</label><input class="form-control" id="login-modal-email-input" name="login" type="email" /></div><div class="form-group"><label class="control-label" for="login-modal-password-input" style="font-size: 14px;">Password</label><input class="form-control" id="login-modal-password-input" name="password" type="password" /></div><input type="hidden" name="post_login_redirect_url" id="post_login_redirect_url" value="https://www.academia.edu/Documents/in/Peroxidase" autocomplete="off" /><div class="checkbox"><label><input type="checkbox" name="remember_me" id="remember_me" value="1" checked="checked" /><small style="font-size: 12px; margin-top: 2px; display: inline-block;">Remember me on this computer</small></label></div><br><input type="submit" name="commit" value="Log In" class="btn btn-primary btn-block btn-lg js-login-submit" data-disable-with="Log In" /></br></form><script>typeof window?.Aedu?.recaptchaManagedForm === 'function' && window.Aedu.recaptchaManagedForm( document.querySelector('.js-login-form'), document.querySelector('.js-login-submit') );</script><small style="font-size: 12px;"><br />or <a data-target="#login-modal-reset-password-container" data-toggle="collapse" href="javascript:void(0)">reset password</a></small><div class="collapse" id="login-modal-reset-password-container"><br /><div class="well margin-0x"><form class="js-password-reset-form" action="https://www.academia.edu/reset_password" accept-charset="UTF-8" method="post"><input type="hidden" name="authenticity_token" value="wmT1MZueXv1SQa-IvobA-xBzxIUGnpRbUSnsjA-RssMsx5f-0NwemFGNYjgsyyA2g5ySUVXZWdjASN1b1MxzQw" autocomplete="off" /><p>Enter the email address you signed up with and we'll email you a reset link.</p><div class="form-group"><input class="form-control" name="email" type="email" /></div><script src="https://recaptcha.net/recaptcha/api.js" async defer></script> <script> var invisibleRecaptchaSubmit = function () { var closestForm = function (ele) { var curEle = ele.parentNode; while (curEle.nodeName !== 'FORM' && curEle.nodeName !== 'BODY'){ curEle = curEle.parentNode; } return curEle.nodeName === 'FORM' ? curEle : null }; var eles = document.getElementsByClassName('g-recaptcha'); if (eles.length > 0) { var form = closestForm(eles[0]); if (form) { form.submit(); } } }; </script> <input type="submit" data-sitekey="6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj" data-callback="invisibleRecaptchaSubmit" class="g-recaptcha btn btn-primary btn-block" value="Email me a link" value=""/> </form></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/collapse-45805421cf446ca5adf7aaa1935b08a3a8d1d9a6cc5d91a62a2a3a00b20b3e6a.js"], function() { // from javascript_helper.rb $("#login-modal-reset-password-container").on("shown.bs.collapse", function() { $(this).find("input[type=email]").focus(); }); }); </script> </div></div></div><div class="modal-footer"><div class="text-center"><small style="font-size: 12px;">Need an account? <a rel="nofollow" href="https://www.academia.edu/signup">Click here to sign up</a></small></div></div></div></div></div></div><script>// If we are on subdomain or non-bootstrapped page, redirect to login page instead of showing modal (function(){ if (typeof $ === 'undefined') return; var host = window.location.hostname; if ((host === $domain || host === "www."+$domain) && (typeof $().modal === 'function')) { $("#nav_log_in").click(function(e) { // Don't follow the link and open the modal e.preventDefault(); $("#login-modal").on('shown.bs.modal', function() { $(this).find("#login-modal-email-input").focus() }).modal('show'); }); } })()</script> <div class="bootstrap" id="footer"><div class="footer-content clearfix text-center padding-top-7x" style="width:100%;"><ul class="footer-links-secondary footer-links-wide list-inline margin-bottom-1x"><li><a href="https://www.academia.edu/about">About</a></li><li><a href="https://www.academia.edu/press">Press</a></li><li><a href="https://www.academia.edu/documents">Papers</a></li><li><a href="https://www.academia.edu/topics">Topics</a></li><li><a href="https://www.academia.edu/journals">Academia.edu Journals</a></li><li><a rel="nofollow" href="https://www.academia.edu/hiring"><svg style="width: 13px; height: 13px;" aria-hidden="true" focusable="false" data-prefix="fas" data-icon="briefcase" class="svg-inline--fa fa-briefcase fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M320 336c0 8.84-7.16 16-16 16h-96c-8.84 0-16-7.16-16-16v-48H0v144c0 25.6 22.4 48 48 48h416c25.6 0 48-22.4 48-48V288H320v48zm144-208h-80V80c0-25.6-22.4-48-48-48H176c-25.6 0-48 22.4-48 48v48H48c-25.6 0-48 22.4-48 48v80h512v-80c0-25.6-22.4-48-48-48zm-144 0H192V96h128v32z"></path></svg> <strong>We're Hiring!</strong></a></li><li><a rel="nofollow" href="https://support.academia.edu/hc/en-us"><svg style="width: 12px; height: 12px;" aria-hidden="true" focusable="false" data-prefix="fas" data-icon="question-circle" class="svg-inline--fa fa-question-circle fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M504 256c0 136.997-111.043 248-248 248S8 392.997 8 256C8 119.083 119.043 8 256 8s248 111.083 248 248zM262.655 90c-54.497 0-89.255 22.957-116.549 63.758-3.536 5.286-2.353 12.415 2.715 16.258l34.699 26.31c5.205 3.947 12.621 3.008 16.665-2.122 17.864-22.658 30.113-35.797 57.303-35.797 20.429 0 45.698 13.148 45.698 32.958 0 14.976-12.363 22.667-32.534 33.976C247.128 238.528 216 254.941 216 296v4c0 6.627 5.373 12 12 12h56c6.627 0 12-5.373 12-12v-1.333c0-28.462 83.186-29.647 83.186-106.667 0-58.002-60.165-102-116.531-102zM256 338c-25.365 0-46 20.635-46 46 0 25.364 20.635 46 46 46s46-20.636 46-46c0-25.365-20.635-46-46-46z"></path></svg> <strong>Help Center</strong></a></li></ul><ul class="footer-links-tertiary list-inline margin-bottom-1x"><li class="small">Find new research papers in:</li><li class="small"><a href="https://www.academia.edu/Documents/in/Physics">Physics</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Chemistry">Chemistry</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Biology">Biology</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Health_Sciences">Health Sciences</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Ecology">Ecology</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Earth_Sciences">Earth Sciences</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Cognitive_Science">Cognitive Science</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Mathematics">Mathematics</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Computer_Science">Computer Science</a></li></ul></div></div><div class="DesignSystem" id="credit" style="width:100%;"><ul class="u-pl0x footer-links-legal list-inline"><li><a rel="nofollow" href="https://www.academia.edu/terms">Terms</a></li><li><a rel="nofollow" href="https://www.academia.edu/privacy">Privacy</a></li><li><a rel="nofollow" href="https://www.academia.edu/copyright">Copyright</a></li><li>Academia ©2025</li></ul></div><script> //<![CDATA[ window.detect_gmtoffset = true; window.Academia && window.Academia.set_gmtoffset && Academia.set_gmtoffset('/gmtoffset'); //]]> </script> <div id='overlay_background'></div> <div id='bootstrap-modal-container' class='bootstrap'></div> <div id='ds-modal-container' class='bootstrap DesignSystem'></div> <div id='full-screen-modal'></div> </div> </body> </html>