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Phase Diagrams and Liquid-Liquid Extraction in Aqueous Biphasic Systems Formed by Polyethylene Glycol and Potassium Sodium Tartrate at 303.15 K
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two-phase systems (ATPSs) constitutes a powerful tool for purifying bio-materials, such as cells, organelles, proteins, among others. In this work, the extraction of the bovine serum albumin (BSA) has been studied in systems formed by polyethylene glycol (PEG) (1500, 4000, and 6000 g.mol⁻¹) + potassium sodium tartrate + water at 303.15°K. Phase diagrams were obtained by turbidimetry and Merchuk’s method (1998). The experimental tie-lines were described using the Othmer-Tobias and Bancroft correlations. ATPSs were correlated with the nonrandom two-liquid (NRTL) model. The results were considered excellent according to global root-mean-square deviations found which were between 0,72 and 1,13%. The concentrations of the proteins in each phase were determined by spectrophotometry at 280 nm, finding partition efficiencies greater than 71%. <iframe src="https://publications.waset.org/abstracts/103304.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aqueous%20two%20phases%20systems" title="aqueous two phases systems">aqueous two phases systems</a>, <a href="https://publications.waset.org/abstracts/search?q=bovine%20serum%20albumin" title=" bovine serum albumin "> bovine serum albumin </a>, <a href="https://publications.waset.org/abstracts/search?q=liquid-liquid%20extraction" title=" liquid-liquid extraction"> liquid-liquid extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=polyethylene%20glycol" title=" polyethylene glycol"> polyethylene glycol</a> </p> <a href="https://publications.waset.org/abstracts/103304/phase-diagrams-and-liquid-liquid-extraction-in-aqueous-biphasic-systems-formed-by-polyethylene-glycol-and-potassium-sodium-tartrate-at-30315-k" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/103304.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">157</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates 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