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class="bread-arrow"></span> <span class="breadcrumb_last" aria-current="page">Single-cell Sequencing Applications in Cancer</span></span></div></div> <div class="secondary-hero new-library"> <div class="_1300-container inner-content sec-content"> <div class="library-col"> <div class="library-icon-text"><img src="/wp-content/themes/missionbio-v2/images/Icon-Learning-02.svg" loading="lazy" alt="" class="image-3"></div> </div> <div class="library-col"> <div>Mission Bio is committed to helping you learn about single-cell DNA sequencing and multi-omics and how these exciting technologies are advancing medical science. Enjoy exploring our education hub!</div> </div> </div> </div> <div class="learning-content"> <div class="_1300-container lc"> <div class="lc-columns"> <div class="lc-navigation"> <a href="https://missionbio.com/resources/learning-center/clonal-evolution-in-cancer/" class="lc-chapter expanding w-inline-block"> <div>Clonal Evolution in Cancer</div> </a> <div class="lc-sub-chapters"> <a href="https://missionbio.com/resources/learning-center/clonal-evolution-in-cancer/#clonal-evolution-drives-tumor-heterogeneity" class="lc-sub-link">Clonal Evolution Drives Tumor Heterogeneity</a> <a href="https://missionbio.com/resources/learning-center/clonal-evolution-in-cancer/#tumors-have-diverse-mutation-types" class="lc-sub-link">Tumors Have Diverse Mutation Types</a> <a href="https://missionbio.com/resources/learning-center/clonal-evolution-in-cancer/#clonal-evolution-in-cancer-has-clinical-implications" class="lc-sub-link">Clonal Evolution in Cancer Has Clinical Implications</a> </div> <a href="https://missionbio.com/resources/learning-center/dissecting-cancer-single-cell-dna-sequencing-multi-omics/" class="lc-chapter expanding w-inline-block"> <div>Dissecting Cancer with Single-cell DNA Sequencing & Multi-omics</div> </a> <div class="lc-sub-chapters"> <a href="https://missionbio.com/resources/learning-center/dissecting-cancer-single-cell-dna-sequencing-multi-omics/#single-cell-dna-sequencing-uncovers-genetic-variation-in-tumors" class="lc-sub-link">Single-cell DNA Sequencing Uncovers Genetic Variation in Tumors</a> <a href="https://missionbio.com/resources/learning-center/dissecting-cancer-single-cell-dna-sequencing-multi-omics/#the-power-of-single-cell-multi-omics" class="lc-sub-link">The Power of Single-Cell Multi-omics</a> </div> <a href="https://missionbio.com/resources/learning-center/single-cell-sequencing-applications-in-cancer/" class="lc-chapter expanding w-inline-block"> <div>Single-cell Sequencing Applications in Cancer</div> </a> <div class="lc-sub-chapters"> <a href="https://missionbio.com/resources/learning-center/single-cell-sequencing-applications-in-cancer/#tumor-evolution" class="lc-sub-link">Tumor Evolution</a> <a href="https://missionbio.com/resources/learning-center/single-cell-sequencing-applications-in-cancer/#therapeutic-resistance" class="lc-sub-link">Therapeutic Resistance</a> <a href="https://missionbio.com/resources/learning-center/single-cell-sequencing-applications-in-cancer/#minimal-residual-disease-mrd" class="lc-sub-link">Minimal Residual Disease (MRD)</a> <a href="https://missionbio.com/resources/learning-center/single-cell-sequencing-applications-in-cancer/#clonal-hematopoiesis-of-indeterminate-potential-chip" class="lc-sub-link">Clonal Hematopoiesis of Indeterminate Potential (CHIP)</a> </div> <a href="https://missionbio.com/resources/learning-center/single-cell-analysis-genome-engineering/" class="lc-chapter expanding w-inline-block"> <div>Single-cell Analysis of Genome Engineering</div> </a> <div class="lc-sub-chapters"> <a href="https://missionbio.com/resources/learning-center/single-cell-analysis-genome-engineering/#methods-of-genome-engineering" class="lc-sub-link">Methods of Genome Engineering</a> <a href="https://missionbio.com/resources/learning-center/single-cell-analysis-genome-engineering/#loss-of-function-screening" class="lc-sub-link">Loss-of-function Screening</a> <a href="https://missionbio.com/resources/learning-center/single-cell-analysis-genome-engineering/#disease-modeling" class="lc-sub-link">Disease Modeling</a> <a href="https://missionbio.com/resources/learning-center/single-cell-analysis-genome-engineering/#cell-and-gene-therapies" class="lc-sub-link">Cell and Gene Therapies</a> </div> <a href="https://missionbio.com/resources/learning-center/references/" class="lc-chapter w-inline-block"> <div>References</div> </a> <a href="https://missionbio.com/resources/ebooks/single-cell-sequencing-101/" class="lc-side-cta w-inline-block"> <div class="side-cta-title"><img src="/wp-content/themes/missionbio-v2/images/Icon-Guide-01.svg" loading="lazy" alt="" class="side-cta-icon"> <div>Download our 101 ebook</div> </div> <div>Learn the basics of single-cell sequencing.</div> </a> <a href="https://missionbio.com/company/contact/" class="lc-side-cta bottom w-inline-block"> <div class="side-cta-title"><img src="/wp-content/themes/missionbio-v2/images/Icon-Call-01.svg" loading="lazy" alt="" class="side-cta-icon"> <div>Speak to an expert</div> </div> <div>See how single-cell analysis can advance your research.</div> </a> </div> <div class="lc-content"> <h5>CANCER & SINGLE-CELL ANALYSIS</h5><a class="anchor" id="single-cell-sequencing-applications-in-cancer"></a><h1>Single-cell Sequencing Applications in Cancer</h1><p>Translational research is the bridge that links fundamental discovery to clinical application. Profiling cancer leads to many advancements, including biomarker discovery and characterizing disease processes. This information lends insight into how we can better detect and surveil disease, segment patients into clinical trials, and combat resistance to therapies. Here, we outline four salient single-cell sequencing applications in cancer research: tumor evolution, therapeutic resistance, minimal residual disease (MRD), and clonal hematopoiesis of indeterminate potential (CHIP).</p> <a class="anchor" id="tumor-evolution"></a><h2>Tumor Evolution</h2><p>One growing single-cell sequencing application is the reconstruction of tumor evolutionary histories. Because single-cell analysis assigns mutations to different clones, phylogenetic trees can be reconstructed based on patterns of co-mutation.</p> <p>Reassembling clonal histories not only reveals the overall pattern of evolution (linear vs. branched) but also enables researchers to understand other important aspects of disease progression. For instance, disease-initiating mutations, as well as patterns of mutual exclusivity and co-occurrence, are identified. Assessing cooperative mutations that give cells a fitness advantage is particularly illuminating. For instance, in the AML phylogeny depicted in Figure 11, the co-mutations, <em>DNMT3A</em> and <em>NPM1</em>, enabled a clone (dark blue circle) to expand and compose about 30% of the tumor.</p> <p>Phylogenetic studies that leverage single-cell analysis are contributing valuable insights into cancer. For instance, Miles et al. (2020) revealed the chronological order of mutational acquisition as well as cooperative mutations that drove clonal expansion in AML. This study also found that evolutionary patterns differed across stages of disease progression.<sup>5</sup> In another AML study, Morita et al. (2020) discovered linear and branched patterns of evolution, with some subclones independently acquiring mutations in the same genes (convergent evolution). Proteogenomic profiling in both of these studies enabled the simultaneous characterization of mutational profile and cell type/state in an evolutionary context.<sup>4</sup></p> <p>These studies are shedding new light on the behavior of cancer. Not only do these endeavors contribute foundational knowledge to malignant transformation, but there is mounting evidence they have prognostic value. For instance, higher intratumoral heterogeneity is associated with the development of drug resistance and poorer prognosis in AML.<sup>9,10</sup> In the heme field alone a number of indications are actively being investigated, including CH, AML, MPN, MDS, multiple myeloma, CLL, and ALL.<sup>4,5, 11-13</sup> As tumors continue to be profiled across patients, general patterns of evolution will likely become apparent.</p> <div id="attachment_8386" class="wp-caption aligncenter"><img aria-describedby="caption-attachment-8386" fetchpriority="high" decoding="async" class="size-full wp-image-8386" src="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12.png" alt="Figure 11. Evolutionary analysis reveals clonal dynamics." width="2227" height="1662" srcset="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12.png 2227w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-300x224.png 300w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-1024x764.png 1024w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-768x573.png 768w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-1536x1146.png 1536w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-2048x1528.png 2048w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-1800x1343.png 1800w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-1440x1075.png 1440w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-1280x955.png 1280w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-480x358.png 480w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-12-240x179.png 240w" sizes="(max-width: 2227px) 100vw, 2227px" /><p id="caption-attachment-8386" class="wp-caption-text"><strong>Figure 11. Evolutionary analysis reveals clonal dynamics.</strong> In this AML phylogenetic tree, it is evident that early mutations (DNMT3A and NPM1) are clonal and subsequent mutations (FLT3 and NRAS) are subclonal. Clonal expansion events are also evident (yellow circle: DNMT3A-NPM1-FLT3 mutant; dark blue circle: DNMT3A-NPM1 mutant).</p></div> <a class="anchor" id="therapeutic-resistance"></a><h2>Therapeutic Resistance</h2><p>Of the many single-cell sequencing applications in oncology, the use of single-cell data to identify mechanisms of therapeutic resistance holds a lot of promise in personalized medicine. Although anti-cancer therapies often succeed in bringing patients into <strong>complete remission (CR)</strong>, tumors often relapse at a later time point. The failure to eliminate cancer entirely is often due to the heterogeneity and dynamism of the disease.</p> <p>Tumor cells can evade treatment through <strong>primary resistance</strong>, in which pre-existing mutations offer protective properties. Alternatively, <strong>secondary resistance</strong> may emerge through mutations acquired during the course of treatment. Because most tumor cells succumb to therapy, cells that survive have little competition for resources. Even small initial quantities of resistant cells can rapidly expand and drive relapse. The ability for tumors to adapt in this fashion lies at the root of therapeutic failure.</p> <p>Though cancer’s uncanny ability to evade treatments remains a significant challenge, understanding this process may enable us to beat cancer at its own game. Single-cell DNA sequencing offers considerable value over traditional bulk analysis. Because bulk is unable to unambiguously delineate mutational co-occurrence across different clones, it is not possible to determine whether <em>all </em>clones will be targeted by a given therapy. Single-cell DNA sequencing can detect high-risk clones early on so that treatment plans can be adjusted to reduce the likelihood of relapse.</p> <p>To illustrate the value of single-cell analysis, consider the example of a longitudinal study of a relapsed AML patient who received the targeted <em>FLT3</em> inhibitor therapy, gilteritinib (Fig 12). At the time the patient was treated, peripheral blood was tested at three timepoints (diagnosis, during therapy, and at relapse) using bulk NGS. Because bulk analysis cannot resolve clonal structure, there was no way to determine the clones present at each time point. When the blood samples were analyzed with single-cell DNA sequencing in a retrospective study, the tumor architecture was revealed.</p> <p>This lack of clonal resolution in the initial study meant that clones that led to relapse were missed. For instance, at the diagnosis timepoint, the light blue clone was effectively targeted by gilteritinib while the dark blue clone was not (due to lacking the <em>FLT3</em> target). This latter clone expanded post-therapy and contributed to relapse. Similarly, a novel clone (red) that emerged during treatment had acquired resistance to gilteritinib via an <em>NRAS</em> mutation. This clone was too rare to be detected by bulk NGS during treatment (comprising only 0.1% of the tumor). Hence, it was free to expand unabated and contributed to relapse.</p> <div id="attachment_8387" class="wp-caption aligncenter"><img aria-describedby="caption-attachment-8387" decoding="async" class="size-full wp-image-8387" src="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13.png" alt="Figure 12. Clonal structure underlies resistance mechanisms." width="2237" height="1770" srcset="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13.png 2237w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-300x237.png 300w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-1024x810.png 1024w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-768x608.png 768w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-1536x1215.png 1536w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-2048x1620.png 2048w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-1800x1424.png 1800w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-1440x1139.png 1440w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-1280x1013.png 1280w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-480x380.png 480w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-13-240x190.png 240w" sizes="(max-width: 2237px) 100vw, 2237px" /><p id="caption-attachment-8387" class="wp-caption-text"><strong>Figure 12. Clonal structure underlies resistance mechanisms.</strong> A retrospective study that used single-cell DNA sequencing to analyze blood at three timepoints (diagnosis, during treatment, relapse). Single-cell analysis detected clones (dark blue and red) that ultimately drove relapse in the patient.</p></div> <p>Using single-cell data to inform personalized treatment strategies holds promise for reducing rates of relapse. By resolving the clonal structure of a tumor prior to treatment, combinational therapies can be developed to ensure all clones are targeted. Surveillance during treatment can also ensure that any new therapy-resistant clones are identified. An increasing number of studies are using single-cell DNA sequencing to uncover mechanisms of resistance in clinical trials, further underscoring the potential of this technology in a therapeutic setting.<sup>4,5,8,14</sup></p> <a class="anchor" id="minimal-residual-disease-mrd"></a><h2>Minimal Residual Disease (MRD)</h2><p>Another impactful single-cell sequencing application is the detection of small numbers of malignant cells — called <strong>minimal residual disease (MRD)</strong> — present in the body. Despite advances in anti-cancer therapeutics, it is not uncommon for resistant cells to escape therapy and drive relapse. Even for patients that have achieved complete remission, small quantities of remaining pathogenic cells often remain. For myeloid malignancies, MRD is a powerful predictor of treatment outcomes. Data from clinical trials have revealed that the presence of MRD is indicative of both faster relapse and lower overall survival (Fig 13).<sup>15</sup> MRD is becoming established as a surrogate marker for relapse-free survival and could one day be used as a regulatory endpoint for drug approval.<sup>16</sup></p> <p>Given its clinical implications, MRD detection assays are commonly performed following induction chemotherapy. If malignant clones are detected and characterized, early action can be taken to intervene. Conventionally, MRD is identified by either genotype or immunophenotype. Traditional genotypic methods like PCR, qPCR, or bulk NGS detect mutation frequencies but do not assign aberrations to distinct clones. Without this clonal structure, the identification of malignant cells is limited.</p> <p>MRD may also be detected through immunophenotyping (typically through multi-parameter flow cytometry). In AML, for instance, MRD is identified by flow-sorting cells based on the abnormal pattern of markers expressed by blast cells. However, the sensitivity of this assay is limited, as common markers are expressed by both healthy and leukemic cells.</p> <p>Single-cell multi-omics (DNA + protein) analysis combines the strengths of both assays into a single workflow. The use of multiple analytes to detect aberrant cells enhances the detection of residual disease.<sup>17,18</sup> For example, Dillon et al. (2020) reported an AML case in which leukemic cell-surface markers were present on only half of cells with malignant genotypes.<sup>18</sup> Here the use of DNA + protein multi-omics enabled MRD to be characterized to a fuller extent than either analyte could have alone. In the clinic, this highly-specific information can be used to guide personalized treatment regimens.</p> <div id="attachment_8388" class="wp-caption aligncenter"><img aria-describedby="caption-attachment-8388" decoding="async" class="size-full wp-image-8388" src="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14.png" alt="Figure 13. MRD status correlates with clinical outcomes." width="2872" height="947" srcset="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14.png 2872w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-300x99.png 300w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-1024x338.png 1024w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-768x253.png 768w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-1536x506.png 1536w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-2048x675.png 2048w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-1800x594.png 1800w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-1440x475.png 1440w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-1280x422.png 1280w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-480x158.png 480w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-14-240x79.png 240w" sizes="(max-width: 2872px) 100vw, 2872px" /><p id="caption-attachment-8388" class="wp-caption-text"><strong>Figure 13. MRD status correlates with clinical outcomes.</strong> In these hypothetical Kaplan-Meier curves, patients with MRD tend to have a higher incidence of relapse and lower overall survival.</p></div> <a class="anchor" id="clonal-hematopoiesis-of-indeterminate-potential-chip"></a><h2>Clonal Hematopoiesis of Indeterminate Potential (CHIP)</h2><p>Single-cell analysis can aid in the identification of MRD, but distinguishing diseased cells from benign ones is not always clear-cut. For blood cancers, a relatively recent single-cell sequencing application is the ability to distinguish malignant cells from non-malignant ones that share some of the same mutations.</p> <p>Blood cancers like AML begin with hematopoietic stem cells (HSCs) — cells that reside in the bone marrow and give rise to all descendant blood cells. These cells rarely divide and thus have relatively stable genomes. However, as humans age they gradually acquire somatic mutations. Mutations in a particular set of epigenetic regulators (commonly <em>DNMT3A, TET2,</em> and<em> ASXL1</em>) give HSCs a competitive edge, enabling them to clonally expand in a process called <strong>clonal hematopoiesis of indeterminate potential (CHIP)</strong>. Although these mutations lead to clonal subpopulations, the cells are not yet malignant and do not cause symptoms of disease. However, they are predisposed to acquiring additional mutations that lead to full leukemic transformation. Thus, people with CHIP mutations are considered to have the <em>potential</em> to develop hematologic malignancies like myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML).</p> <p>CHIP is an age-driven process, occurring in about ~ 20% of the US population. Somatic mutations in epigenetic genes (occurring at ≥ 0.02 VAF) appear in middle age and increase over time (Fig 14).<sup>19</sup> In addition to a higher risk of developing a hematologic malignancy, the presence of CHIP has also been correlated with a higher risk of developing cardiovascular disease and reduced lifespan.</p> <div id="attachment_8389" class="wp-caption aligncenter"><img aria-describedby="caption-attachment-8389" decoding="async" class="wp-image-8389" src="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15.png" alt="Figure 14. CHIP progresses with age." width="720" height="428" srcset="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15.png 1782w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15-300x178.png 300w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15-1024x609.png 1024w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15-768x456.png 768w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15-1536x913.png 1536w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15-1440x856.png 1440w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15-1280x761.png 1280w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15-480x285.png 480w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-15-240x143.png 240w" sizes="(max-width: 720px) 100vw, 720px" /><p id="caption-attachment-8389" class="wp-caption-text"><strong>Figure 14. CHIP progresses with age.</strong> CHIP is a non-malignant condition characterized by mutation and clonal expansion of blood cells, beginning at middle age. CHIP is associated with a higher risk of developing hematological malignancies and cardiovascular disease, as well as a reduced lifespan.</p></div> <p>The etiology of CHIP is not well understood. For clinicians, one area of particular interest is the ability to differentiate CHIP (premalignant) and oncogenic clones. Because CHIP cells have mutations that are also found in leukemic cells, it is often difficult to distinguish the two. However, the clonal structure of these mutations can be used to verify whether cells are pathogenic or not.</p> <p>The clonal structure of residual disease can be evaluated using single-cell DNA sequencing.<sup>17</sup> As an illustrative example, Figure 15 shows a cell population with several genetic aberrations at low frequencies. While the substructure of the tumor cannot be determined from the VAFs alone (an output of bulk NGS), it can be resolved with single-cell DNA sequencing. Here, the two leukemic clones have a gene fusion in chromosome 16 (a defining feature of AML). The CHIP clones have single mutations in either <em>TET2</em> or <em>ASXL1</em> that do not co-occur with the chromosome 16 fusion (indicating they are not leukemic).</p> <div id="attachment_8390" class="wp-caption aligncenter"><img aria-describedby="caption-attachment-8390" decoding="async" class="wp-image-8390" src="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16.png" alt="Figure 15. Single-cell analysis distinguishes CHIP clones." width="720" height="534" srcset="https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16.png 1901w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-300x222.png 300w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-1024x759.png 1024w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-768x569.png 768w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-1536x1138.png 1536w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-1800x1334.png 1800w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-1440x1067.png 1440w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-1280x949.png 1280w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-480x356.png 480w, https://missionbio.com/wp-content/uploads/2021/08/mission_bio_graphics_2021-16-240x178.png 240w" sizes="(max-width: 720px) 100vw, 720px" /><p id="caption-attachment-8390" class="wp-caption-text"><strong>Figure 15. Single-cell analysis distinguishes CHIP clones.</strong> Pre-malignant (CHIP) and malignant clones cannot be distinguished from VAFs alone. By resolving the mutational structure across clones, single-cell DNA sequencing enables the identification of CHIP and leukemic clones.</p></div> <p>Single cell DNA+ protein multi-omics can also be leveraged to distinguish MRD from CHIP clones. By layering genotypic and immunophenotypic information, malignant and non-malignant clones can be identified with greater confidence. Indeed, Dillon et al. (2020) used single-cell proteogenomic profiling to assess residual disease in relapsed AML patients.</p> <p>Whereas traditional methods of MRD identification — genetic sequencing and flow cytometry — are often discordant, this study was able to overcome this limitation by combining genotypic and immunophenotypic profiling. Using single-cell multi-omics, the authors were able to confidently resolve the clonal architecture of three patients and distinguish CHIP and AML clones.<sup>18</sup></p> <div class="lc-cta"><div class="lc-cta-content"><div class="lc-cta-image" style="background-image: url('https://missionbio.com/wp-content/themes/missionbio-v2/images-2/LC-CTA-Img.jpg');"></div><div class="lc-cta-text"><div class="lc-cta-title">Interested in Single Cell for Pharma Applications?</div><div>Leverage single-cell technology to identify mechanisms of resistance and accelerate your drug development pipelines.</div><a class="button lc-button w-button" href="https://missionbio.com/products/pharma-assay-development/">Learn More</a></div></div></div> <div class="lc-vocabulary"><h3 class="lc-box-titile">Vocabulary</h3></p> <p><strong>Complete remission (CR):</strong> no cancer is detected by standard lab tests and imaging.</p> <p><strong>Primary resistance:</strong> pre-existing mutations confer resistance to a therapeutic.</p> <p><strong>Secondary resistance:</strong> mutations that emerge during treatment confer resistance to a therapeutic.</p> <p><strong>Minimal residual disease (MRD):</strong> small quantities of cancer cells that remain in the body following cancer treatment. Also called: measurable residual disease.</p> <p><strong>Clonal hematopoiesis of indeterminate potential (CHIP): </strong>the process of mutational acquisition and subsequent clonal expansion of hematopoietic stem cells (HSCs), resulting in non-malignant clones. Also called: age-related clonal hematopoiesis</p> <p></div> </div> </div> </div> </div> <script type="text/javascript" src="https://missionbio.com/wp-content/themes/missionbio-v2/js/jquery-3.4.1.min.js?ver=1590639462" id="jquery-js"></script> <script type="text/javascript" src="https://missionbio.com/wp-content/themes/missionbio-v2/js/swiper-bundle.min.js?ver=1.0" id="swiper-script-js"></script> <script type="text/javascript" src="https://missionbio.com/wp-content/themes/missionbio-v2/js/slick.min.js?ver=1.0" id="slick-script-js"></script> <script type="text/javascript" src="https://missionbio.com/wp-content/themes/missionbio-v2/js/mission-bio.js?ver=1666878465" id="mb-scripts-js"></script> <script type="text/javascript" src="https://missionbio.com/wp-content/themes/missionbio-v2/script.js?ver=1727345272" id="mb-main-js"></script> <script type="text/javascript" src="https://missionbio.com/wp-content/plugins/pardot/js/asyncdc.min.js?ver=6.7" id="pddc-js"></script> <div class="g-footer"> <div class="_1300-container inner-content"> <div class="g-footer-grid"> <div class="g-footer-col-logo"> <a href="/" class="g-footer-logo w-inline-block"> <img src="/wp-content/themes/missionbio-v2/images/MB-Logo-Stacked-White.svg" alt=""> </a> <div class="g-footer-social-grid"> <a target="_blank" href="https://twitter.com/missionbio?lang=en" class="g-footer-social-link tw w-inline-block"></a> <a target="_blank" href="https://www.facebook.com/missionbio/" class="g-footer-social-link fb w-inline-block"></a> <a target="_blank" href="https://www.linkedin.com/company/mission-bio" class="g-footer-social-link ln w-inline-block"></a> <a target="_blank" href="https://www.youtube.com/channel/UC3xFKde4blclowu-FbYMFeA" class="g-footer-social-link yt w-inline-block"></a> </div> <div class="g-footer-title">HEADQUARTERS</div> <div class="address">400 E Jamie Court, Suite 100<br>South San Francisco, CA 94080</div> <a href="tel:+14158540058" class="g-footer-link mb">+1 (415) 854-0058</a> <a href="mailto:info@missionbio.com" class="g-footer-link">info@missionbio.com</a> <div class="f-copyright">©2024 Mission Bio. 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