Whole Plasmid Sequencing | Plasmid-EZ

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high-throughput sequencing of entire plasmids. Unlike conventional methods that only sequence selected regions, Plasmid-EZ verifies the integrity and fidelity of whole plasmids using long-read sequencing. This ensures the detection of unwanted changes, providing a complete plasmid map and preventing potential replication issues. </p> <p>Now connected to our expansive <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/dropboxrequest" target="_blank">dropbox</a> network, Plasmid-EZ offers unparalleled convenience for sample submission – easily order directly through our customer portal and deposit your samples at one of our convenient dropboxes. Upon receipt in our processing lab, you will receive results in as fast as one business day. </p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/NGS?lobId=3&serviceId=3200" target="_blank"><button>Order Now</button></a>  </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02VT2pB0" target="_blank"><img alt="" src="/-/media/Plasmid-EZ-EOY-2024-Right-Banner-Ad.ashx?la=en&hash=A2F7137DB328C7088BA841515119798D84ADAD59" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 99%; text-align: left; } .question ul { width: 90%; text-align: left; } .question button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; } .question button:hover { background: #6965a1; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is whole plasmid sequencing?</h2> <p>Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).</p> <p>Whole plasmid sequencing provides detailed insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies. By sequencing the whole plasmid, genomic data can be used to further the understanding of microbial diversity, evolution, and mechanisms underlying biological processes.</p> <p> </p> <h2>How is whole plasmid sequencing done?</h2> <p>Whole plasmid sequencing is carried out by isolating the plasmid DNA (pDNA), splicing the pDNA into smaller fragments, sequencing said fragments, then assembling the plasmid sequence data to reconstruct the full plasmid genome. Bioinformatics tools are then used to analyze the resulting sequencing data.</p> <p> </p> <h2>Direct Colony to Whole Plasmid Sequencing</h2> <p>Simplify your research workflow and enhance your productivity with our advanced whole plasmid sequencing service (Plasmid-EZ), <strong>now accepting bacterial samples as starting material</strong>. With our experts performing plasmid preps and sequencing, we’ve streamlined our process to efficiently deliver results in as fast as 2 business days. Experience the ease and excellence of our sequencing solutions today.</p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/direct-colony-to-plasmid-ez" target="_blank"><button>Learn More</button></a> <p> </p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .protocol { width: 1170px; display: inline-block; text-align: center; } .protocol img { width: 98%; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .protocol { width: auto; } } </style> <div class="protocol"> <h2>PLASMID CONSTRUCTION WORKFLOW WITH PLASMID-EZ</h2> <p> </p> <p><img alt="" src="/-/media/11035-D_Plasmid-Construction_Workflow-3x-3.ashx?w=1170px&la=en&hash=FFDCE65830370037B0CAC065D11A0D12FFCF6F02" width="1170px" /></p> </div> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .protocol { width: 1170px; display: inline-block; text-align: center; 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background-color: #826ec3;">Metric</td> <td style="text-align: center; background-color: #826ec3;">Sanger Sequencing</td> <td style="text-align: center; background-color: #826ec3;">Plasmid-EZ</td> <td style="text-align: center; background-color: #826ec3;">Primer Walking</td> <td style="text-align: center; background-color: #826ec3;">NGS</td> </tr> <tr class="panel-heading"> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>Starting Material</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;">Plasmid DNA</td> <td style="text-align: center; background-color: #d9d3ed; color:#000000;">Plasmid DNA or Bacteria*</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">Plasmid DNA</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">Plasmid DNA</td> </tr> <tr class="panel-heading"> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>Region Sequenced</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;">Targeted Regions</td> <td style="text-align: center; background-color: #d9d3ed; color:#000000;">Full-Length Plasmid</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">Long Targeted Regions</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">Full-Length Plasmid</td> </tr> <tr class="panel-heading"> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>Cost</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;">$</td> <td style="text-align: center; background-color: #d9d3ed; color:#000000;">$$</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">$$$</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">$$$</td> </tr> <tr class="panel-heading"> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>Read Quality</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;">+++</td> <td style="text-align: center; background-color: #d9d3ed; color:#000000;">++</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">+++</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">++++</td> </tr> <tr class="panel-heading"> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>Read Length</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;">++</td> <td style="text-align: center; background-color: #d9d3ed; color:#000000;">++++</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">++</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">+/++++</td> </tr> <tr class="panel-heading"> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>Automatic Assembly</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> <td style="text-align: center; background-color: #d9d3ed; color:#000000;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> </tr> <tr class="panel-heading"> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>Gene Annotations</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> <td style="text-align: center; background-color: #d9d3ed; color:#000000;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> </tr> <tr class="panel-heading"> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>Other Requirements</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;">Must provide primer</td> <td style="text-align: center; background-color: #d9d3ed; color:#000000;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> <td style="text-align: center; background-color: #ffffff; color:#000000;">Must provide reference</td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> </tr> </tbody> </table> <p style="text-align: left;"><small>*We accept bacterial samples as colonies, liquid culture and glycerol stock</small></p> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>Deliverables</h2> <p> • FASTQ files<br /> • Read-length and quality report<br /> • Consensus plasmid assembly<br /> • Plasmid annotation files</p> <p> </p> <p>Confirm your plasmid sequences with Azenta, a worldwide leader in sequencing since 1999</p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/RegisterAccount/Login" target="_blank"><button style="background: #826ec3; color: #fff; font-size: 15px; border-radius: 4px; border: none; padding: 10px 20px;">Order Now</button></a> <p> </p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .workflow-container { padding-top: 30px; background: #fffffff; } .workflow { text-align: center; width: 1170px; display: inline-block } .workflow-step { vertical-align: top; width: 30%; padding: 10px; margin-bottom: 20px; display: inline-block; } .workflow-step img { width: 150px; margin: 0 auto; margin-bottom: 20px; } .workflow-step h2 { width: 80%; text-align: center; } .workflow-step h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .workflow-step p { width: 80%; margin: 0 auto; text-align: center; } .workflow-icon { text-align: center; } @media only screen and (max-width: 1170px) { .workflow-container { width: auto; } .workflow { text-align: center; width: auto; } .workflow-step { width: 100%; } } </style> <div class="workflow-container"> <div class="workflow"> <h2 style="text-align: center; margin-bottom: 30px;">Features & Benefits</h2>   <div class="workflow-step"> <div class="workflow-icon"> <img alt="Rapid Turnaround Time Icon" src="/-/media/15016DPlasmidEZFBTATTIme.ashx?la=en&hash=141984B1D792678EC3ACCF307AFB9FC4045E0868" /> </div> <h3><strong>Rapid Turnaround Times</strong></h3> <p>Same-day results in designated areas or in as fast as 1 business day</p> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="Submit Samples Icon" src="/-/media/15016DPlasmidEZFBSubmitSamples.ashx?la=en&hash=979195DDAA141ABE8E1114220AB920395443415D" /> </div> <h3><strong>Easily Submit Samples</strong></h3> <p>At any one of our convenient dropbox locations</p> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="Data Report Icon" src="/-/media/15016DPlasmidEZFBDataReport.ashx?la=en&hash=4C9BAE79CA142D7B598FBDC855DAC93809D59FC8" /> </div> <h3><strong>Interactive Data Report</strong></h3> <p>Provides an easy way to understand sequencing results</p> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> <p style="margin-top: 0pt; margin-bottom: 0pt; margin-left: 0in; text-align: left;"><span style="color: #000000;">High-throughput, plasmid-to-data solution for rapid and interactive construct validation</span></p> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> <p style="margin-top: 0pt; margin-bottom: 1.24pt; margin-left: 0in; text-align: left;"><span style="color: #000000;">Same price across all plasmids, regardless of size and complexity</span></p> </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> <p style="margin-top: 0pt; margin-bottom: 0pt; margin-left: 0in; text-align: left;"><span style="color: #000000;">Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates</span></p> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> <p style="margin-top: 0pt; margin-bottom: 0pt; margin-left: 0in; text-align: left;"><span style="color: #000000;">Flexible sample types accepted – plasmid DNA, bacterial colonies, liquid culture, and glycerol stock</span></p> </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; 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letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 15px; color: #ffffff; } #banner3-left img { width: auto; height: auto; } #banner3-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner3-left img { width: 60%; } #banner3-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner3"> <div id="banner3-left"> <img alt="" src="/-/media/15019-D_Boost-Plasmid-Prep_WebBanFull-Lrg_Thumbnail.ashx?la=en&hash=E5E2012D8959A6E170148EFB23E9A124285B1AFD" /> </div> <div id="banner3-right"> <h2>Boost Plasmid Yield<br /> and Purity</h2> <h3>Download our tech note for tips<br /> to enhance your results</h3> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02Y31w40" target="_blank"><button>DOWNLOAD</button></a> </div> </div> </div> <p> </p> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Gray margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h2>Technical Resources</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <a href='https://www.genewiz.com/en/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Prepare-Samples_210x110_purple.ashx?h=111&la=en&w=211&hash=8626211B8F77D0FB6757AF5D5E5A2960B52652D6" alt="Building" width="211" height="111" /> </div> <div class="row"> <h3>Sample Submission Guidelines</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='https://web.genewiz.com/hubfs/2023-04%20GEN%20NGS%20-%20Plasmid-EZ%20Launch/Quick%20Start%20Guide/15016-M&G-2%200623%20Plasmid-EZ%20Quick%20Start%20Guide.pdf' target='blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Single_Cell_icon_light_Purple.ashx?h=110&la=en&w=210&hash=3284394AA032C16207F15808EE49440DF386BC36" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Plasmid-EZ Quick Start Guide</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='https://web.genewiz.com/ngs-plasmid-ez-faq' target='blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/FAQ_210x110_purple.ashx?h=111&la=en&w=211&hash=3E72FE67CF61B960C891CE902CD430B183BB8BF7" alt="Building" width="211" height="111" /> </div> <div class="row"> <h3>Frequently Asked Questions</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; 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} } </style> <div style="width: 100%; background-color: #f0f0f0; padding: 0; margin: 0;margin-top: -20px;"> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/ONT-Blog.ashx?h=160&w=220&la=en&hash=AFF32CFD100B581BA8F5F09D2DF7011B7407F301" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Ensure Complete Plasmid Validation on Your First Attempt with Oxford Nanopore Sequencing</h3> <p>Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information. <br /> <a href="https://www.azenta.com/learning-center/blog/ensure-complete-plasmid-validation-your-first-attempt-oxford-nanopore-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Webinar--Sanger-ONT.ashx?h=160&w=220&la=en&hash=B46A4AA56DE351DD45E75213BD6FD1AA5AB8F799" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing</h3> <p>While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs. <br /> <a href="https://web.genewiz.com/workshop/base-pairs-to-breakthroughs" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Sanger-Trouble-shooting-blog.ashx?h=160&w=220&la=en&hash=24FECB44F887ED8D2D363153D5842A55828C120E" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Troubleshooting DNA Templates with Sanger Sequencing</h3> <p>Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.<br /> <a href="https://www.azenta.com/learning-center/blog/troubleshooting-dna-templates-sanger-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Optimizing-Plasmid-Prep-Tech-note.ashx?h=160&w=220&la=en&hash=70DB5E1BAC84C7138BCFF66873D37DF832B53635" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins</h3> <p>Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results. <br /> <a href="https://web.genewiz.com/technote/optimizingplasmidprep" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <p> </p> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="row"> <h2>NGS Platforms</h2> <p>Azenta Life Sciences is a service provider of cutting-edge NGS platforms. For information on our NGS platforms and recommended configurations of your projects, please visit the <a href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms">NGS Platforms</a> page. <em>Azenta does not guarantee data output or quality for sequencing-only projects.</em> </p> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><img alt="Illumina" src="/-/media/hires-illumina-logo-rgb_new.ashx?w=200&la=en&hash=2DE7778E5877DA9F3D063A9FAF021DA575ECF223" style="width: 200px;" /></div> <div class="col-sm-3 col-centered"><img alt="PacBio" src="/-/media/PacBio-CSP_logo.ashx?w=200&la=en&hash=1568DC13B370C032463997ED7BC5591C1FECF672" style="width: 200px;" /></div> <div class="col-sm-3 col-centered"><img alt="10x Genomics" src="/-/media/10x-Genomics-CSP_logo.ashx?w=200&la=en&hash=2584354CF0810D46E980E04C8C03461D332EE516" style="width: 200px;" /></div> <div class="col-sm-3 col-centered"><img alt="NanoString" src="/-/media/Images/Resources/NGS-Platforms-and-Specifications/NanoString_resized_logo.ashx?w=200&la=en&hash=A22CDFCF6A16A9884C633E8C2EF98B468325D130" style="width: 200px;" /></div> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><img alt="Nanopore" src="/-/media/ONT_ServiceCertified_logo-cut.ashx?w=200&la=en&hash=4140BDC9974099FF45B0BBB29FCC746FEDCFBCC7" style="width: 200px;" /></div> <div class="col-sm-3 col-centered"><img alt="Olink" src="/-/media/Olink-Certified-Service-Provider-cut.ashx?w=200&la=en&hash=A8C78860807705F8B01299DE5A728848734F53EF" style="width: 200px;" /></div> </div> <br /> <br /> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Related Services</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <a href='https://www.genewiz.com/en/Public/Services/Plasmid-DNA-Prep' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/15016-D_Plasmid-EZ_Plasmid-Preparation.ashx?h=111&la=en&w=211&hash=FA1A0FF4614AA48529F9258B2029B1E15D4CCADF" alt="Building" width="211" height="111" title="Plasmid DNA Prep Icon" /> </div> <div class="row"> <h3>Plasmid DNA Preparation</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='https://www.genewiz.com/en/Public/Services/Sanger-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/15016-D_Plasmid-EZ_Sanger-Sequencing.ashx?h=111&la=en&w=211&hash=D29F417A549DC8BDB26E1BAD224249B39C516D36" alt="Building" width="211" height="111" title="Sanger Sequencing Icon" /> </div> <div class="row"> <h3>Sanger Sequencing</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='https://www.genewiz.com/en/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Amplicon-EZ' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/15016-D_Plasmid-EZ_Amplicon-EZ.ashx?h=111&la=en&w=211&hash=15133D55BFAA12C674C5528B66411A4C583DAEBD" alt="Building" width="211" height="111" title="Amplicon EZ Icon" /> </div> <div class="row"> <h3>Amplicon-EZ</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <div class="process-v1 How-To-Order 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