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li > ul.mfn-megamenu-bg{box-sizing:border-box}#Top_bar .menu > li > ul.mfn-megamenu-bg{padding:20px 166px 20px 20px;background-repeat:no-repeat;background-position:right bottom}.rtl #Top_bar .menu > li > ul.mfn-megamenu-bg{padding-left:166px;padding-right:20px;background-position:left bottom}#Top_bar .menu > li > ul.mfn-megamenu-bg > li{background:none}#Top_bar .menu > li > ul.mfn-megamenu-bg > li a{border:none}#Top_bar .menu > li > ul.mfn-megamenu-bg > li > ul{background:none!important;-webkit-box-shadow:0 0 0 0;-moz-box-shadow:0 0 0 0;box-shadow:0 0 0 0}.mm-vertical #Top_bar .container{position:relative}.mm-vertical #Top_bar .top_bar_left{position:static}.mm-vertical #Top_bar .menu > li ul{box-shadow:0 0 0 0 transparent!important;background-image:none}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu{padding:20px 0}.mm-vertical.header-plain #Top_bar .menu > li > ul.mfn-megamenu{width:100%!important;margin:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > 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#menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;transform:unset}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}.header-simple #Top_bar #menu ul li.hover > 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ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:not(.menu-toggle):after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span{border-width:0!important}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}.menuo-no-borders.header-plain #Top_bar a#header_cart,.menuo-no-borders.header-plain #Top_bar a#search_button,.menuo-no-borders.header-plain #Top_bar .wpml-languages,.menuo-no-borders.header-plain #Top_bar a.action_button{border-width:0}.menuo-right #Top_bar .menu_wrapper{float:right}.menuo-right.header-stack:not(.header-center) #Top_bar .menu_wrapper{margin-right:150px}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank,body.under-construction.header-rtl.header-creative.header-open{padding-left:0!important;padding-right:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background-color:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar #menu{background-color:transparent}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{margin:0;width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}.menuo-arrows #Top_bar .menu > li.submenu > a > span:after{content:unset!important}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:left;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{content:none}#Header_creative #Top_bar .top_bar_right .top_bar_right_wrapper{flex-wrap:wrap;justify-content:center}#Header_creative #Top_bar .top_bar_right .top-bar-right-icon,#Header_creative #Top_bar .top_bar_right .wpml-languages,#Header_creative #Top_bar .top_bar_right .top-bar-right-button,#Header_creative #Top_bar .top_bar_right .top-bar-right-input{min-height:30px;margin:5px}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .contact_details{width:100%;text-align:center;margin-bottom:20px}#Header_creative #Action_bar .contact_details li{padding:0}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative #Action_bar .social-menu{float:none;text-align:center}#Header_creative #Action_bar .social-menu li{border-color:rgba(0,0,0,.1)}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}.header-open #Header_creative #Action_bar{display:block}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left;width:100%}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;z-index:203}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul.sub-menu li:not(:last-of-type) a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1240px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none;box-shadow:unset}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo,#Top_bar.is-sticky .custom-logo-link{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}.logo-sticky-width-auto #Top_bar.is-sticky #logo img.logo-sticky{width:auto}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky,.sticky-dark 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15px 30px 0 rgba(1,7,39,.13)}#mfn-gdpr .mfn-gdpr-content,#mfn-gdpr .mfn-gdpr-content h1,#mfn-gdpr .mfn-gdpr-content h2,#mfn-gdpr .mfn-gdpr-content h3,#mfn-gdpr .mfn-gdpr-content h4,#mfn-gdpr .mfn-gdpr-content h5,#mfn-gdpr .mfn-gdpr-content h6,#mfn-gdpr .mfn-gdpr-content ol,#mfn-gdpr .mfn-gdpr-content ul{color:#626262}#mfn-gdpr .mfn-gdpr-content a,#mfn-gdpr a.mfn-gdpr-readmore{color:#161922}#mfn-gdpr .mfn-gdpr-content a:hover,#mfn-gdpr a.mfn-gdpr-readmore:hover{color:#0089f7}#mfn-gdpr .mfn-gdpr-button{background-color:#006edf;color:#ffffff;border-color:transparent}#mfn-gdpr .mfn-gdpr-button:hover{background-color:#0089f7;color:#ffffff;border-color:transparent}@media only screen and ( min-width:768px ){.header-semi #Top_bar:not(.is-sticky){background-color:rgba(255,255,255,0.8)}}@media only screen and ( max-width:767px ){#Top_bar{background-color:#ffffff !important}#Action_bar{background-color:#007dc5 !important}#Action_bar .contact_details{color:#222222}#Action_bar .contact_details a{color:#0095eb}#Action_bar .contact_details a:hover{color:#007cc3}#Action_bar .social li a,#Action_bar .social-menu a{color:#bbbbbb!important}#Action_bar .social li a:hover,#Action_bar .social-menu a:hover{color:#777777!important}} form input.display-none{display:none!important} </style> <link rel='stylesheet' id='style-css' href='https://www.wyatt.com/wp-content/themes/betheme-child/style.css?ver=6.4.5' type='text/css' media='all' /> <style id='mfn-custom-inline-css' type='text/css'> @supports (-ms-ime-align: auto) { } span {font-family:"Open Sans", Arial, Tahoma, sans-serif !important;} @media only screen and (min-width: 1240px) { .header-classic #Header .top_bar_left {width:1306px;} #Top_bar .menu > li > a {padding:15px 5px;} #Top_bar.is-sticky .menu_wrapper .menu > li > a {padding:15px 5px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu{ padding-top:0px; padding-bottom:0px; width:92.5%!important; margin:0px 3%;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu .container {display:block;} /** Added 121024 **/ h1 { line-height: 44px !important; } .h2-underline {border-bottom: thick solid #ffb600; padding-bottom: 15px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu::before {content:' '; background:#fff; position:absolute; width:100vw; height:100%; margin-left: -50vw; left: 50%; } .mm-vertical #Top_bar .menu > li > .menu-overlap {z-index:1;background:rgba(0,0,0,0.4); position:absolute; width:100vw; height:100vh; margin-left: -50vw; left: 50%; cursor:hand; cursor:pointer;} .mm-vertical #Top_bar .menu > li.not-active a{color:#444444;} .mm-vertical #Top_bar .menu > li.not-active a::after {opacity:0;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title, .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title:hover {padding:5px 0px; background:transparent; color:#444444; text-transform:unset;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title:hover span {text-decoration:none ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu ul {background:transparent; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a {color:#007dc5; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a:hover {background:transparent;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a {padding:5px 0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a:hover {text-decoration:underline;} .mm-vertical #Top_bar .menu > li > ul {padding:20px 20px 20px 2px;} .mm-vertical #Top_bar .menu > li > ul a {padding:5px; border:0px; color:#007dc5;} .mm-vertical #Top_bar .menu > li > ul a:hover {background:transparent; } .mm-vertical #Top_bar .menu > li > ul a:hover span {text-decoration:underline ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li {padding-top:20px; padding-bottom:25px; border-right: 0px solid rgba(0, 0, 0, 0.05);} /*for parent title in sbmenu*/ .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 {padding-top:20px; line-height:30px; border-bottom:1px solid #ccc; font-weight:600;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 a{color:#444444;} /*for contact submenu*/ .mm-vertical #Top_bar .menu > li#menu-item-2437 li.mfn-megamenu-cols-2 li {} /*menu button css*/ .mm-vertical #Top_bar .menu > li#menu-item-5221 a span {background:#007dc5; color:#fff; border-radius:6px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-5221 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-5221 > a, #Top_bar.is-sticky .menu > li#menu-item-5221 > a span {line-height:30px; margin-top:0px;} .mm-vertical #Top_bar li#menu-item-6152 a {color:#000 !important; padding-top:0px !important; white-space:nowrap; color: #007dc5 !important; font-weight: 400;} /*set width of menu item*/ .mm-vertical #Top_bar li#menu-item-2341 {width:343px; padding-left: 80px;} .mm-vertical #Top_bar li#menu-item-2348 {width:278px;} .mm-vertical #Top_bar li#menu-item-2355 {width:288px;} .mm-vertical #Top_bar li#menu-item-2363 {width:240px;} .mm-vertical #Top_bar li#menu-item-2369 {width:541px; padding-left:70px;} .mm-vertical #Top_bar li#menu-item-2379 {width:340px;} .mm-vertical #Top_bar li#menu-item-2384 {width:280px;} .mm-vertical #Top_bar li#menu-item-2391 {width:350px; padding-left:80px;} .mm-vertical #Top_bar li#menu-item-2395 {width:310px;} .mm-vertical #Top_bar li#menu-item-2403 {width:310px;} .mm-vertical #Top_bar li#menu-item-2408 {width:210px;} .mm-vertical #Top_bar li#menu-item-5918 {width:487px; padding-left:115px;} .mm-vertical #Top_bar li#menu-item-5919 {width:387px;} .mm-vertical #Top_bar li#menu-item-5920 {width:280px;} .mm-vertical #Top_bar li#menu-item-2427 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-5235 {width:487px; padding-left:115px;} .mm-vertical #Top_bar li#menu-item-5765 {width:310px;} .mm-vertical #Top_bar li#menu-item-26131 {width:387px;} .mm-vertical #Top_bar li#menu-item-15082 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-15044 {width:397px;} } @media only screen and (max-width: 1239px) { #menu > ul > li > a, a.action_button, #overlay-menu ul li a { font-size: 15px; font-weight: 400; letter-spacing: 1px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar #menu ul li ul li a {font-size:16px;} .mm-vertical #Top_bar #menu ul li ul li ul li a {font-size:15px;} } /* Header */ .header-classic #Top_bar { border-bottom: 0px solid #e1e1e1;} .header-classic:not(.home) #Content { border-top: 0px solid #e1e1e1; } .header-classic.home .mfn-rev-slider { border-top: 1px solid #e1e1e1; } /*Action Bar*/ #Action_bar a {color:#fff;} /* Widget */ .widget-area:before { display: none; } .aside_left .widget-area { border: 0; } /* Icon box */ .icon_box.has_border { background: #fff; border: 1px solid #e1e1e1; padding: 40px 10%; } .icon_box.no_border {background: #fff; border:0px; padding: 40px 10%;} .icon_box.has_border:after {border:0px} /* Offer box */ .offer_box { background-color: #252525; padding: 20px 20px 5px; text-align: center; } .offer_box .offer_box_list { border-width: 1px 1px 0 1px; border-style: solid; border-color: #929292; font-weight: 700; font-size: 16px; letter-spacing: 2px; margin-bottom: 20px; } .offer_box .offer_box_list a { display: block; color: #fff; border-bottom: 1px solid #929292; padding: 18px 15px; word-wrap: break-word; } /* Footer */ /*#Footer { border-top: 1px solid #e1e1e1; }*/ #Footer .widget > h4 { font-size: 18px; font-weight: 300; margin-bottom: 20px; } #Footer .footer_copy { background: #465158; border-top: 0; } .before-footer {background: #f9f9f9;} #Footer .footer_copy .social { display: none; } /**/ .top-widgets-container {float:right} .top-widgets-container .widget {padding-bottom:0px; margin-bottom:0px; float:right;margin-top:0px;} .top-widgets-container .widget ul.menu{list-style:none; padding:0px;} .top-widgets-container .widget ul.menu li {float:left; padding:12px 0px;} .top-widgets-container .widget ul.menu li a{padding:0px 20px; border-right:1px solid #ccc; line-height:15px;} .top-widgets-container .widget ul.menu li:last-child a{border-right:0px;} .top-widgets-container .widget_search .icon-search-fine {font-size:24px; line-height:40px;} .top-widgets-container .widget_search input[type="text"] {width:82%; float:right; padding:5px 10px 5px 5px; border:0px; margin-top:7px; height:30px;min-height:30px;} #menu-top-navigation .cart a {padding:0px 20px; } #menu-top-navigation .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} .mobilecart {display:none; width:30px; float:right; margin-top:11px;} .mobilecart a { padding:0px; border:0px;} .mobilecart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; left:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { .top-widgets-container .widget {float:none;} .top-widgets-container .widget.widget_nav_menu {max-width:245px; margin:0px auto; clear:both;} .top-widgets-container .widget ul.menu li a {padding:0px 15px;} #menu-top-navigation .cart.menu-item-2449 {display:none;} #menu-top-navigation .cart a {padding:0px 10px;} .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .widget:after {display:none;} .top-widgets-container {float:none; margin:0px auto;} .top-widgets-container .widget_search input[type="text"] {width:80%;} .mobilecart {display:block;} .top-widgets-container .widget_search {width:254px; overflow:hidden;padding-bottom:20px; margin:0px auto;} .top-widgets-container .widget_search .searchform{width:210px; float:left;} } .icon-100 .image_wrapper img {width:150px; height:150px;} .down-heading, .heading {padding-left:17px;} @media only screen and (min-width:320px) and (max-width:767px) { .down-heading, .heading {padding:0px; text-align:center;} } .ui-tabs .ui-tabs-nav li {border-width:0px;} .page-id-2602 .ui-tabs .ui-tabs-nav li a {padding:14px 28px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li {border:0px; padding:5px 0px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {background-image:none; font-size:100%; color:#2991d6; padding:5px 10px 5px 20px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a:hover {text-decoration:underline;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a {color:#000; border-left:3px solid #2991d6; border-radius:0px !important; padding:5px 10px 5px 17px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:23%; border:0px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:76.7%; border:0px !important; padding-top:5px;} @media only screen and (min-width:768px) and (max-width:1239px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {font-size:100%;} } @media only screen and (min-width:320px) and (max-width:767px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:100%;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:100%; padding-top:15px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {-webkit-box-shadow:none;-moz-box-shadow:none;box-shadow:none;} } .ui-tabs .ui-tabs-nav li h3 {margin:0px; padding:0px;} .ui-tabs .ui-tabs-nav li h3 a { text-decoration: none; display: block; padding: 14px 30px !important; font-size: 100%; font-weight:initial; } .ui-tabs .ui-tabs-panel .icon_box {padding-bottom:2px;} .ui-tabs .ui-tabs-panel .column.one-fourth {width:25%;} .ui-tabs .ui-tabs-panel .column.one-second, .ui-tabs .ui-tabs-panel .column.one-fourth, .ui-tabs .ui-tabs-panel .column.one-third, .ui-tabs .ui-tabs-panel .column.two-third{margin-left:0px; margin-right:0px;} @media only screen and (min-width: 960px) and (max-width: 1239px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:10px 20px !important;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:5px 10px !important;} } /* Make Nav Subheadings unclickable */ .unclickable > a:hover { cursor: default;} .wp-caption-text { background: transparent; color: #075c97; font-size:90%;} .ls-wrapper h3 a {color: #075c97;} .column_sidebar_widget {float:none;} /*app notes*/ ul.clients.appnotes .client_wrapper {text-align:left; height:auto !important; line-height:auto !important; overflow:hidden;} ul.clients.appnotes li:nth-child(2n+1) {clear:both;} ul.clients.appnotes .client_wrapper img { position:relative; left:auto !important; top: auto !important; float:left; -webkit-transform: none!important; -moz-transform: none!important; transform: none!important; padding-right:20px; width:auto!important; } ul.clients.appnotes .client_wrapper h3 { line-height:20px; font-size:16px; letter-spacing:1px; padding-top:10px;} ul.clients.appnotes .client_wrapper a:hover {text-decoration:none;} @media only screen and (max-width: 640px) { ul.clients.appnotes li {width:100% !important; clear:both;} } .pager {padding-top:20px;} .pager .page-numbers {border:1px solid #ccc; padding:3px 5px;} .pager .prev.page-numbers, .pager .next.page-numbers {border:0px;} .alignleft {margin:3px 10px 10px 0px;} dl {clear:both;} .team.team_vertical img.scale-with-grid {width:100%;} .column_placeholder {margin-bottom:0px;} .icon-height img{max-height:50px;} .single .post-header {margin-left:0px; margin-right:0px;} .single .section-post-header .single-photo-wrapper.image {display:none;} .widget_mfn_menu ul li.menu-item-has-children > a::after {content: '\e868'; font-family: "mfn-icons"; right:2px; position:absolute; width:20px; height:15px; color:#ccc; } table th ul, table td ul {list-style-position:inside !important;} .sticky-white #Top_bar.is-sticky, .sticky-white #Top_bar.is-sticky #menu, #Top_bar.is-sticky {background:#fff; opacity:1;} /*Webinars latest posts*/ .parent-pageid-3663 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-3663 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-3663 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-3663 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .parent-pageid-3663 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-3663 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars*/ .page-id-2653 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-2653 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-2653 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-2653 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-2653 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .page-id-2653 .Latest_news ul li .desc_footer .date {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-2653 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-2653 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-2653 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Newsletter Archive*/ .page-id-3661 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-3661 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-3661 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-3661 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-3661 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li.category-newsletter-archive .desc h5 a{color:#007dc5;} .page-id-3661 .Latest_news ul li .desc_footer .date {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-3661 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-3661 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-3661 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Ask the Expert*/ .page-id-5348 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-5348 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-5348 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-5348 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc h5{margin-bottom:0px;} .page-id-5348 .Latest_news ul li.category-ask-the-expert .desc h5 a{color:#007dc5;} .page-id-5348 .Latest_news ul li .desc_footer {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .date {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-5348 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-5348 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-5348 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; font-weight:normal; line-height:20px;} /*accordion title as h3*/ .accordion .question .title h3 {margin-bottom:0px; } .accordion .question.active .title h3{color:#2991d6} /*column padding*/ .column, .columns {margin:0px 10px 40px;} .accordion .answer .one-second.column {width:47%;} .wrap .one-second.column {width:47%;} /*.column_column ul li {margin-bottom:2px;}*/ .single .post-header {margin-bottom:10px;} /* Appnote seach box*/ .widget_appnote_search_widget {clear:both; display:block; overflow:hidden;} .widget_appnote_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_appnote_search_widget .searchform {display:inline-block; float:left;} .widget_appnote_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_appnote_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_appnote_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_appnote_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*flat box*/ .flat_box .photo_wrapper .icon {display:none;} .flat_box .photo_wrapper img.photo {transform: translateX(0px)} .flat_box .desc_wrapper {margin-left:0px;} /*center images at user profile*/ .page-id-2504 .image_frame .image_wrapper {text-align:center} .page-id-2504.if-overlay .image_frame:hover .image_wrapper img, .page-id-2504.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .if-overlay .image_frame .image_wrapper .image_links {display:none;} /* Changes for ClickDimensions submit button */ a.button_theme, a.tp-button.button_theme, button, input[type="submit"], input[type="reset"], input[type="button"] { background-color: #0065a4 !important; color: #ffffff !important; font-family: inherit !important; /*padding:0px;*/ } /*isotope wrapper*/ .blog_wrapper.isotope_wrapper .post-item .post-footer {background:none;} .blog_wrapper.isotope_wrapper .post-item .post-footer .post-links{border:0px;} .blog_wrapper.isotope_wrapper .grid .post-photo-wrapper {border:0px;} /* product page*/ .page-id-2582 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-2582 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-2582 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .uk-margin-small-right {vertical-align:middle; margin-top:-2px;} .page-id-2647 .ui-tabs .ui-tabs-panel .column.one-second {margin-bottom:0px;} .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {border-color:transparent;} @media only screen and (min-width: 960px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {width:12%;} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:88%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper { width:25%} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:75%;} } @media only screen and (min-width: 768px) { .page-id-2640 .column.column_pricing_item {margin:0px 1% 40px;} } /* contact form 7 submit button*/ .wpcf7-form-control.wpcf7-submit {padding:10px 30px;} .wpcf7 .red {color:#990000;} .wpcf7 span.wpcf7-list-item {display:block; margin:0px;} .wpcf7 label {font-weight:400;} #lostusername {border:1px solid #ddd; padding:30px 20px 0px;} #lostusername .input {width:100%;} #lostusername .button {padding:10px 20px;} .ur-frontend-form button[type="submit"], .ur-frontend-form input[type="submit"] {float:none;} .page-id-2690.if-overlay .image_frame:hover .image_wrapper img, .page-id-2690.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-2690 .team .desc_wrapper hr {display:none;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-2690 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* webinars seach box*/ .widget_webinars_search_widget {clear:both; display:block; overflow:hidden;} .widget_webinars_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_webinars_search_widget .searchform {display:inline-block; float:left;} .widget_webinars_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_webinars_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_webinars_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_webinars_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*Recent Webinars nav issue fix*/ .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a:after {display:none;} /*login-in nav issue fix*/ .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* Contact Customer Support nav issue fix*/ .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* custom seach box*/ .search #Content .searchform {display:inline-block; float:left;} .search #Content .searchform h4{clear:both; padding:10px 0px; margin:0px;} .search #Content .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .search #Content .searchform select {-webkit-appearance:menulist; padding:7px 10px;} .search #Content .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .search #Content .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .search #Content .searchform input[type=submit] {margin:0px; margin-top:15px;} } .wpcf7-form .anr_captcha_field {position:absolute;} /*newsletter archive tables remove border*/ .post.category-newsletter-archive .post-wrapper-content table td {box-shadow:none; border:0px;} .be-ix-link-block {max-width:750px; text-align:center; margin:0px auto; padding-top:20px;} #Footer .widgets_wrapper .column {margin:0px 9px 0px;} @media only screen and (min-width: 768px) and (max-width: 959px) { #Footer .widgets_wrapper .column {margin:0px 5px 0px;} } /*Login page popup */ .page-id-5451.logged-in #pum-8728 {display:none !important;} #cookie-law-info-bar a {color:#fff; text-decoration:underline;} #cookie-law-info-bar a:hover {text-decoration:none;} #cookie-law-info-bar .cli-plugin-button {text-decoration:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { #cookie-law-info-again{display:none !important;} } /*After Multi Language SETUP*/ #Action_bar .column {overflow:visible;} .top-widgets-container .widget_search input[type="text"] {margin-top:5px;} .widget.widget_nav_menu::after, .widget.widget_icl_lang_sel_widget::after, .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .wpml-ls-legacy-dropdown-click {margin-top:6px; } .wpml-ls-legacy-dropdown-click a, .wpml-ls-legacy-dropdown-click a:hover, .wpml-ls-legacy-dropdown-click a:focus, .wpml-ls-legacy-dropdown-click .wpml-ls-current-language:hover>a {color:#626262 !important; border:0px;text-decoration:none;} .wpml-ls-legacy-dropdown-click .wpml-ls-sub-menu {z-index:999;} .top-widgets-container h2.title {display:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { .wpml-ls-legacy-dropdown-click {margin:6px auto 0px; } .top-widgets-container .widget_search {clear:both;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .wpml-ls-legacy-dropdown-click {width:auto;} } #menu-top-navigation-ch .cart a {padding:0px 20px; } #menu-top-navigation-ch .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ch .cart.menu-item-2449 {display:none;} #menu-top-navigation-ch .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-13087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-13087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-13087 > a, #Top_bar.is-sticky .menu > li#menu-item-13087 > a span {line-height:30px; margin-top:0px;} } .post-9911 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .post-9911 span.wpcf7-form-control-wrap{margin-bottom:20px;} .post-9911 .wpcf7-list-item-label{color:#000000;} .post-9911 .unique .one-third.column{width:30.333% !important;} .post-9911 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .post-9911 .wrap .one-second.column{width:32%;} .post-9911 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .post-9911 .wpcf7 span.wpcf7-list-item{width: 100%;} .post-9911 .unique .one-third.column{width:100% !important;} .post-9911 .wrap .one-second.column{width:100%;} .post-9911 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*remove meta from search-results*/ .search.search-results .post-item .post-meta {display:none;} .search.search-results .post-item .post-desc {padding-left:0px;} /*footer update*/ /*#Footer .widgets_wrapper {padding-bottom:10px;} #Footer .widgets_wrapper .column {margin-bottom:10px;} #Footer #custom_html-4 > h4 {text-align:center;} #Footer #custom_html-14 > h4 {text-align:center;} #Footer #custom_html-9 {max-width:160px; margin:0px auto; clear:both; padding-top:25px;} .footer-resources {max-width:80%; margin:0px auto; } .footer-resources li {float:left; line-height:28px;} .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:12%; clear:both;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:28%;} html[lang="zh-hans"] .footer-resources li:nth-child(1), html[lang="zh-hans"] .footer-resources li:nth-child(6){width:15%; clear:both;} html[lang="zh-hans"] .footer-resources li:nth-child(2), html[lang="zh-hans"] .footer-resources li:nth-child(7){width:25%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:17%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:18%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:25%;} @media only screen and (min-width:320px) and (max-width:1239px) { .footer-resources {max-width:60%; } .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:50%; clear:none;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:50%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:50%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:50%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:50%;} } @media only screen and (min-width:320px) and (max-width:767px) { .footer-resources {max-width:100%; } #Footer #custom_html-4 > h4 {text-align:left;} #Footer #custom_html-14 > h4 {text-align:left;} #Footer #custom_html-9 {max-width:100%;} } */ .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display:inline-block; text-decoration: none; padding: 0px 20px !important; border:0px; text-transform: uppercase; line-height:30px; font-size: 15px; font-weight: 400;letter-spacing: 1px;} @media only screen and (min-width:320px) and (max-width:767px) { .tabs_vertical.ui-tabs .ui-tabs-nav li h3, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active h3 {text-align:center;} .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover {margin:20px auto;} } /*Webinars latest posts zh-hans*/ .parent-pageid-11725 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-11725 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-11725 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-11725 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .parent-pageid-11725 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-11725 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars zh-hans*/ .page-id-11764 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-11764 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-11764 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-11764 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-11764 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .page-id-11764 .Latest_news ul li .desc_footer .date {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-11764 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-11764 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-11764 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Request Product Quote zh-hans*/ .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12227 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12227 .wpcf7-list-item-label{color:#000000;} .page-id-12227 .unique .one-third.column{width:29% !important;} .page-id-12227 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12227 .wrap .one-second.column{width:32%;} .page-id-12227 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12227 .unique .one-third.column{width:100% !important;} .page-id-12227 .wrap .one-second.column{width:100%;} .page-id-12227 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Request Product Info zh-hans*/ .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12612 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12612 .wpcf7-list-item-label{color:#000000;} .page-id-12612 .unique .one-third.column{width:29% !important;} .page-id-12612 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12612 .wrap .one-second.column{width:32%;} .page-id-12612 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12612 .unique .one-third.column{width:100% !important;} .page-id-12612 .wrap .one-second.column{width:100%;} .page-id-12612 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Team in zh-hans*/ .page-id-12002.if-overlay .image_frame:hover .image_wrapper img, .page-id-12002.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-12002 .team .desc_wrapper hr {display:none;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-12002 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* product page in zh-hans*/ .page-id-12156 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-12156 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-12156 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } /*Recent Webinars nav issue fix for zh-hans*/ .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a:after {display:none;} .large h3{font-size:22px; line-height:30px;} .small {margin-left:25px;} sup {display:inline-block; margin-top:-4px;} sub {display:inline-block; margin-bottom:-4px;} sup, sub {display:inline-table;} .ui-tabs-panel .one-second.column {width:50%;} .parent-pageid-2582 .ui-tabs-panel td {text-align:left !important;} .ls-borderlessdark3d .ls-shadow { display: none !important;} #menu-top-navigation-de .cart a {padding:0px 20px;} #menu-top-navigation-de .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-de .cart.menu-item-14543{display:none;} #menu-top-navigation-de .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-15087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-15087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-15087 > a, #Top_bar.is-sticky .menu > li#menu-item-15087 > a span {line-height:30px; margin-top:0px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar li#menu-item-12984 a {color:#000 !important; padding-top:20px !important;} .mm-vertical #Top_bar li#menu-item-14978 a {color:#000 !important; padding-top:20px !important;} } #Footer #custom_html-15 > h4 {text-align:center;} /* product page German */ .page-id-14845 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-14845 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-14845.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-14295.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-14845 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .parent-pageid-14845 .ui-tabs-panel td {text-align:left !important;} .button_align.align_left .button {text-align:left; padding:10px 0;} .button_align.align_left .as_tab {font-size: 18px; line-height: 20px; font-weight: 400; background:#fff; letter-spacing: 0px; color: #2991d6 !important; padding: 5px 10px 5px 20px;margin:0; } .button_align.align_left .button.as_tab:hover {text-decoration:underline !important; background-color:#fff!important;} .button_align.align_left .button.as_tab:after {display:none;} .button_align.align_left .button.as_tab.active {color: #000; border-left: 3px solid #2991d6; border-radius: 0px !important; padding: 5px 10px 5px 17px !important;} .button_align.align_left .button.as_tab .button_label {margin:0!important;} .button_align.align_left .button.as_tab_btn { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display: inline-block; text-decoration: none; padding: 0px 20px !important; border: 0px; text-transform: uppercase; line-height: 30px; font-size: 15px; font-weight: 400; letter-spacing: 1px; } /*Japan language*/ #Footer #custom_html-22 > h4 { text-align: center; } #menu-top-navigation-ja .cart a {padding:0px 20px;} #menu-top-navigation-ja .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ja .cart.menu-item-2449 {display:none;} #menu-top-navigation-ja .cart a {padding:0px 10px;} } .mm-vertical #Top_bar .menu > li#menu-item-18403 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-18403 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-18403 > a, #Top_bar.is-sticky .menu > li#menu-item-18403 > a span {line-height:30px; margin-top:0px;} @media only screen and (min-width: 1240px) { #Top_bar #menu-german-main-menu > li > a span:not(.description) { padding: 0 3px; font-size:14px; } } body, ul.timeline_items, .icon_box a .desc, .icon_box a:hover .desc, .feature_list ul li a, .list_item a, .list_item a:hover, .widget_recent_entries ul li a, .flat_box a, .flat_box a:hover, .story_box .desc, .content_slider.carousel ul li a .title, .content_slider.flat.description ul li .desc, .content_slider.flat.description ul li a .desc, .post-nav.minimal a i { color: #333333 !important; } .column_column ul, .column_column ol, .the_content_wrapper:not(.is-elementor) ul, .the_content_wrapper:not(.is-elementor) ol { color: #333333 !important; } .hero { margin-top: 20px; } .bg-hero { background: radial-gradient(circle at 100% 0,#ebf1f2 0% 30%,#0e81a7 100%); } .hero-full { min-height: 450px; background-color: rgba(0, 0, 0, 0.5); } .column, .columns { margin: 0px 8px 40px; } .image-container { position: relative; display: inline-block; /* oder 'block' je nach Layout */ } .ribbon { position: absolute; top: 10px; /* Abstand von oben */ left: -20px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #FF5733; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 5px 15px; /* Innenabstand */ transform: rotate(-0deg); /* Dreht das Ribbon */ transform-origin: 0 0; /* Setzt den Drehpunkt */ font-size: 14px; /* Schriftgröße */ box-shadow: 2px 2px 5px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .event-badge { position: relative; margin-right: 10px; border-radius: 6px; top: 0px; /* Abstand von oben */ left: 0px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #007dc5; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 2px 8px; /* Innenabstand */ font-size: 10px; /* Schriftgröße */ box-shadow: 1px 1px 2px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .btn-default { background: #007dc5; color: #fff; border-radius: 6px; line-height: 40px; margin-top: 8px; margin-left: 20px; padding: 0px 10px; } .wpcf7 form.sent .wpcf7-response-output { color: #ffffff; background-color: #46b450; } .jumpto { padding-top: 100px; /* Adjust this value to match your header's height */ margin-top: -100px; /* This should be the same value as padding-top but negative */ } .post-item { margin-bottom: 40px; position: relative; float: left; width: 100%; border: 1px solid #ccc; padding: 20px; background-color: #f8f8f8; } .post-links { background-color: #007dc5; border: 1px solid #ccc; padding: 5px 20px; } #field_1_18, #field_1_113, #field_1_20, #field_1_21, #field_1_22, #field_1_23, #field_1_24, #field_1_25, #field_1_26, #field_1_27, #field_1_49, #field_1_51, #field_1_52, #field_1_53, #field_1_54, #field_1_56, #field_1_57, #field_1_58, #field_1_59, #field_1_60, #field_1_62, #field_1_63, #field_1_64, #field_1_65, #field_1_67, #field_1_68, #field_1_70, #field_1_72, #field_1_98, #field_1_99, #field_1_100, #field_1_101, #field_1_102, #field_1_103, #field_1_104, #field_1_107, #field_1_108, #field_1_122, #field_1_123, #field_1_124, #field_1_125, #field_1_126, #field_1_127, #field_1_128, #field_1_136 { background-color: #E8E8E8; padding: 20px 30px; border: 1px solid #C4C4C4; border-bottom: 1px solid #C4C4C4; margin-top: -20px; } #field_1_73, #field_1_74, #field_1_75, #field_1_76, #field_1_77, #field_1_78, #field_1_82, #field_1_83, #field_1_84, #field_1_85, #field_1_86, #field_1_92, #field_1_93, #field_1_94, #field_1_95, #field_1_129, #field_1_132, #field_1_133, #field_1_134, #field_1_135 { background-color: #F8F8F8; padding: 20px 50px; border-bottom: 1px solid #C4C4C4; border-right: 1px solid #C4C4C4; border-left: 1px solid #C4C4C4; margin-top: -40px; } #field_1_114, #field_1_115, #field_1_116, #field_1_117, #field_1_118, #field_1_119, #field_1_120, #field_1_121, #field_1_130 { background-color: #8ed1fc; padding: 20px 50px; border-bottom: 1px solid #C4C4C4; border-right: 1px solid #C4C4C4; border-left: 1px solid #C4C4C4; margin-top: -40px; } .gfield_label { font-weight: 800 !important; font-size: 16px !important; } .post-more, .post-more:hover, .icon-doc-text { color: #ffffff; } .icon_box.has_border { background: #fff; border: 0px solid #e1e1e1 !important; padding: 40px 10%; padding-bottom: 40px; } #Footer .widget > h4 { font-size: 18px; font-weight: 600; margin-bottom: 20px; } /* new css */ .mcb-column-inner { margin: 0px; overflow:visible;} .one-sixth.column { width: 14.666%; } /* 1/6 */ .one-fifth.column { width: 18%; } /* 1/5 */ .one-fourth.column, .four.columns { width: 23%; } /* 1/4 */ .one-third.column { width: 31.333%; } /* 1/3 */ .two-fifth.column { width: 38%; } /* 2/5 */ .one-second.column { width: 48%; } /* 1/2 */ .three-fifth.column { width: 58%; } /* 3/5 */ .two-third.column { width: 64.666%; } /* 2/3 */ .three-fourth.column { width: 73%; } /* 3/4 */ .four-fifth.column { width: 78%; } /* 4/5 */ .five-sixth.column { width: 81.333%; } /* 5/6 */ .one.column { width: 98%; } /* 1/1 */ .wpcf7-form .column { margin-bottom: 10px;} .wpcf7-form .column { padding: 0px;} .wpcf7-form .column p{ margin: 0px;} .column-margin-0px.column { margin-bottom: 0 !important;} .column-margin-10px.column { margin-bottom: 0px !important;} .column-margin-20px.column { margin-bottom: 0px !important;} .page-pager, .post-pager { margin-bottom: 0!important;} .column.column_divider { margin-bottom: 0!important; height: auto!important;} #Header .container {padding-left:0; padding-right:0;} .accordion .question > .title > .acc-icon-plus, .accordion .question.active > .title > .acc-icon-minus { display: block;} .accordion .question > .title > .acc-icon-plus, .accordion .question .title > .acc-icon-minus {left:15px; top:16px;} .mcb-wrap-inner {display:block;} @media only screen and (max-width: 767px) { .section_wrapper .mcb-wrap, .section_wrapper .column, .container .column { width:100%!important; } } @media only screen and (min-width: 768px) { .be-ix-link-block .be-related-link-container .be-label {flex:0 0 17%;} .be-ix-link-block .be-related-link-container .be-list {flex:0 0 83%;} } ul.clients.appnotes .client_wrapper a, ul.clients.appnotes .client_wrapper .gs-wrapper { justify-content:left; } /*Top bar search*/ #Action_bar #global-searchfield-container .addsearch-searchfield input[type=search].icon {margin-bottom:0px; margin-top:4px; padding:5px 5px 5px 40px!important; height:30px;} #Action_bar #global-searchfield-container .addsearch-searchfield button {display:none;} #Action_bar .adds-components {padding:0px;} /*Top Shadow */ .header-classic #Top_bar { border-bottom: 0px solid #e1e1e1; box-shadow: rgba(0, 0, 0, 0.15) 0px 1.95px 2.6px; margin-bottom: 1px; } .sticky-submenu { position: -webkit-sticky; /* Für Safari */ position: sticky; top: 80px; z-index: 9999; /*background-color: #fff;*/ padding: 10px; /*border-bottom: 1px solid #ccc;*/ text-align: center; } .sticky-submenu ul { list-style-type: none; margin: 0; padding: 0; } .sticky-submenu ul li { display: inline; margin: 0 15px; } .sticky-submenu ul li a { text-decoration: none; /*color: #000;*/ color:#fff; padding: 5px; transition: border-bottom 0.3s; } .sticky-submenu ul li a.active, .sticky-submenu ul li a:hover { /*border-bottom: 2px solid #000;*/ border-bottom: 2px solid #fff; } .page-section { padding: 50px; margin-top: 50px; } .anchor-offset { display: block; position: relative; top: -60px; /* Höhe des sticky-submenu */ } .copyright a {padding:0px 7px;} .before-footer h4 {font-size:18px;} .before-footer .button {border-radius:4px;} .footer-button { padding: 10px 5px; border-radius: 5px; text-align: center; display: inline-block; width: 180px; border:1px solid #fff !important;} #Footer .widget_nav_menu ul { list-style-type: none; padding: 0px; background: none;} #Footer .widget_nav_menu ul a{padding:0px;} #Footer .widget_nav_menu {margin:20px 0px;} #Footer .one-fifth {width:16%;} #Footer .one-fifth:first-child {width:27%;} .social .icon_bar, .social .icon_bar span {width:auto !important; height:auto!important; line-height:0px!important; background-color:transparent; border:0px !important; color:#fff; font-size:24px; -webkit-box-shadow:none;box-shadow:none;} .social a:hover.icon_bar span { -webkit-transform: translateY(0%); -moz-transform: translateY(0%); transform: translateY(0%); } /*30aug2024*/ table tr:first-child td {box-shadow:none;} .btn_radius {border-radius: 4px;} #Top_bar .menu > li > a span:not(.description) {padding:0px 3px;} @media only screen and (min-width: 1300px) { #Top_bar .menu > li > a span:not(.description) {padding:0px 10px;} #Top_bar.is-sticky .menu > li > a span {padding:0px 15px;} } /** Added 11/14/24 **/ .scroll { text-decoration: none; transition: color 0.3s ease, font-weight 0.3s ease; } .scroll.active { font-weight: bold; } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-5221 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-15087 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-13087 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-18403 a span { background: #13b5ea !important; } } /** Added 11/18/24 **/ .header-section { border-bottom: 8px solid #fdbb30 !important; } .header-section .mcb-background-overlay{ } .solution-box { padding: 20px; border: 4px solid #EBEDEF; min-height: 150px; } .image_frame:hover .image_wrapper .mask { display:none; } .image_frame:hover .image_wrapper img, .image_frame:focus .image_wrapper img { top: 0 !important; -webkit-transform: scale(1) !important; -moz-transform: scale(1) !important; -o-transform: scale(1) !important; transform: scale(1) !important; -webkit-transition: none !important; transition: none !important; } .section-headline { border-bottom: thick solid #fdbb30; padding-bottom: 15px; font-size: 26px; } /** Page CTA Elements **/ .responsive-container { display: flex; flex-wrap: nowrap; border: 4px solid #EBEDEF; padding: 20px; align-items: flex-start; gap: 20px; width: 100%; } .responsive-container img { width: 100px; height: auto; flex-shrink: 0; } .responsive-container div { flex: 1; } /* Breakpoint for mobile screens */ @media (max-width: 768px) { .responsive-container { flex-wrap: wrap; } .responsive-container img { width: 100%; margin-bottom: 20px; } .responsive-container div { flex: 1 1 100%; text-align: center; } } .cta-box-btn { display: inline-block; align-items: center; padding: 6px 20px; color: white; text-decoration: none; border-radius: 5px; font-size: 14px; width: fit-content; background-color: #0065a4 !important; transition: background-color 0.3s ease, transform 0.2s ease; } .cta-box-btn:hover { background-color: #0056b3; color: white; text-decoration: none; } .cta-box-icon { margin-right: 5px; } /* Sidebar-Button-Styling */ .sidebar-btn { display: block; width: 100%; text-align: center; padding: 10px 20px; box-sizing: border-box; background-color: #13b5ea !important; font-size: 16px; color: white; border-radius: 5px; transition: background-color 0.3s ease, transform 0.2s ease; } .sidebar-btn:hover { background-color: #0065a4 !important; color: white; text-decoration: none; } /*LS nav style*/ html body .ls-bottom-nav-wrapper a, #ls-global .ls-bottom-nav-wrapper a {background-image:none; border-radius:20px; background-color:#999; margin:3px; width:15px; height:15px;} html body .ls-bottom-nav-wrapper a.v, 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class="searchform" action="https://www.wyatt.com/" > <div><input type="text" placeholder="Search..." value="" name="s" id="s" /> <a href="javascript:document.getElementById('searchform2').submit();" class="icon-search-fine"></a> </div> </form><div class="mobilecart"><a href="https://store.wyatt.com/"></a></div> </div> </div> </div> </div> </div> </header> </div>  <div id="Content" role="main"> <div class="content_wrapper clearfix"> <main class="sections_group"> <div class="entry-content" itemprop="mainContentOfPage"> <div class="mfn-builder-content mfn-default-content-buider"><section class="section mcb-section mfn-default-section mcb-section-qzvj6f7w8" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-qzvj6f7w8"><div class="wrap mcb-wrap mcb-wrap-e15zzrwjm one tablet-one laptop-one mobile-one valign-top clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-e15zzrwjm mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-1ykt11nq2 one laptop-one tablet-one mobile-one column_column column-margin-20px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-1ykt11nq2 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""></div></div></div></div></div></div></section><section class="section mcb-section mfn-default-section mcb-section-37c5e0c82" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-37c5e0c82"><div class="wrap mcb-wrap mcb-wrap-3ecc2f282 one tablet-one laptop-one mobile-one valign-top clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-3ecc2f282 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-vzcl9gj88 one laptop-one tablet-one mobile-one column_column column-margin-10px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-vzcl9gj88 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h1>Dynamic Light Scattering (DLS)</h1></div></div></div><div class="column mcb-column mcb-item-94df23609 one laptop-one tablet-one mobile-one column_tabs" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-94df23609 mcb-item-tabs-inner"><div class="jq-tabs tabs_wrapper tabs_vertical"><ul><li><h3><a href="#dls-1">Introduction</a></h3></li><li><h3><a href="#dls-2">System</a></h3></li><li><h3><a href="#dls-3">Applications</a></h3></li><li><h3><a href="#dls-4">Workflow</a></h3></li><li><h3><a href="#dls-5">Products</a></h3></li><li><h3><a href="#dls-6">Videos</a></h3></li><li><h3><a href="#dls-7">Resources</a></h3></li><li><h3><a href="#dls-8">Services & Support</a></h3></li><li><h3><a href="#dls-9">REQUEST INFO</a></h3></li></ul><div id="dls-1" ><h2 style="color:#075c97;">Size distributions measured quickly and easily</h2> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Widely used in biochemistry, biotechnology and pharmaceutical development, dynamic light scattering (DLS) is popular wherever the size distributions of nanomaterials need to be measured quickly and easily for a wide range of analytes such as:</p> <ul> <li>Proteins, peptides, nucleic acids and their aggregates</li> <li>Nano-drug delivery systems, e.g., liposomes, lipid nanoparticles (LNPs), micelles, gene vectors and viruses</li> <li>Nanoparticles and nanoplastics</li> <li>Polymers and hydrogels</li> <li>Compound aggregates</li> </ul> <p>Whether measuring directly in microwell plates, in conventional cuvette-based format, automated or online in conjunction with a MALS detector, Wyatt offers instruments and options to fit your needs.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img fetchpriority="high" alt="sec-mals" src="/wp-content/uploads/2021/10/Size-Chart-DLS.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="accordion"><div class="mfn-acc accordion_wrapper "> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>What is DLS?</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 10px 0px 10px"> <p>Dynamic light scattering is a powerful, yet easy to use technique to measure the hydrodynamic size and related parameters of macromolecules and nanoparticles in solution, without the need for chromatographic separation. Assess sample properties such as:</p> <ul> <li>Size and purity of proteins</li> <li>Soluble and insoluble aggregates up to 5000 nm for small molecules, bio-macromolecules and nanoparticles</li> <li>Particle concentration of nanomedicines</li> <li>Thermal, colloidal and chemical stability of protein formulations</li> <li>Conformational changes in macromolecules</li> </ul> </div> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="" src="/wp-content/uploads/2021/10/Monomodal-Graph.jpg" title="DLS" width="70%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Why DLS?</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 10px 0px 10px"> <p>DLS is an ideal screening tool for biopharmaceutical formulations and other nanomaterials. DLS offers unique benefits such as:</p> <ul> <li><b>Fast:</b> get data in less than a minute</li> <li><b>Full nanometer size range:</b> from 0.5 nm to 2.5 µm in a single measurement, with a resolution up to a factor of 3-5 in size.</li> <li><b>Non-destructive and fully recoverable:</b> Samples may be transferred to other assays</li> <li><b>Low sample volume:</b> Only 2 µL in the DynaPro NanoStar using quartz cuvettes, 4 µL in disposable cuvettes or standard microwell plates in the DynaPro Plate Reader</li> <li><b>No shearing or filtering:</b> always measure what’s in your solution</li> <li><b>Versatility:</b> Beyond size and size distributions, measure other properties such as stability, stress-induced aggregation and molecular interactions </li> <li><b>Advanced properties</b>: Measure particle concentration, nonspecific interactions (<i>A</i><sub>2</sub> or <i>B</i><sub>22</sub>) and differentiate between conformational changes and aggregation with the static light scattering option in the NanoStar and Plate Reader</li> <li><b>Pre-screening</b>: Ideal to check sample quality before running other expensive and time-consuming biophysical methods such as TEM, AUC, SAXS/SANS, FFF and SEC.</li> </ul> <p>For in-depth characterization of high-resolution size distributions and other physical properties, <a href="/solutions/techniques/sec-mals-molar-mass-size-multi-angle-light-scattering.html">SEC-MALS</a> and <a href="/solutions/techniques/fff-mals-characterization-of-nanoparticles-colloids-macromolecules.html">FFF-MALS</a> offer complementary and orthogonal techniques to DLS.</p> </div> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="" src="/wp-content/uploads/2023/08/DLS-graph-updated.jpg" title="DLS"></p> <p align="left" style="color:#075c97;font-size:14px;padding-left:15px">DLS size distribution of a mixture of adeno-associated viruses (AAV) and aggregates. The intensity-weighted distribution, shown, highlights aggregates; the graph may be converted to a mass-weighted distribution, which would indicate that the true aggregate content is relatively much lower. The particle concentration of each population is calculated by combining DLS with static light scattering (SLS). </p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Who uses DLS?</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>DLS is used to analyze samples in the nanometer size range, by:</p> <ul> <li><b>Biopharmaceutical scientists</b> for characterization and process development of gene therapies, vaccines and biologics such as monoclonal antibodies</li> <li><b>Pharmaceutical technologists</b> for developing nanodrugs such as micelles, liposomes, nanocrystals and polymeric controlled-release particles</li> <li><b>Molecular biology and nanomedicine researchers</b> for studying liposomes, lipid nanoparticles and extracellular vesicles</li> <li><b>Polymer analytical chemists</b> for characterizing biopolymers, thermo-responsive polymers, polymer solubility and polymer latex particles</li> <li><b>Environmental scientists</b> to study nanoplastics, tracer particles and toxic materials in the environment</li> <li><b>Nanoparticle engineers</b> for advanced materials</li> </ul> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2022/05/Sophia-Student-Lab-600.jpg"> </p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> </div></div> </div><div id="dls-2" ><h2 style="color:#075c97;">Versatile technology</h2> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>While dynamic light scattering instruments are generally used for single samples in batch or standalone configuration, they may also be employed for flow-injection analysis and in conjunction with chromatography and MALS detectors. Even more, with the <a href="/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html">DynaPro<sup>™</sup> Plate Reader</a>, DLS measurements can be performed <i>in situ</i> in microwell plates.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2021/10/DynaPro-Plate-Reader-III-Plate-Pipette.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="accordion"><div class="mfn-acc accordion_wrapper "> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>How does DLS work?</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>DLS determines the hydrodynamic size of a nanoparticle in solution by measuring the time scale of Brownian motion. The sample is illuminated with a laser beam, and light waves scattered from the particles interfere constructively and destructively as the particles diffuse randomly. The resulting intensity fluctuations in the light scattering detector are analyzed to determine a translational diffusion coefficient, which is converted to hydrodynamic radius via the Stokes-Einstein relationship (see sidebar). The only necessary input parameters are solution viscosity and refractive index.</p> <p>DLS is a technique that can be readily applied for sizing unknown samples:</p> <ul> <li>Solvent viscosity and refractive index are generally well-known and tabulated. Moreover, <a href="/products/software/dynamics.html">DYNAMICS<sup>™</sup></a> software contains an extensive, built-in solvent library with the most common solvents and buffer systems, including the option to create custom solvents.</li> <li>No sample-specific properties such as concentration or composition are required to assess size, size distributions and aggregation.</li> <li>Temperature is controlled and continuously measured in the instrument</li> </ul> <p>For advanced measurements, a few more parameters need to be selected or entered into DYNAMICS:</p> <ul> <li>Particle concentration: input sample refractive index</li> <li><i>k</i><sub>D</sub> and <i>A</i><sub>2</sub>: input sample concentration</li> </ul> <p>The <a href="/library/theory/dynamic-light-scattering-theory.html">DLS Theory page</a> contains additional details on the technique, including a video of Brownian motion.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2019/06/DLS-Icon-Blue-Orange-500.jpg" width="80%"></p> <p align="center"> <img alt="dls" src="/wp-content/uploads/2021/10/dls-theory-equation-1.jpg" width="60%"></p> <p align="left" style="color:#075c97;font-size:14px;padding-left:20px">The Stokes- Einstein relationship describes how the hydrodynamic radius <i>R</i><sub>h</sub> is calculated from the diffusion coefficient <i>D</i><sub>t</sub>. <i>T</i> is the temperature (in Kelvin), <i>k</i> is the Boltzman constant and <i><font face="Symbol">h</font></i> is the viscosity of the solution. </p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>What are available DLS options and configurations?</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4 style="color:#075c97;"><b>Measure directly in microwell plates</b></h4> <p>High-throughput, automated measurements performed directly in standard 96-, 384- or 1536-well microwell plates reveal new horizons for dynamic light scattering. Some of the high-throughput screening tasks accomplished with the DynaPro Plate Reader include:</p> <ul> <li>Quantifying the type and degree of aggregation in hundreds of buffer conditions, important in formulation and crystallization studies</li> <li>Evaluating the developability of pre-formulations of biotherapeutics</li> <li>Formulation development, e.g., assessment of buffer excipients and pH for colloidal stability using the diffusion interaction parameter <i>k</i><sub>D</sub></li> <li>Assessing multiple formulations for stability, e.g., freeze-thaw cycles, accelerated aging studies or thermal stability screening using the aggregation temperature <i>T</i><sub>agg</sub> or melting temperature <i>T</i><sub>m</sub></li> <li>Determining the critical aggregation concentration for small molecules such as compound aggregates</li> <li>Integration of plate-based testing protocols with other assays in well-plate format</li> </ul> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2021/10/Welll-Plate-Sizes.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4 style="color:#075c97;"><b>Conventional cuvette-based systems</b></h4> <p>For easy and convenient measurement of a few samples, all you need is a few microliters of your sample, a disposable or quartz cuvette and a dynamic light scattering instrument such as the <a href="/products/instruments/dynapro-nanostar-dynamic-light-scattering-detector.html">DynaPro<sup>™</sup> NanoStar<sup>™</sup> </a> or <a href="/products/instruments/zetastar.html">DynaPro<sup>™</sup> ZetaStar<sup>™</sup> </a> with DYNAMICS software for data acquisition and analysis.</p> <ul> <li>NanoStar has a 90° DLS detector to measure size and size distributions in the range of 0.2 to 1000 nm radius and requires sample volumes as low as 2 µL. In addition, the dedicated 90° SLS detector enables measurement of particle concentration, non-specific interactions, turbidity and molecular weight.</li> <li>Mobius has a 163.5° DLS detector to measure size and size distributions up to 2500 nm radius in the flow cell and requires 170 µL of sample. With the quartz cuvette, sample volumes as low as 45 µL may be measured.</li> <li>ZetaStar has a 163.5° and 90° DLS detector to measure size and size distributions in the range of 0.2 to 1000 nm radius and requires sample volumes as low as 2 uL. In addition, the dedicated 90 SLS detector enables measurement of particle concentration, non-specific interactions, turbidity, and molecular weight. The PEEK flow cell and disposable cell can both measure DLS simultaneously with ELS data using the back-scatter angle as well.</li> </ul> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2023/11/Cuvettes.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4 style="color:#075c97;"><b>Integration with SEC-MALS</b></h4> <p>On-Line DLS is a valuable addition to your SEC-MALS or FFF-MALS systems, providing hydrodynamic size and, in combination with MALS, information about conformation, elution behavior and the shape of larger nanoparticles. Instead of adding another detector in-line, with the associated band broadening, dilution, signal misalignment and loss of resolution, Wyatt's innovative solution is to share the MALS laser and flow cell with the DLS detector.</p> <p>A <a href="https://store.wyatt.com/shop/udawn/udawn3/wiq/">WyattQELS<sup>™</sup></a> DLS module may be embedded within a MALS instrument, or a NanoStar or ZetaStar can be coupled to a MALS detector employing an optical fiber. <a href="/products/software/astra.html">ASTRA<sup>™</sup> </a> chromatography software connects to both instruments to acquire data simultaneously, analyzing and reporting the individual information on size (rms radius by MALS and hydrodynamic radius by DLS) and molecular weight to give insights on molecular conformation and chromatography.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt=NanoStar DAWN" src="/wp-content/uploads/2022/01/19_NanoStar_Dawn_Hero.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4 style="color:#075c97;"><b>Robotic interface</b></h4> <p>Advanced lab automation is available via the DYNAMICS Automation API. Using the DYNAMICS Automation API to operate DYNAMICS remotely, the DynaPro Plate Reader may be integrated within a robotic workflow. This feature is also available for ZetaStar or NanoStar for an automated workflow or experimental apparatus.</p> <h4 style="color:#075c97;"><b>Measurement automation</b></h4> <p>DYNAMICS and HPLC Connect software pair with the ZetaStar to enable automated sample handling with a Waters Autosampler. The autosampler and HPLC pump are controlled directly by DYNAMICS to flush the system, inject sample, perform the measurement, and repeat with the next sample. The system may even be programmed to automatically prepare samples with a series of buffer conditions. Autosampler and HPLC pump are controlled directly by DYNAMICS to flush the system, inject sample, perform the measurement, and repeat with the next sample. The system may even be programmed to automatically prepare samples with a series of buffer conditions.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:30px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dynamics" src="/wp-content/uploads/2023/02/DYNAMICS-Automation-API-Diagram.jpg" width="70%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> </div></div> </div><div id="dls-3" ><h2 style="color:#075c97;">Key DLS applications</h2> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>DLS is a key characterization technique for sizing, assessing aggregations and measuring stability in nanomedicines, proteins and related bio-macromolecules as well as nanoparticles. We’ve listed some representative applications with application notes and on-demand webinar resources below. Our searchable <a href="/library/bibliography.html">Bibliography</a>, containing thousands of references to peer-reviewed articles citing Wyatt instruments, is an excellent resource for researching your specific DLS applications.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2021/10/Sophia-LSU-Lab-800.jpg" width="80%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="accordion"><div class="mfn-acc accordion_wrapper "> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Proteins and bio-macromolecules</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4>Formulations and aggregation</h4> <p>Assessing a drug candidate’s suitability early in the discovery and development stages is essential for minimizing the risk of costly downstream failure. DLS delivers essential insight into biopharmaceutical candidates and formulations through pre-formulation studies, early and late-stage formulations as well as quality control.</p> <p><i>Explore the versatility of dynamic light scattering in our white paper <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/white-papers/WP5010-HT-DLS-for-developability-and-pre-formulation-of-biotherapeutics.pdf" target="_blank">WP5010: Screening Developability and Pre-Formulation of Biotherapeutics with High-Throughput Dynamic Light Scattering (HT–DLS)</a>, and our publication list <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/others/PL5011-HT-formulation-of-biopharmaceuticals.pdf" target="_blank">PL5011: Key publications on high-throughput screening of therapeutic protein formulations by light scattering</a>.</i></p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2021/10/dls-solutions-app-1.jpg"></p> <p align="left" style="color:#075c97;font-size:14px;padding-left:15px">The heat map display in DYNAMICS™ may be programmed to differentiate size distributions and visualize at a glance different degrees of aggregation. Data courtesy Sabin Vaccine Institute.</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4>Colloidal and thermal stability</h4> <p>The rational biophysical characterization of therapeutic protein candidates and their formulations can greatly improve the early-stage development process of new biologics. DLS is crucial to understanding the thermal and long-term stability of therapeutically relevant proteins in different formulation conditions.</p> <p><i>View the webinars <a href="/library/webinars/rational-use-of-dynamic-light-scattering-for-the-characterization-of-cytokines-bispecific-antibody-fusion-proteins-and-binary-antibody-mixtures.html">Rational use of dynamic light scattering for the characterization of cytokines, bispecific antibody fusion proteins and binary antibody mixtures</a> and <a href="/library/webinars/predicting-stability-of-antibody-therapeutics-by-unfolded-state-studies-with-high-throughput-dynamic-light-scattering-ht-dls.html">Predicting stability of antibody therapeutics by unfolded-state studies with high-throughput dynamic light scattering (HT-DLS)</a> for more information.</i></p> <br><br> <h4>Antibody-drug conjugates</h4> <p>Antibody-drug conjugates (ADCs) are important biotherapeutic candidates that combine highly potent cytotoxic drugs with monoclonal antibodies (mAb) for targeted drug delivery in the treatment of cancer or neurodegenerative disorders. </p> <p><i>Read the application note <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/AN5005-characterization-and-formulation-screening-of-mAb-and-ADCs-by-high-throughput-DLS.pdf" target="_blank">AN5005: Characterization and Formulation Screening of mAb and Antibody-Drug Conjugates (ADCs) by High-throughput DLS</a> to learn how the DynaPro Plate Reader is an effective tool for rapid formulation screening</i>.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:25px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2021/10/dls-solutions-app-2.jpg"></p> <p align="left" style="color:#075c97;font-size:14px;padding-left:20px">The diffusion interaction parameter, <i>k</i><sub>D</sub> , decreases prior to the point of aggregation as protein molecules begin to unfold. The onset of aggregation is measured by SLS. Blue diamonds: <i>k</i><sub>D</sub> values; black line: <i>R</i><sub>h</sub> values; red circles: SLS values. Three distinct transition temperatures are evident. The <i>k</i><sub>D</sub> scale has been inverted for clarity</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Nanomedicines</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4>Small viral vectors and virus-like particles</h4> <p>Viruses like AAV, adenovirus and lentivirus, as well as virus-like particles may be characterized by DLS for critical quality attributes such as size, aggregate content, thermal stability and total viral particle concentration.</p> <p><i>To learn more, view <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/AN5007-AAV-quantitation-and-stability-analysis-by-batch-DLS.pdf" target="_blank">AN5007: Characterization of AAV-based viral vectors by DynaPro DLS/SLS instruments</a>.</i></p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2021/10/AAV-1000.jpg" width="70%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4>Liposomes</h4> <p>Liposomes are one of the most frequently used nanocarriers to encapsulate small molecule drugs. FFF-MALS-DLS is a powerful tool for characterizing the size, particle concentration and structure of large nanoparticles to distinguish between filled and empty liposomes.</p> <p><i>View <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/fff-mals/AN2601-liposome-structure-fff-qels.pdf" target="_blank">AN2601: Liposome Size, Concentration and Structural Characterization by FFF-MALS-DLS</a>.</i></p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"><a rel="lightbox" href="/wp-content/uploads/2021/10/WP2605-empty-full-liposomes.png"><img alt="sec-mals" src="/wp-content/uploads/2021/10/WP2605-empty-full-liposomes.png"></a></p> <p align="left" style="color:#075c97;font-size:14px;padding-left:15px">Hydrodynamic radius (a) and root-mean square radius (b) plotted against FFF elution time, overlaid with 90° LS signals for empty (red) and filled (green) liposomes. The <i>R</i><sub>h</sub> and <i>R</i><sub>g</sub> values are determined by the respective online DLS and MALS detectors</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4>Lipid Nanoparticles</h4> <p>Lipid nanoparticles are of increasing importance for small molecule drug delivery, and more recently, nucleic acid delivery. Characterization needs include determination of size, titer, stability and aggregation behavior.</p> <p><i>Learn how DLS, SEC-MALS and FFF-MALS can be used to characterize this important class of nanomedicines in our recent webinar <a href="/library/webinars/size-concentration-payload-and-quality-comprehensive-characterization-of-lnp-rna-therapeutics-by-light-scattering.html">Size, Concentration, Payload and Quality: Comprehensive Characterization of LNP-RNA Therapeutics by Light Scattering</a>.</i></p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2021/10/LNP-1000.jpg" width="70%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4>Nanocapsules</h4> <p>Protein-based nanocapsules are highly interesting for stimuli-responsive targeted drug delivery, as protein properties can directly influence particle stability and release behavior.</p> <p><i>Learn how properties of nanodispersions, such as dispersion stability, agglomeration and temperature stability based on particle size analysis can be explored in <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-cuvette/AN4001-organic-phase-encapsulation-by-protein-particles-DLS.pdf" target="_blank">AN4001: Organic-phase encapsulation in protein-based nanocapsules studied by DLS</a>.</i></p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2021/10/Nanoparticle-1000.jpg" width="70%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Colloids and Nanoparticles</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4>Environmental nanoparticles</h4> <p>Characterization of nanoparticle size and distribution in biological environments and understanding the parameters that affect them are imperative to accurately assessing nanoparticle toxicity. Delivering nanoparticles in a well-dispersed form is necessary for accurate assessment of their toxicity.</p> <p><i>Read <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/AN5001-TiO2-nanoparticle-dispersions-in-cell-culture-media-by-HT-DLS.pdf" target="_blank">AN5001: TiO2 nanoparticle dispersions in cell culture media characterized by high-throughput DLS</a> to learn more.</i></p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2021/10/dls-solutions-app-5.jpg"></p> <p align="left" style="color:#075c97;font-size:14px;padding-left:10px">SpectralView display of TiO<sub>2</sub> nanoparticle dispersion in water and six different cell culture media (BEGM, DMEM, LB, TSB, SD, and YPD) suggests severe agglomeration in all media without dispersing agents, improved dispersion in most media using 2 mg/mL BSA, and the best dispersion using ≥1 wt.% FBS.</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <h4>Drug nanosuspensions</h4> <p>Drug nanosuspensions are a promising approach to formulation and consist of crystalline drug particles that are generally <400 nm in diameter suspended in aqueous solution. Nanocrystalline particles have a large surface area relative to their overall sizes, which results in a dramatically increased rate of dissolution, improved absorption rates and increased bioavailability.</p> <p><i>Explore <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/AN5002-High-Throughput_Tools_for_Optimizing_Drug_Nanosuspensions.pdf" target="_blank">AN5002: High-Throughput Tools for Optimizing Drug Nanosuspensions</a> to learn how the DynaPro Plate Reader is key in optimizing these formulations in early drug discovery.</i></p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:35px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2021/10/an5002.png"></p> <p align="left" style="color:#075c97;font-size:14px;padding-left:10px">Acoustic resonance milling effectively reduces solid compound to sub-micron particles, rapidly and in a small volume.</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> </div></div> </div><div id="dls-4" ><h2 style="color:#075c97;">Dynamic light scattering – measuring with ease</h2> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>DLS an essential characterization tool for proteins, nanomedicines and other nanoparticles to screen for properties such as size, aggregation, stability and interactions. The technique’s versatility and simplicity has contributed significantly to its popularity.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2021/10/dls-solutions-workflow-1-updated.png"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="accordion"><div class="mfn-acc accordion_wrapper "> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>How do I use DLS?</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Running a DLS experiment is straightforward. A typical DLS experiment comprises the following steps:</p> <ol type="1"> <li>Sample preparation (minimal, may include filtration or dilution)</li> <li>Load sample into a cuvette or an array of samples into a microwell plate</li> <li>Place the cuvette or microwell plate into your instrument</li> <li>Collect data manually or using automation (see below)</li> <li>Analyze data</li> </ol> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2022/01/Pipetting-Sample.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p><b>Data collection may be automated in several ways:</b></p> <ul> <li>Fast screening: Experiment Designer - Perform full or partial plate scans, thermal or time stability studies by simply selecting a range of wells or single wells for measurements with a mouse-click</li> <li>Maximum flexibility: Event Scheduler - Design any standard operating procedure, be it a time or thermal sequence, autosampler automation, exploration of experimental parameters or any combination thereof.</li> <li>Fully automatic: Robotic integration - Use DYNAMICS API to interface with a liquid handling robotic system to automatically load or unload wellplates and run, process, save and export experiments.</li> </ul> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:40px 30px 0px 30px;line-height:125%"> <p align="center"> <a href="/wp-content/uploads/2021/10/dls-solutions-workflow-2.png" rel="lightbox"><img alt="dls" src="/wp-content/uploads/2021/10/dls-solutions-workflow-2.png"></a></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Sample and instrument preparation</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Instrument setup is minimal: Just switch on your instrument and let it equilibrate to the correct temperature. Some precautions must be taken when preparing samples as light scattering is sensitive to particulates. A few simple steps will ensure successful analysis:</p> <ul> <li>Wyatt disposable cuvettes are individually packed in a clean room and should remain wrapped until use. Quartz cuvettes should be capped when stored.</li> <li>Buffers should be filtered to 0.02 µm or 0.1 µm. Measuring pure buffer is recommended as a control.</li> <li>Samples should be centrifuged or filtered in a way that does not remove any analytes of interest.</li> </ul> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2021/10/Quartz-Cuvette.jpg" width="70%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>DYNAMICS</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Data collection is straightforward: Click the Start button to run single measurements or use the experiment designer or event scheduler described above to collect data automatically. All results are acquired in real-time and analyzed automatically. View your results in a data table or graphically. All original data are accessible and may be reprocessed at any time. Some of the analysis features are:</p> <ul> <li>Size, size distributions and molar mass</li> <li>Zeta potential and charge</li> <li>Particle concentration of biopharmaceuticals</li> <li>Thermal stability such as <i>T</i><sub>onset</sub>, <i>T</i><sub>agg</sub> and <i>T</i><sub>m</sub>, as well as average size at transition points</li> <li>Formulation colloidal stability via the diffusion interaction parameter <i>k</i><sub>D</sub> and second virial coefficient <i>A</i><sub>2</sub></li> <li>Solution viscosity</li> <li>General parametric analysis</li> </ul> <p>For those in GMP/GLP-regulated environments, DYNAMICS’s Security Pack add-on enables 21 CFR Part 11 compliance including full audit trails and electronic signatures.</p> <p>Wyatt Technology provides several avenues for supporting novice and advanced users, including a full set of tutorials, training materials and technical notes in the online <a href="https://wyatttechnology.zendesk.com/hc/en-us">Wyatt Support Center</a>, phone support and <a href="/training/light-scattering-university.html">Light Scattering University</a>. Site visits may be arranged for IQ/OQ, service and preventive maintenance as well as group training.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2021/10/dls-workflow-solutions-3-updated.jpg"></p> <p align="left" style="color:#075c97;font-size:14px">Melting/aggregation curve data and analyses for four proteins measured simultaneously, in triplicate, on the DynaPro Plate Reader.</p> <br><br> <p align="center"> <img alt="21 cfr part 11 compliance" src="/wp-content/uploads/2021/06/FDA-Compliant-Stamp-Gray-600.jpg" width="70%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>DYNAMICS Touch</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p><a href="/products/software/dynamics-touch.html">DYNAMICS<sup>™</sup> Touch<sup>™</sup></a> is the on-board application for quick and easy walk-up measurements of size and molar mass on the <a href="/products/instruments/dynapro-nanostar-dynamic-light-scattering-detector.html">DynaPro<sup>™</sup> NanoStar<sup>™</sup></a> and <a href="/products/instruments/zetastar.html">DynaPro<sup>™</sup> ZetaStar<sup>™</sup></a>.</p> <p>The intuitive touch-screen interface guides you through each step of your measurement. Load the cuvette with your sample into the instrument and:</p> <p><b>1. Choose what you wish to measure</b> <ul> <li> Size (hydrodynamic radius by <a href="/library/theory/dynamic-light-scattering-theory.html">DLS</a>)</li> <li> Molar mass (by <a href="/library/theory/multi-angle-light-scattering-theory.html">SLS</a> and DLS)</li> </ul> <p><b>2. Input parameters and collect data</b></p> <p>Step by step, specify the:</p> <ul> <li>Cuvette (quartz, disposable, or generic)</li> <li>Sample (<i>dn/dc</i>, concentration, <i>A</i><sub>2</sub>)</li> <li>Solvent (predefined or custom)</li> <li>Temperature</li> <li>Number of replicates</li> <li>Next, simply press ‘play’ to start the measurement. </li> </ul> <p>You can also manage commonly used cuvettes, custom solvents, and other key parameters directly in DYNAMICS Touch and use presets to streamline your workflow and ensure all users are performing the same analyses.</p> <p><b>3. Examine and share your results</b></p> <p>All results are analyzed automatically and presented to the user directly in DYNAMICS Touch at the end of each measurement. The automatic data quality assessment allows even first-time users to understand their results and identify any issues with their samples.</p> <p>The resulting experiment data files and reports can be downloaded to a computer and shared or further analyzed in <a href="/products/software/dynamics.html">DYNAMICS<sup>™</sup></a>. The PDF report may even be viewed immediately on your mobile device, network security permitting.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <a href="/wp-content/uploads/2022/01/Dashboard-Page-Conc.jpg" rel="lightbox"><img alt="dls" src="/wp-content/uploads/2022/01/Dashboard-Page-Conc.jpg"></a></p> <br><br> <p align="center"><a href="/wp-content/uploads/2021/10/dynamics-touch-image3.png" rel="lightbox"><img alt="dls" src="/wp-content/uploads/2021/10/dynamics-touch-image3.png"></a></p> <br><br> <p align="center"> <a href="/wp-content/uploads/2021/10/My-Sample-UI-Lock-9-28-21.jpg" rel="lightbox"><img alt="dls" src="/wp-content/uploads/2021/10/My-Sample-UI-Lock-9-28-21.jpg"></a></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> </div></div> </div><div id="dls-5" ><h2 style="color:#075c97;">Unique instrumentation for demanding research</h2> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Developed and built entirely in-house at Wyatt’s Santa Barbara headquarters, all of these products incorporate state-of-the-art technology combined with rigorous quality assurance practices. Their rich touch screen displays ensure access to all the control functions, data and diagnostics needed for smooth operation and rapid troubleshooting of samples or instrumentation, should the need arise.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="sec-mals" src="/wp-content/uploads/2023/09/ZetaStar-Over-Shoulder-1000.jpg" width="90%"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="accordion"><div class="mfn-acc accordion_wrapper "> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Dynamic Light Scattering/Electrophoretic Light Scattering Instruments</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p><b><a href="/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html">DynaPro<sup>™</sup> Plate Reader</b></a> - The only commercially available instrument that measures DLS and SLS for size molar mass and peak-resolved particle concentration, directly in situ in standard 96, 384 or 1536 microwell plates. Measures second virial coefficient <i>A</i><sub>2</sub> and diffusion interaction parameter <i>k</i><sub>D</sub>. The non-perturbing DynaPro Plate Reader offers integration with robot liquid handlers and multi-technique plate-based assay protocols. Temperature-controlled from 4 °C to 85 °C. An integrated camera views each well to observe precipitation or turbidity as well as troubleshooting and diagnostics.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2021/08/DynaPro-Plate-Reader-III-1000.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p><a href="/products/instruments/zetastar.html"><b>DynaPro<sup>™</sup> ZetaStar<sup>™</sup></b></a> - A manual or automated light scattering instrument for analysis of size distribution, particle concentration, molecular weight, turbidity and zeta potential of proteins, nanomedicines, gene vectors, extracellular vesicles, nanoemulsions and polymers. It uniquely measures DLS and ELS simultaneously and can measure zeta potential in salts solutions greater than physiological conditions.</p> <p><b><a href="https://store.wyatt.com/shop/udawn/udawn3/wiq/">WyattQELS<sup>™</sup></b></a> - An online dynamic light scattering module that may be embedded into any MALS instrument to provide integrated and simultaneous DLS data.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2023/09/DynaPro-ZetaStar-Hero-View.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p><b><a href="/products/instruments/dynapro-nanostar-dynamic-light-scattering-detector.html">DynaPro<sup>™</sup> NanoStar<sup>™</sup></b></a> - With sample volumes as small as 2 µL and temperature control spanning -10 °C to +120 °C, NanoStar goes above and beyond traditional cuvette-based DLS instruments. It offers an optimized static light scattering detector in parallel to the DLS detection system to determine absolute molar mass, <a href="/solutions/measuring-turbidity-opalescence.html">turbidity</a> and in conjunction with DLS, particle concentration per peak.</p> <p>The intuitive <a href="/products/software/dynamics-touch.html">DYNAMICS<sup>™</sup> Touch<sup>™</sup></a> on-board app, running on the NanoStar’s generous touch screen, enables walk-up operation and basic measurements even by DLS novices. Extended and more comprehensive analyses are carried out in DYNAMICS, running on a standard PC.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls, dynapro nanostar, dynamic light scattering instrument" src="/wp-content/uploads/2022/01/NanoStar_Hero_Display.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Software</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p><b><a href="/products/software/dynamics.html">DYNAMICS<sup>™</sup></b></a> - Versatile software for size, size distributions, particle concentration, zeta potential and charge measurements, combining flexibility and intelligence with rigorous calculations and advanced analysis. DYNAMICS offers an optional <a href="/products/services/21-cfr-part-11-compliance.html">21 CFR Part 11 compliant</a> security and database package for GMP and GLP laboratories.</p> <p><b><a href="/products/software/dynamics-touch.html ">DYNAMICS Touch</a></b> – measure and analyze DLS, ELS, and SLS data directly on the ZetaStar touch screen with the on-board app.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:10px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2020/08/DYNAMICS-Splash-Screen-Web504-200.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p><b><a href="/products/software/astra.html">ASTRA<sup>™</sup></b></a> - Our comprehensive software solution for MALS and DLS analysis in chromatography of field-flow fractionation. ASTRA is available in a 21 CFR Part 11 compliant version and offers additional options and advanced analyses.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:10px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="dls" src="/wp-content/uploads/2020/08/ASTRA-Splash-Screen-Web-504-200.jpg"></p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> </div></div> </div><div id="dls-6" ><iframe src="https://player.vimeo.com/video/864609160" width="640" height="360" frameborder="0" allow="autoplay; fullscreen" allowfullscreen></iframe> <p><b>Introducing the DynaPro<sup>™</sup> ZetaStar<sup>™</sup></b></p> <iframe src="https://player.vimeo.com/video/673234624" width="640" height="360" frameborder="0" webkitallowfullscreen="" mozallowfullscreen="" allowfullscreen=""></iframe> <p><b>Introducing the DynaPro<sup>™</sup> NanoStar<sup>™</sup> II</b></p> <iframe src="https://player.vimeo.com/video/239201761" width="640" height="360" frameborder="0" webkitallowfullscreen="" mozallowfullscreen="" allowfullscreen=""></iframe> <p><b>Introducing the DynaPro Plate Reader III</b></p> <br> <iframe src="https://player.vimeo.com/video/239201865" width="640" height="360" frameborder="0" webkitallowfullscreen="" mozallowfullscreen="" allowfullscreen=""></iframe> <p><b>Step up to automated DLS</b></p> </div><div id="dls-7" ><h2 style="color:#075c97;">Explore DLS</h2> <p>Below you will find a partial list of the resources available on our website where you can learn more about DLS and how it is used by scientists around the world, in academia, industry and government labs for advanced macromolecular and nanoparticle characterization. </p> <div class="accordion"><div class="mfn-acc accordion_wrapper "> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Webinars</h3></div><div class="answer"> <p>On-demand webinars presenting the theory and applications of DLS are available for unrestricted viewing on the <a href="/library/webinars/dls.html">DLS Webinars page</a>. Listed below are some of the most popular:</p> <h4><b>Biotherapeutics</b></h4> <ul> <li><a href="/library/webinars/virtual-user-meeting-december-2020.html">Virtual User Meeting – Screening for stability: Characterizing formulations with DLS</a></li> <li><a href="/library/webinars/rational-use-of-dynamic-light-scattering-for-the-characterization-of-cytokines-bispecific-antibody-fusion-proteins-and-binary-antibody-mixtures.html">Rational use of dynamic light scattering for the characterization of cytokines, bispecific antibody fusion proteins and binary antibody mixtures</a></li> <li><a href="/library/webinars/understanding-monoclonal-antibody-unfolding-and-aggregation.html">Understanding Monoclonal Antibody Unfolding and Aggregation</a></li> <li><a href="/library/webinars/high-throughput-analytics-for-formulation-and-process-development-of-bio-and-nano-therapeutics.html">High-throughput Analytics for Formulation and Process Development of Bio- and Nano-therapeutics</a></li> </ul> <h4><b>Nanoparticles</b></h4> <ul> <li><a href="/library/webinars/size-concentration-payload-and-quality-comprehensive-characterization-of-lnp-rna-therapeutics-by-light-scattering.html">Size, Concentration, Payload and Quality: Comprehensive Characterization of LNP-RNA Therapeutics by Light Scattering</a></li> <li><a href="/library/webinars/investigating-hydrogel-microparticles-with-light-scattering-and-microscopy-tools.html">Investigating Hydrogel Microparticles with Light Scattering and Microscopy Tools</a></li> <li><a href="/library/webinars/virtual-user-meeting-june-2020.html">Virtual User Meeting – Viral Vectors and Bio-Nanoparticles</a></li> <li><a href="/library/webinars/novel-ways-of-screening-colloidal-nanoparticles-under-preclinical-relevant-conditions.html">Novel Ways of Screening Colloidal Nanoparticles Under Preclinical-relevant Conditions</a></li> </ul> <h4><b>Proteins</b></h4> <ul> <li><a href="/library/webinars/the-versatility-of-high-throughput-dynamic-light-scattering-in-protein-characterization-and-formulation-development.html">The Versatility of High-Throughput Dynamic Light Scattering in Protein Characterization and Formulation Development</a></li> <li><a href="/library/webinars/predicting-and-evaluating-the-stability-of-protein-based-drugs-and-formulations-by-dynamic-light-scattering-and-machine-learning.html">Predicting and Evaluating the Stability of Therapeutic Protein Formulations by Dynamic Light Scattering and Machine Learning</a></li> </ul> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Application Notes</h3></div><div class="answer"> <p>Application notes highlighting the use of DLS are available for unrestricted viewing on the <a href="/library/application-notes/by-technique/dls.html">DLS Application Notes page</a>. Listed below are some of the most popular:</p> <h4><b>Biotherapeutics</b></h4> <ul> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/white-papers/WP5010-HT-DLS-for-developability-and-pre-formulation-of-biotherapeutics.pdf" target="_blank">WP5010: Screening Developability and Pre-Formulation of Biotherapeutics with HT-DLS</a></li> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/AN5005-characterization-and-formulation-screening-of-mAb-and-ADCs-by-high-throughput-DLS.pdf" target="_blank">AN5005: Characterization and Formulation Screening of mAb and Antibody-Drug Conjugates (ADCs) by High-throughput DLS</a></li> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/others/PL5011-HT-formulation-of-biopharmaceuticals.pdf">PL5011: Key publications on high-throughput screening of therapeutic protein formulations by light scattering</a></li> </ul> <h4><b>Nanoparticles</b></h4> <ul> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/AN5001-TiO2-nanoparticle-dispersions-in-cell-culture-media-by-HT-DLS.pdf" target="_blank">AN5001: TiO2 nanoparticle dispersions in cell culture media</a></li> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/AN5002-High-Throughput_Tools_for_Optimizing_Drug_Nanosuspensions.pdf" target="_blank">AN5002: High-Throughput Tools for Optimizing Drug Nanosuspensions</a></li> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/white-papers/Nanoparticle-Characterization-with-the-Light-Scattering-Toolkit.pdf" target="_blank">WP9001: Overcoming the Challenges of Nanoparticle Characterization with a Light Scattering Toolkit</a></li> </ul> <h4><b>Proteins</b></h4> <ul> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/AN5006-Probing-Aggregation-Rate-of-a-Tau-Protein.pdf" target="_blank">AN5006: Probing the aggregation rate of Tau protein with automated DLS and SLS</a></li> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-plate/WP5003-Automated-dynamic-and-static-light-scattering-in-microwell-plates.pdf" target="_blank">WP5003: Automated dynamic and static light scattering in microwell plates for fast, productive development of biologics</a></li> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/dls-cuvette/AN4001-organic-phase-encapsulation-by-protein-particles-DLS.pdf" target="_blank">AN4001: Organic-phase encapsulation in protein-based nanocapsules studied by DLS</a></li> <li><a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-proteins/AN7301-glycoprotein-SEC-MALS-QELS.pdff" target="_blank">AN7301: Glycoprotein by SEC-MALS-QELS</a></li> </ul> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Theory</h3></div><div class="answer"> <p>Please see the following page for details on DLS theory: <a href="">Understanding Dynamic Light Scattering Theory</a>.</p> <p>These pages provide additional information on specific analyses performed in DLS:</p> <ul> <li><a href="/solutions/properties/nanoparticle-and-macromolecular-size-by-light-scattering.html">Size</a></li> <li><a href="/solutions/properties/particle-concentration.html">Particle concentration</a></li> </ul> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Bibliography</h3></div><div class="answer"> <p>An extensive searchable bibliography of publications citing Wyatt DLS instruments is available at <a href="/library/bibliography.html">www.wyatt.com/Bibliography</a>. Just open the Advanced Search and click the Dynamic Light Scattering box, then enter your search terms below.</p> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Technical Notes</h3></div><div class="answer"> <p>Wyatt offers its customers comprehensive online support via the <a href="https://wyatttechnology.zendesk.com/hc/en-us/">Wyatt Support Center</a>, including many technical notes that can help make the most of DLS experiments. If you are a customer and do not have access, please request an account on the Support Log-in page.</p> </div></div> </div></div> </div><div id="dls-8" ><h2 style="color:#075c97;">At Wyatt, it’s personal</h2> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Your success is ours! From the time you purchase a Wyatt instrument, we are there for you every step of the way and give you more than just an instrument warranty. Wyatt provides a full suite of support offerings to help you make the most of your investment. Most importantly, you get unrivaled personal attention to ensure success and productivity.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"> <p align="center"> <img alt="fff-mals" src="/wp-content/uploads/2020/10/Lab-Users-300.jpg" width="60%"> </p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="accordion"><div class="mfn-acc accordion_wrapper "> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>On-site installation and training</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>With every instrument purchase, we offer an on-site installation and familiarization visit at your laboratory to ensure proper instrument set-up and function. We also offer customized on-site training tailored to your specific needs. These additional services may be especially desirable if a large group of staff members wants to receive instruction. </p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"><p align="center" style="color:#075c97;font-size:14px"><img alt="fff-mals" src="/wp-content/uploads/2020/10/Camille-Lab-Class-600.jpg" width="90%"> </p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>First-year service and support</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>All new Wyatt instruments come with a full year of unlimited telephone and e-mail support as well as our standard full-coverage warranty during the first year. Our congenial staff of Ph.D. scientists and expert technicians provides both technical and application assistance. We will show you how to analyze your data or set up instrument communications by remotely accessing your PC.</p> <p>After the first year, annual service plans are available for continuing, unlimited telephone support, e-mail support and instrument service. Benefits of the service plans include annual preventive maintenance, on-site calibration, re-qualification, and discounts on parts and labor for repairs. <a href="/products/services/service-contracts.html">Learn more about service plans.</a></p> <p>The specifics of first-year service as well as continuing support may vary by region. Please contact <a href="/contact/global-offices.html">your local Wyatt representative</a> for more information.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"><p align="center" style="color:#075c97;font-size:14px"><img alt="fff-mals" src="/wp-content/uploads/2020/10/Customer-Service-600.jpg" width="90%"> </p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>IQ/OQ and compliance</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Wyatt Technology offers a complete compliance program including documentation and on-site validation for all of its instruments. We provide the resources and tools necessary to ensure compliance. We’ve worked extensively with our partners in compliant environments to build up a robust set of documents and services. Wyatt instruments and software are used in GMP environments at pharmaceutical, biotech, and other regulated industries around the world. <a href="/products/services/iqoq-validation.html">Learn more about our Compliance program.</a></p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"><p align="center" style="color:#075c97;font-size:14px"><img alt="fff-mals" src="/wp-content/uploads/2019/07/IQ-OQ-Service.jpg"> </p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Light Scattering University</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Our flagship training program Light Scattering University<sup>®</sup> (LSU) is included with every purchase of a light scattering instrument. In the 2, 3 or 4 day course—depending on which system is purchased—LSU students discover advanced data processing methods and alternative analytical tools they may not be familiar with. The students also learn how their MALS and DLS data complements information from other techniques they are using in the lab. Advanced classes are offered for LSU graduates on more detailed techniques and topics. </p> <p>During LSU our students meet Dr. Philip Wyatt, Founder and Chairman of the Board who gives an acclaimed Light Scattering Instrument Museum tour. Students also meet those who invent, build, service and support the instruments, so they know the team that will support them when back in the lab.</p> <p>An LSU credit, inclusive of airfare, accommodations and most meals, is included for North American customers who purchase one of our light scattering instruments. While you are here, we work you hard but feed you well—at a variety of Santa Barbara restaurants!</p> <p>Learn more <a href="/training/light-scattering-university.html">here</a>.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px;line-height:125%"><p align="center" style="color:#075c97;font-size:14px"><img alt="fff-mals" src="/wp-content/uploads/2019/10/1-Michelle-Chen-Teaching-500.jpg" width="90%"> </p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title "><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Service and Support Plans</h3></div><div class="answer"> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>At Wyatt, we understand that downtime and loss of productivity is critical and have developed comprehensive service offerings — our Platinum, Gold and Silver Service Plans — specifically designed to make preventative maintenance and instrument repair requests quick, smooth and hassle-free. These plans are available on an annual basis after the first-year instrument warranty expires and offer priority phone and e-mail support in addition to instrument service.</p> <p>The Platinum and Gold Service Plans offer comprehensive on-site preventive maintenance, repair services and software updates. A highly trained Wyatt Field Service Engineer will come to your facility and perform annual or semi-annual preventative maintenance, calibration and optional re-qualification checks. We also offer loaner units should an instrument require factory repair. 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