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ul.mfn-megamenu-bg{padding-left:166px;padding-right:20px;background-position:left bottom}#Top_bar .menu > li > ul.mfn-megamenu-bg > li{background:none}#Top_bar .menu > li > ul.mfn-megamenu-bg > li a{border:none}#Top_bar .menu > li > ul.mfn-megamenu-bg > li > ul{background:none!important;-webkit-box-shadow:0 0 0 0;-moz-box-shadow:0 0 0 0;box-shadow:0 0 0 0}.mm-vertical #Top_bar .container{position:relative}.mm-vertical #Top_bar .top_bar_left{position:static}.mm-vertical #Top_bar .menu > li ul{box-shadow:0 0 0 0 transparent!important;background-image:none}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu{padding:20px 0}.mm-vertical.header-plain #Top_bar .menu > li > ul.mfn-megamenu{width:100%!important;margin:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li{display:table-cell;float:none!important;width:10%;padding:0 15px;border-right:1px solid rgba(0,0,0,0.05)}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}body.header-shop #Top_bar #menu{display:flex!important;background-color:transparent}.header-shop #Top_bar.is-sticky .top_bar_row_second{display:none}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;transform:unset}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:not(.menu-toggle):after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span{border-width:0!important}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}.menuo-no-borders.header-plain #Top_bar a#header_cart,.menuo-no-borders.header-plain #Top_bar a#search_button,.menuo-no-borders.header-plain #Top_bar 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#Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background-color:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar #menu{background-color:transparent}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{margin:0;width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}.menuo-arrows #Top_bar .menu > li.submenu > a > span:after{content:unset!important}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:left;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{content:none}#Header_creative #Top_bar .top_bar_right .top_bar_right_wrapper{flex-wrap:wrap;justify-content:center}#Header_creative #Top_bar .top_bar_right .top-bar-right-icon,#Header_creative #Top_bar .top_bar_right .wpml-languages,#Header_creative #Top_bar .top_bar_right .top-bar-right-button,#Header_creative #Top_bar .top_bar_right .top-bar-right-input{min-height:30px;margin:5px}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .contact_details{width:100%;text-align:center;margin-bottom:20px}#Header_creative #Action_bar .contact_details li{padding:0}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative #Action_bar .social-menu{float:none;text-align:center}#Header_creative #Action_bar .social-menu li{border-color:rgba(0,0,0,.1)}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}.header-open #Header_creative #Action_bar{display:block}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left;width:100%}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;z-index:203}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul.sub-menu li:not(:last-of-type) a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1240px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none;box-shadow:unset}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo,#Top_bar.is-sticky .custom-logo-link{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}.logo-sticky-width-auto #Top_bar.is-sticky #logo img.logo-sticky{width:auto}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky,.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li:not(.current-menu-item) > 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#Top_bar:not(.is-sticky){background-color:rgba(255,255,255,0.8)}}@media only screen and ( max-width:767px ){#Top_bar{background-color:#ffffff !important}#Action_bar{background-color:#007dc5 !important}#Action_bar .contact_details{color:#222222}#Action_bar .contact_details a{color:#0095eb}#Action_bar .contact_details a:hover{color:#007cc3}#Action_bar .social li a,#Action_bar .social-menu a{color:#bbbbbb!important}#Action_bar .social li a:hover,#Action_bar .social-menu a:hover{color:#777777!important}} form input.display-none{display:none!important} </style> <link rel='stylesheet' id='style-css' href='https://www.wyatt.com/wp-content/themes/betheme-child/style.css?ver=6.4.5' type='text/css' media='all' /> <style id='mfn-custom-inline-css' type='text/css'> @supports (-ms-ime-align: auto) { } span {font-family:"Open Sans", Arial, Tahoma, sans-serif !important;} @media only screen and (min-width: 1240px) { .header-classic #Header .top_bar_left {width:1306px;} #Top_bar .menu > li > a {padding:15px 5px;} #Top_bar.is-sticky .menu_wrapper .menu > li > a {padding:15px 5px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu{ padding-top:0px; padding-bottom:0px; width:92.5%!important; margin:0px 3%;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu .container {display:block;} /** Added 121024 **/ h1 { line-height: 44px !important; } .h2-underline {border-bottom: thick solid #ffb600; padding-bottom: 15px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu::before {content:' '; background:#fff; position:absolute; width:100vw; height:100%; margin-left: -50vw; left: 50%; } .mm-vertical #Top_bar .menu > li > .menu-overlap {z-index:1;background:rgba(0,0,0,0.4); position:absolute; width:100vw; height:100vh; margin-left: -50vw; left: 50%; cursor:hand; cursor:pointer;} .mm-vertical #Top_bar .menu > li.not-active a{color:#444444;} .mm-vertical #Top_bar .menu > li.not-active a::after {opacity:0;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title, .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title:hover {padding:5px 0px; background:transparent; color:#444444; text-transform:unset;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title:hover span {text-decoration:none ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu ul {background:transparent; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a {color:#007dc5; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a:hover {background:transparent;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a {padding:5px 0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a:hover {text-decoration:underline;} .mm-vertical #Top_bar .menu > li > ul {padding:20px 20px 20px 2px;} .mm-vertical #Top_bar .menu > li > ul a {padding:5px; border:0px; color:#007dc5;} .mm-vertical #Top_bar .menu > li > ul a:hover {background:transparent; } .mm-vertical #Top_bar .menu > li > ul a:hover span {text-decoration:underline ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li {padding-top:20px; padding-bottom:25px; border-right: 0px solid rgba(0, 0, 0, 0.05);} /*for parent title in sbmenu*/ .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 {padding-top:20px; line-height:30px; border-bottom:1px solid #ccc; font-weight:600;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 a{color:#444444;} /*for contact submenu*/ .mm-vertical #Top_bar .menu > li#menu-item-2437 li.mfn-megamenu-cols-2 li {} /*menu button css*/ .mm-vertical #Top_bar .menu > li#menu-item-5221 a span {background:#007dc5; color:#fff; border-radius:6px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-5221 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-5221 > a, #Top_bar.is-sticky .menu > li#menu-item-5221 > a span {line-height:30px; margin-top:0px;} .mm-vertical #Top_bar li#menu-item-6152 a {color:#000 !important; padding-top:0px !important; white-space:nowrap; color: #007dc5 !important; font-weight: 400;} /*set width of menu item*/ .mm-vertical #Top_bar li#menu-item-2341 {width:343px; padding-left: 80px;} .mm-vertical #Top_bar li#menu-item-2348 {width:278px;} .mm-vertical #Top_bar li#menu-item-2355 {width:288px;} .mm-vertical #Top_bar li#menu-item-2363 {width:240px;} .mm-vertical #Top_bar li#menu-item-2369 {width:541px; padding-left:70px;} .mm-vertical #Top_bar li#menu-item-2379 {width:340px;} .mm-vertical #Top_bar li#menu-item-2384 {width:280px;} .mm-vertical #Top_bar li#menu-item-2391 {width:350px; padding-left:80px;} .mm-vertical #Top_bar li#menu-item-2395 {width:310px;} .mm-vertical #Top_bar li#menu-item-2403 {width:310px;} .mm-vertical #Top_bar li#menu-item-2408 {width:210px;} .mm-vertical #Top_bar li#menu-item-5918 {width:487px; padding-left:115px;} .mm-vertical #Top_bar li#menu-item-5919 {width:387px;} .mm-vertical #Top_bar li#menu-item-5920 {width:280px;} .mm-vertical #Top_bar li#menu-item-2427 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-5235 {width:487px; padding-left:115px;} .mm-vertical #Top_bar li#menu-item-5765 {width:310px;} .mm-vertical #Top_bar li#menu-item-26131 {width:387px;} .mm-vertical #Top_bar li#menu-item-15082 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-15044 {width:397px;} } @media only screen and (max-width: 1239px) { #menu > ul > li > a, a.action_button, #overlay-menu ul li a { font-size: 15px; font-weight: 400; letter-spacing: 1px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar #menu ul li ul li a {font-size:16px;} .mm-vertical #Top_bar #menu ul li ul li ul li a {font-size:15px;} } /* Header */ .header-classic #Top_bar { border-bottom: 0px solid #e1e1e1;} .header-classic:not(.home) #Content { border-top: 0px solid #e1e1e1; } .header-classic.home .mfn-rev-slider { border-top: 1px solid #e1e1e1; } /*Action Bar*/ #Action_bar a {color:#fff;} /* Widget */ .widget-area:before { display: none; } .aside_left .widget-area { border: 0; } /* Icon box */ .icon_box.has_border { background: #fff; border: 1px solid #e1e1e1; padding: 40px 10%; } .icon_box.no_border {background: #fff; border:0px; padding: 40px 10%;} .icon_box.has_border:after {border:0px} /* Offer box */ .offer_box { background-color: #252525; padding: 20px 20px 5px; text-align: center; } .offer_box .offer_box_list { border-width: 1px 1px 0 1px; border-style: solid; border-color: #929292; font-weight: 700; font-size: 16px; letter-spacing: 2px; margin-bottom: 20px; } .offer_box .offer_box_list a { display: block; color: #fff; border-bottom: 1px solid #929292; padding: 18px 15px; word-wrap: break-word; } /* Footer */ /*#Footer { border-top: 1px solid #e1e1e1; }*/ #Footer .widget > h4 { font-size: 18px; font-weight: 300; margin-bottom: 20px; } #Footer .footer_copy { background: #465158; border-top: 0; } .before-footer {background: #f9f9f9;} #Footer .footer_copy .social { display: none; } /**/ .top-widgets-container {float:right} .top-widgets-container .widget {padding-bottom:0px; margin-bottom:0px; float:right;margin-top:0px;} .top-widgets-container .widget ul.menu{list-style:none; padding:0px;} .top-widgets-container .widget ul.menu li {float:left; padding:12px 0px;} .top-widgets-container .widget ul.menu li a{padding:0px 20px; border-right:1px solid #ccc; line-height:15px;} .top-widgets-container .widget ul.menu li:last-child a{border-right:0px;} .top-widgets-container .widget_search .icon-search-fine {font-size:24px; line-height:40px;} .top-widgets-container .widget_search input[type="text"] {width:82%; float:right; padding:5px 10px 5px 5px; border:0px; margin-top:7px; height:30px;min-height:30px;} #menu-top-navigation .cart a {padding:0px 20px; } #menu-top-navigation .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} .mobilecart {display:none; width:30px; float:right; margin-top:11px;} .mobilecart a { padding:0px; border:0px;} .mobilecart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; left:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { .top-widgets-container .widget {float:none;} .top-widgets-container .widget.widget_nav_menu {max-width:245px; margin:0px auto; clear:both;} .top-widgets-container .widget ul.menu li a {padding:0px 15px;} #menu-top-navigation .cart.menu-item-2449 {display:none;} #menu-top-navigation .cart a {padding:0px 10px;} .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .widget:after {display:none;} .top-widgets-container {float:none; margin:0px auto;} .top-widgets-container .widget_search input[type="text"] {width:80%;} .mobilecart {display:block;} .top-widgets-container .widget_search {width:254px; overflow:hidden;padding-bottom:20px; margin:0px auto;} .top-widgets-container .widget_search .searchform{width:210px; float:left;} } .icon-100 .image_wrapper img {width:150px; height:150px;} .down-heading, .heading {padding-left:17px;} @media only screen and (min-width:320px) and (max-width:767px) { .down-heading, .heading {padding:0px; text-align:center;} } .ui-tabs .ui-tabs-nav li {border-width:0px;} .page-id-2602 .ui-tabs .ui-tabs-nav li a {padding:14px 28px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li {border:0px; padding:5px 0px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {background-image:none; font-size:100%; color:#2991d6; padding:5px 10px 5px 20px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a:hover {text-decoration:underline;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a {color:#000; border-left:3px solid #2991d6; border-radius:0px !important; padding:5px 10px 5px 17px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:23%; border:0px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:76.7%; border:0px !important; padding-top:5px;} @media only screen and (min-width:768px) and (max-width:1239px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {font-size:100%;} } @media only screen and (min-width:320px) and (max-width:767px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:100%;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:100%; padding-top:15px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {-webkit-box-shadow:none;-moz-box-shadow:none;box-shadow:none;} } .ui-tabs .ui-tabs-nav li h3 {margin:0px; padding:0px;} .ui-tabs .ui-tabs-nav li h3 a { text-decoration: none; display: block; padding: 14px 30px !important; font-size: 100%; font-weight:initial; } .ui-tabs .ui-tabs-panel .icon_box {padding-bottom:2px;} .ui-tabs .ui-tabs-panel .column.one-fourth {width:25%;} .ui-tabs .ui-tabs-panel .column.one-second, .ui-tabs .ui-tabs-panel .column.one-fourth, .ui-tabs .ui-tabs-panel .column.one-third, .ui-tabs .ui-tabs-panel .column.two-third{margin-left:0px; margin-right:0px;} @media only screen and (min-width: 960px) and (max-width: 1239px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:10px 20px !important;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:5px 10px !important;} } /* Make Nav Subheadings unclickable */ .unclickable > a:hover { cursor: default;} .wp-caption-text { background: transparent; color: #075c97; font-size:90%;} .ls-wrapper h3 a {color: #075c97;} .column_sidebar_widget {float:none;} /*app notes*/ ul.clients.appnotes .client_wrapper {text-align:left; height:auto !important; line-height:auto !important; overflow:hidden;} ul.clients.appnotes li:nth-child(2n+1) {clear:both;} ul.clients.appnotes .client_wrapper img { position:relative; left:auto !important; top: auto !important; float:left; -webkit-transform: none!important; -moz-transform: none!important; transform: none!important; padding-right:20px; width:auto!important; } ul.clients.appnotes .client_wrapper h3 { line-height:20px; font-size:16px; letter-spacing:1px; padding-top:10px;} ul.clients.appnotes .client_wrapper a:hover {text-decoration:none;} @media only screen and (max-width: 640px) { ul.clients.appnotes li {width:100% !important; clear:both;} } .pager {padding-top:20px;} .pager .page-numbers {border:1px solid #ccc; padding:3px 5px;} .pager .prev.page-numbers, .pager .next.page-numbers {border:0px;} .alignleft {margin:3px 10px 10px 0px;} dl {clear:both;} .team.team_vertical img.scale-with-grid {width:100%;} .column_placeholder {margin-bottom:0px;} .icon-height img{max-height:50px;} .single .post-header {margin-left:0px; margin-right:0px;} .single .section-post-header .single-photo-wrapper.image {display:none;} .widget_mfn_menu ul li.menu-item-has-children > a::after {content: '\e868'; font-family: "mfn-icons"; right:2px; position:absolute; width:20px; height:15px; color:#ccc; } table th ul, table td ul {list-style-position:inside !important;} .sticky-white #Top_bar.is-sticky, .sticky-white #Top_bar.is-sticky #menu, #Top_bar.is-sticky {background:#fff; opacity:1;} /*Webinars latest posts*/ .parent-pageid-3663 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-3663 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-3663 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-3663 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .parent-pageid-3663 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-3663 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars*/ .page-id-2653 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-2653 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-2653 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-2653 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-2653 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .page-id-2653 .Latest_news ul li .desc_footer .date {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-2653 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-2653 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-2653 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Newsletter Archive*/ .page-id-3661 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-3661 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-3661 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-3661 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-3661 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li.category-newsletter-archive .desc h5 a{color:#007dc5;} .page-id-3661 .Latest_news ul li .desc_footer .date {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-3661 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-3661 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-3661 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Ask the Expert*/ .page-id-5348 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-5348 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-5348 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-5348 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc h5{margin-bottom:0px;} .page-id-5348 .Latest_news ul li.category-ask-the-expert .desc h5 a{color:#007dc5;} .page-id-5348 .Latest_news ul li .desc_footer {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .date {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-5348 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-5348 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-5348 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; font-weight:normal; line-height:20px;} /*accordion title as h3*/ .accordion .question .title h3 {margin-bottom:0px; } .accordion .question.active .title h3{color:#2991d6} /*column padding*/ .column, .columns {margin:0px 10px 40px;} .accordion .answer .one-second.column {width:47%;} .wrap .one-second.column {width:47%;} /*.column_column ul li {margin-bottom:2px;}*/ .single .post-header {margin-bottom:10px;} /* Appnote seach box*/ .widget_appnote_search_widget {clear:both; display:block; overflow:hidden;} .widget_appnote_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_appnote_search_widget .searchform {display:inline-block; float:left;} .widget_appnote_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_appnote_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_appnote_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_appnote_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*flat box*/ .flat_box .photo_wrapper .icon {display:none;} .flat_box .photo_wrapper img.photo {transform: translateX(0px)} .flat_box .desc_wrapper {margin-left:0px;} /*center images at user profile*/ .page-id-2504 .image_frame .image_wrapper {text-align:center} .page-id-2504.if-overlay .image_frame:hover .image_wrapper img, .page-id-2504.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .if-overlay .image_frame .image_wrapper .image_links {display:none;} /* Changes for ClickDimensions submit button */ a.button_theme, a.tp-button.button_theme, button, input[type="submit"], input[type="reset"], input[type="button"] { background-color: #0065a4 !important; color: #ffffff !important; font-family: inherit !important; /*padding:0px;*/ } /*isotope wrapper*/ .blog_wrapper.isotope_wrapper .post-item .post-footer {background:none;} .blog_wrapper.isotope_wrapper .post-item .post-footer .post-links{border:0px;} .blog_wrapper.isotope_wrapper .grid .post-photo-wrapper {border:0px;} /* product page*/ .page-id-2582 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-2582 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-2582 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .uk-margin-small-right {vertical-align:middle; margin-top:-2px;} .page-id-2647 .ui-tabs .ui-tabs-panel .column.one-second {margin-bottom:0px;} .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {border-color:transparent;} @media only screen and (min-width: 960px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {width:12%;} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:88%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper { width:25%} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:75%;} } @media only screen and (min-width: 768px) { .page-id-2640 .column.column_pricing_item {margin:0px 1% 40px;} } /* contact form 7 submit button*/ .wpcf7-form-control.wpcf7-submit {padding:10px 30px;} .wpcf7 .red {color:#990000;} .wpcf7 span.wpcf7-list-item {display:block; margin:0px;} .wpcf7 label {font-weight:400;} #lostusername {border:1px solid #ddd; padding:30px 20px 0px;} #lostusername .input {width:100%;} #lostusername .button {padding:10px 20px;} .ur-frontend-form button[type="submit"], .ur-frontend-form input[type="submit"] {float:none;} .page-id-2690.if-overlay .image_frame:hover .image_wrapper img, .page-id-2690.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-2690 .team .desc_wrapper hr {display:none;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-2690 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* webinars seach box*/ .widget_webinars_search_widget {clear:both; display:block; overflow:hidden;} .widget_webinars_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_webinars_search_widget .searchform {display:inline-block; float:left;} .widget_webinars_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_webinars_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_webinars_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_webinars_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*Recent Webinars nav issue fix*/ .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a:after {display:none;} /*login-in nav issue fix*/ .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* Contact Customer Support nav issue fix*/ .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* custom seach box*/ .search #Content .searchform {display:inline-block; float:left;} .search #Content .searchform h4{clear:both; padding:10px 0px; margin:0px;} .search #Content .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .search #Content .searchform select {-webkit-appearance:menulist; padding:7px 10px;} .search #Content .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .search #Content .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .search #Content .searchform input[type=submit] {margin:0px; margin-top:15px;} } .wpcf7-form .anr_captcha_field {position:absolute;} /*newsletter archive tables remove border*/ .post.category-newsletter-archive .post-wrapper-content table td {box-shadow:none; border:0px;} .be-ix-link-block {max-width:750px; text-align:center; margin:0px auto; padding-top:20px;} #Footer .widgets_wrapper .column {margin:0px 9px 0px;} @media only screen and (min-width: 768px) and (max-width: 959px) { #Footer .widgets_wrapper .column {margin:0px 5px 0px;} } /*Login page popup */ .page-id-5451.logged-in #pum-8728 {display:none !important;} #cookie-law-info-bar a {color:#fff; text-decoration:underline;} #cookie-law-info-bar a:hover {text-decoration:none;} #cookie-law-info-bar .cli-plugin-button {text-decoration:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { #cookie-law-info-again{display:none !important;} } /*After Multi Language SETUP*/ #Action_bar .column {overflow:visible;} .top-widgets-container .widget_search input[type="text"] {margin-top:5px;} .widget.widget_nav_menu::after, .widget.widget_icl_lang_sel_widget::after, .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .wpml-ls-legacy-dropdown-click {margin-top:6px; } .wpml-ls-legacy-dropdown-click a, .wpml-ls-legacy-dropdown-click a:hover, .wpml-ls-legacy-dropdown-click a:focus, .wpml-ls-legacy-dropdown-click .wpml-ls-current-language:hover>a {color:#626262 !important; border:0px;text-decoration:none;} .wpml-ls-legacy-dropdown-click .wpml-ls-sub-menu {z-index:999;} .top-widgets-container h2.title {display:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { .wpml-ls-legacy-dropdown-click {margin:6px auto 0px; } .top-widgets-container .widget_search {clear:both;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .wpml-ls-legacy-dropdown-click {width:auto;} } #menu-top-navigation-ch .cart a {padding:0px 20px; } #menu-top-navigation-ch .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ch .cart.menu-item-2449 {display:none;} #menu-top-navigation-ch .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-13087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-13087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-13087 > a, #Top_bar.is-sticky .menu > li#menu-item-13087 > a span {line-height:30px; margin-top:0px;} } .post-9911 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .post-9911 span.wpcf7-form-control-wrap{margin-bottom:20px;} .post-9911 .wpcf7-list-item-label{color:#000000;} .post-9911 .unique .one-third.column{width:30.333% !important;} .post-9911 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .post-9911 .wrap .one-second.column{width:32%;} .post-9911 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .post-9911 .wpcf7 span.wpcf7-list-item{width: 100%;} .post-9911 .unique .one-third.column{width:100% !important;} .post-9911 .wrap .one-second.column{width:100%;} .post-9911 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*remove meta from search-results*/ .search.search-results .post-item .post-meta {display:none;} .search.search-results .post-item .post-desc {padding-left:0px;} /*footer update*/ /*#Footer .widgets_wrapper {padding-bottom:10px;} #Footer .widgets_wrapper .column {margin-bottom:10px;} #Footer #custom_html-4 > h4 {text-align:center;} #Footer #custom_html-14 > h4 {text-align:center;} #Footer #custom_html-9 {max-width:160px; margin:0px auto; clear:both; padding-top:25px;} .footer-resources {max-width:80%; margin:0px auto; } .footer-resources li {float:left; line-height:28px;} .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:12%; clear:both;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:28%;} html[lang="zh-hans"] .footer-resources li:nth-child(1), html[lang="zh-hans"] .footer-resources li:nth-child(6){width:15%; clear:both;} html[lang="zh-hans"] .footer-resources li:nth-child(2), html[lang="zh-hans"] .footer-resources li:nth-child(7){width:25%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:17%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:18%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:25%;} @media only screen and (min-width:320px) and (max-width:1239px) { .footer-resources {max-width:60%; } .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:50%; clear:none;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:50%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:50%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:50%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:50%;} } @media only screen and (min-width:320px) and (max-width:767px) { .footer-resources {max-width:100%; } #Footer #custom_html-4 > h4 {text-align:left;} #Footer #custom_html-14 > h4 {text-align:left;} #Footer #custom_html-9 {max-width:100%;} } */ .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display:inline-block; text-decoration: none; padding: 0px 20px !important; border:0px; text-transform: uppercase; line-height:30px; font-size: 15px; font-weight: 400;letter-spacing: 1px;} @media only screen and (min-width:320px) and (max-width:767px) { .tabs_vertical.ui-tabs .ui-tabs-nav li h3, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active h3 {text-align:center;} .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover {margin:20px auto;} } /*Webinars latest posts zh-hans*/ .parent-pageid-11725 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-11725 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-11725 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-11725 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .parent-pageid-11725 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-11725 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars zh-hans*/ .page-id-11764 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-11764 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-11764 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-11764 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-11764 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .page-id-11764 .Latest_news ul li .desc_footer .date {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-11764 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-11764 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-11764 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Request Product Quote zh-hans*/ .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12227 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12227 .wpcf7-list-item-label{color:#000000;} .page-id-12227 .unique .one-third.column{width:29% !important;} .page-id-12227 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12227 .wrap .one-second.column{width:32%;} .page-id-12227 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12227 .unique .one-third.column{width:100% !important;} .page-id-12227 .wrap .one-second.column{width:100%;} .page-id-12227 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Request Product Info zh-hans*/ .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12612 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12612 .wpcf7-list-item-label{color:#000000;} .page-id-12612 .unique .one-third.column{width:29% !important;} .page-id-12612 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12612 .wrap .one-second.column{width:32%;} .page-id-12612 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12612 .unique .one-third.column{width:100% !important;} .page-id-12612 .wrap .one-second.column{width:100%;} .page-id-12612 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Team in zh-hans*/ .page-id-12002.if-overlay .image_frame:hover .image_wrapper img, .page-id-12002.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-12002 .team .desc_wrapper hr {display:none;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-12002 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* product page in zh-hans*/ .page-id-12156 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-12156 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-12156 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } /*Recent Webinars nav issue fix for zh-hans*/ .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a:after {display:none;} .large h3{font-size:22px; line-height:30px;} .small {margin-left:25px;} sup {display:inline-block; margin-top:-4px;} sub {display:inline-block; margin-bottom:-4px;} sup, sub {display:inline-table;} .ui-tabs-panel .one-second.column {width:50%;} .parent-pageid-2582 .ui-tabs-panel td {text-align:left !important;} .ls-borderlessdark3d .ls-shadow { display: none !important;} #menu-top-navigation-de .cart a {padding:0px 20px;} #menu-top-navigation-de .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-de .cart.menu-item-14543{display:none;} #menu-top-navigation-de .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-15087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-15087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-15087 > a, #Top_bar.is-sticky .menu > li#menu-item-15087 > a span {line-height:30px; margin-top:0px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar li#menu-item-12984 a {color:#000 !important; padding-top:20px !important;} .mm-vertical #Top_bar li#menu-item-14978 a {color:#000 !important; padding-top:20px !important;} } #Footer #custom_html-15 > h4 {text-align:center;} /* product page German */ .page-id-14845 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-14845 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-14845.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-14295.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-14845 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .parent-pageid-14845 .ui-tabs-panel td {text-align:left !important;} .button_align.align_left .button {text-align:left; padding:10px 0;} .button_align.align_left .as_tab {font-size: 18px; line-height: 20px; font-weight: 400; background:#fff; letter-spacing: 0px; color: #2991d6 !important; padding: 5px 10px 5px 20px;margin:0; } .button_align.align_left .button.as_tab:hover {text-decoration:underline !important; background-color:#fff!important;} .button_align.align_left .button.as_tab:after {display:none;} .button_align.align_left .button.as_tab.active {color: #000; border-left: 3px solid #2991d6; border-radius: 0px !important; padding: 5px 10px 5px 17px !important;} .button_align.align_left .button.as_tab .button_label {margin:0!important;} .button_align.align_left .button.as_tab_btn { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display: inline-block; text-decoration: none; padding: 0px 20px !important; border: 0px; text-transform: uppercase; line-height: 30px; font-size: 15px; font-weight: 400; letter-spacing: 1px; } /*Japan language*/ #Footer #custom_html-22 > h4 { text-align: center; } #menu-top-navigation-ja .cart a {padding:0px 20px;} #menu-top-navigation-ja .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ja .cart.menu-item-2449 {display:none;} #menu-top-navigation-ja .cart a {padding:0px 10px;} } .mm-vertical #Top_bar .menu > li#menu-item-18403 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-18403 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-18403 > a, #Top_bar.is-sticky .menu > li#menu-item-18403 > a span {line-height:30px; margin-top:0px;} @media only screen and (min-width: 1240px) { #Top_bar #menu-german-main-menu > li > a span:not(.description) { padding: 0 3px; font-size:14px; } } body, ul.timeline_items, .icon_box a .desc, .icon_box a:hover .desc, .feature_list ul li a, .list_item a, .list_item a:hover, .widget_recent_entries ul li a, .flat_box a, .flat_box a:hover, .story_box .desc, .content_slider.carousel ul li a .title, .content_slider.flat.description ul li .desc, .content_slider.flat.description ul li a .desc, .post-nav.minimal a i { color: #333333 !important; } .column_column ul, .column_column ol, .the_content_wrapper:not(.is-elementor) ul, .the_content_wrapper:not(.is-elementor) ol { color: #333333 !important; } .hero { margin-top: 20px; } .bg-hero { background: radial-gradient(circle at 100% 0,#ebf1f2 0% 30%,#0e81a7 100%); } .hero-full { min-height: 450px; background-color: rgba(0, 0, 0, 0.5); } .column, .columns { margin: 0px 8px 40px; } .image-container { position: relative; display: inline-block; /* oder 'block' je nach Layout */ } .ribbon { position: absolute; top: 10px; /* Abstand von oben */ left: -20px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #FF5733; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 5px 15px; /* Innenabstand */ transform: rotate(-0deg); /* Dreht das Ribbon */ transform-origin: 0 0; /* Setzt den Drehpunkt */ font-size: 14px; /* Schriftgröße */ box-shadow: 2px 2px 5px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .event-badge { position: relative; margin-right: 10px; border-radius: 6px; top: 0px; /* Abstand von oben */ left: 0px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #007dc5; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 2px 8px; /* Innenabstand */ font-size: 10px; /* Schriftgröße */ box-shadow: 1px 1px 2px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .btn-default { background: #007dc5; color: #fff; border-radius: 6px; line-height: 40px; margin-top: 8px; margin-left: 20px; padding: 0px 10px; } .wpcf7 form.sent .wpcf7-response-output { color: #ffffff; background-color: #46b450; } .jumpto { padding-top: 100px; /* Adjust this value to match your header's height */ margin-top: -100px; /* This should be the same value as padding-top but negative */ } .post-item { margin-bottom: 40px; position: relative; float: left; width: 100%; border: 1px solid #ccc; padding: 20px; background-color: #f8f8f8; } .post-links { background-color: #007dc5; border: 1px solid #ccc; padding: 5px 20px; } #field_1_18, #field_1_113, #field_1_20, #field_1_21, #field_1_22, #field_1_23, #field_1_24, #field_1_25, #field_1_26, #field_1_27, #field_1_49, #field_1_51, #field_1_52, #field_1_53, #field_1_54, #field_1_56, #field_1_57, #field_1_58, #field_1_59, #field_1_60, #field_1_62, #field_1_63, #field_1_64, #field_1_65, #field_1_67, #field_1_68, #field_1_70, #field_1_72, #field_1_98, #field_1_99, #field_1_100, #field_1_101, #field_1_102, #field_1_103, #field_1_104, #field_1_107, #field_1_108, #field_1_122, #field_1_123, #field_1_124, #field_1_125, #field_1_126, #field_1_127, #field_1_128, #field_1_136 { background-color: #E8E8E8; padding: 20px 30px; border: 1px solid #C4C4C4; border-bottom: 1px solid #C4C4C4; margin-top: -20px; } #field_1_73, 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font-size: 18px; font-weight: 600; margin-bottom: 20px; } /* new css */ .mcb-column-inner { margin: 0px; overflow:visible;} .one-sixth.column { width: 14.666%; } /* 1/6 */ .one-fifth.column { width: 18%; } /* 1/5 */ .one-fourth.column, .four.columns { width: 23%; } /* 1/4 */ .one-third.column { width: 31.333%; } /* 1/3 */ .two-fifth.column { width: 38%; } /* 2/5 */ .one-second.column { width: 48%; } /* 1/2 */ .three-fifth.column { width: 58%; } /* 3/5 */ .two-third.column { width: 64.666%; } /* 2/3 */ .three-fourth.column { width: 73%; } /* 3/4 */ .four-fifth.column { width: 78%; } /* 4/5 */ .five-sixth.column { width: 81.333%; } /* 5/6 */ .one.column { width: 98%; } /* 1/1 */ .wpcf7-form .column { margin-bottom: 10px;} .wpcf7-form .column { padding: 0px;} .wpcf7-form .column p{ margin: 0px;} .column-margin-0px.column { margin-bottom: 0 !important;} .column-margin-10px.column { margin-bottom: 0px !important;} .column-margin-20px.column { margin-bottom: 0px !important;} .page-pager, .post-pager { margin-bottom: 0!important;} .column.column_divider { margin-bottom: 0!important; height: auto!important;} #Header .container {padding-left:0; padding-right:0;} .accordion .question > .title > .acc-icon-plus, .accordion .question.active > .title > .acc-icon-minus { display: block;} .accordion .question > .title > .acc-icon-plus, .accordion .question .title > .acc-icon-minus {left:15px; top:16px;} .mcb-wrap-inner {display:block;} @media only screen and (max-width: 767px) { .section_wrapper .mcb-wrap, .section_wrapper .column, .container .column { width:100%!important; } } @media only screen and (min-width: 768px) { .be-ix-link-block .be-related-link-container .be-label {flex:0 0 17%;} .be-ix-link-block .be-related-link-container .be-list {flex:0 0 83%;} } ul.clients.appnotes .client_wrapper a, ul.clients.appnotes .client_wrapper .gs-wrapper { justify-content:left; } /*Top bar search*/ #Action_bar #global-searchfield-container .addsearch-searchfield input[type=search].icon {margin-bottom:0px; margin-top:4px; padding:5px 5px 5px 40px!important; height:30px;} #Action_bar #global-searchfield-container .addsearch-searchfield button {display:none;} #Action_bar .adds-components {padding:0px;} /*Top Shadow */ .header-classic #Top_bar { border-bottom: 0px solid #e1e1e1; box-shadow: rgba(0, 0, 0, 0.15) 0px 1.95px 2.6px; margin-bottom: 1px; } .sticky-submenu { position: -webkit-sticky; /* Für Safari */ position: sticky; top: 80px; z-index: 9999; /*background-color: #fff;*/ padding: 10px; /*border-bottom: 1px solid #ccc;*/ text-align: center; } .sticky-submenu ul { list-style-type: none; margin: 0; padding: 0; } .sticky-submenu ul li { display: inline; margin: 0 15px; } .sticky-submenu ul li a { text-decoration: none; /*color: #000;*/ color:#fff; padding: 5px; transition: border-bottom 0.3s; } .sticky-submenu ul li a.active, .sticky-submenu ul li a:hover { /*border-bottom: 2px solid #000;*/ border-bottom: 2px solid #fff; } .page-section { padding: 50px; margin-top: 50px; } .anchor-offset { display: block; position: relative; top: -60px; /* Höhe des sticky-submenu */ } .copyright a {padding:0px 7px;} .before-footer h4 {font-size:18px;} .before-footer .button {border-radius:4px;} .footer-button { padding: 10px 5px; border-radius: 5px; text-align: center; display: inline-block; width: 180px; border:1px solid #fff !important;} #Footer .widget_nav_menu ul { list-style-type: none; padding: 0px; background: none;} #Footer .widget_nav_menu ul a{padding:0px;} #Footer .widget_nav_menu {margin:20px 0px;} #Footer .one-fifth {width:16%;} #Footer .one-fifth:first-child {width:27%;} .social .icon_bar, .social .icon_bar span {width:auto !important; height:auto!important; line-height:0px!important; background-color:transparent; border:0px !important; color:#fff; font-size:24px; -webkit-box-shadow:none;box-shadow:none;} .social a:hover.icon_bar span { -webkit-transform: translateY(0%); -moz-transform: translateY(0%); transform: translateY(0%); } /*30aug2024*/ table tr:first-child td {box-shadow:none;} .btn_radius {border-radius: 4px;} #Top_bar .menu > li > a span:not(.description) {padding:0px 3px;} @media only screen and (min-width: 1300px) { #Top_bar .menu > li > a span:not(.description) {padding:0px 10px;} #Top_bar.is-sticky .menu > li > a span {padding:0px 15px;} } /** Added 11/14/24 **/ .scroll { text-decoration: none; transition: color 0.3s ease, font-weight 0.3s ease; } .scroll.active { font-weight: bold; } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-5221 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-15087 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-13087 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-18403 a span { background: #13b5ea !important; } } /** Added 11/18/24 **/ .header-section { border-bottom: 8px solid #fdbb30 !important; } .header-section .mcb-background-overlay{ } .solution-box { padding: 20px; border: 4px solid #EBEDEF; min-height: 150px; } .image_frame:hover .image_wrapper .mask { display:none; } .image_frame:hover .image_wrapper img, .image_frame:focus .image_wrapper img { top: 0 !important; -webkit-transform: scale(1) !important; -moz-transform: scale(1) !important; -o-transform: scale(1) !important; transform: scale(1) !important; -webkit-transition: none !important; transition: none !important; } .section-headline { border-bottom: thick solid #fdbb30; padding-bottom: 15px; font-size: 26px; } /** Page CTA Elements **/ .responsive-container { display: flex; flex-wrap: nowrap; border: 4px solid #EBEDEF; padding: 20px; align-items: flex-start; gap: 20px; width: 100%; } .responsive-container img { width: 100px; height: auto; flex-shrink: 0; } .responsive-container div { flex: 1; } /* 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class="searchform" action="https://www.wyatt.com/" > <div><input type="text" placeholder="Search..." value="" name="s" id="s" /> <a href="javascript:document.getElementById('searchform2').submit();" class="icon-search-fine"></a> </div> </form><div class="mobilecart"><a href="https://store.wyatt.com/"></a></div> </div> </div> </div> </div> </div> </header> </div>  <div id="Content" role="main"> <div class="content_wrapper clearfix"> <main class="sections_group"> <div class="entry-content" itemprop="mainContentOfPage"> <div class="mfn-builder-content mfn-default-content-buider"><section class="section mcb-section mfn-default-section mcb-section-bfceea66e header-section default-width" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-bfceea66e"><div class="wrap mcb-wrap mcb-wrap-25eb27eeb one tablet-one laptop-one mobile-one clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-25eb27eeb mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="wrap mcb-wrap mcb-wrap-ba05a9a06 one-second tablet-one-second laptop-one-second mobile-one mfn-nested-wrap clearfix" data-desktop-col="one-second" data-laptop-col="laptop-one-second" data-tablet-col="tablet-one-second" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-ba05a9a06 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-45970dff3 one laptop-one tablet-one mobile-one column_column" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-45970dff3 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h1 style="color: white;">Conformation</h1> <p style="font-size: 18px;">Light scattering determines branching in polymers and other measures of sub-micron shape or structure, entirely in solution.</p></div></div></div></div></div></div></div></div></section><section class="section mcb-section mfn-default-section mcb-section-wtezpv2rg default-width" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-wtezpv2rg"><div class="wrap mcb-wrap mcb-wrap-oij22iww one-fourth tablet-one-fourth laptop-one-fourth mobile-one sticky-desktop clearfix" data-desktop-col="one-fourth" data-laptop-col="laptop-one-fourth" data-tablet-col="tablet-one-fourth" data-mobile-col="mobile-one" style="--sticky-offset-desktop:110px" ><div class="mcb-wrap-inner mcb-wrap-inner-oij22iww mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="wrap mcb-wrap mcb-wrap-7rx60kstq one tablet-one laptop-one mobile-one mfn-item-custom-width mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-7rx60kstq mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-0czpty5ej one laptop-one tablet-one mobile-one column_table_of_contents" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-0czpty5ej mcb-item-table_of_contents-inner"><div class="table_of_content clearfix " data-tags="H2 H3"><div class="title"><h4 class="title-inner">Table of contents</h4></div><div class="table_of_content_wrapper"><ol class="mfn_toc_numbers"><li data-depth="1" ><i></i><a class="scroll" href="#introduction">Introduction</a></li><li><i></i><a class="scroll" href="#branching-ratio">Branching Ratio</a></li><li><i></i><a class="scroll" href="#shape-factor">Shape Factor</a></li><li><i></i><a class="scroll" href="#resources">Resources</a></li><li class="mfn_toc_nested" data-depth="2"><ol><li><i></i><a class="scroll" href="#application-notes">Application Notes</a></li><li><i></i><a class="scroll" href="#selected-references">Selected References</a></li></ol><li><i></i><a class="scroll" href="#instrumentation-for-conformation-analyses">Instrumentation for Conformation Analyses</a></li></ol></div></div> </div></div><div class="column mcb-column mcb-item-1enj3jrgw one laptop-one tablet-one mobile-one column_plain_text" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-1enj3jrgw mcb-item-plain_text-inner"><div class="desc"><a href="https://www.wyatt.com/products/request-product-info.html" class="sidebar-btn" target="_blank" rel="noopener noreferrer"> Request Product Information </a></div></div></div></div></div></div></div><div class="wrap mcb-wrap mcb-wrap-66aa78wgw three-fourth tablet-three-fourth laptop-three-fourth mobile-one clearfix" data-desktop-col="three-fourth" data-laptop-col="laptop-three-fourth" data-tablet-col="tablet-three-fourth" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-66aa78wgw mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div id="introduction" class="wrap mcb-wrap mcb-wrap-e5w1uye one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-e5w1uye mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-1fae3bbb8 one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-1fae3bbb8 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2 class="section-headline">Introduction</h2> <p>Smaller than the wavelength of visible light, it is notoriously difficult to determine the shape, structure or conformation of macromolecules and nanoparticles. While imaging techniques such as AFM and cryoTEM can in some cases provide detailed information, they require significant alterations to native solution conditions with deposition on a surface, and measure a very small, often unrepresentative ensemble. Light scattering offers non-invasive determination of conformation, in solution, over large and representative ensembles.</p> <p>The use of triple-detection GPC combining multi-angle static light scattering (MALS), differential viscometry (dVI) or dynamic light scattering (DLS), and differential refractometry (dRI), independently or in combination, provides quantitative analysis of:</p> <ul> <li>Branching ratio in polymers and dendrites</li> <li>The shape of protein aggregates or amyloid fibrils</li> <li>Empty or filled carrier nanoparticles</li> </ul></div></div></div></div></div><div id="branching-ratio" class="wrap mcb-wrap mcb-wrap-xpgkq9uht one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-xpgkq9uht mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-gkg5b6bjel one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-gkg5b6bjel mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2 class="section-headline">Branching Ratio</h2> <p><img style="margin: 10px; width: 400px; float: right;" title="Conformation" src="/wp-content/uploads/2019/05/conformation-graph.png" alt="Conformation" /></p> <p>Important properties of synthetic polymers such as stiffness, viscosity and solubility are governed by the branching ratio <i>g</i>. As outlined in the white paper <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-synth-polymers/branched-polymers-podzimek2013.pdf" target="_blank" rel="noopener">'Branching Revealed: Characterizing Molecular Structure in Synthetic and Natural Polymers by Multi-Angle Light Scattering' </a>by Dr. Stepan Podzimek, the branching ratio is most reliably determined from the slope <i>v</i> of the log-log plot of the rms radius <i>R</i><sub>g</sub> vs molar mass <i>M</i><sub>w</sub>. Since MALS determines both parameters - simultaneously and independently - downstream of a size-based separation technique such as SEC-MALS or FFF-MALS, it is the ideal technique for this purpose, as long as molecules are larger than about 10 nm in radius.</p> <p style="margin: 30px 20px 20px 0px; float: left;"><img title="Branched Polymer" src="/wp-content/uploads/2019/05/branched-polymer.jpg" alt="Branched Polymer" width="115px;" /></p> <p> </p> <p>For smaller molecules, the hydrodynamic radius <i>R</i><sub>h</sub> as determined by differential viscometry or online DLS may be substituted for <i>R</i><sub>g</sub>. The addition of a <a href="/products/instruments/viscostar-online-differential-viscometer.html">ViscoStar<sup>™</sup></a> differential viscometer to a MALS system introduces the parallel capability for dVI analysis to determine branching and the Mark-Houwink parameter.</p></div></div></div></div></div><div id="shape-factor" class="wrap mcb-wrap mcb-wrap-tw7dnrjl one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-tw7dnrjl mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-rf2ashakc one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-rf2ashakc mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2 class="section-headline">Shape Factor</h2> <p>For macromolecular assemblies besides branched polymers, the log-log plot of <i>R</i><sub>g</sub> vs. <i>M</i><sub>w</sub> is indicative of shape. Increasing values of the slope <i>v</i> progress from spherical, to random coil, to linear conformations of polymers and aggregates. For example, amorphous protein aggregates are distinguished from amyloid fibrils by the value of <i>v</i>, which is in the range of spherical/random coil for amorphous aggregates but much closer to the value expected for a linear arrangement in the case of amyloid fibrils.</p> <p>Similar information is obtained from the shape factor, <i>ρ</i> = <i>R</i><sub>g</sub>/<i>R</i><sub>h</sub>, for each macromolecular size fraction. The theoretical shape factor of ellipsoids, often taken to represent macromolecules and their assemblies or aggregates, is well known as a function of semi-axis ratio. Hence MALS with simultaneous, integrated DLS by means of the <a href="https://store.wyatt.com/shop/udawn/udawn3/wiq/">WyattQELS<sup>™</sup></a> module is an independent and orthogonal method to confirm the shape of an assembly or nanoparticle.</p> <p>When the analyte is known to have a particular shape, the shape factor provides structural information. For example, since liposomes are always spherical, the shape factor <i>ρ</i> clearly differentiates between empty liposomes and those filled with a substance such as a drug compound.</p> <p><img title="Conformation of Liposomes" src="/wp-content/uploads/2019/05/conformation-liposomes.png" alt="Conformation of Liposomes" width="400" /></p> <p><i>ρ</i> = ~1.00</p> <p><i>ρ</i> = ~0.77</p></div></div></div></div></div><div id="resources" class="wrap mcb-wrap mcb-wrap-avrfmk5k8 one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-avrfmk5k8 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-7z00g76ys one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-7z00g76ys mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2 class="section-headline">Resources</h2></div></div></div></div></div><div id="application-notes" class="wrap mcb-wrap mcb-wrap-txafemub5 one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-txafemub5 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-a547pus4c one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-a547pus4c mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>Application Notes</h3> <div style="display: flex; align-items: center; gap: 8px; margin-bottom: 10px;"><i class="fas fa-file-pdf" style="color: #8a8a8d; font-size: 22px;"></i> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-proteins/lens-conformation.pdf" target="_blank" rel="noopener">Conformational Variation of Eye Lens Protein</a></div> <div style="display: flex; align-items: center; gap: 8px; margin-bottom: 10px;"><i class="fas fa-file-pdf" style="color: #8a8a8d; font-size: 22px;"></i> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-synth-polymers/branched-polymers-podzimek2013.pdf" target="_blank" rel="noopener">Analysis of Polymer Branching</a></div> <div style="display: flex; align-items: center; gap: 8px; margin-bottom: 10px;"><i class="fas fa-file-pdf" style="color: #8a8a8d; font-size: 22px;"></i> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-biopolymers/hyperbranched-polyols-sec-mals-vi-poster.pdf" target="_blank" rel="noopener">Hyperbranched Polyols</a></div> <div style="display: flex; align-items: center; gap: 8px; margin-bottom: 10px;"><i class="fas fa-file-pdf" style="color: #8a8a8d; font-size: 22px;"></i> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-biopolymers/bottlebrush.pdf" target="_blank" rel="noopener">Bottle-Brush Polymers</a></div> <div style="display: flex; align-items: center; gap: 8px; margin-bottom: 10px;"><i class="fas fa-file-pdf" style="color: #8a8a8d; font-size: 22px;"></i> <a href="https://wyattfiles.s3-us-west-2.amazonaws.com/literature/app-notes/sec-mals-synth-polymers/branching-synthetic-rubbers.pdf" target="_blank" rel="noopener">Branching Analysis of Synthetic Rubbers: Polybutadiene and a Butadiene-Styrene Copolymer</a></div> <div style="display: flex; align-items: center; gap: 8px; margin-bottom: 10px;"><i class="fa-solid fa-arrow-up-right-from-square" style="color: #8a8a8d; font-size: 16px; opacity: 0.7;"></i> <a href="/library/application-notes/applications.html" target="_blank" rel="noopener">More Applications</a></div></div></div></div></div></div><div id="selected-references" class="wrap mcb-wrap mcb-wrap-ogjboirz one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-ogjboirz mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-qvdh6pkk one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-qvdh6pkk mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h3>Selected References</h3> <p>Ahmad, I. A. H.; Striegel, A. M. <a href="https://scholar.google.com/scholar?q=10.1007%2Fs00216-010-4118-5&btnG=&hl=en&as_sdt=0%2C5" target="_blank" rel="noopener">Influence of second virial coefficient and persistence length on dilute solution polymer conformation</a>. <i>Anal. Bioanal. Chem. </i> <b>2011,</b> <i>399</i>, 1515-1521.</p> <p>Peng, Y.; Zhang, L. <a href="https://scholar.google.com/scholar?q=10.1016%2FS0165-022X%2803%2900062-9&btnG=&hl=en&as_sdt=0%2C5" target="_blank" rel="noopener">Characterization of a polysaccharide-protein complex from <i>Ganoderma tsugae</i> mycelium by size-exclusion chromatography combined with laser light scattering</a>. <i>J. Biochem. Bioph. Meth. </i> <b>2003,</b> <i>56</i>, 243-252.</p> <p>Striegel, A. M. <a href="https://scholar.google.com/scholar?q=10.1016%2FS0165-022X%2803%2900054-X&btnG=&hl=en&as_sdt=0%2C5" target="_blank" rel="noopener">Architecture on the mechanochemical degradation of macromolecules</a>. <i>J. Biochem. Bioph. Meth. </i> <b>2003,</b> <i>56</i>, 117-139.</p> <p>Tarazona, M. P; Saiz, E. <a href="https://scholar.google.com/scholar?q=10.1016%2FS0165-022X%2803%2900075-7&btnG=&hl=en&as_sdt=0%2C5" target="_blank" rel="noopener">Combination of SEC/MALS experimental procedures and theoretical analysis for studying the solution properties of macromolecules</a>. <i>J. Biochem. Bioph. Meth. </i> <b>2003,</b> <i>56</i>, 95-116.</p> <p>Zhang, C.; Zhou, Y.; Liu, Q.; Li, S.; Perrier, S.; Zhao, Y. <a href="https://scholar.google.com/scholar?q=10.1021%2Fma1024736&btnG=&hl=en&as_sdt=0%2C5" target="_blank" rel="noopener">Facile synthesis of hyperbranched and star-shaped polymers by RAFT polymerization based on a polymerizable trithiocarbonate</a>. <i>Macromolecules </i> <b>2011,</b> <i>44</i>, 2034-2049.</p> <p><a href="/library/bibliography.html" target="_blank" rel="noopener">BIBLIOGRAPHIC SEARCH</a></p></div></div></div></div></div></div></div></div></section><section class="section mcb-section mfn-default-section mcb-section-7f591a0ce" id="instrumentation-for-conformation-analyses" style="padding-bottom:30px" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-7f591a0ce"><div class="wrap mcb-wrap mcb-wrap-266af37b4 one tablet-one laptop-one mobile-one valign-top clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-266af37b4 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-019a0be62 one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-019a0be62 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h2>Instrumentation for Conformation Analyses</h2> </div></div></div><div class="column mcb-column mcb-item-178483963 one laptop-one tablet-one mobile-one column_tabs" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-178483963 mcb-item-tabs-inner"><div class="jq-tabs tabs_wrapper tabs_vertical"><ul><li><h3><a href="#conformation-1">MALS</a></h3></li><li><h3><a href="#conformation-2">On-Line dVisc and dRI</a></h3></li><li><h3><a href="#conformation-3">Field-Flow Fractionation</a></h3></li><li><h3><a href="#conformation-4">DLS</a></h3></li><li><h3><a href="#conformation-5">Software</a></h3></li></ul><div id="conformation-1" ><p><b>MALS Detectors</b></p> <p><img alt=" dawn, molar mass determination, molar mass measurement, molar mass analysis, molecular weight measurement, molar mass characterization " height="" src="/wp-content/uploads/2019/05/DAWN-576x478-2.jpg" title="DAWN" width="300px" align="right"></p> <p><a href="/products/instruments/dawn-multi-angle-light-scattering-detector.html"><b>DAWN<sup>™</sup></b></a> - The most sensitive MALS detector available, anywhere. Incorporates detectors at 18 angles to determine molar masses from 200 Da to 1 GDa and radii from 10 – 500 nm.</p> <ul> <li>Standard option: ambient temperature</li> <li>Heated/cooled option: -15 °C to +150 °C</li> <li>High-temperature option: ambient to +210 °C</li> </ul> <p>The DAWN offers special options to handle fluorescent samples: fluorescence-blocking filters and an infrared, 785 nm laser.</p> <p><a href="/products/instruments/minidawn-multi-angle-light-scattering-detector-sec-mals.html"><br> <b>miniDAWN<sup>™</sup></b></a> - Second only to the DAWN in sensitivity. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 10 MDa and radii from 10 – 50 nm. Ambient only.</p> <p><a href="/products/instruments/microDawn-multi-angle-detector-uhplc-sec-mals.html"><b>microDAWN<sup>™</sup></b></a> - The first MALS detector for UHPLC, with interdetector dispersion as low as 1.5 µL. Incorporates detectors at 3 angles to determine molar masses from 200 Da to 20 MDa and radii from 10 – 50 nm. Ambient only.</p></div><div id="conformation-2" ><p><b>Viscometer</b></p> <p><img alt="ViscoStar" src="/wp-content/uploads/2019/05/ViscoStar-576x478.jpg" style="-webkit-margin-after: 1em;" title="ViscoStar" width="300px" align="right"></p> <p><a href="/products/instruments/viscostar-online-differential-viscometer.html"><b>ViscoStar<sup>™</sup></b></a> - A highly sensitive, on-line differential viscometer used in conjunction with SEC-MALS to determine the size and conformation of all types of biopolymers, synthetic polymers and even proteins and peptides.</p> <p>The ViscoStar incorporates multiple novel technologies to provide the highest sensitivity, stability and solvent compatibility of any available viscometer for GPC. Its ease-of-use and serviceability make it the perfect companion for Wyatt's DAWN<sup>™</sup> light scattering and Optilab<sup>™</sup> refractive index detectors. Temperature controlled from 4 °C to 70 °C. </p> <p><a href="/products/instruments/microviscostar.html"><b>microViscoStar<sup>™</sup></b></a> - Similar to the ViscoStar but designed specifically for use with UHPLC/APC.</p> <p><b>Refractive Index Detectors</b></p> <p><a href="/products/instruments/optilab-refractive-index-detector.html"><b>Optilab<sup>™</sup></b></a> - A unique on-line differential refractometer for measuring concentration of any macromolecule, regardless of chromophores. Temperature controlled from 4°C to 65°C. The high-concentration option accommodates protein concentration up to 180 mg/mL.</p> <p><a href="/products/instruments/microOptilab-uhplc-refractometer-sec-mals.html"><b>microOptilab™</b></a> - The first RI detector specifically designed for use with UHPLC systems. Compatible with all UHPLC systems.</p></div><div id="conformation-3" ><p><b>Field-Flow Fractionation Systems</b></p> <p><img alt="eclipse, molar mass determination, molar mass measurement, molar mass analysis, molecular weight measurement, molar mass characterization" height="" src="/wp-content/uploads/2020/10/Eclipse-Hero.jpg" title="Eclipse" width="300px" align="right"/></p> <p>Field-flow fractionation performs versatile separations of macromolecules and nanoparticles ranging from 1 to 1000 nm in diameter and beyond. FFF-MALS-DLS is the ideal means for obtaining distributions of molar mass and size, nanoparticle concentration and extended characterization of conformation and conjugate content.</p> <p><a href="/products/instruments/eclipse-field-flow-fractionation-system.html"><b>Eclipse™</b></a> - An FFF instrument offering superior performance, reliability and user experience. Supports the Dilution Control Module™ for enhanced sensitivity, and the SEC switching option for sharing the system between FFF and SEC separation modes.</p> <p><a href="https://jo.my/mobility-brochure" target="blank"><b>Mobility™ EAF4 system</b></a> - An add-on to an Eclipse which performs electrical/asymmetrical-flow FFF for determining charge and zeta potential distributions.</p> <p><a href="https://store.wyatt.com/?product_cat=eclipse&post_type=product&s=channel"><b>Eclipse Channels</b></a> - The Eclipse offers a wide selection of separation channels and membranes to accommodate a variety of analytical and even semi-preparative tasks.</p> </div><div id="conformation-4" ><p><b>Dynamic Light Scattering Detectors</b></p> <p><img alt="DynaPro NanoStar" src="/wp-content/uploads/2022/01/NanoStar_Hero_Display.jpg" title="DynaPro NanoStar" width="300px" align="right"></p> <p><a href="https://store.wyatt.com/shop/udawn/udawn3/wiq/"><b>WyattQELS™</b></a> - A dynamic light scattering (DLS) module which integrates into a DAWN, miniDAWN or microDAWN MALS detector to provide simultaneous DLS measurements in the same scattering volume.</p> <p><a href="/products/instruments/dynapro-nanostar-dynamic-light-scattering-detector.html"><b>DynaPro<sup>™</sup> NanoStar<sup>™</sup></b></a> - With sample volumes as small as 1.25 µL and temperature control spanning -15 °C to +150 °C, the NanoStar goes above and beyond traditional cuvette-based DLS instruments: it offers an optimized static light scattering detector in parallel to the DLS detection system which may be used for rapid assessment of conformation. The NanoStar does double duty as an online DLS detector by installing its optical collection fiber into a Wyatt MALS detector.</p></div><div id="conformation-5" ><p> <b>Software</b></p> <p><a href="/products/software/astra.html"><b>ASTRA<sup>™</sup></b></a> - Our comprehensive software solution for MALS analysis in chromatography, AF4 or batch mode. ASTRA is available in a 21 CFR Part 11 compliant version and offers additional options such as particle analysis and a research database. Provides multiple types of conformation analysis including conformation plots, Mark-Houwink-Sakurada parameters and shape factor.</p> <p><a href="/products/software/dynamics.html"><b><span itemprop="name">DYNAMICS<sup>™</sup></span></b></a> - Software for batch DLS measurements in the DynaPro instruments, as well as molar mass and <i>A</i><sub>2</sub> in the NanoStar and DynaPro Plate Reader. Calculates size and size distributions, and derives parameters such as the melting temperature <i>T</i><sub>m</sub>, the aggregation onset temperature <i>T</i><sub>agg</sub> and the diffusion interaction parameter <i>k</i><sub>D</sub>. Calculates axial ratio based on diffusion coefficient, specific volume and molar mass. DYNAMICS is available in standard and 21 CFR Part 11 compliant versions.</p></div></div></div></div></div></div></div></section></div><section class="section mcb-section the_content no_content"></section> <section class="section section-page-footer"> <div class="section_wrapper clearfix"> <div class="column one page-pager"> <div class="mcb-column-inner"> </div> </div> </div> </section> </div> </main> </div> </div>  <section class="section before-footer mcb-section mfn-default-section mcb-section-arexmxc3 default-width" style="padding-top:70px;"><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-arexmxc3"><div class="wrap mcb-wrap mcb-wrap-jana9ffxy container one-third tablet-one-third laptop-one-third mobile-one clearfix" data-desktop-col="one-third" data-laptop-col="laptop-one-third" data-tablet-col="tablet-one-third" data-mobile-col="mobile-one" style="padding-right:20px;"><div class="mcb-wrap-inner 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