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itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/73342921/A_validated_capillary_electrophoresis_method_to_check_for_batch_to_batch_consistency_during_recombinant_human_glycosylated_interleukin_7_production_campaigns">A validated capillary electrophoresis method to check for batch-to-batch consistency during recombinant human glycosylated interleukin-7 production campaigns</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This work reports the validation of a simple CZE method to be used in quality control of recombinant human glycosylated interleukin-7 (rhIL-7) batches produced in Chinese Hamster Ovary (CHO) cells. The separation buffer was a 25mM sodium... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_73342921" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This work reports the validation of a simple CZE method to be used in quality control of recombinant human glycosylated interleukin-7 (rhIL-7) batches produced in Chinese Hamster Ovary (CHO) cells. The separation buffer was a 25mM sodium borate at pH 10 containing 12mM diaminobutane (DAB) used as a dynamic coating agent of the capillary. This method allowed the separation of seven peaks ranging from low to high sialylated glycoforms. An extensive study on conditioning methods of the capillary has been conducted to yield repeatable results. Excellent RSD of EOF mobility (less than 0.6%) was obtained when conditioning included capillary equilibration under virtual analyses and storage in 0.1M NaOH overnight. Method specificity has been demonstrated to be able to discriminate different rhIL-7 glycoforms produced in CHO from formulation matrix. Linearity was demonstrated between 0.5 and 4mg/mL. LOQ was 0.5mg/mL. Repeatability (RSD&lt;1.4 and 3.3% for t(m) and A%, respectively), intermed...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/73342921" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="161612956" href="https://independent.academia.edu/AlahmadY">Yousef Alahmad</a><script data-card-contents-for-user="161612956" type="text/json">{"id":161612956,"first_name":"Yousef","last_name":"Alahmad","domain_name":"independent","page_name":"AlahmadY","display_name":"Yousef Alahmad","profile_url":"https://independent.academia.edu/AlahmadY?f_ri=15375","photo":"https://0.academia-photos.com/161612956/45334190/65999339/s65_yousef.alahmad.jpg"}</script></span></span></li><li class="js-paper-rank-work_73342921 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="73342921"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 73342921, container: ".js-paper-rank-work_73342921", }); 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href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="235383" href="https://www.academia.edu/Documents/in/Power_Supply">Power Supply</a>, <script data-card-contents-for-ri="235383" type="text/json">{"id":235383,"name":"Power Supply","url":"https://www.academia.edu/Documents/in/Power_Supply?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="239647" href="https://www.academia.edu/Documents/in/System_Development">System Development</a><script data-card-contents-for-ri="239647" type="text/json">{"id":239647,"name":"System 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Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=15375"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=15375"},{"id":1358142,"name":"Methyl Ester","url":"https://www.academia.edu/Documents/in/Methyl_Ester?f_ri=15375"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9880777" data-work_id="9880777" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" 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capillary electrophoresis using noncovalently coated capillaries","created_at":"2015-06-27T14:00:34.192-07:00","url":"https://www.academia.edu/13362065/Metabolic_analysis_of_body_fluids_by_capillary_electrophoresis_using_noncovalently_coated_capillaries?f_ri=15375","dom_id":"work_13362065","summary":null,"downloadable_attachments":[{"id":45431911,"asset_id":13362065,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32593393,"first_name":"Oleg","last_name":"Mayboroda","domain_name":"lumc","page_name":"OlegMayboroda","display_name":"Oleg Mayboroda","profile_url":"https://lumc.academia.edu/OlegMayboroda?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=15375","nofollow":false},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=15375","nofollow":false},{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":117555,"name":"Plasma","url":"https://www.academia.edu/Documents/in/Plasma?f_ri=15375","nofollow":false},{"id":549280,"name":"Reproducibility of Results","url":"https://www.academia.edu/Documents/in/Reproducibility_of_Results?f_ri=15375"},{"id":1146508,"name":"Body Fluids","url":"https://www.academia.edu/Documents/in/Body_Fluids?f_ri=15375"},{"id":1333997,"name":"Direct Injection","url":"https://www.academia.edu/Documents/in/Direct_Injection?f_ri=15375"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=15375"},{"id":1701407,"name":"Organic Acid","url":"https://www.academia.edu/Documents/in/Organic_Acid?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21559371 coauthored" data-work_id="21559371" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21559371/Pharmacokinetics_and_biodistribution_of_a_nucleotide_based_thrombin_inhibitor_in_rats">Pharmacokinetics and biodistribution of a nucleotide-based thrombin inhibitor in rats</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To characterize the pharmacokinetic and tissue distribution profiles of a nucleotide-based thrombin inhibitor (GS522, phosphodiester oligonucleotide, GGTTGGTGTGGTTGG) following intravenous administration to rats. Pharmacokinetic study: 10... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21559371" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To characterize the pharmacokinetic and tissue distribution profiles of a nucleotide-based thrombin inhibitor (GS522, phosphodiester oligonucleotide, GGTTGGTGTGGTTGG) following intravenous administration to rats. Pharmacokinetic study: 10 mg/kg, 20 mg/kg, 30 mg/kg (6 animals/dose) were administered to rats by rapid injection into the femoral vein. Blood samples were collected over a 45 minute period. Plasma concentrations of GS522 were determined using capillary gel electrophoresis with laser-induced fluorescence detection. Biodistribution Study: 10 mg/kg (400 microl, 31.46 microCi/ml) of 3H-GS522 was administered to rats by rapid injection into the femoral vein. The animals were sacrificed by decapitation at 1, 5, 10, 30, 60, 360 minutes post-dose (3 rats/point). Brain, blood, duodenum, eyes, heart, kidney, liver, lungs, muscle, pancreas, skin, spleen and vein samples were collected, processed and quantitated using liquid scintillation counting. The pharmacokinetic profile declines...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21559371" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="aa5a0b7805ba74b1f454eb8a53e69e82" rel="nofollow" data-download="{"attachment_id":42065793,"asset_id":21559371,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42065793/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42532264" href="https://independent.academia.edu/LReyderman">L. Reyderman</a><script data-card-contents-for-user="42532264" type="text/json">{"id":42532264,"first_name":"L.","last_name":"Reyderman","domain_name":"independent","page_name":"LReyderman","display_name":"L. Reyderman","profile_url":"https://independent.academia.edu/LReyderman?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-21559371">+1</span><div class="hidden js-additional-users-21559371"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/SStavchansky">S. Stavchansky</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-21559371'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-21559371').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_21559371 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21559371"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21559371, container: ".js-paper-rank-work_21559371", }); });</script></li><li class="js-percentile-work_21559371 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21559371; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_21559371"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_21559371 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="21559371"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21559371; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21559371]").text(description); $(".js-view-count-work_21559371").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21559371").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="21559371"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="95655" href="https://www.academia.edu/Documents/in/Pharmaceutical">Pharmaceutical</a>, <script data-card-contents-for-ri="95655" type="text/json">{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="375054" href="https://www.academia.edu/Documents/in/Rats">Rats</a>, <script data-card-contents-for-ri="375054" type="text/json">{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="809882" href="https://www.academia.edu/Documents/in/Base_Sequence">Base Sequence</a><script data-card-contents-for-ri="809882" type="text/json">{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21559371]'), work: {"id":21559371,"title":"Pharmacokinetics and biodistribution of a nucleotide-based thrombin inhibitor in rats","created_at":"2016-02-04T10:07:00.502-08:00","url":"https://www.academia.edu/21559371/Pharmacokinetics_and_biodistribution_of_a_nucleotide_based_thrombin_inhibitor_in_rats?f_ri=15375","dom_id":"work_21559371","summary":"To characterize the pharmacokinetic and tissue distribution profiles of a nucleotide-based thrombin inhibitor (GS522, phosphodiester oligonucleotide, GGTTGGTGTGGTTGG) following intravenous administration to rats. Pharmacokinetic study: 10 mg/kg, 20 mg/kg, 30 mg/kg (6 animals/dose) were administered to rats by rapid injection into the femoral vein. Blood samples were collected over a 45 minute period. Plasma concentrations of GS522 were determined using capillary gel electrophoresis with laser-induced fluorescence detection. Biodistribution Study: 10 mg/kg (400 microl, 31.46 microCi/ml) of 3H-GS522 was administered to rats by rapid injection into the femoral vein. The animals were sacrificed by decapitation at 1, 5, 10, 30, 60, 360 minutes post-dose (3 rats/point). Brain, blood, duodenum, eyes, heart, kidney, liver, lungs, muscle, pancreas, skin, spleen and vein samples were collected, processed and quantitated using liquid scintillation counting. The pharmacokinetic profile declines...","downloadable_attachments":[{"id":42065793,"asset_id":21559371,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42532264,"first_name":"L.","last_name":"Reyderman","domain_name":"independent","page_name":"LReyderman","display_name":"L. Reyderman","profile_url":"https://independent.academia.edu/LReyderman?f_ri=15375","photo":"/images/s65_no_pic.png"},{"id":42738918,"first_name":"S.","last_name":"Stavchansky","domain_name":"independent","page_name":"SStavchansky","display_name":"S. Stavchansky","profile_url":"https://independent.academia.edu/SStavchansky?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical?f_ri=15375","nofollow":false},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=15375","nofollow":false},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=15375","nofollow":false},{"id":1597877,"name":"Nucleotides","url":"https://www.academia.edu/Documents/in/Nucleotides?f_ri=15375"},{"id":1649745,"name":"Oligonucleotides","url":"https://www.academia.edu/Documents/in/Oligonucleotides?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13480877 coauthored" data-work_id="13480877" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13480877/Allele_frequencies_of_the_new_European_Standard_Set_ESS_loci_in_the_Italian_population">Allele frequencies of the new European Standard Set (ESS) loci in the Italian population</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13480877" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ad13eb0337fb22702223403cf79212da" rel="nofollow" data-download="{"attachment_id":45290970,"asset_id":13480877,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45290970/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32695987" href="https://independent.academia.edu/ElenaPilli">Elena Pilli</a><script data-card-contents-for-user="32695987" type="text/json">{"id":32695987,"first_name":"Elena","last_name":"Pilli","domain_name":"independent","page_name":"ElenaPilli","display_name":"Elena Pilli","profile_url":"https://independent.academia.edu/ElenaPilli?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13480877">+1</span><div class="hidden js-additional-users-13480877"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/AlessandroBosetti">Alessandro Bosetti</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13480877'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13480877').html(); } } new 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container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_13480877 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="13480877"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13480877; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13480877]").text(description); $(".js-view-count-work_13480877").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_13480877").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="13480877"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="4480" href="https://www.academia.edu/Documents/in/Population_Genetics">Population Genetics</a>, <script data-card-contents-for-ri="4480" type="text/json">{"id":4480,"name":"Population Genetics","url":"https://www.academia.edu/Documents/in/Population_Genetics?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="45213" href="https://www.academia.edu/Documents/in/Italy">Italy</a>, <script data-card-contents-for-ri="45213" type="text/json">{"id":45213,"name":"Italy","url":"https://www.academia.edu/Documents/in/Italy?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13480877]'), work: {"id":13480877,"title":"Allele frequencies of the new European Standard Set (ESS) loci in the Italian population","created_at":"2015-07-01T03:51:43.668-07:00","url":"https://www.academia.edu/13480877/Allele_frequencies_of_the_new_European_Standard_Set_ESS_loci_in_the_Italian_population?f_ri=15375","dom_id":"work_13480877","summary":null,"downloadable_attachments":[{"id":45290970,"asset_id":13480877,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32695987,"first_name":"Elena","last_name":"Pilli","domain_name":"independent","page_name":"ElenaPilli","display_name":"Elena Pilli","profile_url":"https://independent.academia.edu/ElenaPilli?f_ri=15375","photo":"/images/s65_no_pic.png"},{"id":32761368,"first_name":"Alessandro","last_name":"Bosetti","domain_name":"independent","page_name":"AlessandroBosetti","display_name":"Alessandro Bosetti","profile_url":"https://independent.academia.edu/AlessandroBosetti?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4480,"name":"Population Genetics","url":"https://www.academia.edu/Documents/in/Population_Genetics?f_ri=15375","nofollow":false},{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":45213,"name":"Italy","url":"https://www.academia.edu/Documents/in/Italy?f_ri=15375","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=15375","nofollow":false},{"id":75826,"name":"Europe","url":"https://www.academia.edu/Documents/in/Europe?f_ri=15375"},{"id":80414,"name":"Mathematical Sciences","url":"https://www.academia.edu/Documents/in/Mathematical_Sciences?f_ri=15375"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=15375"},{"id":134041,"name":"DNA fingerprinting","url":"https://www.academia.edu/Documents/in/DNA_fingerprinting?f_ri=15375"},{"id":371425,"name":"Electrophoresis","url":"https://www.academia.edu/Documents/in/Electrophoresis?f_ri=15375"},{"id":956761,"name":"Republic of Macedonia","url":"https://www.academia.edu/Documents/in/Republic_of_Macedonia?f_ri=15375"},{"id":1300632,"name":"ESS","url":"https://www.academia.edu/Documents/in/ESS?f_ri=15375"},{"id":1487366,"name":"Allele Frequency","url":"https://www.academia.edu/Documents/in/Allele_Frequency?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_58117560" data-work_id="58117560" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/58117560/Molecular_interactions_between_warfarin_and_human_HSA_or_bovine_BSA_serum_albumin_evaluated_by_isothermal_titration_calorimetry_ITC_fluorescence_spectrometry_FS_and_frontal_analysis_capillary_electrophoresis_FA_CE_">Molecular interactions between warfarin and human (HSA) or bovine (BSA) serum albumin evaluated by isothermal titration calorimetry (ITC), fluorescence spectrometry (FS) and frontal analysis capillary electrophoresis (FA/CE)</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Interaction thermodynamics between warfarin, a very popular anticoagulant, and Sudlow I binding site of human (HSA) or bovine (BSA) serum albumin have been examined in strictly controlled experimental conditions (HEPES buffer 50 mM, pH... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_58117560" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Interaction thermodynamics between warfarin, a very popular anticoagulant, and Sudlow I binding site of human (HSA) or bovine (BSA) serum albumin have been examined in strictly controlled experimental conditions (HEPES buffer 50 mM, pH 7.4 and 25 °C) by means of isothermal titration calorimetry (ITC), fluorescence spectrometry (FS) and frontal analysis capillary electrophoresis (FA/CE). Each technique is based on measurements of a different property of the biochemical system, and then the results allow a critical discussion about the suitability of each approach to estimate the drug-protein binding parameters. The strongest interaction step is properly evaluated by the three assayed approaches being the derived binding constants strongly consistent: from 4 × 104 to 7 × 104 for HSA and from 0.8 × 105 to 1.2 × 105 for BSA. Binding enthalpy variations also show consistent results: -5.4 and -5.6 Kcal/mol for HSA and -4.3 and -3.7 Kcal/mol for BSA, as measured by ITC and FS, respectively...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/58117560" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="212c6ff81363872f276e4df9cbac947f" rel="nofollow" data-download="{"attachment_id":72685204,"asset_id":58117560,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72685204/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38268147" href="https://barcelona.academia.edu/ElisabethBosch">Elisabeth Bosch</a><script data-card-contents-for-user="38268147" type="text/json">{"id":38268147,"first_name":"Elisabeth","last_name":"Bosch","domain_name":"barcelona","page_name":"ElisabethBosch","display_name":"Elisabeth Bosch","profile_url":"https://barcelona.academia.edu/ElisabethBosch?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_58117560 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="58117560"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 58117560, container: ".js-paper-rank-work_58117560", }); 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$(".js-view-count[data-work-id=58117560]").text(description); $(".js-view-count-work_58117560").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_58117560").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="58117560"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="522" href="https://www.academia.edu/Documents/in/Thermodynamics">Thermodynamics</a>, <script data-card-contents-for-ri="522" type="text/json">{"id":522,"name":"Thermodynamics","url":"https://www.academia.edu/Documents/in/Thermodynamics?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="524" href="https://www.academia.edu/Documents/in/Analytical_Chemistry">Analytical Chemistry</a>, <script data-card-contents-for-ri="524" type="text/json">{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="96893" href="https://www.academia.edu/Documents/in/Calibration">Calibration</a><script data-card-contents-for-ri="96893" type="text/json">{"id":96893,"name":"Calibration","url":"https://www.academia.edu/Documents/in/Calibration?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=58117560]'), work: {"id":58117560,"title":"Molecular interactions between warfarin and human (HSA) or bovine (BSA) serum albumin evaluated by isothermal titration calorimetry (ITC), fluorescence spectrometry (FS) and frontal analysis capillary electrophoresis (FA/CE)","created_at":"2021-10-15T06:31:29.102-07:00","url":"https://www.academia.edu/58117560/Molecular_interactions_between_warfarin_and_human_HSA_or_bovine_BSA_serum_albumin_evaluated_by_isothermal_titration_calorimetry_ITC_fluorescence_spectrometry_FS_and_frontal_analysis_capillary_electrophoresis_FA_CE_?f_ri=15375","dom_id":"work_58117560","summary":"Interaction thermodynamics between warfarin, a very popular anticoagulant, and Sudlow I binding site of human (HSA) or bovine (BSA) serum albumin have been examined in strictly controlled experimental conditions (HEPES buffer 50 mM, pH 7.4 and 25 °C) by means of isothermal titration calorimetry (ITC), fluorescence spectrometry (FS) and frontal analysis capillary electrophoresis (FA/CE). Each technique is based on measurements of a different property of the biochemical system, and then the results allow a critical discussion about the suitability of each approach to estimate the drug-protein binding parameters. The strongest interaction step is properly evaluated by the three assayed approaches being the derived binding constants strongly consistent: from 4 × 104 to 7 × 104 for HSA and from 0.8 × 105 to 1.2 × 105 for BSA. Binding enthalpy variations also show consistent results: -5.4 and -5.6 Kcal/mol for HSA and -4.3 and -3.7 Kcal/mol for BSA, as measured by ITC and FS, respectively...","downloadable_attachments":[{"id":72685204,"asset_id":58117560,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38268147,"first_name":"Elisabeth","last_name":"Bosch","domain_name":"barcelona","page_name":"ElisabethBosch","display_name":"Elisabeth Bosch","profile_url":"https://barcelona.academia.edu/ElisabethBosch?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":522,"name":"Thermodynamics","url":"https://www.academia.edu/Documents/in/Thermodynamics?f_ri=15375","nofollow":false},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=15375","nofollow":false},{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":96893,"name":"Calibration","url":"https://www.academia.edu/Documents/in/Calibration?f_ri=15375","nofollow":false},{"id":189949,"name":"Anticoagulants","url":"https://www.academia.edu/Documents/in/Anticoagulants?f_ri=15375"},{"id":400209,"name":"Warfarin","url":"https://www.academia.edu/Documents/in/Warfarin?f_ri=15375"},{"id":549280,"name":"Reproducibility of Results","url":"https://www.academia.edu/Documents/in/Reproducibility_of_Results?f_ri=15375"},{"id":653665,"name":"Protein Conformation","url":"https://www.academia.edu/Documents/in/Protein_Conformation?f_ri=15375"},{"id":677739,"name":"Calorimetry","url":"https://www.academia.edu/Documents/in/Calorimetry?f_ri=15375"},{"id":881608,"name":"Bovine Serum Albumin","url":"https://www.academia.edu/Documents/in/Bovine_Serum_Albumin?f_ri=15375"},{"id":967839,"name":"Structure activity Relationship","url":"https://www.academia.edu/Documents/in/Structure_activity_Relationship?f_ri=15375"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding?f_ri=15375"},{"id":2637271,"name":"Reference standards","url":"https://www.academia.edu/Documents/in/Reference_standards?f_ri=15375"},{"id":2898895,"name":"binding sites","url":"https://www.academia.edu/Documents/in/binding_sites?f_ri=15375"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_2081812" data-work_id="2081812" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/2081812/A_method_to_size_DNA_fragments_from_50_to_800_bp_on_a_DNA_analyser">A method to size DNA fragments from 50 to 800 bp on a DNA analyser</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/2081812" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7956ee6bda7bc3eac213d3a1803bc474" rel="nofollow" data-download="{"attachment_id":50769959,"asset_id":2081812,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen 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Feldheim","profile_url":"https://fieldmuseum.academia.edu/KevinFeldheim?f_ri=15375","photo":"https://0.academia-photos.com/2717901/875067/1092331/s65_kevin.feldheim.jpg"}</script></span></span></li><li class="js-paper-rank-work_2081812 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="2081812"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 2081812, container: ".js-paper-rank-work_2081812", }); });</script></li><li class="js-percentile-work_2081812 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 2081812; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_2081812"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_2081812 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="2081812"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 2081812; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=2081812]").text(description); $(".js-view-count-work_2081812").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_2081812").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="2081812"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">3</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2256666" href="https://www.academia.edu/Documents/in/DNA_fragmentation">DNA fragmentation</a><script data-card-contents-for-ri="2256666" type="text/json">{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=2081812]'), work: {"id":2081812,"title":"A method to size DNA fragments from 50 to 800 bp on a DNA analyser","created_at":"2012-11-02T14:34:54.388-07:00","url":"https://www.academia.edu/2081812/A_method_to_size_DNA_fragments_from_50_to_800_bp_on_a_DNA_analyser?f_ri=15375","dom_id":"work_2081812","summary":null,"downloadable_attachments":[{"id":50769959,"asset_id":2081812,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":2717901,"first_name":"Kevin","last_name":"Feldheim","domain_name":"fieldmuseum","page_name":"KevinFeldheim","display_name":"Kevin Feldheim","profile_url":"https://fieldmuseum.academia.edu/KevinFeldheim?f_ri=15375","photo":"https://0.academia-photos.com/2717901/875067/1092331/s65_kevin.feldheim.jpg"}],"research_interests":[{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=15375","nofollow":false},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=15375","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_12170390" data-work_id="12170390" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/12170390/Study_of_molar_response_of_dextrans_in_electrochemical_detection">Study of molar response of dextrans in electrochemical detection</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">In this work, a methodological approach is reported, aimed at assessing the electrochemical response of some model gluco-oligosaccharides (dextrans). Such strategy is based on the complementary use of both anion-exchange chromatography... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_12170390" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In this work, a methodological approach is reported, aimed at assessing the electrochemical response of some model gluco-oligosaccharides (dextrans). Such strategy is based on the complementary use of both anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD) and capillary zone electrophoresis coupled with UV detection (CZE–UV). Unlike HPAEC–PAD, CZE–UV required derivatization with a chromophoric dye (i.e., 8-aminonaphtalene-1,3,6-trisulphonic acid, ANTS) to enhance UV response and separation selectivity. From the comparison between chromophore response and PAD signal, the reliability of HPAEC–PAD for quantitative evaluation of dextran mixtures containing mainly oligomers with polymerization degree (DP) up to 18 could be proved, due to the fairly constant molar response. For higher DPs (up to 41), a maximum in the trend of the molar responses was observed followed by a steep decrease for DPs higher than about 30–35; indeed, an underestimation of weight-average...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/12170390" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a4824a190e6031fc179f18c776d037ca" rel="nofollow" data-download="{"attachment_id":46323488,"asset_id":12170390,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46323488/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="30578857" href="https://independent.academia.edu/SPaoletti">Sergio Paoletti</a><script data-card-contents-for-user="30578857" type="text/json">{"id":30578857,"first_name":"Sergio","last_name":"Paoletti","domain_name":"independent","page_name":"SPaoletti","display_name":"Sergio Paoletti","profile_url":"https://independent.academia.edu/SPaoletti?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_12170390 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="12170390"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 12170390, container: ".js-paper-rank-work_12170390", }); });</script></li><li class="js-percentile-work_12170390 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 12170390; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_12170390"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_12170390 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="12170390"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 12170390; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=12170390]").text(description); $(".js-view-count-work_12170390").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_12170390").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="12170390"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="923" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4748" href="https://www.academia.edu/Documents/in/Electrochemistry">Electrochemistry</a>, <script data-card-contents-for-ri="4748" type="text/json">{"id":4748,"name":"Electrochemistry","url":"https://www.academia.edu/Documents/in/Electrochemistry?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a><script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=12170390]'), work: {"id":12170390,"title":"Study of molar response of dextrans in electrochemical detection","created_at":"2015-04-30T15:14:47.462-07:00","url":"https://www.academia.edu/12170390/Study_of_molar_response_of_dextrans_in_electrochemical_detection?f_ri=15375","dom_id":"work_12170390","summary":"In this work, a methodological approach is reported, aimed at assessing the electrochemical response of some model gluco-oligosaccharides (dextrans). Such strategy is based on the complementary use of both anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD) and capillary zone electrophoresis coupled with UV detection (CZE–UV). Unlike HPAEC–PAD, CZE–UV required derivatization with a chromophoric dye (i.e., 8-aminonaphtalene-1,3,6-trisulphonic acid, ANTS) to enhance UV response and separation selectivity. From the comparison between chromophore response and PAD signal, the reliability of HPAEC–PAD for quantitative evaluation of dextran mixtures containing mainly oligomers with polymerization degree (DP) up to 18 could be proved, due to the fairly constant molar response. For higher DPs (up to 41), a maximum in the trend of the molar responses was observed followed by a steep decrease for DPs higher than about 30–35; indeed, an underestimation of weight-average...","downloadable_attachments":[{"id":46323488,"asset_id":12170390,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":30578857,"first_name":"Sergio","last_name":"Paoletti","domain_name":"independent","page_name":"SPaoletti","display_name":"Sergio Paoletti","profile_url":"https://independent.academia.edu/SPaoletti?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=15375","nofollow":false},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=15375","nofollow":false},{"id":4748,"name":"Electrochemistry","url":"https://www.academia.edu/Documents/in/Electrochemistry?f_ri=15375","nofollow":false},{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":203965,"name":"Ion Exchange Chromatography","url":"https://www.academia.edu/Documents/in/Ion_Exchange_Chromatography?f_ri=15375"},{"id":246560,"name":"High Pressure Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Pressure_Liquid_Chromatography?f_ri=15375"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=15375"},{"id":326380,"name":"Liquid Chromatography / Electrospray Ionization Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Liquid_Chromatography_Electrospray_Ionization_Mass_Spectrometry?f_ri=15375"},{"id":695018,"name":"Molecular weight","url":"https://www.academia.edu/Documents/in/Molecular_weight?f_ri=15375"},{"id":1222271,"name":"Quantitative Evaluation","url":"https://www.academia.edu/Documents/in/Quantitative_Evaluation?f_ri=15375"},{"id":1431843,"name":"Molecular Weight Distribution","url":"https://www.academia.edu/Documents/in/Molecular_Weight_Distribution?f_ri=15375"},{"id":1603130,"name":"Amperometry","url":"https://www.academia.edu/Documents/in/Amperometry?f_ri=15375"},{"id":2015155,"name":"Weighted Averaging","url":"https://www.academia.edu/Documents/in/Weighted_Averaging?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_17950764" data-work_id="17950764" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/17950764/A_new_approach_for_screening_for_Y_microdeletions_capillary_electrophoresis_combined_with_fluorescent_multiplex_PCR">A new approach for screening for Y microdeletions: capillary electrophoresis combined with fluorescent multiplex PCR</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To apply capillary electrophoresis for rapid screening for Y microdeletions A set of 25 specific sequence tagged sites that cover the azoospermia factor a, b, and c regions of the Y chromosome was amplified in 5 fluorescent multiplex sets... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_17950764" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To apply capillary electrophoresis for rapid screening for Y microdeletions A set of 25 specific sequence tagged sites that cover the azoospermia factor a, b, and c regions of the Y chromosome was amplified in 5 fluorescent multiplex sets each including 5 primer pairs. One of the primers of each pair was labeled with a fluorescent tag attached to the 5&#39;-end. After PCR amplification, analysis of the obtained PCR products was performed using capillary electrophoresis (ABI Prism 3100 Genetic Analyzer). The method was employed to determine Y microdeletions in azoospermic (n = 49) and severe oligozoospermic (n = 149) men. The number of PCR cycle (from 45 to 30) and the amount of DNA template (20-fold) used in fluorescent multiplex PCR were reduced because of the high sensitivity of capillary electrophoresis. Approximately 1000 multiplex PCR sets from 198 patients were analyzed simultaneously within 50 h. Y microdeletions were found in 3 out of the 198 azoospermic or severe oligozoosp...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17950764" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f5ff24860709bded5f3fad533cdbe4ec" rel="nofollow" data-download="{"attachment_id":39795447,"asset_id":17950764,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/39795447/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37868634" href="https://independent.academia.edu/PinarBor">Pinar Bor</a><script data-card-contents-for-user="37868634" type="text/json">{"id":37868634,"first_name":"Pinar","last_name":"Bor","domain_name":"independent","page_name":"PinarBor","display_name":"Pinar Bor","profile_url":"https://independent.academia.edu/PinarBor?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_17950764 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="17950764"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 17950764, container: ".js-paper-rank-work_17950764", }); 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$(".js-view-count[data-work-id=17950764]").text(description); $(".js-view-count-work_17950764").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_17950764").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="17950764"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="156" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="118339" href="https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction">Polymerase Chain Reaction</a>, <script data-card-contents-for-ri="118339" type="text/json">{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="178928" href="https://www.academia.edu/Documents/in/Male_Infertility">Male Infertility</a><script data-card-contents-for-ri="178928" type="text/json">{"id":178928,"name":"Male Infertility","url":"https://www.academia.edu/Documents/in/Male_Infertility?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=17950764]'), work: {"id":17950764,"title":"A new approach for screening for Y microdeletions: capillary electrophoresis combined with fluorescent multiplex PCR","created_at":"2015-11-08T04:21:57.210-08:00","url":"https://www.academia.edu/17950764/A_new_approach_for_screening_for_Y_microdeletions_capillary_electrophoresis_combined_with_fluorescent_multiplex_PCR?f_ri=15375","dom_id":"work_17950764","summary":"To apply capillary electrophoresis for rapid screening for Y microdeletions A set of 25 specific sequence tagged sites that cover the azoospermia factor a, b, and c regions of the Y chromosome was amplified in 5 fluorescent multiplex sets each including 5 primer pairs. One of the primers of each pair was labeled with a fluorescent tag attached to the 5\u0026#39;-end. After PCR amplification, analysis of the obtained PCR products was performed using capillary electrophoresis (ABI Prism 3100 Genetic Analyzer). The method was employed to determine Y microdeletions in azoospermic (n = 49) and severe oligozoospermic (n = 149) men. The number of PCR cycle (from 45 to 30) and the amount of DNA template (20-fold) used in fluorescent multiplex PCR were reduced because of the high sensitivity of capillary electrophoresis. Approximately 1000 multiplex PCR sets from 198 patients were analyzed simultaneously within 50 h. Y microdeletions were found in 3 out of the 198 azoospermic or severe oligozoosp...","downloadable_attachments":[{"id":39795447,"asset_id":17950764,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37868634,"first_name":"Pinar","last_name":"Bor","domain_name":"independent","page_name":"PinarBor","display_name":"Pinar Bor","profile_url":"https://independent.academia.edu/PinarBor?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=15375","nofollow":false},{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=15375","nofollow":false},{"id":178928,"name":"Male Infertility","url":"https://www.academia.edu/Documents/in/Male_Infertility?f_ri=15375","nofollow":false},{"id":191520,"name":"multiplex PCR","url":"https://www.academia.edu/Documents/in/multiplex_PCR?f_ri=15375"},{"id":1863536,"name":"Assisted","url":"https://www.academia.edu/Documents/in/Assisted?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_4687292" data-work_id="4687292" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/4687292/Determination_of_arsenic_species_by_field_amplified_injection_capillary_electrophoresis_after_modification_of_the_sample_solution_with_methanol">Determination of arsenic species by field amplified injection capillary electrophoresis after modification of the sample solution with methanol</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4687292" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b71e4c9261aba04e5444341e834d1a19" rel="nofollow" data-download="{"attachment_id":37113197,"asset_id":4687292,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/37113197/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="5961249" href="https://unex.academia.edu/EduardoPinillaGil">Eduardo Pinilla-Gil</a><script data-card-contents-for-user="5961249" type="text/json">{"id":5961249,"first_name":"Eduardo","last_name":"Pinilla-Gil","domain_name":"unex","page_name":"EduardoPinillaGil","display_name":"Eduardo 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$(".js-percentile-work_4687292"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_4687292 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="4687292"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 4687292; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=4687292]").text(description); $(".js-view-count-work_4687292").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_4687292").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" 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Arsenic","url":"https://www.academia.edu/Documents/in/Quantification_and_speciation_of_Arsenic?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=4687292]'), work: {"id":4687292,"title":"Determination of arsenic species by field amplified injection capillary electrophoresis after modification of the sample solution with methanol","created_at":"2013-10-05T17:15:03.806-07:00","url":"https://www.academia.edu/4687292/Determination_of_arsenic_species_by_field_amplified_injection_capillary_electrophoresis_after_modification_of_the_sample_solution_with_methanol?f_ri=15375","dom_id":"work_4687292","summary":null,"downloadable_attachments":[{"id":37113197,"asset_id":4687292,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":5961249,"first_name":"Eduardo","last_name":"Pinilla-Gil","domain_name":"unex","page_name":"EduardoPinillaGil","display_name":"Eduardo 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u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The cytolytic seed protein enterolobin from seeds ofEnterolobium contortisiliquumwas purified by using FPLC on a Mono Q column giving a single peak in capillary electrophoresis. The complete amino acid sequence of the plant cytolysin was... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13114307" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The cytolytic seed protein enterolobin from seeds ofEnterolobium contortisiliquumwas purified by using FPLC on a Mono Q column giving a single peak in capillary electrophoresis. The complete amino acid sequence of the plant cytolysin was determined by an automated method, yielding a molecular mass of 54,806 Da. Databank searches and sequence alignment demonstrated a high degree of sequence identity and</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13114307" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8536018c122899f458a1e94a836798d8" rel="nofollow" data-download="{"attachment_id":45655160,"asset_id":13114307,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45655160/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32364467" href="https://brasilia.academia.edu/WagnerFontes">Wagner Fontes</a><script data-card-contents-for-user="32364467" type="text/json">{"id":32364467,"first_name":"Wagner","last_name":"Fontes","domain_name":"brasilia","page_name":"WagnerFontes","display_name":"Wagner Fontes","profile_url":"https://brasilia.academia.edu/WagnerFontes?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_13114307 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13114307"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13114307, container: ".js-paper-rank-work_13114307", }); 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The complete amino acid sequence of the plant cytolysin was determined by an automated method, yielding a molecular mass of 54,806 Da. Databank searches and sequence alignment demonstrated a high degree of sequence identity and","downloadable_attachments":[{"id":45655160,"asset_id":13114307,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32364467,"first_name":"Wagner","last_name":"Fontes","domain_name":"brasilia","page_name":"WagnerFontes","display_name":"Wagner Fontes","profile_url":"https://brasilia.academia.edu/WagnerFontes?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4512,"name":"Light Scattering","url":"https://www.academia.edu/Documents/in/Light_Scattering?f_ri=15375","nofollow":false},{"id":4600,"name":"Protein Structure Prediction","url":"https://www.academia.edu/Documents/in/Protein_Structure_Prediction?f_ri=15375","nofollow":false},{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":29082,"name":"Sequence Analysis","url":"https://www.academia.edu/Documents/in/Sequence_Analysis?f_ri=15375","nofollow":false},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment?f_ri=15375"},{"id":76407,"name":"Circular Dichroism","url":"https://www.academia.edu/Documents/in/Circular_Dichroism?f_ri=15375"},{"id":81067,"name":"Bacterial Toxins","url":"https://www.academia.edu/Documents/in/Bacterial_Toxins?f_ri=15375"},{"id":128057,"name":"Light","url":"https://www.academia.edu/Documents/in/Light?f_ri=15375"},{"id":176238,"name":"Trees","url":"https://www.academia.edu/Documents/in/Trees?f_ri=15375"},{"id":295928,"name":"Amino Acids","url":"https://www.academia.edu/Documents/in/Amino_Acids?f_ri=15375"},{"id":372917,"name":"Protein Secondary Structure Prediction","url":"https://www.academia.edu/Documents/in/Protein_Secondary_Structure_Prediction?f_ri=15375"},{"id":386438,"name":"Seeds","url":"https://www.academia.edu/Documents/in/Seeds?f_ri=15375"},{"id":585595,"name":"Molecular Mass","url":"https://www.academia.edu/Documents/in/Molecular_Mass?f_ri=15375"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=15375"},{"id":1181939,"name":"PLANT PROTEINS","url":"https://www.academia.edu/Documents/in/PLANT_PROTEINS?f_ri=15375"},{"id":1451703,"name":"Cytotoxins","url":"https://www.academia.edu/Documents/in/Cytotoxins?f_ri=15375"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=15375"},{"id":2391248,"name":"Cytolysin","url":"https://www.academia.edu/Documents/in/Cytolysin?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1815201" data-work_id="1815201" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1815201/Development_and_Validation_of_a_Stability_Indicating_HPLC_Assay_Method_for_Simultaneous_Determination_of_Spironolactone_and_Furosemide_in_Tablet_Formulation">Development and Validation of a Stability-Indicating HPLC Assay Method for Simultaneous Determination of Spironolactone and Furosemide in Tablet Formulation</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The objective of the current study was to develop and validate a simple, precise and accurate isocratic stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) assay method for the determination of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_1815201" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The objective of the current study was to develop and validate a simple, precise and accurate isocratic stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) assay method for the determination of spironolactone and furosemide in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on an SGE 150 × 4.6 mm SS Wakosil II 5C8RS 5-μm column using a mobile phase of acetonitrile-ammonium acetate buffer (50:50, v/v) at a flow rate of 1.0 mL/min. The detection was carried out at 254 nm using a photodiode array detector. The drug was subject to oxidation, hydrolysis, photolysis and heat to apply stress conditions. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was found to be linear in the drug concentration range of 40-160 µg/mL with correlation coefficients of 0.9977 and 0.9953 for spironolactone and furosemide, respectively. The precision (relative standard deviation; RSD) among a six-sample preparation was 0.87% and 1.1% for spironolactone and furosemide, respectively. Repeatability and intermediate precision (RSD) among a six-sample preparation were 0.46% and 0.20% for spironolactone and furosemide, respectively. The accuracy (recovery) was between 98.05 and 100.17% and 99.07 and 100.58% for spironolactone and furosemide, respectively. Degradation products produced as a result of stress studies did not interfere with the detection of spironolactone and furosemide; therefore, the assay can be considered to be stability-indicating.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/1815201" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="859e0765d8ef2c5565a0cf206b84096a" rel="nofollow" data-download="{"attachment_id":26421983,"asset_id":1815201,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/26421983/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="2199776" href="https://digitaluniversity.academia.edu/vijayram">vijay ram</a><script data-card-contents-for-user="2199776" type="text/json">{"id":2199776,"first_name":"vijay","last_name":"ram","domain_name":"digitaluniversity","page_name":"vijayram","display_name":"vijay ram","profile_url":"https://digitaluniversity.academia.edu/vijayram?f_ri=15375","photo":"https://0.academia-photos.com/2199776/705537/876326/s65_vijay.ram.jpg"}</script></span></span></li><li class="js-paper-rank-work_1815201 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="1815201"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 1815201, container: ".js-paper-rank-work_1815201", }); 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Isocratic RP-HPLC separation was achieved on an SGE 150 × 4.6 mm SS Wakosil II 5C8RS 5-μm column using a mobile phase of acetonitrile-ammonium acetate buffer (50:50, v/v) at a flow rate of 1.0 mL/min. The detection was carried out at 254 nm using a photodiode array detector. The drug was subject to oxidation, hydrolysis, photolysis and heat to apply stress conditions. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was found to be linear in the drug concentration range of 40-160 µg/mL with correlation coefficients of 0.9977 and 0.9953 for spironolactone and furosemide, respectively. The precision (relative standard deviation; RSD) among a six-sample preparation was 0.87% and 1.1% for spironolactone and furosemide, respectively. Repeatability and intermediate precision (RSD) among a six-sample preparation were 0.46% and 0.20% for spironolactone and furosemide, respectively. The accuracy (recovery) was between 98.05 and 100.17% and 99.07 and 100.58% for spironolactone and furosemide, respectively. 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u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19970653 coauthored" data-work_id="19970653" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19970653/Performance_of_a_sound_card_as_data_acquisition_system_and_a_lock_in_emulated_by_software_in_capillary_electrophoresis">Performance of a sound card as data acquisition system and a lock-in emulated by software in capillary electrophoresis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The performance of fluorescence detectors in capillary electrophoresis is maximized when the excitation light intensity is modulated in time with optimal frequencies. This is especially true when photomultiplier tubes are used to detect... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19970653" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The performance of fluorescence detectors in capillary electrophoresis is maximized when the excitation light intensity is modulated in time with optimal frequencies. This is especially true when photomultiplier tubes are used to detect the fluorescent light. The photomultiplier tube amplified raw output signal can in principle be captured directly by a personal computer sound card (PCSC) and processed by a lock-in emulated by software. This possibility is demonstrated in the present work and the performance of this new setup is compared with a traditional data acquisition system. The results obtained with this &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;PCSC and lock-in emulated by software&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; were of the same quality or even better compared to that obtained by conventional time integrators (Boxcars) and data acquisition boards. With PCSC the limits of detection (LOD) found for both naphthalene-2,3-dicarboxaldehyde-derivatized tyrosine and alanine were 3.3 and 3.5fmol (injection of 5nL of samples at 0.66 and 0.70micromol/L), respectively. This is at least three times better compared to conventional systems when light emitting diodes (LEDs) are used as the excitation source in fluorescence detectors. The PCSC linear response range was also larger compared to conventional data acquisition boards. This scheme showed to be a practical and convenient alternative of data acquisition and signal processing for detection systems used in capillary electrophoresis.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19970653" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2be1eece6ef738ac581594976c3a91e2" rel="nofollow" data-download="{"attachment_id":41999160,"asset_id":19970653,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41999160/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="40846723" href="https://ufrgs.academia.edu/TarsoKist">Tarso Kist</a><script data-card-contents-for-user="40846723" type="text/json">{"id":40846723,"first_name":"Tarso","last_name":"Kist","domain_name":"ufrgs","page_name":"TarsoKist","display_name":"Tarso Kist","profile_url":"https://ufrgs.academia.edu/TarsoKist?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-19970653">+1</span><div class="hidden js-additional-users-19970653"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/ReginaldoRego">Reginaldo Rego</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-19970653'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-19970653').html(); 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This is especially true when photomultiplier tubes are used to detect the fluorescent light. The photomultiplier tube amplified raw output signal can in principle be captured directly by a personal computer sound card (PCSC) and processed by a lock-in emulated by software. This possibility is demonstrated in the present work and the performance of this new setup is compared with a traditional data acquisition system. The results obtained with this \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;PCSC and lock-in emulated by software\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; were of the same quality or even better compared to that obtained by conventional time integrators (Boxcars) and data acquisition boards. With PCSC the limits of detection (LOD) found for both naphthalene-2,3-dicarboxaldehyde-derivatized tyrosine and alanine were 3.3 and 3.5fmol (injection of 5nL of samples at 0.66 and 0.70micromol/L), respectively. This is at least three times better compared to conventional systems when light emitting diodes (LEDs) are used as the excitation source in fluorescence detectors. The PCSC linear response range was also larger compared to conventional data acquisition boards. This scheme showed to be a practical and convenient alternative of data acquisition and signal processing for detection systems used in capillary electrophoresis.","downloadable_attachments":[{"id":41999160,"asset_id":19970653,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":40846723,"first_name":"Tarso","last_name":"Kist","domain_name":"ufrgs","page_name":"TarsoKist","display_name":"Tarso Kist","profile_url":"https://ufrgs.academia.edu/TarsoKist?f_ri=15375","photo":"/images/s65_no_pic.png"},{"id":41193378,"first_name":"Reginaldo","last_name":"Rego","domain_name":"independent","page_name":"ReginaldoRego","display_name":"Reginaldo Rego","profile_url":"https://independent.academia.edu/ReginaldoRego?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=15375","nofollow":false},{"id":2141,"name":"Signal 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Practices and Advances Applied to the Analysis of Common</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">In 1997, low-level perchlorate contamination (&lt;50 ng mL -1 or parts per billion) was discovered in the western U.S. Since that time, it has been found in sites scattered around the nation. Although the Environmental Protection Agency... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_52669187" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In 1997, low-level perchlorate contamination (&lt;50 ng mL -1 or parts per billion) was discovered in the western U.S. Since that time, it has been found in sites scattered around the nation. Although the Environmental Protection Agency has not established a regulation for perchlorate in drinking water, it has placed perchlorate on the contaminant candidate list (CCL) and the unregulated</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/52669187" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4d0be3ef0c985c77d4cfb083500d364b" rel="nofollow" data-download="{"attachment_id":69823104,"asset_id":52669187,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/69823104/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="202183082" href="https://independent.academia.edu/EdwardUrbansky">Edward Urbansky</a><script data-card-contents-for-user="202183082" type="text/json">{"id":202183082,"first_name":"Edward","last_name":"Urbansky","domain_name":"independent","page_name":"EdwardUrbansky","display_name":"Edward Urbansky","profile_url":"https://independent.academia.edu/EdwardUrbansky?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_52669187 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="52669187"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 52669187, container: ".js-paper-rank-work_52669187", }); 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href="https://www.academia.edu/3195085/Development_and_application_of_a_T_RFLP_data_analysis_method_using_correlation_coefficient_matrices">Development and application of a T-RFLP data analysis method using correlation coefficient matrices</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Environmental microbiology studies commonly use terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes, for example, to analyze changes in community structure in relation to changing physicochemical and biological... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_3195085" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Environmental microbiology studies commonly use terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes, for example, to analyze changes in community structure in relation to changing physicochemical and biological conditions over space and time. Although T-RFLP is most useful for comparing samples from different environments, a large number of samples makes effective analysis difficult using the Web-based tools that are currently available. To resolve this dilemma, we used a new approach for calculating data from multiple T-RFLP samples by estimating terminal fragment combinations, then applying a correlation analysis using two different fluorescent dyes generated simultaneously from all samples. This calculation was based on the expectation that the proportions of two terminal fragments from one full-length polymerase chain reaction fragment would be nearly the same in each analysis. Using this program, the oral microflora in 73 human saliva samples were analyzed, and 24 bacterial groups, with peak areas of at least 0.5% and correlation coefficients of 0.55 or greater, were identified from the T-RFs within 40 s.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3195085" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="da32e40c36d26d2247e11ce01f5404e4" rel="nofollow" data-download="{"attachment_id":50417707,"asset_id":3195085,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50417707/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="2688511" href="https://nihon-u.academia.edu/nkamio">noriaki kamio</a><script data-card-contents-for-user="2688511" type="text/json">{"id":2688511,"first_name":"noriaki","last_name":"kamio","domain_name":"nihon-u","page_name":"nkamio","display_name":"noriaki kamio","profile_url":"https://nihon-u.academia.edu/nkamio?f_ri=15375","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_3195085 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="3195085"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 3195085, container: ".js-paper-rank-work_3195085", }); 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Although T-RFLP is most useful for comparing samples from different environments, a large number of samples makes effective analysis difficult using the Web-based tools that are currently available. To resolve this dilemma, we used a new approach for calculating data from multiple T-RFLP samples by estimating terminal fragment combinations, then applying a correlation analysis using two different fluorescent dyes generated simultaneously from all samples. This calculation was based on the expectation that the proportions of two terminal fragments from one full-length polymerase chain reaction fragment would be nearly the same in each analysis. Using this program, the oral microflora in 73 human saliva samples were analyzed, and 24 bacterial groups, with peak areas of at least 0.5% and correlation coefficients of 0.55 or greater, were identified from the T-RFs within 40 s.","downloadable_attachments":[{"id":50417707,"asset_id":3195085,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":2688511,"first_name":"noriaki","last_name":"kamio","domain_name":"nihon-u","page_name":"nkamio","display_name":"noriaki kamio","profile_url":"https://nihon-u.academia.edu/nkamio?f_ri=15375","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=15375","nofollow":false},{"id":3701,"name":"RNA","url":"https://www.academia.edu/Documents/in/RNA?f_ri=15375","nofollow":false},{"id":6947,"name":"Medical 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fMRI","url":"https://www.academia.edu/Documents/in/data_analysis_methods_in_fMRI?f_ri=15375"},{"id":410595,"name":"Correlation Analysis","url":"https://www.academia.edu/Documents/in/Correlation_Analysis?f_ri=15375"},{"id":611814,"name":"Correlation coefficient","url":"https://www.academia.edu/Documents/in/Correlation_coefficient?f_ri=15375"},{"id":1556410,"name":"Full Length Movies","url":"https://www.academia.edu/Documents/in/Full_Length_Movies?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6815436" data-work_id="6815436" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" 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Different recombinant proteins... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_35448139" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In this work, a high-resolution CGE method for quantification and purity determination of recombinant proteins was developed, involving a single-component inclusion bodies (IBs) solubilization solution. Different recombinant proteins expressed as IBs were used to show method capabilities, using recombinant interferon-1b as the model protein for method validation. Method linearity was verified in the range from 0.05 to 0.40 mg/mL and a determination coefficient (r 2) of 0.99 was obtained. The LOQs and LODs were 0.018 and 0.006 mg/mL, respectively. RSD for protein content repeatability test was 2.29%. In addition, RSD for protein purity repeatability test was 4.24%. Method accuracy was higher than 90%. Specificity was confirmed, as the method was able to separate recombinant interferon-1b monomer from other aggregates and impurities. Sample content and purity was demonstrated to be stable for up to 48 h. Overall, this method is suitable for the analysis of recombinant proteins in IBs according to the attributes established on the International Conference for Harmonization guidelines.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/35448139" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="57ec896e097bad55818c53ccf852632f" rel="nofollow" data-download="{"attachment_id":55309591,"asset_id":35448139,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/55309591/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="72791781" href="https://independent.academia.edu/EmilioMedinaRivero">Emilio Medina-Rivero</a><script data-card-contents-for-user="72791781" type="text/json">{"id":72791781,"first_name":"Emilio","last_name":"Medina-Rivero","domain_name":"independent","page_name":"EmilioMedinaRivero","display_name":"Emilio Medina-Rivero","profile_url":"https://independent.academia.edu/EmilioMedinaRivero?f_ri=15375","photo":"https://0.academia-photos.com/72791781/18625281/18588283/s65_emilio.medina-rivero.jpg"}</script></span></span></li><li class="js-paper-rank-work_35448139 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="35448139"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 35448139, container: ".js-paper-rank-work_35448139", }); 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$(".js-view-count[data-work-id=35448139]").text(description); $(".js-view-count-work_35448139").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_35448139").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="35448139"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">2</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="990417" href="https://www.academia.edu/Documents/in/Recombinant_Proteins">Recombinant Proteins</a><script data-card-contents-for-ri="990417" type="text/json">{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=35448139]'), work: {"id":35448139,"title":"Capillary gel electrophoresis for the quantification and purity determination of recombinant proteins in inclusion bodies","created_at":"2017-12-16T23:03:44.534-08:00","url":"https://www.academia.edu/35448139/Capillary_gel_electrophoresis_for_the_quantification_and_purity_determination_of_recombinant_proteins_in_inclusion_bodies?f_ri=15375","dom_id":"work_35448139","summary":"In this work, a high-resolution CGE method for quantification and purity determination of recombinant proteins was developed, involving a single-component inclusion bodies (IBs) solubilization solution. Different recombinant proteins expressed as IBs were used to show method capabilities, using recombinant interferon-1b as the model protein for method validation. Method linearity was verified in the range from 0.05 to 0.40 mg/mL and a determination coefficient (r 2) of 0.99 was obtained. The LOQs and LODs were 0.018 and 0.006 mg/mL, respectively. RSD for protein content repeatability test was 2.29%. In addition, RSD for protein purity repeatability test was 4.24%. Method accuracy was higher than 90%. Specificity was confirmed, as the method was able to separate recombinant interferon-1b monomer from other aggregates and impurities. Sample content and purity was demonstrated to be stable for up to 48 h. Overall, this method is suitable for the analysis of recombinant proteins in IBs according to the attributes established on the International Conference for Harmonization guidelines.","downloadable_attachments":[{"id":55309591,"asset_id":35448139,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":72791781,"first_name":"Emilio","last_name":"Medina-Rivero","domain_name":"independent","page_name":"EmilioMedinaRivero","display_name":"Emilio Medina-Rivero","profile_url":"https://independent.academia.edu/EmilioMedinaRivero?f_ri=15375","photo":"https://0.academia-photos.com/72791781/18625281/18588283/s65_emilio.medina-rivero.jpg"}],"research_interests":[{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins?f_ri=15375","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_32038694" data-work_id="32038694" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/32038694/Enhancement_of_native_fluorescence_intensity_of_berberine_by_2_hydroxypropyl_%CE%B2_cyclodextrin_in_capillary_electrophoresis_coupled_by_laser_induced_fluorescence_detection_Application_to_quality_control_of_medicinal_plants">Enhancement of native fluorescence intensity of berberine by (2-hydroxypropyl)-β-cyclodextrin in capillary electrophoresis coupled by laser-induced fluorescence detection: Application to quality control of medicinal plants</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/32038694" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f8d71e71e10f8f00be2af8f9ec6a3118" rel="nofollow" data-download="{"attachment_id":52300803,"asset_id":32038694,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/52300803/download_file?st=MTczMjczMjg3Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32862349" href="https://istanbultek.academia.edu/FErim">F. 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Bedia Erim","profile_url":"https://istanbultek.academia.edu/FErim?f_ri=15375","photo":"https://0.academia-photos.com/32862349/16109521/16564710/s65_f._bedia.erim.jpg"}</script></span></span></li><li class="js-paper-rank-work_32038694 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="32038694"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 32038694, container: ".js-paper-rank-work_32038694", }); });</script></li><li class="js-percentile-work_32038694 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 32038694; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_32038694"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_32038694 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="32038694"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 32038694; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=32038694]").text(description); $(".js-view-count-work_32038694").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_32038694").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="32038694"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="923" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="15019" href="https://www.academia.edu/Documents/in/Medicinal_Plants">Medicinal Plants</a>, <script data-card-contents-for-ri="15019" type="text/json">{"id":15019,"name":"Medicinal Plants","url":"https://www.academia.edu/Documents/in/Medicinal_Plants?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a><script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=32038694]'), work: {"id":32038694,"title":"Enhancement of native fluorescence intensity of berberine by (2-hydroxypropyl)-β-cyclodextrin in capillary electrophoresis coupled by laser-induced fluorescence detection: Application to quality control of medicinal plants","created_at":"2017-03-25T05:36:46.921-07:00","url":"https://www.academia.edu/32038694/Enhancement_of_native_fluorescence_intensity_of_berberine_by_2_hydroxypropyl_%CE%B2_cyclodextrin_in_capillary_electrophoresis_coupled_by_laser_induced_fluorescence_detection_Application_to_quality_control_of_medicinal_plants?f_ri=15375","dom_id":"work_32038694","summary":null,"downloadable_attachments":[{"id":52300803,"asset_id":32038694,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32862349,"first_name":"F. Bedia","last_name":"Erim","domain_name":"istanbultek","page_name":"FErim","display_name":"F. Bedia Erim","profile_url":"https://istanbultek.academia.edu/FErim?f_ri=15375","photo":"https://0.academia-photos.com/32862349/16109521/16564710/s65_f._bedia.erim.jpg"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=15375","nofollow":false},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=15375","nofollow":false},{"id":15019,"name":"Medicinal Plants","url":"https://www.academia.edu/Documents/in/Medicinal_Plants?f_ri=15375","nofollow":false},{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":37306,"name":"Quality Control","url":"https://www.academia.edu/Documents/in/Quality_Control?f_ri=15375"},{"id":106562,"name":"Plant","url":"https://www.academia.edu/Documents/in/Plant?f_ri=15375"},{"id":234210,"name":"Berberine","url":"https://www.academia.edu/Documents/in/Berberine?f_ri=15375"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=15375"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=15375"},{"id":476883,"name":"Tablets","url":"https://www.academia.edu/Documents/in/Tablets?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27520354" data-work_id="27520354" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27520354/Analysis_of_biologically_active_amines_by_CE">Analysis of biologically active amines by CE</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27520354" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="32bbe0d87d66909d709c190e84de1450" rel="nofollow" data-download="{"attachment_id":47776017,"asset_id":27520354,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button 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Lin","profile_url":"https://ncue.academia.edu/YangWeiLin?f_ri=15375","photo":"https://0.academia-photos.com/51680604/17469190/17548167/s65_yang-wei.lin.jpg"}</script></span></span></li><li class="js-paper-rank-work_27520354 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="27520354"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 27520354, container: ".js-paper-rank-work_27520354", }); });</script></li><li class="js-percentile-work_27520354 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27520354; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = 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class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="371425" href="https://www.academia.edu/Documents/in/Electrophoresis">Electrophoresis</a><script data-card-contents-for-ri="371425" type="text/json">{"id":371425,"name":"Electrophoresis","url":"https://www.academia.edu/Documents/in/Electrophoresis?f_ri=15375","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=27520354]'), work: {"id":27520354,"title":"Analysis of biologically active amines by CE","created_at":"2016-08-03T22:48:48.531-07:00","url":"https://www.academia.edu/27520354/Analysis_of_biologically_active_amines_by_CE?f_ri=15375","dom_id":"work_27520354","summary":null,"downloadable_attachments":[{"id":47776017,"asset_id":27520354,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":51680604,"first_name":"Yang-Wei","last_name":"Lin","domain_name":"ncue","page_name":"YangWeiLin","display_name":"Yang-Wei Lin","profile_url":"https://ncue.academia.edu/YangWeiLin?f_ri=15375","photo":"https://0.academia-photos.com/51680604/17469190/17548167/s65_yang-wei.lin.jpg"}],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=15375","nofollow":false},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=15375","nofollow":false},{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=15375","nofollow":false},{"id":371425,"name":"Electrophoresis","url":"https://www.academia.edu/Documents/in/Electrophoresis?f_ri=15375","nofollow":false},{"id":783432,"name":"Biological activity","url":"https://www.academia.edu/Documents/in/Biological_activity?f_ri=15375"},{"id":872101,"name":"Biogenic amines","url":"https://www.academia.edu/Documents/in/Biogenic_amines?f_ri=15375"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=15375"},{"id":1957004,"name":"Stacking","url":"https://www.academia.edu/Documents/in/Stacking?f_ri=15375"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13267090" data-work_id="13267090" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13267090/Determination_of_mono_and_disaccharides_by_capillary_electrophoresis_with_contactless_conductivity_detection">Determination of mono- and disaccharides by capillary electrophoresis with contactless conductivity detection</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13267090" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="dc185b4c74526606d381b17e9cdf156f" rel="nofollow" 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