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class="woocommerce-notices-wrapper"></div> <header class="section-header"> <h1 class="product_title entry-title">ExpressPlex™ 2.0 Library Prep Kit – Early Access</h1> </header> <div class="pricing"> <p class="price"></p> </div> <div class="Request-Quote"> <a class="single_add_to_cart_button button alt" href="https://seqwell.com/request-quote/">Request Quote</a> </div> <div class="content-wrap"> <div class="woocommerce-product-details__short-description"> <p>ExpressPlex 2.0<strong><em> Early Access</em></strong>: The <strong><em>workflow simplicity</em></strong> of one-step library prep combined with the <strong><em>performance</em></strong> of TnX for high throughput sequencing of plasmids, amplicons and other synthetic constructs.</p> <ul> <li>Improved performance: <strong>TnX</strong> transposase for reduced bias and improved coverage uniformity</li> <li>Ease of use: Fragmentation, barcoding, and amplification in a single step</li> <li>Rapid: 100-minute workflow (30 minutes or less hands-on) with pre-plated, reaction-ready reagents</li> <li>Scalable: High-level sample multiplexing and auto normalization</li> </ul> </div> <a class="underline-link" target="_BLANK" href="https://seqwell.com/wp-content/uploads/2024/03/seqWell-Kit-Comparison-Guide.png">View Product Comparison Chart</a> </div> </div> </div> </header><section class="content-section white" id="content1"> <div class="contain wide"> <div class="content-wrap"> <h3 style="text-align: center;"><strong>We’ve unleashed TnX, our next generation transposase, in ExpressPlex 2.0 – a library prep kit that changes your NGS synthetic construct sequencing workflow forever!</strong></h3> <p>ExpressPlex 2.0 is an <strong><em>automation-friendly</em></strong>, <strong><em>one-step library prep</em></strong> powered by TnX to <strong><em>reduce bias</em></strong> and <strong><em>improve coverage uniformity</em></strong> using a highly <strong><em>streamlined workflow </em></strong>for sequencing plasmids, amplicons and other synthetic constructs.</p> </div> </div> <div class="contain wide"> <div class="col-3-5 content-wrap"> <p><img loading="lazy" decoding="async" class="aligncenter wp-image-5990 size-full" src="https://seqwell.com/wp-content/uploads/2024/03/Benefits.png" alt="" width="948" height="467" srcset="https://seqwell.com/wp-content/uploads/2024/03/Benefits.png 948w, https://seqwell.com/wp-content/uploads/2024/03/Benefits-300x148.png 300w, https://seqwell.com/wp-content/uploads/2024/03/Benefits-768x378.png 768w, https://seqwell.com/wp-content/uploads/2024/03/Benefits-600x296.png 600w" sizes="(max-width: 948px) 100vw, 948px" /></p> </div> <div class="col-2-5 content-wrap right"> <p><a href="https://seqwell.com/wp-content/uploads/2024/08/ExpressPlex2.0-Next-Generation-NGS-Library-Prep.pdf"><img loading="lazy" decoding="async" class="alignnone wp-image-6021 size-large" src="https://seqwell.com/wp-content/uploads/2024/09/24-sw-ep-2_0-infographic-teaser-c3-v2a-1024x759.jpg" alt="" width="1024" height="759" srcset="https://seqwell.com/wp-content/uploads/2024/09/24-sw-ep-2_0-infographic-teaser-c3-v2a-1024x759.jpg 1024w, https://seqwell.com/wp-content/uploads/2024/09/24-sw-ep-2_0-infographic-teaser-c3-v2a-300x222.jpg 300w, https://seqwell.com/wp-content/uploads/2024/09/24-sw-ep-2_0-infographic-teaser-c3-v2a-768x569.jpg 768w, https://seqwell.com/wp-content/uploads/2024/09/24-sw-ep-2_0-infographic-teaser-c3-v2a-600x445.jpg 600w, https://seqwell.com/wp-content/uploads/2024/09/24-sw-ep-2_0-infographic-teaser-c3-v2a.jpg 1160w" sizes="(max-width: 1024px) 100vw, 1024px" /></a></p> </div> </div> </section> <section class="content-section gray" id="content2"> <div class="contain wide"> <div class="content-wrap"> <h2 style="text-align: center;">ExpressPlex 2.0 One-step Library Prep</h2> <p><img loading="lazy" decoding="async" class="aligncenter wp-image-5989 size-large" src="https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex2_0-Workflow-e1724850850401-1024x664.png" alt="" width="1024" height="664" srcset="https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex2_0-Workflow-e1724850850401-1024x664.png 1024w, https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex2_0-Workflow-e1724850850401-300x195.png 300w, https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex2_0-Workflow-e1724850850401-768x498.png 768w, https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex2_0-Workflow-e1724850850401-1536x996.png 1536w, https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex2_0-Workflow-e1724850850401-2048x1329.png 2048w, https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex2_0-Workflow-e1724850850401-600x389.png 600w" sizes="(max-width: 1024px) 100vw, 1024px" /></p> </div> </div> </section> <section class="content-section blue image-texture" id="content3"> <div class="contain wide"> <div class="content-wrap"> <h3 style="text-align: center;"><span style="color: #ffffff;">“Anyone can rapidly produce a quality NGS library using ExpressPlex 2.0!”</span></h3> <p style="text-align: center;"><span style="color: #00ffff;">– Zachary Neuschaefer, Principal NGS Engineer, <em>LifeMine Therapeutics</em></span></p> </div> </div> </section> <section class="content-section white" id="content4"> <div class="contain "> <div class="content-wrap"> <h2 class="p1" style="text-align: center;">TnX Transposase: Quality Libraries for Quality Sequencing</h2> <p style="text-align: center;">ExpressPlex 2.0 uses tagmentation powered by TnX, the next generation transposase, to simultaneously fragment & index sample DNA. Average fragment size ranges from 400-1200 bp.</p> <p style="text-align: center;"><img loading="lazy" decoding="async" class="aligncenter wp-image-5991 size-full" src="https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex-2-Fragment-Profile.png" alt="" width="719" height="297" srcset="https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex-2-Fragment-Profile.png 719w, https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex-2-Fragment-Profile-300x124.png 300w, https://seqwell.com/wp-content/uploads/2024/03/ExpressPlex-2-Fragment-Profile-600x248.png 600w" sizes="(max-width: 719px) 100vw, 719px" /></p> <h6 style="text-align: center;">Typical fragment profile following analysis on an Agilent TapeStation System. Data shown are for a 96-plex sample.</h6> </div> </div> <div class="contain "> <div class="content-wrap"> <h2></h2> <h2 class="p1" style="text-align: center;">TnX: Transposase Engineered for Reduced Insertion Bias</h2> <h3 class="p1" style="text-align: center;">Better transposase = better sequencing. Take your plasmid and amplicon screening to the next level!</h3> <p style="text-align: center;">ExpressPlex 2.0 is the only library prep kit powered by TnX! TnX was engineered to reduce insertional bias to improve uniformity of coverage. When directly compared to a Tn5-based competitor library prep kit for sequencing of 2 different plasmids, ExpressPlex 2.0 produced <strong>significantly lower CVs and range of coverage (max/min).</strong></p> <p> </p> <p style="text-align: center;"><img loading="lazy" decoding="async" class="aligncenter wp-image-5992 size-full" src="https://seqwell.com/wp-content/uploads/2024/03/TnX-Reduced-Bias.png" alt="" width="960" height="537" srcset="https://seqwell.com/wp-content/uploads/2024/03/TnX-Reduced-Bias.png 960w, https://seqwell.com/wp-content/uploads/2024/03/TnX-Reduced-Bias-300x168.png 300w, https://seqwell.com/wp-content/uploads/2024/03/TnX-Reduced-Bias-768x430.png 768w, https://seqwell.com/wp-content/uploads/2024/03/TnX-Reduced-Bias-600x336.png 600w" sizes="(max-width: 960px) 100vw, 960px" /></p> <h6 style="text-align: center;">Library preparation for pUC19 and pMal-c67 was conducted using ExpressPlex 2.0 kit and a Tn5-based competitor kit using standard manufacturers’ protocols. Libraries were sequenced using an Illumina MiSeq.</h6> <p style="text-align: center;"> </div> </div> </section> <section class="content-section blue image-texture" id="content5"> <div class="contain full flex"> <div class="col-1-4 content-wrap"> <h1 style="text-align: right;"><img loading="lazy" decoding="async" class="alignright wp-image-5997 size-full" src="https://seqwell.com/wp-content/uploads/2024/03/4_4-stat-1.png" alt="" width="1843" height="1158" srcset="https://seqwell.com/wp-content/uploads/2024/03/4_4-stat-1.png 1843w, https://seqwell.com/wp-content/uploads/2024/03/4_4-stat-1-300x188.png 300w, https://seqwell.com/wp-content/uploads/2024/03/4_4-stat-1-1024x643.png 1024w, https://seqwell.com/wp-content/uploads/2024/03/4_4-stat-1-768x483.png 768w, https://seqwell.com/wp-content/uploads/2024/03/4_4-stat-1-1536x965.png 1536w, https://seqwell.com/wp-content/uploads/2024/03/4_4-stat-1-600x377.png 600w" sizes="(max-width: 1843px) 100vw, 1843px" /></h1> </div> <div class="col-3-4 content-wrap right"> <h2 style="text-align: left;"><span style="color: #ffffff;"><em>initial ExpressPlex 2.0 users said it exceeded their workflow, productivity & cost expectations!</em></span></h2> </div> </div> </section> <section class="content-section gray" id="content6"> <div class="contain wide"> <div class="image-wrap fade right in-left col-1-2"> <img src="https://seqwell.com/wp-content/uploads/2023/03/MAR-23-OCTANT-WEBINAR-VIDEO-THMB-V04-1200px-675px-Tighter-top-bar-1024x576-1.jpg" alt="" /> </div> <div class="content-wrap col-1-2"> <h2>Recorded Webinar | Octant Bio</h2> <p class="p1">The game-changing capabilities enabled by the ExpressPlex workflow were highlighted in a webinar collaboration with the synthetic biology team from Octant Bio, a drug discovery company located in Emeryville, CA. Octant’s Henry Chan, PhD (Synthetic Biology Lead) and Bryan Jiang (Research Associate) discussed the application of ExpressPlex to OCTOPUS, the high-throughput plasmid sequencing platform that powers Octant’s therapeutic discovery platform. Watch the video to discover how ExpressPlex has dramatically improved OCTOPUS and accelerated their overall discovery process.</p> <div></div> <p><a style="font-weight: bold; font-size: 16px; text-decoration: none; background-color: #6cc249; padding: 10px 18px; border-radius: 5px; color: white; margin-top: 10px;" href="https://seqwell.com/webinar-octant/" target="_blank" rel="noopener">Watch Now</a></p> </div> </div> </section> <section class="content-section white" id="content7"> <div class="contain wide"> <div class="content-wrap"> <h2 style="text-align: center;">Improving Sustainability Through Streamlined Protocols</h2> </div> </div> <div class="contain wide flex"> <div class="col-1-2 content-wrap"> <p><img loading="lazy" decoding="async" class="wp-image-5998 size-medium alignnone" src="https://seqwell.com/wp-content/uploads/2024/03/Think-Green-300x278.png" alt="" width="300" height="278" srcset="https://seqwell.com/wp-content/uploads/2024/03/Think-Green-300x278.png 300w, https://seqwell.com/wp-content/uploads/2024/03/Think-Green-600x555.png 600w, https://seqwell.com/wp-content/uploads/2024/03/Think-Green.png 617w" sizes="(max-width: 300px) 100vw, 300px" /></p> <p>The automation-friendly ExpressPlex one-step tagmentation workflow enables a <strong>>80%</strong> reduction in tips/plastics usage. We help drive sustainability not only in our internal sequencing services pipeline, but for our customers across the globe.</p> </div> <div class="col-1-2 content-wrap right"> <p>Listen to an interview with Jack Leonard, seqWell’s co-founder and CTO, as he discusses addressing increased sustainability within the world of synthetic biology on sptlabtech’s “labtech unplugged” podcast.</p> <p><a href="https://www.sptlabtech.com/labtechunplugged"><img loading="lazy" decoding="async" class="aligncenter wp-image-5999 size-thumbnail" src="https://seqwell.com/wp-content/uploads/2024/03/labtech-unplugged-150x150.png" alt="" width="150" height="150" srcset="https://seqwell.com/wp-content/uploads/2024/03/labtech-unplugged-150x150.png 150w, https://seqwell.com/wp-content/uploads/2024/03/labtech-unplugged-100x100.png 100w" sizes="(max-width: 150px) 100vw, 150px" /></a></p> <p> </p> <p> </p> </div> </div> </section> <section class="content-section white" id="content8"> <div class="contain narrow"> <div class="cta-block fade in-up"> <div class="flex"> <header class="content-wrap"> <h2>Are you in need of bulk-dispensed product for your miniaturized reactions?</h2> </header> <footer> <a class="button" href="https://seqwell.com/get-info-on-bulk-reagent/" target="_blank">Contact Us</a> </footer> </div> </div> </div> </section> <section class="content-section product-content"> <div class="contain wide"> <div class="col-1-2 content-wrap"> <h2>ExpressPlex 2.0 Specifications (Early Access)</h2> <table> <tbody> <tr> <td colspan="2"><span style="font-weight: 400;">ExpressPlex 2.0 Specifications</span></td> </tr> <tr> <td><span style="font-weight: 400;">Primary Applications</span></td> <td><span style="font-weight: 400;">Plasmid and amplicon sequencing</span></td> </tr> <tr> <td><span style="font-weight: 400;">Sample Input types</span></td> <td><span style="font-weight: 400;">Purified plasmid DNA, RCA-amplified DNA, amplicons >350bp, colony PCR amplicons</span></td> </tr> <tr> <td><span style="font-weight: 400;">Transposase</span></td> <td><span style="font-weight: 400;">TnX – Next generation engineered transposase</span></td> </tr> <tr> <td><span style="font-weight: 400;">Reactions per Kit</span></td> <td> <ul> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">96 </span></li> <li><span style="font-weight: 400;">384 (4 different sets available)</span></li> </ul> </td> </tr> <tr> <td><span style="font-weight: 400;">DNA Input Recommended</span></td> <td><span style="font-weight: 400;">1-40ng</span></td> </tr> <tr> <td><span style="font-weight: 400;">Total Library Prep Time</span></td> <td><span style="font-weight: 400;">100 minutes (30 minutes hands-on time)</span></td> </tr> <tr> <td><span style="font-weight: 400;">Indexing Method</span></td> <td><span style="font-weight: 400;">Combinatorial Dual Indexing</span></td> </tr> <tr> <td><span style="font-weight: 400;">Number of Unique Index Combinations</span></td> <td><span style="font-weight: 400;">1536</span></td> </tr> <tr> <td><span style="font-weight: 400;">Batch Size</span></td> <td><span style="font-weight: 400;">48-96 samples per batch</span></td> </tr> <tr> <td><span style="font-weight: 400;">Output Fragment Range*</span></td> <td><span style="font-weight: 400;">400 – 1,200bp</span></td> </tr> <tr> <td><span style="font-weight: 400;">Number of PCR Cycles</span></td> <td> <ul> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">12 cycles for plasmids</span></li> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">15 cycles for amplicons</span></li> </ul> </td> </tr> <tr> <td><span style="font-weight: 400;">Sequencer Compatibility</span></td> <td> <ul> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">All Illumina sequencing platforms</span></li> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">Compatible with Complete Genomics platforms or Element Biosciences AVITI™ using conversion kits for Illumina libraries</span></li> </ul> </td> </tr> </tbody> </table> <p><span style="font-weight: 400;">* Fragment size will depend on magnetic bead cleanup ratios used.</span></p> <h4 class="p1">ExpressPlex 2.0 Library Prep Kit Includes:</h4> <ul class="ul1"> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">Indexing Reaction Plate(s)</span></li> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">Ready Reaction Mix Plate(s)</span></li> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">MAGwise Paramagnetic Beads</span></li> <li style="font-weight: 400;" aria-level="1"><span style="font-weight: 400;">Users do not need to supply polymerase, primers, or barcodes. These are already pre-mixed into the reagents supplied.</span></li> </ul> </div> <div class="col-1-2"> <header class="section-header"> <h2>ExpressPlex™ 2.0 Library Prep Kit – Early Access Resources</h2> </header> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2024/08/ExpressPlex2.0-Next-Generation-NGS-Library-Prep.pdf" target="_blank" > <h4>Explore ExpressPlex 2.0 One-step Library Prep</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2024/08/seqWell_ExpressPlex-2_0-TS_EP2082224.pdf" target="_blank" > <h4>ExpressPlex 2.0 Technical Specification Sheet</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2024/08/seqWell_User_Guide_EP20EA20240818-1.pdf" target="_blank" > <h4>ExpressPlex 2.0 User Guide – Early Access</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2024/08/ExpressPlex-2-0-Master-Index-List_v20240816.xlsx" target="_blank" > <h4>ExpressPlex 2.0 Index List</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2024/08/20240820-XLEAP-guidelines.pdf" target="_blank" > <h4>XLEAP Recommended Guidelines</h4> </a> </div> </div> </section> <footer id="bottom"> <div class="contain wide flex"> <div class="col-1-4 content-wrap"> <h4>Office</h4> <p>66 Cherry Hill Drive<br /> Beverly, MA 01915<br /> 1 (855) SEQWELL (737-9355)</p> <p><script src="https://kit.fontawesome.com/0e0cfcdfba.js" crossorigin="anonymous"></script></p> <style> .footer-socials a { display: inline-block; 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