Custom Membrane Protein Production Services

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Due to their low natural abundance and highly complicated structure (partially or completely associated with plasma membrane), obtaining recombinant membrane protein products with native conformation and bio-activity has always been a challenging issue. </p> <p class="show-center"> <img loading="lazy" src="static/img/4-1-1-4-custom-membrane-protein-production-1.jpg" width="660" height="216" alt="Membrane Protein (Creative Biolabs Original)" /> </p> <h3> Expression Strategies of Membrane Protein Production </h3> <div class="table-responsive"> <table class="table-bordered table"> <tbody> <tr> <td> <strong><a href="/Cell-based-Membrane-Protein-Expression.html">Cell-based Membrane Protein Production</a> </strong> </td> <td> <strong><a href="/cell-free-membrane-protein-expression.html">Cell-free Membrane Protein Production</a> </strong> </td> </tr> <tr> <td> Cell-based Membrane Protein Expression and Purification is the most commonly used approach for membrane protein production. For membrane proteins, it can be problematic for adequate expression levels, correct membrane insertion, and native confirmation preservation. Using our proprietary vector designs and special protocols, we have successfully generated a broad spectrum of targets. </td> <td> Cell-free Membrane Protein Expression and Purification platform which is derived from modified cellular extracts, exerts unique beneficial features for expressing many challenging or "difficult" targets, especially membrane proteins or toxic proteins. This highly robust, efficient, and controllable system can also be directly reconstituted with lipids or nanodiscs, avoiding the use of detergent. </td> </tr> </tbody> </table> </div> <h3> Solubilization Strategies of Membrane Protein Production </h3> <div class="table-responsive"> <table class="table-bordered table"> <tbody> <tr> <td> <strong><a href="/magic-membrane-protein-solubilization-in-micelles.html">Membrane Protein Solubilization in Micelle</a> </strong> </td> <td> <strong><a href="/Mempro-Protein-Production-in-Liposomes.htm">Membrane Protein Reconstitution in Liposome</a> </strong> </td> </tr> <tr> <td> For the most part, distinct micelles must be utilized for various applications and the extraction of membrane proteins from various sources. Creative Biolabs can identify the optimal micelles with high purity, stability, and yield for our customers' unique membrane protein targets thanks to our extensive knowledge in this field and the variety of detergents and lipids we have available. </td> <td> Creative Biolabs offers a wide range of lipids that are synthesized or isolated from natural sources. To stabilize the proteoliposomes and maintain the function of the membrane proteins in their original form as much as possible, we can also dope them with the appropriate types and fractions of charged lipids, modified lipids, and other additives like cholesterols. </td> </tr> <tr> <td> <strong><a href="/Membrane-Protein-Purification-Using-Nanodiscs.htm">Membrane Protein Incorporation in Nanodisc</a> </strong> </td> <td> <strong><a href="/Membrane-Protein-Production-Using-Polymers-Service.htm">Membrane Protein Incorporation in Polymer</a> </strong> </td> </tr> <tr> <td> We will use detergents to solubilize and purify your membrane proteins in a micellar solution. Following the solubilization and purification, the detergents will be eliminated by binding hydrophobic beads or by dialysis. Lipids and the membrane scaffolding protein (MSP) will then be added to the micellar solution of membrane proteins. </td> <td> Using a range of techniques, Creative Biolabs has been committed to offering top-notch membrane protein synthesis services. The capacity to encapsulate a membrane protein in a more "natural" environment than that offered by detergents is the polymer system's most important advantage. </td> </tr> <tr> <td> <strong><a href="/magic-membrane-protein-incorporation-in-amphipols.html">Membrane Protein Incorporation in Amphipols</a></strong> </td> <td> <strong><a href="/membrane-protein-expression-in-vlps.html">Membrane Protein Expression in VLP</a></strong> </td> </tr> <tr> <td> Typically, detergents are used to remove membrane proteins, which are then moved to amphipol solutions. Compacts of amphipol-detergent will envelop membrane proteins. After that, detergent components can be eliminated by letting polystyrene beads incubate. </td> <td> Membrane Protein VLPs (also known as non-infectious virus-like particles) are characterized by the presence of highly expressed native conformations of particular membrane proteins. MP-VLPs can arise when VLPs sprout from host cells that overexpress a target membrane protein. </td> </tr> <tr> <td> <strong><a href="/virus-like-particles-for-membrane-protein-expression.html">Gag VLP based Membrane Protein Generation</a> </strong> </td> <td> <strong><a href="/Mempro-detergent-free-protein-production.htm">Detergent-free Membrane Protein Generation</a> </strong> </td> </tr> <tr> <td> To support our clients' various research objectives, Creative Biolabs has incorporated unique lipoparticle technology that can reconstitute the original target protein onto the recombinant VLP surface. </td> <td> Creative Biolabs provides an alternate method to maintain membrane protein solubility in water without the need for detergent. There are multiple methods we can use to produce membrane proteins without the need for detergents. </td> </tr> </tbody> </table> </div> <p> Equipped with multiple leading technologies and an abundance of expertise, <strong>Creative Biolabs</strong> is confident in delivering satisfactory membrane protein products with our first-class professional service. We provide quite flexible options for expression, solubilization/reconstitution, and purification as well as characterization (such as binding assays, and functional assays). A pilot study is strongly recommended to gather some preliminary data, and then we can move to further optimizations and the scaling-up stage. Please feel free to <a class="inquiry-button">contact us</a> for a detailed quote. </p><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.</p> <div class="related-sections"> <b>Related Sections</b> <b>Services</b> <ul class="related-sections-ul"> <li><a href="/magic-membrane-protein-solubilization-and-stabilization.html" >Custom Membrane Protein Solubilization and Stabilization Services</a></li> <li><a href="/membrane-protein-service.html" >Custom Membrane Protein Specific Antibody Discovery Services</a></li> <li><a href="/magic-membrane-protein-characterization.html" >Membrane Protein Characterization Methods Introduction</a></li> <li><a href="/magic-membrane-protein-extraction-from-cell-membranes.html" >Cell Membrane based Native Membrane Protein Extraction Service</a></li> <li><a href="/anti-membrane-protein-antibody-production-using-recombinant-proteins.html" >Anti-Membrane Protein Antibody Production Using Recombinant Proteins</a></li> <li><a href="/gpcr-preparation-as-immunogen.html" >GPCR Immunogen Preparation Service</a></li> <li><a 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