Pools d’oligonucléotides pour les cribles à haut débit

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lectures longues </a> </li> <li class="menu-item menu-item--expanded level-2-category-section" data-column-position=1 data-column-v-weight=1> <div class="level-2-category-section "> <a href="https://www.twistbioscience.com/fr/products/ngs/rna-seq-solutions" class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> Séquençage de l’ARN > </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="https://www.twistbioscience.com/fr/products/ngs/rna-sequencing-solutions?tab=rna-library-prep" class=""> Préparation de banque d’ARN </a> </li> <li class="menu-item"> <a href="https://www.twistbioscience.com/fr/products/ngs/rna-sequencing-solutions?tab=rna-exome" class=""> Exome d’ARN </a> </li> <li class="menu-item"> <a href="https://www.twistbioscience.com/fr/products/ngs/rna-sequencing-solutions?tab=rna-fusion" class=""> Fusion d’ARN </a> </li> </ul> </div> </li> <li class="menu-item menu-item--expanded level-2-category-section" data-column-position=3 data-column-v-weight=1> <div class="level-2-category-section "> <span class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> Réactifs et kits </span> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="/fr/products/ngs/reagent-kits/fast-hybridization-and-wash-kit" class=""> Kit Fast Hybridization and Wash </a> </li> <li class="menu-item"> <a href="/fr/products/ngs/reagent-kits/standard-hybridization-and-wash-kit" class=""> Kit réactifs d’hybridation standard </a> </li> <li class="menu-item"> <a href="/fr/products/ngs/reagent-kits/universal-blockers" class=""> Universal Blockers </a> </li> </ul> </div> </li> <li class="menu-item menu-item--expanded level-2-category-section" data-column-position=3 data-column-v-weight=1> <div class="level-2-category-section "> <span class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> Contrôles et normes </span> <ul class="menu menu-level-3"> <li class="menu-item"> <a 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Virus Research Panel </a> </li> <li class="menu-item"> <a href="/fr/products/ngs/fixed-panels/comprehensive-viral-research-panel" class=""> Comprehensive Viral Research Panel </a> </li> </ul> </div> </li> </ul> </div> <a href="https://www.twistbioscience.com/fr/products/precision-dx" class="promo-box"> <div class="promo-box__image"> <img src="https://www.twistbioscience.com/sites/default/files/2024-03/Twist%20Promo%20Banner%20-%20IVDR%20Clinical%20Icon-213x136-V1%20%281%29.jpg" alt="Flux de travail pour la préparation de banque d'exomes entiers de Twist. En savoir plus >"> </div> <p>Flux de travail pour la préparation de banque d'exomes entiers de Twist. En savoir plus ></p> </a> </div> <div class="primary-menu__dropdown-content" data-tab-drawer-4 data-index="data-tab-drawer-4"> <div class="primary-menu__dropdown-columns"> <h2> <a href="/fr/products/libraries"> <img src="https://www.twistbioscience.com/sites/default/files/2021-05/icon-libraries.png" alt="Banques de variants"> Banques de variants </a> </h2> <ul class="sub-nav-columns total-columns-2" data-sub-nav-columns="2"> <li data-sub-nav-column="1"></li> <li data-sub-nav-column="2"></li> </ul> <a href="/fr/get-quote" class="main-action">Commander maintenant</a> <ul class="menu menu-level-2"> <li class="menu-item" data-column-position=1 data-column-v-weight=1> <a href="/fr/tcr-libraries" class="menu-item level-2-category-item"> Nouvelles <span class="menu-link-new">banques TCR</span> </a> </li> <li class="menu-item" data-column-position=1 data-column-v-weight=1> <a href="/fr/products/libraries/combinatorial-variant-library" class="menu-item level-2-category-item"> CVL </a> </li> <li class="menu-item" data-column-position=2 data-column-v-weight=1> <a href="/fr/products/libraries/spread-out-low-diversity-libraries" class="menu-item level-2-category-item"> Banques SOLD </a> </li> <li class="menu-item" data-column-position=1 data-column-v-weight=1> <a href="/fr/products/libraries/site-saturation-libraries" class="menu-item level-2-category-item"> Banques de variants à saturation de site (SSVL) </a> </li> <li class="menu-item-as-title menu-item" data-column-position=2 data-column-v-weight=1> <a href="https://www.twistbioscience.com/fr/products/libraries/enzyme-screening-kit" class="menu-item-as-title menu-item level-2-category-item"> Kits de criblage enzymatique<span class="menu-link-new">Nouveau</span> </a> </li> </ul> </div> <a href="/fr/products/libraries?wvideo=da25dcguv3" class="promo-box"> <div class="promo-box__image"> <img src="https://www.twistbioscience.com/sites/default/files/2022-06/CVL-library-promo-426x272.png" alt="Découvrez comment Twist crée des banques de variants pour votre application parfaite."> </div> <p>Découvrez comment Twist crée des banques de variants pour votre application parfaite.</p> </a> </div> <div class="primary-menu__dropdown-content" data-tab-drawer-5 data-index="data-tab-drawer-5"> <div class="primary-menu__dropdown-columns"> <h2> <a href="/fr/products/ngs/synthetic-viral-controls"> <img src="https://www.twistbioscience.com/sites/default/files/2022-01/Synthetic%20Controls-1_0.png" alt="Contrôles synthétiques"> Contrôles synthétiques </a> </h2> <ul class="sub-nav-columns total-columns-2" data-sub-nav-columns="2"> <li data-sub-nav-column="1"></li> <li data-sub-nav-column="2"></li> </ul> <a href="https://ecommerce.twistdna.com/app/sars-cov-2" class="main-action">Commander maintenant</a> <ul class="menu menu-level-2"> <li class="menu-item menu-item--expanded level-2-category-section" data-column-position=1 data-column-v-weight=1> <div class="level-2-category-section "> <span class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> Biopsie liquide </span> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="/fr/products/ngs/twist-cfdna-pan-cancer-reference-standards" class=""> Modèles de référence d’ADN acellulaire (cfDNA) Pan-cancer v2<span class="menu-link-new">Nouveau</span> </a> </li> </ul> </div> </li> <li class="menu-item menu-item--expanded level-2-category-section" data-column-position=1 data-column-v-weight=1> <div class="level-2-category-section "> <a href="/fr/products/ngs/synthetic-viral-controls" class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> Maladie infectieuse </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="/fr/products/ngs/synthetic-viral-controls?tab=respiratory-virus-controls" class=""> Contrôles des virus respiratoires </a> </li> <li class="menu-item"> <a href="/fr/products/ngs/synthetic-viral-controls?tab=sars-cov-2-controls" class=""> Contrôles pour SARS-CoV-2 </a> </li> <li class="menu-item"> <a href="/fr/products/ngs/synthetic-viral-controls?tab=monkeypox-controls" class=""> Contrôles synthétiques du virus de la mpox </a> </li> </ul> </div> </li> </ul> </div> <a href="/fr/products/ngs/twist-cfdna-pan-cancer-reference-standards" class="promo-box"> <div class="promo-box__image"> <img src="https://www.twistbioscience.com/sites/default/files/2022-01/synthetic%20controls%20dev_0.png" alt="Découvrez nos nouveaux contrôles synthétiques pour le développement d’analyses de biopsie liquide."> </div> <p>Découvrez nos nouveaux contrôles synthétiques pour le développement d’analyses de biopsie liquide.</p> </a> </div> <div class="primary-menu__dropdown-content" data-tab-drawer-6 data-index="data-tab-drawer-6"> <div class="primary-menu__dropdown-columns"> <h2> <a href="/fr/twist-biopharma-solutions"> <img src="https://www.twistbioscience.com/sites/default/files/2021-05/icon-antibodies.png" alt="Recherche d’anticorps"> Recherche d’anticorps </a> </h2> <ul class="sub-nav-columns total-columns-2" data-sub-nav-columns="2"> <li data-sub-nav-column="1"></li> <li data-sub-nav-column="2"></li> </ul> <a href="https://ecommerce.twistdna.com/app/igg" class="main-action">Commander maintenant</a> <ul class="menu menu-level-2"> <li class="menu-item-as-title menu-item menu-item--expanded level-2-category-section" data-column-position=1 data-column-v-weight=1> <div class="level-2-category-section "> <a href="/fr/antibody-discovery/critical-reagents" class="menu-item-as-title menu-item menu-item--expanded level-2-category-section level-2-category-item"> Réactifs essentiels et anticorps outils </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="https://www.twistbioscience.com/fr/product/antibody-discovery/reagents-and-tools/anti-id" class=""> Anti-ID Antibody Discovery </a> </li> <li class="menu-item"> <a href="/fr/products/antibody-discovery/in-vivo/abveris-antigen-production-design" class=""> Génération d’antigènes </a> </li> </ul> </div> </li> <li class="menu-item menu-item--expanded level-2-category-section" data-column-position=1 data-column-v-weight=1> <div class="level-2-category-section "> <a href="/fr/antibody-discovery/in-vitro-antibody-discovery" class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> in vitro > </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="/fr/products/antibody-discovery/custom-library-design-and-generation" class=""> Génération de banques sur mesure </a> </li> <li class="menu-item"> <a href="/fr/products/antibody-discovery/library-of-libraries" class=""> La banque des banques </a> </li> <li class="menu-item"> <a href="/fr/products/antibody-discovery/phage-display-antibody-discovery" class=""> Expression phagique </a> </li> </ul> </div> </li> <li class="menu-item-as-title menu-item menu-item--expanded level-2-category-section" data-column-position=2 data-column-v-weight=1> <div class="level-2-category-section "> <a href="/fr/antibody-discovery/twist-antibody-optimization" class="menu-item-as-title menu-item menu-item--expanded level-2-category-section level-2-category-item"> Optimisation et ingénierie des anticorps </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="/fr/product/antibody-discovery/engineering-and-optimization/TAO" class=""> Twist Antibody Optimization Platform (TAO) </a> </li> <li class="menu-item"> <a href="/fr/product/antibody-discovery/engineering-and-optimization/affinity-maturation" class=""> Maturation des affinités par l’IA/AA </a> </li> <li class="menu-item"> <a href="/fr/product/antibody-discovery/engineering-and-optimization/humanization" class=""> Humanisation in silico </a> </li> </ul> </div> </li> <li class="menu-item menu-item--expanded level-2-category-section" data-column-position=2 data-column-v-weight=1> <div class="level-2-category-section "> <a href="/fr/antibody-discovery/in-vivo-antibody-discovery" class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> in vivo > </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="https://www.twistbioscience.com/fr/products/antibody-discovery/in-vivo/abveris-diversimab-hyperimmune-mouse-technology" class=""> Technologie Diversimab </a> </li> <li class="menu-item"> <a href="/fr/products/antibody-discovery/in-vivo/single-b-cell-screening" class=""> Criblage de lymphocytes B uniques </a> </li> <li class="menu-item"> <a href="/fr/products/antibody-discovery/in-vivo/abveris-antibody-discovery-services" class=""> Hybridoma </a> </li> </ul> </div> </li> <li class="menu-item-as-title menu-item menu-item--expanded level-2-category-section" data-column-position=2 data-column-v-weight=1> <div class="level-2-category-section "> <a href="/fr/antibody-discovery/high-throughput-antibody-production" class="menu-item-as-title menu-item menu-item--expanded level-2-category-section level-2-category-item"> Production d’anticorps personnalisés > </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="/fr/products/antibody-discovery/high-throughput-antibody-production?tab=cho-express-antibodies" class=""> CHO Express Antibodies </a> </li> <li class="menu-item"> <a href="/fr/products/antibody-discovery/high-throughput-antibody-production?tab=hek239-express-antibodies" class=""> Anticorps express HEK293 </a> </li> </ul> </div> </li> <li class="menu-item-as-title menu-item menu-item--expanded level-2-category-section" data-column-position=1 data-column-v-weight=1> <div class="level-2-category-section "> <a href="https://www.twistbioscience.com/fr/product/antibody-discovery/characterization" class="menu-item-as-title menu-item menu-item--expanded level-2-category-section level-2-category-item"> Caractérisation des anticorps > </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="https://www.twistbioscience.com/fr/product/antibody-discovery/characterization/SPR" class=""> Caractérisation biophysique par SPR </a> </li> <li class="menu-item"> <a href="/fr/product/antibody-discovery/characterization/developability" class=""> Évaluation de la développabilité </a> </li> <li class="menu-item"> <a href="/fr/product/antibody-discovery/characterization/functional-assays" class=""> Essais fonctionnels </a> </li> </ul> </div> </li> <li class="menu-item menu-item--expanded level-2-category-section" data-column-position=2 data-column-v-weight=1> <div class="level-2-category-section "> <a href="/fr/antibody-discovery/asset-licensing" class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> Octroi de licences pour les actifs > </a> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="/fr/products/biopharma/antibody-partnerships-and-collaborations" class=""> Partenariat et collaborations </a> </li> </ul> </div> </li> </ul> </div> <a href="/fr/resources/product-sheet/target-molecule" class="promo-box"> <div class="promo-box__image"> <img src="https://www.twistbioscience.com/sites/default/files/2021-05/biopharma-global-213x136%402x_0.png" alt="Lisez notre nouvel Ebook sur la recherche et le développement d'anticorps."> </div> <p>Lisez notre nouvel Ebook sur la recherche et le développement d'anticorps.</p> </a> </div> </div> </div> </div> </li> <li class="menu-item menu-item--expanded js-primary-menu-wide-mode"> <span title="" id="" name="" rel="" style="" target="" accesskey="">Applications</span> <div data-primary-nav-subnav-container class="wide-menu-sub-navigation"> <div class="primary-nav__drawer-side-bar"> <div class="menu-dropdown menu-dropdown-0"> <ul class="menu menu-level-1" data-tabs > <li class="menu-item menu-item--expanded" data-tab="data-tab-drawer-1" > <a href="https://www.twistbioscience.com/fr/good-fight-june-6"> <span>Recherche sur le cancer</span> </a> </li> <li class="menu-item menu-item--large-icon" > <a href="/fr/products/storage"> <span>Stockage de données sur l’ADN</span> </a> </li> <li 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menu-item--expanded level-2-category-section" data-column-position=1 data-column-v-weight=1> <div class="level-2-category-section "> <span class="menu-item menu-item--expanded level-2-category-section level-2-category-item"> Biopsie liquide </span> <ul class="menu menu-level-3"> <li class="menu-item"> <a href="https://www.twistbioscience.com/fr/products/ngs/library-preparation/cfDNA-library-prep-kit" class=""> <span class="menu-link-new">Nouveau</span>kit de préparation de banque cfDNA </a> </li> <li class="menu-item"> <a href="https://www.twistbioscience.com/fr/products/ngs/Twist-cfDNA-Pan-Cancer-Reference-Standard-v2" class=""> Modèles de référence d’ADN acellulaire (cfDNA) Pan-cancer v2<span class="menu-link-new">Nouveau</span> </a> </li> <li class="menu-item"> <a href="/fr/pages/personalized-sequencing" class=""> Maladie résiduelle minime (Minimal Residual Disease, MRD)<span class="menu-link-new">Nouveau</span> </a> </li> </ul> </div> </li> </ul> </div> <a href="/fr/pages/personalized-sequencing" class="promo-box"> <div class="promo-box__image"> <img src="https://www.twistbioscience.com/sites/default/files/2022-06/personalized-sequencing-promo-thumbnail-426x272_0.png" alt="En savoir plus sur le panel MRD Rapid 500 de Twist"> </div> <p>En savoir plus sur le panel MRD Rapid 500 de Twist</p> </a> </div> <div class="primary-menu__dropdown-content" data-tab-drawer-3 data-index="data-tab-drawer-3"> <div class="primary-menu__dropdown-columns"> <h2> <a href="https://www.twistbioscience.com/fr/drug-discovery"> Outils pour la découverte de médicaments </a> </h2> <ul class="sub-nav-columns total-columns-3" data-sub-nav-columns="3"> <li data-sub-nav-column="1"></li> <li data-sub-nav-column="2"></li> <li data-sub-nav-column="3"></li> </ul> <ul class="menu menu-level-2"> <li class="menu-item" data-column-position=1 data-column-v-weight=1> <a href="/fr/products/antibody-discovery/single-domain-vhh-antibody-discovery" class="menu-item 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about="/products/oligopools" class="node node--type-product-child node--view-mode-full product-style-teal product-oligo-pools" data-product="oligo-pools"> <div class="node__content"> <div class="field field--name-field-page-banner field--type-entity-reference-revisions field--label-hidden field__item"> <style> @media (min-width: 768px) { .paragraph--type--banner { background-image: url(https://www.twistbioscience.com/sites/default/files/2019-12/bg-oligo-pools%402x%20%281%29.png); background-size: cover; } } @media (max-width: 767px) { .paragraph--type--banner { background-image: url(https://www.twistbioscience.com/sites/default/files/2019-12/bg-oligo-pools%402x%20%281%29.png); background-size: cover; } } </style> <div class="banner paragraph paragraph--type--banner paragraph--view-mode--default"> <div class="banner__content"> <div class="banner-preview-image-wrapper tablet-preview-image"> </div> <div class="container"> <div class="row"> <div class="banner__title col-md-7"> <div class="title"> <h1>Pools d’oligonucléotides</h1> </div> </div> <div class="header-right-container-wrapper col-md-5"> </div> </div> </div> </div> </div> </div> <div class="system-picker-for-tabs d-md-none" > <div class="title-picker"> <span class="chosen-tab-title"></span> <select> <option value="1">PRÉSENTATION</option> <option value="2">POOLS D’OLIGONUCLÉOTIDES</option> <option value="3">CLONED OLIGO POOLS</option> <option value="4">APPLICATIONS</option> <option value="5">RESSOURCES</option> </select> </div> </div> <div class="system-sticky-tabs"> <div class="sticky-wrapper"> <div class="sticky-wrapper-tabs"> <span class="tab-item" data-id="1" data-twist-element="tab" data-twist-location="product-tabs-sticky" data-twist-type="tab">PRÉSENTATION</span> <span class="tab-item" data-id="2" data-twist-element="tab" data-twist-location="product-tabs-sticky" data-twist-type="tab">POOLS D’OLIGONUCLÉOTIDES</span> <span class="tab-item" data-id="3" data-twist-element="tab" data-twist-location="product-tabs-sticky" data-twist-type="tab">CLONED OLIGO POOLS</span> <span class="tab-item" data-id="4" data-twist-element="tab" data-twist-location="product-tabs-sticky" data-twist-type="tab">APPLICATIONS</span> <span class="tab-item" data-id="5" data-twist-element="tab" data-twist-location="product-tabs-sticky" data-twist-type="tab">RESSOURCES</span> </div> </div> </div> <div class="field field--name-field-pc-tabs field--type-entity-reference-revisions field--label-hidden field__items"> <div class="field__item select-picker-0 active-picker" data-id="0"> <div class="no-custom-show-more paragraph paragraph--type--section-tabs paragraph--view-mode--default array" data-style="array"> <div class="field field--name-field-cp-title field--type-string field--label-hidden field__item" id="overview">Présentation</div> <div class="field field--name-field-cp-paragraphs field--type-entity-reference-revisions field--label-hidden field__items"> <div class="desktop-layer"> <div class="container"> <div class="virtual-wrapper row"> <div class="virtual-region-left col-md-7"> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Performance quelle que soit l’échelle</h3> <p>Notre plateforme de synthèse d’ADN révolutionnaire, basée sur le silicone, est capable de produire plus d’un million d’oligos d’ADNss uniques en une seule fois. Chaque puce contient des milliers de clusters distincts, chacun contenant 121 surfaces adressables individuellement capables de synthétiser une séquence d’oligonucléotide unique. Une représentation de la technologie des puces en silicone se trouve ci-dessous.</p> <p>En raison de l’ampleur de la capacité de synthèse d’ADN sur la puce, il n’y a pratiquement aucune limite au nombre d’oligonucléotides uniques qui peuvent être assemblés dans un pool. Ne limitez pas le résultat de votre application, commandez des pools d’oligonucléotides spécifiquement adaptés à votre expérience. </p> <p><img alt="Performance quelle que soit l’échelle" class="twist-lightbox" data-entity-type="file" data-entity-uuid="a77acd33-1ffd-4d25-b533-429fda0fb0aa" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Performance-at-Any-Scale_0.png" title width="1400" height="728" data-mfp-src="/sites/default/files/inline-images/Performance-at-Any-Scale_0.png" loading="lazy"></p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h4>Plus d’espace pour l’innovation</h4> <p><span style="font-size:10.5pt; font-variant:normal; white-space:pre-wrap"><span style="font-family:'Proxima Nova',sans-serif"><span style="color:#313538"><span style="font-weight:400"><span style="font-style:normal"><span style="text-decoration:none">Ne soyez pas limité par la longueur. Encodez les éléments et les séquences dont vous avez besoin, y compris les promoteurs, les exhausteurs, les guides et les sondes, le tout dans une architecture étendue. Avec des longueurs d’oligo allant jusqu’à 300 nt, il est possible de coder plusieurs éléments dans une seule structure, ce qui vous permet d’en faire plus avec chaque oligonucléotide.</span></span></span></span></span></span></p> <h4><img alt="Plus d’espace pour l’innovation" class="twist-lightbox" data-entity-type="file" data-entity-uuid="29905968-d8e7-46f1-850f-4f6aff0f6d07" id src="https://www.twistbioscience.com/sites/default/files/inline-images/More-Space-Innovation_0.png" title width="1400" height="488" data-mfp-src="/sites/default/files/inline-images/More-Space-Innovation_0.png" loading="lazy"></h4> <h4>Criblage réussi en une seule fois</h4> <p><span style="font-size:10.5pt; font-variant:normal; white-space:pre-wrap"><span style="font-family:'Proxima Nova',sans-serif"><span style="color:#313538"><span style="font-weight:400"><span style="font-style:normal"><span style="text-decoration:none">L’uniformité inégalée du pool sans biais permet d’obtenir plus de cibles uniques par crible. Cela signifie que moins d’efforts sont nécessaires pour obtenir la couverture de données dont vous avez besoin, ce qui vous fait gagner du temps et de l’argent.</span></span></span></span></span></span></p> <p><img alt="Criblage réussi en une seule fois" class="twist-lightbox" data-entity-type="file" data-entity-uuid="e380530b-442c-4e6c-93b5-3957aba94d6b" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Frequency-Density-Log10-Read-Count_0.png" title width="1400" height="814" data-mfp-src="/sites/default/files/inline-images/Frequency-Density-Log10-Read-Count_0.png" loading="lazy"></p> <h4>Soyez sûr(e) de vos données</h4> <p>Grâce à la précision et à l’uniformité des pools d’oligonucléotides Twist Bioscience, vous pouvez être sûr(e) de la qualité de vos résultats expérimentaux. Ne perdez pas un seul élément de données en raison d’erreurs de synthèse ou du manque d’efficacité. Assurez la meilleure représentation des oligonucléotides et un faible taux d’erreur pour les oligonucléotides de toute longueur jusqu’à 300 nt, et les pools de toute taille. Optimisez ces pools d’oligo de qualité exceptionnelle à l’échelle et à la longueur précises requises pour votre application.</p> <p><img alt="Des données sûres" class="twist-lightbox" data-entity-type="file" data-entity-uuid="0d4dc639-3ae0-4622-bcdb-43b5f59e3b08" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Data-Confident_0.png" title width="1400" height="982" data-mfp-src="/sites/default/files/inline-images/Data-Confident_0.png" loading="lazy"></p> </div> </div> </div> <div class="virtual-region-right col-md-4"> <div class="virtual-region-sticky-col"> <div class="grey clearfix component-paragraph paragraph paragraph--type--component-callout paragraph--view-mode--default"> <div class="field field--name-field-cp-callout-title field--type-string field--label-hidden field__item">DES QUESTIONS ?</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Dites-nous ce sur quoi vous souhaitez obtenir plus d’informations. Nous sommes là pour vous aider !<br> <br> <a href="https://www.twistbioscience.com/fr/contact">Nous contacter</a></p></div> </div> <div class="grey clearfix component-paragraph paragraph paragraph--type--component-callout paragraph--view-mode--default"> <div class="field field--name-field-cp-callout-title field--type-string field--label-hidden field__item">POURQUOI L’UNIFORMITÉ EST-ELLE IMPORTANTE ?</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p><a href="https://www.twistbioscience.com/fr/blog/science/Uniformity-DNA-Synthesis">EN SAVOIR PLUS</a></p></div> </div> </div> </div> <div class="clearfix"></div> </div> </div> </div> <div class="mobile-layer"> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Performance quelle que soit l’échelle</h3> <p>Notre plateforme de synthèse d’ADN révolutionnaire, basée sur le silicone, est capable de produire plus d’un million d’oligos d’ADNss uniques en une seule fois. Chaque puce contient des milliers de clusters distincts, chacun contenant 121 surfaces adressables individuellement capables de synthétiser une séquence d’oligonucléotide unique. Une représentation de la technologie des puces en silicone se trouve ci-dessous.</p> <p>En raison de l’ampleur de la capacité de synthèse d’ADN sur la puce, il n’y a pratiquement aucune limite au nombre d’oligonucléotides uniques qui peuvent être assemblés dans un pool. Ne limitez pas le résultat de votre application, commandez des pools d’oligonucléotides spécifiquement adaptés à votre expérience. </p> <p><img alt="Performance quelle que soit l’échelle" class="twist-lightbox" data-entity-type="file" data-entity-uuid="a77acd33-1ffd-4d25-b533-429fda0fb0aa" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Performance-at-Any-Scale_0.png" title width="1400" height="728" data-mfp-src="/sites/default/files/inline-images/Performance-at-Any-Scale_0.png" loading="lazy"></p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h4>Plus d’espace pour l’innovation</h4> <p><span style="font-size:10.5pt; font-variant:normal; white-space:pre-wrap"><span style="font-family:'Proxima Nova',sans-serif"><span style="color:#313538"><span style="font-weight:400"><span style="font-style:normal"><span style="text-decoration:none">Ne soyez pas limité par la longueur. Encodez les éléments et les séquences dont vous avez besoin, y compris les promoteurs, les exhausteurs, les guides et les sondes, le tout dans une architecture étendue. Avec des longueurs d’oligo allant jusqu’à 300 nt, il est possible de coder plusieurs éléments dans une seule structure, ce qui vous permet d’en faire plus avec chaque oligonucléotide.</span></span></span></span></span></span></p> <h4><img alt="Plus d’espace pour l’innovation" class="twist-lightbox" data-entity-type="file" data-entity-uuid="29905968-d8e7-46f1-850f-4f6aff0f6d07" id src="https://www.twistbioscience.com/sites/default/files/inline-images/More-Space-Innovation_0.png" title width="1400" height="488" data-mfp-src="/sites/default/files/inline-images/More-Space-Innovation_0.png" loading="lazy"></h4> <h4>Criblage réussi en une seule fois</h4> <p><span style="font-size:10.5pt; font-variant:normal; white-space:pre-wrap"><span style="font-family:'Proxima Nova',sans-serif"><span style="color:#313538"><span style="font-weight:400"><span style="font-style:normal"><span style="text-decoration:none">L’uniformité inégalée du pool sans biais permet d’obtenir plus de cibles uniques par crible. Cela signifie que moins d’efforts sont nécessaires pour obtenir la couverture de données dont vous avez besoin, ce qui vous fait gagner du temps et de l’argent.</span></span></span></span></span></span></p> <p><img alt="Criblage réussi en une seule fois" class="twist-lightbox" data-entity-type="file" data-entity-uuid="e380530b-442c-4e6c-93b5-3957aba94d6b" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Frequency-Density-Log10-Read-Count_0.png" title width="1400" height="814" data-mfp-src="/sites/default/files/inline-images/Frequency-Density-Log10-Read-Count_0.png" loading="lazy"></p> <h4>Soyez sûr(e) de vos données</h4> <p>Grâce à la précision et à l’uniformité des pools d’oligonucléotides Twist Bioscience, vous pouvez être sûr(e) de la qualité de vos résultats expérimentaux. Ne perdez pas un seul élément de données en raison d’erreurs de synthèse ou du manque d’efficacité. Assurez la meilleure représentation des oligonucléotides et un faible taux d’erreur pour les oligonucléotides de toute longueur jusqu’à 300 nt, et les pools de toute taille. Optimisez ces pools d’oligo de qualité exceptionnelle à l’échelle et à la longueur précises requises pour votre application.</p> <p><img alt="Des données sûres" class="twist-lightbox" data-entity-type="file" data-entity-uuid="0d4dc639-3ae0-4622-bcdb-43b5f59e3b08" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Data-Confident_0.png" title width="1400" height="982" data-mfp-src="/sites/default/files/inline-images/Data-Confident_0.png" loading="lazy"></p> </div> </div> <div class="grey clearfix component-paragraph paragraph paragraph--type--component-callout paragraph--view-mode--default"> <div class="field field--name-field-cp-callout-title field--type-string field--label-hidden field__item">DES QUESTIONS ?</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Dites-nous ce sur quoi vous souhaitez obtenir plus d’informations. Nous sommes là pour vous aider !<br> <br> <a href="https://www.twistbioscience.com/fr/contact">Nous contacter</a></p></div> </div> <div class="grey clearfix component-paragraph paragraph paragraph--type--component-callout paragraph--view-mode--default"> <div class="field field--name-field-cp-callout-title field--type-string field--label-hidden field__item">POURQUOI L’UNIFORMITÉ EST-ELLE IMPORTANTE ?</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p><a href="https://www.twistbioscience.com/fr/blog/science/Uniformity-DNA-Synthesis">EN SAVOIR PLUS</a></p></div> </div> </div> </div> </div> </div> <div class="field__item select-picker-1" data-id="1"> <div class="no-custom-show-more paragraph paragraph--type--section-tabs paragraph--view-mode--default array" data-style="array"> <div class="field field--name-field-cp-title field--type-string field--label-hidden field__item" id="oligo-pools">Pools d’oligonucléotides</div> <div class="field field--name-field-cp-paragraphs field--type-entity-reference-revisions field--label-hidden field__items"> <div class="desktop-layer"> <div class="container"> <div class="virtual-wrapper row"> <div class="virtual-region-left col-md-7"> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Pools d’oligonucléotides</h3> <p>Les Twist Oligo Pools sont des ensembles très divers d’oligonucléotides à simple brin synthétisés à l’aide de notre technologie d’écriture de l’ADN sur silicone. Notre plateforme de synthèse permet la production massivement parallèle de centaines de milliers d’oligonucléotides précis et de haute qualité par cycle, ce qui permet de générer des pools d’oligonucléotides complexes et diversifiés pour des applications telles que le criblage CRISPR.</p> <h4>Comment cela fonctionne-t-il ?</h4> <p>Vous nous fournissez vos séquences d’oligonucléotides et nous synthétisons les banques de pools d’oligonucléotides conçus par l’utilisateur, ce qui vous permet de consacrer votre temps à l’expérimentation et à la découverte. </p> <p><img alt="Banques" class="twist-lightbox" data-entity-type="file" data-entity-uuid="12ddd906-5011-418a-af8f-47244e1e1a78" height="176" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Libraries%20Made%20Easy%20from%20Design%20to%20Build.png" title width="572" data-mfp-src="/sites/default/files/inline-images/Libraries%20Made%20Easy%20from%20Design%20to%20Build.png" loading="lazy"></p> <h4>Synthèse d’oligonucléotides extrêmement uniforme</h4> <p>Les données de contrôle de qualité NGS provenant d’un pool d’oligonucléotides standard contenant 23 000 oligonucléotides 90-mer montrent l’uniformité du pool à une couverture de lecture de 300x. Le tableau correspondant indique les paramètres d’uniformité pour ce pool. Les oligonucléotides Twist Bioscience sont synthétisés sans biais avec une grande uniformité et une représentation complète des oligonucléotides.</p> <p><img alt="Comptage de lectures d’oligonucléotides" class="twist-lightbox" data-entity-type="file" data-entity-uuid="2ad960eb-4262-4e5f-b5cd-2cbd4a4fbb38" height="389" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Oligo-Pools-Read-Counts-Histogram-Uniformity.png" title width="490" data-mfp-src="/sites/default/files/inline-images/Oligo-Pools-Read-Counts-Histogram-Uniformity.png" loading="lazy"></p> <h4>Analyse de séquençage des pool d’oligonucléotides</h4> <p>L’analyse de séquençage des pools d’oligonucléotides générés par Twist Bioscience et d’un concurrent de type matrice démontre que les pools d’oligonucléotide Twist Bioscience contiennent 100 % des séquences escomptées et un pourcentage plus élevé de séquences correctes que le pool du concurrent.</p> <p><img alt="Analyse" class="twist-lightbox" data-entity-type="file" data-entity-uuid="d205b877-099c-48fa-89a9-50a2353f8dbb" height="194" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Oligo-Pools-Sequencing-Analysis.png" title width="706" data-mfp-src="/sites/default/files/inline-images/Oligo-Pools-Sequencing-Analysis.png" loading="lazy"></p> <hr> <h4>Spécifications</h4> <table border="0" cellpadding="1" cellspacing="1" style="width: 500px;"> <tbody> <tr> <td style="width: 243px;">Longueur oligonucléotide</td> <td style="width: 251px;">Jusqu’à 300 nt</td> </tr> <tr> <td style="width: 243px;">Taille du pool d'oligonucléotides</td> <td style="width: 251px;">Aucune limite sur la taille du pool d’oligonucléotides</td> </tr> <tr> <td style="width: 243px;">Rendement</td> <td style="width: 251px;">>0,2 fmol moyen de chaque oligonucléotide</td> </tr> <tr> <td style="width: 243px;">Uniformité</td> <td style="width: 251px;">>90 % oligonucléotides représentés dans un écart < 2,0x de la moyenne</td> </tr> <tr> <td style="width: 243px;">Taux d’erreur</td> <td style="width: 251px;">Jusqu’à 1 : 3000</td> </tr> <tr> <td style="width: 243px;">Délais d’exécution</td> <td style="width: 251px;">En seulement 3 jours*</td> </tr> <tr> <td style="width: 243px;">Prix</td> <td style="width: 251px;">Prix parmi les meilleurs du secteur</td> </tr> </tbody> </table> <p> </p> <p class="size12 gray-light">*Le délai d'exécution pour l'offre standard de pools d'oligonucléotides varie en fonction de la longueur et de la complexité de la séquence. Les pools d'oligonucléotides d'une longueur de 20 à 121 nucléotides varient entre 3 à 6 jours ouvrables, ceux de 121 à 200 nucléotides varient entre 3 à 8 jours ouvrables, et ceux de 201 à 300 nucléotides varient entre 5 à 10 jours ouvrables.</p> <p> </p> <p> </p> </div> </div> </div> <div class="virtual-region-right col-md-4"> <div class="virtual-region-sticky-col"> <div class="grey clearfix component-paragraph paragraph paragraph--type--component-callout paragraph--view-mode--default"> <div class="field field--name-field-cp-callout-title field--type-string field--label-hidden field__item">DES QUESTIONS ?</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Dites-nous ce sur quoi vous souhaitez obtenir plus d’informations. Nous sommes là pour vous aider !<br> <br> <a href="https://www.twistbioscience.com/fr/contact">Nous contacter</a></p></div> </div> </div> </div> <div class="clearfix"></div> </div> </div> </div> <div class="mobile-layer"> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Pools d’oligonucléotides</h3> <p>Les Twist Oligo Pools sont des ensembles très divers d’oligonucléotides à simple brin synthétisés à l’aide de notre technologie d’écriture de l’ADN sur silicone. Notre plateforme de synthèse permet la production massivement parallèle de centaines de milliers d’oligonucléotides précis et de haute qualité par cycle, ce qui permet de générer des pools d’oligonucléotides complexes et diversifiés pour des applications telles que le criblage CRISPR.</p> <h4>Comment cela fonctionne-t-il ?</h4> <p>Vous nous fournissez vos séquences d’oligonucléotides et nous synthétisons les banques de pools d’oligonucléotides conçus par l’utilisateur, ce qui vous permet de consacrer votre temps à l’expérimentation et à la découverte. </p> <p><img alt="Banques" class="twist-lightbox" data-entity-type="file" data-entity-uuid="12ddd906-5011-418a-af8f-47244e1e1a78" height="176" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Libraries%20Made%20Easy%20from%20Design%20to%20Build.png" title width="572" data-mfp-src="/sites/default/files/inline-images/Libraries%20Made%20Easy%20from%20Design%20to%20Build.png" loading="lazy"></p> <h4>Synthèse d’oligonucléotides extrêmement uniforme</h4> <p>Les données de contrôle de qualité NGS provenant d’un pool d’oligonucléotides standard contenant 23 000 oligonucléotides 90-mer montrent l’uniformité du pool à une couverture de lecture de 300x. Le tableau correspondant indique les paramètres d’uniformité pour ce pool. Les oligonucléotides Twist Bioscience sont synthétisés sans biais avec une grande uniformité et une représentation complète des oligonucléotides.</p> <p><img alt="Comptage de lectures d’oligonucléotides" class="twist-lightbox" data-entity-type="file" data-entity-uuid="2ad960eb-4262-4e5f-b5cd-2cbd4a4fbb38" height="389" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Oligo-Pools-Read-Counts-Histogram-Uniformity.png" title width="490" data-mfp-src="/sites/default/files/inline-images/Oligo-Pools-Read-Counts-Histogram-Uniformity.png" loading="lazy"></p> <h4>Analyse de séquençage des pool d’oligonucléotides</h4> <p>L’analyse de séquençage des pools d’oligonucléotides générés par Twist Bioscience et d’un concurrent de type matrice démontre que les pools d’oligonucléotide Twist Bioscience contiennent 100 % des séquences escomptées et un pourcentage plus élevé de séquences correctes que le pool du concurrent.</p> <p><img alt="Analyse" class="twist-lightbox" data-entity-type="file" data-entity-uuid="d205b877-099c-48fa-89a9-50a2353f8dbb" height="194" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Oligo-Pools-Sequencing-Analysis.png" title width="706" data-mfp-src="/sites/default/files/inline-images/Oligo-Pools-Sequencing-Analysis.png" loading="lazy"></p> <hr> <h4>Spécifications</h4> <table border="0" cellpadding="1" cellspacing="1" style="width: 500px;"> <tbody> <tr> <td style="width: 243px;">Longueur oligonucléotide</td> <td style="width: 251px;">Jusqu’à 300 nt</td> </tr> <tr> <td style="width: 243px;">Taille du pool d'oligonucléotides</td> <td style="width: 251px;">Aucune limite sur la taille du pool d’oligonucléotides</td> </tr> <tr> <td style="width: 243px;">Rendement</td> <td style="width: 251px;">>0,2 fmol moyen de chaque oligonucléotide</td> </tr> <tr> <td style="width: 243px;">Uniformité</td> <td style="width: 251px;">>90 % oligonucléotides représentés dans un écart < 2,0x de la moyenne</td> </tr> <tr> <td style="width: 243px;">Taux d’erreur</td> <td style="width: 251px;">Jusqu’à 1 : 3000</td> </tr> <tr> <td style="width: 243px;">Délais d’exécution</td> <td style="width: 251px;">En seulement 3 jours*</td> </tr> <tr> <td style="width: 243px;">Prix</td> <td style="width: 251px;">Prix parmi les meilleurs du secteur</td> </tr> </tbody> </table> <p> </p> <p class="size12 gray-light">*Le délai d'exécution pour l'offre standard de pools d'oligonucléotides varie en fonction de la longueur et de la complexité de la séquence. Les pools d'oligonucléotides d'une longueur de 20 à 121 nucléotides varient entre 3 à 6 jours ouvrables, ceux de 121 à 200 nucléotides varient entre 3 à 8 jours ouvrables, et ceux de 201 à 300 nucléotides varient entre 5 à 10 jours ouvrables.</p> <p> </p> <p> </p> </div> </div> <div class="grey clearfix component-paragraph paragraph paragraph--type--component-callout paragraph--view-mode--default"> <div class="field field--name-field-cp-callout-title field--type-string field--label-hidden field__item">DES QUESTIONS ?</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Dites-nous ce sur quoi vous souhaitez obtenir plus d’informations. Nous sommes là pour vous aider !<br> <br> <a href="https://www.twistbioscience.com/fr/contact">Nous contacter</a></p></div> </div> </div> </div> </div> </div> <div class="field__item select-picker-2" data-id="2"> <div class="no-custom-show-more paragraph paragraph--type--section-tabs paragraph--view-mode--default array" data-style="array"> <div class="field field--name-field-cp-title field--type-string field--label-hidden field__item" id="cloned-oligo-pools">Cloned Oligo Pools</div> <div class="field field--name-field-cp-paragraphs field--type-entity-reference-revisions field--label-hidden field__items"> <div class="desktop-layer"> <div class="container"> <div class="virtual-wrapper row"> <div class="virtual-region-left col-md-7"> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Cloned Oligo Pools</h3> <p>La qualité des pools d’oligonucléotides est la base d’une expérience réussie. Les erreurs de synthèse ou de clonage peuvent facilement fausser la qualité des pools d’oligonucléotides, entraînant une sur- ou une sous-représentation des séquences souhaitées. Twist propose désormais un service de clonage optimisé qui vous permet d’éviter davantage les difficultés liées à l’essai des conditions d’amplification de la PCR, à la sélection des polymérases et des paires d’amorces appropriées, ainsi qu’à la conception d’un flux de travail de clonage. Évitez les problèmes et laissez Twist synthétiser et cloner des pools d’oligonucléotides pour vous.  </p> <h4>Comment cela fonctionne-t-il ?</h4> <p style="line-height:1.38; margin-bottom:11px">À deux pas de vos pools d'oligonucléotides clonés personnalisés. Vous nous fournissez vos séquences d’oligonucléotides et nous synthétisons, amplifions et clonons les banques de pools d’oligonucléotides conçus par l’utilisateur, ce qui vous permet de consacrer votre temps à l’expérimentation et à la découverte. </p> <p><img alt="flux" class="twist-lightbox" data-entity-type="file" data-entity-uuid="9b1e361f-c5b2-4752-bc15-65728c8c90ce" id src="https://www.twistbioscience.com/sites/default/files/inline-images/resized-email-notitle-oligo-pools-workflow%402x.png" title width="1200" height="172" data-mfp-src="/sites/default/files/inline-images/resized-email-notitle-oligo-pools-workflow%402x.png" loading="lazy"></p> <h4>Les pools d'oligonucléotides clonés de Twist Bioscience, quelle que soit leur longueur, présentent une grande uniformité et un faible taux d'erreur</h4> <h5>Données de performance des pools d'oligonucléotides amplifiés et clonés d'une longueur inférieure à 150 nucléotides, même avec un contenu GC élevé :</h5> <p><img alt="Uniformité" class="twist-lightbox" data-entity-type="file" data-entity-uuid="f10e572a-db09-4573-b7fb-f49252ee4271" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Uniformity%20of%20linear%20and%20cloned%20oligo%20pools_0.png" title width="1401" height="588" data-mfp-src="/sites/default/files/inline-images/Uniformity%20of%20linear%20and%20cloned%20oligo%20pools_0.png" loading="lazy"></p> <hr> <p style="line-height:1.38; margin-bottom:11px"><img alt="pools d’oligonucléotides" class="twist-lightbox" data-entity-type="file" data-entity-uuid="0cecb1ac-e02f-4f45-8968-62712e123d75" id src="https://www.twistbioscience.com/sites/default/files/inline-images/edited-High%20quality%20cloned%20oligo%20pools.png" title width="1400" height="707" data-mfp-src="/sites/default/files/inline-images/edited-High%20quality%20cloned%20oligo%20pools.png" loading="lazy"></p> <h5 style="line-height: 1.38; margin-bottom: 11px;">Données de performance des pools d'oligonucléotides amplifiés et clonés d'une longueur allant jusqu’à 300 nucléotides, même avec un contenu GC élevé :<br><br />  </h5> <p><img alt="uniformité" class="twist-lightbox" data-entity-type="file" data-entity-uuid="01365214-e987-4c07-a3f9-96493c288c5b" id src="https://www.twistbioscience.com/sites/default/files/inline-images/cloned%20300mer%20uniformity%20-%20detailed.png" title width="1400" height="774" data-mfp-src="/sites/default/files/inline-images/cloned%20300mer%20uniformity%20-%20detailed.png" loading="lazy"></p> <hr> <p><img alt="Uniformité 2" class="twist-lightbox" data-entity-type="file" data-entity-uuid="aff55c5b-a144-46b7-acff-172710c4d32f" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Uniformity%20of%20linear%20and%20cloned%20oligo%20pools-1.png" title width="1400" height="1689" data-mfp-src="/sites/default/files/inline-images/Uniformity%20of%20linear%20and%20cloned%20oligo%20pools-1.png" loading="lazy"></p> <h4 style="line-height: 1.38;">Obtenez une qualité et une précision optimales de vos données expérimentales</h4> <p style="line-height: 1.38;">Les pools d'oligonucléotides clonés de Twist présentent non seulement un faible taux d'erreur et une grande uniformité, mais aussi un faible taux de chimères. Les chimères sont des molécules hybrides indésirables résultant de conditions de PCR sous-optimales qui mènent à une amplification et à une recombinaison incorrectes de différents oligonucléotides au sein du pool d'oligonucléotides. Consultez notre illustration ci-dessous pour un exemple d'un pool cloné sur la cible par rapport à un pool cloné hors cible qui contient des chimères, ce qui mène finalement à des pools d'oligonucléotides clonés qui sont composés de séquences non désirées : </p> <p><img alt="chimère" class="twist-lightbox" data-entity-type="file" data-entity-uuid="b3491b2f-2427-46ae-8715-1ef5ac206905" id src="https://www.twistbioscience.com/sites/default/files/inline-images/chimera.png" title width="1400" height="689" data-mfp-src="/sites/default/files/inline-images/chimera.png" loading="lazy"><br><br />  </p> <p><strong>Avec Twist, vous n'avez plus à vous soucier des chimères ! Vérifiez par vous-même :</strong></p> <p><br><br /> Amplification traditionnelle vs performance de la méthode d'amplification Twist en interne</p> <p style="line-height:1.38"><img alt="pools d’oligonucléotides" class="twist-lightbox" data-entity-type="file" data-entity-uuid="fc19a189-d8bb-4266-91dd-4597191aa3e4" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Oligo-Pools-Sequencing-Analysis_0.png" title width="1400" height="577" data-mfp-src="/sites/default/files/inline-images/Oligo-Pools-Sequencing-Analysis_0.png" loading="lazy"></p> <h4>Spécifications</h4> <table border="0" cellpadding="1" cellspacing="1" style="width: 500px;"> <tbody> <tr> <td style="width: 243px;">Longueur oligonucléotide</td> <td style="width: 251px;">Jusqu’à 300 nt</td> </tr> <tr> <td style="width: 243px;">Taille du pool d'oligonucléotides</td> <td style="width: 251px;">Aucune limite sur la taille du pool d’oligonucléotides</td> </tr> <tr> <td style="width: 243px;">Rendement</td> <td style="width: 251px;">>0,2 fmol moyen de chaque oligonucléotide</td> </tr> <tr> <td style="width: 243px;">Uniformité</td> <td style="width: 251px;">>90% oligos represented within <5.0x of the mean</td> </tr> <tr> <td style="width: 243px;">Chimera Rate</td> <td style="width: 251px;">As low as 1,5%*</td> </tr> <tr> <td style="width: 243px;">Délais d’exécution</td> <td style="width: 251px;">As few as 4 weeks*</td> </tr> </tbody> </table> <p> </p> <p class="size12 gray-light">*Conditions générales : Le délai d'exécution de base pour les pool d'oligonucléotides clonés, qui comprend le clonage et l'amplification, jusqu'à 100 et 300 nucléotides est de 4 à 6 semaines et 6 à 8 semaines, respectivement. Un délai supplémentaire de 2 semaines sera ajouté à ce délai de base pour tous les pool d'oligonucléotides clonés, quelle que soit leur longueur, qui nécessitent l'intégration d'un nouveau vecteur. Le taux de chimères, le taux de pertes et l'uniformité varient en fonction de la complexité de la séquence.</p> <p> </p> </div> </div> </div> <div class="virtual-region-right col-md-4"> <div class="virtual-region-sticky-col"> <div class="grey clearfix component-paragraph paragraph paragraph--type--component-callout paragraph--view-mode--default"> <div class="field field--name-field-cp-callout-title field--type-string field--label-hidden field__item">DES QUESTIONS ?</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Dites-nous ce sur quoi vous souhaitez obtenir plus d’informations. Nous sommes là pour vous aider !<br> <br> <a href="https://www.twistbioscience.com/fr/contact">Nous contacter</a></p></div> </div> </div> </div> <div class="clearfix"></div> </div> </div> </div> <div class="mobile-layer"> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Cloned Oligo Pools</h3> <p>La qualité des pools d’oligonucléotides est la base d’une expérience réussie. Les erreurs de synthèse ou de clonage peuvent facilement fausser la qualité des pools d’oligonucléotides, entraînant une sur- ou une sous-représentation des séquences souhaitées. Twist propose désormais un service de clonage optimisé qui vous permet d’éviter davantage les difficultés liées à l’essai des conditions d’amplification de la PCR, à la sélection des polymérases et des paires d’amorces appropriées, ainsi qu’à la conception d’un flux de travail de clonage. Évitez les problèmes et laissez Twist synthétiser et cloner des pools d’oligonucléotides pour vous.  </p> <h4>Comment cela fonctionne-t-il ?</h4> <p style="line-height:1.38; margin-bottom:11px">À deux pas de vos pools d'oligonucléotides clonés personnalisés. Vous nous fournissez vos séquences d’oligonucléotides et nous synthétisons, amplifions et clonons les banques de pools d’oligonucléotides conçus par l’utilisateur, ce qui vous permet de consacrer votre temps à l’expérimentation et à la découverte. </p> <p><img alt="flux" class="twist-lightbox" data-entity-type="file" data-entity-uuid="9b1e361f-c5b2-4752-bc15-65728c8c90ce" id src="https://www.twistbioscience.com/sites/default/files/inline-images/resized-email-notitle-oligo-pools-workflow%402x.png" title width="1200" height="172" data-mfp-src="/sites/default/files/inline-images/resized-email-notitle-oligo-pools-workflow%402x.png" loading="lazy"></p> <h4>Les pools d'oligonucléotides clonés de Twist Bioscience, quelle que soit leur longueur, présentent une grande uniformité et un faible taux d'erreur</h4> <h5>Données de performance des pools d'oligonucléotides amplifiés et clonés d'une longueur inférieure à 150 nucléotides, même avec un contenu GC élevé :</h5> <p><img alt="Uniformité" class="twist-lightbox" data-entity-type="file" data-entity-uuid="f10e572a-db09-4573-b7fb-f49252ee4271" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Uniformity%20of%20linear%20and%20cloned%20oligo%20pools_0.png" title width="1401" height="588" data-mfp-src="/sites/default/files/inline-images/Uniformity%20of%20linear%20and%20cloned%20oligo%20pools_0.png" loading="lazy"></p> <hr> <p style="line-height:1.38; margin-bottom:11px"><img alt="pools d’oligonucléotides" class="twist-lightbox" data-entity-type="file" data-entity-uuid="0cecb1ac-e02f-4f45-8968-62712e123d75" id src="https://www.twistbioscience.com/sites/default/files/inline-images/edited-High%20quality%20cloned%20oligo%20pools.png" title width="1400" height="707" data-mfp-src="/sites/default/files/inline-images/edited-High%20quality%20cloned%20oligo%20pools.png" loading="lazy"></p> <h5 style="line-height: 1.38; margin-bottom: 11px;">Données de performance des pools d'oligonucléotides amplifiés et clonés d'une longueur allant jusqu’à 300 nucléotides, même avec un contenu GC élevé :<br><br />  </h5> <p><img alt="uniformité" class="twist-lightbox" data-entity-type="file" data-entity-uuid="01365214-e987-4c07-a3f9-96493c288c5b" id src="https://www.twistbioscience.com/sites/default/files/inline-images/cloned%20300mer%20uniformity%20-%20detailed.png" title width="1400" height="774" data-mfp-src="/sites/default/files/inline-images/cloned%20300mer%20uniformity%20-%20detailed.png" loading="lazy"></p> <hr> <p><img alt="Uniformité 2" class="twist-lightbox" data-entity-type="file" data-entity-uuid="aff55c5b-a144-46b7-acff-172710c4d32f" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Uniformity%20of%20linear%20and%20cloned%20oligo%20pools-1.png" title width="1400" height="1689" data-mfp-src="/sites/default/files/inline-images/Uniformity%20of%20linear%20and%20cloned%20oligo%20pools-1.png" loading="lazy"></p> <h4 style="line-height: 1.38;">Obtenez une qualité et une précision optimales de vos données expérimentales</h4> <p style="line-height: 1.38;">Les pools d'oligonucléotides clonés de Twist présentent non seulement un faible taux d'erreur et une grande uniformité, mais aussi un faible taux de chimères. Les chimères sont des molécules hybrides indésirables résultant de conditions de PCR sous-optimales qui mènent à une amplification et à une recombinaison incorrectes de différents oligonucléotides au sein du pool d'oligonucléotides. Consultez notre illustration ci-dessous pour un exemple d'un pool cloné sur la cible par rapport à un pool cloné hors cible qui contient des chimères, ce qui mène finalement à des pools d'oligonucléotides clonés qui sont composés de séquences non désirées : </p> <p><img alt="chimère" class="twist-lightbox" data-entity-type="file" data-entity-uuid="b3491b2f-2427-46ae-8715-1ef5ac206905" id src="https://www.twistbioscience.com/sites/default/files/inline-images/chimera.png" title width="1400" height="689" data-mfp-src="/sites/default/files/inline-images/chimera.png" loading="lazy"><br><br />  </p> <p><strong>Avec Twist, vous n'avez plus à vous soucier des chimères ! Vérifiez par vous-même :</strong></p> <p><br><br /> Amplification traditionnelle vs performance de la méthode d'amplification Twist en interne</p> <p style="line-height:1.38"><img alt="pools d’oligonucléotides" class="twist-lightbox" data-entity-type="file" data-entity-uuid="fc19a189-d8bb-4266-91dd-4597191aa3e4" id src="https://www.twistbioscience.com/sites/default/files/inline-images/Oligo-Pools-Sequencing-Analysis_0.png" title width="1400" height="577" data-mfp-src="/sites/default/files/inline-images/Oligo-Pools-Sequencing-Analysis_0.png" loading="lazy"></p> <h4>Spécifications</h4> <table border="0" cellpadding="1" cellspacing="1" style="width: 500px;"> <tbody> <tr> <td style="width: 243px;">Longueur oligonucléotide</td> <td style="width: 251px;">Jusqu’à 300 nt</td> </tr> <tr> <td style="width: 243px;">Taille du pool d'oligonucléotides</td> <td style="width: 251px;">Aucune limite sur la taille du pool d’oligonucléotides</td> </tr> <tr> <td style="width: 243px;">Rendement</td> <td style="width: 251px;">>0,2 fmol moyen de chaque oligonucléotide</td> </tr> <tr> <td style="width: 243px;">Uniformité</td> <td style="width: 251px;">>90% oligos represented within <5.0x of the mean</td> </tr> <tr> <td style="width: 243px;">Chimera Rate</td> <td style="width: 251px;">As low as 1,5%*</td> </tr> <tr> <td style="width: 243px;">Délais d’exécution</td> <td style="width: 251px;">As few as 4 weeks*</td> </tr> </tbody> </table> <p> </p> <p class="size12 gray-light">*Conditions générales : Le délai d'exécution de base pour les pool d'oligonucléotides clonés, qui comprend le clonage et l'amplification, jusqu'à 100 et 300 nucléotides est de 4 à 6 semaines et 6 à 8 semaines, respectivement. Un délai supplémentaire de 2 semaines sera ajouté à ce délai de base pour tous les pool d'oligonucléotides clonés, quelle que soit leur longueur, qui nécessitent l'intégration d'un nouveau vecteur. Le taux de chimères, le taux de pertes et l'uniformité varient en fonction de la complexité de la séquence.</p> <p> </p> </div> </div> <div class="grey clearfix component-paragraph paragraph paragraph--type--component-callout paragraph--view-mode--default"> <div class="field field--name-field-cp-callout-title field--type-string field--label-hidden field__item">DES QUESTIONS ?</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Dites-nous ce sur quoi vous souhaitez obtenir plus d’informations. Nous sommes là pour vous aider !<br> <br> <a href="https://www.twistbioscience.com/fr/contact">Nous contacter</a></p></div> </div> </div> </div> </div> </div> <div class="field__item select-picker-3" data-id="3"> <div class="no-custom-show-more paragraph paragraph--type--section-tabs paragraph--view-mode--default array" data-style="array"> <div class="field field--name-field-cp-title field--type-string field--label-hidden field__item" id="applications">Applications</div> <div class="field field--name-field-cp-paragraphs field--type-entity-reference-revisions field--label-hidden field__items"> <div class="desktop-layer"> <div class="container"> <div class="virtual-wrapper row"> <div class="virtual-region-left col-md-12"> <div class="clearfix component-paragraph with-divider paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Banques d’ARN guides pour les cribles CRISPR</h3> <p><span><span><span><span><span><span>Les cribles CRISPR comportent l’élimination, l’interférence ou la modulation précises et à haut débit de l’activité des gènes, ce qui permet de révéler des interactions génotype-phénotype complexes. Les pool d’oligonucléotides sont conçus pour coder plusieurs molécules d’ARN guide par gène.  </span></span></span></span></span></span></p> <p><span><span><span><strong><span><span>Les avantages du produit sont les suivants :</span></span></strong></span></span></span></p> <ul> <li><span><span><span><span><span><span>Un contrôle total sur la conception de la banque de guides, évitant les guides non désirés, le gaspillage de séquençage et les ratios limités de guides par gène.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>L’association d’enzymes Cas haute fidélité et d’une synthèse très précise pour des cribles haute fidélité avec des effets hors cible minimaux.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Des pools incroyablement uniformes et sans biais permettent d’obtenir un rapport signal/bruit amplifié pour une détection maximale des résultats, ce qui réduit les coûts en aval.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Les oligonucléotides allant jusqu’à 300 nt libèrent de l’espace pour des éléments expérimentaux supplémentaires. Il est facile d’ajouter des guides multiples par oligonucléotide, des homologies génomiques ou des promoteurs minimaux.</span></span></span></span></span></span></li> </ul> <p><a href="https://www.twistbioscience.com/fr/resources/webinar/scalable-and-combinatorial-single-cell-crispr-screens-direct-guide-rna-capture"><img alt="Next Generation CRISPR Screens" src="https://www.twistbioscience.com/sites/default/files/inline-images/Next-generation-CRISPR-screens-based-on-single-cell-transcriptomics-11.24_1.png"></a></p> <p><span><span><span><span><span><span><span><span>Assistez à notre webinaire : <a class="green-link" href="/fr/resources/webinar/scalable-and-combinatorial-single-cell-crispr-screens-direct-guide-rna-capture"> Criblages CRISPR unicellulaires évolutifs et combinatoires par capture d’ARN directe et séquençage ciblé</a></span></span></span></span></span></span></span></span></p> </div> </div> <div class="clearfix component-paragraph with-divider paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Banques de données génomiques relatives aux puces à sonde recouvrantes</h3> <p><span><span><span><span><span><span>Les banques de puces permettent une étude complète de la fonctionnalité génomique. Les pools d’oligonucléotides peuvent coder des jeux ordonnés de peptides, des séquences codant des ARNm ou des fragments de génome.</span></span></span></span></span></span></p> <p><span><span><span><strong><span><span>Les avantages du produit sont les suivants :</span></span></strong></span></span></span></p> <ul> <li><span><span><span><span><span><span>Le « tiling » détermine les régions génomiques d’intérêt. Réduire le bruit expérimental en excluant les séquences indésirables à l’étape de la conception.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Les séquences complexes, répétitives ou palindromiques qui ne peuvent généralement pas être synthétisées sous forme de gènes peuvent être codées dans des oligonucléotides.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Les structures peptidiques plus importantes ou les architectures de génomes peuvent être codées dans des oligos de 300 nt de long.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>La synthèse uniforme minimise le suréchantillonnage nécessaire à un criblage complet.</span></span></span></span></span></span></li> </ul> <p><span><span><span><span><span><span><a href="/fr/blog/science/identifying-coronavirus-virulence-factors-rna-vaccine-development-twist-oligo-pools?topic=601&products=226"><img alt="Identification des facteurs de virulence du coronavirus pour le développement de vaccins à ARN" src="https://www.twistbioscience.com/sites/default/files/inline-images/Identifying-Coronavirus-Virulence-Factors-for-RNA-Vaccine-Development.png"></a></span></span></span></span></span></span></p> <p><span><span><span><span><span><span>Lisez notre article :<a href="/fr/blog/science/identifying-coronavirus-virulence-factors-rna-vaccine-development-twist-oligo-pools?topic=601&products=226"> </a></span></span></span></span></span></span><a class="green-link" href="/fr/blog/science/identifying-coronavirus-virulence-factors-rna-vaccine-development-twist-oligo-pools?topic=601&products=226">Identification des facteurs de virulence du coronavirus pour le développement de vaccins à ARN avec la méthode fate-seq basée sur le pool d’oligonucléotides de Twist Bioscience.</a></p></div> </div> <div class="clearfix component-paragraph with-divider paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Cribles de contrôle génétique à haut débit</h3> <p><span><span><span><span><span><span>La compréhension des éléments génétiques contrôlant l’expression et la régulation des gènes trouve une large application dans la biologie de synthèse et le développement de traitements potentiels des maladies. Les pool d’oligonucléotides permettent de réaliser des cribles à haut débit du contrôle génétique, notamment le profilage de l’expression génétique et les applications basées sur les rapporteurs. </span></span></span></span></span></span></p> <p><span><span><span><strong><span><span>Les avantages du produit sont les suivants :</span></span></strong></span></span></span></p> <ul> <li><span><span><span><span><span><span>Des pools de haute qualité permettent de vérifier la fonctionnalité de chaque base.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Une grande uniformité signifie que vous criblerez moins pour trouver vos réponses.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Des pools de 300 nt vous donnent la possibilité d’ajouter plusieurs éléments ou régions d’homologie.</span></span></span></span></span></span></li> </ul> <p><a href="/fr/resources/webinar/epitranscriptome-screening-technetworks"><img alt="Criblage épitranscriptomique sur TechNetworks" src="https://www.twistbioscience.com/sites/default/files/inline-images/Epitranscriptome-Screening-on-TechNetworks.png"></a></p> <p>Regardez notre webinaire : <a class="green-link" href="/fr/resources/webinar/epitranscriptome-screening-technetworks">Criblage épitranscriptomique sur TechNetworks</a></p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Hybridation in situ en fluorescence</h3> <p><span><span><span><span><span><span>L’hybridation in situ par fluorescence (FISH) permet de visualiser directement l’abondance et l’organisation spatiale des acides nucléiques dans les échantillons biologiques. Les pool d’oligonucléotides peuvent coder des sondes qui, lorsqu’elles sont liées à des marqueurs fluorescents, permettent une visualisation programmable et réglable d’un grand nombre de cibles.</span></span></span></span></span></span></p> <p><span><span><span><strong><span><span>Les avantages du produit sont les suivants :</span></span></strong></span></span></span></p> <ul> <li><span><span><span><span><span><span>Développement rapide de sondes FISH</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Le contrôle de la séquence permet un ciblage efficace</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Des oligonucléotides plus longs permettent un ciblage plus spécifique</span></span></span></span></span></span></li> </ul> <p><a href="/fr/resources/webinar/rapid-and-efficient-production-fish-probes-using-oligo-pools-nicola-crosetto"><img alt="Production rapide et efficace de sondes FISH à l’aide de pools d’oligonucléotides, avec Nicola Crosetto" src="https://www.twistbioscience.com/sites/default/files/inline-images/Rapid-and-efficient-production-of-FISH-probes-using-oligo-pools-with-Nicola-Crosetto_0.png"></a></p> <p>Regardez notre webinaire : <a class="green-link" href="/fr/resources/webinar/rapid-and-efficient-production-fish-probes-using-oligo-pools-nicola-crosetto">Production rapide et efficace de sondes FISH à l’aide de pools d’oligonucléotides, avec Nicola Crosetto</a></p></div> </div> </div> <div class="clearfix"></div> </div> </div> </div> <div class="mobile-layer"> <div class="clearfix component-paragraph with-divider paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Banques d’ARN guides pour les cribles CRISPR</h3> <p><span><span><span><span><span><span>Les cribles CRISPR comportent l’élimination, l’interférence ou la modulation précises et à haut débit de l’activité des gènes, ce qui permet de révéler des interactions génotype-phénotype complexes. Les pool d’oligonucléotides sont conçus pour coder plusieurs molécules d’ARN guide par gène.  </span></span></span></span></span></span></p> <p><span><span><span><strong><span><span>Les avantages du produit sont les suivants :</span></span></strong></span></span></span></p> <ul> <li><span><span><span><span><span><span>Un contrôle total sur la conception de la banque de guides, évitant les guides non désirés, le gaspillage de séquençage et les ratios limités de guides par gène.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>L’association d’enzymes Cas haute fidélité et d’une synthèse très précise pour des cribles haute fidélité avec des effets hors cible minimaux.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Des pools incroyablement uniformes et sans biais permettent d’obtenir un rapport signal/bruit amplifié pour une détection maximale des résultats, ce qui réduit les coûts en aval.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Les oligonucléotides allant jusqu’à 300 nt libèrent de l’espace pour des éléments expérimentaux supplémentaires. Il est facile d’ajouter des guides multiples par oligonucléotide, des homologies génomiques ou des promoteurs minimaux.</span></span></span></span></span></span></li> </ul> <p><a href="https://www.twistbioscience.com/fr/resources/webinar/scalable-and-combinatorial-single-cell-crispr-screens-direct-guide-rna-capture"><img alt="Next Generation CRISPR Screens" src="https://www.twistbioscience.com/sites/default/files/inline-images/Next-generation-CRISPR-screens-based-on-single-cell-transcriptomics-11.24_1.png"></a></p> <p><span><span><span><span><span><span><span><span>Assistez à notre webinaire : <a class="green-link" href="/fr/resources/webinar/scalable-and-combinatorial-single-cell-crispr-screens-direct-guide-rna-capture"> Criblages CRISPR unicellulaires évolutifs et combinatoires par capture d’ARN directe et séquençage ciblé</a></span></span></span></span></span></span></span></span></p> </div> </div> <div class="clearfix component-paragraph with-divider paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Banques de données génomiques relatives aux puces à sonde recouvrantes</h3> <p><span><span><span><span><span><span>Les banques de puces permettent une étude complète de la fonctionnalité génomique. Les pools d’oligonucléotides peuvent coder des jeux ordonnés de peptides, des séquences codant des ARNm ou des fragments de génome.</span></span></span></span></span></span></p> <p><span><span><span><strong><span><span>Les avantages du produit sont les suivants :</span></span></strong></span></span></span></p> <ul> <li><span><span><span><span><span><span>Le « tiling » détermine les régions génomiques d’intérêt. Réduire le bruit expérimental en excluant les séquences indésirables à l’étape de la conception.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Les séquences complexes, répétitives ou palindromiques qui ne peuvent généralement pas être synthétisées sous forme de gènes peuvent être codées dans des oligonucléotides.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Les structures peptidiques plus importantes ou les architectures de génomes peuvent être codées dans des oligos de 300 nt de long.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>La synthèse uniforme minimise le suréchantillonnage nécessaire à un criblage complet.</span></span></span></span></span></span></li> </ul> <p><span><span><span><span><span><span><a href="/fr/blog/science/identifying-coronavirus-virulence-factors-rna-vaccine-development-twist-oligo-pools?topic=601&products=226"><img alt="Identification des facteurs de virulence du coronavirus pour le développement de vaccins à ARN" src="https://www.twistbioscience.com/sites/default/files/inline-images/Identifying-Coronavirus-Virulence-Factors-for-RNA-Vaccine-Development.png"></a></span></span></span></span></span></span></p> <p><span><span><span><span><span><span>Lisez notre article :<a href="/fr/blog/science/identifying-coronavirus-virulence-factors-rna-vaccine-development-twist-oligo-pools?topic=601&products=226"> </a></span></span></span></span></span></span><a class="green-link" href="/fr/blog/science/identifying-coronavirus-virulence-factors-rna-vaccine-development-twist-oligo-pools?topic=601&products=226">Identification des facteurs de virulence du coronavirus pour le développement de vaccins à ARN avec la méthode fate-seq basée sur le pool d’oligonucléotides de Twist Bioscience.</a></p></div> </div> <div class="clearfix component-paragraph with-divider paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Cribles de contrôle génétique à haut débit</h3> <p><span><span><span><span><span><span>La compréhension des éléments génétiques contrôlant l’expression et la régulation des gènes trouve une large application dans la biologie de synthèse et le développement de traitements potentiels des maladies. Les pool d’oligonucléotides permettent de réaliser des cribles à haut débit du contrôle génétique, notamment le profilage de l’expression génétique et les applications basées sur les rapporteurs. </span></span></span></span></span></span></p> <p><span><span><span><strong><span><span>Les avantages du produit sont les suivants :</span></span></strong></span></span></span></p> <ul> <li><span><span><span><span><span><span>Des pools de haute qualité permettent de vérifier la fonctionnalité de chaque base.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Une grande uniformité signifie que vous criblerez moins pour trouver vos réponses.</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Des pools de 300 nt vous donnent la possibilité d’ajouter plusieurs éléments ou régions d’homologie.</span></span></span></span></span></span></li> </ul> <p><a href="/fr/resources/webinar/epitranscriptome-screening-technetworks"><img alt="Criblage épitranscriptomique sur TechNetworks" src="https://www.twistbioscience.com/sites/default/files/inline-images/Epitranscriptome-Screening-on-TechNetworks.png"></a></p> <p>Regardez notre webinaire : <a class="green-link" href="/fr/resources/webinar/epitranscriptome-screening-technetworks">Criblage épitranscriptomique sur TechNetworks</a></p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-text paragraph--view-mode--default"> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><h3>Hybridation in situ en fluorescence</h3> <p><span><span><span><span><span><span>L’hybridation in situ par fluorescence (FISH) permet de visualiser directement l’abondance et l’organisation spatiale des acides nucléiques dans les échantillons biologiques. Les pool d’oligonucléotides peuvent coder des sondes qui, lorsqu’elles sont liées à des marqueurs fluorescents, permettent une visualisation programmable et réglable d’un grand nombre de cibles.</span></span></span></span></span></span></p> <p><span><span><span><strong><span><span>Les avantages du produit sont les suivants :</span></span></strong></span></span></span></p> <ul> <li><span><span><span><span><span><span>Développement rapide de sondes FISH</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Le contrôle de la séquence permet un ciblage efficace</span></span></span></span></span></span></li> <li><span><span><span><span><span><span>Des oligonucléotides plus longs permettent un ciblage plus spécifique</span></span></span></span></span></span></li> </ul> <p><a href="/fr/resources/webinar/rapid-and-efficient-production-fish-probes-using-oligo-pools-nicola-crosetto"><img alt="Production rapide et efficace de sondes FISH à l’aide de pools d’oligonucléotides, avec Nicola Crosetto" src="https://www.twistbioscience.com/sites/default/files/inline-images/Rapid-and-efficient-production-of-FISH-probes-using-oligo-pools-with-Nicola-Crosetto_0.png"></a></p> <p>Regardez notre webinaire : <a class="green-link" href="/fr/resources/webinar/rapid-and-efficient-production-fish-probes-using-oligo-pools-nicola-crosetto">Production rapide et efficace de sondes FISH à l’aide de pools d’oligonucléotides, avec Nicola Crosetto</a></p></div> </div> </div> </div> </div> </div> <div class="field__item select-picker-4" data-id="4"> <div class="no-custom-show-more paragraph paragraph--type--section-tabs paragraph--view-mode--default array" data-style="array"> <div class="field field--name-field-cp-title field--type-string field--label-hidden field__item" id="resources">Ressources</div> <div class="field field--name-field-cp-paragraphs field--type-entity-reference-revisions field--label-hidden field__items"> <div class="desktop-layer"> <div class="container"> <div class="virtual-wrapper row"> <div class="virtual-region-left col-md-12"> <div class="component_link_accordion accordion accordion-flush" id="accordionFlushExample"><div class="accordion-item"><div class="accordion-header accordion-button collapsed" id="flush-406" data-bs-toggle="collapse" data-bs-target="#flush-collapse-406" aria-expanded="false" aria-controls="flush-collapse-406"><img src="https://www.twistbioscience.com/fr/sites/default/files/2019-06/dark-datasheet-slate.svg" alt="" class="me-2" />Fiche produit</div><div id="flush-collapse-406" class="accordion-collapse collapse" aria-labelledby="flush-406" data-bs-parent="#accordionFlushExample"><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resource/twist-oligo-pools" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/406" id="taxonomy-term-406" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Fiche produit</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2019-06/dark-datasheet-slate.svg" alt="Fiche produit" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Twist Oligo Pools</p></div> </div> </div><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/product-sheet/twist-cloned-oligo-pools" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/406" id="taxonomy-term-406" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Fiche produit</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2019-06/dark-datasheet-slate.svg" alt="Fiche produit" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Pools d’oligonucléotides clonés Twist</p></div> </div> </div></div></div><div class="accordion-item"><div class="accordion-header accordion-button collapsed" id="flush-426" data-bs-toggle="collapse" data-bs-target="#flush-collapse-426" aria-expanded="false" aria-controls="flush-collapse-426"><img src="https://www.twistbioscience.com/fr/sites/default/files/2020-08/dark-protocol-slate.svg" alt="" class="me-2" />Protocole</div><div id="flush-collapse-426" class="accordion-collapse collapse" aria-labelledby="flush-426" data-bs-parent="#accordionFlushExample"><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/op_protocol_ampguidelines" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/426" id="taxonomy-term-426" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Protocole</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-protocol-slate.svg" alt="Protocole" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Guide d’amplification des pool d’oligonucléotides</p></div> </div> </div><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="https://www.twistbioscience.com/fr/resources/protocol/twist-dna-resuspension-guidelines" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/426" id="taxonomy-term-426" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Protocole</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-protocol-slate.svg" alt="Protocole" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="field field--name-field-cp-link-subtitle field--type-string field--label-hidden field__item">Directives sur la remise en suspension d’ADN de Twist</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Ces directives décrivent les meilleures pratiques pour remettre en suspension les fragments de gènes Twist, les gènes clonaux, les pools d’oligonucléotides et les banques de variants.</p></div> </div> </div></div></div><div class="accordion-item"><div class="accordion-header accordion-button collapsed" id="flush-396" data-bs-toggle="collapse" data-bs-target="#flush-collapse-396" aria-expanded="false" aria-controls="flush-collapse-396"><img src="https://www.twistbioscience.com/fr/sites/default/files/2020-08/dark-casestudy-slate.svg" alt="" class="me-2" />Étude de cas</div><div id="flush-collapse-396" class="accordion-collapse collapse" aria-labelledby="flush-396" data-bs-parent="#accordionFlushExample"><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/case-study/baker-lab-university-washington-designing-proteins-revolutionize-biotechnology" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/396" id="taxonomy-term-396" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Étude de cas</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-casestudy-slate.svg" alt="Étude de cas" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Conception de protéines pour révolutionner la biotechnologie</p></div> </div> </div></div></div><div class="accordion-item"><div class="accordion-header accordion-button collapsed" id="flush-436" data-bs-toggle="collapse" data-bs-target="#flush-collapse-436" aria-expanded="false" aria-controls="flush-collapse-436"><img src="https://www.twistbioscience.com/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="" class="me-2" />Webinaire</div><div id="flush-collapse-436" class="accordion-collapse collapse" aria-labelledby="flush-436" data-bs-parent="#accordionFlushExample"><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/next-generation-crispr-screens-based-single-cell-transcriptomics" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Criblages CRISPR de nouvelle génération basés sur la transcriptomique en cellule unique</p></div> </div> </div><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/using-amino-acid-composition-and-conservation-more-efficient-crispr-screens" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Utilisation de la composition et de la conservation des acides aminés pour des criblages CRISPR plus efficaces</p></div> </div> </div><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/rapid-and-efficient-production-fish-probes-using-oligo-pools-nicola-crosetto" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Production rapide et efficace de sondes FISH à l’aide de pools d’oligonucléotides</p></div> </div> </div><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/epitranscriptome-screening-technetworks" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Criblage de l’épitranscriptome</p></div> </div> </div><div class="accordion-body"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/crispr-cas9-screening-human-pluripotent-stem-cells" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden 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href="/fr/resources/webinar/discovering-parp-inhibitor-resistance-mechanisms-using-genome-wide-and-focused" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Recherche des mécanismes de résistance de l’inhibiteur de PARP à l’aide de criblages CRISPR focalisés et à l’échelle du génome</p></div> </div> </div></div></div></div> </div> <div class="clearfix"></div> </div> </div> </div> <div class="mobile-layer"> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resource/twist-oligo-pools" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/406" id="taxonomy-term-406" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Fiche produit</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2019-06/dark-datasheet-slate.svg" alt="Fiche produit" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Twist Oligo Pools</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/product-sheet/twist-cloned-oligo-pools" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/406" id="taxonomy-term-406" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Fiche produit</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2019-06/dark-datasheet-slate.svg" alt="Fiche produit" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Pools d’oligonucléotides clonés Twist</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/op_protocol_ampguidelines" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/426" id="taxonomy-term-426" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Protocole</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-protocol-slate.svg" alt="Protocole" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Guide d’amplification des pool d’oligonucléotides</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/case-study/baker-lab-university-washington-designing-proteins-revolutionize-biotechnology" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/396" id="taxonomy-term-396" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Étude de cas</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-casestudy-slate.svg" alt="Étude de cas" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Conception de protéines pour révolutionner la biotechnologie</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/next-generation-crispr-screens-based-single-cell-transcriptomics" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Criblages CRISPR de nouvelle génération basés sur la transcriptomique en cellule unique</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/using-amino-acid-composition-and-conservation-more-efficient-crispr-screens" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Utilisation de la composition et de la conservation des acides aminés pour des criblages CRISPR plus efficaces</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/rapid-and-efficient-production-fish-probes-using-oligo-pools-nicola-crosetto" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Production rapide et efficace de sondes FISH à l’aide de pools d’oligonucléotides</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/epitranscriptome-screening-technetworks" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Criblage de l’épitranscriptome</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/crispr-cas9-screening-human-pluripotent-stem-cells" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Criblage CRISPR-Cas9 dans des cellules souches humaines pluripotentes</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="/fr/resources/webinar/discovering-parp-inhibitor-resistance-mechanisms-using-genome-wide-and-focused" target="_blank">À REGARDER</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/436" id="taxonomy-term-436" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Webinaire</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-webinar-slate.svg" alt="Webinaire" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Recherche des mécanismes de résistance de l’inhibiteur de PARP à l’aide de criblages CRISPR focalisés et à l’échelle du génome</p></div> </div> <div class="clearfix component-paragraph paragraph paragraph--type--component-link paragraph--view-mode--default"> <div class="field field--name-field-cp-link-link field--type-link field--label-hidden field__item"><a href="https://www.twistbioscience.com/fr/resources/protocol/twist-dna-resuspension-guidelines" target="_blank">À LIRE</a></div> <div class="field field--name-field-cp-link-resource-type field--type-entity-reference field--label-hidden field__item"><div about="/taxonomy/term/426" id="taxonomy-term-426" class="taxonomy-term vocabulary-resource-types"> <h2> <div class="field field--name-name field--type-string field--label-hidden field__item">Protocole</div> </h2> <div class="content"> <div class="field field--name-field-resource-icon-dark field--type-image field--label-hidden field__item"> <img src="/fr/sites/default/files/2020-08/dark-protocol-slate.svg" alt="Protocole" loading="lazy" typeof="foaf:Image" /> </div> </div> </div> </div> <div class="field field--name-field-cp-link-subtitle field--type-string field--label-hidden field__item">Directives sur la remise en suspension d’ADN de Twist</div> <div class="custom-show-more clearfix text-formatted field field--name-field-cp-text-text field--type-text-with-summary field--label-hidden field__item"><p>Ces directives décrivent les meilleures pratiques pour remettre en suspension les fragments de gènes Twist, les gènes clonaux, les pools d’oligonucléotides et les banques de variants.</p></div> </div> </div> </div> </div> </div> </div> <div class="field field--name-field-components field--type-entity-reference-revisions field--label-hidden field__items"> <div class="field__item"> <section class="section-background" style="background-color:#ECF9FA;"> <div class="clearfix component-paragraph paragraph paragraph--type--section paragraph--view-mode--default"> <div class="section-wrapper container"> <div class="row"> <div class="paragraph paragraph--type--component-testimonials paragraph--view-mode--default col-lg-12 col-sm-12 col-12"> <h2 class="cp-title">Ce que les scientifiques ont à dire</h2> <div class="testimonials-wrapper-slick clearfix"> <div class="sub-block sub-block-0"> <span class="testimonial-quote" style="color: #19805E">« </span> <div class="ti-quote">Les pools d’oligonucléotides d’ADNss de Twist sont bien adaptés à la fabrication de structures d’ARN groupées en raison de leur grande uniformité, de leur faible taux d’erreur et de leurs longueurs allant jusqu’à 300 nt. Ces caractéristiques permettent de cribler les interactions ARN-petites molécules avec une précision et une efficacité élevées.</div> <div class="bottom-data"> <div class="ti-img"></div> <div class="ti-name">Kaoru R. Komatsu</div> <div class="ti-institution">Directeur délégué et Directeur technique • xFOREST Therapeutics Co., Ltd</div> </div> </div> <div class="sub-block sub-block-1"> <span class="testimonial-quote" style="color: #19805E">« </span> <div class="ti-quote">Nous avons trouvé dans les pool d’oligonucléotides de Twist Bioscience un moyen rapide, pratique et flexible de synthétiser des banques CRISPR personnalisées de haute qualité pour nos recherches.</div> <div class="bottom-data"> <div class="ti-img"></div> <div class="ti-name">Stephen Pettit</div> <div class="ti-institution">Chercheur • Institute of Cancer Research, R-U</div> </div> </div> <div class="sub-block sub-block-2"> <span class="testimonial-quote" style="color: #19805E">« </span> <div class="ti-quote">Nous travaillons sur des analyses de rapporteurs massivement parallèles (massively parallel reporter assays, MPRA) axées sur l’identification des fonctions de régulation des gènes par des substitutions d’une seule base, de sorte qu’une haute fidélité des oligonucléotides est essentielle. Le séquençage du pool que nous avons obtenu de Twist a été d’une qualité stupéfiante, avec deux fois moins de mutations que ce à quoi nous nous attendions.</div> <div class="bottom-data"> <div class="ti-img"></div> <div class="ti-name">Bernie Mulvey</div> <div class="ti-institution">Doctorant en médecine - École de médecine de l’Université de Washington - St. Louis, MO, États-Unis</div> </div> </div> </div> </div> </div> </div> </div> </section> </div> </div> <div class="header-right-container"> <div class="field field--name-field-pc-tag-line field--type-entity-reference-revisions field--label-hidden field__item"> <div class="paragraph paragraph--type--component-tagline paragraph--view-mode--default"> <div class="clearfix text-formatted field field--name-field-cp-mobile-text field--type-text field--label-hidden field__item">Performance exceptionnelle quelle que soit l’échelle</div> <div class="clearfix text-formatted field field--name-field-cp-tablet-text field--type-text field--label-hidden field__item">Performance exceptionnelle quelle que soit l’échelle</div> <div class="clearfix text-formatted field field--name-field-cp-desktop-text field--type-text field--label-hidden field__item">Performance exceptionnelle quelle que soit l’échelle</div> </div> </div> <div class="field field--name-field-pc-link-button field--type-link field--label-hidden field__item"><a href="https://ecommerce.twistdna.com/app/oligo/" id="order_now_oligo">COMMANDER MAINTENANT</a></div> </div> </div> </article> </div> </div> <div id="block-twist-barrio-videomodule" class="block-content-paragraphs_block block block-block-content block-block-content85916772-3905-4bc7-9766-0d59b7f7d595"> <div class="content"> <div class="field field--name-field-paragraph-section field--type-entity-reference-revisions field--label-hidden field__items"> <div class="field__item"> <section class="section-background pt5 pb6 ecommerce invert" style="background-color:#293E36;"> <div class="clearfix component-paragraph paragraph paragraph--type--section paragraph--view-mode--default"> <div class="section-wrapper container"> <div class="row"> <div class="paragraph col-lg-12 col-sm-12 col-12 paragraph--type--text paragraph--view-mode--default"> <div class="tile " style="background-color: transparent;"> <div class="clearfix text-formatted field field--name-field-paragraph-text field--type-text-long field--label-hidden field__item"><h3 class="text-align-center">Enfin un processus simple de commande d’ADN </h3></div> </div> </div> <div class="paragraph col-lg-12 col-sm-12 col-12 paragraph--type--html paragraph--view-mode--default"> <div style="background-color:transparent"> <div class="clearfix text-formatted field field--name-field-html field--type-text-long field--label-hidden field__item"><script src="https://fast.wistia.com/embed/medias/bxsgmnx5ug.jsonp" async></script><script src="https://fast.wistia.com/assets/external/E-v1.js" async></script><div class="wistia_responsive_padding" style="padding:56.25% 0 0 0;position:relative;"> <div class="wistia_responsive_wrapper" style="height:100%;left:0;position:absolute;top:0;width:100%;"> <div class="wistia_embed wistia_async_bxsgmnx5ug seo=false videoFoam=true" style="height:100%;width:100%"> </div> </div> </div> <style type="text/css">.page-node-type-product-parent .ecommerce .paragraph--type--icon-with-text .field--name-field-html { color:#ffffff !important; 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Pools d’oligonucléotides pour les cribles à haut débit - Twist Bioscience