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This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.<br /><span class="u-fw700">Phone: </span>+9821-22510895 , +9821-23562175<br /><b>Address: </b>5th Floor, No 9, Royan Institute Cell Therapy Center, East Shaghayegh Alley, Bani Hashem Sq, Bani Hashem St, Resalat Highway, Tehran, Iran.<br />P.O.Box: 16635-148,Tehran ,Iran<br /><div class="js-profile-less-about u-linkUnstyled u-tcGrayDarker u-textDecorationUnderline u-displayNone">less</div></div></div><div class="suggested-academics-container"><div class="suggested-academics--header"><p class="ds2-5-body-md-bold">Related Authors</p></div><ul class="suggested-user-card-list"><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://tums.academia.edu/GholamrezaZamani"><img 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href="https://www.academia.edu/127680381/Cell_Based_Therapy_for_Cerebral_Palsy_A_Puzzle_in_Progress"><img alt="Research paper thumbnail of Cell-Based Therapy for Cerebral Palsy: A Puzzle in Progress" class="work-thumbnail" src="https://attachments.academia-assets.com/121377935/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680381/Cell_Based_Therapy_for_Cerebral_Palsy_A_Puzzle_in_Progress">Cell-Based Therapy for Cerebral Palsy: A Puzzle in Progress</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 9, September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cell-based therapy has shown promising outcomes in the treatment of cerebral palsy (CP). However,...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cell-based therapy has shown promising outcomes in the treatment of cerebral palsy (CP). However, there is no consensus on a standard therapeutic protocol regarding the source of cells, optimal cell dose, timing and frequency of cell injections, route of administration, or the use of combination therapy. This lack of consensus necessitates a comprehensive investigation to clarify these crucial yet undefined factors in cell-based therapy for CP patients. In this commentary, we discuss and compare the trends in Gross Motor Function Measure-66 following intrathecal injection of umbilical cord blood mononuclear cells (UCB-MNCs) and umbilical cord tissue mesenchymal stromal cells (UCT-MSCs) in children with CP. Our study revealed that MNC injections led to earlier improvements in gross motor function, whereas MSC applications resulted in more sustainable changes. These findings provide key insights into the efficacy of different cell types, which will be beneficial for future studies and for refining cell-based therapy protocols for CP treatment.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f4392af34cd9945f4b29991691d11029" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377935,"asset_id":127680381,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377935/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680381"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680381"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680381; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680370"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680370/The_Role_of_Quercetin_and_Exercise_in_Modulating_Apoptosis_and_Cardiomyopathy_via_PI3K_AKT_FOXO3_Pathways_in_Diabetic_Obese_Rats"><img alt="Research paper thumbnail of The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats" class="work-thumbnail" src="https://attachments.academia-assets.com/121377924/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680370/The_Role_of_Quercetin_and_Exercise_in_Modulating_Apoptosis_and_Cardiomyopathy_via_PI3K_AKT_FOXO3_Pathways_in_Diabetic_Obese_Rats">The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 9, September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Quercetin and exercise both have antidiabetic effects through decreasing blood glucose...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Quercetin and exercise both have antidiabetic effects through decreasing blood glucose while increasing insulin sensitivity. Therefore, the present study aimed to investigate the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) exercises along with quercetin administration on apoptosis and cardiomyopathy in diabetic obese rats. Materials and Methods: In this experimental study, 35 male Wistar rats [diabetic rats for experimental groups and normal rats for healthy control (HC)] were divided into seven groups (for each group n=5): HC, diabetic control (DC), diabetic quercetin control (DQC), diabetic HIIT (DHT), diabetic MICT (DMT), DHT with quercetin (DQHT) and DMT with quercetin (DQMT). The rats were fed a high-fat diet (HFD) for 8 weeks and a low dose of streptozotocin (STZ) was administered to create a model of type 2 diabetes mellitus (T2DM). Eight weeks of HIIT and MICT with or without quercetin treatment were performed. Quercetin was used at 15 mg/kg, as a suspension in carboxymethyl cellulose (CMC) at a concentration of 0.5%. One-way analysis of variance with LSD's post-hoc test with a significant level of P≤0.05 was used to analyze data. Results: Just 8 weeks of HIIT and MICT protected the protein content of PI3K, AKT, and FOXO3 and caspase-8 (Casp-8) gene expression in heart tissues (P<0.05). Quercetin and both training protocols decreased blood glucose, while improving inflammatory markers and the lipid profile (P<0.05). Conclusion: Reduction in blood glucose along with improvements in the inflammatory markers and the lipid profile by quercetin injection may be a promising approach for the development of new antidiabetic medications. In addition, both training protocols showed potentially successful diabetic cardiomyopathy treatments through modulating the FOXO3 and PI3K/AKT pathways.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dbb9021d68833365a24ed2185d94d499" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377924,"asset_id":127680370,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377924/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680370"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680370"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680370; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680346"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680346/Interleukin_12_Inhibits_Tumor_Growth_and_Metastasis_Promoted_by_Tumor_Associated_Mesenchymal_Stem_Cells_in_Triple_Negative_Breast_Cancer"><img alt="Research paper thumbnail of Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer" class="work-thumbnail" src="https://attachments.academia-assets.com/121377905/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680346/Interleukin_12_Inhibits_Tumor_Growth_and_Metastasis_Promoted_by_Tumor_Associated_Mesenchymal_Stem_Cells_in_Triple_Negative_Breast_Cancer">Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 9, September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: The aim of this study was to understand the interactions between tumor-associated mese...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: The aim of this study was to understand the interactions between tumor-associated mesenchymal stem cells (TA-MSCs) and triple-negative breast cancer (TNBC) cells, which appear to be necessary for developing effective therapies. Materials and Methods: In this experimental study, MDA-MB-231 and 4T1 TNBC cells were co-cultured with bone marrow-derived MSCs, and TA-MSCs conditioned media (CM) were collected. TA-MSC CM-treated TNBC cells were subjected to migration and invasion assays. Epithelial-mesenchymal transition (EMT) marker expression was quantified by real-time polymerase chain reaction (RT-PCR). Cell proliferation was measured using trypan blue exclusion technique, while cell cycle distribution and apoptosis were assessed by flow cytometry. The effects of TA-MSCs on tumor volume, survival rate, and lung metastasis were evaluated by subcutaneous co-injection of MSCs with 4T1 cells in the right flanks of BALB/c mice (n=5 per group). Intratumoral interleukin-12 (IL-12) immunotherapy was performed using lentiviral particles as a rescue experiment. The TA-MSCs RNA-seq dataset (PRJEB27694) was analyzed to detect elevated metastasis-associated oncogenes, downloaded from the European Nucleotide Archive database. For validation of the RNA-seq data analysis, the expression levels of candidate oncogenes were evaluated in TA-MSCs, TNBC cells, and tumor tissue using RT-PCR. Results: TA-MSCs enhanced migration, invasion, and EMT of TNBC cells in vitro without affecting cell proliferation or apoptosis. In vivo, TA-MSCs increased tumor growth and lung metastasis, while decreasing survival rates. IL-12 therapy elevated serum IL-12 and interferon-gamma (IFN-γ) expression, suppressed tumor volume and lung metastasis, and improved overall survival in the TA-MSC group. RNA-seq data analysis identified upregulated oncogenes in TA-MSCs, among which MMP3, CXCL2, CXCL5, and ICAM1 were selected as the most relevant to metastasis. These genes showed increased expression in TA-MSCs, TNBC cells, and tumor tissues. Conclusion: The findings of the present study revealed a complex interplay between TA-MSCs and TNBC cells that affects tumor growth and metastasis. Preclinical results indicate that intratumoral IL-12 immunotherapy shows promise in overcoming TA-MSC-promoted tumor growth and metastasis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3b01d4214d35ef6f8609a763c2e2fad8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377905,"asset_id":127680346,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377905/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680346"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680346"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680346; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680324"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680324/Oxaliplatin_Loaded_Chitosan_Nanoparticles_Decorated_with_Cetuximab_Single_Chain_Variable_Fragment_for_Human_Colorectal_Cancer_Treatment"><img alt="Research paper thumbnail of Oxaliplatin-Loaded Chitosan Nanoparticles Decorated with Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment" class="work-thumbnail" src="https://attachments.academia-assets.com/121377880/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680324/Oxaliplatin_Loaded_Chitosan_Nanoparticles_Decorated_with_Cetuximab_Single_Chain_Variable_Fragment_for_Human_Colorectal_Cancer_Treatment">Oxaliplatin-Loaded Chitosan Nanoparticles Decorated with Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment</a></div><div class="wp-workCard_item"><span> Volume 26, Issue 9 , September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide. Engineered biomolecules can be used as a targeted tool to deliver drugs directly to tumors that reduce the adverse effects of conventional treatments. We aimed to prepare non-targeted oxaliplatin-loaded chitosan nanoparticles (OXPT-CS NPs) and targeted OXPT-CS NPs decorated with cetuximab single-chain variable fragment (scFv) to send both NPs to epidermal growth factor receptor (EGFR) overexpressing HCT 116 cells, a human colorectal carcinoma cell line, for comparing their cytotoxicity. Materials and Methods: In this experimental study, OXPT-CS NPs were synthesized using a fluid system. Encapsulation efficiency percentage (EE%) and oxaliplatin release rate were evaluated. Western blot and cell-based ELISA confirmed scFv production and its binding ability to EGFR, respectively. The Fourier transform infrared spectroscopy (FTIR) determined the conjugation of scFv to OXPT-CS NPs. The NPs were characterized, and their toxicity against the HCT 116 cells was evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and flow cytometry assays. Results: The EE% of OXPT-CS NPs was 93%, and the average diameters were 75.85 ± 8.81 nm and 92.48 ± 9.51 before and after scFv conjugation, respectively. The scFv was purified via affinity chromatography. The western blot method and cell-based ELISA revealed successful purification of scFv and its attachment to EGFR on HCT 116 cells. The FTIR analysis determined the interactions between the scFv and OXPT-CS NPs. According to MTT and flow cytometry results, the targeted delivery system significantly reduced HCT 116 cancer cell viability and increased apoptosis induction up to 99.8%. Conclusion: The scFv-OXPT-CS NPs demonstrated an increased cytotoxic function due to the presence of scFv in its formulation. This delivery system offers a promising method for delivering chemotherapy drugs to cancer cells. More research is needed on the best strategies for improving treatment efficacy by targeting cancer cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2098d1f55b4f71e4f0eda9ca0dbec763" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377880,"asset_id":127680324,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377880/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680324"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680324"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680324; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680324]").text(description); $(".js-view-count[data-work-id=127680324]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680324; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680324']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2098d1f55b4f71e4f0eda9ca0dbec763" } } $('.js-work-strip[data-work-id=127680324]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680324,"title":"Oxaliplatin-Loaded Chitosan Nanoparticles Decorated with Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment","internal_url":"https://www.academia.edu/127680324/Oxaliplatin_Loaded_Chitosan_Nanoparticles_Decorated_with_Cetuximab_Single_Chain_Variable_Fragment_for_Human_Colorectal_Cancer_Treatment","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377880,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377880/thumbnails/1.jpg","file_name":"Cell_J_26_530.pdf","download_url":"https://www.academia.edu/attachments/121377880/download_file","bulk_download_file_name":"Oxaliplatin_Loaded_Chitosan_Nanoparticle.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377880/Cell_J_26_530-libre.pdf?1739687738=\u0026response-content-disposition=attachment%3B+filename%3DOxaliplatin_Loaded_Chitosan_Nanoparticle.pdf\u0026Expires=1739699916\u0026Signature=abbbkqNFBPLtBp1R-LtzZoUvrVtbZGqePrOXzjmOq-szAdrgYEVCkuRmOP3KzYGr9XmIZLkJjt75XNMVuXte0Nv4Rwr3uBNSc1aTxs4P~-JcFetSZKQswxU5uTit3-cOOUUyXB3d5LWG75og3HUYgAmj~i6dikos-jdYhZe5NaZd3w6H6vv87lYRwp1x1gPJ22L8~jkbsCsVSdM3aMozQIT1yX7cdEPQe1m2mgA6zSwIuulHpw3jC8TxIX-xveJWdRyPr6AWilry72F9IrPiAa1EsFNnYBvUUKMCEo1SzSo4mg0HbrSfjPvtDg6dqIpdf4kxk5dd76D9ZfQXhsC4GA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680291"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680291/The_Geographical_Distribution_of_Global_Biobanks"><img alt="Research paper thumbnail of The Geographical Distribution of Global Biobanks" class="work-thumbnail" src="https://attachments.academia-assets.com/121377846/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680291/The_Geographical_Distribution_of_Global_Biobanks">The Geographical Distribution of Global Biobanks</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 9, September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This study aimed to comprehensively review the global biobanks to visualize their geographical di...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This study aimed to comprehensively review the global biobanks to visualize their geographical distribution. The protocol for this review consisted of the following steps: i. Developing a search strategy to identify biobanks from each continent, ii. Defining variables (such as tissue-based, cell-based, and gene-based biobanks) and organizing them in Excel sheets for data collection, iii. Collecting data, iv. Removing duplicate and invalid entries, v. Structuring the database, and vi. Analyzing the data. MATLAB software was utilized for data analysis and chart plotting. Data on global biobanks aimed to collected through targeted searches of databases, publications, and registries using predefined variables such as biobank type, location, and accessibility. The data were organized, cleaned to remove duplicates, and analyzed using MATLAB to visualize geographical distribution and prevalence patterns. Tissue and cell-based, tissue-based, and cellbased biobanks were the most common type of global biobanks with a prevalence of 30.4, 27.93, and 25.15%. United Kingdom (n=78, P=43.09%), Canada (n=43, P=23.75%), and the United States (n=33, P=18.23%) were the countries with a higher frequency of tissue-based biobanks (domain frequency: 1-78; 0.55-43.09%). However, tissue and genebased biobanks had the most minor frequency and were only in two countries of Spain (n=1, P=25%) and the United Kingdom (n=3, P=75%). The results of this study indicate that the feasibility of designing and conducting biobanks varies by type. Tissue and cell-based biobanks were found to be more prevalent, followed by tissue-based, cell-based, cell and gene-based, tissue, cell, and gene-based, gene-based, and finally, tissue and gene-based biobanks. This study represents the initial step in creating a global database by identifying all types of biobanks worldwide.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8f5ff159a07dd65036d206dcdfebb7bb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377846,"asset_id":127680291,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377846/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680291"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680291"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680291; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680291]").text(description); $(".js-view-count[data-work-id=127680291]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680291; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680291']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8f5ff159a07dd65036d206dcdfebb7bb" } } $('.js-work-strip[data-work-id=127680291]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680291,"title":"The Geographical Distribution of Global Biobanks","internal_url":"https://www.academia.edu/127680291/The_Geographical_Distribution_of_Global_Biobanks","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377846,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377846/thumbnails/1.jpg","file_name":"Cell_J_26_523.pdf","download_url":"https://www.academia.edu/attachments/121377846/download_file","bulk_download_file_name":"The_Geographical_Distribution_of_Global.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377846/Cell_J_26_523-libre.pdf?1739687731=\u0026response-content-disposition=attachment%3B+filename%3DThe_Geographical_Distribution_of_Global.pdf\u0026Expires=1740152616\u0026Signature=aZV16RM2eOQn8esajtAevApYRQB3KArVshU7ERnaR0g2yJfmg1NmK5F13~6TnrRLRcQOZeTx-ysu0SXJ8ZlXMCrYsK3W~PN9lx9pNL09S1bCFooGfubkYQAxcqRwu~2ITU~~Uq5pEikmjAVIKKKCp~dd9~26-ZC3AC3D9Ak-XVhVfjfMDtQCSdHpZJT46GceCX5tCNeXqVBoTVFQRe3f2QsU4F0YLRG-2AE9DLTxoIrheT7VYGeTE1dvf6uTmS8lYzK3me6zUwJXVeMpKfTPlicTyCEqB9M07rkpCI~h7mDpIaFvYNE3HfDG5lkhBW2B81XmCJsytZQm2lZx4Gh84g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680193"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680193/Uncovering_Deletion_Insertion_Mutations_in_Veno_Occlusive_Disease_with_Immunodeficiency_Syndrome_in_An_Iranian_Family_A_Case_Report"><img alt="Research paper thumbnail of Uncovering Deletion/Insertion Mutations in Veno-Occlusive Disease with Immunodeficiency Syndrome in An Iranian Family: A Case Report" class="work-thumbnail" src="https://attachments.academia-assets.com/121377766/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680193/Uncovering_Deletion_Insertion_Mutations_in_Veno_Occlusive_Disease_with_Immunodeficiency_Syndrome_in_An_Iranian_Family_A_Case_Report">Uncovering Deletion/Insertion Mutations in Veno-Occlusive Disease with Immunodeficiency Syndrome in An Iranian Family: A Case Report</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Veno-occlusive disease with immunodeficiency (VODI) syndrome is a rare genetic disorder character...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Veno-occlusive disease with immunodeficiency (VODI) syndrome is a rare genetic disorder characterized by immune system irregularities and a significant mortality rate, despite its infrequency. SP110, situated on chromosome 2q37.1, plays a pivotal role in VODI syndrome, and its association with tuberculosis has been extensively studied. The identification of SP110 mutations holds promise for accelerating the diagnosis and treatment of VODI syndrome, by providing a comprehensive panel for diagnosis and potentially leading to targeted therapies. In this case study, we examined a three-year-old girl born to a consanguineous union who was suspected of having an immunodeficiency disorder. Whole-exome sequencing (WES) and clinical assessments were conducted to screen for and confirm potentially pathogenic mutations. The detected mutation was further analyzed using bioinformatics tools to forecast its impact on protein structure. WES analysis revealed a novel deletion-insertion mutation, c.1181-1182delAGinsT, within SP110. Protein analysis indicated substantial structural modifications in the SP110 protein. This study identified a novel deletion-insertion mutation as a potential contributor to VODI syndrome by affecting the functionality of the SP110 protein. By including various mutations associated with the SP110 gene, this study aimed to expedite diagnosis by creating a comprehensive panel for VODI syndrome.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7553e60a8771bad8bf2e1355523957f1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377766,"asset_id":127680193,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377766/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680193"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680193"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680193; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680193]").text(description); $(".js-view-count[data-work-id=127680193]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680193; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680193']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7553e60a8771bad8bf2e1355523957f1" } } $('.js-work-strip[data-work-id=127680193]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680193,"title":"Uncovering Deletion/Insertion Mutations in Veno-Occlusive Disease with Immunodeficiency Syndrome in An Iranian Family: A Case Report","internal_url":"https://www.academia.edu/127680193/Uncovering_Deletion_Insertion_Mutations_in_Veno_Occlusive_Disease_with_Immunodeficiency_Syndrome_in_An_Iranian_Family_A_Case_Report","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377766,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377766/thumbnails/1.jpg","file_name":"Cell_J_26_515.pdf","download_url":"https://www.academia.edu/attachments/121377766/download_file","bulk_download_file_name":"Uncovering_Deletion_Insertion_Mutations.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377766/Cell_J_26_515-libre.pdf?1739687767=\u0026response-content-disposition=attachment%3B+filename%3DUncovering_Deletion_Insertion_Mutations.pdf\u0026Expires=1740213530\u0026Signature=OCpEYj-8SfKky4IQg~aPpMnfTA1dx6IbNZtLu-iv9qHE5CPWQZqMY6r7SJk71SUFlQxBa~49mnHzAak202YriqZj9OrGZrSootJgXtAUyMV8Dk9hVZa0SAIuNlTrzTEOAsRiF97X9po9eY8Xm3R3k1Fps1GrUYiU6iFYVIbSwA4Z980C6WDPNjsmInfv8UPyt05hSD2OCPwpLwJnud5vnE-oyxie12-hXvmd9H-DHkCBhcaf~UUQlBfbJ8~IlluRlmiLbYEv-k84mLCItSq9aFBsEnHbCubtQxfnv3x013Fg-3hkVKsM~Rw5gdzrg9C~IeNEmDmYlbhbQc4A-AM-9Q__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680183"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680183/Application_of_XBP1s_Decoy_Oligodeoxynucleotide_Attenuates_Cancerous_Phenotype_in_Huh_7_Hepatocellular_Carcinoma_Cells"><img alt="Research paper thumbnail of Application of XBP1s Decoy Oligodeoxynucleotide Attenuates Cancerous Phenotype in Huh-7 Hepatocellular Carcinoma Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/121377711/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680183/Application_of_XBP1s_Decoy_Oligodeoxynucleotide_Attenuates_Cancerous_Phenotype_in_Huh_7_Hepatocellular_Carcinoma_Cells">Application of XBP1s Decoy Oligodeoxynucleotide Attenuates Cancerous Phenotype in Huh-7 Hepatocellular Carcinoma Cells</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: The spliced form of X-box binding protein 1 (XBP1s) is a key transcription factor in t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: The spliced form of X-box binding protein 1 (XBP1s) is a key transcription factor in the unfolded protein response (UPR), an adaptive mechanism for cell survival. Many studies demonstrated the induced expression of XBP1s in various cancers, including hepatocellular carcinoma (HCC). Such upregulated expression is linked to an enhancement of cell proliferation, migration, and improvement of the survival rate. In this study, we aimed to assess the therapeutic potential of targeting XBP1s, by specific decoy oligodeoxynucleotide (ODN) and evaluated the cancerous phenotypes in Huh-7 cells. Materials and Methods: In this experimental study, we transfected Huh-7 cells with XBP1s decoy oligonucleotide (ODN). Subsequently, we assess some cellular features, including viability, migration capacity, proliferation potential, and apoptosis. Therefore, various techniques included wound healing test, BrdU, and annexin/PI assays. Additionally, the colony formation capacity was evaluated. The mRNA expression levels of BAX, BCL-2, c-MYC, CCND1, MMP-9, CDH1, and CD133 were quantified by the reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results: Transfection of Huh-7 cells by XBP1s decoy ODN led to significant down-regulation of c-Myc, CCND1, MMP-9, BCL-2 and CD133 and up-regulation of CDH1 and BAX transcriptional expressions in comparison with the vehicle group. Our results also demonstrated that transfection of XBP1s-decoy reduced HCC cell viability, proliferation, migration capacity as well as colonization ability in comparison with the vehicle group. Conclusion: These findings proposed the potential application of XBP1s-decoy ODN to reduce cancerous phenotypes such as cell proliferation, cell migration and apoptosis induction in the Huh-7 cell line. More experiments on other cell lines and primary cells could validate our results.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1a4e524033f69acbe2c47dfc0498f146" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377711,"asset_id":127680183,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377711/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680183"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680183"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680183; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680183]").text(description); $(".js-view-count[data-work-id=127680183]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680183; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680183']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1a4e524033f69acbe2c47dfc0498f146" } } $('.js-work-strip[data-work-id=127680183]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680183,"title":"Application of XBP1s Decoy Oligodeoxynucleotide Attenuates Cancerous Phenotype in Huh-7 Hepatocellular Carcinoma Cells","internal_url":"https://www.academia.edu/127680183/Application_of_XBP1s_Decoy_Oligodeoxynucleotide_Attenuates_Cancerous_Phenotype_in_Huh_7_Hepatocellular_Carcinoma_Cells","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377711,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377711/thumbnails/1.jpg","file_name":"Cell_J_26_505.pdf","download_url":"https://www.academia.edu/attachments/121377711/download_file","bulk_download_file_name":"Application_of_XBP1s_Decoy_Oligodeoxynuc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377711/Cell_J_26_505.pdf?1739686081=\u0026response-content-disposition=attachment%3B+filename%3DApplication_of_XBP1s_Decoy_Oligodeoxynuc.pdf\u0026Expires=1739691332\u0026Signature=OpSe46~jFRB2EsVIwQuS9EzNZms~Ved9B6Ir~ptbanGk7LYIvzGgHbY0Kq~ZTxVMJX4E3zu6HloqjnVoVXqBVFlkSZhFfXeUqgjY3OyZk0eNoFQcODqoKUO3lK9ap3TV33GQyGEAEsArEt84zllYW2keiCdOjmMJ6eJ4x2vkMTAHn~FXBTM2Gp-WGUl5YiEbRnVztGRwitVIB40BuBGEz-7DSoZiXdiynj4OeM6mAnBdLWpIH4vOGrcMgaWr1QNKxSwfL5NFk-DQWowrpNNNMHFT3RXI6mi9jildYLT0kt0iKxCrCKBxANrhbiKpDnrsrfvg1REcDEyS6immYH2~Gw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680072"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680072/Poncirin_Impact_on_Human_HER2_Breast_Cancer_Cells_Inhibiting_Proliferation_Metastasis_and_Tumor_Growth_in_Mice_Potentially_through_The_PI3K_AKT_Pathway"><img alt="Research paper thumbnail of Poncirin Impact on Human HER2 Breast Cancer Cells: Inhibiting Proliferation, Metastasis, and Tumor Growth in Mice Potentially through The PI3K/AKT Pathway" class="work-thumbnail" src="https://attachments.academia-assets.com/121377660/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680072/Poncirin_Impact_on_Human_HER2_Breast_Cancer_Cells_Inhibiting_Proliferation_Metastasis_and_Tumor_Growth_in_Mice_Potentially_through_The_PI3K_AKT_Pathway">Poncirin Impact on Human HER2 Breast Cancer Cells: Inhibiting Proliferation, Metastasis, and Tumor Growth in Mice Potentially through The PI3K/AKT Pathway</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Breast cancer is a prevalent and heterogeneous disease, with human epidermal growth fa...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Breast cancer is a prevalent and heterogeneous disease, with human epidermal growth factor receptor-2 (HER2) overexpression occurring in over 20% of cases. Poncirin, a biologically active flavonone derived from the immature dried fruits of Poncirus trifoliata, is a 7-O-neohesperidoside of isosakuranetin with a well-documented history in traditional Chinese medicine for its health-promoting properties. While the previous research hinted at its potential as an anticancer agent, its specific effects on HER2 overexpressing breast cancer cells remain largely unexplored. The aim of this study is to investigate the specific effects of Poncirin, on HER2 overexpressing breast cancer cells. Materials and Methods: In experimental study, we assessed cell proliferation using the CCK-8 assay and explored cell migration and invasion with transwell assays. Additionally, we evaluated colony formation ability and examined apoptosis through the acridine orange/ethidium bromide (AO/EB) and Annexin V-fluorescein isothiocyanate (FITC)/ propidium iodide (PI) staining methods. The study also delved into the molecular mechanisms involved by scrutinizing the phosphatidylinositol 3-kinase/serine-threonine protein kinase (PI3K/AKT) signaling pathway via Western blotting. Furthermore, the researchers conducted in vivo experiments using mouse models to corroborate the findings in a living organism. Results: Poncirin demonstrated a remarkable ability to selectively inhibit proliferation and metastasis of HER2 overexpressing breast cancer cells. Mechanistically, the compound seemed to exert its effects by modulating the PI3K/AKT signaling pathway, implying its central role in the observed anticancer effects. These findings were further substantiated by in vivo experiments, which consistently showed a reduction in tumor growth when poncirin was administered. Conclusion: This study underscores potential of poncirin as a potent agent for restraining the growth and metastasis of HER2 overexpressing breast cancer cells. The evidence suggests that poncirin efficacy may be attributed to its modulation possibly through PI3K/AKT pathway.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c08db02e4f05546d81598248daa7ff58" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377660,"asset_id":127680072,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377660/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680072"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680072"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680072; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680072]").text(description); $(".js-view-count[data-work-id=127680072]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680072; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680072']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c08db02e4f05546d81598248daa7ff58" } } $('.js-work-strip[data-work-id=127680072]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680072,"title":"Poncirin Impact on Human HER2 Breast Cancer Cells: Inhibiting Proliferation, Metastasis, and Tumor Growth in Mice Potentially through The PI3K/AKT Pathway","internal_url":"https://www.academia.edu/127680072/Poncirin_Impact_on_Human_HER2_Breast_Cancer_Cells_Inhibiting_Proliferation_Metastasis_and_Tumor_Growth_in_Mice_Potentially_through_The_PI3K_AKT_Pathway","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377660,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377660/thumbnails/1.jpg","file_name":"Cell_J_26_496.pdf","download_url":"https://www.academia.edu/attachments/121377660/download_file","bulk_download_file_name":"Poncirin_Impact_on_Human_HER2_Breast_Can.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377660/Cell_J_26_496.pdf?1739685814=\u0026response-content-disposition=attachment%3B+filename%3DPoncirin_Impact_on_Human_HER2_Breast_Can.pdf\u0026Expires=1739690877\u0026Signature=hBAnuniwGb2WISioOrHiF9hbDy~y8PqdBMQDJLJqfwmU9hHC1K4iM59RJA8EL3pKfEx4wBvYo9yKW7uUAT9wWtoYU~IvGM8QqXdr8nRYyMLui~uJrn4jnnv-TTV~PoaQdons94RcQpTLd24OgprkMAytr32DAqwm5VY8uPnSJor7OdBRH4XxeU69SYmtkdkcdvyO6rNxFd85YF95EpOVEGgut8F6B-tXIKQd7hBUVBk0izuJUnv5USWBgsDrpN18fFQzvnOospuXQ80jCSMB7BMJl4ytuE8exzGshX1iWAoO1ET-jbBV1XBjSo6DTXwJgdBuiHWlSPkVyT6D48DtoQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680030"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680030/Development_and_Characterization_of_A_Novel_SpyTagged_Modular_Nanobody_as_A_Detection_Platform_for_CD22_Positive_Cells"><img alt="Research paper thumbnail of Development and Characterization of A Novel SpyTagged Modular Nanobody as A Detection Platform for CD22-Positive Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/121377646/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680030/Development_and_Characterization_of_A_Novel_SpyTagged_Modular_Nanobody_as_A_Detection_Platform_for_CD22_Positive_Cells">Development and Characterization of A Novel SpyTagged Modular Nanobody as A Detection Platform for CD22-Positive Cells</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: CD22, as a surface protein of B cells, is used in the diagnosis and target-specific im...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: CD22, as a surface protein of B cells, is used in the diagnosis and target-specific immunotherapy of B-cell malignancies. SpyTag and SpyCatcher, on the other hand, are two covalently coupled proteins capable of developing a bi-or multi-specific modular protein. The aim of this study was to develop FITC-conjugated SpyCatcher-SpyTagged anti-CD22 Nanobody (FITC-SpyC-SpyT-CD22Nb) to recognize CD22 on the surface of malignant B cells. Materials and Methods: In this experimental study, the SpyTag-CD22Nb construct was subcloned into a pET22 vector and expressed in E. coli BL21 (DE3). After purification using His-tag affinity chromatography, the size of the eluted protein was confirmed on a Western blot. In addition, the SpyCatcher protein, subcloned into pET28, was expressed in E. coli BL21 (DE3), purified by His-tag affinity chromatography and subjected to FITC labeling. FITC-SpyCatcher and SpyTag-CD22Nb were coupled in a 1:1 molar ratio. The specific binding of the produced FITC-SpyC-SpyT-CD22Nb was tested using CD22-positive Raji and CD22-negative K562 cell lines and was evaluated by flow cytometry Results: SpyTag-CD22Nb and SpyCatcher were successfully expressed in E. coli BL21 (DE3). The 1:1 molar ratio of SpyTag-CD22Nb and FITC-SpyCatcher successfully formed FITC-SpyC-SpyT-CD22Nb at room temperature. The flow cytometry results showed that FITC-SpyC-SpyT-CD22Nb specifically binds to the CD22-positive Raji cells, while there is no binding to the CD22-negative K562 control cells. Conclusion: The novel FITC-SpyC-SpyT-CD22Nb produced in the present study is capable of detecting the surficial expression of CD22. According to our findings, FITC-SpyC-SpyT-CD22Nb is applicable for specific targeting of CD22 in a therapeutic manner, i.e., chimeric antigen receptor (CAR)-T cell therapy and antibody drug conjugates (ADCs).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3f98002817668a4f063e5bc8d89b3ae9" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377646,"asset_id":127680030,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377646/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680030"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680030"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680030; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680021"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680021/Therapeutic_Effects_of_Althaea_officinalis_L_and_Metformin_on_Estradiol_Induced_Polycystic_Ovary_Syndrome_in_Rats_Insights_into_The_PI3K_AKT_Pathway_Inflammation_and_Oxidative_Stress"><img alt="Research paper thumbnail of Therapeutic Effects of Althaea officinalis L. and Metformin on Estradiol-Induced Polycystic Ovary Syndrome in Rats: Insights into The PI3K/AKT Pathway, Inflammation, and Oxidative Stress" class="work-thumbnail" src="https://attachments.academia-assets.com/121377640/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680021/Therapeutic_Effects_of_Althaea_officinalis_L_and_Metformin_on_Estradiol_Induced_Polycystic_Ovary_Syndrome_in_Rats_Insights_into_The_PI3K_AKT_Pathway_Inflammation_and_Oxidative_Stress">Therapeutic Effects of Althaea officinalis L. and Metformin on Estradiol-Induced Polycystic Ovary Syndrome in Rats: Insights into The PI3K/AKT Pathway, Inflammation, and Oxidative Stress</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Polycystic ovary syndrome (PCOS) is one of the most important causes of infertility, i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Polycystic ovary syndrome (PCOS) is one of the most important causes of infertility, irregular menstrual cycles, and anovulation in women. The current study aimed to investigate the therapeutic effects of Althaea officinalis L. (A. officinale) extract on PCOS in rats. Materials and Methods: In this experimental study, 70 rats in 7 groups (n=10/group) were studied for three weeks as follows; healthy control (HC), patient (PCOS), metformin (PCOS+MET), A. officinale treatment (PCOS+250 and 500 mg/kg A. officinale) and synergistic (PCOS+MET+250 and 500 mg/kg A. officinale) groups. Luteinizing hormone (LH), folliclestimulating hormone (FSH), progesterone (P) and testosterone (T) levels as well as inflammatory cytokines were measured. Total antioxidant capacity and lipid peroxidation levels were analyzed in ovarian tissue. The expression of GLUT-4, AKT, PI3K, PTEN genes and Ki-67 was assessed by real-time polymerase chain reaction (PCR) and immunohistochemistry, respectively. Results: A. officinale alone and especially in combination with MET moderated inflammatory and antioxidant parameters compared to the PCOS and MET groups. A. officinale in synergistic groups increased the apoptosis of granulosa cells by activating the PI3K/AKT pathway, resulting in a rise in the number of Ki-67 positive cells (P<0.05). Furthermore, following A. officinale treatment the LH/FSH rate decreased and FSH and P increased (P<0.05). Also, A. officinale extract could effectively normalize estrus cycle duration close to the normal group. Conclusion: The extract of A. officinale, in combination with metformin, can enhance the hypothalamic-pituitaryovary (HPO) axis with synergistic anti-inflammatory and antioxidant effects. Additionally, the extract showed apoptotic effect on cystic granulosa cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e7217f6aee84f6dab65a1322d45e95a3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377640,"asset_id":127680021,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377640/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680021"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680021"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680021; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125098009"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125098009/High_Intensity_Interval_Training_and_Vitamin_D3_Supplementation_Decrease_CCL_5_and_CCR5_Expression_In_White_Adipose_Tissue_of_Diabetic_Rats_Fed_with_A_High_Fat_Diet_and_Streptozotocin"><img alt="Research paper thumbnail of High-Intensity Interval Training and Vitamin D3 Supplementation Decrease CCL-5 and CCR5 Expression In White Adipose Tissue of Diabetic Rats Fed with A High-Fat Diet and Streptozotocin" class="work-thumbnail" src="https://attachments.academia-assets.com/119202176/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125098009/High_Intensity_Interval_Training_and_Vitamin_D3_Supplementation_Decrease_CCL_5_and_CCR5_Expression_In_White_Adipose_Tissue_of_Diabetic_Rats_Fed_with_A_High_Fat_Diet_and_Streptozotocin">High-Intensity Interval Training and Vitamin D3 Supplementation Decrease CCL-5 and CCR5 Expression In White Adipose Tissue of Diabetic Rats Fed with A High-Fat Diet and Streptozotocin</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and CC motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM). Materials and Methods: The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks. Results: The levels of CCL-5 (P<0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P<0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P< 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P<0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05). Conclusion: The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ee39a96f203a356e5a289dd5466b4beb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119202176,"asset_id":125098009,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119202176/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125098009"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125098009"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125098009; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125098009]").text(description); $(".js-view-count[data-work-id=125098009]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125098009; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125098009']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ee39a96f203a356e5a289dd5466b4beb" } } $('.js-work-strip[data-work-id=125098009]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125098009,"title":"High-Intensity Interval Training and Vitamin D3 Supplementation Decrease CCL-5 and CCR5 Expression In White Adipose Tissue of Diabetic Rats Fed with A High-Fat Diet and Streptozotocin","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2027582.1560","abstract":"Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and CC motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM). Materials and Methods: The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks. Results: The levels of CCL-5 (P\u003c0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P\u003c0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P\u003c 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P\u003c0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05). Conclusion: The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7"},"translated_abstract":"Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and CC motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM). Materials and Methods: The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks. Results: The levels of CCL-5 (P\u003c0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P\u003c0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P\u003c 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P\u003c0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05). Conclusion: The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.","internal_url":"https://www.academia.edu/125098009/High_Intensity_Interval_Training_and_Vitamin_D3_Supplementation_Decrease_CCL_5_and_CCR5_Expression_In_White_Adipose_Tissue_of_Diabetic_Rats_Fed_with_A_High_Fat_Diet_and_Streptozotocin","translated_internal_url":"","created_at":"2024-10-28T00:35:57.834-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615489,"work_id":125098009,"tagging_user_id":126987384,"tagged_user_id":294848381,"co_author_invite_id":null,"email":"w***i@razi.ac.ir","affiliation":"Razi University of Kermanshah, Iran","display_order":1,"name":"Worya Tahmasebi","title":"High-Intensity Interval Training and Vitamin D3 Supplementation Decrease CCL-5 and CCR5 Expression In White Adipose Tissue of Diabetic Rats Fed with A High-Fat Diet and Streptozotocin"}],"downloadable_attachments":[{"id":119202176,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119202176/thumbnails/1.jpg","file_name":"Cell_J_26_465.pdf","download_url":"https://www.academia.edu/attachments/119202176/download_file","bulk_download_file_name":"High_Intensity_Interval_Training_and_Vit.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119202176/Cell_J_26_465-libre.pdf?1730101696=\u0026response-content-disposition=attachment%3B+filename%3DHigh_Intensity_Interval_Training_and_Vit.pdf\u0026Expires=1738763676\u0026Signature=KETei855gHtcs2HGgcrVMrQgT1sMt1wtKvBL4VUH-mC3kBi4cDHvtwGAsWu0KxdshInE0zJaFCgYT-1JVgnXRdpkqfAvPTSX37l8RBuRg3WOW~Uc-OmJAmWgSZC7EM08Ryuwh~B1EQKsmFOumjz7gnJNUvYy6iwthFAAIC23FjRgsZXv-f43RfoBhwyhqUmz8ccwKje6i368z7wyly9moGGr~lFHpAEnQOVU9HsPTEyE4hAYI5TAEALFKqtLwUYZ2XLwIM5ErTWBtm8-bjPoSaEz4iV1UvYEMvQFEtheDHYayk5OJjIiBqOhvWkpAAbvwoYI~EyI7RcbJ-GqBbV6jA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"High_Intensity_Interval_Training_and_Vitamin_D3_Supplementation_Decrease_CCL_5_and_CCR5_Expression_In_White_Adipose_Tissue_of_Diabetic_Rats_Fed_with_A_High_Fat_Diet_and_Streptozotocin","translated_slug":"","page_count":8,"language":"en","content_type":"Work","summary":"Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and CC motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM). Materials and Methods: The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks. Results: The levels of CCL-5 (P\u003c0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P\u003c0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P\u003c 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P\u003c0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05). Conclusion: The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119202176,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119202176/thumbnails/1.jpg","file_name":"Cell_J_26_465.pdf","download_url":"https://www.academia.edu/attachments/119202176/download_file","bulk_download_file_name":"High_Intensity_Interval_Training_and_Vit.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119202176/Cell_J_26_465-libre.pdf?1730101696=\u0026response-content-disposition=attachment%3B+filename%3DHigh_Intensity_Interval_Training_and_Vit.pdf\u0026Expires=1738763676\u0026Signature=KETei855gHtcs2HGgcrVMrQgT1sMt1wtKvBL4VUH-mC3kBi4cDHvtwGAsWu0KxdshInE0zJaFCgYT-1JVgnXRdpkqfAvPTSX37l8RBuRg3WOW~Uc-OmJAmWgSZC7EM08Ryuwh~B1EQKsmFOumjz7gnJNUvYy6iwthFAAIC23FjRgsZXv-f43RfoBhwyhqUmz8ccwKje6i368z7wyly9moGGr~lFHpAEnQOVU9HsPTEyE4hAYI5TAEALFKqtLwUYZ2XLwIM5ErTWBtm8-bjPoSaEz4iV1UvYEMvQFEtheDHYayk5OJjIiBqOhvWkpAAbvwoYI~EyI7RcbJ-GqBbV6jA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1383570,"name":"Vitamin D3","url":"https://www.academia.edu/Documents/in/Vitamin_D3"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097935"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097935/Mac_1_Alongside_Platelet_Monocyte_Aggregates_as_Potential_Markers_in_Acute_Coronary_Syndrome_A_Case_Control_Study"><img alt="Research paper thumbnail of Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study" class="work-thumbnail" src="https://attachments.academia-assets.com/119201977/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097935/Mac_1_Alongside_Platelet_Monocyte_Aggregates_as_Potential_Markers_in_Acute_Coronary_Syndrome_A_Case_Control_Study">Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://royaninstitute.academia.edu/CellJournalYakhteh">Cell Journal Yakhteh</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MohsenHamidpour1">Mohsen Hamidpour</a></span></div><div class="wp-workCard_item"><span>Volume 26, Issue 7 </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosc...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P<0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11b expression on monocytes were significantly elevated in patients (P<0.001) and were positively associated with PMA (β=19.09, P<0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06, P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals. (respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70). Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally, the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationship between these markers and ACS.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6755544423b4829d290af27b6c47753f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201977,"asset_id":125097935,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201977/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097935"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097935"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097935; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097935]").text(description); $(".js-view-count[data-work-id=125097935]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097935; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097935']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6755544423b4829d290af27b6c47753f" } } $('.js-work-strip[data-work-id=125097935]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097935,"title":"Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2024525.1527","abstract":"Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P\u003c0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11b expression on monocytes were significantly elevated in patients (P\u003c0.001) and were positively associated with PMA (β=19.09, P\u003c0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06, P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals. (respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70). Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally, the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationship between these markers and ACS.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7 "},"translated_abstract":"Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P\u003c0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11b expression on monocytes were significantly elevated in patients (P\u003c0.001) and were positively associated with PMA (β=19.09, P\u003c0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06, P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals. (respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70). Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally, the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationship between these markers and ACS.","internal_url":"https://www.academia.edu/125097935/Mac_1_Alongside_Platelet_Monocyte_Aggregates_as_Potential_Markers_in_Acute_Coronary_Syndrome_A_Case_Control_Study","translated_internal_url":"","created_at":"2024-10-28T00:33:35.072-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615480,"work_id":125097935,"tagging_user_id":126987384,"tagged_user_id":330410199,"co_author_invite_id":8272079,"email":"m***p@sbmu.ac.ir","display_order":1,"name":"Mohsen Hamidpour","title":"Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study"}],"downloadable_attachments":[{"id":119201977,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201977/thumbnails/1.jpg","file_name":"Cell_J_26_454.pdf","download_url":"https://www.academia.edu/attachments/119201977/download_file","bulk_download_file_name":"Mac_1_Alongside_Platelet_Monocyte_Aggreg.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201977/Cell_J_26_454-libre.pdf?1730101724=\u0026response-content-disposition=attachment%3B+filename%3DMac_1_Alongside_Platelet_Monocyte_Aggreg.pdf\u0026Expires=1738763676\u0026Signature=PopnZLREOsE2sBOxslo05GAND5LQ7eXZVsxG3N-YEkHP5Au7Uv0jpNwk2xkyaaLehMP6u6E8niPs6Q4u9K4BnozFneNrCYXr3kxRh2w~94wFGMSbkcc4ikeaONr44qmzxnJdfJmMhnsoDYQ7OWQ2LOZqPJu2pnOFRjWtkWtU7biJPUGMkGBd76RntEl6U3Bm4X-6NLJI~A821JcZRkYT4H2DTj1uClY9iNKf-HcaGw7cXX1n-UN5oG2PW-PkJifJVtaVVcObfSoowyYzwXqPUJjZK6-AynllT5LxEdk22Vm71b0xEVoRi3HD2v9a9Q7y3wqSBuJlAJ~fcob~7wE-iA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Mac_1_Alongside_Platelet_Monocyte_Aggregates_as_Potential_Markers_in_Acute_Coronary_Syndrome_A_Case_Control_Study","translated_slug":"","page_count":11,"language":"en","content_type":"Work","summary":"Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P\u003c0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11b expression on monocytes were significantly elevated in patients (P\u003c0.001) and were positively associated with PMA (β=19.09, P\u003c0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06, P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals. (respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70). Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally, the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationship between these markers and ACS.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201977,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201977/thumbnails/1.jpg","file_name":"Cell_J_26_454.pdf","download_url":"https://www.academia.edu/attachments/119201977/download_file","bulk_download_file_name":"Mac_1_Alongside_Platelet_Monocyte_Aggreg.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201977/Cell_J_26_454-libre.pdf?1730101724=\u0026response-content-disposition=attachment%3B+filename%3DMac_1_Alongside_Platelet_Monocyte_Aggreg.pdf\u0026Expires=1738763676\u0026Signature=PopnZLREOsE2sBOxslo05GAND5LQ7eXZVsxG3N-YEkHP5Au7Uv0jpNwk2xkyaaLehMP6u6E8niPs6Q4u9K4BnozFneNrCYXr3kxRh2w~94wFGMSbkcc4ikeaONr44qmzxnJdfJmMhnsoDYQ7OWQ2LOZqPJu2pnOFRjWtkWtU7biJPUGMkGBd76RntEl6U3Bm4X-6NLJI~A821JcZRkYT4H2DTj1uClY9iNKf-HcaGw7cXX1n-UN5oG2PW-PkJifJVtaVVcObfSoowyYzwXqPUJjZK6-AynllT5LxEdk22Vm71b0xEVoRi3HD2v9a9Q7y3wqSBuJlAJ~fcob~7wE-iA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097570"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097570/Local_Transplantation_of_Mesenchymal_Stromal_Cells_Is_Safe_and_Could_Alleviate_Kienb%C3%B6ck_Diseases_Complications_A_Clinical_Trial_Study"><img alt="Research paper thumbnail of Local Transplantation of Mesenchymal Stromal Cells Is Safe and Could Alleviate Kienböck Disease's Complications: A Clinical Trial Study" class="work-thumbnail" src="https://attachments.academia-assets.com/119201821/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097570/Local_Transplantation_of_Mesenchymal_Stromal_Cells_Is_Safe_and_Could_Alleviate_Kienb%C3%B6ck_Diseases_Complications_A_Clinical_Trial_Study">Local Transplantation of Mesenchymal Stromal Cells Is Safe and Could Alleviate Kienböck Disease's Complications: A Clinical Trial Study</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist movement. Various palliative methods have been proposed as therapeutic strategies for alleviating symptoms. Mesenchymal stromal cell transplantation has been suggested as an innovative and promising approach due to its potential for inducing regeneration and immunomodulation in the necrotic tissue. This study aims to evaluate the safety of autologous bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation after core decompression in Kienböck disease. Materials and Methods: In this phase I of an open-label clinical trial, three patients (one female and two males) with stage 2 Kienböck disease underwent autologous BM-MSCs transplantation following lunate core decompression. The patients were followed up for six months to assess safety as well as secondary clinical outcomes, including pain level, range of motion (ROM), and functional disability. Results: Safety of BM-MSCs injection following the core decompression was evaluated by recording post-treatment complications during the six-month follow-up. No adverse events (AEs) or severe AEs (SAEs) were reported, indicating that BM-MSCs injection after core decompression is a safe intervention. All patients showed a remarkable reduction in visual analog scale (VAS) scores and "Disabilities of the Arm, Shoulder, and Hand" (DASH) questionnaire scores, suggesting the therapeutic potential of this intervention. Moreover, an increase in the ROM indicated that BM-MSCs transplantation can improve wrist functionality. Additionally, radiographic assessments before and after cell infusion demonstrated a reduction in lunate sclerosis after six months of follow-up. Conclusion: The transplantation of autologous BM-MSCs following lunate core decompression seems to be a safe clinical intervention and may lead to pain relief in patients with Kienböck disease. Furthermore, this procedure may help prevent disease progression during the follow-up period (registration number: NCT02646007).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="677cf4d0d2bba32f088c4764f89a7cd0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201821,"asset_id":125097570,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201821/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097570"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097570"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097570; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097570]").text(description); $(".js-view-count[data-work-id=125097570]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097570; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097570']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "677cf4d0d2bba32f088c4764f89a7cd0" } } $('.js-work-strip[data-work-id=125097570]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097570,"title":"Local Transplantation of Mesenchymal Stromal Cells Is Safe and Could Alleviate Kienböck Disease's Complications: A Clinical Trial Study","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2028891.1572","abstract":"Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist movement. Various palliative methods have been proposed as therapeutic strategies for alleviating symptoms. Mesenchymal stromal cell transplantation has been suggested as an innovative and promising approach due to its potential for inducing regeneration and immunomodulation in the necrotic tissue. This study aims to evaluate the safety of autologous bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation after core decompression in Kienböck disease. Materials and Methods: In this phase I of an open-label clinical trial, three patients (one female and two males) with stage 2 Kienböck disease underwent autologous BM-MSCs transplantation following lunate core decompression. The patients were followed up for six months to assess safety as well as secondary clinical outcomes, including pain level, range of motion (ROM), and functional disability. Results: Safety of BM-MSCs injection following the core decompression was evaluated by recording post-treatment complications during the six-month follow-up. No adverse events (AEs) or severe AEs (SAEs) were reported, indicating that BM-MSCs injection after core decompression is a safe intervention. All patients showed a remarkable reduction in visual analog scale (VAS) scores and \"Disabilities of the Arm, Shoulder, and Hand\" (DASH) questionnaire scores, suggesting the therapeutic potential of this intervention. Moreover, an increase in the ROM indicated that BM-MSCs transplantation can improve wrist functionality. Additionally, radiographic assessments before and after cell infusion demonstrated a reduction in lunate sclerosis after six months of follow-up. Conclusion: The transplantation of autologous BM-MSCs following lunate core decompression seems to be a safe clinical intervention and may lead to pain relief in patients with Kienböck disease. Furthermore, this procedure may help prevent disease progression during the follow-up period (registration number: NCT02646007).","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7"},"translated_abstract":"Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist movement. Various palliative methods have been proposed as therapeutic strategies for alleviating symptoms. Mesenchymal stromal cell transplantation has been suggested as an innovative and promising approach due to its potential for inducing regeneration and immunomodulation in the necrotic tissue. This study aims to evaluate the safety of autologous bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation after core decompression in Kienböck disease. Materials and Methods: In this phase I of an open-label clinical trial, three patients (one female and two males) with stage 2 Kienböck disease underwent autologous BM-MSCs transplantation following lunate core decompression. The patients were followed up for six months to assess safety as well as secondary clinical outcomes, including pain level, range of motion (ROM), and functional disability. Results: Safety of BM-MSCs injection following the core decompression was evaluated by recording post-treatment complications during the six-month follow-up. No adverse events (AEs) or severe AEs (SAEs) were reported, indicating that BM-MSCs injection after core decompression is a safe intervention. All patients showed a remarkable reduction in visual analog scale (VAS) scores and \"Disabilities of the Arm, Shoulder, and Hand\" (DASH) questionnaire scores, suggesting the therapeutic potential of this intervention. Moreover, an increase in the ROM indicated that BM-MSCs transplantation can improve wrist functionality. Additionally, radiographic assessments before and after cell infusion demonstrated a reduction in lunate sclerosis after six months of follow-up. Conclusion: The transplantation of autologous BM-MSCs following lunate core decompression seems to be a safe clinical intervention and may lead to pain relief in patients with Kienböck disease. Furthermore, this procedure may help prevent disease progression during the follow-up period (registration number: NCT02646007).","internal_url":"https://www.academia.edu/125097570/Local_Transplantation_of_Mesenchymal_Stromal_Cells_Is_Safe_and_Could_Alleviate_Kienb%C3%B6ck_Diseases_Complications_A_Clinical_Trial_Study","translated_internal_url":"","created_at":"2024-10-28T00:21:45.805-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615439,"work_id":125097570,"tagging_user_id":126987384,"tagged_user_id":63914468,"co_author_invite_id":null,"email":"m***i@gmail.com","display_order":1,"name":"Marzieh ebrahimi","title":"Local Transplantation of Mesenchymal Stromal Cells Is Safe and Could Alleviate Kienböck Disease's Complications: A Clinical Trial Study"}],"downloadable_attachments":[{"id":119201821,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201821/thumbnails/1.jpg","file_name":"Cell_J_26_446.pdf","download_url":"https://www.academia.edu/attachments/119201821/download_file","bulk_download_file_name":"Local_Transplantation_of_Mesenchymal_Str.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201821/Cell_J_26_446-libre.pdf?1730101749=\u0026response-content-disposition=attachment%3B+filename%3DLocal_Transplantation_of_Mesenchymal_Str.pdf\u0026Expires=1738763676\u0026Signature=K312e4vmydI9Moa6~p6awxflsiKsuA0pCX2FLC7AnA2V~NCnnfgJOFb1k2mWrwvIgRgolCQEP5AJF2d9uwIQUpD9yMoLytrUEE8hUvF82GySKzOD6dRVA3mDGqJ3EDMg8n~ohP7USnTrFCus4Kw4UsvHZLiMGRsLd3E9zzGdYKFqmC8BzDgl4xWnG3ckUJddrNcTtIOazxYql1~Izp7fdEZ-iErtooccHcZGPfU8ngGT-e4mO9KJ2Ivy8F023hLCuz7M8~IiOtXVuy~I8OTpdC2AxgS88PpQvQBpNNGCLy~p0o2gtVxfHuUh65MVDge1YCbCysZ~O5F0Xe8HVM3ZFA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Local_Transplantation_of_Mesenchymal_Stromal_Cells_Is_Safe_and_Could_Alleviate_Kienböck_Diseases_Complications_A_Clinical_Trial_Study","translated_slug":"","page_count":8,"language":"en","content_type":"Work","summary":"Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist movement. Various palliative methods have been proposed as therapeutic strategies for alleviating symptoms. Mesenchymal stromal cell transplantation has been suggested as an innovative and promising approach due to its potential for inducing regeneration and immunomodulation in the necrotic tissue. This study aims to evaluate the safety of autologous bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation after core decompression in Kienböck disease. Materials and Methods: In this phase I of an open-label clinical trial, three patients (one female and two males) with stage 2 Kienböck disease underwent autologous BM-MSCs transplantation following lunate core decompression. The patients were followed up for six months to assess safety as well as secondary clinical outcomes, including pain level, range of motion (ROM), and functional disability. Results: Safety of BM-MSCs injection following the core decompression was evaluated by recording post-treatment complications during the six-month follow-up. No adverse events (AEs) or severe AEs (SAEs) were reported, indicating that BM-MSCs injection after core decompression is a safe intervention. All patients showed a remarkable reduction in visual analog scale (VAS) scores and \"Disabilities of the Arm, Shoulder, and Hand\" (DASH) questionnaire scores, suggesting the therapeutic potential of this intervention. Moreover, an increase in the ROM indicated that BM-MSCs transplantation can improve wrist functionality. Additionally, radiographic assessments before and after cell infusion demonstrated a reduction in lunate sclerosis after six months of follow-up. Conclusion: The transplantation of autologous BM-MSCs following lunate core decompression seems to be a safe clinical intervention and may lead to pain relief in patients with Kienböck disease. Furthermore, this procedure may help prevent disease progression during the follow-up period (registration number: NCT02646007).","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201821,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201821/thumbnails/1.jpg","file_name":"Cell_J_26_446.pdf","download_url":"https://www.academia.edu/attachments/119201821/download_file","bulk_download_file_name":"Local_Transplantation_of_Mesenchymal_Str.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201821/Cell_J_26_446-libre.pdf?1730101749=\u0026response-content-disposition=attachment%3B+filename%3DLocal_Transplantation_of_Mesenchymal_Str.pdf\u0026Expires=1738763676\u0026Signature=K312e4vmydI9Moa6~p6awxflsiKsuA0pCX2FLC7AnA2V~NCnnfgJOFb1k2mWrwvIgRgolCQEP5AJF2d9uwIQUpD9yMoLytrUEE8hUvF82GySKzOD6dRVA3mDGqJ3EDMg8n~ohP7USnTrFCus4Kw4UsvHZLiMGRsLd3E9zzGdYKFqmC8BzDgl4xWnG3ckUJddrNcTtIOazxYql1~Izp7fdEZ-iErtooccHcZGPfU8ngGT-e4mO9KJ2Ivy8F023hLCuz7M8~IiOtXVuy~I8OTpdC2AxgS88PpQvQBpNNGCLy~p0o2gtVxfHuUh65MVDge1YCbCysZ~O5F0Xe8HVM3ZFA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3176577,"name":"Avascular Necrosis","url":"https://www.academia.edu/Documents/in/Avascular_Necrosis"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097338"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097338/Potential_Advantages_of_Idarubicin_Loaded_Trastuzumab_Coated_Liposomes_for_Combating_Head_and_Neck_Squamous_Cancer_Cells"><img alt="Research paper thumbnail of Potential Advantages of Idarubicin-Loaded Trastuzumab-Coated Liposomes for Combating Head and Neck Squamous Cancer Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/119201626/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097338/Potential_Advantages_of_Idarubicin_Loaded_Trastuzumab_Coated_Liposomes_for_Combating_Head_and_Neck_Squamous_Cancer_Cells">Potential Advantages of Idarubicin-Loaded Trastuzumab-Coated Liposomes for Combating Head and Neck Squamous Cancer Cells</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7 </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Head and neck squamous cell carcinoma (HNSCC) with a high mortality rate is among the ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Head and neck squamous cell carcinoma (HNSCC) with a high mortality rate is among the most common types of cancer in the world. Human epidermal growth factor receptor 2 (HER2) is expressed higher than normal level in the most HNSCC tumors, making them resistant to chemotherapy and radiotherapy. Therefore, HER2 has been introduced as a suitable target for anticancer drugs. The aim of this study is to examine the efficacy of a treatment protocol involving targeted delivery of idarubicin encapsulated in trastuzumab-decorated liposomes to HNSCC cells. Materials and Methods: In the current experimental study, efficacies of idarubicin, prepared liposomal idarubicin, and constructed immunoliposomal idarubicin (trastuzumab-decorated) were investigated in killing HN5 cells, a HER2overexpressing HNSCC-originating cell line. Liposomal content of idarubicin and trastuzumab were qualified by UV-Visible spectroscopy and preparations were characterized for shape and size by atomic force microscopy (AFM) and dynamic light scattering (DLS). To clarify role of the missing parts of the available crystal structure (PDB ID: 1n8z) within trastuzumab-HER2 interactions, we used a 40 ns molecular dynamic simulation approach. Results: Based on the obtained results, liposomal idarubicin showed higher toxicity of the encapsulated drug on HN5 cells compared to the traditional free drug formulations. The immunoliposomal form of idarubicin was more effective than the liposomal formulation, in killing of HN5 cells. In addition, simulation of interactions between trastuzumab and HER2 revealed that the missing parts (in the crystal structure) of HER2 have critical interaction with trastuzumab, through salt-bridges and hydrogen bonds. Conclusion: It seems that the prepared immunoliposomes could attach more efficiently to HER2 overexpressing cells, which consequently leads to increasing cellular uptake of idarubicin through a receptor-mediated endocytosis mechanism. Moreover, simulation of the interaction between HER2 and trastuzumab suggested considerable possibilities for increasing trastuzumab affinity to HER2.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d4d6693bca59a9a91bcda91e44221f90" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201626,"asset_id":125097338,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201626/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097338"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097338"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097338; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097338]").text(description); $(".js-view-count[data-work-id=125097338]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097338; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097338']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d4d6693bca59a9a91bcda91e44221f90" } } $('.js-work-strip[data-work-id=125097338]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097338,"title":"Potential Advantages of Idarubicin-Loaded Trastuzumab-Coated Liposomes for Combating Head and Neck Squamous Cancer Cells","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2019704.1480","abstract":"Objective: Head and neck squamous cell carcinoma (HNSCC) with a high mortality rate is among the most common types of cancer in the world. Human epidermal growth factor receptor 2 (HER2) is expressed higher than normal level in the most HNSCC tumors, making them resistant to chemotherapy and radiotherapy. Therefore, HER2 has been introduced as a suitable target for anticancer drugs. The aim of this study is to examine the efficacy of a treatment protocol involving targeted delivery of idarubicin encapsulated in trastuzumab-decorated liposomes to HNSCC cells. Materials and Methods: In the current experimental study, efficacies of idarubicin, prepared liposomal idarubicin, and constructed immunoliposomal idarubicin (trastuzumab-decorated) were investigated in killing HN5 cells, a HER2overexpressing HNSCC-originating cell line. Liposomal content of idarubicin and trastuzumab were qualified by UV-Visible spectroscopy and preparations were characterized for shape and size by atomic force microscopy (AFM) and dynamic light scattering (DLS). 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In addition, simulation of interactions between trastuzumab and HER2 revealed that the missing parts (in the crystal structure) of HER2 have critical interaction with trastuzumab, through salt-bridges and hydrogen bonds. Conclusion: It seems that the prepared immunoliposomes could attach more efficiently to HER2 overexpressing cells, which consequently leads to increasing cellular uptake of idarubicin through a receptor-mediated endocytosis mechanism. 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Human epidermal growth factor receptor 2 (HER2) is expressed higher than normal level in the most HNSCC tumors, making them resistant to chemotherapy and radiotherapy. Therefore, HER2 has been introduced as a suitable target for anticancer drugs. The aim of this study is to examine the efficacy of a treatment protocol involving targeted delivery of idarubicin encapsulated in trastuzumab-decorated liposomes to HNSCC cells. Materials and Methods: In the current experimental study, efficacies of idarubicin, prepared liposomal idarubicin, and constructed immunoliposomal idarubicin (trastuzumab-decorated) were investigated in killing HN5 cells, a HER2overexpressing HNSCC-originating cell line. Liposomal content of idarubicin and trastuzumab were qualified by UV-Visible spectroscopy and preparations were characterized for shape and size by atomic force microscopy (AFM) and dynamic light scattering (DLS). To clarify role of the missing parts of the available crystal structure (PDB ID: 1n8z) within trastuzumab-HER2 interactions, we used a 40 ns molecular dynamic simulation approach. Results: Based on the obtained results, liposomal idarubicin showed higher toxicity of the encapsulated drug on HN5 cells compared to the traditional free drug formulations. The immunoliposomal form of idarubicin was more effective than the liposomal formulation, in killing of HN5 cells. In addition, simulation of interactions between trastuzumab and HER2 revealed that the missing parts (in the crystal structure) of HER2 have critical interaction with trastuzumab, through salt-bridges and hydrogen bonds. Conclusion: It seems that the prepared immunoliposomes could attach more efficiently to HER2 overexpressing cells, which consequently leads to increasing cellular uptake of idarubicin through a receptor-mediated endocytosis mechanism. Moreover, simulation of the interaction between HER2 and trastuzumab suggested considerable possibilities for increasing trastuzumab affinity to HER2.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201626,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201626/thumbnails/1.jpg","file_name":"Cell_J_26_436.pdf","download_url":"https://www.academia.edu/attachments/119201626/download_file","bulk_download_file_name":"Potential_Advantages_of_Idarubicin_Loade.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201626/Cell_J_26_436-libre.pdf?1730101791=\u0026response-content-disposition=attachment%3B+filename%3DPotential_Advantages_of_Idarubicin_Loade.pdf\u0026Expires=1738763676\u0026Signature=LT9-P2~OPy4INV9xPuhTLBvMtUWG0L4hMQ5PX4kSNeazWXibOsymrCZxYV1ufQ5-rK~JltWke~P1y8nIFLawdAqDYuItT4uwp5dANBeP1T8fYxa5gsVYmqeT-hhIh7onzp~2S17nejqK8BvvJD9vzpaM1hQ-Q-3kMg5zDJTNfDpxiZxoravEycyt8MhxwzEdO6DNND7KRKayMP6fDFZ-8PWrg1F1lB2RZQrIgH56g7UDZhkbgI3R5NmreV6s4P7J853vg92hrALe9czk9ZmWL16SwwnPuL93eNruDUWeueGlpdg~td66VMsek3TAZPrEEtj0mlR-N3vC1s1etA8a9A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":6021,"name":"Cancer","url":"https://www.academia.edu/Documents/in/Cancer"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097144"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097144/Production_of_Cartilaginous_Organoids_Potential_Opportunities_and_Challenges_A_Review_Article"><img alt="Research paper thumbnail of Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article" class="work-thumbnail" src="https://attachments.academia-assets.com/119201498/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097144/Production_of_Cartilaginous_Organoids_Potential_Opportunities_and_Challenges_A_Review_Article">Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7 </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, and dysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment. Two dimensional (2D) and three-dimensional (3D) cell cultures can help to study growth modeling and physiological characteristics of the human body. Among the problems that 2D and single-layer cultures have the lack of proper and accurate tissue modeling and the lack of tissue complications similar is to the original tissue. With organoid models, cellular and tissue structural studies and functional and physiological studies of tissues have been revolutionized and more accurate. Organoids are useful for studying repair and drug efficacy. Physiological and pathological investigations by combining in vitro and in vivo methods have become more effective today. The purpose of this study is to investigate the factors involved in the formation of cartilage organoids so that we can introduce the best method of organoid production for the healing of cartilage damage by using cell types and organoid model.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bf7cac054c7a1dbb0ae19d16a2213693" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201498,"asset_id":125097144,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201498/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097144"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097144"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097144; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097144]").text(description); $(".js-view-count[data-work-id=125097144]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097144; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097144']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bf7cac054c7a1dbb0ae19d16a2213693" } } $('.js-work-strip[data-work-id=125097144]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097144,"title":"Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2023118.1510","abstract":"Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, and dysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment. Two dimensional (2D) and three-dimensional (3D) cell cultures can help to study growth modeling and physiological characteristics of the human body. Among the problems that 2D and single-layer cultures have the lack of proper and accurate tissue modeling and the lack of tissue complications similar is to the original tissue. With organoid models, cellular and tissue structural studies and functional and physiological studies of tissues have been revolutionized and more accurate. Organoids are useful for studying repair and drug efficacy. Physiological and pathological investigations by combining in vitro and in vivo methods have become more effective today. The purpose of this study is to investigate the factors involved in the formation of cartilage organoids so that we can introduce the best method of organoid production for the healing of cartilage damage by using cell types and organoid model.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7 "},"translated_abstract":"Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, and dysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment. Two dimensional (2D) and three-dimensional (3D) cell cultures can help to study growth modeling and physiological characteristics of the human body. Among the problems that 2D and single-layer cultures have the lack of proper and accurate tissue modeling and the lack of tissue complications similar is to the original tissue. With organoid models, cellular and tissue structural studies and functional and physiological studies of tissues have been revolutionized and more accurate. Organoids are useful for studying repair and drug efficacy. Physiological and pathological investigations by combining in vitro and in vivo methods have become more effective today. The purpose of this study is to investigate the factors involved in the formation of cartilage organoids so that we can introduce the best method of organoid production for the healing of cartilage damage by using cell types and organoid model.","internal_url":"https://www.academia.edu/125097144/Production_of_Cartilaginous_Organoids_Potential_Opportunities_and_Challenges_A_Review_Article","translated_internal_url":"","created_at":"2024-10-28T00:09:51.349-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615386,"work_id":125097144,"tagging_user_id":126987384,"tagged_user_id":null,"co_author_invite_id":8272068,"email":"a***i@sums.ac.ir","display_order":1,"name":"Ali Rajabi","title":"Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article"}],"downloadable_attachments":[{"id":119201498,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201498/thumbnails/1.jpg","file_name":"Cell_J_26_427.pdf","download_url":"https://www.academia.edu/attachments/119201498/download_file","bulk_download_file_name":"Production_of_Cartilaginous_Organoids_Po.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201498/Cell_J_26_427-libre.pdf?1730101804=\u0026response-content-disposition=attachment%3B+filename%3DProduction_of_Cartilaginous_Organoids_Po.pdf\u0026Expires=1738763676\u0026Signature=LYDq9hx1I6qT~T2Hp-ysmq6kko7LaGw8cpDnZ6oAEt-iLnUvz9jDvZjXY87T21HSyIk-EG4OdUJtDv80CiaNOni0mvGNz2e0Gk6NthVMWyePAw1q9~1SEuPTo1URNzboV~E61dQSwOD~Ew7Fi9M-WGG5-m5-3fvZmSAbg51t6GvYtujRKAy2Run-w0skH057NLOeeNOaHEzXotqwYYz8Ys2Cw4MbvIqS6pV3Mv4pZqBSa60xyIdfUszMO95v9qkdf2avUlUhlinQ-9F9mXyzhx8YzWtGxC6qn8aUDKIq4yeuSowrX6qoe5Ugwct~M4Z9SgEM2X3yWgr0K0GV2pE2LA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Production_of_Cartilaginous_Organoids_Potential_Opportunities_and_Challenges_A_Review_Article","translated_slug":"","page_count":9,"language":"en","content_type":"Work","summary":"Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, and dysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment. Two dimensional (2D) and three-dimensional (3D) cell cultures can help to study growth modeling and physiological characteristics of the human body. Among the problems that 2D and single-layer cultures have the lack of proper and accurate tissue modeling and the lack of tissue complications similar is to the original tissue. With organoid models, cellular and tissue structural studies and functional and physiological studies of tissues have been revolutionized and more accurate. Organoids are useful for studying repair and drug efficacy. Physiological and pathological investigations by combining in vitro and in vivo methods have become more effective today. The purpose of this study is to investigate the factors involved in the formation of cartilage organoids so that we can introduce the best method of organoid production for the healing of cartilage damage by using cell types and organoid model.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201498,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201498/thumbnails/1.jpg","file_name":"Cell_J_26_427.pdf","download_url":"https://www.academia.edu/attachments/119201498/download_file","bulk_download_file_name":"Production_of_Cartilaginous_Organoids_Po.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201498/Cell_J_26_427-libre.pdf?1730101804=\u0026response-content-disposition=attachment%3B+filename%3DProduction_of_Cartilaginous_Organoids_Po.pdf\u0026Expires=1738763676\u0026Signature=LYDq9hx1I6qT~T2Hp-ysmq6kko7LaGw8cpDnZ6oAEt-iLnUvz9jDvZjXY87T21HSyIk-EG4OdUJtDv80CiaNOni0mvGNz2e0Gk6NthVMWyePAw1q9~1SEuPTo1URNzboV~E61dQSwOD~Ew7Fi9M-WGG5-m5-3fvZmSAbg51t6GvYtujRKAy2Run-w0skH057NLOeeNOaHEzXotqwYYz8Ys2Cw4MbvIqS6pV3Mv4pZqBSa60xyIdfUszMO95v9qkdf2avUlUhlinQ-9F9mXyzhx8YzWtGxC6qn8aUDKIq4yeuSowrX6qoe5Ugwct~M4Z9SgEM2X3yWgr0K0GV2pE2LA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":13827,"name":"Cell Biology","url":"https://www.academia.edu/Documents/in/Cell_Biology"},{"id":78188,"name":"Cartilage","url":"https://www.academia.edu/Documents/in/Cartilage"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097065"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097065/A_Comparative_Analysis_of_Computational_Strategies_in_Multi_Epitope_Vaccine_Design_Against_Human_Papillomavirus_and_Cervical_Cancer"><img alt="Research paper thumbnail of A Comparative Analysis of Computational Strategies in Multi-Epitope Vaccine Design Against Human Papillomavirus and Cervical Cancer" class="work-thumbnail" src="https://attachments.academia-assets.com/119201419/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097065/A_Comparative_Analysis_of_Computational_Strategies_in_Multi_Epitope_Vaccine_Design_Against_Human_Papillomavirus_and_Cervical_Cancer">A Comparative Analysis of Computational Strategies in Multi-Epitope Vaccine Design Against Human Papillomavirus and Cervical Cancer</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other m...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other malignancies, there is a need for innovative approaches to preventing this infection. It has been shown that immunoinformatics is an important strategy in computational vaccinology. It is used to design new multi-epitope vaccines against different types of HPV and subsequent cervical cancer. This paper reviews the scope of the entire computational pipeline of HPV vaccine design, starting from data analysis at the genomic and proteomic levels and continuing to epitope predictions of the innate and adaptive immune systems. The search strategy was based on investigating original articles published in "Google Scholar" and "PubMed" from 2015 to 2023-2024. The terms "Immunoinformatics", "Bioinformatics", "Human papillomavirus (HPV)", "Vaccine design", "In silico vaccine design", "Multi-epitope vaccine design", "Vaccinology" and "HPV vaccine" were used to for this purpose. We discussed various essential tools involved in the computational design of the vaccine process, e.g., sequence analysis, epitope prediction, conservancy analysis, tertiary structure modeling, refinement, molecular docking, molecular dynamics (MD) simulation, and in silico cloning. This review article describes immunoinformatics methods that facilitate the design of a multi-epitope vaccine against HPV. However, this pipeline can also be used to design novel chimeric vaccines for other pathogens.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1a9fae34cccf3bd3471946fcd911c114" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201419,"asset_id":125097065,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201419/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097065"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097065"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097065; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097065]").text(description); $(".js-view-count[data-work-id=125097065]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097065; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097065']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1a9fae34cccf3bd3471946fcd911c114" } } $('.js-work-strip[data-work-id=125097065]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097065,"title":"A Comparative Analysis of Computational Strategies in Multi-Epitope Vaccine Design Against Human Papillomavirus and Cervical Cancer","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2028622.1568","abstract":"Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other malignancies, there is a need for innovative approaches to preventing this infection. It has been shown that immunoinformatics is an important strategy in computational vaccinology. It is used to design new multi-epitope vaccines against different types of HPV and subsequent cervical cancer. This paper reviews the scope of the entire computational pipeline of HPV vaccine design, starting from data analysis at the genomic and proteomic levels and continuing to epitope predictions of the innate and adaptive immune systems. The search strategy was based on investigating original articles published in \"Google Scholar\" and \"PubMed\" from 2015 to 2023-2024. The terms \"Immunoinformatics\", \"Bioinformatics\", \"Human papillomavirus (HPV)\", \"Vaccine design\", \"In silico vaccine design\", \"Multi-epitope vaccine design\", \"Vaccinology\" and \"HPV vaccine\" were used to for this purpose. We discussed various essential tools involved in the computational design of the vaccine process, e.g., sequence analysis, epitope prediction, conservancy analysis, tertiary structure modeling, refinement, molecular docking, molecular dynamics (MD) simulation, and in silico cloning. This review article describes immunoinformatics methods that facilitate the design of a multi-epitope vaccine against HPV. However, this pipeline can also be used to design novel chimeric vaccines for other pathogens.","ai_title_tag":"Immunoinformatics for HPV Multi-Epitope Vaccines","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7"},"translated_abstract":"Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other malignancies, there is a need for innovative approaches to preventing this infection. It has been shown that immunoinformatics is an important strategy in computational vaccinology. It is used to design new multi-epitope vaccines against different types of HPV and subsequent cervical cancer. This paper reviews the scope of the entire computational pipeline of HPV vaccine design, starting from data analysis at the genomic and proteomic levels and continuing to epitope predictions of the innate and adaptive immune systems. The search strategy was based on investigating original articles published in \"Google Scholar\" and \"PubMed\" from 2015 to 2023-2024. The terms \"Immunoinformatics\", \"Bioinformatics\", \"Human papillomavirus (HPV)\", \"Vaccine design\", \"In silico vaccine design\", \"Multi-epitope vaccine design\", \"Vaccinology\" and \"HPV vaccine\" were used to for this purpose. We discussed various essential tools involved in the computational design of the vaccine process, e.g., sequence analysis, epitope prediction, conservancy analysis, tertiary structure modeling, refinement, molecular docking, molecular dynamics (MD) simulation, and in silico cloning. This review article describes immunoinformatics methods that facilitate the design of a multi-epitope vaccine against HPV. However, this pipeline can also be used to design novel chimeric vaccines for other pathogens.","internal_url":"https://www.academia.edu/125097065/A_Comparative_Analysis_of_Computational_Strategies_in_Multi_Epitope_Vaccine_Design_Against_Human_Papillomavirus_and_Cervical_Cancer","translated_internal_url":"","created_at":"2024-10-28T00:06:16.236-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615377,"work_id":125097065,"tagging_user_id":126987384,"tagged_user_id":null,"co_author_invite_id":8272066,"email":"e***h@bmsu.ac.ir","display_order":1,"name":"Hadi Esmaeili","title":"A Comparative Analysis of Computational Strategies in Multi-Epitope Vaccine Design Against Human Papillomavirus and Cervical Cancer"}],"downloadable_attachments":[{"id":119201419,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201419/thumbnails/1.jpg","file_name":"Cell_J_26_403.pdf","download_url":"https://www.academia.edu/attachments/119201419/download_file","bulk_download_file_name":"A_Comparative_Analysis_of_Computational.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201419/Cell_J_26_403-libre.pdf?1730104274=\u0026response-content-disposition=attachment%3B+filename%3DA_Comparative_Analysis_of_Computational.pdf\u0026Expires=1738725410\u0026Signature=cD2YafcVymRm3pDKncg2qtsEEDDuDMZUZZ1bnrjL-DtzfOhRdeF-rg-9dZqKYYvlyfgkGEus2CeDpOUxjE4~3VbVWKxivEqfbtH1A7UxvOVcGif5YZLO1GfRHcGhBgPL6jiYUjohzLIWhDOsRlqdh59FVlyO66XbJ8S2EipWTz0vQsEWOJ3lJsAOkdO4nvL0~T7f6MDSsPMlErP~XWhGLoS~kRbpoXNAaN2vnt4-Nzq057~vriG0zRRIuyzKL7Re81~w0abCtpIDVfLN3Mm7bGhTd1tt3cx8O8m1PifUJjphxI4X1E5lnShav0zUEpVniW0yI7pq1j~2xVm9dvF5yg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_Comparative_Analysis_of_Computational_Strategies_in_Multi_Epitope_Vaccine_Design_Against_Human_Papillomavirus_and_Cervical_Cancer","translated_slug":"","page_count":24,"language":"en","content_type":"Work","summary":"Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other malignancies, there is a need for innovative approaches to preventing this infection. It has been shown that immunoinformatics is an important strategy in computational vaccinology. It is used to design new multi-epitope vaccines against different types of HPV and subsequent cervical cancer. This paper reviews the scope of the entire computational pipeline of HPV vaccine design, starting from data analysis at the genomic and proteomic levels and continuing to epitope predictions of the innate and adaptive immune systems. The search strategy was based on investigating original articles published in \"Google Scholar\" and \"PubMed\" from 2015 to 2023-2024. The terms \"Immunoinformatics\", \"Bioinformatics\", \"Human papillomavirus (HPV)\", \"Vaccine design\", \"In silico vaccine design\", \"Multi-epitope vaccine design\", \"Vaccinology\" and \"HPV vaccine\" were used to for this purpose. We discussed various essential tools involved in the computational design of the vaccine process, e.g., sequence analysis, epitope prediction, conservancy analysis, tertiary structure modeling, refinement, molecular docking, molecular dynamics (MD) simulation, and in silico cloning. This review article describes immunoinformatics methods that facilitate the design of a multi-epitope vaccine against HPV. However, this pipeline can also be used to design novel chimeric vaccines for other pathogens.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201419,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201419/thumbnails/1.jpg","file_name":"Cell_J_26_403.pdf","download_url":"https://www.academia.edu/attachments/119201419/download_file","bulk_download_file_name":"A_Comparative_Analysis_of_Computational.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201419/Cell_J_26_403-libre.pdf?1730104274=\u0026response-content-disposition=attachment%3B+filename%3DA_Comparative_Analysis_of_Computational.pdf\u0026Expires=1738725410\u0026Signature=cD2YafcVymRm3pDKncg2qtsEEDDuDMZUZZ1bnrjL-DtzfOhRdeF-rg-9dZqKYYvlyfgkGEus2CeDpOUxjE4~3VbVWKxivEqfbtH1A7UxvOVcGif5YZLO1GfRHcGhBgPL6jiYUjohzLIWhDOsRlqdh59FVlyO66XbJ8S2EipWTz0vQsEWOJ3lJsAOkdO4nvL0~T7f6MDSsPMlErP~XWhGLoS~kRbpoXNAaN2vnt4-Nzq057~vriG0zRRIuyzKL7Re81~w0abCtpIDVfLN3Mm7bGhTd1tt3cx8O8m1PifUJjphxI4X1E5lnShav0zUEpVniW0yI7pq1j~2xVm9dvF5yg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":50351,"name":"HUMAN PAPILLOMAVIRUS","url":"https://www.academia.edu/Documents/in/HUMAN_PAPILLOMAVIRUS"},{"id":173523,"name":"HPV","url":"https://www.academia.edu/Documents/in/HPV"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123077776"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123077776/Conditioned_Medium_Protects_Dopaminergic_Neurons_in_Parkinsonian_Rats"><img alt="Research paper thumbnail of Conditioned Medium Protects Dopaminergic Neurons in Parkinsonian Rats" class="work-thumbnail" src="https://attachments.academia-assets.com/117598261/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123077776/Conditioned_Medium_Protects_Dopaminergic_Neurons_in_Parkinsonian_Rats">Conditioned Medium Protects Dopaminergic Neurons in Parkinsonian Rats</a></div><div class="wp-workCard_item"><span>Cell journal</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditio...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditioned medium (CM), which protect neurons by its antioxidative and trophic effects. This research assesses the neuroprotective effect of ASCCM on neurotrophins genes expressions and tyrosine hydroxylase positive (TH) cell density in male Wistar rats lesioned by 6-hydroxydopamine (6-OHDA). In this experimental study, the groups consisted of lesioned and sham rats with unilateral injections of 20 μg of 6-OHDA neurotoxin and phosphate buffered saline (PBS) into the striatum, respectively. Another groups received intravenous injections of 3×106 cells (ASCs group), 500 μl of CM (ASC-CM group) or medium [α-minimal essential medium (α-MEM) group)]. All rats underwent evaluations with the rotarod and apomorphine-induced rotation tests at 2, 4, 6, and 8 weeks post-injection. At 8 weeks we sacrificed some of the animals for real-time polymerase chain reaction (PCR) analysis, and evaluation of TH cell...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b267615cd882d29a773989703c5674d2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117598261,"asset_id":123077776,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117598261/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123077776"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123077776"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123077776; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123077776]").text(description); $(".js-view-count[data-work-id=123077776]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123077776; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123077776']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b267615cd882d29a773989703c5674d2" } } $('.js-work-strip[data-work-id=123077776]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123077776,"title":"Conditioned Medium Protects Dopaminergic Neurons in Parkinsonian Rats","translated_title":"","metadata":{"abstract":"Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditioned medium (CM), which protect neurons by its antioxidative and trophic effects. This research assesses the neuroprotective effect of ASCCM on neurotrophins genes expressions and tyrosine hydroxylase positive (TH) cell density in male Wistar rats lesioned by 6-hydroxydopamine (6-OHDA). In this experimental study, the groups consisted of lesioned and sham rats with unilateral injections of 20 μg of 6-OHDA neurotoxin and phosphate buffered saline (PBS) into the striatum, respectively. Another groups received intravenous injections of 3×106 cells (ASCs group), 500 μl of CM (ASC-CM group) or medium [α-minimal essential medium (α-MEM) group)]. All rats underwent evaluations with the rotarod and apomorphine-induced rotation tests at 2, 4, 6, and 8 weeks post-injection. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123077647"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123077647/Exploring_Advances_in_Reproduction_and_Stem_Cell_Biology_Highlights_from_The_24th_and_19th_International_Congresses_in_Iran"><img alt="Research paper thumbnail of Exploring Advances in Reproduction and Stem Cell Biology: Highlights from The 24th and 19th International Congresses in Iran" class="work-thumbnail" src="https://attachments.academia-assets.com/117598128/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123077647/Exploring_Advances_in_Reproduction_and_Stem_Cell_Biology_Highlights_from_The_24th_and_19th_International_Congresses_in_Iran">Exploring Advances in Reproduction and Stem Cell Biology: Highlights from The 24th and 19th International Congresses in Iran</a></div><div class="wp-workCard_item"><span>Vol 26, No 6, June </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The 24th and 19th International Congresses on Reproduction and Stem Cell Biology in the Islamic R...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The 24th and 19th International Congresses on Reproduction and Stem Cell Biology in the Islamic Republic of Iran brought<br />together experts and researchers worldwide to explore the latest advancements in these fields. Different topics were<br />discussed, including such as reproductive health, infertility treatments, stem cell research, and regenerative medicine.<br />This report provides a summary of the congress’s key findings by emphasizing pioneer research and technologies that<br />can influence the future of reproduction and stem cell biology programs. The presence of keynote speakers such as<br />Professor Nicolas Rivron, Mohammad Ebrahim Parsanezhad, Ashraf Moini, Abbas Aflatoonian, Hadi Shafiee, Anna<br />Brini, Omid Camron Farokhzad, and Jeffrey Schweitzer added value to the event, which had over 1100 participants<br />from around the world. While foreign speakers were from various countries Iranian speakers mainly came from Tabriz,<br />Isfahan, Shiraz, Babol, and Tehran that all discussed cutting-edge science and successful disease treatments. To<br />ensure a more comprehensive representation, it is suggested that a wider geographic distribution of national and<br />foreign speakers should be considered in future plan.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="982e64b51828f4e09269c97d6b94432a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117598128,"asset_id":123077647,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117598128/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123077647"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123077647"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123077647; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123077647]").text(description); $(".js-view-count[data-work-id=123077647]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123077647; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123077647']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "982e64b51828f4e09269c97d6b94432a" } } $('.js-work-strip[data-work-id=123077647]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123077647,"title":"Exploring Advances in Reproduction and Stem Cell Biology: Highlights from The 24th and 19th International Congresses in Iran","translated_title":"","metadata":{"doi":"10.22074/CELLJ.2024.2027233.1559","abstract":"The 24th and 19th International Congresses on Reproduction and Stem Cell Biology in the Islamic Republic of Iran brought\ntogether experts and researchers worldwide to explore the latest advancements in these fields. Different topics were\ndiscussed, including such as reproductive health, infertility treatments, stem cell research, and regenerative medicine.\nThis report provides a summary of the congress’s key findings by emphasizing pioneer research and technologies that\ncan influence the future of reproduction and stem cell biology programs. The presence of keynote speakers such as\nProfessor Nicolas Rivron, Mohammad Ebrahim Parsanezhad, Ashraf Moini, Abbas Aflatoonian, Hadi Shafiee, Anna\nBrini, Omid Camron Farokhzad, and Jeffrey Schweitzer added value to the event, which had over 1100 participants\nfrom around the world. While foreign speakers were from various countries Iranian speakers mainly came from Tabriz,\nIsfahan, Shiraz, Babol, and Tehran that all discussed cutting-edge science and successful disease treatments. 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The presence of keynote speakers such as\nProfessor Nicolas Rivron, Mohammad Ebrahim Parsanezhad, Ashraf Moini, Abbas Aflatoonian, Hadi Shafiee, Anna\nBrini, Omid Camron Farokhzad, and Jeffrey Schweitzer added value to the event, which had over 1100 participants\nfrom around the world. While foreign speakers were from various countries Iranian speakers mainly came from Tabriz,\nIsfahan, Shiraz, Babol, and Tehran that all discussed cutting-edge science and successful disease treatments. To\nensure a more comprehensive representation, it is suggested that a wider geographic distribution of national and\nforeign speakers should be considered in future plan.","internal_url":"https://www.academia.edu/123077647/Exploring_Advances_in_Reproduction_and_Stem_Cell_Biology_Highlights_from_The_24th_and_19th_International_Congresses_in_Iran","translated_internal_url":"","created_at":"2024-08-20T23:41:30.306-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42255301,"work_id":123077647,"tagging_user_id":126987384,"tagged_user_id":null,"co_author_invite_id":7523671,"email":"l***n@royan-rc.ac.ir","display_order":1,"name":"Leila Satarian","title":"Exploring Advances in Reproduction and Stem Cell Biology: Highlights from The 24th and 19th International Congresses in Iran"}],"downloadable_attachments":[{"id":117598128,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117598128/thumbnails/1.jpg","file_name":"Cell_J_26_398.pdf","download_url":"https://www.academia.edu/attachments/117598128/download_file","bulk_download_file_name":"Exploring_Advances_in_Reproduction_and_S.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117598128/Cell_J_26_398-libre.pdf?1724224282=\u0026response-content-disposition=attachment%3B+filename%3DExploring_Advances_in_Reproduction_and_S.pdf\u0026Expires=1738725410\u0026Signature=Y-n92mbYM~~Dv3k1sFw36yPI-5hgOhfFt~eEgdAqcy0vT4gbMBG5QfPTSvSGMuyZaUadshIeKa~5q4WWj~DWXERQMsBJZGNe9XsLxUCWVcMrTdttvyjBUYJUQdkmo4t7MqtUuVH1xesvf8UkhVtjUXdtCRHBLq0VIUW-C4BEZCXtr9iKFSX1zV7vQyPMy3FfvgHk9jLQWNI0Q16RtaWYWb~XHwVPbOYdC6Tt3eNM1jRptBvJpnZUWlhdfR3q-7Jf7KdI1UFc9MrcQIsYWmerGKSLK9rejrh13fu1LEFeXy6Ot4z9VvXxXgxEBTpt8EHzrFEMFXKBd8wXVhIdlDQYDg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Exploring_Advances_in_Reproduction_and_Stem_Cell_Biology_Highlights_from_The_24th_and_19th_International_Congresses_in_Iran","translated_slug":"","page_count":5,"language":"en","content_type":"Work","summary":"The 24th and 19th International Congresses on Reproduction and Stem Cell Biology in the Islamic Republic of Iran brought\ntogether experts and researchers worldwide to explore the latest advancements in these fields. Different topics were\ndiscussed, including such as reproductive health, infertility treatments, stem cell research, and regenerative medicine.\nThis report provides a summary of the congress’s key findings by emphasizing pioneer research and technologies that\ncan influence the future of reproduction and stem cell biology programs. The presence of keynote speakers such as\nProfessor Nicolas Rivron, Mohammad Ebrahim Parsanezhad, Ashraf Moini, Abbas Aflatoonian, Hadi Shafiee, Anna\nBrini, Omid Camron Farokhzad, and Jeffrey Schweitzer added value to the event, which had over 1100 participants\nfrom around the world. While foreign speakers were from various countries Iranian speakers mainly came from Tabriz,\nIsfahan, Shiraz, Babol, and Tehran that all discussed cutting-edge science and successful disease treatments. To\nensure a more comprehensive representation, it is suggested that a wider geographic distribution of national and\nforeign speakers should be considered in future plan.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":117598128,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117598128/thumbnails/1.jpg","file_name":"Cell_J_26_398.pdf","download_url":"https://www.academia.edu/attachments/117598128/download_file","bulk_download_file_name":"Exploring_Advances_in_Reproduction_and_S.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117598128/Cell_J_26_398-libre.pdf?1724224282=\u0026response-content-disposition=attachment%3B+filename%3DExploring_Advances_in_Reproduction_and_S.pdf\u0026Expires=1738725410\u0026Signature=Y-n92mbYM~~Dv3k1sFw36yPI-5hgOhfFt~eEgdAqcy0vT4gbMBG5QfPTSvSGMuyZaUadshIeKa~5q4WWj~DWXERQMsBJZGNe9XsLxUCWVcMrTdttvyjBUYJUQdkmo4t7MqtUuVH1xesvf8UkhVtjUXdtCRHBLq0VIUW-C4BEZCXtr9iKFSX1zV7vQyPMy3FfvgHk9jLQWNI0Q16RtaWYWb~XHwVPbOYdC6Tt3eNM1jRptBvJpnZUWlhdfR3q-7Jf7KdI1UFc9MrcQIsYWmerGKSLK9rejrh13fu1LEFeXy6Ot4z9VvXxXgxEBTpt8EHzrFEMFXKBd8wXVhIdlDQYDg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":52048,"name":"Stem Cell Research","url":"https://www.academia.edu/Documents/in/Stem_Cell_Research"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123077610"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123077610/Usher_Syndrome_Type_2_in_An_Iranian_Family_A_Novel_Founder_Variation_in_The_USH2A_Gene"><img alt="Research paper thumbnail of Usher Syndrome Type 2 in An Iranian Family: A Novel Founder Variation in The USH2A Gene" class="work-thumbnail" src="https://attachments.academia-assets.com/117598103/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123077610/Usher_Syndrome_Type_2_in_An_Iranian_Family_A_Novel_Founder_Variation_in_The_USH2A_Gene">Usher Syndrome Type 2 in An Iranian Family: A Novel Founder Variation in The USH2A Gene</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://royaninstitute.academia.edu/CellJournalYakhteh">Cell Journal Yakhteh</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Neissi">Mostafa Neissi</a></span></div><div class="wp-workCard_item"><span>Vol 26, No 6, June </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This study delves into Usher syndrome type 2 (USH2), an uncommon genetic disorder characterized b...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This study delves into Usher syndrome type 2 (USH2), an uncommon genetic disorder characterized by sensorineural hearing loss (HL) and retinitis pigmentosa (RP), often associated with the USH2A gene. Focusing on an Iranian family exhibiting USH2 symptoms, exome-sequencing was employed for a comprehensive genome analysis in a 30-yearold patient. The investigation unveiled a novel variation (NM_206933.4: c.9389G>A; p.Trp3130*) within exon 48 of the USH2A gene, a previously unreported variant emphasizing the genetic diversity in USH2. Sanger sequencing was then utilized to assess variation segregation within the family, offering insights into the inheritance pattern. This discovery not only advances our understanding of the genetic basis of USH2 but also holds significant implications for genetic counseling, early management, and informed decision-making regarding prenatal options. By adopting an integrated approach, this study aims to empower affected families, facilitating a nuanced understanding of the disorder's complexities and ultimately improving patient outcomes and family well-being through informed decisionmaking and proactive management strategies.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7769c1248c0c11c3b6092c55ce6cadd8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117598103,"asset_id":123077610,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117598103/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123077610"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123077610"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123077610; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123077610]").text(description); $(".js-view-count[data-work-id=123077610]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123077610; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123077610']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7769c1248c0c11c3b6092c55ce6cadd8" } } $('.js-work-strip[data-work-id=123077610]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123077610,"title":"Usher Syndrome Type 2 in An Iranian Family: A Novel Founder Variation in The USH2A Gene","translated_title":"","metadata":{"doi":"10.22074/CELLJ.2024.2024223.1521","abstract":"This study delves into Usher syndrome type 2 (USH2), an uncommon genetic disorder characterized by sensorineural hearing loss (HL) and retinitis pigmentosa (RP), often associated with the USH2A gene. Focusing on an Iranian family exhibiting USH2 symptoms, exome-sequencing was employed for a comprehensive genome analysis in a 30-yearold patient. The investigation unveiled a novel variation (NM_206933.4: c.9389G\u003eA; p.Trp3130*) within exon 48 of the USH2A gene, a previously unreported variant emphasizing the genetic diversity in USH2. Sanger sequencing was then utilized to assess variation segregation within the family, offering insights into the inheritance pattern. This discovery not only advances our understanding of the genetic basis of USH2 but also holds significant implications for genetic counseling, early management, and informed decision-making regarding prenatal options. By adopting an integrated approach, this study aims to empower affected families, facilitating a nuanced understanding of the disorder's complexities and ultimately improving patient outcomes and family well-being through informed decisionmaking and proactive management strategies.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Vol 26, No 6, June "},"translated_abstract":"This study delves into Usher syndrome type 2 (USH2), an uncommon genetic disorder characterized by sensorineural hearing loss (HL) and retinitis pigmentosa (RP), often associated with the USH2A gene. Focusing on an Iranian family exhibiting USH2 symptoms, exome-sequencing was employed for a comprehensive genome analysis in a 30-yearold patient. The investigation unveiled a novel variation (NM_206933.4: c.9389G\u003eA; p.Trp3130*) within exon 48 of the USH2A gene, a previously unreported variant emphasizing the genetic diversity in USH2. Sanger sequencing was then utilized to assess variation segregation within the family, offering insights into the inheritance pattern. This discovery not only advances our understanding of the genetic basis of USH2 but also holds significant implications for genetic counseling, early management, and informed decision-making regarding prenatal options. By adopting an integrated approach, this study aims to empower affected families, facilitating a nuanced understanding of the disorder's complexities and ultimately improving patient outcomes and family well-being through informed decisionmaking and proactive management strategies.","internal_url":"https://www.academia.edu/123077610/Usher_Syndrome_Type_2_in_An_Iranian_Family_A_Novel_Founder_Variation_in_The_USH2A_Gene","translated_internal_url":"","created_at":"2024-08-20T23:40:40.107-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42255297,"work_id":123077610,"tagging_user_id":126987384,"tagged_user_id":112722747,"co_author_invite_id":null,"email":"i***i@gmail.com","display_order":1,"name":"Mostafa Neissi","title":"Usher Syndrome Type 2 in An Iranian Family: A Novel Founder Variation in The USH2A Gene"}],"downloadable_attachments":[{"id":117598103,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117598103/thumbnails/1.jpg","file_name":"Cell_J_26_392.pdf","download_url":"https://www.academia.edu/attachments/117598103/download_file","bulk_download_file_name":"Usher_Syndrome_Type_2_in_An_Iranian_Fami.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117598103/Cell_J_26_392-libre.pdf?1724224288=\u0026response-content-disposition=attachment%3B+filename%3DUsher_Syndrome_Type_2_in_An_Iranian_Fami.pdf\u0026Expires=1738763676\u0026Signature=VDhvjunmWzwEZIx4pm9ifvr~cG8AgHptRl46Kp8U4-7~J3dv81Xs2wpFQXVawgMt9HH04faOwtJQ9ifjeLN~72-8KGKkgDAhuYpvH8T7IH1l6kZJrmWitaWzZNF-0hWTqb-8BlxOn0TZCIoYNzJ4eyPaNnrtWNmqURN6r5DzTlo5OitDqSqKYV99T~A18LD40-wsAywlQHXd1Z9~DSMjNJXy6tXJLv7AE2ckQgzF4Ssnqi0xoInUMr6-wbL8eEfWJikoFPuASx8TxiYRmo-YKrsPM3DjPLda2aRigeWQAITgeqjHn9MKSLM7dxrLfSDZETL70Gb0v~yNtNf~aXUlYg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Usher_Syndrome_Type_2_in_An_Iranian_Family_A_Novel_Founder_Variation_in_The_USH2A_Gene","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"This study delves into Usher syndrome type 2 (USH2), an uncommon genetic disorder characterized by sensorineural hearing loss (HL) and retinitis pigmentosa (RP), often associated with the USH2A gene. Focusing on an Iranian family exhibiting USH2 symptoms, exome-sequencing was employed for a comprehensive genome analysis in a 30-yearold patient. The investigation unveiled a novel variation (NM_206933.4: c.9389G\u003eA; p.Trp3130*) within exon 48 of the USH2A gene, a previously unreported variant emphasizing the genetic diversity in USH2. Sanger sequencing was then utilized to assess variation segregation within the family, offering insights into the inheritance pattern. This discovery not only advances our understanding of the genetic basis of USH2 but also holds significant implications for genetic counseling, early management, and informed decision-making regarding prenatal options. By adopting an integrated approach, this study aims to empower affected families, facilitating a nuanced understanding of the disorder's complexities and ultimately improving patient outcomes and family well-being through informed decisionmaking and proactive management strategies.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":117598103,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117598103/thumbnails/1.jpg","file_name":"Cell_J_26_392.pdf","download_url":"https://www.academia.edu/attachments/117598103/download_file","bulk_download_file_name":"Usher_Syndrome_Type_2_in_An_Iranian_Fami.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117598103/Cell_J_26_392-libre.pdf?1724224288=\u0026response-content-disposition=attachment%3B+filename%3DUsher_Syndrome_Type_2_in_An_Iranian_Fami.pdf\u0026Expires=1738763676\u0026Signature=VDhvjunmWzwEZIx4pm9ifvr~cG8AgHptRl46Kp8U4-7~J3dv81Xs2wpFQXVawgMt9HH04faOwtJQ9ifjeLN~72-8KGKkgDAhuYpvH8T7IH1l6kZJrmWitaWzZNF-0hWTqb-8BlxOn0TZCIoYNzJ4eyPaNnrtWNmqURN6r5DzTlo5OitDqSqKYV99T~A18LD40-wsAywlQHXd1Z9~DSMjNJXy6tXJLv7AE2ckQgzF4Ssnqi0xoInUMr6-wbL8eEfWJikoFPuASx8TxiYRmo-YKrsPM3DjPLda2aRigeWQAITgeqjHn9MKSLM7dxrLfSDZETL70Gb0v~yNtNf~aXUlYg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1225171,"name":"Syndrome","url":"https://www.academia.edu/Documents/in/Syndrome"},{"id":1824390,"name":"Usher","url":"https://www.academia.edu/Documents/in/Usher"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123077579"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123077579/Tramadol_Influences_Cellular_Metabolism_and_Autophagy_in_Germ_and_Sertoli_Cells_Exercise_Training_as_A_Potential_Ameliorative_Strategy"><img alt="Research paper thumbnail of Tramadol Influences Cellular Metabolism and Autophagy in Germ and Sertoli Cells; Exercise Training as A Potential Ameliorative Strategy" class="work-thumbnail" src="https://attachments.academia-assets.com/117598082/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123077579/Tramadol_Influences_Cellular_Metabolism_and_Autophagy_in_Germ_and_Sertoli_Cells_Exercise_Training_as_A_Potential_Ameliorative_Strategy">Tramadol Influences Cellular Metabolism and Autophagy in Germ and Sertoli Cells; Exercise Training as A Potential Ameliorative Strategy</a></div><div class="wp-workCard_item"><span>Vol 26, No 6, June</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Dysregulation of lipid and carbohydrate/fatty acid (FA) balance in Sertoli and germ ce...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Dysregulation of lipid and carbohydrate/fatty acid (FA) balance in Sertoli and germ cells alters the NADP + / NADPH ratio, resulting in metabolic autophagy in testicles. Tramadol (TRA) adversely affects spermatogenesis development, and it is not reversed within short periods of time after withdrawal. Therefore, the present study aimed to examine the boosting effect of different exercise training protocols (ETPs) on TRA-induced detrimental effects after withdrawal. Materials and Methods: In this experimental study, 36 mature Wistar rats were separated into control and TRAsole (administered 40 mg/kg of TRA and euthanized 60 days after TRA administration), Con-TRA (stopped TRA administration after 60 days, and continued for additional 60 days after withdrawal), TRA+low-intensity (TRA+LICT), TRA + moderate-intensity (TRA+MICT), and TRA+high-intensity continuous (TRA+HICT) ETPs-induced groups (n=6/ group, ETPs were initiated for 60 days after stopping TRA administration). Next, the intracytoplasmic carbohydrate and lipids/FAs content, testicular lactate and lactate dehydrogenase (LDH) levels, relative ratios of NADP + /NADPH, serum testosterone levels, and the Leydig cells steroidogenic activity, the mRNA levels of Beclin-1, p62, LC3-I, and Atg7 as well as the LC3-I/II + germ and somatic cells mean distributions were analyzed. Results: The LICT and MICT could ameliorate the TRA-induced carbohydrates/lipids, FAs imbalance, increase lactate, LDH and testosterone levels, re-balance the NADP + /NADPH ratio, and reregulate the autophagy and steroidogenic activities in the Leydig and Sertoli cells. Conclusion: Collectively, LICT and MICT can ameliorate the TRA-induced metabolic-oxidative autophagy by rebalancing energy survey in testicles and down-regulating autophagy reactions in Sertoli cells and rebalancing it in the Leydig cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="839c54782b2115fc9adbd494404b734d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117598082,"asset_id":123077579,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117598082/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123077579"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123077579"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123077579; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123077579]").text(description); $(".js-view-count[data-work-id=123077579]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123077579; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123077579']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "839c54782b2115fc9adbd494404b734d" } } $('.js-work-strip[data-work-id=123077579]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123077579,"title":"Tramadol Influences Cellular Metabolism and Autophagy in Germ and Sertoli Cells; Exercise Training as A Potential Ameliorative Strategy","translated_title":"","metadata":{"doi":"10.22074/CELLJ.2024.2024852.1532","abstract":"Objective: Dysregulation of lipid and carbohydrate/fatty acid (FA) balance in Sertoli and germ cells alters the NADP + / NADPH ratio, resulting in metabolic autophagy in testicles. Tramadol (TRA) adversely affects spermatogenesis development, and it is not reversed within short periods of time after withdrawal. Therefore, the present study aimed to examine the boosting effect of different exercise training protocols (ETPs) on TRA-induced detrimental effects after withdrawal. Materials and Methods: In this experimental study, 36 mature Wistar rats were separated into control and TRAsole (administered 40 mg/kg of TRA and euthanized 60 days after TRA administration), Con-TRA (stopped TRA administration after 60 days, and continued for additional 60 days after withdrawal), TRA+low-intensity (TRA+LICT), TRA + moderate-intensity (TRA+MICT), and TRA+high-intensity continuous (TRA+HICT) ETPs-induced groups (n=6/ group, ETPs were initiated for 60 days after stopping TRA administration). Next, the intracytoplasmic carbohydrate and lipids/FAs content, testicular lactate and lactate dehydrogenase (LDH) levels, relative ratios of NADP + /NADPH, serum testosterone levels, and the Leydig cells steroidogenic activity, the mRNA levels of Beclin-1, p62, LC3-I, and Atg7 as well as the LC3-I/II + germ and somatic cells mean distributions were analyzed. Results: The LICT and MICT could ameliorate the TRA-induced carbohydrates/lipids, FAs imbalance, increase lactate, LDH and testosterone levels, re-balance the NADP + /NADPH ratio, and reregulate the autophagy and steroidogenic activities in the Leydig and Sertoli cells. Conclusion: Collectively, LICT and MICT can ameliorate the TRA-induced metabolic-oxidative autophagy by rebalancing energy survey in testicles and down-regulating autophagy reactions in Sertoli cells and rebalancing it in the Leydig cells.","ai_title_tag":"Tramadol Effects on Germ Cells: Exercise Training Benefits","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Vol 26, No 6, June"},"translated_abstract":"Objective: Dysregulation of lipid and carbohydrate/fatty acid (FA) balance in Sertoli and germ cells alters the NADP + / NADPH ratio, resulting in metabolic autophagy in testicles. Tramadol (TRA) adversely affects spermatogenesis development, and it is not reversed within short periods of time after withdrawal. Therefore, the present study aimed to examine the boosting effect of different exercise training protocols (ETPs) on TRA-induced detrimental effects after withdrawal. Materials and Methods: In this experimental study, 36 mature Wistar rats were separated into control and TRAsole (administered 40 mg/kg of TRA and euthanized 60 days after TRA administration), Con-TRA (stopped TRA administration after 60 days, and continued for additional 60 days after withdrawal), TRA+low-intensity (TRA+LICT), TRA + moderate-intensity (TRA+MICT), and TRA+high-intensity continuous (TRA+HICT) ETPs-induced groups (n=6/ group, ETPs were initiated for 60 days after stopping TRA administration). Next, the intracytoplasmic carbohydrate and lipids/FAs content, testicular lactate and lactate dehydrogenase (LDH) levels, relative ratios of NADP + /NADPH, serum testosterone levels, and the Leydig cells steroidogenic activity, the mRNA levels of Beclin-1, p62, LC3-I, and Atg7 as well as the LC3-I/II + germ and somatic cells mean distributions were analyzed. Results: The LICT and MICT could ameliorate the TRA-induced carbohydrates/lipids, FAs imbalance, increase lactate, LDH and testosterone levels, re-balance the NADP + /NADPH ratio, and reregulate the autophagy and steroidogenic activities in the Leydig and Sertoli cells. Conclusion: Collectively, LICT and MICT can ameliorate the TRA-induced metabolic-oxidative autophagy by rebalancing energy survey in testicles and down-regulating autophagy reactions in Sertoli cells and rebalancing it in the Leydig cells.","internal_url":"https://www.academia.edu/123077579/Tramadol_Influences_Cellular_Metabolism_and_Autophagy_in_Germ_and_Sertoli_Cells_Exercise_Training_as_A_Potential_Ameliorative_Strategy","translated_internal_url":"","created_at":"2024-08-20T23:39:20.895-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42255291,"work_id":123077579,"tagging_user_id":126987384,"tagged_user_id":null,"co_author_invite_id":7793666,"email":"j***r@urmia.ac.ir","display_order":1,"name":"Javad Tolouei Azar","title":"Tramadol Influences Cellular Metabolism and Autophagy in Germ and Sertoli Cells; Exercise Training as A Potential Ameliorative Strategy"}],"downloadable_attachments":[{"id":117598082,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117598082/thumbnails/1.jpg","file_name":"Cell_J_26_380.pdf","download_url":"https://www.academia.edu/attachments/117598082/download_file","bulk_download_file_name":"Tramadol_Influences_Cellular_Metabolism.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117598082/Cell_J_26_380-libre.pdf?1724224301=\u0026response-content-disposition=attachment%3B+filename%3DTramadol_Influences_Cellular_Metabolism.pdf\u0026Expires=1738763676\u0026Signature=C8QxS2mdf~mwr0HuqZ-R0xuZPUJzs4sp0TelgCzszCnmccfnAWdkarU5kwNaLhKFHyvzXgoPzaex4pl9Em5zRyvE5d1wFsUNxLHo3P2lFtryKuNfJ~1anjN9XUUwaa7b75bscRBSL~UWz3ins12TKgXJi3KurbNPzcDj4NpsYXOCAE-OdOmwAGK3iATlaa6lNnE~sby3fAlq9KRWVpmAj8lTMLtnpp5dGDXXrIv7HIao0oxLXMQB-RbTq0VxqDcbbVwAL4GgblVsHJC1GiAUw5sJYVhRES4rxZCsMr3oxK8RAS8jvT256qMeawoXv16Engs4QjDUnnbCz0p3Q349GA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Tramadol_Influences_Cellular_Metabolism_and_Autophagy_in_Germ_and_Sertoli_Cells_Exercise_Training_as_A_Potential_Ameliorative_Strategy","translated_slug":"","page_count":12,"language":"en","content_type":"Work","summary":"Objective: Dysregulation of lipid and carbohydrate/fatty acid (FA) balance in Sertoli and germ cells alters the NADP + / NADPH ratio, resulting in metabolic autophagy in testicles. Tramadol (TRA) adversely affects spermatogenesis development, and it is not reversed within short periods of time after withdrawal. Therefore, the present study aimed to examine the boosting effect of different exercise training protocols (ETPs) on TRA-induced detrimental effects after withdrawal. Materials and Methods: In this experimental study, 36 mature Wistar rats were separated into control and TRAsole (administered 40 mg/kg of TRA and euthanized 60 days after TRA administration), Con-TRA (stopped TRA administration after 60 days, and continued for additional 60 days after withdrawal), TRA+low-intensity (TRA+LICT), TRA + moderate-intensity (TRA+MICT), and TRA+high-intensity continuous (TRA+HICT) ETPs-induced groups (n=6/ group, ETPs were initiated for 60 days after stopping TRA administration). Next, the intracytoplasmic carbohydrate and lipids/FAs content, testicular lactate and lactate dehydrogenase (LDH) levels, relative ratios of NADP + /NADPH, serum testosterone levels, and the Leydig cells steroidogenic activity, the mRNA levels of Beclin-1, p62, LC3-I, and Atg7 as well as the LC3-I/II + germ and somatic cells mean distributions were analyzed. Results: The LICT and MICT could ameliorate the TRA-induced carbohydrates/lipids, FAs imbalance, increase lactate, LDH and testosterone levels, re-balance the NADP + /NADPH ratio, and reregulate the autophagy and steroidogenic activities in the Leydig and Sertoli cells. Conclusion: Collectively, LICT and MICT can ameliorate the TRA-induced metabolic-oxidative autophagy by rebalancing energy survey in testicles and down-regulating autophagy reactions in Sertoli cells and rebalancing it in the Leydig cells.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":117598082,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117598082/thumbnails/1.jpg","file_name":"Cell_J_26_380.pdf","download_url":"https://www.academia.edu/attachments/117598082/download_file","bulk_download_file_name":"Tramadol_Influences_Cellular_Metabolism.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117598082/Cell_J_26_380-libre.pdf?1724224301=\u0026response-content-disposition=attachment%3B+filename%3DTramadol_Influences_Cellular_Metabolism.pdf\u0026Expires=1738763676\u0026Signature=C8QxS2mdf~mwr0HuqZ-R0xuZPUJzs4sp0TelgCzszCnmccfnAWdkarU5kwNaLhKFHyvzXgoPzaex4pl9Em5zRyvE5d1wFsUNxLHo3P2lFtryKuNfJ~1anjN9XUUwaa7b75bscRBSL~UWz3ins12TKgXJi3KurbNPzcDj4NpsYXOCAE-OdOmwAGK3iATlaa6lNnE~sby3fAlq9KRWVpmAj8lTMLtnpp5dGDXXrIv7HIao0oxLXMQB-RbTq0VxqDcbbVwAL4GgblVsHJC1GiAUw5sJYVhRES4rxZCsMr3oxK8RAS8jvT256qMeawoXv16Engs4QjDUnnbCz0p3Q349GA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":213909,"name":"Sertoli cells","url":"https://www.academia.edu/Documents/in/Sertoli_cells"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="9747542" id="papers"><div class="js-work-strip profile--work_container" data-work-id="127680381"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680381/Cell_Based_Therapy_for_Cerebral_Palsy_A_Puzzle_in_Progress"><img alt="Research paper thumbnail of Cell-Based Therapy for Cerebral Palsy: A Puzzle in Progress" class="work-thumbnail" src="https://attachments.academia-assets.com/121377935/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680381/Cell_Based_Therapy_for_Cerebral_Palsy_A_Puzzle_in_Progress">Cell-Based Therapy for Cerebral Palsy: A Puzzle in Progress</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 9, September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cell-based therapy has shown promising outcomes in the treatment of cerebral palsy (CP). However,...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cell-based therapy has shown promising outcomes in the treatment of cerebral palsy (CP). However, there is no consensus on a standard therapeutic protocol regarding the source of cells, optimal cell dose, timing and frequency of cell injections, route of administration, or the use of combination therapy. This lack of consensus necessitates a comprehensive investigation to clarify these crucial yet undefined factors in cell-based therapy for CP patients. In this commentary, we discuss and compare the trends in Gross Motor Function Measure-66 following intrathecal injection of umbilical cord blood mononuclear cells (UCB-MNCs) and umbilical cord tissue mesenchymal stromal cells (UCT-MSCs) in children with CP. Our study revealed that MNC injections led to earlier improvements in gross motor function, whereas MSC applications resulted in more sustainable changes. These findings provide key insights into the efficacy of different cell types, which will be beneficial for future studies and for refining cell-based therapy protocols for CP treatment.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f4392af34cd9945f4b29991691d11029" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377935,"asset_id":127680381,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377935/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680381"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680381"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680381; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680381]").text(description); $(".js-view-count[data-work-id=127680381]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680381; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680381']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f4392af34cd9945f4b29991691d11029" } } $('.js-work-strip[data-work-id=127680381]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680381,"title":"Cell-Based Therapy for Cerebral Palsy: A Puzzle in Progress","internal_url":"https://www.academia.edu/127680381/Cell_Based_Therapy_for_Cerebral_Palsy_A_Puzzle_in_Progress","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377935,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377935/thumbnails/1.jpg","file_name":"Cell_J_26_569.pdf","download_url":"https://www.academia.edu/attachments/121377935/download_file","bulk_download_file_name":"Cell_Based_Therapy_for_Cerebral_Palsy_A.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377935/Cell_J_26_569-libre.pdf?1739691820=\u0026response-content-disposition=attachment%3B+filename%3DCell_Based_Therapy_for_Cerebral_Palsy_A.pdf\u0026Expires=1740152615\u0026Signature=MEe141oz96hwQH8pxGg8tCvIuuOPgI7y8J~YAgd1G6DeCS6tFT7Dmkelfn303r9dYDTzaJO23wwb3LlpxEdMO-8MjfmZXjAN-8zVxy3hJqYfTzm7yln141zXMtJmYdrC5Z6dIV743AKj2q-hxOtgJmMqnth4IfyGd6rArMBXToxf~SBt-EP1GYr-QqfNMF5yghFE7Zqa6MpcfpaX09MGmYwjUYfnwNpKu5tZ3ff7kc4l408-BhDNbyHe5NZZCSSjg~0ryY6uYLKikQKIp9K~8TJ9iB~OK4uA3RqUJB31t3qNHQJ7CkOF67iNPECajBI9IGRdMfK7Qh0tFAK29Gm~KA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680370"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680370/The_Role_of_Quercetin_and_Exercise_in_Modulating_Apoptosis_and_Cardiomyopathy_via_PI3K_AKT_FOXO3_Pathways_in_Diabetic_Obese_Rats"><img alt="Research paper thumbnail of The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats" class="work-thumbnail" src="https://attachments.academia-assets.com/121377924/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680370/The_Role_of_Quercetin_and_Exercise_in_Modulating_Apoptosis_and_Cardiomyopathy_via_PI3K_AKT_FOXO3_Pathways_in_Diabetic_Obese_Rats">The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 9, September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Quercetin and exercise both have antidiabetic effects through decreasing blood glucose...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Quercetin and exercise both have antidiabetic effects through decreasing blood glucose while increasing insulin sensitivity. Therefore, the present study aimed to investigate the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) exercises along with quercetin administration on apoptosis and cardiomyopathy in diabetic obese rats. Materials and Methods: In this experimental study, 35 male Wistar rats [diabetic rats for experimental groups and normal rats for healthy control (HC)] were divided into seven groups (for each group n=5): HC, diabetic control (DC), diabetic quercetin control (DQC), diabetic HIIT (DHT), diabetic MICT (DMT), DHT with quercetin (DQHT) and DMT with quercetin (DQMT). The rats were fed a high-fat diet (HFD) for 8 weeks and a low dose of streptozotocin (STZ) was administered to create a model of type 2 diabetes mellitus (T2DM). Eight weeks of HIIT and MICT with or without quercetin treatment were performed. Quercetin was used at 15 mg/kg, as a suspension in carboxymethyl cellulose (CMC) at a concentration of 0.5%. One-way analysis of variance with LSD's post-hoc test with a significant level of P≤0.05 was used to analyze data. Results: Just 8 weeks of HIIT and MICT protected the protein content of PI3K, AKT, and FOXO3 and caspase-8 (Casp-8) gene expression in heart tissues (P<0.05). Quercetin and both training protocols decreased blood glucose, while improving inflammatory markers and the lipid profile (P<0.05). Conclusion: Reduction in blood glucose along with improvements in the inflammatory markers and the lipid profile by quercetin injection may be a promising approach for the development of new antidiabetic medications. In addition, both training protocols showed potentially successful diabetic cardiomyopathy treatments through modulating the FOXO3 and PI3K/AKT pathways.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dbb9021d68833365a24ed2185d94d499" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377924,"asset_id":127680370,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377924/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680370"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680370"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680370; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680346"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680346/Interleukin_12_Inhibits_Tumor_Growth_and_Metastasis_Promoted_by_Tumor_Associated_Mesenchymal_Stem_Cells_in_Triple_Negative_Breast_Cancer"><img alt="Research paper thumbnail of Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer" class="work-thumbnail" src="https://attachments.academia-assets.com/121377905/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680346/Interleukin_12_Inhibits_Tumor_Growth_and_Metastasis_Promoted_by_Tumor_Associated_Mesenchymal_Stem_Cells_in_Triple_Negative_Breast_Cancer">Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 9, September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: The aim of this study was to understand the interactions between tumor-associated mese...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: The aim of this study was to understand the interactions between tumor-associated mesenchymal stem cells (TA-MSCs) and triple-negative breast cancer (TNBC) cells, which appear to be necessary for developing effective therapies. Materials and Methods: In this experimental study, MDA-MB-231 and 4T1 TNBC cells were co-cultured with bone marrow-derived MSCs, and TA-MSCs conditioned media (CM) were collected. TA-MSC CM-treated TNBC cells were subjected to migration and invasion assays. Epithelial-mesenchymal transition (EMT) marker expression was quantified by real-time polymerase chain reaction (RT-PCR). Cell proliferation was measured using trypan blue exclusion technique, while cell cycle distribution and apoptosis were assessed by flow cytometry. The effects of TA-MSCs on tumor volume, survival rate, and lung metastasis were evaluated by subcutaneous co-injection of MSCs with 4T1 cells in the right flanks of BALB/c mice (n=5 per group). Intratumoral interleukin-12 (IL-12) immunotherapy was performed using lentiviral particles as a rescue experiment. The TA-MSCs RNA-seq dataset (PRJEB27694) was analyzed to detect elevated metastasis-associated oncogenes, downloaded from the European Nucleotide Archive database. For validation of the RNA-seq data analysis, the expression levels of candidate oncogenes were evaluated in TA-MSCs, TNBC cells, and tumor tissue using RT-PCR. Results: TA-MSCs enhanced migration, invasion, and EMT of TNBC cells in vitro without affecting cell proliferation or apoptosis. In vivo, TA-MSCs increased tumor growth and lung metastasis, while decreasing survival rates. IL-12 therapy elevated serum IL-12 and interferon-gamma (IFN-γ) expression, suppressed tumor volume and lung metastasis, and improved overall survival in the TA-MSC group. RNA-seq data analysis identified upregulated oncogenes in TA-MSCs, among which MMP3, CXCL2, CXCL5, and ICAM1 were selected as the most relevant to metastasis. These genes showed increased expression in TA-MSCs, TNBC cells, and tumor tissues. Conclusion: The findings of the present study revealed a complex interplay between TA-MSCs and TNBC cells that affects tumor growth and metastasis. Preclinical results indicate that intratumoral IL-12 immunotherapy shows promise in overcoming TA-MSC-promoted tumor growth and metastasis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3b01d4214d35ef6f8609a763c2e2fad8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377905,"asset_id":127680346,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377905/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680346"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680346"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680346; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680346]").text(description); $(".js-view-count[data-work-id=127680346]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680346; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680346']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3b01d4214d35ef6f8609a763c2e2fad8" } } $('.js-work-strip[data-work-id=127680346]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680346,"title":"Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer","internal_url":"https://www.academia.edu/127680346/Interleukin_12_Inhibits_Tumor_Growth_and_Metastasis_Promoted_by_Tumor_Associated_Mesenchymal_Stem_Cells_in_Triple_Negative_Breast_Cancer","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377905,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377905/thumbnails/1.jpg","file_name":"Cell_J_26_543.pdf","download_url":"https://www.academia.edu/attachments/121377905/download_file","bulk_download_file_name":"Interleukin_12_Inhibits_Tumor_Growth_and.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377905/Cell_J_26_543-libre.pdf?1739687743=\u0026response-content-disposition=attachment%3B+filename%3DInterleukin_12_Inhibits_Tumor_Growth_and.pdf\u0026Expires=1739699916\u0026Signature=eFcl5L5agObiiDMMV3G56JsLj6zVx51pLcKoV3ftBFwqBph2zaOi-w0lPJF-MFTL0wslKDj8X5P1TQ~YK4MdjDJ-UDIB7zCWrsZnpzGZ93N7JdR3hboZ0vd~LQUdvSu2Bsqz5s9RwYLpk9NW8ixLT6gNjPkwqLovT941NjiaQAypITCehDffNBMKbmkbzpK6ZXm851~vfBGvE~UlkebllvHQny4mBcqWhD-vaiVczT6ms~9ayDWs5A7WboLmk23MH2bVv6kjW5N0Z6dikJVyiYyeMK3-6V0AK1iGD1Q9zU2pmrfe6bWVxdvmPVmpJ1qfPVLgruJT8aBn8kDD5ZZbPw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680324"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680324/Oxaliplatin_Loaded_Chitosan_Nanoparticles_Decorated_with_Cetuximab_Single_Chain_Variable_Fragment_for_Human_Colorectal_Cancer_Treatment"><img alt="Research paper thumbnail of Oxaliplatin-Loaded Chitosan Nanoparticles Decorated with Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment" class="work-thumbnail" src="https://attachments.academia-assets.com/121377880/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680324/Oxaliplatin_Loaded_Chitosan_Nanoparticles_Decorated_with_Cetuximab_Single_Chain_Variable_Fragment_for_Human_Colorectal_Cancer_Treatment">Oxaliplatin-Loaded Chitosan Nanoparticles Decorated with Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment</a></div><div class="wp-workCard_item"><span> Volume 26, Issue 9 , September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide. Engineered biomolecules can be used as a targeted tool to deliver drugs directly to tumors that reduce the adverse effects of conventional treatments. We aimed to prepare non-targeted oxaliplatin-loaded chitosan nanoparticles (OXPT-CS NPs) and targeted OXPT-CS NPs decorated with cetuximab single-chain variable fragment (scFv) to send both NPs to epidermal growth factor receptor (EGFR) overexpressing HCT 116 cells, a human colorectal carcinoma cell line, for comparing their cytotoxicity. Materials and Methods: In this experimental study, OXPT-CS NPs were synthesized using a fluid system. Encapsulation efficiency percentage (EE%) and oxaliplatin release rate were evaluated. Western blot and cell-based ELISA confirmed scFv production and its binding ability to EGFR, respectively. The Fourier transform infrared spectroscopy (FTIR) determined the conjugation of scFv to OXPT-CS NPs. The NPs were characterized, and their toxicity against the HCT 116 cells was evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and flow cytometry assays. Results: The EE% of OXPT-CS NPs was 93%, and the average diameters were 75.85 ± 8.81 nm and 92.48 ± 9.51 before and after scFv conjugation, respectively. The scFv was purified via affinity chromatography. The western blot method and cell-based ELISA revealed successful purification of scFv and its attachment to EGFR on HCT 116 cells. The FTIR analysis determined the interactions between the scFv and OXPT-CS NPs. According to MTT and flow cytometry results, the targeted delivery system significantly reduced HCT 116 cancer cell viability and increased apoptosis induction up to 99.8%. Conclusion: The scFv-OXPT-CS NPs demonstrated an increased cytotoxic function due to the presence of scFv in its formulation. This delivery system offers a promising method for delivering chemotherapy drugs to cancer cells. More research is needed on the best strategies for improving treatment efficacy by targeting cancer cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2098d1f55b4f71e4f0eda9ca0dbec763" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377880,"asset_id":127680324,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377880/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680324"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680324"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680324; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680324]").text(description); $(".js-view-count[data-work-id=127680324]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680324; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680324']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2098d1f55b4f71e4f0eda9ca0dbec763" } } $('.js-work-strip[data-work-id=127680324]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680324,"title":"Oxaliplatin-Loaded Chitosan Nanoparticles Decorated with Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment","internal_url":"https://www.academia.edu/127680324/Oxaliplatin_Loaded_Chitosan_Nanoparticles_Decorated_with_Cetuximab_Single_Chain_Variable_Fragment_for_Human_Colorectal_Cancer_Treatment","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377880,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377880/thumbnails/1.jpg","file_name":"Cell_J_26_530.pdf","download_url":"https://www.academia.edu/attachments/121377880/download_file","bulk_download_file_name":"Oxaliplatin_Loaded_Chitosan_Nanoparticle.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377880/Cell_J_26_530-libre.pdf?1739687738=\u0026response-content-disposition=attachment%3B+filename%3DOxaliplatin_Loaded_Chitosan_Nanoparticle.pdf\u0026Expires=1739699916\u0026Signature=abbbkqNFBPLtBp1R-LtzZoUvrVtbZGqePrOXzjmOq-szAdrgYEVCkuRmOP3KzYGr9XmIZLkJjt75XNMVuXte0Nv4Rwr3uBNSc1aTxs4P~-JcFetSZKQswxU5uTit3-cOOUUyXB3d5LWG75og3HUYgAmj~i6dikos-jdYhZe5NaZd3w6H6vv87lYRwp1x1gPJ22L8~jkbsCsVSdM3aMozQIT1yX7cdEPQe1m2mgA6zSwIuulHpw3jC8TxIX-xveJWdRyPr6AWilry72F9IrPiAa1EsFNnYBvUUKMCEo1SzSo4mg0HbrSfjPvtDg6dqIpdf4kxk5dd76D9ZfQXhsC4GA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680291"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680291/The_Geographical_Distribution_of_Global_Biobanks"><img alt="Research paper thumbnail of The Geographical Distribution of Global Biobanks" class="work-thumbnail" src="https://attachments.academia-assets.com/121377846/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680291/The_Geographical_Distribution_of_Global_Biobanks">The Geographical Distribution of Global Biobanks</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 9, September</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This study aimed to comprehensively review the global biobanks to visualize their geographical di...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This study aimed to comprehensively review the global biobanks to visualize their geographical distribution. The protocol for this review consisted of the following steps: i. Developing a search strategy to identify biobanks from each continent, ii. Defining variables (such as tissue-based, cell-based, and gene-based biobanks) and organizing them in Excel sheets for data collection, iii. Collecting data, iv. Removing duplicate and invalid entries, v. Structuring the database, and vi. Analyzing the data. MATLAB software was utilized for data analysis and chart plotting. Data on global biobanks aimed to collected through targeted searches of databases, publications, and registries using predefined variables such as biobank type, location, and accessibility. The data were organized, cleaned to remove duplicates, and analyzed using MATLAB to visualize geographical distribution and prevalence patterns. Tissue and cell-based, tissue-based, and cellbased biobanks were the most common type of global biobanks with a prevalence of 30.4, 27.93, and 25.15%. United Kingdom (n=78, P=43.09%), Canada (n=43, P=23.75%), and the United States (n=33, P=18.23%) were the countries with a higher frequency of tissue-based biobanks (domain frequency: 1-78; 0.55-43.09%). However, tissue and genebased biobanks had the most minor frequency and were only in two countries of Spain (n=1, P=25%) and the United Kingdom (n=3, P=75%). The results of this study indicate that the feasibility of designing and conducting biobanks varies by type. Tissue and cell-based biobanks were found to be more prevalent, followed by tissue-based, cell-based, cell and gene-based, tissue, cell, and gene-based, gene-based, and finally, tissue and gene-based biobanks. This study represents the initial step in creating a global database by identifying all types of biobanks worldwide.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8f5ff159a07dd65036d206dcdfebb7bb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377846,"asset_id":127680291,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377846/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680291"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680291"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680291; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680291]").text(description); $(".js-view-count[data-work-id=127680291]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680291; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680291']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8f5ff159a07dd65036d206dcdfebb7bb" } } $('.js-work-strip[data-work-id=127680291]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680291,"title":"The Geographical Distribution of Global Biobanks","internal_url":"https://www.academia.edu/127680291/The_Geographical_Distribution_of_Global_Biobanks","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377846,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377846/thumbnails/1.jpg","file_name":"Cell_J_26_523.pdf","download_url":"https://www.academia.edu/attachments/121377846/download_file","bulk_download_file_name":"The_Geographical_Distribution_of_Global.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377846/Cell_J_26_523-libre.pdf?1739687731=\u0026response-content-disposition=attachment%3B+filename%3DThe_Geographical_Distribution_of_Global.pdf\u0026Expires=1740152616\u0026Signature=aZV16RM2eOQn8esajtAevApYRQB3KArVshU7ERnaR0g2yJfmg1NmK5F13~6TnrRLRcQOZeTx-ysu0SXJ8ZlXMCrYsK3W~PN9lx9pNL09S1bCFooGfubkYQAxcqRwu~2ITU~~Uq5pEikmjAVIKKKCp~dd9~26-ZC3AC3D9Ak-XVhVfjfMDtQCSdHpZJT46GceCX5tCNeXqVBoTVFQRe3f2QsU4F0YLRG-2AE9DLTxoIrheT7VYGeTE1dvf6uTmS8lYzK3me6zUwJXVeMpKfTPlicTyCEqB9M07rkpCI~h7mDpIaFvYNE3HfDG5lkhBW2B81XmCJsytZQm2lZx4Gh84g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680193"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680193/Uncovering_Deletion_Insertion_Mutations_in_Veno_Occlusive_Disease_with_Immunodeficiency_Syndrome_in_An_Iranian_Family_A_Case_Report"><img alt="Research paper thumbnail of Uncovering Deletion/Insertion Mutations in Veno-Occlusive Disease with Immunodeficiency Syndrome in An Iranian Family: A Case Report" class="work-thumbnail" src="https://attachments.academia-assets.com/121377766/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680193/Uncovering_Deletion_Insertion_Mutations_in_Veno_Occlusive_Disease_with_Immunodeficiency_Syndrome_in_An_Iranian_Family_A_Case_Report">Uncovering Deletion/Insertion Mutations in Veno-Occlusive Disease with Immunodeficiency Syndrome in An Iranian Family: A Case Report</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Veno-occlusive disease with immunodeficiency (VODI) syndrome is a rare genetic disorder character...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Veno-occlusive disease with immunodeficiency (VODI) syndrome is a rare genetic disorder characterized by immune system irregularities and a significant mortality rate, despite its infrequency. SP110, situated on chromosome 2q37.1, plays a pivotal role in VODI syndrome, and its association with tuberculosis has been extensively studied. The identification of SP110 mutations holds promise for accelerating the diagnosis and treatment of VODI syndrome, by providing a comprehensive panel for diagnosis and potentially leading to targeted therapies. In this case study, we examined a three-year-old girl born to a consanguineous union who was suspected of having an immunodeficiency disorder. Whole-exome sequencing (WES) and clinical assessments were conducted to screen for and confirm potentially pathogenic mutations. The detected mutation was further analyzed using bioinformatics tools to forecast its impact on protein structure. WES analysis revealed a novel deletion-insertion mutation, c.1181-1182delAGinsT, within SP110. Protein analysis indicated substantial structural modifications in the SP110 protein. This study identified a novel deletion-insertion mutation as a potential contributor to VODI syndrome by affecting the functionality of the SP110 protein. By including various mutations associated with the SP110 gene, this study aimed to expedite diagnosis by creating a comprehensive panel for VODI syndrome.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7553e60a8771bad8bf2e1355523957f1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377766,"asset_id":127680193,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377766/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680193"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680193"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680193; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680193]").text(description); $(".js-view-count[data-work-id=127680193]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680193; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680193']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7553e60a8771bad8bf2e1355523957f1" } } $('.js-work-strip[data-work-id=127680193]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680193,"title":"Uncovering Deletion/Insertion Mutations in Veno-Occlusive Disease with Immunodeficiency Syndrome in An Iranian Family: A Case Report","internal_url":"https://www.academia.edu/127680193/Uncovering_Deletion_Insertion_Mutations_in_Veno_Occlusive_Disease_with_Immunodeficiency_Syndrome_in_An_Iranian_Family_A_Case_Report","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377766,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377766/thumbnails/1.jpg","file_name":"Cell_J_26_515.pdf","download_url":"https://www.academia.edu/attachments/121377766/download_file","bulk_download_file_name":"Uncovering_Deletion_Insertion_Mutations.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377766/Cell_J_26_515-libre.pdf?1739687767=\u0026response-content-disposition=attachment%3B+filename%3DUncovering_Deletion_Insertion_Mutations.pdf\u0026Expires=1740213530\u0026Signature=OCpEYj-8SfKky4IQg~aPpMnfTA1dx6IbNZtLu-iv9qHE5CPWQZqMY6r7SJk71SUFlQxBa~49mnHzAak202YriqZj9OrGZrSootJgXtAUyMV8Dk9hVZa0SAIuNlTrzTEOAsRiF97X9po9eY8Xm3R3k1Fps1GrUYiU6iFYVIbSwA4Z980C6WDPNjsmInfv8UPyt05hSD2OCPwpLwJnud5vnE-oyxie12-hXvmd9H-DHkCBhcaf~UUQlBfbJ8~IlluRlmiLbYEv-k84mLCItSq9aFBsEnHbCubtQxfnv3x013Fg-3hkVKsM~Rw5gdzrg9C~IeNEmDmYlbhbQc4A-AM-9Q__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680183"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680183/Application_of_XBP1s_Decoy_Oligodeoxynucleotide_Attenuates_Cancerous_Phenotype_in_Huh_7_Hepatocellular_Carcinoma_Cells"><img alt="Research paper thumbnail of Application of XBP1s Decoy Oligodeoxynucleotide Attenuates Cancerous Phenotype in Huh-7 Hepatocellular Carcinoma Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/121377711/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680183/Application_of_XBP1s_Decoy_Oligodeoxynucleotide_Attenuates_Cancerous_Phenotype_in_Huh_7_Hepatocellular_Carcinoma_Cells">Application of XBP1s Decoy Oligodeoxynucleotide Attenuates Cancerous Phenotype in Huh-7 Hepatocellular Carcinoma Cells</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: The spliced form of X-box binding protein 1 (XBP1s) is a key transcription factor in t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: The spliced form of X-box binding protein 1 (XBP1s) is a key transcription factor in the unfolded protein response (UPR), an adaptive mechanism for cell survival. Many studies demonstrated the induced expression of XBP1s in various cancers, including hepatocellular carcinoma (HCC). Such upregulated expression is linked to an enhancement of cell proliferation, migration, and improvement of the survival rate. In this study, we aimed to assess the therapeutic potential of targeting XBP1s, by specific decoy oligodeoxynucleotide (ODN) and evaluated the cancerous phenotypes in Huh-7 cells. Materials and Methods: In this experimental study, we transfected Huh-7 cells with XBP1s decoy oligonucleotide (ODN). Subsequently, we assess some cellular features, including viability, migration capacity, proliferation potential, and apoptosis. Therefore, various techniques included wound healing test, BrdU, and annexin/PI assays. Additionally, the colony formation capacity was evaluated. The mRNA expression levels of BAX, BCL-2, c-MYC, CCND1, MMP-9, CDH1, and CD133 were quantified by the reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results: Transfection of Huh-7 cells by XBP1s decoy ODN led to significant down-regulation of c-Myc, CCND1, MMP-9, BCL-2 and CD133 and up-regulation of CDH1 and BAX transcriptional expressions in comparison with the vehicle group. Our results also demonstrated that transfection of XBP1s-decoy reduced HCC cell viability, proliferation, migration capacity as well as colonization ability in comparison with the vehicle group. Conclusion: These findings proposed the potential application of XBP1s-decoy ODN to reduce cancerous phenotypes such as cell proliferation, cell migration and apoptosis induction in the Huh-7 cell line. More experiments on other cell lines and primary cells could validate our results.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1a4e524033f69acbe2c47dfc0498f146" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377711,"asset_id":127680183,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377711/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680183"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680183"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680183; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680183]").text(description); $(".js-view-count[data-work-id=127680183]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680183; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680183']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1a4e524033f69acbe2c47dfc0498f146" } } $('.js-work-strip[data-work-id=127680183]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127680183,"title":"Application of XBP1s Decoy Oligodeoxynucleotide Attenuates Cancerous Phenotype in Huh-7 Hepatocellular Carcinoma Cells","internal_url":"https://www.academia.edu/127680183/Application_of_XBP1s_Decoy_Oligodeoxynucleotide_Attenuates_Cancerous_Phenotype_in_Huh_7_Hepatocellular_Carcinoma_Cells","owner_id":126987384,"coauthors_can_edit":true,"owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":121377711,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121377711/thumbnails/1.jpg","file_name":"Cell_J_26_505.pdf","download_url":"https://www.academia.edu/attachments/121377711/download_file","bulk_download_file_name":"Application_of_XBP1s_Decoy_Oligodeoxynuc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121377711/Cell_J_26_505.pdf?1739686081=\u0026response-content-disposition=attachment%3B+filename%3DApplication_of_XBP1s_Decoy_Oligodeoxynuc.pdf\u0026Expires=1739691332\u0026Signature=OpSe46~jFRB2EsVIwQuS9EzNZms~Ved9B6Ir~ptbanGk7LYIvzGgHbY0Kq~ZTxVMJX4E3zu6HloqjnVoVXqBVFlkSZhFfXeUqgjY3OyZk0eNoFQcODqoKUO3lK9ap3TV33GQyGEAEsArEt84zllYW2keiCdOjmMJ6eJ4x2vkMTAHn~FXBTM2Gp-WGUl5YiEbRnVztGRwitVIB40BuBGEz-7DSoZiXdiynj4OeM6mAnBdLWpIH4vOGrcMgaWr1QNKxSwfL5NFk-DQWowrpNNNMHFT3RXI6mi9jildYLT0kt0iKxCrCKBxANrhbiKpDnrsrfvg1REcDEyS6immYH2~Gw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680072"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680072/Poncirin_Impact_on_Human_HER2_Breast_Cancer_Cells_Inhibiting_Proliferation_Metastasis_and_Tumor_Growth_in_Mice_Potentially_through_The_PI3K_AKT_Pathway"><img alt="Research paper thumbnail of Poncirin Impact on Human HER2 Breast Cancer Cells: Inhibiting Proliferation, Metastasis, and Tumor Growth in Mice Potentially through The PI3K/AKT Pathway" class="work-thumbnail" src="https://attachments.academia-assets.com/121377660/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680072/Poncirin_Impact_on_Human_HER2_Breast_Cancer_Cells_Inhibiting_Proliferation_Metastasis_and_Tumor_Growth_in_Mice_Potentially_through_The_PI3K_AKT_Pathway">Poncirin Impact on Human HER2 Breast Cancer Cells: Inhibiting Proliferation, Metastasis, and Tumor Growth in Mice Potentially through The PI3K/AKT Pathway</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Breast cancer is a prevalent and heterogeneous disease, with human epidermal growth fa...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Breast cancer is a prevalent and heterogeneous disease, with human epidermal growth factor receptor-2 (HER2) overexpression occurring in over 20% of cases. Poncirin, a biologically active flavonone derived from the immature dried fruits of Poncirus trifoliata, is a 7-O-neohesperidoside of isosakuranetin with a well-documented history in traditional Chinese medicine for its health-promoting properties. While the previous research hinted at its potential as an anticancer agent, its specific effects on HER2 overexpressing breast cancer cells remain largely unexplored. The aim of this study is to investigate the specific effects of Poncirin, on HER2 overexpressing breast cancer cells. Materials and Methods: In experimental study, we assessed cell proliferation using the CCK-8 assay and explored cell migration and invasion with transwell assays. Additionally, we evaluated colony formation ability and examined apoptosis through the acridine orange/ethidium bromide (AO/EB) and Annexin V-fluorescein isothiocyanate (FITC)/ propidium iodide (PI) staining methods. The study also delved into the molecular mechanisms involved by scrutinizing the phosphatidylinositol 3-kinase/serine-threonine protein kinase (PI3K/AKT) signaling pathway via Western blotting. Furthermore, the researchers conducted in vivo experiments using mouse models to corroborate the findings in a living organism. Results: Poncirin demonstrated a remarkable ability to selectively inhibit proliferation and metastasis of HER2 overexpressing breast cancer cells. Mechanistically, the compound seemed to exert its effects by modulating the PI3K/AKT signaling pathway, implying its central role in the observed anticancer effects. These findings were further substantiated by in vivo experiments, which consistently showed a reduction in tumor growth when poncirin was administered. Conclusion: This study underscores potential of poncirin as a potent agent for restraining the growth and metastasis of HER2 overexpressing breast cancer cells. The evidence suggests that poncirin efficacy may be attributed to its modulation possibly through PI3K/AKT pathway.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c08db02e4f05546d81598248daa7ff58" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377660,"asset_id":127680072,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377660/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680072"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680072"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680072; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127680072]").text(description); $(".js-view-count[data-work-id=127680072]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127680072; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127680072']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680030"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680030/Development_and_Characterization_of_A_Novel_SpyTagged_Modular_Nanobody_as_A_Detection_Platform_for_CD22_Positive_Cells"><img alt="Research paper thumbnail of Development and Characterization of A Novel SpyTagged Modular Nanobody as A Detection Platform for CD22-Positive Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/121377646/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680030/Development_and_Characterization_of_A_Novel_SpyTagged_Modular_Nanobody_as_A_Detection_Platform_for_CD22_Positive_Cells">Development and Characterization of A Novel SpyTagged Modular Nanobody as A Detection Platform for CD22-Positive Cells</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: CD22, as a surface protein of B cells, is used in the diagnosis and target-specific im...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: CD22, as a surface protein of B cells, is used in the diagnosis and target-specific immunotherapy of B-cell malignancies. SpyTag and SpyCatcher, on the other hand, are two covalently coupled proteins capable of developing a bi-or multi-specific modular protein. The aim of this study was to develop FITC-conjugated SpyCatcher-SpyTagged anti-CD22 Nanobody (FITC-SpyC-SpyT-CD22Nb) to recognize CD22 on the surface of malignant B cells. Materials and Methods: In this experimental study, the SpyTag-CD22Nb construct was subcloned into a pET22 vector and expressed in E. coli BL21 (DE3). After purification using His-tag affinity chromatography, the size of the eluted protein was confirmed on a Western blot. In addition, the SpyCatcher protein, subcloned into pET28, was expressed in E. coli BL21 (DE3), purified by His-tag affinity chromatography and subjected to FITC labeling. FITC-SpyCatcher and SpyTag-CD22Nb were coupled in a 1:1 molar ratio. The specific binding of the produced FITC-SpyC-SpyT-CD22Nb was tested using CD22-positive Raji and CD22-negative K562 cell lines and was evaluated by flow cytometry Results: SpyTag-CD22Nb and SpyCatcher were successfully expressed in E. coli BL21 (DE3). The 1:1 molar ratio of SpyTag-CD22Nb and FITC-SpyCatcher successfully formed FITC-SpyC-SpyT-CD22Nb at room temperature. The flow cytometry results showed that FITC-SpyC-SpyT-CD22Nb specifically binds to the CD22-positive Raji cells, while there is no binding to the CD22-negative K562 control cells. Conclusion: The novel FITC-SpyC-SpyT-CD22Nb produced in the present study is capable of detecting the surficial expression of CD22. According to our findings, FITC-SpyC-SpyT-CD22Nb is applicable for specific targeting of CD22 in a therapeutic manner, i.e., chimeric antigen receptor (CAR)-T cell therapy and antibody drug conjugates (ADCs).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3f98002817668a4f063e5bc8d89b3ae9" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377646,"asset_id":127680030,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377646/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680030"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680030"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680030; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="127680021"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127680021/Therapeutic_Effects_of_Althaea_officinalis_L_and_Metformin_on_Estradiol_Induced_Polycystic_Ovary_Syndrome_in_Rats_Insights_into_The_PI3K_AKT_Pathway_Inflammation_and_Oxidative_Stress"><img alt="Research paper thumbnail of Therapeutic Effects of Althaea officinalis L. and Metformin on Estradiol-Induced Polycystic Ovary Syndrome in Rats: Insights into The PI3K/AKT Pathway, Inflammation, and Oxidative Stress" class="work-thumbnail" src="https://attachments.academia-assets.com/121377640/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127680021/Therapeutic_Effects_of_Althaea_officinalis_L_and_Metformin_on_Estradiol_Induced_Polycystic_Ovary_Syndrome_in_Rats_Insights_into_The_PI3K_AKT_Pathway_Inflammation_and_Oxidative_Stress">Therapeutic Effects of Althaea officinalis L. and Metformin on Estradiol-Induced Polycystic Ovary Syndrome in Rats: Insights into The PI3K/AKT Pathway, Inflammation, and Oxidative Stress</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 8, August </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Polycystic ovary syndrome (PCOS) is one of the most important causes of infertility, i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Polycystic ovary syndrome (PCOS) is one of the most important causes of infertility, irregular menstrual cycles, and anovulation in women. The current study aimed to investigate the therapeutic effects of Althaea officinalis L. (A. officinale) extract on PCOS in rats. Materials and Methods: In this experimental study, 70 rats in 7 groups (n=10/group) were studied for three weeks as follows; healthy control (HC), patient (PCOS), metformin (PCOS+MET), A. officinale treatment (PCOS+250 and 500 mg/kg A. officinale) and synergistic (PCOS+MET+250 and 500 mg/kg A. officinale) groups. Luteinizing hormone (LH), folliclestimulating hormone (FSH), progesterone (P) and testosterone (T) levels as well as inflammatory cytokines were measured. Total antioxidant capacity and lipid peroxidation levels were analyzed in ovarian tissue. The expression of GLUT-4, AKT, PI3K, PTEN genes and Ki-67 was assessed by real-time polymerase chain reaction (PCR) and immunohistochemistry, respectively. Results: A. officinale alone and especially in combination with MET moderated inflammatory and antioxidant parameters compared to the PCOS and MET groups. A. officinale in synergistic groups increased the apoptosis of granulosa cells by activating the PI3K/AKT pathway, resulting in a rise in the number of Ki-67 positive cells (P<0.05). Furthermore, following A. officinale treatment the LH/FSH rate decreased and FSH and P increased (P<0.05). Also, A. officinale extract could effectively normalize estrus cycle duration close to the normal group. Conclusion: The extract of A. officinale, in combination with metformin, can enhance the hypothalamic-pituitaryovary (HPO) axis with synergistic anti-inflammatory and antioxidant effects. Additionally, the extract showed apoptotic effect on cystic granulosa cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e7217f6aee84f6dab65a1322d45e95a3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121377640,"asset_id":127680021,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121377640/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127680021"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127680021"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127680021; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125098009"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125098009/High_Intensity_Interval_Training_and_Vitamin_D3_Supplementation_Decrease_CCL_5_and_CCR5_Expression_In_White_Adipose_Tissue_of_Diabetic_Rats_Fed_with_A_High_Fat_Diet_and_Streptozotocin"><img alt="Research paper thumbnail of High-Intensity Interval Training and Vitamin D3 Supplementation Decrease CCL-5 and CCR5 Expression In White Adipose Tissue of Diabetic Rats Fed with A High-Fat Diet and Streptozotocin" class="work-thumbnail" src="https://attachments.academia-assets.com/119202176/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125098009/High_Intensity_Interval_Training_and_Vitamin_D3_Supplementation_Decrease_CCL_5_and_CCR5_Expression_In_White_Adipose_Tissue_of_Diabetic_Rats_Fed_with_A_High_Fat_Diet_and_Streptozotocin">High-Intensity Interval Training and Vitamin D3 Supplementation Decrease CCL-5 and CCR5 Expression In White Adipose Tissue of Diabetic Rats Fed with A High-Fat Diet and Streptozotocin</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and CC motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM). Materials and Methods: The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks. Results: The levels of CCL-5 (P<0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P<0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P< 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P<0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05). Conclusion: The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ee39a96f203a356e5a289dd5466b4beb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119202176,"asset_id":125098009,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119202176/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125098009"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125098009"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125098009; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125098009]").text(description); $(".js-view-count[data-work-id=125098009]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125098009; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125098009']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ee39a96f203a356e5a289dd5466b4beb" } } $('.js-work-strip[data-work-id=125098009]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125098009,"title":"High-Intensity Interval Training and Vitamin D3 Supplementation Decrease CCL-5 and CCR5 Expression In White Adipose Tissue of Diabetic Rats Fed with A High-Fat Diet and Streptozotocin","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2027582.1560","abstract":"Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and CC motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM). Materials and Methods: The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks. Results: The levels of CCL-5 (P\u003c0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P\u003c0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P\u003c 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P\u003c0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05). Conclusion: The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7"},"translated_abstract":"Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and CC motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM). Materials and Methods: The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks. Results: The levels of CCL-5 (P\u003c0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P\u003c0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P\u003c 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P\u003c0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05). Conclusion: The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.","internal_url":"https://www.academia.edu/125098009/High_Intensity_Interval_Training_and_Vitamin_D3_Supplementation_Decrease_CCL_5_and_CCR5_Expression_In_White_Adipose_Tissue_of_Diabetic_Rats_Fed_with_A_High_Fat_Diet_and_Streptozotocin","translated_internal_url":"","created_at":"2024-10-28T00:35:57.834-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615489,"work_id":125098009,"tagging_user_id":126987384,"tagged_user_id":294848381,"co_author_invite_id":null,"email":"w***i@razi.ac.ir","affiliation":"Razi University of Kermanshah, Iran","display_order":1,"name":"Worya Tahmasebi","title":"High-Intensity Interval Training and Vitamin D3 Supplementation Decrease CCL-5 and CCR5 Expression In White Adipose Tissue of Diabetic Rats Fed with A High-Fat Diet and Streptozotocin"}],"downloadable_attachments":[{"id":119202176,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119202176/thumbnails/1.jpg","file_name":"Cell_J_26_465.pdf","download_url":"https://www.academia.edu/attachments/119202176/download_file","bulk_download_file_name":"High_Intensity_Interval_Training_and_Vit.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119202176/Cell_J_26_465-libre.pdf?1730101696=\u0026response-content-disposition=attachment%3B+filename%3DHigh_Intensity_Interval_Training_and_Vit.pdf\u0026Expires=1738763676\u0026Signature=KETei855gHtcs2HGgcrVMrQgT1sMt1wtKvBL4VUH-mC3kBi4cDHvtwGAsWu0KxdshInE0zJaFCgYT-1JVgnXRdpkqfAvPTSX37l8RBuRg3WOW~Uc-OmJAmWgSZC7EM08Ryuwh~B1EQKsmFOumjz7gnJNUvYy6iwthFAAIC23FjRgsZXv-f43RfoBhwyhqUmz8ccwKje6i368z7wyly9moGGr~lFHpAEnQOVU9HsPTEyE4hAYI5TAEALFKqtLwUYZ2XLwIM5ErTWBtm8-bjPoSaEz4iV1UvYEMvQFEtheDHYayk5OJjIiBqOhvWkpAAbvwoYI~EyI7RcbJ-GqBbV6jA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"High_Intensity_Interval_Training_and_Vitamin_D3_Supplementation_Decrease_CCL_5_and_CCR5_Expression_In_White_Adipose_Tissue_of_Diabetic_Rats_Fed_with_A_High_Fat_Diet_and_Streptozotocin","translated_slug":"","page_count":8,"language":"en","content_type":"Work","summary":"Objective: The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and CC motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM). Materials and Methods: The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks. Results: The levels of CCL-5 (P\u003c0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P\u003c0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P\u003c 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P\u003c0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05). Conclusion: The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119202176,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119202176/thumbnails/1.jpg","file_name":"Cell_J_26_465.pdf","download_url":"https://www.academia.edu/attachments/119202176/download_file","bulk_download_file_name":"High_Intensity_Interval_Training_and_Vit.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119202176/Cell_J_26_465-libre.pdf?1730101696=\u0026response-content-disposition=attachment%3B+filename%3DHigh_Intensity_Interval_Training_and_Vit.pdf\u0026Expires=1738763676\u0026Signature=KETei855gHtcs2HGgcrVMrQgT1sMt1wtKvBL4VUH-mC3kBi4cDHvtwGAsWu0KxdshInE0zJaFCgYT-1JVgnXRdpkqfAvPTSX37l8RBuRg3WOW~Uc-OmJAmWgSZC7EM08Ryuwh~B1EQKsmFOumjz7gnJNUvYy6iwthFAAIC23FjRgsZXv-f43RfoBhwyhqUmz8ccwKje6i368z7wyly9moGGr~lFHpAEnQOVU9HsPTEyE4hAYI5TAEALFKqtLwUYZ2XLwIM5ErTWBtm8-bjPoSaEz4iV1UvYEMvQFEtheDHYayk5OJjIiBqOhvWkpAAbvwoYI~EyI7RcbJ-GqBbV6jA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1383570,"name":"Vitamin D3","url":"https://www.academia.edu/Documents/in/Vitamin_D3"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097935"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097935/Mac_1_Alongside_Platelet_Monocyte_Aggregates_as_Potential_Markers_in_Acute_Coronary_Syndrome_A_Case_Control_Study"><img alt="Research paper thumbnail of Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study" class="work-thumbnail" src="https://attachments.academia-assets.com/119201977/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097935/Mac_1_Alongside_Platelet_Monocyte_Aggregates_as_Potential_Markers_in_Acute_Coronary_Syndrome_A_Case_Control_Study">Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://royaninstitute.academia.edu/CellJournalYakhteh">Cell Journal Yakhteh</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MohsenHamidpour1">Mohsen Hamidpour</a></span></div><div class="wp-workCard_item"><span>Volume 26, Issue 7 </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosc...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P<0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11b expression on monocytes were significantly elevated in patients (P<0.001) and were positively associated with PMA (β=19.09, P<0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06, P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals. (respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70). Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally, the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationship between these markers and ACS.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6755544423b4829d290af27b6c47753f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201977,"asset_id":125097935,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201977/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097935"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097935"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097935; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097935]").text(description); $(".js-view-count[data-work-id=125097935]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097935; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097935']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6755544423b4829d290af27b6c47753f" } } $('.js-work-strip[data-work-id=125097935]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097935,"title":"Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2024525.1527","abstract":"Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P\u003c0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11b expression on monocytes were significantly elevated in patients (P\u003c0.001) and were positively associated with PMA (β=19.09, P\u003c0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06, P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals. (respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70). Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally, the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationship between these markers and ACS.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7 "},"translated_abstract":"Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P\u003c0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11b expression on monocytes were significantly elevated in patients (P\u003c0.001) and were positively associated with PMA (β=19.09, P\u003c0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06, P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals. (respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70). Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally, the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationship between these markers and ACS.","internal_url":"https://www.academia.edu/125097935/Mac_1_Alongside_Platelet_Monocyte_Aggregates_as_Potential_Markers_in_Acute_Coronary_Syndrome_A_Case_Control_Study","translated_internal_url":"","created_at":"2024-10-28T00:33:35.072-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615480,"work_id":125097935,"tagging_user_id":126987384,"tagged_user_id":330410199,"co_author_invite_id":8272079,"email":"m***p@sbmu.ac.ir","display_order":1,"name":"Mohsen Hamidpour","title":"Mac-1 Alongside Platelet-Monocyte Aggregates as Potential Markers in Acute Coronary Syndrome: A Case-Control Study"}],"downloadable_attachments":[{"id":119201977,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201977/thumbnails/1.jpg","file_name":"Cell_J_26_454.pdf","download_url":"https://www.academia.edu/attachments/119201977/download_file","bulk_download_file_name":"Mac_1_Alongside_Platelet_Monocyte_Aggreg.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201977/Cell_J_26_454-libre.pdf?1730101724=\u0026response-content-disposition=attachment%3B+filename%3DMac_1_Alongside_Platelet_Monocyte_Aggreg.pdf\u0026Expires=1738763676\u0026Signature=PopnZLREOsE2sBOxslo05GAND5LQ7eXZVsxG3N-YEkHP5Au7Uv0jpNwk2xkyaaLehMP6u6E8niPs6Q4u9K4BnozFneNrCYXr3kxRh2w~94wFGMSbkcc4ikeaONr44qmzxnJdfJmMhnsoDYQ7OWQ2LOZqPJu2pnOFRjWtkWtU7biJPUGMkGBd76RntEl6U3Bm4X-6NLJI~A821JcZRkYT4H2DTj1uClY9iNKf-HcaGw7cXX1n-UN5oG2PW-PkJifJVtaVVcObfSoowyYzwXqPUJjZK6-AynllT5LxEdk22Vm71b0xEVoRi3HD2v9a9Q7y3wqSBuJlAJ~fcob~7wE-iA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Mac_1_Alongside_Platelet_Monocyte_Aggregates_as_Potential_Markers_in_Acute_Coronary_Syndrome_A_Case_Control_Study","translated_slug":"","page_count":11,"language":"en","content_type":"Work","summary":"Objective: Cardiovascular diseases (CVDs) are the leading cause of death worldwide, with atherosclerosis serving as a primary factor in their development. Platelets, leukocytes, and their interactions play a crucial role in initiating and amplifying atherosclerosis. This study aims to evaluate the levels of platelet-monocyte aggregates (PMA) and specific integrins involved in leukocyte recruitment, including macrophage-1 antigen (Mac-1) and lymphocyte functionassociated antigen-1 (Lfa-1), in patients with acute coronary syndrome (ACS). Materials and Methods: In this case-control study, thirty-two subjects with ACS and 30 healthy individuals participated. It aimed to evaluate PMA expression and the median fluorescence intensity (MFI) of Mac-1 and Lfa-1 using flow cytometry. Dot plots and Pearson correlation coefficient were employed to examine the relationship between PMA, Mac-1, and Lfa-1. Multilevel model analysis was used to explore the effects and relationships of various parameters, including Mac-1 and Lfa-1, on PMA. Finally, receiver operating characteristic (ROC) curves were utilized to assess the diagnostic accuracy of PMA, Mac-1, and Lfa-1 markers. Results: It was observed that patients had higher PMA levels compared to the control group (58.99 ± 16.27 vs. 29.99 ± 4.19 in controls, P\u003c0.001), which correlated with PLT (ρ=0.512, P=0.035). Additionally, CD18 and CD11b expression on monocytes were significantly elevated in patients (P\u003c0.001) and were positively associated with PMA (β=19.09, P\u003c0.001; β=6.90, P=0.022), but no significant relationship between CD11a and PMA was observed (β=5.06, P=0.315). PMA and Mac-1 were identified as better markers for differentiating patients from healthy individuals. (respectively, AUC=0.94, Sensitivity= 0.84, specificity=0.98; AUC=0.84, Sensitivity= 0.93, specificity=0.70). Conclusion: The study results indicated an increase in both Mac-1 and PMA levels in patients with ACS. Additionally, the significant association observed between Mac-1 and PMA in the patient group suggests a potential relationship between these markers and ACS.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201977,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201977/thumbnails/1.jpg","file_name":"Cell_J_26_454.pdf","download_url":"https://www.academia.edu/attachments/119201977/download_file","bulk_download_file_name":"Mac_1_Alongside_Platelet_Monocyte_Aggreg.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201977/Cell_J_26_454-libre.pdf?1730101724=\u0026response-content-disposition=attachment%3B+filename%3DMac_1_Alongside_Platelet_Monocyte_Aggreg.pdf\u0026Expires=1738763676\u0026Signature=PopnZLREOsE2sBOxslo05GAND5LQ7eXZVsxG3N-YEkHP5Au7Uv0jpNwk2xkyaaLehMP6u6E8niPs6Q4u9K4BnozFneNrCYXr3kxRh2w~94wFGMSbkcc4ikeaONr44qmzxnJdfJmMhnsoDYQ7OWQ2LOZqPJu2pnOFRjWtkWtU7biJPUGMkGBd76RntEl6U3Bm4X-6NLJI~A821JcZRkYT4H2DTj1uClY9iNKf-HcaGw7cXX1n-UN5oG2PW-PkJifJVtaVVcObfSoowyYzwXqPUJjZK6-AynllT5LxEdk22Vm71b0xEVoRi3HD2v9a9Q7y3wqSBuJlAJ~fcob~7wE-iA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097570"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097570/Local_Transplantation_of_Mesenchymal_Stromal_Cells_Is_Safe_and_Could_Alleviate_Kienb%C3%B6ck_Diseases_Complications_A_Clinical_Trial_Study"><img alt="Research paper thumbnail of Local Transplantation of Mesenchymal Stromal Cells Is Safe and Could Alleviate Kienböck Disease's Complications: A Clinical Trial Study" class="work-thumbnail" src="https://attachments.academia-assets.com/119201821/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097570/Local_Transplantation_of_Mesenchymal_Stromal_Cells_Is_Safe_and_Could_Alleviate_Kienb%C3%B6ck_Diseases_Complications_A_Clinical_Trial_Study">Local Transplantation of Mesenchymal Stromal Cells Is Safe and Could Alleviate Kienböck Disease's Complications: A Clinical Trial Study</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist movement. Various palliative methods have been proposed as therapeutic strategies for alleviating symptoms. Mesenchymal stromal cell transplantation has been suggested as an innovative and promising approach due to its potential for inducing regeneration and immunomodulation in the necrotic tissue. This study aims to evaluate the safety of autologous bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation after core decompression in Kienböck disease. Materials and Methods: In this phase I of an open-label clinical trial, three patients (one female and two males) with stage 2 Kienböck disease underwent autologous BM-MSCs transplantation following lunate core decompression. The patients were followed up for six months to assess safety as well as secondary clinical outcomes, including pain level, range of motion (ROM), and functional disability. Results: Safety of BM-MSCs injection following the core decompression was evaluated by recording post-treatment complications during the six-month follow-up. No adverse events (AEs) or severe AEs (SAEs) were reported, indicating that BM-MSCs injection after core decompression is a safe intervention. All patients showed a remarkable reduction in visual analog scale (VAS) scores and "Disabilities of the Arm, Shoulder, and Hand" (DASH) questionnaire scores, suggesting the therapeutic potential of this intervention. Moreover, an increase in the ROM indicated that BM-MSCs transplantation can improve wrist functionality. Additionally, radiographic assessments before and after cell infusion demonstrated a reduction in lunate sclerosis after six months of follow-up. Conclusion: The transplantation of autologous BM-MSCs following lunate core decompression seems to be a safe clinical intervention and may lead to pain relief in patients with Kienböck disease. Furthermore, this procedure may help prevent disease progression during the follow-up period (registration number: NCT02646007).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="677cf4d0d2bba32f088c4764f89a7cd0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201821,"asset_id":125097570,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201821/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097570"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097570"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097570; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097570]").text(description); $(".js-view-count[data-work-id=125097570]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097570; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097570']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "677cf4d0d2bba32f088c4764f89a7cd0" } } $('.js-work-strip[data-work-id=125097570]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097570,"title":"Local Transplantation of Mesenchymal Stromal Cells Is Safe and Could Alleviate Kienböck Disease's Complications: A Clinical Trial Study","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2028891.1572","abstract":"Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist movement. Various palliative methods have been proposed as therapeutic strategies for alleviating symptoms. Mesenchymal stromal cell transplantation has been suggested as an innovative and promising approach due to its potential for inducing regeneration and immunomodulation in the necrotic tissue. This study aims to evaluate the safety of autologous bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation after core decompression in Kienböck disease. Materials and Methods: In this phase I of an open-label clinical trial, three patients (one female and two males) with stage 2 Kienböck disease underwent autologous BM-MSCs transplantation following lunate core decompression. The patients were followed up for six months to assess safety as well as secondary clinical outcomes, including pain level, range of motion (ROM), and functional disability. Results: Safety of BM-MSCs injection following the core decompression was evaluated by recording post-treatment complications during the six-month follow-up. No adverse events (AEs) or severe AEs (SAEs) were reported, indicating that BM-MSCs injection after core decompression is a safe intervention. All patients showed a remarkable reduction in visual analog scale (VAS) scores and \"Disabilities of the Arm, Shoulder, and Hand\" (DASH) questionnaire scores, suggesting the therapeutic potential of this intervention. Moreover, an increase in the ROM indicated that BM-MSCs transplantation can improve wrist functionality. Additionally, radiographic assessments before and after cell infusion demonstrated a reduction in lunate sclerosis after six months of follow-up. Conclusion: The transplantation of autologous BM-MSCs following lunate core decompression seems to be a safe clinical intervention and may lead to pain relief in patients with Kienböck disease. Furthermore, this procedure may help prevent disease progression during the follow-up period (registration number: NCT02646007).","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7"},"translated_abstract":"Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist movement. Various palliative methods have been proposed as therapeutic strategies for alleviating symptoms. Mesenchymal stromal cell transplantation has been suggested as an innovative and promising approach due to its potential for inducing regeneration and immunomodulation in the necrotic tissue. This study aims to evaluate the safety of autologous bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation after core decompression in Kienböck disease. Materials and Methods: In this phase I of an open-label clinical trial, three patients (one female and two males) with stage 2 Kienböck disease underwent autologous BM-MSCs transplantation following lunate core decompression. The patients were followed up for six months to assess safety as well as secondary clinical outcomes, including pain level, range of motion (ROM), and functional disability. Results: Safety of BM-MSCs injection following the core decompression was evaluated by recording post-treatment complications during the six-month follow-up. No adverse events (AEs) or severe AEs (SAEs) were reported, indicating that BM-MSCs injection after core decompression is a safe intervention. All patients showed a remarkable reduction in visual analog scale (VAS) scores and \"Disabilities of the Arm, Shoulder, and Hand\" (DASH) questionnaire scores, suggesting the therapeutic potential of this intervention. Moreover, an increase in the ROM indicated that BM-MSCs transplantation can improve wrist functionality. Additionally, radiographic assessments before and after cell infusion demonstrated a reduction in lunate sclerosis after six months of follow-up. Conclusion: The transplantation of autologous BM-MSCs following lunate core decompression seems to be a safe clinical intervention and may lead to pain relief in patients with Kienböck disease. Furthermore, this procedure may help prevent disease progression during the follow-up period (registration number: NCT02646007).","internal_url":"https://www.academia.edu/125097570/Local_Transplantation_of_Mesenchymal_Stromal_Cells_Is_Safe_and_Could_Alleviate_Kienb%C3%B6ck_Diseases_Complications_A_Clinical_Trial_Study","translated_internal_url":"","created_at":"2024-10-28T00:21:45.805-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615439,"work_id":125097570,"tagging_user_id":126987384,"tagged_user_id":63914468,"co_author_invite_id":null,"email":"m***i@gmail.com","display_order":1,"name":"Marzieh ebrahimi","title":"Local Transplantation of Mesenchymal Stromal Cells Is Safe and Could Alleviate Kienböck Disease's Complications: A Clinical Trial Study"}],"downloadable_attachments":[{"id":119201821,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201821/thumbnails/1.jpg","file_name":"Cell_J_26_446.pdf","download_url":"https://www.academia.edu/attachments/119201821/download_file","bulk_download_file_name":"Local_Transplantation_of_Mesenchymal_Str.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201821/Cell_J_26_446-libre.pdf?1730101749=\u0026response-content-disposition=attachment%3B+filename%3DLocal_Transplantation_of_Mesenchymal_Str.pdf\u0026Expires=1738763676\u0026Signature=K312e4vmydI9Moa6~p6awxflsiKsuA0pCX2FLC7AnA2V~NCnnfgJOFb1k2mWrwvIgRgolCQEP5AJF2d9uwIQUpD9yMoLytrUEE8hUvF82GySKzOD6dRVA3mDGqJ3EDMg8n~ohP7USnTrFCus4Kw4UsvHZLiMGRsLd3E9zzGdYKFqmC8BzDgl4xWnG3ckUJddrNcTtIOazxYql1~Izp7fdEZ-iErtooccHcZGPfU8ngGT-e4mO9KJ2Ivy8F023hLCuz7M8~IiOtXVuy~I8OTpdC2AxgS88PpQvQBpNNGCLy~p0o2gtVxfHuUh65MVDge1YCbCysZ~O5F0Xe8HVM3ZFA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Local_Transplantation_of_Mesenchymal_Stromal_Cells_Is_Safe_and_Could_Alleviate_Kienböck_Diseases_Complications_A_Clinical_Trial_Study","translated_slug":"","page_count":8,"language":"en","content_type":"Work","summary":"Objective: Kienböck disease is a rare condition characterized by severe pain and restricted wrist movement. Various palliative methods have been proposed as therapeutic strategies for alleviating symptoms. Mesenchymal stromal cell transplantation has been suggested as an innovative and promising approach due to its potential for inducing regeneration and immunomodulation in the necrotic tissue. This study aims to evaluate the safety of autologous bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation after core decompression in Kienböck disease. Materials and Methods: In this phase I of an open-label clinical trial, three patients (one female and two males) with stage 2 Kienböck disease underwent autologous BM-MSCs transplantation following lunate core decompression. The patients were followed up for six months to assess safety as well as secondary clinical outcomes, including pain level, range of motion (ROM), and functional disability. Results: Safety of BM-MSCs injection following the core decompression was evaluated by recording post-treatment complications during the six-month follow-up. No adverse events (AEs) or severe AEs (SAEs) were reported, indicating that BM-MSCs injection after core decompression is a safe intervention. All patients showed a remarkable reduction in visual analog scale (VAS) scores and \"Disabilities of the Arm, Shoulder, and Hand\" (DASH) questionnaire scores, suggesting the therapeutic potential of this intervention. Moreover, an increase in the ROM indicated that BM-MSCs transplantation can improve wrist functionality. Additionally, radiographic assessments before and after cell infusion demonstrated a reduction in lunate sclerosis after six months of follow-up. Conclusion: The transplantation of autologous BM-MSCs following lunate core decompression seems to be a safe clinical intervention and may lead to pain relief in patients with Kienböck disease. Furthermore, this procedure may help prevent disease progression during the follow-up period (registration number: NCT02646007).","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201821,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201821/thumbnails/1.jpg","file_name":"Cell_J_26_446.pdf","download_url":"https://www.academia.edu/attachments/119201821/download_file","bulk_download_file_name":"Local_Transplantation_of_Mesenchymal_Str.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201821/Cell_J_26_446-libre.pdf?1730101749=\u0026response-content-disposition=attachment%3B+filename%3DLocal_Transplantation_of_Mesenchymal_Str.pdf\u0026Expires=1738763676\u0026Signature=K312e4vmydI9Moa6~p6awxflsiKsuA0pCX2FLC7AnA2V~NCnnfgJOFb1k2mWrwvIgRgolCQEP5AJF2d9uwIQUpD9yMoLytrUEE8hUvF82GySKzOD6dRVA3mDGqJ3EDMg8n~ohP7USnTrFCus4Kw4UsvHZLiMGRsLd3E9zzGdYKFqmC8BzDgl4xWnG3ckUJddrNcTtIOazxYql1~Izp7fdEZ-iErtooccHcZGPfU8ngGT-e4mO9KJ2Ivy8F023hLCuz7M8~IiOtXVuy~I8OTpdC2AxgS88PpQvQBpNNGCLy~p0o2gtVxfHuUh65MVDge1YCbCysZ~O5F0Xe8HVM3ZFA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3176577,"name":"Avascular Necrosis","url":"https://www.academia.edu/Documents/in/Avascular_Necrosis"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097338"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097338/Potential_Advantages_of_Idarubicin_Loaded_Trastuzumab_Coated_Liposomes_for_Combating_Head_and_Neck_Squamous_Cancer_Cells"><img alt="Research paper thumbnail of Potential Advantages of Idarubicin-Loaded Trastuzumab-Coated Liposomes for Combating Head and Neck Squamous Cancer Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/119201626/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097338/Potential_Advantages_of_Idarubicin_Loaded_Trastuzumab_Coated_Liposomes_for_Combating_Head_and_Neck_Squamous_Cancer_Cells">Potential Advantages of Idarubicin-Loaded Trastuzumab-Coated Liposomes for Combating Head and Neck Squamous Cancer Cells</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7 </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Head and neck squamous cell carcinoma (HNSCC) with a high mortality rate is among the ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Head and neck squamous cell carcinoma (HNSCC) with a high mortality rate is among the most common types of cancer in the world. Human epidermal growth factor receptor 2 (HER2) is expressed higher than normal level in the most HNSCC tumors, making them resistant to chemotherapy and radiotherapy. Therefore, HER2 has been introduced as a suitable target for anticancer drugs. The aim of this study is to examine the efficacy of a treatment protocol involving targeted delivery of idarubicin encapsulated in trastuzumab-decorated liposomes to HNSCC cells. Materials and Methods: In the current experimental study, efficacies of idarubicin, prepared liposomal idarubicin, and constructed immunoliposomal idarubicin (trastuzumab-decorated) were investigated in killing HN5 cells, a HER2overexpressing HNSCC-originating cell line. Liposomal content of idarubicin and trastuzumab were qualified by UV-Visible spectroscopy and preparations were characterized for shape and size by atomic force microscopy (AFM) and dynamic light scattering (DLS). To clarify role of the missing parts of the available crystal structure (PDB ID: 1n8z) within trastuzumab-HER2 interactions, we used a 40 ns molecular dynamic simulation approach. Results: Based on the obtained results, liposomal idarubicin showed higher toxicity of the encapsulated drug on HN5 cells compared to the traditional free drug formulations. The immunoliposomal form of idarubicin was more effective than the liposomal formulation, in killing of HN5 cells. In addition, simulation of interactions between trastuzumab and HER2 revealed that the missing parts (in the crystal structure) of HER2 have critical interaction with trastuzumab, through salt-bridges and hydrogen bonds. Conclusion: It seems that the prepared immunoliposomes could attach more efficiently to HER2 overexpressing cells, which consequently leads to increasing cellular uptake of idarubicin through a receptor-mediated endocytosis mechanism. Moreover, simulation of the interaction between HER2 and trastuzumab suggested considerable possibilities for increasing trastuzumab affinity to HER2.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d4d6693bca59a9a91bcda91e44221f90" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201626,"asset_id":125097338,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201626/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097338"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097338"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097338; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097338]").text(description); $(".js-view-count[data-work-id=125097338]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097338; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097338']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d4d6693bca59a9a91bcda91e44221f90" } } $('.js-work-strip[data-work-id=125097338]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097338,"title":"Potential Advantages of Idarubicin-Loaded Trastuzumab-Coated Liposomes for Combating Head and Neck Squamous Cancer Cells","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2019704.1480","abstract":"Objective: Head and neck squamous cell carcinoma (HNSCC) with a high mortality rate is among the most common types of cancer in the world. Human epidermal growth factor receptor 2 (HER2) is expressed higher than normal level in the most HNSCC tumors, making them resistant to chemotherapy and radiotherapy. Therefore, HER2 has been introduced as a suitable target for anticancer drugs. The aim of this study is to examine the efficacy of a treatment protocol involving targeted delivery of idarubicin encapsulated in trastuzumab-decorated liposomes to HNSCC cells. Materials and Methods: In the current experimental study, efficacies of idarubicin, prepared liposomal idarubicin, and constructed immunoliposomal idarubicin (trastuzumab-decorated) were investigated in killing HN5 cells, a HER2overexpressing HNSCC-originating cell line. Liposomal content of idarubicin and trastuzumab were qualified by UV-Visible spectroscopy and preparations were characterized for shape and size by atomic force microscopy (AFM) and dynamic light scattering (DLS). To clarify role of the missing parts of the available crystal structure (PDB ID: 1n8z) within trastuzumab-HER2 interactions, we used a 40 ns molecular dynamic simulation approach. Results: Based on the obtained results, liposomal idarubicin showed higher toxicity of the encapsulated drug on HN5 cells compared to the traditional free drug formulations. The immunoliposomal form of idarubicin was more effective than the liposomal formulation, in killing of HN5 cells. In addition, simulation of interactions between trastuzumab and HER2 revealed that the missing parts (in the crystal structure) of HER2 have critical interaction with trastuzumab, through salt-bridges and hydrogen bonds. Conclusion: It seems that the prepared immunoliposomes could attach more efficiently to HER2 overexpressing cells, which consequently leads to increasing cellular uptake of idarubicin through a receptor-mediated endocytosis mechanism. Moreover, simulation of the interaction between HER2 and trastuzumab suggested considerable possibilities for increasing trastuzumab affinity to HER2.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7 "},"translated_abstract":"Objective: Head and neck squamous cell carcinoma (HNSCC) with a high mortality rate is among the most common types of cancer in the world. Human epidermal growth factor receptor 2 (HER2) is expressed higher than normal level in the most HNSCC tumors, making them resistant to chemotherapy and radiotherapy. Therefore, HER2 has been introduced as a suitable target for anticancer drugs. The aim of this study is to examine the efficacy of a treatment protocol involving targeted delivery of idarubicin encapsulated in trastuzumab-decorated liposomes to HNSCC cells. Materials and Methods: In the current experimental study, efficacies of idarubicin, prepared liposomal idarubicin, and constructed immunoliposomal idarubicin (trastuzumab-decorated) were investigated in killing HN5 cells, a HER2overexpressing HNSCC-originating cell line. Liposomal content of idarubicin and trastuzumab were qualified by UV-Visible spectroscopy and preparations were characterized for shape and size by atomic force microscopy (AFM) and dynamic light scattering (DLS). To clarify role of the missing parts of the available crystal structure (PDB ID: 1n8z) within trastuzumab-HER2 interactions, we used a 40 ns molecular dynamic simulation approach. Results: Based on the obtained results, liposomal idarubicin showed higher toxicity of the encapsulated drug on HN5 cells compared to the traditional free drug formulations. The immunoliposomal form of idarubicin was more effective than the liposomal formulation, in killing of HN5 cells. In addition, simulation of interactions between trastuzumab and HER2 revealed that the missing parts (in the crystal structure) of HER2 have critical interaction with trastuzumab, through salt-bridges and hydrogen bonds. Conclusion: It seems that the prepared immunoliposomes could attach more efficiently to HER2 overexpressing cells, which consequently leads to increasing cellular uptake of idarubicin through a receptor-mediated endocytosis mechanism. Moreover, simulation of the interaction between HER2 and trastuzumab suggested considerable possibilities for increasing trastuzumab affinity to HER2.","internal_url":"https://www.academia.edu/125097338/Potential_Advantages_of_Idarubicin_Loaded_Trastuzumab_Coated_Liposomes_for_Combating_Head_and_Neck_Squamous_Cancer_Cells","translated_internal_url":"","created_at":"2024-10-28T00:16:20.158-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615394,"work_id":125097338,"tagging_user_id":126987384,"tagged_user_id":58629343,"co_author_invite_id":null,"email":"m***m@gmail.com","display_order":1,"name":"mostafa jamalan","title":"Potential Advantages of Idarubicin-Loaded Trastuzumab-Coated Liposomes for Combating Head and Neck Squamous Cancer Cells"}],"downloadable_attachments":[{"id":119201626,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201626/thumbnails/1.jpg","file_name":"Cell_J_26_436.pdf","download_url":"https://www.academia.edu/attachments/119201626/download_file","bulk_download_file_name":"Potential_Advantages_of_Idarubicin_Loade.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201626/Cell_J_26_436-libre.pdf?1730101791=\u0026response-content-disposition=attachment%3B+filename%3DPotential_Advantages_of_Idarubicin_Loade.pdf\u0026Expires=1738763676\u0026Signature=LT9-P2~OPy4INV9xPuhTLBvMtUWG0L4hMQ5PX4kSNeazWXibOsymrCZxYV1ufQ5-rK~JltWke~P1y8nIFLawdAqDYuItT4uwp5dANBeP1T8fYxa5gsVYmqeT-hhIh7onzp~2S17nejqK8BvvJD9vzpaM1hQ-Q-3kMg5zDJTNfDpxiZxoravEycyt8MhxwzEdO6DNND7KRKayMP6fDFZ-8PWrg1F1lB2RZQrIgH56g7UDZhkbgI3R5NmreV6s4P7J853vg92hrALe9czk9ZmWL16SwwnPuL93eNruDUWeueGlpdg~td66VMsek3TAZPrEEtj0mlR-N3vC1s1etA8a9A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Potential_Advantages_of_Idarubicin_Loaded_Trastuzumab_Coated_Liposomes_for_Combating_Head_and_Neck_Squamous_Cancer_Cells","translated_slug":"","page_count":10,"language":"en","content_type":"Work","summary":"Objective: Head and neck squamous cell carcinoma (HNSCC) with a high mortality rate is among the most common types of cancer in the world. Human epidermal growth factor receptor 2 (HER2) is expressed higher than normal level in the most HNSCC tumors, making them resistant to chemotherapy and radiotherapy. Therefore, HER2 has been introduced as a suitable target for anticancer drugs. The aim of this study is to examine the efficacy of a treatment protocol involving targeted delivery of idarubicin encapsulated in trastuzumab-decorated liposomes to HNSCC cells. Materials and Methods: In the current experimental study, efficacies of idarubicin, prepared liposomal idarubicin, and constructed immunoliposomal idarubicin (trastuzumab-decorated) were investigated in killing HN5 cells, a HER2overexpressing HNSCC-originating cell line. Liposomal content of idarubicin and trastuzumab were qualified by UV-Visible spectroscopy and preparations were characterized for shape and size by atomic force microscopy (AFM) and dynamic light scattering (DLS). To clarify role of the missing parts of the available crystal structure (PDB ID: 1n8z) within trastuzumab-HER2 interactions, we used a 40 ns molecular dynamic simulation approach. Results: Based on the obtained results, liposomal idarubicin showed higher toxicity of the encapsulated drug on HN5 cells compared to the traditional free drug formulations. The immunoliposomal form of idarubicin was more effective than the liposomal formulation, in killing of HN5 cells. In addition, simulation of interactions between trastuzumab and HER2 revealed that the missing parts (in the crystal structure) of HER2 have critical interaction with trastuzumab, through salt-bridges and hydrogen bonds. Conclusion: It seems that the prepared immunoliposomes could attach more efficiently to HER2 overexpressing cells, which consequently leads to increasing cellular uptake of idarubicin through a receptor-mediated endocytosis mechanism. Moreover, simulation of the interaction between HER2 and trastuzumab suggested considerable possibilities for increasing trastuzumab affinity to HER2.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201626,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201626/thumbnails/1.jpg","file_name":"Cell_J_26_436.pdf","download_url":"https://www.academia.edu/attachments/119201626/download_file","bulk_download_file_name":"Potential_Advantages_of_Idarubicin_Loade.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201626/Cell_J_26_436-libre.pdf?1730101791=\u0026response-content-disposition=attachment%3B+filename%3DPotential_Advantages_of_Idarubicin_Loade.pdf\u0026Expires=1738763676\u0026Signature=LT9-P2~OPy4INV9xPuhTLBvMtUWG0L4hMQ5PX4kSNeazWXibOsymrCZxYV1ufQ5-rK~JltWke~P1y8nIFLawdAqDYuItT4uwp5dANBeP1T8fYxa5gsVYmqeT-hhIh7onzp~2S17nejqK8BvvJD9vzpaM1hQ-Q-3kMg5zDJTNfDpxiZxoravEycyt8MhxwzEdO6DNND7KRKayMP6fDFZ-8PWrg1F1lB2RZQrIgH56g7UDZhkbgI3R5NmreV6s4P7J853vg92hrALe9czk9ZmWL16SwwnPuL93eNruDUWeueGlpdg~td66VMsek3TAZPrEEtj0mlR-N3vC1s1etA8a9A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":6021,"name":"Cancer","url":"https://www.academia.edu/Documents/in/Cancer"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097144"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097144/Production_of_Cartilaginous_Organoids_Potential_Opportunities_and_Challenges_A_Review_Article"><img alt="Research paper thumbnail of Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article" class="work-thumbnail" src="https://attachments.academia-assets.com/119201498/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097144/Production_of_Cartilaginous_Organoids_Potential_Opportunities_and_Challenges_A_Review_Article">Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7 </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, and dysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment. Two dimensional (2D) and three-dimensional (3D) cell cultures can help to study growth modeling and physiological characteristics of the human body. Among the problems that 2D and single-layer cultures have the lack of proper and accurate tissue modeling and the lack of tissue complications similar is to the original tissue. With organoid models, cellular and tissue structural studies and functional and physiological studies of tissues have been revolutionized and more accurate. Organoids are useful for studying repair and drug efficacy. Physiological and pathological investigations by combining in vitro and in vivo methods have become more effective today. The purpose of this study is to investigate the factors involved in the formation of cartilage organoids so that we can introduce the best method of organoid production for the healing of cartilage damage by using cell types and organoid model.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bf7cac054c7a1dbb0ae19d16a2213693" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201498,"asset_id":125097144,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201498/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097144"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097144"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097144; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097144]").text(description); $(".js-view-count[data-work-id=125097144]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097144; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097144']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bf7cac054c7a1dbb0ae19d16a2213693" } } $('.js-work-strip[data-work-id=125097144]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097144,"title":"Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2023118.1510","abstract":"Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, and dysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment. Two dimensional (2D) and three-dimensional (3D) cell cultures can help to study growth modeling and physiological characteristics of the human body. Among the problems that 2D and single-layer cultures have the lack of proper and accurate tissue modeling and the lack of tissue complications similar is to the original tissue. With organoid models, cellular and tissue structural studies and functional and physiological studies of tissues have been revolutionized and more accurate. Organoids are useful for studying repair and drug efficacy. Physiological and pathological investigations by combining in vitro and in vivo methods have become more effective today. The purpose of this study is to investigate the factors involved in the formation of cartilage organoids so that we can introduce the best method of organoid production for the healing of cartilage damage by using cell types and organoid model.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7 "},"translated_abstract":"Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, and dysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment. Two dimensional (2D) and three-dimensional (3D) cell cultures can help to study growth modeling and physiological characteristics of the human body. Among the problems that 2D and single-layer cultures have the lack of proper and accurate tissue modeling and the lack of tissue complications similar is to the original tissue. With organoid models, cellular and tissue structural studies and functional and physiological studies of tissues have been revolutionized and more accurate. Organoids are useful for studying repair and drug efficacy. Physiological and pathological investigations by combining in vitro and in vivo methods have become more effective today. The purpose of this study is to investigate the factors involved in the formation of cartilage organoids so that we can introduce the best method of organoid production for the healing of cartilage damage by using cell types and organoid model.","internal_url":"https://www.academia.edu/125097144/Production_of_Cartilaginous_Organoids_Potential_Opportunities_and_Challenges_A_Review_Article","translated_internal_url":"","created_at":"2024-10-28T00:09:51.349-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615386,"work_id":125097144,"tagging_user_id":126987384,"tagged_user_id":null,"co_author_invite_id":8272068,"email":"a***i@sums.ac.ir","display_order":1,"name":"Ali Rajabi","title":"Production of Cartilaginous Organoids: Potential Opportunities and Challenges, A Review Article"}],"downloadable_attachments":[{"id":119201498,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201498/thumbnails/1.jpg","file_name":"Cell_J_26_427.pdf","download_url":"https://www.academia.edu/attachments/119201498/download_file","bulk_download_file_name":"Production_of_Cartilaginous_Organoids_Po.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201498/Cell_J_26_427-libre.pdf?1730101804=\u0026response-content-disposition=attachment%3B+filename%3DProduction_of_Cartilaginous_Organoids_Po.pdf\u0026Expires=1738763676\u0026Signature=LYDq9hx1I6qT~T2Hp-ysmq6kko7LaGw8cpDnZ6oAEt-iLnUvz9jDvZjXY87T21HSyIk-EG4OdUJtDv80CiaNOni0mvGNz2e0Gk6NthVMWyePAw1q9~1SEuPTo1URNzboV~E61dQSwOD~Ew7Fi9M-WGG5-m5-3fvZmSAbg51t6GvYtujRKAy2Run-w0skH057NLOeeNOaHEzXotqwYYz8Ys2Cw4MbvIqS6pV3Mv4pZqBSa60xyIdfUszMO95v9qkdf2avUlUhlinQ-9F9mXyzhx8YzWtGxC6qn8aUDKIq4yeuSowrX6qoe5Ugwct~M4Z9SgEM2X3yWgr0K0GV2pE2LA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Production_of_Cartilaginous_Organoids_Potential_Opportunities_and_Challenges_A_Review_Article","translated_slug":"","page_count":9,"language":"en","content_type":"Work","summary":"Damaged articular cartilage has limited self-healing potential and often leads to osteoarthritis (OA), pain, and dysfunction of the affected joint. Autologous and allogenic transplants cannot fully meet the needs of clinical treatment. Two dimensional (2D) and three-dimensional (3D) cell cultures can help to study growth modeling and physiological characteristics of the human body. Among the problems that 2D and single-layer cultures have the lack of proper and accurate tissue modeling and the lack of tissue complications similar is to the original tissue. With organoid models, cellular and tissue structural studies and functional and physiological studies of tissues have been revolutionized and more accurate. Organoids are useful for studying repair and drug efficacy. Physiological and pathological investigations by combining in vitro and in vivo methods have become more effective today. The purpose of this study is to investigate the factors involved in the formation of cartilage organoids so that we can introduce the best method of organoid production for the healing of cartilage damage by using cell types and organoid model.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201498,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201498/thumbnails/1.jpg","file_name":"Cell_J_26_427.pdf","download_url":"https://www.academia.edu/attachments/119201498/download_file","bulk_download_file_name":"Production_of_Cartilaginous_Organoids_Po.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201498/Cell_J_26_427-libre.pdf?1730101804=\u0026response-content-disposition=attachment%3B+filename%3DProduction_of_Cartilaginous_Organoids_Po.pdf\u0026Expires=1738763676\u0026Signature=LYDq9hx1I6qT~T2Hp-ysmq6kko7LaGw8cpDnZ6oAEt-iLnUvz9jDvZjXY87T21HSyIk-EG4OdUJtDv80CiaNOni0mvGNz2e0Gk6NthVMWyePAw1q9~1SEuPTo1URNzboV~E61dQSwOD~Ew7Fi9M-WGG5-m5-3fvZmSAbg51t6GvYtujRKAy2Run-w0skH057NLOeeNOaHEzXotqwYYz8Ys2Cw4MbvIqS6pV3Mv4pZqBSa60xyIdfUszMO95v9qkdf2avUlUhlinQ-9F9mXyzhx8YzWtGxC6qn8aUDKIq4yeuSowrX6qoe5Ugwct~M4Z9SgEM2X3yWgr0K0GV2pE2LA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":13827,"name":"Cell Biology","url":"https://www.academia.edu/Documents/in/Cell_Biology"},{"id":78188,"name":"Cartilage","url":"https://www.academia.edu/Documents/in/Cartilage"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="125097065"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125097065/A_Comparative_Analysis_of_Computational_Strategies_in_Multi_Epitope_Vaccine_Design_Against_Human_Papillomavirus_and_Cervical_Cancer"><img alt="Research paper thumbnail of A Comparative Analysis of Computational Strategies in Multi-Epitope Vaccine Design Against Human Papillomavirus and Cervical Cancer" class="work-thumbnail" src="https://attachments.academia-assets.com/119201419/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/125097065/A_Comparative_Analysis_of_Computational_Strategies_in_Multi_Epitope_Vaccine_Design_Against_Human_Papillomavirus_and_Cervical_Cancer">A Comparative Analysis of Computational Strategies in Multi-Epitope Vaccine Design Against Human Papillomavirus and Cervical Cancer</a></div><div class="wp-workCard_item"><span>Volume 26, Issue 7</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other m...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other malignancies, there is a need for innovative approaches to preventing this infection. It has been shown that immunoinformatics is an important strategy in computational vaccinology. It is used to design new multi-epitope vaccines against different types of HPV and subsequent cervical cancer. This paper reviews the scope of the entire computational pipeline of HPV vaccine design, starting from data analysis at the genomic and proteomic levels and continuing to epitope predictions of the innate and adaptive immune systems. The search strategy was based on investigating original articles published in "Google Scholar" and "PubMed" from 2015 to 2023-2024. The terms "Immunoinformatics", "Bioinformatics", "Human papillomavirus (HPV)", "Vaccine design", "In silico vaccine design", "Multi-epitope vaccine design", "Vaccinology" and "HPV vaccine" were used to for this purpose. We discussed various essential tools involved in the computational design of the vaccine process, e.g., sequence analysis, epitope prediction, conservancy analysis, tertiary structure modeling, refinement, molecular docking, molecular dynamics (MD) simulation, and in silico cloning. This review article describes immunoinformatics methods that facilitate the design of a multi-epitope vaccine against HPV. However, this pipeline can also be used to design novel chimeric vaccines for other pathogens.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1a9fae34cccf3bd3471946fcd911c114" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":119201419,"asset_id":125097065,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/119201419/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="125097065"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="125097065"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 125097065; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=125097065]").text(description); $(".js-view-count[data-work-id=125097065]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 125097065; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='125097065']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1a9fae34cccf3bd3471946fcd911c114" } } $('.js-work-strip[data-work-id=125097065]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":125097065,"title":"A Comparative Analysis of Computational Strategies in Multi-Epitope Vaccine Design Against Human Papillomavirus and Cervical Cancer","translated_title":"","metadata":{"doi":"10.22074/cellj.2024.2028622.1568","abstract":"Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other malignancies, there is a need for innovative approaches to preventing this infection. It has been shown that immunoinformatics is an important strategy in computational vaccinology. It is used to design new multi-epitope vaccines against different types of HPV and subsequent cervical cancer. This paper reviews the scope of the entire computational pipeline of HPV vaccine design, starting from data analysis at the genomic and proteomic levels and continuing to epitope predictions of the innate and adaptive immune systems. The search strategy was based on investigating original articles published in \"Google Scholar\" and \"PubMed\" from 2015 to 2023-2024. The terms \"Immunoinformatics\", \"Bioinformatics\", \"Human papillomavirus (HPV)\", \"Vaccine design\", \"In silico vaccine design\", \"Multi-epitope vaccine design\", \"Vaccinology\" and \"HPV vaccine\" were used to for this purpose. We discussed various essential tools involved in the computational design of the vaccine process, e.g., sequence analysis, epitope prediction, conservancy analysis, tertiary structure modeling, refinement, molecular docking, molecular dynamics (MD) simulation, and in silico cloning. This review article describes immunoinformatics methods that facilitate the design of a multi-epitope vaccine against HPV. However, this pipeline can also be used to design novel chimeric vaccines for other pathogens.","ai_title_tag":"Immunoinformatics for HPV Multi-Epitope Vaccines","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Volume 26, Issue 7"},"translated_abstract":"Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other malignancies, there is a need for innovative approaches to preventing this infection. It has been shown that immunoinformatics is an important strategy in computational vaccinology. It is used to design new multi-epitope vaccines against different types of HPV and subsequent cervical cancer. This paper reviews the scope of the entire computational pipeline of HPV vaccine design, starting from data analysis at the genomic and proteomic levels and continuing to epitope predictions of the innate and adaptive immune systems. The search strategy was based on investigating original articles published in \"Google Scholar\" and \"PubMed\" from 2015 to 2023-2024. The terms \"Immunoinformatics\", \"Bioinformatics\", \"Human papillomavirus (HPV)\", \"Vaccine design\", \"In silico vaccine design\", \"Multi-epitope vaccine design\", \"Vaccinology\" and \"HPV vaccine\" were used to for this purpose. We discussed various essential tools involved in the computational design of the vaccine process, e.g., sequence analysis, epitope prediction, conservancy analysis, tertiary structure modeling, refinement, molecular docking, molecular dynamics (MD) simulation, and in silico cloning. This review article describes immunoinformatics methods that facilitate the design of a multi-epitope vaccine against HPV. However, this pipeline can also be used to design novel chimeric vaccines for other pathogens.","internal_url":"https://www.academia.edu/125097065/A_Comparative_Analysis_of_Computational_Strategies_in_Multi_Epitope_Vaccine_Design_Against_Human_Papillomavirus_and_Cervical_Cancer","translated_internal_url":"","created_at":"2024-10-28T00:06:16.236-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42615377,"work_id":125097065,"tagging_user_id":126987384,"tagged_user_id":null,"co_author_invite_id":8272066,"email":"e***h@bmsu.ac.ir","display_order":1,"name":"Hadi Esmaeili","title":"A Comparative Analysis of Computational Strategies in Multi-Epitope Vaccine Design Against Human Papillomavirus and Cervical Cancer"}],"downloadable_attachments":[{"id":119201419,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201419/thumbnails/1.jpg","file_name":"Cell_J_26_403.pdf","download_url":"https://www.academia.edu/attachments/119201419/download_file","bulk_download_file_name":"A_Comparative_Analysis_of_Computational.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201419/Cell_J_26_403-libre.pdf?1730104274=\u0026response-content-disposition=attachment%3B+filename%3DA_Comparative_Analysis_of_Computational.pdf\u0026Expires=1738725410\u0026Signature=cD2YafcVymRm3pDKncg2qtsEEDDuDMZUZZ1bnrjL-DtzfOhRdeF-rg-9dZqKYYvlyfgkGEus2CeDpOUxjE4~3VbVWKxivEqfbtH1A7UxvOVcGif5YZLO1GfRHcGhBgPL6jiYUjohzLIWhDOsRlqdh59FVlyO66XbJ8S2EipWTz0vQsEWOJ3lJsAOkdO4nvL0~T7f6MDSsPMlErP~XWhGLoS~kRbpoXNAaN2vnt4-Nzq057~vriG0zRRIuyzKL7Re81~w0abCtpIDVfLN3Mm7bGhTd1tt3cx8O8m1PifUJjphxI4X1E5lnShav0zUEpVniW0yI7pq1j~2xVm9dvF5yg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_Comparative_Analysis_of_Computational_Strategies_in_Multi_Epitope_Vaccine_Design_Against_Human_Papillomavirus_and_Cervical_Cancer","translated_slug":"","page_count":24,"language":"en","content_type":"Work","summary":"Given the critical role of human papillomavirus (HPV) in the cause of cervical cancer and other malignancies, there is a need for innovative approaches to preventing this infection. It has been shown that immunoinformatics is an important strategy in computational vaccinology. It is used to design new multi-epitope vaccines against different types of HPV and subsequent cervical cancer. This paper reviews the scope of the entire computational pipeline of HPV vaccine design, starting from data analysis at the genomic and proteomic levels and continuing to epitope predictions of the innate and adaptive immune systems. The search strategy was based on investigating original articles published in \"Google Scholar\" and \"PubMed\" from 2015 to 2023-2024. The terms \"Immunoinformatics\", \"Bioinformatics\", \"Human papillomavirus (HPV)\", \"Vaccine design\", \"In silico vaccine design\", \"Multi-epitope vaccine design\", \"Vaccinology\" and \"HPV vaccine\" were used to for this purpose. We discussed various essential tools involved in the computational design of the vaccine process, e.g., sequence analysis, epitope prediction, conservancy analysis, tertiary structure modeling, refinement, molecular docking, molecular dynamics (MD) simulation, and in silico cloning. This review article describes immunoinformatics methods that facilitate the design of a multi-epitope vaccine against HPV. However, this pipeline can also be used to design novel chimeric vaccines for other pathogens.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":119201419,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/119201419/thumbnails/1.jpg","file_name":"Cell_J_26_403.pdf","download_url":"https://www.academia.edu/attachments/119201419/download_file","bulk_download_file_name":"A_Comparative_Analysis_of_Computational.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/119201419/Cell_J_26_403-libre.pdf?1730104274=\u0026response-content-disposition=attachment%3B+filename%3DA_Comparative_Analysis_of_Computational.pdf\u0026Expires=1738725410\u0026Signature=cD2YafcVymRm3pDKncg2qtsEEDDuDMZUZZ1bnrjL-DtzfOhRdeF-rg-9dZqKYYvlyfgkGEus2CeDpOUxjE4~3VbVWKxivEqfbtH1A7UxvOVcGif5YZLO1GfRHcGhBgPL6jiYUjohzLIWhDOsRlqdh59FVlyO66XbJ8S2EipWTz0vQsEWOJ3lJsAOkdO4nvL0~T7f6MDSsPMlErP~XWhGLoS~kRbpoXNAaN2vnt4-Nzq057~vriG0zRRIuyzKL7Re81~w0abCtpIDVfLN3Mm7bGhTd1tt3cx8O8m1PifUJjphxI4X1E5lnShav0zUEpVniW0yI7pq1j~2xVm9dvF5yg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":50351,"name":"HUMAN PAPILLOMAVIRUS","url":"https://www.academia.edu/Documents/in/HUMAN_PAPILLOMAVIRUS"},{"id":173523,"name":"HPV","url":"https://www.academia.edu/Documents/in/HPV"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123077776"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123077776/Conditioned_Medium_Protects_Dopaminergic_Neurons_in_Parkinsonian_Rats"><img alt="Research paper thumbnail of Conditioned Medium Protects Dopaminergic Neurons in Parkinsonian Rats" class="work-thumbnail" src="https://attachments.academia-assets.com/117598261/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123077776/Conditioned_Medium_Protects_Dopaminergic_Neurons_in_Parkinsonian_Rats">Conditioned Medium Protects Dopaminergic Neurons in Parkinsonian Rats</a></div><div class="wp-workCard_item"><span>Cell journal</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditio...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditioned medium (CM), which protect neurons by its antioxidative and trophic effects. This research assesses the neuroprotective effect of ASCCM on neurotrophins genes expressions and tyrosine hydroxylase positive (TH) cell density in male Wistar rats lesioned by 6-hydroxydopamine (6-OHDA). In this experimental study, the groups consisted of lesioned and sham rats with unilateral injections of 20 μg of 6-OHDA neurotoxin and phosphate buffered saline (PBS) into the striatum, respectively. Another groups received intravenous injections of 3×106 cells (ASCs group), 500 μl of CM (ASC-CM group) or medium [α-minimal essential medium (α-MEM) group)]. All rats underwent evaluations with the rotarod and apomorphine-induced rotation tests at 2, 4, 6, and 8 weeks post-injection. At 8 weeks we sacrificed some of the animals for real-time polymerase chain reaction (PCR) analysis, and evaluation of TH cell...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b267615cd882d29a773989703c5674d2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117598261,"asset_id":123077776,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117598261/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123077776"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123077776"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123077776; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123077776]").text(description); $(".js-view-count[data-work-id=123077776]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123077776; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123077776']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b267615cd882d29a773989703c5674d2" } } $('.js-work-strip[data-work-id=123077776]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123077776,"title":"Conditioned Medium Protects Dopaminergic Neurons in Parkinsonian Rats","translated_title":"","metadata":{"abstract":"Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditioned medium (CM), which protect neurons by its antioxidative and trophic effects. This research assesses the neuroprotective effect of ASCCM on neurotrophins genes expressions and tyrosine hydroxylase positive (TH) cell density in male Wistar rats lesioned by 6-hydroxydopamine (6-OHDA). In this experimental study, the groups consisted of lesioned and sham rats with unilateral injections of 20 μg of 6-OHDA neurotoxin and phosphate buffered saline (PBS) into the striatum, respectively. Another groups received intravenous injections of 3×106 cells (ASCs group), 500 μl of CM (ASC-CM group) or medium [α-minimal essential medium (α-MEM) group)]. All rats underwent evaluations with the rotarod and apomorphine-induced rotation tests at 2, 4, 6, and 8 weeks post-injection. At 8 weeks we sacrificed some of the animals for real-time polymerase chain reaction (PCR) analysis, and evaluation of TH cell...","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"Cell journal"},"translated_abstract":"Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditioned medium (CM), which protect neurons by its antioxidative and trophic effects. This research assesses the neuroprotective effect of ASCCM on neurotrophins genes expressions and tyrosine hydroxylase positive (TH) cell density in male Wistar rats lesioned by 6-hydroxydopamine (6-OHDA). In this experimental study, the groups consisted of lesioned and sham rats with unilateral injections of 20 μg of 6-OHDA neurotoxin and phosphate buffered saline (PBS) into the striatum, respectively. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123077647"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123077647/Exploring_Advances_in_Reproduction_and_Stem_Cell_Biology_Highlights_from_The_24th_and_19th_International_Congresses_in_Iran"><img alt="Research paper thumbnail of Exploring Advances in Reproduction and Stem Cell Biology: Highlights from The 24th and 19th International Congresses in Iran" class="work-thumbnail" src="https://attachments.academia-assets.com/117598128/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123077647/Exploring_Advances_in_Reproduction_and_Stem_Cell_Biology_Highlights_from_The_24th_and_19th_International_Congresses_in_Iran">Exploring Advances in Reproduction and Stem Cell Biology: Highlights from The 24th and 19th International Congresses in Iran</a></div><div class="wp-workCard_item"><span>Vol 26, No 6, June </span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The 24th and 19th International Congresses on Reproduction and Stem Cell Biology in the Islamic R...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The 24th and 19th International Congresses on Reproduction and Stem Cell Biology in the Islamic Republic of Iran brought<br />together experts and researchers worldwide to explore the latest advancements in these fields. Different topics were<br />discussed, including such as reproductive health, infertility treatments, stem cell research, and regenerative medicine.<br />This report provides a summary of the congress’s key findings by emphasizing pioneer research and technologies that<br />can influence the future of reproduction and stem cell biology programs. The presence of keynote speakers such as<br />Professor Nicolas Rivron, Mohammad Ebrahim Parsanezhad, Ashraf Moini, Abbas Aflatoonian, Hadi Shafiee, Anna<br />Brini, Omid Camron Farokhzad, and Jeffrey Schweitzer added value to the event, which had over 1100 participants<br />from around the world. While foreign speakers were from various countries Iranian speakers mainly came from Tabriz,<br />Isfahan, Shiraz, Babol, and Tehran that all discussed cutting-edge science and successful disease treatments. To<br />ensure a more comprehensive representation, it is suggested that a wider geographic distribution of national and<br />foreign speakers should be considered in future plan.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="982e64b51828f4e09269c97d6b94432a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117598128,"asset_id":123077647,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117598128/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123077647"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123077647"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123077647; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123077647]").text(description); $(".js-view-count[data-work-id=123077647]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123077647; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123077647']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "982e64b51828f4e09269c97d6b94432a" } } $('.js-work-strip[data-work-id=123077647]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123077647,"title":"Exploring Advances in Reproduction and Stem Cell Biology: Highlights from The 24th and 19th International Congresses in Iran","translated_title":"","metadata":{"doi":"10.22074/CELLJ.2024.2027233.1559","abstract":"The 24th and 19th International Congresses on Reproduction and Stem Cell Biology in the Islamic Republic of Iran brought\ntogether experts and researchers worldwide to explore the latest advancements in these fields. 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Focusing on an Iranian family exhibiting USH2 symptoms, exome-sequencing was employed for a comprehensive genome analysis in a 30-yearold patient. The investigation unveiled a novel variation (NM_206933.4: c.9389G>A; p.Trp3130*) within exon 48 of the USH2A gene, a previously unreported variant emphasizing the genetic diversity in USH2. Sanger sequencing was then utilized to assess variation segregation within the family, offering insights into the inheritance pattern. This discovery not only advances our understanding of the genetic basis of USH2 but also holds significant implications for genetic counseling, early management, and informed decision-making regarding prenatal options. By adopting an integrated approach, this study aims to empower affected families, facilitating a nuanced understanding of the disorder's complexities and ultimately improving patient outcomes and family well-being through informed decisionmaking and proactive management strategies.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7769c1248c0c11c3b6092c55ce6cadd8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117598103,"asset_id":123077610,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117598103/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123077610"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123077610"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123077610; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123077610]").text(description); $(".js-view-count[data-work-id=123077610]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123077610; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123077610']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7769c1248c0c11c3b6092c55ce6cadd8" } } $('.js-work-strip[data-work-id=123077610]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123077610,"title":"Usher Syndrome Type 2 in An Iranian Family: A Novel Founder Variation in The USH2A Gene","translated_title":"","metadata":{"doi":"10.22074/CELLJ.2024.2024223.1521","abstract":"This study delves into Usher syndrome type 2 (USH2), an uncommon genetic disorder characterized by sensorineural hearing loss (HL) and retinitis pigmentosa (RP), often associated with the USH2A gene. Focusing on an Iranian family exhibiting USH2 symptoms, exome-sequencing was employed for a comprehensive genome analysis in a 30-yearold patient. The investigation unveiled a novel variation (NM_206933.4: c.9389G\u003eA; p.Trp3130*) within exon 48 of the USH2A gene, a previously unreported variant emphasizing the genetic diversity in USH2. Sanger sequencing was then utilized to assess variation segregation within the family, offering insights into the inheritance pattern. This discovery not only advances our understanding of the genetic basis of USH2 but also holds significant implications for genetic counseling, early management, and informed decision-making regarding prenatal options. By adopting an integrated approach, this study aims to empower affected families, facilitating a nuanced understanding of the disorder's complexities and ultimately improving patient outcomes and family well-being through informed decisionmaking and proactive management strategies.","publication_date":{"day":null,"month":null,"year":2024,"errors":{}},"publication_name":"Vol 26, No 6, June "},"translated_abstract":"This study delves into Usher syndrome type 2 (USH2), an uncommon genetic disorder characterized by sensorineural hearing loss (HL) and retinitis pigmentosa (RP), often associated with the USH2A gene. Focusing on an Iranian family exhibiting USH2 symptoms, exome-sequencing was employed for a comprehensive genome analysis in a 30-yearold patient. The investigation unveiled a novel variation (NM_206933.4: c.9389G\u003eA; p.Trp3130*) within exon 48 of the USH2A gene, a previously unreported variant emphasizing the genetic diversity in USH2. 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By adopting an integrated approach, this study aims to empower affected families, facilitating a nuanced understanding of the disorder's complexities and ultimately improving patient outcomes and family well-being through informed decisionmaking and proactive management strategies.","internal_url":"https://www.academia.edu/123077610/Usher_Syndrome_Type_2_in_An_Iranian_Family_A_Novel_Founder_Variation_in_The_USH2A_Gene","translated_internal_url":"","created_at":"2024-08-20T23:40:40.107-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":126987384,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":42255297,"work_id":123077610,"tagging_user_id":126987384,"tagged_user_id":112722747,"co_author_invite_id":null,"email":"i***i@gmail.com","display_order":1,"name":"Mostafa Neissi","title":"Usher Syndrome Type 2 in An Iranian Family: A Novel Founder Variation in The USH2A Gene"}],"downloadable_attachments":[{"id":117598103,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117598103/thumbnails/1.jpg","file_name":"Cell_J_26_392.pdf","download_url":"https://www.academia.edu/attachments/117598103/download_file","bulk_download_file_name":"Usher_Syndrome_Type_2_in_An_Iranian_Fami.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117598103/Cell_J_26_392-libre.pdf?1724224288=\u0026response-content-disposition=attachment%3B+filename%3DUsher_Syndrome_Type_2_in_An_Iranian_Fami.pdf\u0026Expires=1738763676\u0026Signature=VDhvjunmWzwEZIx4pm9ifvr~cG8AgHptRl46Kp8U4-7~J3dv81Xs2wpFQXVawgMt9HH04faOwtJQ9ifjeLN~72-8KGKkgDAhuYpvH8T7IH1l6kZJrmWitaWzZNF-0hWTqb-8BlxOn0TZCIoYNzJ4eyPaNnrtWNmqURN6r5DzTlo5OitDqSqKYV99T~A18LD40-wsAywlQHXd1Z9~DSMjNJXy6tXJLv7AE2ckQgzF4Ssnqi0xoInUMr6-wbL8eEfWJikoFPuASx8TxiYRmo-YKrsPM3DjPLda2aRigeWQAITgeqjHn9MKSLM7dxrLfSDZETL70Gb0v~yNtNf~aXUlYg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Usher_Syndrome_Type_2_in_An_Iranian_Family_A_Novel_Founder_Variation_in_The_USH2A_Gene","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"This study delves into Usher syndrome type 2 (USH2), an uncommon genetic disorder characterized by sensorineural hearing loss (HL) and retinitis pigmentosa (RP), often associated with the USH2A gene. 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By adopting an integrated approach, this study aims to empower affected families, facilitating a nuanced understanding of the disorder's complexities and ultimately improving patient outcomes and family well-being through informed decisionmaking and proactive management strategies.","owner":{"id":126987384,"first_name":"Cell Journal","middle_initials":null,"last_name":"Yakhteh","page_name":"CellJournalYakhteh","domain_name":"royaninstitute","created_at":"2019-09-18T03:26:56.226-07:00","display_name":"Cell Journal Yakhteh","url":"https://royaninstitute.academia.edu/CellJournalYakhteh"},"attachments":[{"id":117598103,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117598103/thumbnails/1.jpg","file_name":"Cell_J_26_392.pdf","download_url":"https://www.academia.edu/attachments/117598103/download_file","bulk_download_file_name":"Usher_Syndrome_Type_2_in_An_Iranian_Fami.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117598103/Cell_J_26_392-libre.pdf?1724224288=\u0026response-content-disposition=attachment%3B+filename%3DUsher_Syndrome_Type_2_in_An_Iranian_Fami.pdf\u0026Expires=1738763676\u0026Signature=VDhvjunmWzwEZIx4pm9ifvr~cG8AgHptRl46Kp8U4-7~J3dv81Xs2wpFQXVawgMt9HH04faOwtJQ9ifjeLN~72-8KGKkgDAhuYpvH8T7IH1l6kZJrmWitaWzZNF-0hWTqb-8BlxOn0TZCIoYNzJ4eyPaNnrtWNmqURN6r5DzTlo5OitDqSqKYV99T~A18LD40-wsAywlQHXd1Z9~DSMjNJXy6tXJLv7AE2ckQgzF4Ssnqi0xoInUMr6-wbL8eEfWJikoFPuASx8TxiYRmo-YKrsPM3DjPLda2aRigeWQAITgeqjHn9MKSLM7dxrLfSDZETL70Gb0v~yNtNf~aXUlYg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1225171,"name":"Syndrome","url":"https://www.academia.edu/Documents/in/Syndrome"},{"id":1824390,"name":"Usher","url":"https://www.academia.edu/Documents/in/Usher"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123077579"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123077579/Tramadol_Influences_Cellular_Metabolism_and_Autophagy_in_Germ_and_Sertoli_Cells_Exercise_Training_as_A_Potential_Ameliorative_Strategy"><img alt="Research paper thumbnail of Tramadol Influences Cellular Metabolism and Autophagy in Germ and Sertoli Cells; Exercise Training as A Potential Ameliorative Strategy" class="work-thumbnail" src="https://attachments.academia-assets.com/117598082/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123077579/Tramadol_Influences_Cellular_Metabolism_and_Autophagy_in_Germ_and_Sertoli_Cells_Exercise_Training_as_A_Potential_Ameliorative_Strategy">Tramadol Influences Cellular Metabolism and Autophagy in Germ and Sertoli Cells; Exercise Training as A Potential Ameliorative Strategy</a></div><div class="wp-workCard_item"><span>Vol 26, No 6, June</span><span>, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective: Dysregulation of lipid and carbohydrate/fatty acid (FA) balance in Sertoli and germ ce...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective: Dysregulation of lipid and carbohydrate/fatty acid (FA) balance in Sertoli and germ cells alters the NADP + / NADPH ratio, resulting in metabolic autophagy in testicles. Tramadol (TRA) adversely affects spermatogenesis development, and it is not reversed within short periods of time after withdrawal. Therefore, the present study aimed to examine the boosting effect of different exercise training protocols (ETPs) on TRA-induced detrimental effects after withdrawal. Materials and Methods: In this experimental study, 36 mature Wistar rats were separated into control and TRAsole (administered 40 mg/kg of TRA and euthanized 60 days after TRA administration), Con-TRA (stopped TRA administration after 60 days, and continued for additional 60 days after withdrawal), TRA+low-intensity (TRA+LICT), TRA + moderate-intensity (TRA+MICT), and TRA+high-intensity continuous (TRA+HICT) ETPs-induced groups (n=6/ group, ETPs were initiated for 60 days after stopping TRA administration). Next, the intracytoplasmic carbohydrate and lipids/FAs content, testicular lactate and lactate dehydrogenase (LDH) levels, relative ratios of NADP + /NADPH, serum testosterone levels, and the Leydig cells steroidogenic activity, the mRNA levels of Beclin-1, p62, LC3-I, and Atg7 as well as the LC3-I/II + germ and somatic cells mean distributions were analyzed. Results: The LICT and MICT could ameliorate the TRA-induced carbohydrates/lipids, FAs imbalance, increase lactate, LDH and testosterone levels, re-balance the NADP + /NADPH ratio, and reregulate the autophagy and steroidogenic activities in the Leydig and Sertoli cells. Conclusion: Collectively, LICT and MICT can ameliorate the TRA-induced metabolic-oxidative autophagy by rebalancing energy survey in testicles and down-regulating autophagy reactions in Sertoli cells and rebalancing it in the Leydig cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="839c54782b2115fc9adbd494404b734d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117598082,"asset_id":123077579,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117598082/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123077579"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123077579"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123077579; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123077579]").text(description); $(".js-view-count[data-work-id=123077579]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123077579; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123077579']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "839c54782b2115fc9adbd494404b734d" } } $('.js-work-strip[data-work-id=123077579]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123077579,"title":"Tramadol Influences Cellular Metabolism and Autophagy in Germ and Sertoli Cells; Exercise Training as A Potential Ameliorative Strategy","translated_title":"","metadata":{"doi":"10.22074/CELLJ.2024.2024852.1532","abstract":"Objective: Dysregulation of lipid and carbohydrate/fatty acid (FA) balance in Sertoli and germ cells alters the NADP + / NADPH ratio, resulting in metabolic autophagy in testicles. Tramadol (TRA) adversely affects spermatogenesis development, and it is not reversed within short periods of time after withdrawal. Therefore, the present study aimed to examine the boosting effect of different exercise training protocols (ETPs) on TRA-induced detrimental effects after withdrawal. Materials and Methods: In this experimental study, 36 mature Wistar rats were separated into control and TRAsole (administered 40 mg/kg of TRA and euthanized 60 days after TRA administration), Con-TRA (stopped TRA administration after 60 days, and continued for additional 60 days after withdrawal), TRA+low-intensity (TRA+LICT), TRA + moderate-intensity (TRA+MICT), and TRA+high-intensity continuous (TRA+HICT) ETPs-induced groups (n=6/ group, ETPs were initiated for 60 days after stopping TRA administration). Next, the intracytoplasmic carbohydrate and lipids/FAs content, testicular lactate and lactate dehydrogenase (LDH) levels, relative ratios of NADP + /NADPH, serum testosterone levels, and the Leydig cells steroidogenic activity, the mRNA levels of Beclin-1, p62, LC3-I, and Atg7 as well as the LC3-I/II + germ and somatic cells mean distributions were analyzed. Results: The LICT and MICT could ameliorate the TRA-induced carbohydrates/lipids, FAs imbalance, increase lactate, LDH and testosterone levels, re-balance the NADP + /NADPH ratio, and reregulate the autophagy and steroidogenic activities in the Leydig and Sertoli cells. 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Materials and Methods: In this experimental study, 36 mature Wistar rats were separated into control and TRAsole (administered 40 mg/kg of TRA and euthanized 60 days after TRA administration), Con-TRA (stopped TRA administration after 60 days, and continued for additional 60 days after withdrawal), TRA+low-intensity (TRA+LICT), TRA + moderate-intensity (TRA+MICT), and TRA+high-intensity continuous (TRA+HICT) ETPs-induced groups (n=6/ group, ETPs were initiated for 60 days after stopping TRA administration). Next, the intracytoplasmic carbohydrate and lipids/FAs content, testicular lactate and lactate dehydrogenase (LDH) levels, relative ratios of NADP + /NADPH, serum testosterone levels, and the Leydig cells steroidogenic activity, the mRNA levels of Beclin-1, p62, LC3-I, and Atg7 as well as the LC3-I/II + germ and somatic cells mean distributions were analyzed. 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Next, the intracytoplasmic carbohydrate and lipids/FAs content, testicular lactate and lactate dehydrogenase (LDH) levels, relative ratios of NADP + /NADPH, serum testosterone levels, and the Leydig cells steroidogenic activity, the mRNA levels of Beclin-1, p62, LC3-I, and Atg7 as well as the LC3-I/II + germ and somatic cells mean distributions were analyzed. Results: The LICT and MICT could ameliorate the TRA-induced carbohydrates/lipids, FAs imbalance, increase lactate, LDH and testosterone levels, re-balance the NADP + /NADPH ratio, and reregulate the autophagy and steroidogenic activities in the Leydig and Sertoli cells. 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