Automated Western Blot Quantification with Simple Western

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<div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><ul> <li><strong>Are you looking for reliable absolute or relative quantitation of your target?</strong></li> <li><strong>Do you want to simultaneously detect high and low protein concentrations?</strong></li> </ul> <h2>Quantitative Results with Simple Western</h2> <p>Our <a href="/instruments/simple-western">Simple Western™</a> systems give you the detection and quantitation you need for reliable results. Reliably detect signal saturation and work within the linear dynamic range of your assay. With HDR detection profile and analysis Simple Western gets you up to 6 logs of dynamic range. Get quantitative, reproducible results with Simple Western.</p></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="/instruments/simple-western" class="btn btn-primary" type="link"> Learn About Simple Western </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/webinars/webinar-thumbnail-doseresponse-525x315.jpg" alt="Generating quantitative results like dose-response curves with Simple Western instruments Jess and Peggy Sue" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h3>Table of Contents</h3><ul><li><a href="#Dynamic Range">Broad dynamic range and reproducible results with CVs <20%</a></li><li><a href="#High throughput">High throughput - process 96 samples overnight for strong dose-response curves</a></li><li><a href="#Absolute or relative quantitation with total protein normalization">Absolute or relative quantitation with total protein normalization</a></li></ul><h2><a name="Dynamic Range"></a>Broader Dynamic Range than Western Blots and ELISA</h2><p>Automated Simple Western assays have better detection and protein quantification for Western blot and over a larger range of sample concentration than ELISA.</p></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="/resources/literature/wes-delivers-broader-dynamic-range-compared-traditional-western" class="btn btn-primary" type="link"> Broad Dynamic Range App Note </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/app-protein-quantitation.gif" alt="Protein Quantification Graph" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="High throughput"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2>Strengthen Dose Response Curves</h2> <p>Simple Western is an ideal platform for dose-response measurements in <a href="/resources/testimonials/protac-drug-discovery-jess">drug development studies</a>. Reveal changes in target abundance, target degradation, or post-translational modifications with:</p> <ul> <li>High throughput</li> <li>Speed</li> <li>Sensitivity</li> <li>Accuracy</li> <li>Minimal matrix effects</li> </ul> <p><a href="/instruments/simple-western/simple-western-assays">Simple Western Charge and Size assays</a> deliver accurate dose response measurements in a variety of applications including <a href="/resources/instrument-applications/high-throughput-pharmacokinetic-analysis-with-simple-western">pharmacokinetic assays</a> and<a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-targeted-protein-degradation"> targeted protein degradation</a>.</p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/resources/webinars/simple-western-dose-response" class="btn btn-primary" type="link"> Webinar on Drug Development </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/protac-dose-response-curve-jess.jpg" alt="Better Western blot quantification like PROTAC dose-response curve with Jess" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h3>From Your Peers in Drug Discovery</h3> <p><em>"I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."</em></p> <p>- Rachel Doidge Ph.D, Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK</p></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="/resources/testimonials/protac-drug-discovery-jess" class="btn btn-primary" type="link"> Quantitative PROTAC Studies </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-rachel-doidge.png" alt="Rachel Doidge thumbnail" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Absolute or relative quantitation with total protein normalization"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2>Automated Protein Normalization</h2> <p>Housekeeping controls are unreliable controls for protein normalization. Normalizing target protein expression data by total protein is the only reliable and accurate method to understand how protein expression changes as cells respond to their environment, and scientific journals are increasingly demanding it. Simple Western has several different methods for <a href="/instruments/simple-western/automate-total-protein-normalization-with-simple-western">automated total protein normalization</a> for <a href="/resources/literature/simple-western-produces-publication-ready-results">publication-ready results</a>. <a href="/p/simple-western/jess-automated-western-blot-system_004-650">Jess </a>has a dedicated fluorescence channel for total protein normalization. Just add the <a href="/p/simple-western/protein-normalization-module_dm-pn02">Protein Normalization Reagent </a>and Jess will normalize your protein expression data of targets detected in chemiluminescence and NIR/IR fluorescence channels.</p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/instruments/simple-western/automate-total-protein-normalization-with-simple-western" class="btn btn-primary" type="link"> Protein Normalization Assays </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/jess-superplex-chemiluminescence-fluorescence-graph.png" alt="Jess Superplex Multiplex Immunoassay Chemiluminescence Fluorescence Graph" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2>Automated Western Blot Quantification Analysis</h2> <p>Raw and normalized results automatically appear in Simple Western instrument software <a href="/resources/instrument-software-download-center/compass-software-simple-western">Compass for Simple Western</a>.</p> <ul> <li><a href="/resources/videos/compass-advanced-analysis-module-4-simple-western">Setting up and using a standard curve on Compass</a></li> <li><a href="/resources/videos/compass-advanced-analysis-module-3-simple-western">Protein normalization on Compass</a></li> </ul></div> </div> <div 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Automated Western Blot Quantification with Simple Western | Bio-Techne