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Protein Bioprocessing - Clone Selection | Bio-Techne
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</div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Clone Selection Made Simple</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Getting to the right clone for the highest yield and quality as early as possible may seem difficult – but with our flexible, automated, high-throughput solutions rapid, confident clone selection can be a reality!</p> <p>Take the hassle out of screening, selection, characterization and clone verification – in less time, with no clean up stages and get better data quality than ever before.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Clone-Selection-Cells.png" alt="protein bioprocessing clone selection" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <a href="https://www.bio-techne.com/instruments/single-cell-dispensers" title="Discover Bio-Techne's Single Cell Dispenser Instruments" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/about/v2_AAI-LP-new-Pala.png" alt="Pala Cell Sorter and Single Cell Dispenser hero image" loading="lazy" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h2>Is There An Easy Way to Isolate My Cells?</h2></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Bio-Techne's Single Cell Dispenser technology leverages the powerful combination of microfluidics, flow cytometry, and liquid dispensing to provide rapid, efficient and gentle isolation of single cells. The gentle low-pressure sorting preserves cell viability resulting in higher clonality and better outgrowth, enabling rapid identification of the best clones for <a href="/resources/instrument-applications/antibody-discovery-single-cell-dispenser">antibody discovery</a>.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/instruments/single-cell-dispensers" class="btn btn-primary" type="link"> Discover Bio-Techne's Single Cell Dispenser Instruments </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="Simple-Western"></a>Which Clones to Grow and Which to Leave Behind?</h2> <p>Making this decision early on in the development pipeline will save you time later on… but when characterizing a clone is tedious, requires long hours, and involves a vast array of technologies, screening candidates early-on may not seem like an option.</p> <p>Let <a href="/instruments/simple-western">Simple Western™</a> platforms help you pick the right clone, faster:</p> <ul> <li>Say goodbye to multiple purification steps – even with complex mixtures</li> <li>Low sample volume requirements – cuts down clone propagation time</li> <li>Determine multiple characteristics – confirmation of molecular weight, protein yield and product quality – with just one machine!</li> <li>Get size analysis of proteins from 2 kDa to 440 kDa with picogram-level sensitivity with just 3 μL to 5 μL of starting material.</li> <li>Under time pressure? <a href="/p/simple-western/jess-automated-western-blot-system_004-650">Jess™</a> and <a href="/p/simple-western/abby_004-680">Abby™</a> run 25 samples in 3 hours flat.</li> <li>Lots of clones to screen? <a href="/p/simple-western/sally-sue_004-700">Sally Sue™</a>, <a href="/p/simple-western/peggy-sue_004-800">Peggy Sue™</a>, and the <a href="/p/simple-western/nanopro-1000_004-109">NanoPro™ 1000</a> run 96 samples overnight.</li> </ul></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/jess_004-650" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Jess.png" alt="ProteinSimple instrument Jess for chemiluminescence and fluorescence multiplexing." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/jess_004-650" > <h3 class="card_title_htag"> <div>Jess</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/jess_004-650" > Multiplexed Western Analysis with Jess </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/wes_004-600" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/abby-product-v3.png" alt="ProteinSimple instrument Abby for chemiluminescence assays." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/wes_004-600" > <h3 class="card_title_htag"> <div>Abby</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/wes_004-600" > Automated Western Analysis with Abby </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/sally-sue_004-700" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Sally-Sue.png" alt="ProteinSimple instrument Sally Sue for high-throughput Simple Western size assays." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/sally-sue_004-700" > <h3 class="card_title_htag"> <div>Sally Sue</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/sally-sue_004-700" > Screen 96 Clones Overnight with Sally Sue </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/peggy-sue_004-800" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Peggy-Sue.png" alt="ProteinSimple instrument Peggy Sue for high-throughput charge assays." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/peggy-sue_004-800" > <h3 class="card_title_htag"> <div>Peggy Sue</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/peggy-sue_004-800" > Get Charge and Size Assays with Peggy Sue </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/nanopro-1000_004-109" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-NanoPro.png" alt="ProteinSimple instrument Milo for Single-Cell Westerns." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/nanopro-1000_004-109" > <h3 class="card_title_htag"> <div>NanoPro 1000</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/nanopro-1000_004-109" > Get Charge Assays With NanoPro 1000 </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph-image-banner banner_wrapper"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Clone-Selection-Background-Verify-v3.png" style="background-repeat: no-repeat"> <div class="text_align_left"> <div><h2>Get Simple Western Certified Antibodies from Bio-Techne!</h2></div> <a href="/resources/simple-western-antibody-database" > <button type="button" class="btn btn-primary"> Find Out More </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>Are My Clones Producing High-Quality Therapeutics?</h2> <h4><em>Input so little, get out so much!</em></h4> <p>If there was a super-fast, simple method to analyze the safety, purity and potency of your clones, would you want to know about it?</p> <p>Discover our automated solutions to help you quickly and reliably select a healthy and stable clone:</p> <ul> <li>Peggy Sue, Maurice C., and iCE3 can produce charge heterogeneity profiles with as little as 5 µL and in as little as 10 minutes per sample. </li> <li>Maurice S. accurately confirms purity and heterogeneity of your target protein in just 35 minutes with its robust CE-SDS method.</li> </ul></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/peggy-sue_004-800" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Peggy-Sue.png" alt="ProteinSimple instrument Peggy Sue for high-throughput charge assays." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/peggy-sue_004-800" > <h3 class="card_title_htag"> <div>Peggy Sue</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/peggy-sue_004-800" > High Throughput Westerns with Peggy Sue </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/imaged-capillary-electrophoresis-ice/maurice-s_090-001" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-MauriceS.png" alt="maurice s" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/imaged-capillary-electrophoresis-ice/maurice-s_090-001" > <h3 class="card_title_htag"> <div>Maurice S.</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/p/imaged-capillary-electrophoresis-ice/maurice-s_090-001" > Get CE-SDS data with Maurice S. </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/imaged-capillary-electrophoresis-ice/maurice-c_090-002" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-MauriceC.png" alt="maurice c" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/imaged-capillary-electrophoresis-ice/maurice-c_090-002" > <h3 class="card_title_htag"> <div>Maurice C.</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/p/imaged-capillary-electrophoresis-ice/maurice-c_090-002" > Get cIEF data with Maurice C. </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/imaged-capillary-electrophoresis-ice/ice3_004-001" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-iCE3.png" alt="ice3" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/imaged-capillary-electrophoresis-ice/ice3_004-001" > <h3 class="card_title_htag"> <div>iCE3</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Get cIEF data with iCE3.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/p/imaged-capillary-electrophoresis-ice/ice3_004-001" > Get cIEF data with iCE3 </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Clone-Selection-Milo-Focus-v4.png" alt="protein bioprocessing milo" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h2>Are my Clone Selections Monoclonal?</h2></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Step away from population level resolution of Western blotting and off-target binding of flow cytometry, and embrace the Single-Cell Western revolution.</p> <p>With Single-Cell Westerns on <a href="/instruments/single-cell-western">Milo</a>, you can verify monoclonality of 1,000 single-cells simultaneously.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Home-Bioprocessing-exosome-analysis.png" alt="Exosomes" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h2>Are My Clones Producing The Right Antibody?</h2></div> 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Characterize your clones without disrupting the cell of origin through exosomal analysis.</p> <p><a href="https://www.exosomedx.com/europe/pharma-services/working-us" target="_blank">Expedite your clone selection with Exosome Dx.</a></p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-default"> <div> <a href="/applications/bioprocessing" > <button type="button" class="btn btn-primary"> Back to Protein Bioprocessing home </button> </a> </div> </div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide container-fluid text-white"> <div class="row py-5"> <div class="footer-logo col-xl-3 col-lg-auto d-flex"> <img class="brand-bar-footer-logo" src="/themes/custom/bio_techne_global/logo.svg" alt="Bio-techne logo"> </div> <div class="col-xl-9 col-lg-auto"> <p class="ml-xl-4 bt-px_groteskbold footer-tagline">Global 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