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Secondary True to Life Polyethylene Terephthalate Nanoplastics: Obtention, Characterization, and Hazard Evaluation
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/></div></noscript> <!-- /Yandex.Metrika counter --> <!-- Matomo --> <!-- End Matomo Code --> <title>Secondary True to Life Polyethylene Terephthalate Nanoplastics: Obtention, Characterization, and Hazard Evaluation</title> <meta name="description" content="Secondary True to Life Polyethylene Terephthalate Nanoplastics: Obtention, Characterization, and Hazard Evaluation"> <meta name="keywords" content="nanoplastics, polyethylene terephthalate, physicochemical characterization, cell uptake, cytotoxicity"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link 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href="https://publications.waset.org/abstracts/search?q=Victor%20Fuentes-Cebrian"> Victor Fuentes-Cebrian</a>, <a href="https://publications.waset.org/abstracts/search?q=Oscar%20H.%20Moriones"> Oscar H. Moriones</a>, <a href="https://publications.waset.org/abstracts/search?q=Ricard%20Marcos"> Ricard Marcos</a>, <a href="https://publications.waset.org/abstracts/search?q=Alba%20Hern%C3%A1ndez."> Alba Hern谩ndez.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Micro and nano plastics (MNPLs) are emergent environmental pollutants requiring urgent information on their potential risks to human health. One of the problems associated with the evaluation of their undesirable effects is the lack of real samples matching those resulting from the environmental degradation of plastic wastes. To such end, we propose an easy method to obtain polyethylene terephthalate nano plastics from water plastic bottles (PET-NPLs) but, in principle, applicable to any other plastic goods sources. An extensive characterization indicates that the proposed process produces uniform samples of PET-NPLs of around 100 nm, as determined by using a multi-angle and dynamic light scattering methodology. An important point to be highlighted is that to avoid the metal contamination resulting from methods using metal blades/burrs for milling, trituration, or sanding, we propose to use diamond burrs to produce metal-free samples. To visualize the toxicological profile of the produced PET-NPLs, we have evaluated their ability to be internalized by cells, their cytotoxicity, and their ability to induce oxidative stress and induce DNA damage. In this preliminary approach, we have detected their cellular uptake, but without the induction of significant biological effects. Thus, no relevant increases in toxicity, reactive oxygen species (ROS) induction, or DNA damage -as detected with the comet assay- have been observed. The use of real samples, as produced in this study, will generate relevant data in the discussion about the potential health risks associated with MNPLs exposures. <iframe src="https://publications.waset.org/abstracts/152948.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nanoplastics" title="nanoplastics">nanoplastics</a>, <a href="https://publications.waset.org/abstracts/search?q=polyethylene%20terephthalate" title=" polyethylene terephthalate"> polyethylene terephthalate</a>, <a href="https://publications.waset.org/abstracts/search?q=physicochemical%20characterization" title=" physicochemical characterization"> physicochemical characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20uptake" title=" cell uptake"> cell uptake</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a> </p> <a href="https://publications.waset.org/abstracts/152948/secondary-true-to-life-polyethylene-terephthalate-nanoplastics-obtention-characterization-and-hazard-evaluation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/152948.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">97</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th 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