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/></div><div class="title-container"><h1 class="ds2-5-heading-sans-serif-sm">José Antonio Lupiáñez Cara</h1><div class="affiliations-container fake-truncate js-profile-affiliations"></div></div></div><div class="sidebar-cta-container"><button class="ds2-5-button hidden profile-cta-button grow js-profile-follow-button" data-broccoli-component="user-info.follow-button" data-click-track="profile-user-info-follow-button" data-follow-user-fname="José Antonio" data-follow-user-id="15266903" data-follow-user-source="profile_button" data-has-google="false"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">add</span>Follow</button><button class="ds2-5-button hidden profile-cta-button grow js-profile-unfollow-button" data-broccoli-component="user-info.unfollow-button" data-click-track="profile-user-info-unfollow-button" data-unfollow-user-id="15266903"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">done</span>Following</button></div></div><div class="user-stats-container"><a><div class="stat-container js-profile-followers"><p class="label">Followers</p><p class="data">13</p></div></a><a><div class="stat-container js-profile-followees" data-broccoli-component="user-info.followees-count" data-click-track="profile-expand-user-info-following"><p class="label">Following</p><p class="data">11</p></div></a><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div><div class="user-bio-container"><div class="profile-bio fake-truncate js-profile-about" style="margin: 0px;"><b>Address: </b>Granada, Andalucia, Spain<br /><div class="js-profile-less-about u-linkUnstyled u-tcGrayDarker u-textDecorationUnderline u-displayNone">less</div></div></div><div class="suggested-academics-container"><div class="suggested-academics--header"><p 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id="Papers"><h3 class="profile--tab_heading_container">Papers by José Antonio Lupiáñez Cara</h3></div><div class="js-work-strip profile--work_container" data-work-id="94793714"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94793714/Efficient_In_Vitro_and_In_Vivo_Anti_Inflammatory_Activity_of_a_Diamine_PEGylated_Oleanolic_Acid_Derivative"><img alt="Research paper thumbnail of Efficient In Vitro and In Vivo Anti-Inflammatory Activity of a Diamine-PEGylated Oleanolic Acid Derivative" class="work-thumbnail" src="https://attachments.academia-assets.com/97153357/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94793714/Efficient_In_Vitro_and_In_Vivo_Anti_Inflammatory_Activity_of_a_Diamine_PEGylated_Oleanolic_Acid_Derivative">Efficient In Vitro and In Vivo Anti-Inflammatory Activity of a Diamine-PEGylated Oleanolic Acid Derivative</a></div><div class="wp-workCard_item"><span>International Journal of Molecular Sciences</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recent evidence has shown that inflammation can contribute to all tumorigenic states. We have inv...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recent evidence has shown that inflammation can contribute to all tumorigenic states. We have investigated the anti-inflammatory effects of a diamine-PEGylated derivative of oleanolic acid (OADP), in vitro and in vivo with inflammation models. In addition, we have determined the sub-cytotoxic concentrations for anti-inflammatory assays of OADP in RAW 264.7 cells. The inflammatory process began with incubation with lipopolysaccharide (LPS). Nitric oxide production levels were also determined, exceeding 75% inhibition of NO for a concentration of 1 µg/mL of OADP. Cell-cycle analysis showed a reversal of the arrest in the G0/G1 phase in LPS-stimulated RAW 264.7 cells. Furthermore, through Western blot analysis, we have determined the probable molecular mechanism activated by OADP; the inhibition of the expression of cytokines such as TNF-α, IL-1β, iNOS, and COX-2; and the blocking of p-IκBα production in LPS-stimulated RAW 264.7 cells. Finally, we have analyzed the anti-inflammatory ac...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="57432f0d6f73244dafd33bc8ebfc5340" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":97153357,"asset_id":94793714,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/97153357/download_file?st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94793714"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94793714"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94793714; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94793714]").text(description); $(".js-view-count[data-work-id=94793714]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94793714; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94793714']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94793714, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "57432f0d6f73244dafd33bc8ebfc5340" } } $('.js-work-strip[data-work-id=94793714]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94793714,"title":"Efficient In Vitro and In Vivo Anti-Inflammatory Activity of a Diamine-PEGylated Oleanolic Acid Derivative","translated_title":"","metadata":{"abstract":"Recent evidence has shown that inflammation can contribute to all tumorigenic states. We have investigated the anti-inflammatory effects of a diamine-PEGylated derivative of oleanolic acid (OADP), in vitro and in vivo with inflammation models. In addition, we have determined the sub-cytotoxic concentrations for anti-inflammatory assays of OADP in RAW 264.7 cells. The inflammatory process began with incubation with lipopolysaccharide (LPS). Nitric oxide production levels were also determined, exceeding 75% inhibition of NO for a concentration of 1 µg/mL of OADP. Cell-cycle analysis showed a reversal of the arrest in the G0/G1 phase in LPS-stimulated RAW 264.7 cells. Furthermore, through Western blot analysis, we have determined the probable molecular mechanism activated by OADP; the inhibition of the expression of cytokines such as TNF-α, IL-1β, iNOS, and COX-2; and the blocking of p-IκBα production in LPS-stimulated RAW 264.7 cells. 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Furthermore, through Western blot analysis, we have determined the probable molecular mechanism activated by OADP; the inhibition of the expression of cytokines such as TNF-α, IL-1β, iNOS, and COX-2; and the blocking of p-IκBα production in LPS-stimulated RAW 264.7 cells. 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We have investigated the anti-inflammatory effects of a diamine-PEGylated derivative of oleanolic acid (OADP), in vitro and in vivo with inflammation models. In addition, we have determined the sub-cytotoxic concentrations for anti-inflammatory assays of OADP in RAW 264.7 cells. The inflammatory process began with incubation with lipopolysaccharide (LPS). Nitric oxide production levels were also determined, exceeding 75% inhibition of NO for a concentration of 1 µg/mL of OADP. Cell-cycle analysis showed a reversal of the arrest in the G0/G1 phase in LPS-stimulated RAW 264.7 cells. Furthermore, through Western blot analysis, we have determined the probable molecular mechanism activated by OADP; the inhibition of the expression of cytokines such as TNF-α, IL-1β, iNOS, and COX-2; and the blocking of p-IκBα production in LPS-stimulated RAW 264.7 cells. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94793712"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94793712/A_New_HPLC_MS_Method_for_Measuring_Maslinic_Acid_and_Oleanolic_Acid_in_HT29_and_HepG2_Human_Cancer_Cells"><img alt="Research paper thumbnail of A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/97153378/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94793712/A_New_HPLC_MS_Method_for_Measuring_Maslinic_Acid_and_Oleanolic_Acid_in_HT29_and_HepG2_Human_Cancer_Cells">A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells</a></div><div class="wp-workCard_item"><span>International Journal of Molecular Sciences</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have imp...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bdbbdaae87b4d652f515be08fbcccb39" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":97153378,"asset_id":94793712,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/97153378/download_file?st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94793712"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94793712"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94793712; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94793712]").text(description); $(".js-view-count[data-work-id=94793712]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94793712; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94793712']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94793712, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bdbbdaae87b4d652f515be08fbcccb39" } } $('.js-work-strip[data-work-id=94793712]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94793712,"title":"A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells","translated_title":"","metadata":{"publisher":"MDPI AG","grobid_abstract":"Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. 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The anticarcinogen activity has been assayed against three cancer cell lines: HT29, human colon cancer cells; Hep-G2, human hepatic cells; and B16-F10, murine melanoma cells. First, we determined the cytotoxicity of the different compounds, finding that the most effective compound was compound 8c against all cell lines and both compounds 4 and 6 in human Hep-G2 and HT29 cell lines. Compounds 4 and 8c were selected for the percentage of apoptosis determination, cell cycle distribution, and mitochondrial membrane potential measure because these products presented the lowest IC50 values in two of the three cancer cell lines assayed (B16-F10 and HepG2), and were two of the three products with lowest IC50 in HT29 cell line. Moreover, the percentages of apoptosis induction were determined for compounds 4 and 8c, showing that the highest values we...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4a02842e46ab44d20265db9d25b8b903" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":97153341,"asset_id":94793691,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/97153341/download_file?st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94793691"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94793691"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94793691; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94793691]").text(description); $(".js-view-count[data-work-id=94793691]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94793691; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94793691']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94793691, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4a02842e46ab44d20265db9d25b8b903" } } $('.js-work-strip[data-work-id=94793691]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94793691,"title":"Diclofenac N-Derivatives as Therapeutic Agents with Anti-Inflammatory and Anti-Cancer Effect","translated_title":"","metadata":{"abstract":"A series of diclofenac N-derivatives (2, 4, 6, 8c, 9c, 10a-c) were synthesized in order to test their anti-cancer and anti-inflammatory effects. 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class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87786071/The_oleanolic_acid_derivative_3_O_succinyl_28_O_benzyl_oleanolate_induces_apoptosis_in_B16_F10_melanoma_cells_via_the_mitochondrial_apoptotic_pathway"><img alt="Research paper thumbnail of The oleanolic acid derivative, 3-O-succinyl-28-O-benzyl oleanolate, induces apoptosis in B16–F10 melanoma cells via the mitochondrial apoptotic pathway" class="work-thumbnail" src="https://attachments.academia-assets.com/91904495/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87786071/The_oleanolic_acid_derivative_3_O_succinyl_28_O_benzyl_oleanolate_induces_apoptosis_in_B16_F10_melanoma_cells_via_the_mitochondrial_apoptotic_pathway">The oleanolic acid derivative, 3-O-succinyl-28-O-benzyl oleanolate, induces apoptosis in B16–F10 melanoma cells via the mitochondrial apoptotic pathway</a></div><div class="wp-workCard_item"><span>RSC Advances</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antiproliferative and proapoptotic effects of 3-O-succinyl-28-O-benzyl oleanolate on B16–F10 skin...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antiproliferative and proapoptotic effects of 3-O-succinyl-28-O-benzyl oleanolate on B16–F10 skin-melanoma cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="79969724fcf70bd7a23c333bf535dfeb" class="wp-workCard--action" rel="nofollow" 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Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/91904549/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87786070/Maslinic_Acid_a_Natural_Triterpene_Induces_a_Death_Receptor_Mediated_Apoptotic_Mechanism_in_Caco_2_p53_Deficient_Colon_Adenocarcinoma_Cells">Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells</a></div><div class="wp-workCard_item"><span>PLOS ONE</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of oli...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dosedependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases-8 and-3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cadee59bee13df2feae9d1c8c9c3e19d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":91904549,"asset_id":87786070,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/91904549/download_file?st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87786070"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87786070"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87786070; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87786070]").text(description); $(".js-view-count[data-work-id=87786070]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87786070; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87786070']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87786070, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cadee59bee13df2feae9d1c8c9c3e19d" } } $('.js-work-strip[data-work-id=87786070]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87786070,"title":"Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells","translated_title":"","metadata":{"publisher":"Public Library of Science (PLoS)","grobid_abstract":"Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dosedependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases-8 and-3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"PLOS ONE","grobid_abstract_attachment_id":91904549},"translated_abstract":null,"internal_url":"https://www.academia.edu/87786070/Maslinic_Acid_a_Natural_Triterpene_Induces_a_Death_Receptor_Mediated_Apoptotic_Mechanism_in_Caco_2_p53_Deficient_Colon_Adenocarcinoma_Cells","translated_internal_url":"","created_at":"2022-10-03T02:11:54.139-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":91904549,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91904549/thumbnails/1.jpg","file_name":"7c3b9af457401ff32cf5a8e80aaa905e2c6a.pdf","download_url":"https://www.academia.edu/attachments/91904549/download_file?st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_a_Natural_Triterpene_Induc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91904549/7c3b9af457401ff32cf5a8e80aaa905e2c6a-libre.pdf?1664788745=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_a_Natural_Triterpene_Induc.pdf\u0026Expires=1736936358\u0026Signature=ECJX10-53IgSbGcVUnlrycBsouHazwsmvoklKgdNvxCCoSuqj10oaVH3iBgJs6NILERGlkCs3eY1DtVHHf5DY9kzJJ~ZfmR5GBETPSO96TrbG14Wul4nXc8a2dFP7pX6lqT789Dd4BQVwk-cE~pq~CgXKBQrohvQnKJ47o53qH7RGL8vKXtB381p1IDm0ZoHPJ1te8UAU9sEny0rglQDjt-EjJCetZjqk32Pn~gOxux8SaONhKmO5WyjltCQ5RRuJrGzyBFRjznwk2NUU5ml8rEtOglB286ummpM4Ft-XejcG5TyNk-7tm5xSUlziWcnvIphdVqip~5x6yDPfn8wEw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Maslinic_Acid_a_Natural_Triterpene_Induces_a_Death_Receptor_Mediated_Apoptotic_Mechanism_in_Caco_2_p53_Deficient_Colon_Adenocarcinoma_Cells","translated_slug":"","page_count":16,"language":"en","content_type":"Work","summary":"Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dosedependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases-8 and-3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":91904549,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91904549/thumbnails/1.jpg","file_name":"7c3b9af457401ff32cf5a8e80aaa905e2c6a.pdf","download_url":"https://www.academia.edu/attachments/91904549/download_file?st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_a_Natural_Triterpene_Induc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91904549/7c3b9af457401ff32cf5a8e80aaa905e2c6a-libre.pdf?1664788745=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_a_Natural_Triterpene_Induc.pdf\u0026Expires=1736936358\u0026Signature=ECJX10-53IgSbGcVUnlrycBsouHazwsmvoklKgdNvxCCoSuqj10oaVH3iBgJs6NILERGlkCs3eY1DtVHHf5DY9kzJJ~ZfmR5GBETPSO96TrbG14Wul4nXc8a2dFP7pX6lqT789Dd4BQVwk-cE~pq~CgXKBQrohvQnKJ47o53qH7RGL8vKXtB381p1IDm0ZoHPJ1te8UAU9sEny0rglQDjt-EjJCetZjqk32Pn~gOxux8SaONhKmO5WyjltCQ5RRuJrGzyBFRjznwk2NUU5ml8rEtOglB286ummpM4Ft-XejcG5TyNk-7tm5xSUlziWcnvIphdVqip~5x6yDPfn8wEw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry"},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome"},{"id":15719,"name":"Mitochondria","url":"https://www.academia.edu/Documents/in/Mitochondria"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation"},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation"},{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one"},{"id":247477,"name":"Caspase","url":"https://www.academia.edu/Documents/in/Caspase"},{"id":314125,"name":"Caco-2 cells","url":"https://www.academia.edu/Documents/in/Caco-2_cells"},{"id":501806,"name":"Protein Expression","url":"https://www.academia.edu/Documents/in/Protein_Expression"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"},{"id":1692059,"name":"Minimum inhibitory concentration","url":"https://www.academia.edu/Documents/in/Minimum_inhibitory_concentration"},{"id":1745478,"name":"Adenocarcinoma","url":"https://www.academia.edu/Documents/in/Adenocarcinoma"},{"id":1758650,"name":"Triterpenes","url":"https://www.academia.edu/Documents/in/Triterpenes"},{"id":3793278,"name":"Colonic Neoplasms","url":"https://www.academia.edu/Documents/in/Colonic_Neoplasms"}],"urls":[{"id":24416062,"url":"http://dx.plos.org/10.1371/journal.pone.0146178"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="87786051"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87786051/Maslinic_Acid_Enriched_Diet_Decreases_Intestinal_Tumorigenesis_in_ApcMin_Mice_through_Transcriptomic_and_Metabolomic_Reprogramming"><img alt="Research paper thumbnail of Maslinic Acid-Enriched Diet Decreases Intestinal Tumorigenesis in ApcMin/+ Mice through Transcriptomic and Metabolomic Reprogramming" class="work-thumbnail" src="https://attachments.academia-assets.com/91904531/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87786051/Maslinic_Acid_Enriched_Diet_Decreases_Intestinal_Tumorigenesis_in_ApcMin_Mice_through_Transcriptomic_and_Metabolomic_Reprogramming">Maslinic Acid-Enriched Diet Decreases Intestinal Tumorigenesis in ApcMin/+ Mice through Transcriptomic and Metabolomic Reprogramming</a></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Chemoprevention is a pragmatic approach to reduce the risk of colorectal cancer, one of the leadi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Chemoprevention is a pragmatic approach to reduce the risk of colorectal cancer, one of the leading causes of cancerrelated death in western countries. In this regard, maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, is known to inhibit proliferation and induce apoptosis in colon cancer cell lines without affecting normal intestinal cells. The present study evaluated the chemopreventive efficacy and associated mechanisms of maslinic acid treatment on spontaneous intestinal tumorigenesis in Apc Min/+ mice. Twenty-two mice were randomized into 2 groups: control group and MA group, fed with a maslinic acid-supplemented diet for six weeks. MA treatment reduced total intestinal polyp formation by 45% (P,0.01). Putative molecular mechanisms associated with suppressing intestinal polyposis in Apc Min/+ mice were investigated by comparing microarray expression profiles of MA-treated and control mice and by analyzing the serum metabolic profile using NMR techniques. The different expression phenotype induced by MA suggested that it exerts its chemopreventive action mainly by inhibiting cell-survival signaling and inflammation. These changes eventually induce G1-phase cell cycle arrest and apoptosis. Moreover, the metabolic changes induced by MA treatment were associated with a protective profile against intestinal tumorigenesis. These results show the efficacy and underlying mechanisms of MA against intestinal tumor development in the Apc Min/+ mice model, suggesting its chemopreventive potential against colorectal cancer.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2efdda220922496498d0e670b57a5775" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":91904531,"asset_id":87786051,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/91904531/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87786051"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87786051"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87786051; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87786051]").text(description); $(".js-view-count[data-work-id=87786051]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87786051; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87786051']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87786051, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2efdda220922496498d0e670b57a5775" } } $('.js-work-strip[data-work-id=87786051]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87786051,"title":"Maslinic Acid-Enriched Diet Decreases Intestinal Tumorigenesis in ApcMin/+ Mice through Transcriptomic and Metabolomic Reprogramming","translated_title":"","metadata":{"publisher":"Public Library of Science (PLoS)","grobid_abstract":"Chemoprevention is a pragmatic approach to reduce the risk of colorectal cancer, one of the leading causes of cancerrelated death in western countries. 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In this regard, maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, is known to inhibit proliferation and induce apoptosis in colon cancer cell lines without affecting normal intestinal cells. The present study evaluated the chemopreventive efficacy and associated mechanisms of maslinic acid treatment on spontaneous intestinal tumorigenesis in Apc Min/+ mice. Twenty-two mice were randomized into 2 groups: control group and MA group, fed with a maslinic acid-supplemented diet for six weeks. MA treatment reduced total intestinal polyp formation by 45% (P,0.01). Putative molecular mechanisms associated with suppressing intestinal polyposis in Apc Min/+ mice were investigated by comparing microarray expression profiles of MA-treated and control mice and by analyzing the serum metabolic profile using NMR techniques. The different expression phenotype induced by MA suggested that it exerts its chemopreventive action mainly by inhibiting cell-survival signaling and inflammation. These changes eventually induce G1-phase cell cycle arrest and apoptosis. Moreover, the metabolic changes induced by MA treatment were associated with a protective profile against intestinal tumorigenesis. These results show the efficacy and underlying mechanisms of MA against intestinal tumor development in the Apc Min/+ mice model, suggesting its chemopreventive potential against colorectal cancer.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":91904531,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91904531/thumbnails/1.jpg","file_name":"75e5cc0cf301a9482d1a0f2eb075f10b90f1.pdf","download_url":"https://www.academia.edu/attachments/91904531/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_Enriched_Diet_Decreases_In.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91904531/75e5cc0cf301a9482d1a0f2eb075f10b90f1-libre.pdf?1664788748=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_Enriched_Diet_Decreases_In.pdf\u0026Expires=1736936358\u0026Signature=JPR3HAhmx07eMUdqd~VlYZath~YLFseA5QNCGwNhFMMyqbamgQyvdCDzK5SZk4p~qohTKPdOGcjC1FlYjcL7XKP0Ld4QOeofYElzpCL~4SoJY3sgCoFkD6resMFCytH9ACgiLGxLQJ~86ZuXOqyc2cdiNX4VLC8GhOP6-h-opnePj0XzIZq9cwYaA4ykPgbLFsvc26A56u7MqSXyxzMyyrHnwkDbZkmsyPMnaIX43EX7ip5XZzkjrLeWbBYC9Ha6Bsr1dNMh6CkGqG3n4P5UREXaRe8J2sqhUY93OgyzqmuFnYn9s9zCUYv04lntPbO9jF9SkWtV4UdOQkhzyPXn0g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3770,"name":"Metabolism","url":"https://www.academia.edu/Documents/in/Metabolism"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":37292,"name":"Colorectal cancer","url":"https://www.academia.edu/Documents/in/Colorectal_cancer"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":136837,"name":"Tumors","url":"https://www.academia.edu/Documents/in/Tumors"},{"id":152562,"name":"Dietary Supplements","url":"https://www.academia.edu/Documents/in/Dietary_Supplements"},{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one"},{"id":375301,"name":"Microarray Analysis","url":"https://www.academia.edu/Documents/in/Microarray_Analysis"},{"id":1198158,"name":"Metabolisme","url":"https://www.academia.edu/Documents/in/Metabolisme"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"},{"id":1758650,"name":"Triterpenes","url":"https://www.academia.edu/Documents/in/Triterpenes"},{"id":1810445,"name":"Gene expression profiling","url":"https://www.academia.edu/Documents/in/Gene_expression_profiling"},{"id":2940273,"name":"real time polymerase chain reaction","url":"https://www.academia.edu/Documents/in/real_time_polymerase_chain_reaction"},{"id":3067128,"name":"reverse transcriptase polymerase chain reaction","url":"https://www.academia.edu/Documents/in/reverse_transcriptase_polymerase_chain_reaction"}],"urls":[{"id":24416053,"url":"http://dx.plos.org/10.1371/journal.pone.0059392"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="86649934"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/86649934/Unveiling_the_Differential_Anti_Oxidant_Activity_of_Maslinic_Acid_in_Murine_Melanoma_Cells_and_in_Rat_Embryonic_Healthy_Cells_Following_Treatment_With_Hydrogen_Peroxide"><img alt="Research paper thumbnail of Unveiling the Differential Anti-Oxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment With Hydrogen Peroxide" class="work-thumbnail" src="https://attachments.academia-assets.com/91052747/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/86649934/Unveiling_the_Differential_Anti_Oxidant_Activity_of_Maslinic_Acid_in_Murine_Melanoma_Cells_and_in_Rat_Embryonic_Healthy_Cells_Following_Treatment_With_Hydrogen_Peroxide">Unveiling the Differential Anti-Oxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment With Hydrogen Peroxide</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions hav...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM) whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM of H2O2 were added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, being higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in anti-oxidant enzymes activities depending on cell line. In A10 cells, all enzymes were up-regulated, but in B16F10 cells only superoxide dismutase, glutathione S-transferase ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1650216311fd560848108e317cff3fbf" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":91052747,"asset_id":86649934,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/91052747/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="86649934"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="86649934"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 86649934; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=86649934]").text(description); $(".js-view-count[data-work-id=86649934]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 86649934; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='86649934']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 86649934, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1650216311fd560848108e317cff3fbf" } } $('.js-work-strip[data-work-id=86649934]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":86649934,"title":"Unveiling the Differential Anti-Oxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment With Hydrogen Peroxide","translated_title":"","metadata":{"abstract":"Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM) whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM of H2O2 were added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, being higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in anti-oxidant enzymes activities depending on cell line. In A10 cells, all enzymes were up-regulated, but in B16F10 cells only superoxide dismutase, glutathione S-transferase ...","publisher":"MDPI AG","publication_date":{"day":null,"month":null,"year":2020,"errors":{}}},"translated_abstract":"Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM) whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM of H2O2 were added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, being higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in anti-oxidant enzymes activities depending on cell line. In A10 cells, all enzymes were up-regulated, but in B16F10 cells only superoxide dismutase, glutathione S-transferase ...","internal_url":"https://www.academia.edu/86649934/Unveiling_the_Differential_Anti_Oxidant_Activity_of_Maslinic_Acid_in_Murine_Melanoma_Cells_and_in_Rat_Embryonic_Healthy_Cells_Following_Treatment_With_Hydrogen_Peroxide","translated_internal_url":"","created_at":"2022-09-14T10:42:04.040-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":91052747,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91052747/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/91052747/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Unveiling_the_Differential_Anti_Oxidant.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91052747/pdf-libre.pdf?1663177490=\u0026response-content-disposition=attachment%3B+filename%3DUnveiling_the_Differential_Anti_Oxidant.pdf\u0026Expires=1736936359\u0026Signature=TxDa04QaCQL9WvXFriMfACnWEjvxmVou6TaktEJgh66Q9ZeKPQIFhnQwyDVbrYGsFM5AiPxBV6um5F5qLNf-x0sBD3Vo4-5cdO9Q6xCcSanXydCXxB4rghBJjfp-KmtnbhmADuDUvNpjkXq7sxJba8-Onoizo-bRd3QN~0VmKPBjBd-cAeM6sv3C4ENNCDgJwfNU29N7IH05OJj1vHl2~2RH8nFHg3MLgcqzqoX3X9kb4v~tcORLzdgc0gUcfVyaAaOswwelgMjJVgmXRvWYvBAz~Q9rWrvx2RRZG2Oq2kIslcEidv5TWhxNgUyzkvfbUTRPm5x-rLCAQqLp5sEHgg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Unveiling_the_Differential_Anti_Oxidant_Activity_of_Maslinic_Acid_in_Murine_Melanoma_Cells_and_in_Rat_Embryonic_Healthy_Cells_Following_Treatment_With_Hydrogen_Peroxide","translated_slug":"","page_count":14,"language":"en","content_type":"Work","summary":"Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM) whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM of H2O2 were added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, being higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in anti-oxidant enzymes activities depending on cell line. In A10 cells, all enzymes were up-regulated, but in B16F10 cells only superoxide dismutase, glutathione S-transferase ...","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":91052747,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91052747/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/91052747/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Unveiling_the_Differential_Anti_Oxidant.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91052747/pdf-libre.pdf?1663177490=\u0026response-content-disposition=attachment%3B+filename%3DUnveiling_the_Differential_Anti_Oxidant.pdf\u0026Expires=1736936359\u0026Signature=TxDa04QaCQL9WvXFriMfACnWEjvxmVou6TaktEJgh66Q9ZeKPQIFhnQwyDVbrYGsFM5AiPxBV6um5F5qLNf-x0sBD3Vo4-5cdO9Q6xCcSanXydCXxB4rghBJjfp-KmtnbhmADuDUvNpjkXq7sxJba8-Onoizo-bRd3QN~0VmKPBjBd-cAeM6sv3C4ENNCDgJwfNU29N7IH05OJj1vHl2~2RH8nFHg3MLgcqzqoX3X9kb4v~tcORLzdgc0gUcfVyaAaOswwelgMjJVgmXRvWYvBAz~Q9rWrvx2RRZG2Oq2kIslcEidv5TWhxNgUyzkvfbUTRPm5x-rLCAQqLp5sEHgg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":531,"name":"Organic Chemistry","url":"https://www.academia.edu/Documents/in/Organic_Chemistry"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":290433,"name":"Antioxidant Activity","url":"https://www.academia.edu/Documents/in/Antioxidant_Activity"},{"id":328449,"name":"Molecules","url":"https://www.academia.edu/Documents/in/Molecules"},{"id":842299,"name":"Antioxidant enzymes","url":"https://www.academia.edu/Documents/in/Antioxidant_enzymes"},{"id":1485949,"name":"Preprints","url":"https://www.academia.edu/Documents/in/Preprints"},{"id":2284202,"name":"Anti-proliferative activity","url":"https://www.academia.edu/Documents/in/Anti-proliferative_activity"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="82718670"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/82718670/Maslinic_Acid_a_Triterpene_from_Olive_Affects_the_Antioxidant_and_Mitochondrial_Status_of_B16F10_Melanoma_Cells_Grown_under_Stressful_Conditions"><img alt="Research paper thumbnail of Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions" class="work-thumbnail" src="https://attachments.academia-assets.com/88333635/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/82718670/Maslinic_Acid_a_Triterpene_from_Olive_Affects_the_Antioxidant_and_Mitochondrial_Status_of_B16F10_Melanoma_Cells_Grown_under_Stressful_Conditions">Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions</a></div><div class="wp-workCard_item"><span>Evidence-Based Complementary and Alternative Medicine</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene....</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50values of MA. The DHR 123 data sh...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a2cc34971c865d9b932ddd0c9a669476" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88333635,"asset_id":82718670,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88333635/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="82718670"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="82718670"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 82718670; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=82718670]").text(description); $(".js-view-count[data-work-id=82718670]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 82718670; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='82718670']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 82718670, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a2cc34971c865d9b932ddd0c9a669476" } } $('.js-work-strip[data-work-id=82718670]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":82718670,"title":"Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions","translated_title":"","metadata":{"abstract":"Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50values of MA. The DHR 123 data sh...","publisher":"Hindawi Limited","publication_date":{"day":null,"month":null,"year":2015,"errors":{}},"publication_name":"Evidence-Based Complementary and Alternative Medicine"},"translated_abstract":"Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50values of MA. The DHR 123 data sh...","internal_url":"https://www.academia.edu/82718670/Maslinic_Acid_a_Triterpene_from_Olive_Affects_the_Antioxidant_and_Mitochondrial_Status_of_B16F10_Melanoma_Cells_Grown_under_Stressful_Conditions","translated_internal_url":"","created_at":"2022-07-06T12:52:59.784-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88333635,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88333635/thumbnails/1.jpg","file_name":"272457.pdf","download_url":"https://www.academia.edu/attachments/88333635/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_a_Triterpene_from_Olive_Af.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88333635/272457-libre.pdf?1657137749=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_a_Triterpene_from_Olive_Af.pdf\u0026Expires=1736936359\u0026Signature=WBg6i9LpgbWimWW3jvedkqdJyFhHB6Nfr-wPJICoE46W9KfGEVVAG2bryhAPzvFET7k0GZS91F5aBBpAaWJj7P-QxgEBG6Kk5asmGaognvIG~pmm92JF1zSdZmS0jw6Uecbh0NMjrL90dmUOzOqg-DBix~if1gjF82O1z5dJ0bzdYe8VApSgcuRe-Ai9w4U3lIbx25hR-ZH~G2NjNFzxrbf~6iRdoRfzKmeBs6EaJC6TjEqn-tJcmE1KD8gZRpQ~5wJEc3I3qKNjEL5ztothIq-wYH29xP7DqAZuYp95vPr6wpY3jDwmROmF70JTGbRudLwz60Bmdb9KjflncsLZiA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Maslinic_Acid_a_Triterpene_from_Olive_Affects_the_Antioxidant_and_Mitochondrial_Status_of_B16F10_Melanoma_Cells_Grown_under_Stressful_Conditions","translated_slug":"","page_count":12,"language":"en","content_type":"Work","summary":"Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50values of MA. The DHR 123 data sh...","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":88333635,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88333635/thumbnails/1.jpg","file_name":"272457.pdf","download_url":"https://www.academia.edu/attachments/88333635/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_a_Triterpene_from_Olive_Af.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88333635/272457-libre.pdf?1657137749=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_a_Triterpene_from_Olive_Af.pdf\u0026Expires=1736936359\u0026Signature=WBg6i9LpgbWimWW3jvedkqdJyFhHB6Nfr-wPJICoE46W9KfGEVVAG2bryhAPzvFET7k0GZS91F5aBBpAaWJj7P-QxgEBG6Kk5asmGaognvIG~pmm92JF1zSdZmS0jw6Uecbh0NMjrL90dmUOzOqg-DBix~if1gjF82O1z5dJ0bzdYe8VApSgcuRe-Ai9w4U3lIbx25hR-ZH~G2NjNFzxrbf~6iRdoRfzKmeBs6EaJC6TjEqn-tJcmE1KD8gZRpQ~5wJEc3I3qKNjEL5ztothIq-wYH29xP7DqAZuYp95vPr6wpY3jDwmROmF70JTGbRudLwz60Bmdb9KjflncsLZiA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":54178,"name":"Olive","url":"https://www.academia.edu/Documents/in/Olive"},{"id":103339,"name":"Antioxidant","url":"https://www.academia.edu/Documents/in/Antioxidant"},{"id":1029006,"name":"Reactive Oxygen Species (ROS)","url":"https://www.academia.edu/Documents/in/Reactive_Oxygen_Species_ROS_"},{"id":1407259,"name":"Non-Melanoma Skin Cancer","url":"https://www.academia.edu/Documents/in/Non-Melanoma_Skin_Cancer"}],"urls":[{"id":21974509,"url":"http://downloads.hindawi.com/journals/ecam/2015/272457.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77328102"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/77328102/Influencia_de_las_alteraciones_de_pH_en_los_medios_de_cultivo_para_la_obtenci%C3%B3n_de_formas_metac%C3%ADclicas_de_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Influencia de las alteraciones de pH en los medios de cultivo para la obtención de formas metacíclicas de Trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/77328102/Influencia_de_las_alteraciones_de_pH_en_los_medios_de_cultivo_para_la_obtenci%C3%B3n_de_formas_metac%C3%ADclicas_de_Trypanosoma_cruzi">Influencia de las alteraciones de pH en los medios de cultivo para la obtención de formas metacíclicas de Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The influence of several modifications of Grace and MEM (minimal essential medium) media on growt...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The influence of several modifications of Grace and MEM (minimal essential medium) media on growth of Trypanosoma cruzi epimastigote forms and differentiation to metacyclic infective forms is studied. The modifications induced by pH shifts, rise growth ( 1.3-fold in Grace&amp;#39;s medium and 1.4-fold in MEM medium) and metacyclic forms percentage (3.9- and 5.9-fold, respectively). Possible influence of chemical and physico-chemical factors in these increases is proposed and discussed.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77328102"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77328102"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77328102; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77328102]").text(description); $(".js-view-count[data-work-id=77328102]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77328102; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77328102']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77328102, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77328102]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77328102,"title":"Influencia de las alteraciones de pH en los medios de cultivo para la obtención de formas metacíclicas de Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"The influence of several modifications of Grace and MEM (minimal essential medium) media on growth of Trypanosoma cruzi epimastigote forms and differentiation to metacyclic infective forms is studied. The modifications induced by pH shifts, rise growth ( 1.3-fold in Grace\u0026amp;#39;s medium and 1.4-fold in MEM medium) and metacyclic forms percentage (3.9- and 5.9-fold, respectively). Possible influence of chemical and physico-chemical factors in these increases is proposed and discussed.","publication_date":{"day":null,"month":null,"year":1987,"errors":{}}},"translated_abstract":"The influence of several modifications of Grace and MEM (minimal essential medium) media on growth of Trypanosoma cruzi epimastigote forms and differentiation to metacyclic infective forms is studied. The modifications induced by pH shifts, rise growth ( 1.3-fold in Grace\u0026amp;#39;s medium and 1.4-fold in MEM medium) and metacyclic forms percentage (3.9- and 5.9-fold, respectively). Possible influence of chemical and physico-chemical factors in these increases is proposed and discussed.","internal_url":"https://www.academia.edu/77328102/Influencia_de_las_alteraciones_de_pH_en_los_medios_de_cultivo_para_la_obtenci%C3%B3n_de_formas_metac%C3%ADclicas_de_Trypanosoma_cruzi","translated_internal_url":"","created_at":"2022-04-23T00:26:34.388-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Influencia_de_las_alteraciones_de_pH_en_los_medios_de_cultivo_para_la_obtención_de_formas_metacíclicas_de_Trypanosoma_cruzi","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The influence of several modifications of Grace and MEM (minimal essential medium) media on growth of Trypanosoma cruzi epimastigote forms and differentiation to metacyclic infective forms is studied. The modifications induced by pH shifts, rise growth ( 1.3-fold in Grace\u0026amp;#39;s medium and 1.4-fold in MEM medium) and metacyclic forms percentage (3.9- and 5.9-fold, respectively). Possible influence of chemical and physico-chemical factors in these increases is proposed and discussed.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":2477473,"name":"Metacyclogenesis","url":"https://www.academia.edu/Documents/in/Metacyclogenesis"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77328034"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77328034/A_Diamine_PEGylated_Oleanolic_Acid_Derivative_Induced_Efficient_Apoptosis_through_a_Death_Receptor_and_Mitochondrial_Apoptotic_Pathway_in_HepG2_Human_Hepatoma_Cells"><img alt="Research paper thumbnail of A Diamine-PEGylated Oleanolic Acid Derivative Induced Efficient Apoptosis through a Death Receptor and Mitochondrial Apoptotic Pathway in HepG2 Human Hepatoma Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/84719293/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77328034/A_Diamine_PEGylated_Oleanolic_Acid_Derivative_Induced_Efficient_Apoptosis_through_a_Death_Receptor_and_Mitochondrial_Apoptotic_Pathway_in_HepG2_Human_Hepatoma_Cells">A Diamine-PEGylated Oleanolic Acid Derivative Induced Efficient Apoptosis through a Death Receptor and Mitochondrial Apoptotic Pathway in HepG2 Human Hepatoma Cells</a></div><div class="wp-workCard_item"><span>Biomolecules</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have s...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have shown that the diamine-(PEG)ylated oleanolic acid (OADP) has strong anti-tumor effects in HCCs. In this study, we evaluated the anti-tumor mechanisms of OADP in the HepG2 HCC cell line. The cytotoxicity results showed that HepG2 cell viability was markedly reduced, with a very low 50% of cell growth inhibitory concentration (IC50, 0.14 µg/mL). We then investigated the anti-tumor mechanisms of OADP in HepG2 cells. The flow-cytometry analysis was used to evaluate cell apoptosis, indicating that 74–95% of cells were apoptotic. OADP caused cell cycle arrest in the G0/G1 phase and the loss of the mitochondrial membrane potential (MMP). Western blot analysis was performed to assess the expression levels of key proteins associated with the underlying molecular mechanism. The results showed the clear upregulation of caspase-8, caspase-9, caspase-3, Bak, p21, and p53, accompanied by the downregula...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5cba898821c56dfb486e5e6185d06a43" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":84719293,"asset_id":77328034,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/84719293/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77328034"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77328034"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77328034; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77328034]").text(description); $(".js-view-count[data-work-id=77328034]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77328034; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77328034']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77328034, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5cba898821c56dfb486e5e6185d06a43" } } $('.js-work-strip[data-work-id=77328034]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77328034,"title":"A Diamine-PEGylated Oleanolic Acid Derivative Induced Efficient Apoptosis through a Death Receptor and Mitochondrial Apoptotic Pathway in HepG2 Human Hepatoma Cells","translated_title":"","metadata":{"abstract":"Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have shown that the diamine-(PEG)ylated oleanolic acid (OADP) has strong anti-tumor effects in HCCs. In this study, we evaluated the anti-tumor mechanisms of OADP in the HepG2 HCC cell line. The cytotoxicity results showed that HepG2 cell viability was markedly reduced, with a very low 50% of cell growth inhibitory concentration (IC50, 0.14 µg/mL). We then investigated the anti-tumor mechanisms of OADP in HepG2 cells. The flow-cytometry analysis was used to evaluate cell apoptosis, indicating that 74–95% of cells were apoptotic. OADP caused cell cycle arrest in the G0/G1 phase and the loss of the mitochondrial membrane potential (MMP). Western blot analysis was performed to assess the expression levels of key proteins associated with the underlying molecular mechanism. The results showed the clear upregulation of caspase-8, caspase-9, caspase-3, Bak, p21, and p53, accompanied by the downregula...","publisher":"MDPI AG","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Biomolecules"},"translated_abstract":"Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have shown that the diamine-(PEG)ylated oleanolic acid (OADP) has strong anti-tumor effects in HCCs. In this study, we evaluated the anti-tumor mechanisms of OADP in the HepG2 HCC cell line. The cytotoxicity results showed that HepG2 cell viability was markedly reduced, with a very low 50% of cell growth inhibitory concentration (IC50, 0.14 µg/mL). We then investigated the anti-tumor mechanisms of OADP in HepG2 cells. The flow-cytometry analysis was used to evaluate cell apoptosis, indicating that 74–95% of cells were apoptotic. OADP caused cell cycle arrest in the G0/G1 phase and the loss of the mitochondrial membrane potential (MMP). Western blot analysis was performed to assess the expression levels of key proteins associated with the underlying molecular mechanism. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136318"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/76136318/Influence_of_saccharides_and_sodium_chloride_on_growth_and_differentiation_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Influence of saccharides and sodium chloride on growth and differentiation of Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/83803961/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/76136318/Influence_of_saccharides_and_sodium_chloride_on_growth_and_differentiation_of_Trypanosoma_cruzi">Influence of saccharides and sodium chloride on growth and differentiation of Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>Cell Differentiation</span><span>, 1988</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="06354f29b6e4c5f8b93bcd0f2d6e556b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":83803961,"asset_id":76136318,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/83803961/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136318"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136318"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136318; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136310"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/76136310/Influence_of_electrolytes_and_non_electrolytes_on_growth_and_differentiation_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/76136310/Influence_of_electrolytes_and_non_electrolytes_on_growth_and_differentiation_of_Trypanosoma_cruzi">Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacycl...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136310"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136310"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136310; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=76136310]").text(description); $(".js-view-count[data-work-id=76136310]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 76136310; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='76136310']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 76136310, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=76136310]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":76136310,"title":"Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.","publication_date":{"day":null,"month":null,"year":1990,"errors":{}}},"translated_abstract":"The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.","internal_url":"https://www.academia.edu/76136310/Influence_of_electrolytes_and_non_electrolytes_on_growth_and_differentiation_of_Trypanosoma_cruzi","translated_internal_url":"","created_at":"2022-04-11T09:38:14.027-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Influence_of_electrolytes_and_non_electrolytes_on_growth_and_differentiation_of_Trypanosoma_cruzi","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":710903,"name":"Sorbitol","url":"https://www.academia.edu/Documents/in/Sorbitol"},{"id":897823,"name":"Elsevier","url":"https://www.academia.edu/Documents/in/Elsevier"},{"id":1223913,"name":"Sodium Chloride","url":"https://www.academia.edu/Documents/in/Sodium_Chloride"},{"id":3881526,"name":"In Vitro Techniques","url":"https://www.academia.edu/Documents/in/In_Vitro_Techniques"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136304"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/76136304/Differential_behaviour_of_glucose_6_phosphate_dehydrogenase_in_two_morphological_forms_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Differential behaviour of glucose 6-phosphate dehydrogenase in two morphological forms of Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/83804079/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/76136304/Differential_behaviour_of_glucose_6_phosphate_dehydrogenase_in_two_morphological_forms_of_Trypanosoma_cruzi">Differential behaviour of glucose 6-phosphate dehydrogenase in two morphological forms of Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>International Journal of Biochemistry</span><span>, 1987</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosom...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosoma cruzi, epimastigotes and metacyclics, are reported. 2. The kinetic behaviour and some of the kinetic parameters of the enzyme in both forms were studied. The enzymes showed a simple Michaelis-Menten kinetic. 3. The activity in epimastigote forms was alway higher than the metacyclic ones. At subsaturating concentrations of substrate was almost IO-fold higher, whereas at saturating concentrations was about 2-fold higher. 4. In epimastigote forms, the specific activity and K,,, values, at pH 7.5 and 37"C, was found to be 142 mUnits x mgg' of protein and 0.23 mM, respectively. 5. In the same conditions, the specific activity and K,,, values in metacyclic forms was 75 mUnits x mg-' of protein and I .06 mM, respectively. 6. A possible role in the carbohydrate metabolism of glucose 6-phosphate dehydrogenase in both forms of Trypanosoma cruzi is discussed.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4453ff4b51903437b896f835eee9a593" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":83804079,"asset_id":76136304,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/83804079/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136304"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136304"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136304; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=76136304]").text(description); $(".js-view-count[data-work-id=76136304]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 76136304; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='76136304']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 76136304, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4453ff4b51903437b896f835eee9a593" } } $('.js-work-strip[data-work-id=76136304]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":76136304,"title":"Differential behaviour of glucose 6-phosphate dehydrogenase in two morphological forms of Trypanosoma cruzi","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosoma cruzi, epimastigotes and metacyclics, are reported. 2. 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A possible role in the carbohydrate metabolism of glucose 6-phosphate dehydrogenase in both forms of Trypanosoma cruzi is discussed.","publication_date":{"day":null,"month":null,"year":1987,"errors":{}},"publication_name":"International Journal of Biochemistry","grobid_abstract_attachment_id":83804079},"translated_abstract":null,"internal_url":"https://www.academia.edu/76136304/Differential_behaviour_of_glucose_6_phosphate_dehydrogenase_in_two_morphological_forms_of_Trypanosoma_cruzi","translated_internal_url":"","created_at":"2022-04-11T09:38:12.138-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":83804079,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/83804079/thumbnails/1.jpg","file_name":"0020-711x_2887_2990310-720220411-1-a7z1gi.pdf","download_url":"https://www.academia.edu/attachments/83804079/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Differential_behaviour_of_glucose_6_phos.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/83804079/0020-711x_2887_2990310-720220411-1-a7z1gi-libre.pdf?1649696593=\u0026response-content-disposition=attachment%3B+filename%3DDifferential_behaviour_of_glucose_6_phos.pdf\u0026Expires=1736936359\u0026Signature=Mvg8E~E62qX2LTwG9vvuEEUBxY3TaZoZ1bagZaqbjVe4H58peHmZ5FlfQdVOO9vRXtK9Ho3Uni~I8gXYsB7YN74LvM5Odb~YJkGYC6zV4Y0tTIEcfHpyQKf-1NQUwiCm~3AiptCTuMMCGQcVIFTZP0-4-4MkXJFqBYFNNi32xasClVQqDkl6qSzv0UARHw7ShOOpA469AZ74dlb1zTZcJ97ZDbezxQPiEpZ3CAKV7r7aUujEZQ7xOaZuo-oVMwg9ZlSEeKKH5kWd2Yaau0hJapLT6lpl7P~IMh8RqdxyzjeQ18DZtQpX1VcxDkm9w5X6VO09t9SV4iuXxKJqWctygA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Differential_behaviour_of_glucose_6_phosphate_dehydrogenase_in_two_morphological_forms_of_Trypanosoma_cruzi","translated_slug":"","page_count":5,"language":"en","content_type":"Work","summary":"Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosoma cruzi, epimastigotes and metacyclics, are reported. 2. The kinetic behaviour and some of the kinetic parameters of the enzyme in both forms were studied. The enzymes showed a simple Michaelis-Menten kinetic. 3. The activity in epimastigote forms was alway higher than the metacyclic ones. At subsaturating concentrations of substrate was almost IO-fold higher, whereas at saturating concentrations was about 2-fold higher. 4. In epimastigote forms, the specific activity and K,,, values, at pH 7.5 and 37\"C, was found to be 142 mUnits x mgg' of protein and 0.23 mM, respectively. 5. In the same conditions, the specific activity and K,,, values in metacyclic forms was 75 mUnits x mg-' of protein and I .06 mM, respectively. 6. A possible role in the carbohydrate metabolism of glucose 6-phosphate dehydrogenase in both forms of Trypanosoma cruzi is discussed.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":83804079,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/83804079/thumbnails/1.jpg","file_name":"0020-711x_2887_2990310-720220411-1-a7z1gi.pdf","download_url":"https://www.academia.edu/attachments/83804079/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Differential_behaviour_of_glucose_6_phos.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/83804079/0020-711x_2887_2990310-720220411-1-a7z1gi-libre.pdf?1649696593=\u0026response-content-disposition=attachment%3B+filename%3DDifferential_behaviour_of_glucose_6_phos.pdf\u0026Expires=1736936359\u0026Signature=Mvg8E~E62qX2LTwG9vvuEEUBxY3TaZoZ1bagZaqbjVe4H58peHmZ5FlfQdVOO9vRXtK9Ho3Uni~I8gXYsB7YN74LvM5Odb~YJkGYC6zV4Y0tTIEcfHpyQKf-1NQUwiCm~3AiptCTuMMCGQcVIFTZP0-4-4MkXJFqBYFNNi32xasClVQqDkl6qSzv0UARHw7ShOOpA469AZ74dlb1zTZcJ97ZDbezxQPiEpZ3CAKV7r7aUujEZQ7xOaZuo-oVMwg9ZlSEeKKH5kWd2Yaau0hJapLT6lpl7P~IMh8RqdxyzjeQ18DZtQpX1VcxDkm9w5X6VO09t9SV4iuXxKJqWctygA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":51531,"name":"Pergamon","url":"https://www.academia.edu/Documents/in/Pergamon"},{"id":219724,"name":"Ammonia","url":"https://www.academia.edu/Documents/in/Ammonia"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136298"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/76136298/Influence_of_some_amino_acids_on_multiplication_and_metacyclogenesis_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Influence of some amino acids on multiplication and metacyclogenesis of Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/83803933/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/76136298/Influence_of_some_amino_acids_on_multiplication_and_metacyclogenesis_of_Trypanosoma_cruzi">Influence of some amino acids on multiplication and metacyclogenesis of Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>Chemosphere</span><span>, 1990</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A two-lane extension of a recently proposed cellular automaton model for traffic flow is discusse...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A two-lane extension of a recently proposed cellular automaton model for traffic flow is discussed. The analysis focuses on the reproduction of the lane usage inversion and the density dependence of the number of lane changes. It is shown that the single-lane dynamics can be extended to the two-lane case without changing the basic properties of the model which are known to be in good agreement with empirical single-vehicle data. Therefore it is possible to reproduce various empirically observed two-lane phenomena, like the synchronization of the lanes, without fine-tuning of the model parameters.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d4690a165d1be700cb1d06b587426d69" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":83803933,"asset_id":76136298,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/83803933/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136298"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136298"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136298; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=76136298]").text(description); $(".js-view-count[data-work-id=76136298]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 76136298; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='76136298']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 76136298, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d4690a165d1be700cb1d06b587426d69" } } $('.js-work-strip[data-work-id=76136298]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":76136298,"title":"Influence of some amino acids on multiplication and metacyclogenesis of Trypanosoma cruzi","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"A two-lane extension of a recently proposed cellular automaton model for traffic flow is discussed. 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Therefore it is possible to reproduce various empirically observed two-lane phenomena, like the synchronization of the lanes, without fine-tuning of the model parameters.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":83803933,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/83803933/thumbnails/1.jpg","file_name":"0203346.pdf","download_url":"https://www.academia.edu/attachments/83803933/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Influence_of_some_amino_acids_on_multipl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/83803933/0203346-libre.pdf?1649695886=\u0026response-content-disposition=attachment%3B+filename%3DInfluence_of_some_amino_acids_on_multipl.pdf\u0026Expires=1736936359\u0026Signature=g6PKXvpBLc5vgk03ksr3cj1HfsXK6xXYpWc0e-s3iGTCqTuIrTQHPbV1OezEjT~pcEPnovM3T0CxP1DvCev12b8tVP7d-ewQhSZIYQJD3GbWEVBxP3tJSMOOe8uDtQp2zLl79nZoyatL18trOBkxVMxanCR5SIlG5983lxbtByTVUxts2TEf6Tgg66STWLcOcml~CA7LxwTKH7AEtSIIkGCwxKxOHqPc7ib69Dp6BLNZhZLLTG5lIjGq0EpvK~Fq1qrtsXQujatoiqJhQCuGXCwvUuCy~Yy7KjB7OY0EC8ER2dnL5uht6mT2loJBSHSz6~HSYOeXq~og-6ARYAI9-w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":51531,"name":"Pergamon","url":"https://www.academia.edu/Documents/in/Pergamon"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":585573,"name":"Amino Acid Profile","url":"https://www.academia.edu/Documents/in/Amino_Acid_Profile"}],"urls":[]}, dispatcherData: dispatcherData }); 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The effects of osmotic pressure on crystal formation, as well as glucose consumption and ammonium excretion, were also evaluated. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136279"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/76136279/Is_there_thiaminase_activity_in_Rhodnius_prolixus_Hemiptera_Reduviidae_"><img alt="Research paper thumbnail of Is there thiaminase activity in Rhodnius prolixus (Hemiptera: Reduviidae)?" class="work-thumbnail" src="https://attachments.academia-assets.com/83803928/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/76136279/Is_there_thiaminase_activity_in_Rhodnius_prolixus_Hemiptera_Reduviidae_">Is there thiaminase activity in Rhodnius prolixus (Hemiptera: Reduviidae)?</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABS'rRAcr: Studies were performed in order to elucidate if thiamine changes are related to the in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABS'rRAcr: Studies were performed in order to elucidate if thiamine changes are related to the increase of the percentage of metacyclogenesis of Trypanosoma cruzi when cultivated in Grace's medium and an extract of Rhodnius prolixus is added. A lowering of thiamine levels in a modified version of Grace's medium was confirmed. Free thiamine decreased or disappeared from the medium when the extracts were present. It is clear from the results obtained that thiamine consumption was dependent on a saturation phenomenon. Attempts to identify any enzyme activity in the et(tract of R. prolixus, which might be responsiblc for reducing thiamine levels and might also cause a similar effect in the insect's gut, thus stimulating metacyclogenesis, proved however unsuccessful. KrY Wonos: Rhodnius prolixus' Chagas'disease vector, thiamine, thiaminase activity, metacyclogenesis of Tiypanosoma cruzi. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="1711690" id="papers"><div class="js-work-strip profile--work_container" data-work-id="94793714"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94793714/Efficient_In_Vitro_and_In_Vivo_Anti_Inflammatory_Activity_of_a_Diamine_PEGylated_Oleanolic_Acid_Derivative"><img alt="Research paper thumbnail of Efficient In Vitro and In Vivo Anti-Inflammatory Activity of a Diamine-PEGylated Oleanolic Acid Derivative" class="work-thumbnail" src="https://attachments.academia-assets.com/97153357/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94793714/Efficient_In_Vitro_and_In_Vivo_Anti_Inflammatory_Activity_of_a_Diamine_PEGylated_Oleanolic_Acid_Derivative">Efficient In Vitro and In Vivo Anti-Inflammatory Activity of a Diamine-PEGylated Oleanolic Acid Derivative</a></div><div class="wp-workCard_item"><span>International Journal of Molecular Sciences</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recent evidence has shown that inflammation can contribute to all tumorigenic states. We have inv...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recent evidence has shown that inflammation can contribute to all tumorigenic states. We have investigated the anti-inflammatory effects of a diamine-PEGylated derivative of oleanolic acid (OADP), in vitro and in vivo with inflammation models. In addition, we have determined the sub-cytotoxic concentrations for anti-inflammatory assays of OADP in RAW 264.7 cells. The inflammatory process began with incubation with lipopolysaccharide (LPS). Nitric oxide production levels were also determined, exceeding 75% inhibition of NO for a concentration of 1 µg/mL of OADP. Cell-cycle analysis showed a reversal of the arrest in the G0/G1 phase in LPS-stimulated RAW 264.7 cells. Furthermore, through Western blot analysis, we have determined the probable molecular mechanism activated by OADP; the inhibition of the expression of cytokines such as TNF-α, IL-1β, iNOS, and COX-2; and the blocking of p-IκBα production in LPS-stimulated RAW 264.7 cells. Finally, we have analyzed the anti-inflammatory ac...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="57432f0d6f73244dafd33bc8ebfc5340" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":97153357,"asset_id":94793714,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/97153357/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94793714"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94793714"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94793714; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94793714]").text(description); $(".js-view-count[data-work-id=94793714]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94793714; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94793714']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94793714, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "57432f0d6f73244dafd33bc8ebfc5340" } } $('.js-work-strip[data-work-id=94793714]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94793714,"title":"Efficient In Vitro and In Vivo Anti-Inflammatory Activity of a Diamine-PEGylated Oleanolic Acid Derivative","translated_title":"","metadata":{"abstract":"Recent evidence has shown that inflammation can contribute to all tumorigenic states. 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We have investigated the anti-inflammatory effects of a diamine-PEGylated derivative of oleanolic acid (OADP), in vitro and in vivo with inflammation models. In addition, we have determined the sub-cytotoxic concentrations for anti-inflammatory assays of OADP in RAW 264.7 cells. The inflammatory process began with incubation with lipopolysaccharide (LPS). Nitric oxide production levels were also determined, exceeding 75% inhibition of NO for a concentration of 1 µg/mL of OADP. Cell-cycle analysis showed a reversal of the arrest in the G0/G1 phase in LPS-stimulated RAW 264.7 cells. Furthermore, through Western blot analysis, we have determined the probable molecular mechanism activated by OADP; the inhibition of the expression of cytokines such as TNF-α, IL-1β, iNOS, and COX-2; and the blocking of p-IκBα production in LPS-stimulated RAW 264.7 cells. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94793712"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94793712/A_New_HPLC_MS_Method_for_Measuring_Maslinic_Acid_and_Oleanolic_Acid_in_HT29_and_HepG2_Human_Cancer_Cells"><img alt="Research paper thumbnail of A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/97153378/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94793712/A_New_HPLC_MS_Method_for_Measuring_Maslinic_Acid_and_Oleanolic_Acid_in_HT29_and_HepG2_Human_Cancer_Cells">A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells</a></div><div class="wp-workCard_item"><span>International Journal of Molecular Sciences</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have imp...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bdbbdaae87b4d652f515be08fbcccb39" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":97153378,"asset_id":94793712,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/97153378/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94793712"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94793712"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94793712; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94793712]").text(description); $(".js-view-count[data-work-id=94793712]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94793712; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94793712']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94793712, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bdbbdaae87b4d652f515be08fbcccb39" } } $('.js-work-strip[data-work-id=94793712]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94793712,"title":"A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells","translated_title":"","metadata":{"publisher":"MDPI AG","grobid_abstract":"Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. 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The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.","publication_date":{"day":null,"month":null,"year":2015,"errors":{}},"publication_name":"International Journal of Molecular Sciences","grobid_abstract_attachment_id":97153378},"translated_abstract":null,"internal_url":"https://www.academia.edu/94793712/A_New_HPLC_MS_Method_for_Measuring_Maslinic_Acid_and_Oleanolic_Acid_in_HT29_and_HepG2_Human_Cancer_Cells","translated_internal_url":"","created_at":"2023-01-11T10:48:34.535-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":97153378,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/97153378/thumbnails/1.jpg","file_name":"e83608b5f0f7ecec56be9cc9163958f07f10.pdf","download_url":"https://www.academia.edu/attachments/97153378/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"A_New_HPLC_MS_Method_for_Measuring_Masli.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/97153378/e83608b5f0f7ecec56be9cc9163958f07f10-libre.pdf?1673478383=\u0026response-content-disposition=attachment%3B+filename%3DA_New_HPLC_MS_Method_for_Measuring_Masli.pdf\u0026Expires=1736936358\u0026Signature=hG6aDwOZ19XLB4v~BW7hNHPrzQliRn98Bde73kF9vC12aQFhRQBYPTLTb4ocktznBBqiHU9mFWhI0bGiI-jUNfOsLbjrI92EEkh9RuV52AA5rGENOuz9vWJLVgaubB-5HrI654fJOXcP3ftfYhqe3S-gR9-c4mUlAdr3S7z6QsctRmObnQftUsI9kwB8NC~WIaqUC1Q-FrZaNpiKK1yHytWWS5jOBnKq5p6PaiRF7FDfLA8g8SPWqH23QNA3bs50iEfDlDytzc1jAy1GbJYiRdzxLswfE00~vCvxx-VFF5nAiNfzWccGrSsAgO4oNOa10jzZRO1-CvTWgXxKYADejg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_New_HPLC_MS_Method_for_Measuring_Maslinic_Acid_and_Oleanolic_Acid_in_HT29_and_HepG2_Human_Cancer_Cells","translated_slug":"","page_count":14,"language":"en","content_type":"Work","summary":"Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":97153378,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/97153378/thumbnails/1.jpg","file_name":"e83608b5f0f7ecec56be9cc9163958f07f10.pdf","download_url":"https://www.academia.edu/attachments/97153378/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"A_New_HPLC_MS_Method_for_Measuring_Masli.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/97153378/e83608b5f0f7ecec56be9cc9163958f07f10-libre.pdf?1673478383=\u0026response-content-disposition=attachment%3B+filename%3DA_New_HPLC_MS_Method_for_Measuring_Masli.pdf\u0026Expires=1736936358\u0026Signature=hG6aDwOZ19XLB4v~BW7hNHPrzQliRn98Bde73kF9vC12aQFhRQBYPTLTb4ocktznBBqiHU9mFWhI0bGiI-jUNfOsLbjrI92EEkh9RuV52AA5rGENOuz9vWJLVgaubB-5HrI654fJOXcP3ftfYhqe3S-gR9-c4mUlAdr3S7z6QsctRmObnQftUsI9kwB8NC~WIaqUC1Q-FrZaNpiKK1yHytWWS5jOBnKq5p6PaiRF7FDfLA8g8SPWqH23QNA3bs50iEfDlDytzc1jAy1GbJYiRdzxLswfE00~vCvxx-VFF5nAiNfzWccGrSsAgO4oNOa10jzZRO1-CvTWgXxKYADejg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":37782,"name":"Cell Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture"},{"id":102739,"name":"Ic","url":"https://www.academia.edu/Documents/in/Ic"},{"id":225787,"name":"High Performance Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Performance_Liquid_Chromatography"},{"id":276821,"name":"Molecular sciences","url":"https://www.academia.edu/Documents/in/Molecular_sciences"},{"id":506082,"name":"Cancer Cell","url":"https://www.academia.edu/Documents/in/Cancer_Cell"},{"id":1627795,"name":"Oleanolic acid","url":"https://www.academia.edu/Documents/in/Oleanolic_acid"},{"id":3589672,"name":"maslinic acid","url":"https://www.academia.edu/Documents/in/maslinic_acid"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94793691"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94793691/Diclofenac_N_Derivatives_as_Therapeutic_Agents_with_Anti_Inflammatory_and_Anti_Cancer_Effect"><img alt="Research paper thumbnail of Diclofenac N-Derivatives as Therapeutic Agents with Anti-Inflammatory and Anti-Cancer Effect" class="work-thumbnail" src="https://attachments.academia-assets.com/97153341/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94793691/Diclofenac_N_Derivatives_as_Therapeutic_Agents_with_Anti_Inflammatory_and_Anti_Cancer_Effect">Diclofenac N-Derivatives as Therapeutic Agents with Anti-Inflammatory and Anti-Cancer Effect</a></div><div class="wp-workCard_item"><span>International Journal of Molecular Sciences</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A series of diclofenac N-derivatives (2, 4, 6, 8c, 9c, 10a-c) were synthesized in order to test t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A series of diclofenac N-derivatives (2, 4, 6, 8c, 9c, 10a-c) were synthesized in order to test their anti-cancer and anti-inflammatory effects. The anticarcinogen activity has been assayed against three cancer cell lines: HT29, human colon cancer cells; Hep-G2, human hepatic cells; and B16-F10, murine melanoma cells. First, we determined the cytotoxicity of the different compounds, finding that the most effective compound was compound 8c against all cell lines and both compounds 4 and 6 in human Hep-G2 and HT29 cell lines. Compounds 4 and 8c were selected for the percentage of apoptosis determination, cell cycle distribution, and mitochondrial membrane potential measure because these products presented the lowest IC50 values in two of the three cancer cell lines assayed (B16-F10 and HepG2), and were two of the three products with lowest IC50 in HT29 cell line. Moreover, the percentages of apoptosis induction were determined for compounds 4 and 8c, showing that the highest values we...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4a02842e46ab44d20265db9d25b8b903" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":97153341,"asset_id":94793691,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/97153341/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94793691"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94793691"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94793691; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94793691]").text(description); $(".js-view-count[data-work-id=94793691]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94793691; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94793691']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 94793691, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4a02842e46ab44d20265db9d25b8b903" } } $('.js-work-strip[data-work-id=94793691]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94793691,"title":"Diclofenac N-Derivatives as Therapeutic Agents with Anti-Inflammatory and Anti-Cancer Effect","translated_title":"","metadata":{"abstract":"A series of diclofenac N-derivatives (2, 4, 6, 8c, 9c, 10a-c) were synthesized in order to test their anti-cancer and anti-inflammatory effects. The anticarcinogen activity has been assayed against three cancer cell lines: HT29, human colon cancer cells; Hep-G2, human hepatic cells; and B16-F10, murine melanoma cells. First, we determined the cytotoxicity of the different compounds, finding that the most effective compound was compound 8c against all cell lines and both compounds 4 and 6 in human Hep-G2 and HT29 cell lines. Compounds 4 and 8c were selected for the percentage of apoptosis determination, cell cycle distribution, and mitochondrial membrane potential measure because these products presented the lowest IC50 values in two of the three cancer cell lines assayed (B16-F10 and HepG2), and were two of the three products with lowest IC50 in HT29 cell line. 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Compounds 4 and 8c were selected for the percentage of apoptosis determination, cell cycle distribution, and mitochondrial membrane potential measure because these products presented the lowest IC50 values in two of the three cancer cell lines assayed (B16-F10 and HepG2), and were two of the three products with lowest IC50 in HT29 cell line. Moreover, the percentages of apoptosis induction were determined for compounds 4 and 8c, showing that the highest values we...","internal_url":"https://www.academia.edu/94793691/Diclofenac_N_Derivatives_as_Therapeutic_Agents_with_Anti_Inflammatory_and_Anti_Cancer_Effect","translated_internal_url":"","created_at":"2023-01-11T10:47:59.157-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":97153341,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/97153341/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/97153341/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Diclofenac_N_Derivatives_as_Therapeutic.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/97153341/pdf-libre.pdf?1673478374=\u0026response-content-disposition=attachment%3B+filename%3DDiclofenac_N_Derivatives_as_Therapeutic.pdf\u0026Expires=1736936358\u0026Signature=BUOO7roTZOQiqdqUfgb4b5BmFXuWTAujPkd5wHQM3U5Iebr6HnWzX-6z6SIGvNLK0cCo5ymg-eTQE68hRVqWhaGzlmR1OCG7NODS54n7~ZZuRBf6OJ6XcOwbQUOI0vYOZBSAkXAQMO~99eQBpbhVQiIkNsMgGayGloCsIjLI4EeFtZBElUndDBvVzRxSDE7gmScYUSN5dwZKdTzTW1EAu5gkXD0ykzq5v-8eoX-kS2mIJXq1ETANNMQ8EhX9PdecibQ0FfvVaDDmL4U-MPiLMwq7NTtSuB8W9t-FU2s90MtvXNaxnW3NOniQk3HHAn1v5n5Uyke4-9yIeMqcVpjCmQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Diclofenac_N_Derivatives_as_Therapeutic_Agents_with_Anti_Inflammatory_and_Anti_Cancer_Effect","translated_slug":"","page_count":23,"language":"en","content_type":"Work","summary":"A series of diclofenac N-derivatives (2, 4, 6, 8c, 9c, 10a-c) were synthesized in order to test their anti-cancer and anti-inflammatory effects. 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class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87786071/The_oleanolic_acid_derivative_3_O_succinyl_28_O_benzyl_oleanolate_induces_apoptosis_in_B16_F10_melanoma_cells_via_the_mitochondrial_apoptotic_pathway"><img alt="Research paper thumbnail of The oleanolic acid derivative, 3-O-succinyl-28-O-benzyl oleanolate, induces apoptosis in B16–F10 melanoma cells via the mitochondrial apoptotic pathway" class="work-thumbnail" src="https://attachments.academia-assets.com/91904495/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87786071/The_oleanolic_acid_derivative_3_O_succinyl_28_O_benzyl_oleanolate_induces_apoptosis_in_B16_F10_melanoma_cells_via_the_mitochondrial_apoptotic_pathway">The oleanolic acid derivative, 3-O-succinyl-28-O-benzyl oleanolate, induces apoptosis in B16–F10 melanoma cells via the mitochondrial apoptotic pathway</a></div><div class="wp-workCard_item"><span>RSC Advances</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antiproliferative and proapoptotic effects of 3-O-succinyl-28-O-benzyl oleanolate on B16–F10 skin...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antiproliferative and proapoptotic effects of 3-O-succinyl-28-O-benzyl oleanolate on B16–F10 skin-melanoma cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="79969724fcf70bd7a23c333bf535dfeb" class="wp-workCard--action" rel="nofollow" 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Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/91904549/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87786070/Maslinic_Acid_a_Natural_Triterpene_Induces_a_Death_Receptor_Mediated_Apoptotic_Mechanism_in_Caco_2_p53_Deficient_Colon_Adenocarcinoma_Cells">Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells</a></div><div class="wp-workCard_item"><span>PLOS ONE</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of oli...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dosedependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases-8 and-3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cadee59bee13df2feae9d1c8c9c3e19d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":91904549,"asset_id":87786070,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/91904549/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87786070"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87786070"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87786070; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87786070]").text(description); $(".js-view-count[data-work-id=87786070]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87786070; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87786070']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87786070, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cadee59bee13df2feae9d1c8c9c3e19d" } } $('.js-work-strip[data-work-id=87786070]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87786070,"title":"Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells","translated_title":"","metadata":{"publisher":"Public Library of Science (PLoS)","grobid_abstract":"Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dosedependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases-8 and-3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"PLOS ONE","grobid_abstract_attachment_id":91904549},"translated_abstract":null,"internal_url":"https://www.academia.edu/87786070/Maslinic_Acid_a_Natural_Triterpene_Induces_a_Death_Receptor_Mediated_Apoptotic_Mechanism_in_Caco_2_p53_Deficient_Colon_Adenocarcinoma_Cells","translated_internal_url":"","created_at":"2022-10-03T02:11:54.139-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":91904549,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91904549/thumbnails/1.jpg","file_name":"7c3b9af457401ff32cf5a8e80aaa905e2c6a.pdf","download_url":"https://www.academia.edu/attachments/91904549/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_a_Natural_Triterpene_Induc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91904549/7c3b9af457401ff32cf5a8e80aaa905e2c6a-libre.pdf?1664788745=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_a_Natural_Triterpene_Induc.pdf\u0026Expires=1736936358\u0026Signature=ECJX10-53IgSbGcVUnlrycBsouHazwsmvoklKgdNvxCCoSuqj10oaVH3iBgJs6NILERGlkCs3eY1DtVHHf5DY9kzJJ~ZfmR5GBETPSO96TrbG14Wul4nXc8a2dFP7pX6lqT789Dd4BQVwk-cE~pq~CgXKBQrohvQnKJ47o53qH7RGL8vKXtB381p1IDm0ZoHPJ1te8UAU9sEny0rglQDjt-EjJCetZjqk32Pn~gOxux8SaONhKmO5WyjltCQ5RRuJrGzyBFRjznwk2NUU5ml8rEtOglB286ummpM4Ft-XejcG5TyNk-7tm5xSUlziWcnvIphdVqip~5x6yDPfn8wEw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Maslinic_Acid_a_Natural_Triterpene_Induces_a_Death_Receptor_Mediated_Apoptotic_Mechanism_in_Caco_2_p53_Deficient_Colon_Adenocarcinoma_Cells","translated_slug":"","page_count":16,"language":"en","content_type":"Work","summary":"Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dosedependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases-8 and-3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":91904549,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91904549/thumbnails/1.jpg","file_name":"7c3b9af457401ff32cf5a8e80aaa905e2c6a.pdf","download_url":"https://www.academia.edu/attachments/91904549/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_a_Natural_Triterpene_Induc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91904549/7c3b9af457401ff32cf5a8e80aaa905e2c6a-libre.pdf?1664788745=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_a_Natural_Triterpene_Induc.pdf\u0026Expires=1736936358\u0026Signature=ECJX10-53IgSbGcVUnlrycBsouHazwsmvoklKgdNvxCCoSuqj10oaVH3iBgJs6NILERGlkCs3eY1DtVHHf5DY9kzJJ~ZfmR5GBETPSO96TrbG14Wul4nXc8a2dFP7pX6lqT789Dd4BQVwk-cE~pq~CgXKBQrohvQnKJ47o53qH7RGL8vKXtB381p1IDm0ZoHPJ1te8UAU9sEny0rglQDjt-EjJCetZjqk32Pn~gOxux8SaONhKmO5WyjltCQ5RRuJrGzyBFRjznwk2NUU5ml8rEtOglB286ummpM4Ft-XejcG5TyNk-7tm5xSUlziWcnvIphdVqip~5x6yDPfn8wEw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry"},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome"},{"id":15719,"name":"Mitochondria","url":"https://www.academia.edu/Documents/in/Mitochondria"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation"},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation"},{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one"},{"id":247477,"name":"Caspase","url":"https://www.academia.edu/Documents/in/Caspase"},{"id":314125,"name":"Caco-2 cells","url":"https://www.academia.edu/Documents/in/Caco-2_cells"},{"id":501806,"name":"Protein Expression","url":"https://www.academia.edu/Documents/in/Protein_Expression"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"},{"id":1692059,"name":"Minimum inhibitory concentration","url":"https://www.academia.edu/Documents/in/Minimum_inhibitory_concentration"},{"id":1745478,"name":"Adenocarcinoma","url":"https://www.academia.edu/Documents/in/Adenocarcinoma"},{"id":1758650,"name":"Triterpenes","url":"https://www.academia.edu/Documents/in/Triterpenes"},{"id":3793278,"name":"Colonic Neoplasms","url":"https://www.academia.edu/Documents/in/Colonic_Neoplasms"}],"urls":[{"id":24416062,"url":"http://dx.plos.org/10.1371/journal.pone.0146178"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="87786051"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87786051/Maslinic_Acid_Enriched_Diet_Decreases_Intestinal_Tumorigenesis_in_ApcMin_Mice_through_Transcriptomic_and_Metabolomic_Reprogramming"><img alt="Research paper thumbnail of Maslinic Acid-Enriched Diet Decreases Intestinal Tumorigenesis in ApcMin/+ Mice through Transcriptomic and Metabolomic Reprogramming" class="work-thumbnail" src="https://attachments.academia-assets.com/91904531/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87786051/Maslinic_Acid_Enriched_Diet_Decreases_Intestinal_Tumorigenesis_in_ApcMin_Mice_through_Transcriptomic_and_Metabolomic_Reprogramming">Maslinic Acid-Enriched Diet Decreases Intestinal Tumorigenesis in ApcMin/+ Mice through Transcriptomic and Metabolomic Reprogramming</a></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Chemoprevention is a pragmatic approach to reduce the risk of colorectal cancer, one of the leadi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Chemoprevention is a pragmatic approach to reduce the risk of colorectal cancer, one of the leading causes of cancerrelated death in western countries. In this regard, maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, is known to inhibit proliferation and induce apoptosis in colon cancer cell lines without affecting normal intestinal cells. The present study evaluated the chemopreventive efficacy and associated mechanisms of maslinic acid treatment on spontaneous intestinal tumorigenesis in Apc Min/+ mice. Twenty-two mice were randomized into 2 groups: control group and MA group, fed with a maslinic acid-supplemented diet for six weeks. MA treatment reduced total intestinal polyp formation by 45% (P,0.01). Putative molecular mechanisms associated with suppressing intestinal polyposis in Apc Min/+ mice were investigated by comparing microarray expression profiles of MA-treated and control mice and by analyzing the serum metabolic profile using NMR techniques. The different expression phenotype induced by MA suggested that it exerts its chemopreventive action mainly by inhibiting cell-survival signaling and inflammation. These changes eventually induce G1-phase cell cycle arrest and apoptosis. Moreover, the metabolic changes induced by MA treatment were associated with a protective profile against intestinal tumorigenesis. These results show the efficacy and underlying mechanisms of MA against intestinal tumor development in the Apc Min/+ mice model, suggesting its chemopreventive potential against colorectal cancer.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2efdda220922496498d0e670b57a5775" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":91904531,"asset_id":87786051,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/91904531/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87786051"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87786051"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87786051; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87786051]").text(description); $(".js-view-count[data-work-id=87786051]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87786051; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87786051']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87786051, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2efdda220922496498d0e670b57a5775" } } $('.js-work-strip[data-work-id=87786051]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87786051,"title":"Maslinic Acid-Enriched Diet Decreases Intestinal Tumorigenesis in ApcMin/+ Mice through Transcriptomic and Metabolomic Reprogramming","translated_title":"","metadata":{"publisher":"Public Library of Science (PLoS)","grobid_abstract":"Chemoprevention is a pragmatic approach to reduce the risk of colorectal cancer, one of the leading causes of cancerrelated death in western countries. In this regard, maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, is known to inhibit proliferation and induce apoptosis in colon cancer cell lines without affecting normal intestinal cells. The present study evaluated the chemopreventive efficacy and associated mechanisms of maslinic acid treatment on spontaneous intestinal tumorigenesis in Apc Min/+ mice. Twenty-two mice were randomized into 2 groups: control group and MA group, fed with a maslinic acid-supplemented diet for six weeks. MA treatment reduced total intestinal polyp formation by 45% (P,0.01). Putative molecular mechanisms associated with suppressing intestinal polyposis in Apc Min/+ mice were investigated by comparing microarray expression profiles of MA-treated and control mice and by analyzing the serum metabolic profile using NMR techniques. The different expression phenotype induced by MA suggested that it exerts its chemopreventive action mainly by inhibiting cell-survival signaling and inflammation. These changes eventually induce G1-phase cell cycle arrest and apoptosis. Moreover, the metabolic changes induced by MA treatment were associated with a protective profile against intestinal tumorigenesis. These results show the efficacy and underlying mechanisms of MA against intestinal tumor development in the Apc Min/+ mice model, suggesting its chemopreventive potential against colorectal cancer.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"PLoS ONE","grobid_abstract_attachment_id":91904531},"translated_abstract":null,"internal_url":"https://www.academia.edu/87786051/Maslinic_Acid_Enriched_Diet_Decreases_Intestinal_Tumorigenesis_in_ApcMin_Mice_through_Transcriptomic_and_Metabolomic_Reprogramming","translated_internal_url":"","created_at":"2022-10-03T02:11:26.825-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":91904531,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91904531/thumbnails/1.jpg","file_name":"75e5cc0cf301a9482d1a0f2eb075f10b90f1.pdf","download_url":"https://www.academia.edu/attachments/91904531/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_Enriched_Diet_Decreases_In.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91904531/75e5cc0cf301a9482d1a0f2eb075f10b90f1-libre.pdf?1664788748=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_Enriched_Diet_Decreases_In.pdf\u0026Expires=1736936358\u0026Signature=JPR3HAhmx07eMUdqd~VlYZath~YLFseA5QNCGwNhFMMyqbamgQyvdCDzK5SZk4p~qohTKPdOGcjC1FlYjcL7XKP0Ld4QOeofYElzpCL~4SoJY3sgCoFkD6resMFCytH9ACgiLGxLQJ~86ZuXOqyc2cdiNX4VLC8GhOP6-h-opnePj0XzIZq9cwYaA4ykPgbLFsvc26A56u7MqSXyxzMyyrHnwkDbZkmsyPMnaIX43EX7ip5XZzkjrLeWbBYC9Ha6Bsr1dNMh6CkGqG3n4P5UREXaRe8J2sqhUY93OgyzqmuFnYn9s9zCUYv04lntPbO9jF9SkWtV4UdOQkhzyPXn0g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Maslinic_Acid_Enriched_Diet_Decreases_Intestinal_Tumorigenesis_in_ApcMin_Mice_through_Transcriptomic_and_Metabolomic_Reprogramming","translated_slug":"","page_count":11,"language":"en","content_type":"Work","summary":"Chemoprevention is a pragmatic approach to reduce the risk of colorectal cancer, one of the leading causes of cancerrelated death in western countries. In this regard, maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, is known to inhibit proliferation and induce apoptosis in colon cancer cell lines without affecting normal intestinal cells. The present study evaluated the chemopreventive efficacy and associated mechanisms of maslinic acid treatment on spontaneous intestinal tumorigenesis in Apc Min/+ mice. Twenty-two mice were randomized into 2 groups: control group and MA group, fed with a maslinic acid-supplemented diet for six weeks. MA treatment reduced total intestinal polyp formation by 45% (P,0.01). Putative molecular mechanisms associated with suppressing intestinal polyposis in Apc Min/+ mice were investigated by comparing microarray expression profiles of MA-treated and control mice and by analyzing the serum metabolic profile using NMR techniques. The different expression phenotype induced by MA suggested that it exerts its chemopreventive action mainly by inhibiting cell-survival signaling and inflammation. These changes eventually induce G1-phase cell cycle arrest and apoptosis. Moreover, the metabolic changes induced by MA treatment were associated with a protective profile against intestinal tumorigenesis. These results show the efficacy and underlying mechanisms of MA against intestinal tumor development in the Apc Min/+ mice model, suggesting its chemopreventive potential against colorectal cancer.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":91904531,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91904531/thumbnails/1.jpg","file_name":"75e5cc0cf301a9482d1a0f2eb075f10b90f1.pdf","download_url":"https://www.academia.edu/attachments/91904531/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Maslinic_Acid_Enriched_Diet_Decreases_In.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91904531/75e5cc0cf301a9482d1a0f2eb075f10b90f1-libre.pdf?1664788748=\u0026response-content-disposition=attachment%3B+filename%3DMaslinic_Acid_Enriched_Diet_Decreases_In.pdf\u0026Expires=1736936358\u0026Signature=JPR3HAhmx07eMUdqd~VlYZath~YLFseA5QNCGwNhFMMyqbamgQyvdCDzK5SZk4p~qohTKPdOGcjC1FlYjcL7XKP0Ld4QOeofYElzpCL~4SoJY3sgCoFkD6resMFCytH9ACgiLGxLQJ~86ZuXOqyc2cdiNX4VLC8GhOP6-h-opnePj0XzIZq9cwYaA4ykPgbLFsvc26A56u7MqSXyxzMyyrHnwkDbZkmsyPMnaIX43EX7ip5XZzkjrLeWbBYC9Ha6Bsr1dNMh6CkGqG3n4P5UREXaRe8J2sqhUY93OgyzqmuFnYn9s9zCUYv04lntPbO9jF9SkWtV4UdOQkhzyPXn0g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3770,"name":"Metabolism","url":"https://www.academia.edu/Documents/in/Metabolism"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":37292,"name":"Colorectal cancer","url":"https://www.academia.edu/Documents/in/Colorectal_cancer"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":136837,"name":"Tumors","url":"https://www.academia.edu/Documents/in/Tumors"},{"id":152562,"name":"Dietary Supplements","url":"https://www.academia.edu/Documents/in/Dietary_Supplements"},{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one"},{"id":375301,"name":"Microarray Analysis","url":"https://www.academia.edu/Documents/in/Microarray_Analysis"},{"id":1198158,"name":"Metabolisme","url":"https://www.academia.edu/Documents/in/Metabolisme"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"},{"id":1758650,"name":"Triterpenes","url":"https://www.academia.edu/Documents/in/Triterpenes"},{"id":1810445,"name":"Gene expression profiling","url":"https://www.academia.edu/Documents/in/Gene_expression_profiling"},{"id":2940273,"name":"real time polymerase chain reaction","url":"https://www.academia.edu/Documents/in/real_time_polymerase_chain_reaction"},{"id":3067128,"name":"reverse transcriptase polymerase chain reaction","url":"https://www.academia.edu/Documents/in/reverse_transcriptase_polymerase_chain_reaction"}],"urls":[{"id":24416053,"url":"http://dx.plos.org/10.1371/journal.pone.0059392"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="86649934"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/86649934/Unveiling_the_Differential_Anti_Oxidant_Activity_of_Maslinic_Acid_in_Murine_Melanoma_Cells_and_in_Rat_Embryonic_Healthy_Cells_Following_Treatment_With_Hydrogen_Peroxide"><img alt="Research paper thumbnail of Unveiling the Differential Anti-Oxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment With Hydrogen Peroxide" class="work-thumbnail" src="https://attachments.academia-assets.com/91052747/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/86649934/Unveiling_the_Differential_Anti_Oxidant_Activity_of_Maslinic_Acid_in_Murine_Melanoma_Cells_and_in_Rat_Embryonic_Healthy_Cells_Following_Treatment_With_Hydrogen_Peroxide">Unveiling the Differential Anti-Oxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment With Hydrogen Peroxide</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions hav...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM) whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM of H2O2 were added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, being higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in anti-oxidant enzymes activities depending on cell line. In A10 cells, all enzymes were up-regulated, but in B16F10 cells only superoxide dismutase, glutathione S-transferase ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1650216311fd560848108e317cff3fbf" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":91052747,"asset_id":86649934,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/91052747/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="86649934"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="86649934"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 86649934; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=86649934]").text(description); $(".js-view-count[data-work-id=86649934]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 86649934; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='86649934']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 86649934, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1650216311fd560848108e317cff3fbf" } } $('.js-work-strip[data-work-id=86649934]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":86649934,"title":"Unveiling the Differential Anti-Oxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment With Hydrogen Peroxide","translated_title":"","metadata":{"abstract":"Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM) whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM of H2O2 were added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, being higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in anti-oxidant enzymes activities depending on cell line. In A10 cells, all enzymes were up-regulated, but in B16F10 cells only superoxide dismutase, glutathione S-transferase ...","publisher":"MDPI AG","publication_date":{"day":null,"month":null,"year":2020,"errors":{}}},"translated_abstract":"Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM) whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM of H2O2 were added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, being higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in anti-oxidant enzymes activities depending on cell line. In A10 cells, all enzymes were up-regulated, but in B16F10 cells only superoxide dismutase, glutathione S-transferase ...","internal_url":"https://www.academia.edu/86649934/Unveiling_the_Differential_Anti_Oxidant_Activity_of_Maslinic_Acid_in_Murine_Melanoma_Cells_and_in_Rat_Embryonic_Healthy_Cells_Following_Treatment_With_Hydrogen_Peroxide","translated_internal_url":"","created_at":"2022-09-14T10:42:04.040-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":91052747,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91052747/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/91052747/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Unveiling_the_Differential_Anti_Oxidant.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91052747/pdf-libre.pdf?1663177490=\u0026response-content-disposition=attachment%3B+filename%3DUnveiling_the_Differential_Anti_Oxidant.pdf\u0026Expires=1736936359\u0026Signature=TxDa04QaCQL9WvXFriMfACnWEjvxmVou6TaktEJgh66Q9ZeKPQIFhnQwyDVbrYGsFM5AiPxBV6um5F5qLNf-x0sBD3Vo4-5cdO9Q6xCcSanXydCXxB4rghBJjfp-KmtnbhmADuDUvNpjkXq7sxJba8-Onoizo-bRd3QN~0VmKPBjBd-cAeM6sv3C4ENNCDgJwfNU29N7IH05OJj1vHl2~2RH8nFHg3MLgcqzqoX3X9kb4v~tcORLzdgc0gUcfVyaAaOswwelgMjJVgmXRvWYvBAz~Q9rWrvx2RRZG2Oq2kIslcEidv5TWhxNgUyzkvfbUTRPm5x-rLCAQqLp5sEHgg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Unveiling_the_Differential_Anti_Oxidant_Activity_of_Maslinic_Acid_in_Murine_Melanoma_Cells_and_in_Rat_Embryonic_Healthy_Cells_Following_Treatment_With_Hydrogen_Peroxide","translated_slug":"","page_count":14,"language":"en","content_type":"Work","summary":"Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM) whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM of H2O2 were added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, being higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in anti-oxidant enzymes activities depending on cell line. In A10 cells, all enzymes were up-regulated, but in B16F10 cells only superoxide dismutase, glutathione S-transferase ...","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":91052747,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/91052747/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/91052747/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Unveiling_the_Differential_Anti_Oxidant.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/91052747/pdf-libre.pdf?1663177490=\u0026response-content-disposition=attachment%3B+filename%3DUnveiling_the_Differential_Anti_Oxidant.pdf\u0026Expires=1736936359\u0026Signature=TxDa04QaCQL9WvXFriMfACnWEjvxmVou6TaktEJgh66Q9ZeKPQIFhnQwyDVbrYGsFM5AiPxBV6um5F5qLNf-x0sBD3Vo4-5cdO9Q6xCcSanXydCXxB4rghBJjfp-KmtnbhmADuDUvNpjkXq7sxJba8-Onoizo-bRd3QN~0VmKPBjBd-cAeM6sv3C4ENNCDgJwfNU29N7IH05OJj1vHl2~2RH8nFHg3MLgcqzqoX3X9kb4v~tcORLzdgc0gUcfVyaAaOswwelgMjJVgmXRvWYvBAz~Q9rWrvx2RRZG2Oq2kIslcEidv5TWhxNgUyzkvfbUTRPm5x-rLCAQqLp5sEHgg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":531,"name":"Organic Chemistry","url":"https://www.academia.edu/Documents/in/Organic_Chemistry"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":290433,"name":"Antioxidant Activity","url":"https://www.academia.edu/Documents/in/Antioxidant_Activity"},{"id":328449,"name":"Molecules","url":"https://www.academia.edu/Documents/in/Molecules"},{"id":842299,"name":"Antioxidant enzymes","url":"https://www.academia.edu/Documents/in/Antioxidant_enzymes"},{"id":1485949,"name":"Preprints","url":"https://www.academia.edu/Documents/in/Preprints"},{"id":2284202,"name":"Anti-proliferative activity","url":"https://www.academia.edu/Documents/in/Anti-proliferative_activity"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="82718670"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/82718670/Maslinic_Acid_a_Triterpene_from_Olive_Affects_the_Antioxidant_and_Mitochondrial_Status_of_B16F10_Melanoma_Cells_Grown_under_Stressful_Conditions"><img alt="Research paper thumbnail of Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions" class="work-thumbnail" src="https://attachments.academia-assets.com/88333635/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/82718670/Maslinic_Acid_a_Triterpene_from_Olive_Affects_the_Antioxidant_and_Mitochondrial_Status_of_B16F10_Melanoma_Cells_Grown_under_Stressful_Conditions">Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions</a></div><div class="wp-workCard_item"><span>Evidence-Based Complementary and Alternative Medicine</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene....</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50values of MA. The DHR 123 data sh...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a2cc34971c865d9b932ddd0c9a669476" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88333635,"asset_id":82718670,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88333635/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="82718670"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="82718670"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 82718670; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=82718670]").text(description); $(".js-view-count[data-work-id=82718670]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 82718670; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='82718670']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 82718670, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a2cc34971c865d9b932ddd0c9a669476" } } $('.js-work-strip[data-work-id=82718670]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":82718670,"title":"Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions","translated_title":"","metadata":{"abstract":"Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. 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The modifications induced by pH shifts, rise growth ( 1.3-fold in Grace&amp;#39;s medium and 1.4-fold in MEM medium) and metacyclic forms percentage (3.9- and 5.9-fold, respectively). Possible influence of chemical and physico-chemical factors in these increases is proposed and discussed.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77328102"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77328102"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77328102; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77328102]").text(description); $(".js-view-count[data-work-id=77328102]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77328102; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77328102']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77328102, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77328102]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77328102,"title":"Influencia de las alteraciones de pH en los medios de cultivo para la obtención de formas metacíclicas de Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"The influence of several modifications of Grace and MEM (minimal essential medium) media on growth of Trypanosoma cruzi epimastigote forms and differentiation to metacyclic infective forms is studied. 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Possible influence of chemical and physico-chemical factors in these increases is proposed and discussed.","internal_url":"https://www.academia.edu/77328102/Influencia_de_las_alteraciones_de_pH_en_los_medios_de_cultivo_para_la_obtenci%C3%B3n_de_formas_metac%C3%ADclicas_de_Trypanosoma_cruzi","translated_internal_url":"","created_at":"2022-04-23T00:26:34.388-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Influencia_de_las_alteraciones_de_pH_en_los_medios_de_cultivo_para_la_obtención_de_formas_metacíclicas_de_Trypanosoma_cruzi","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The influence of several modifications of Grace and MEM (minimal essential medium) media on growth of Trypanosoma cruzi epimastigote forms and differentiation to metacyclic infective forms is studied. The modifications induced by pH shifts, rise growth ( 1.3-fold in Grace\u0026amp;#39;s medium and 1.4-fold in MEM medium) and metacyclic forms percentage (3.9- and 5.9-fold, respectively). Possible influence of chemical and physico-chemical factors in these increases is proposed and discussed.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":2477473,"name":"Metacyclogenesis","url":"https://www.academia.edu/Documents/in/Metacyclogenesis"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77328034"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77328034/A_Diamine_PEGylated_Oleanolic_Acid_Derivative_Induced_Efficient_Apoptosis_through_a_Death_Receptor_and_Mitochondrial_Apoptotic_Pathway_in_HepG2_Human_Hepatoma_Cells"><img alt="Research paper thumbnail of A Diamine-PEGylated Oleanolic Acid Derivative Induced Efficient Apoptosis through a Death Receptor and Mitochondrial Apoptotic Pathway in HepG2 Human Hepatoma Cells" class="work-thumbnail" src="https://attachments.academia-assets.com/84719293/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77328034/A_Diamine_PEGylated_Oleanolic_Acid_Derivative_Induced_Efficient_Apoptosis_through_a_Death_Receptor_and_Mitochondrial_Apoptotic_Pathway_in_HepG2_Human_Hepatoma_Cells">A Diamine-PEGylated Oleanolic Acid Derivative Induced Efficient Apoptosis through a Death Receptor and Mitochondrial Apoptotic Pathway in HepG2 Human Hepatoma Cells</a></div><div class="wp-workCard_item"><span>Biomolecules</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have s...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have shown that the diamine-(PEG)ylated oleanolic acid (OADP) has strong anti-tumor effects in HCCs. In this study, we evaluated the anti-tumor mechanisms of OADP in the HepG2 HCC cell line. The cytotoxicity results showed that HepG2 cell viability was markedly reduced, with a very low 50% of cell growth inhibitory concentration (IC50, 0.14 µg/mL). We then investigated the anti-tumor mechanisms of OADP in HepG2 cells. The flow-cytometry analysis was used to evaluate cell apoptosis, indicating that 74–95% of cells were apoptotic. OADP caused cell cycle arrest in the G0/G1 phase and the loss of the mitochondrial membrane potential (MMP). Western blot analysis was performed to assess the expression levels of key proteins associated with the underlying molecular mechanism. The results showed the clear upregulation of caspase-8, caspase-9, caspase-3, Bak, p21, and p53, accompanied by the downregula...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5cba898821c56dfb486e5e6185d06a43" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":84719293,"asset_id":77328034,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/84719293/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77328034"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77328034"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77328034; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77328034]").text(description); $(".js-view-count[data-work-id=77328034]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77328034; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77328034']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77328034, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5cba898821c56dfb486e5e6185d06a43" } } $('.js-work-strip[data-work-id=77328034]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77328034,"title":"A Diamine-PEGylated Oleanolic Acid Derivative Induced Efficient Apoptosis through a Death Receptor and Mitochondrial Apoptotic Pathway in HepG2 Human Hepatoma Cells","translated_title":"","metadata":{"abstract":"Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have shown that the diamine-(PEG)ylated oleanolic acid (OADP) has strong anti-tumor effects in HCCs. In this study, we evaluated the anti-tumor mechanisms of OADP in the HepG2 HCC cell line. The cytotoxicity results showed that HepG2 cell viability was markedly reduced, with a very low 50% of cell growth inhibitory concentration (IC50, 0.14 µg/mL). We then investigated the anti-tumor mechanisms of OADP in HepG2 cells. The flow-cytometry analysis was used to evaluate cell apoptosis, indicating that 74–95% of cells were apoptotic. OADP caused cell cycle arrest in the G0/G1 phase and the loss of the mitochondrial membrane potential (MMP). Western blot analysis was performed to assess the expression levels of key proteins associated with the underlying molecular mechanism. The results showed the clear upregulation of caspase-8, caspase-9, caspase-3, Bak, p21, and p53, accompanied by the downregula...","publisher":"MDPI AG","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Biomolecules"},"translated_abstract":"Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have shown that the diamine-(PEG)ylated oleanolic acid (OADP) has strong anti-tumor effects in HCCs. In this study, we evaluated the anti-tumor mechanisms of OADP in the HepG2 HCC cell line. The cytotoxicity results showed that HepG2 cell viability was markedly reduced, with a very low 50% of cell growth inhibitory concentration (IC50, 0.14 µg/mL). We then investigated the anti-tumor mechanisms of OADP in HepG2 cells. The flow-cytometry analysis was used to evaluate cell apoptosis, indicating that 74–95% of cells were apoptotic. OADP caused cell cycle arrest in the G0/G1 phase and the loss of the mitochondrial membrane potential (MMP). Western blot analysis was performed to assess the expression levels of key proteins associated with the underlying molecular mechanism. The results showed the clear upregulation of caspase-8, caspase-9, caspase-3, Bak, p21, and p53, accompanied by the downregula...","internal_url":"https://www.academia.edu/77328034/A_Diamine_PEGylated_Oleanolic_Acid_Derivative_Induced_Efficient_Apoptosis_through_a_Death_Receptor_and_Mitochondrial_Apoptotic_Pathway_in_HepG2_Human_Hepatoma_Cells","translated_internal_url":"","created_at":"2022-04-23T00:26:19.451-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":84719293,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/84719293/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/84719293/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"A_Diamine_PEGylated_Oleanolic_Acid_Deriv.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/84719293/pdf-libre.pdf?1650699439=\u0026response-content-disposition=attachment%3B+filename%3DA_Diamine_PEGylated_Oleanolic_Acid_Deriv.pdf\u0026Expires=1736936359\u0026Signature=f5lKYnJGYqhSa8CBz0X5WBfYE~TaHQxMhvS6b6hHYEeJ5mC9VgDTB~PiqrTnDAgUvfss8QhQvGzPSfXCdV9zj9L27yjjzZcie6MG39ttRjINQcnaeVu4nDLQ9dnXku34TwCO-qeqomAUWmChuhnjgNpX5HAH1-cjUOO5UYAPCzhYJqh~-BsyDJldKWvGthVy33ni~V~RoGwFPcGrDPHv3Hzf8puzTdtoIrgTDdA-yzrO32FgmimN4-OoNh7NfbGC68gJy9wx9UXYbF1p2UEX~O1oy0Vc50ufbZ6yGtIy3NOPtnTIovmnwrl3W~wJlDP4etLGDLmctD7nou543n0b1w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_Diamine_PEGylated_Oleanolic_Acid_Derivative_Induced_Efficient_Apoptosis_through_a_Death_Receptor_and_Mitochondrial_Apoptotic_Pathway_in_HepG2_Human_Hepatoma_Cells","translated_slug":"","page_count":22,"language":"en","content_type":"Work","summary":"Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have shown that the diamine-(PEG)ylated oleanolic acid (OADP) has strong anti-tumor effects in HCCs. In this study, we evaluated the anti-tumor mechanisms of OADP in the HepG2 HCC cell line. The cytotoxicity results showed that HepG2 cell viability was markedly reduced, with a very low 50% of cell growth inhibitory concentration (IC50, 0.14 µg/mL). We then investigated the anti-tumor mechanisms of OADP in HepG2 cells. The flow-cytometry analysis was used to evaluate cell apoptosis, indicating that 74–95% of cells were apoptotic. OADP caused cell cycle arrest in the G0/G1 phase and the loss of the mitochondrial membrane potential (MMP). Western blot analysis was performed to assess the expression levels of key proteins associated with the underlying molecular mechanism. The results showed the clear upregulation of caspase-8, caspase-9, caspase-3, Bak, p21, and p53, accompanied by the downregula...","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":84719293,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/84719293/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/84719293/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"A_Diamine_PEGylated_Oleanolic_Acid_Deriv.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/84719293/pdf-libre.pdf?1650699439=\u0026response-content-disposition=attachment%3B+filename%3DA_Diamine_PEGylated_Oleanolic_Acid_Deriv.pdf\u0026Expires=1736936359\u0026Signature=f5lKYnJGYqhSa8CBz0X5WBfYE~TaHQxMhvS6b6hHYEeJ5mC9VgDTB~PiqrTnDAgUvfss8QhQvGzPSfXCdV9zj9L27yjjzZcie6MG39ttRjINQcnaeVu4nDLQ9dnXku34TwCO-qeqomAUWmChuhnjgNpX5HAH1-cjUOO5UYAPCzhYJqh~-BsyDJldKWvGthVy33ni~V~RoGwFPcGrDPHv3Hzf8puzTdtoIrgTDdA-yzrO32FgmimN4-OoNh7NfbGC68gJy9wx9UXYbF1p2UEX~O1oy0Vc50ufbZ6yGtIy3NOPtnTIovmnwrl3W~wJlDP4etLGDLmctD7nou543n0b1w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":84719292,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/84719292/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/84719292/download_file","bulk_download_file_name":"A_Diamine_PEGylated_Oleanolic_Acid_Deriv.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/84719292/pdf-libre.pdf?1650699439=\u0026response-content-disposition=attachment%3B+filename%3DA_Diamine_PEGylated_Oleanolic_Acid_Deriv.pdf\u0026Expires=1736936359\u0026Signature=fEiO2rpWoqWgbZeWyeTiOTKTxIU3erNfsIr1LB6NxdJiytXrLmy34GMxDLPl-FkflbYrfQPQo2Dae7O~Yi9XPOYlDOKArjqPYXprMlYWyBcaMOdaKBS7s8JHD419VtRGFVGrsUihQ24Q44Wq8q0D21E19zW-JlhEg81zBTtBTaZyGEtuI37kVbCkQMZf2ymMen8KXYodPwZXNq77ylLxlR1dgo0Bq5GgyDnYO5T2C3hucPn9q3Q43nFJIt8k1D67C55DLpL34uAZ3qKoSfAXIFHaPDyVoUwLPDy9PMZ5yMdf9KgHta7x8v2qoN69qLiqKuFWumPsA7V4Co~LSQwrGQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":531,"name":"Organic Chemistry","url":"https://www.academia.edu/Documents/in/Organic_Chemistry"},{"id":13828,"name":"Cancer Cell Biology","url":"https://www.academia.edu/Documents/in/Cancer_Cell_Biology"},{"id":14195,"name":"Cancer Biology","url":"https://www.academia.edu/Documents/in/Cancer_Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":46992,"name":"Molecular Biology Cancer","url":"https://www.academia.edu/Documents/in/Molecular_Biology_Cancer"},{"id":78754,"name":"Cancer Therapy","url":"https://www.academia.edu/Documents/in/Cancer_Therapy"},{"id":402990,"name":"Biomolecules","url":"https://www.academia.edu/Documents/in/Biomolecules"}],"urls":[{"id":19806124,"url":"https://www.mdpi.com/2218-273X/10/10/1375/pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136318"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/76136318/Influence_of_saccharides_and_sodium_chloride_on_growth_and_differentiation_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Influence of saccharides and sodium chloride on growth and differentiation of Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/83803961/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/76136318/Influence_of_saccharides_and_sodium_chloride_on_growth_and_differentiation_of_Trypanosoma_cruzi">Influence of saccharides and sodium chloride on growth and differentiation of Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>Cell Differentiation</span><span>, 1988</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="06354f29b6e4c5f8b93bcd0f2d6e556b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":83803961,"asset_id":76136318,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/83803961/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136318"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136318"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136318; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136310"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/76136310/Influence_of_electrolytes_and_non_electrolytes_on_growth_and_differentiation_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/76136310/Influence_of_electrolytes_and_non_electrolytes_on_growth_and_differentiation_of_Trypanosoma_cruzi">Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacycl...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136310"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136310"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136310; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=76136310]").text(description); $(".js-view-count[data-work-id=76136310]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 76136310; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='76136310']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 76136310, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=76136310]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":76136310,"title":"Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.","publication_date":{"day":null,"month":null,"year":1990,"errors":{}}},"translated_abstract":"The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.","internal_url":"https://www.academia.edu/76136310/Influence_of_electrolytes_and_non_electrolytes_on_growth_and_differentiation_of_Trypanosoma_cruzi","translated_internal_url":"","created_at":"2022-04-11T09:38:14.027-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Influence_of_electrolytes_and_non_electrolytes_on_growth_and_differentiation_of_Trypanosoma_cruzi","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The influence of electrolytes and non-electrolytes, especially NaCl and sorbitol, on the metacyclogenesis and growth of Trypanosoma cruzi has been studied. The addition of 50 or 100 mEq/l NaCl to the culture media significantly increased the development of metacyclic forms. Other electrolytes and non-electrolytes had no effect on epimastigote-metacyclic differentiation. The growth rate was never modified to any extent. The influence of sodium concentration, osmotic pressure, among other factors, are discussed. Electrophoresis showed proteins bands which could be related either to the adaptation of T. cruzi to the new culture media or to the initiation of differentiation processes.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":710903,"name":"Sorbitol","url":"https://www.academia.edu/Documents/in/Sorbitol"},{"id":897823,"name":"Elsevier","url":"https://www.academia.edu/Documents/in/Elsevier"},{"id":1223913,"name":"Sodium Chloride","url":"https://www.academia.edu/Documents/in/Sodium_Chloride"},{"id":3881526,"name":"In Vitro Techniques","url":"https://www.academia.edu/Documents/in/In_Vitro_Techniques"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136304"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/76136304/Differential_behaviour_of_glucose_6_phosphate_dehydrogenase_in_two_morphological_forms_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Differential behaviour of glucose 6-phosphate dehydrogenase in two morphological forms of Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/83804079/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/76136304/Differential_behaviour_of_glucose_6_phosphate_dehydrogenase_in_two_morphological_forms_of_Trypanosoma_cruzi">Differential behaviour of glucose 6-phosphate dehydrogenase in two morphological forms of Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>International Journal of Biochemistry</span><span>, 1987</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosom...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosoma cruzi, epimastigotes and metacyclics, are reported. 2. The kinetic behaviour and some of the kinetic parameters of the enzyme in both forms were studied. The enzymes showed a simple Michaelis-Menten kinetic. 3. The activity in epimastigote forms was alway higher than the metacyclic ones. At subsaturating concentrations of substrate was almost IO-fold higher, whereas at saturating concentrations was about 2-fold higher. 4. In epimastigote forms, the specific activity and K,,, values, at pH 7.5 and 37"C, was found to be 142 mUnits x mgg' of protein and 0.23 mM, respectively. 5. In the same conditions, the specific activity and K,,, values in metacyclic forms was 75 mUnits x mg-' of protein and I .06 mM, respectively. 6. A possible role in the carbohydrate metabolism of glucose 6-phosphate dehydrogenase in both forms of Trypanosoma cruzi is discussed.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4453ff4b51903437b896f835eee9a593" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":83804079,"asset_id":76136304,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/83804079/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136304"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136304"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136304; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=76136304]").text(description); $(".js-view-count[data-work-id=76136304]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 76136304; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='76136304']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 76136304, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4453ff4b51903437b896f835eee9a593" } } $('.js-work-strip[data-work-id=76136304]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":76136304,"title":"Differential behaviour of glucose 6-phosphate dehydrogenase in two morphological forms of Trypanosoma cruzi","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosoma cruzi, epimastigotes and metacyclics, are reported. 2. 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A possible role in the carbohydrate metabolism of glucose 6-phosphate dehydrogenase in both forms of Trypanosoma cruzi is discussed.","publication_date":{"day":null,"month":null,"year":1987,"errors":{}},"publication_name":"International Journal of Biochemistry","grobid_abstract_attachment_id":83804079},"translated_abstract":null,"internal_url":"https://www.academia.edu/76136304/Differential_behaviour_of_glucose_6_phosphate_dehydrogenase_in_two_morphological_forms_of_Trypanosoma_cruzi","translated_internal_url":"","created_at":"2022-04-11T09:38:12.138-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":83804079,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/83804079/thumbnails/1.jpg","file_name":"0020-711x_2887_2990310-720220411-1-a7z1gi.pdf","download_url":"https://www.academia.edu/attachments/83804079/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Differential_behaviour_of_glucose_6_phos.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/83804079/0020-711x_2887_2990310-720220411-1-a7z1gi-libre.pdf?1649696593=\u0026response-content-disposition=attachment%3B+filename%3DDifferential_behaviour_of_glucose_6_phos.pdf\u0026Expires=1736936359\u0026Signature=Mvg8E~E62qX2LTwG9vvuEEUBxY3TaZoZ1bagZaqbjVe4H58peHmZ5FlfQdVOO9vRXtK9Ho3Uni~I8gXYsB7YN74LvM5Odb~YJkGYC6zV4Y0tTIEcfHpyQKf-1NQUwiCm~3AiptCTuMMCGQcVIFTZP0-4-4MkXJFqBYFNNi32xasClVQqDkl6qSzv0UARHw7ShOOpA469AZ74dlb1zTZcJ97ZDbezxQPiEpZ3CAKV7r7aUujEZQ7xOaZuo-oVMwg9ZlSEeKKH5kWd2Yaau0hJapLT6lpl7P~IMh8RqdxyzjeQ18DZtQpX1VcxDkm9w5X6VO09t9SV4iuXxKJqWctygA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Differential_behaviour_of_glucose_6_phosphate_dehydrogenase_in_two_morphological_forms_of_Trypanosoma_cruzi","translated_slug":"","page_count":5,"language":"en","content_type":"Work","summary":"Glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) of two morphological forms of Trypanosoma cruzi, epimastigotes and metacyclics, are reported. 2. 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A possible role in the carbohydrate metabolism of glucose 6-phosphate dehydrogenase in both forms of Trypanosoma cruzi is discussed.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":83804079,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/83804079/thumbnails/1.jpg","file_name":"0020-711x_2887_2990310-720220411-1-a7z1gi.pdf","download_url":"https://www.academia.edu/attachments/83804079/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Differential_behaviour_of_glucose_6_phos.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/83804079/0020-711x_2887_2990310-720220411-1-a7z1gi-libre.pdf?1649696593=\u0026response-content-disposition=attachment%3B+filename%3DDifferential_behaviour_of_glucose_6_phos.pdf\u0026Expires=1736936359\u0026Signature=Mvg8E~E62qX2LTwG9vvuEEUBxY3TaZoZ1bagZaqbjVe4H58peHmZ5FlfQdVOO9vRXtK9Ho3Uni~I8gXYsB7YN74LvM5Odb~YJkGYC6zV4Y0tTIEcfHpyQKf-1NQUwiCm~3AiptCTuMMCGQcVIFTZP0-4-4MkXJFqBYFNNi32xasClVQqDkl6qSzv0UARHw7ShOOpA469AZ74dlb1zTZcJ97ZDbezxQPiEpZ3CAKV7r7aUujEZQ7xOaZuo-oVMwg9ZlSEeKKH5kWd2Yaau0hJapLT6lpl7P~IMh8RqdxyzjeQ18DZtQpX1VcxDkm9w5X6VO09t9SV4iuXxKJqWctygA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":51531,"name":"Pergamon","url":"https://www.academia.edu/Documents/in/Pergamon"},{"id":219724,"name":"Ammonia","url":"https://www.academia.edu/Documents/in/Ammonia"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136298"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/76136298/Influence_of_some_amino_acids_on_multiplication_and_metacyclogenesis_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Influence of some amino acids on multiplication and metacyclogenesis of Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/83803933/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/76136298/Influence_of_some_amino_acids_on_multiplication_and_metacyclogenesis_of_Trypanosoma_cruzi">Influence of some amino acids on multiplication and metacyclogenesis of Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>Chemosphere</span><span>, 1990</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A two-lane extension of a recently proposed cellular automaton model for traffic flow is discusse...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A two-lane extension of a recently proposed cellular automaton model for traffic flow is discussed. The analysis focuses on the reproduction of the lane usage inversion and the density dependence of the number of lane changes. It is shown that the single-lane dynamics can be extended to the two-lane case without changing the basic properties of the model which are known to be in good agreement with empirical single-vehicle data. Therefore it is possible to reproduce various empirically observed two-lane phenomena, like the synchronization of the lanes, without fine-tuning of the model parameters.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d4690a165d1be700cb1d06b587426d69" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":83803933,"asset_id":76136298,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/83803933/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136298"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136298"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136298; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=76136298]").text(description); $(".js-view-count[data-work-id=76136298]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 76136298; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='76136298']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 76136298, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d4690a165d1be700cb1d06b587426d69" } } $('.js-work-strip[data-work-id=76136298]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":76136298,"title":"Influence of some amino acids on multiplication and metacyclogenesis of Trypanosoma cruzi","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"A two-lane extension of a recently proposed cellular automaton model for traffic flow is discussed. The analysis focuses on the reproduction of the lane usage inversion and the density dependence of the number of lane changes. It is shown that the single-lane dynamics can be extended to the two-lane case without changing the basic properties of the model which are known to be in good agreement with empirical single-vehicle data. Therefore it is possible to reproduce various empirically observed two-lane phenomena, like the synchronization of the lanes, without fine-tuning of the model parameters.","publication_date":{"day":null,"month":null,"year":1990,"errors":{}},"publication_name":"Chemosphere","grobid_abstract_attachment_id":83803933},"translated_abstract":null,"internal_url":"https://www.academia.edu/76136298/Influence_of_some_amino_acids_on_multiplication_and_metacyclogenesis_of_Trypanosoma_cruzi","translated_internal_url":"","created_at":"2022-04-11T09:38:10.214-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":15266903,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":83803933,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/83803933/thumbnails/1.jpg","file_name":"0203346.pdf","download_url":"https://www.academia.edu/attachments/83803933/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Influence_of_some_amino_acids_on_multipl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/83803933/0203346-libre.pdf?1649695886=\u0026response-content-disposition=attachment%3B+filename%3DInfluence_of_some_amino_acids_on_multipl.pdf\u0026Expires=1736936359\u0026Signature=g6PKXvpBLc5vgk03ksr3cj1HfsXK6xXYpWc0e-s3iGTCqTuIrTQHPbV1OezEjT~pcEPnovM3T0CxP1DvCev12b8tVP7d-ewQhSZIYQJD3GbWEVBxP3tJSMOOe8uDtQp2zLl79nZoyatL18trOBkxVMxanCR5SIlG5983lxbtByTVUxts2TEf6Tgg66STWLcOcml~CA7LxwTKH7AEtSIIkGCwxKxOHqPc7ib69Dp6BLNZhZLLTG5lIjGq0EpvK~Fq1qrtsXQujatoiqJhQCuGXCwvUuCy~Yy7KjB7OY0EC8ER2dnL5uht6mT2loJBSHSz6~HSYOeXq~og-6ARYAI9-w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Influence_of_some_amino_acids_on_multiplication_and_metacyclogenesis_of_Trypanosoma_cruzi","translated_slug":"","page_count":21,"language":"en","content_type":"Work","summary":"A two-lane extension of a recently proposed cellular automaton model for traffic flow is discussed. 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Therefore it is possible to reproduce various empirically observed two-lane phenomena, like the synchronization of the lanes, without fine-tuning of the model parameters.","owner":{"id":15266903,"first_name":"José Antonio","middle_initials":null,"last_name":"Lupiáñez Cara","page_name":"JoséAntonioLupiáñezCara","domain_name":"independent","created_at":"2014-08-15T23:47:07.808-07:00","display_name":"José Antonio Lupiáñez Cara","url":"https://independent.academia.edu/Jos%C3%A9AntonioLupi%C3%A1%C3%B1ezCara"},"attachments":[{"id":83803933,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/83803933/thumbnails/1.jpg","file_name":"0203346.pdf","download_url":"https://www.academia.edu/attachments/83803933/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Influence_of_some_amino_acids_on_multipl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/83803933/0203346-libre.pdf?1649695886=\u0026response-content-disposition=attachment%3B+filename%3DInfluence_of_some_amino_acids_on_multipl.pdf\u0026Expires=1736936359\u0026Signature=g6PKXvpBLc5vgk03ksr3cj1HfsXK6xXYpWc0e-s3iGTCqTuIrTQHPbV1OezEjT~pcEPnovM3T0CxP1DvCev12b8tVP7d-ewQhSZIYQJD3GbWEVBxP3tJSMOOe8uDtQp2zLl79nZoyatL18trOBkxVMxanCR5SIlG5983lxbtByTVUxts2TEf6Tgg66STWLcOcml~CA7LxwTKH7AEtSIIkGCwxKxOHqPc7ib69Dp6BLNZhZLLTG5lIjGq0EpvK~Fq1qrtsXQujatoiqJhQCuGXCwvUuCy~Yy7KjB7OY0EC8ER2dnL5uht6mT2loJBSHSz6~HSYOeXq~og-6ARYAI9-w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":51531,"name":"Pergamon","url":"https://www.academia.edu/Documents/in/Pergamon"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":585573,"name":"Amino Acid Profile","url":"https://www.academia.edu/Documents/in/Amino_Acid_Profile"}],"urls":[]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="76136279"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/76136279/Is_there_thiaminase_activity_in_Rhodnius_prolixus_Hemiptera_Reduviidae_"><img alt="Research paper thumbnail of Is there thiaminase activity in Rhodnius prolixus (Hemiptera: Reduviidae)?" class="work-thumbnail" src="https://attachments.academia-assets.com/83803928/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/76136279/Is_there_thiaminase_activity_in_Rhodnius_prolixus_Hemiptera_Reduviidae_">Is there thiaminase activity in Rhodnius prolixus (Hemiptera: Reduviidae)?</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABS'rRAcr: Studies were performed in order to elucidate if thiamine changes are related to the in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABS'rRAcr: Studies were performed in order to elucidate if thiamine changes are related to the increase of the percentage of metacyclogenesis of Trypanosoma cruzi when cultivated in Grace's medium and an extract of Rhodnius prolixus is added. A lowering of thiamine levels in a modified version of Grace's medium was confirmed. Free thiamine decreased or disappeared from the medium when the extracts were present. It is clear from the results obtained that thiamine consumption was dependent on a saturation phenomenon. Attempts to identify any enzyme activity in the et(tract of R. prolixus, which might be responsiblc for reducing thiamine levels and might also cause a similar effect in the insect's gut, thus stimulating metacyclogenesis, proved however unsuccessful. KrY Wonos: Rhodnius prolixus' Chagas'disease vector, thiamine, thiaminase activity, metacyclogenesis of Tiypanosoma cruzi. Research and Reviews in Parasitology, 53 (3-a): n7-140 (1993) O tggl Asociaci6n de Parasit6logos Espafloles</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="152750da2d304b23785f0febb0036250" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":83803928,"asset_id":76136279,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/83803928/download_file?st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&st=MTczNjkzMjc1OSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="76136279"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="76136279"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76136279; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=76136279]").text(description); $(".js-view-count[data-work-id=76136279]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 76136279; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='76136279']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 76136279, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "152750da2d304b23785f0febb0036250" } } $('.js-work-strip[data-work-id=76136279]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":76136279,"title":"Is there thiaminase activity in Rhodnius prolixus (Hemiptera: Reduviidae)?","translated_title":"","metadata":{"grobid_abstract":"ABS'rRAcr: Studies were performed in order to elucidate if thiamine changes are related to the increase of the percentage of metacyclogenesis of Trypanosoma cruzi when cultivated in Grace's medium and an extract of Rhodnius prolixus is added. 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