CINXE.COM

<!DOCTYPE html> <html lang="en" xmlns:fb="http://www.facebook.com/2008/fbml" class="wf-loading"> <head prefix="og: https://ogp.me/ns# fb: https://ogp.me/ns/fb# academia: https://ogp.me/ns/fb/academia#"> <meta charset="utf-8"> <meta name=viewport content="width=device-width, initial-scale=1"> <meta rel="search" type="application/opensearchdescription+xml" href="/open_search.xml" title="Academia.edu"> <title>RENATO MORTARA | Universidade Federal de São Paulo (UNIFESP) - Academia.edu</title>  <link href="//a.academia-assets.com/images/favicons/favicon-production.ico" rel="shortcut icon" type="image/vnd.microsoft.icon"> <link rel="apple-touch-icon" sizes="57x57" href="//a.academia-assets.com/images/favicons/apple-touch-icon-57x57.png"> <link rel="apple-touch-icon" sizes="60x60" href="//a.academia-assets.com/images/favicons/apple-touch-icon-60x60.png"> <link rel="apple-touch-icon" sizes="72x72" href="//a.academia-assets.com/images/favicons/apple-touch-icon-72x72.png"> <link rel="apple-touch-icon" sizes="76x76" href="//a.academia-assets.com/images/favicons/apple-touch-icon-76x76.png"> <link rel="apple-touch-icon" sizes="114x114" href="//a.academia-assets.com/images/favicons/apple-touch-icon-114x114.png"> <link rel="apple-touch-icon" sizes="120x120" href="//a.academia-assets.com/images/favicons/apple-touch-icon-120x120.png"> <link rel="apple-touch-icon" sizes="144x144" href="//a.academia-assets.com/images/favicons/apple-touch-icon-144x144.png"> <link rel="apple-touch-icon" sizes="152x152" href="//a.academia-assets.com/images/favicons/apple-touch-icon-152x152.png"> <link rel="apple-touch-icon" sizes="180x180" href="//a.academia-assets.com/images/favicons/apple-touch-icon-180x180.png"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-32x32.png" sizes="32x32"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-194x194.png" sizes="194x194"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-96x96.png" sizes="96x96"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/android-chrome-192x192.png" sizes="192x192"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-16x16.png" sizes="16x16"> <link rel="manifest" href="//a.academia-assets.com/images/favicons/manifest.json"> <meta name="msapplication-TileColor" content="#2b5797"> <meta name="msapplication-TileImage" content="//a.academia-assets.com/images/favicons/mstile-144x144.png"> <meta name="theme-color" content="#ffffff"> <script> window.performance && window.performance.measure && window.performance.measure("Time To First Byte", "requestStart", "responseStart"); </script> <script> (function() { if (!window.URLSearchParams || !window.history || !window.history.replaceState) { return; } var searchParams = new URLSearchParams(window.location.search); var paramsToDelete = [ 'fs', 'sm', 'swp', 'iid', 'nbs', 'rcc', // related content category 'rcpos', // related content carousel position 'rcpg', // related carousel page 'rchid', // related content hit id 'f_ri', // research interest id, for SEO tracking 'f_fri', // featured research interest, for SEO tracking (param key without value) 'f_rid', // from research interest directory for SEO tracking 'f_loswp', // from research interest pills on LOSWP sidebar for SEO tracking 'rhid', // referrring hit id ]; if (paramsToDelete.every((key) => searchParams.get(key) === null)) { return; } paramsToDelete.forEach((key) => { searchParams.delete(key); }); var cleanUrl = new URL(window.location.href); cleanUrl.search = searchParams.toString(); history.replaceState({}, document.title, cleanUrl); })(); </script> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "profiles/works", 'action': "summary", 'controller_action': 'profiles/works#summary', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script type="text/javascript"> window.sendUserTiming = function(timingName) { if (!(window.performance && window.performance.measure)) return; var entries = window.performance.getEntriesByName(timingName, "measure"); if (entries.length !== 1) return; var timingValue = Math.round(entries[0].duration); gtag('event', 'timing_complete', { name: timingName, value: timingValue, event_category: 'User-centric', }); }; window.sendUserTiming("Time To First Byte"); </script> <meta name="csrf-param" content="authenticity_token" /> <meta name="csrf-token" content="JZvvG1HjhBWx_poVkYsUVKiodeLqzjFq_NohLVxkdMT60wdAxB6mjs7Pvh-Bi_7jST47xLGBEqWACuMsHCjQwA" /> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/wow-3d36c19b4875b226bfed0fcba1dcea3f2fe61148383d97c0465c016b8c969290.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/social/home-79e78ce59bef0a338eb6540ec3d93b4a7952115b56c57f1760943128f4544d42.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/heading-95367dc03b794f6737f30123738a886cf53b7a65cdef98a922a98591d60063e3.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/button-bfbac2a470372e2f3a6661a65fa7ff0a0fbf7aa32534d9a831d683d2a6f9e01b.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/body-170d1319f0e354621e81ca17054bb147da2856ec0702fe440a99af314a6338c5.css" /><link crossorigin="" href="https://fonts.gstatic.com/" rel="preconnect" /><link href="https://fonts.googleapis.com/css2?family=DM+Sans:ital,opsz,wght@0,9..40,100..1000;1,9..40,100..1000&family=Gupter:wght@400;500;700&family=IBM+Plex+Mono:wght@300;400&family=Material+Symbols+Outlined:opsz,wght,FILL,GRAD@20,400,0,0&display=swap" rel="stylesheet" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/common-2b6f90dbd75f5941bc38f4ad716615f3ac449e7398313bb3bc225fba451cd9fa.css" /> <meta name="author" content="renato mortara" /> <meta name="description" content="Graduated in Pharmacy (USP, Brazil), MsC in Biochemistry (USP, Brazil) and PhD at the MRC Laboratory of Molecular Biology and King's College, Cambridge, UK.…" /> <meta name="google-site-verification" content="bKJMBZA7E43xhDOopFZkssMMkBRjvYERV-NaN4R6mrs" /> <script> var $controller_name = 'works'; var $action_name = "summary"; var $rails_env = 'production'; var $app_rev = 'dc2ad41da5d7ea682babd20f90650302fb0a3a36'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.Aedu = { hit_data: null }; window.Aedu.SiteStats = {"premium_universities_count":14016,"monthly_visitors":"99 million","monthly_visitor_count":99567017,"monthly_visitor_count_in_millions":99,"user_count":283003061,"paper_count":55203019,"paper_count_in_millions":55,"page_count":432000000,"page_count_in_millions":432,"pdf_count":16500000,"pdf_count_in_millions":16}; window.Aedu.serverRenderTime = new Date(1739805600000); window.Aedu.timeDifference = new Date().getTime() - 1739805600000; window.Aedu.isUsingCssV1 = false; window.Aedu.enableLocalization = true; window.Aedu.activateFullstory = false; window.Aedu.serviceAvailability = { status: {"attention_db":"on","bibliography_db":"on","contacts_db":"on","email_db":"on","indexability_db":"on","mentions_db":"on","news_db":"on","notifications_db":"on","offsite_mentions_db":"on","redshift":"on","redshift_exports_db":"on","related_works_db":"on","ring_db":"on","user_tests_db":"on"}, serviceEnabled: function(service) { return this.status[service] === "on"; }, readEnabled: function(service) { return this.serviceEnabled(service) || this.status[service] === "read_only"; }, }; window.Aedu.viewApmTrace = function() { // Check if x-apm-trace-id meta tag is set, and open the trace in APM // in a new window if it is. var apmTraceId = document.head.querySelector('meta[name="x-apm-trace-id"]'); if (apmTraceId) { var traceId = apmTraceId.content; // Use trace ID to construct URL, an example URL looks like: // https://app.datadoghq.com/apm/traces?query=trace_id%31298410148923562634 var apmUrl = 'https://app.datadoghq.com/apm/traces?query=trace_id%3A' + traceId; window.open(apmUrl, '_blank'); } }; </script>  <link href="https://fonts.googleapis.com/css?family=Roboto:100,100i,300,300i,400,400i,500,500i,700,700i,900,900i" rel="stylesheet"> <link rel="preload" href="//maxcdn.bootstrapcdn.com/font-awesome/4.3.0/css/font-awesome.min.css" as="style" onload="this.rel='stylesheet'"> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/libraries-a9675dcb01ec4ef6aa807ba772c7a5a00c1820d3ff661c1038a20f80d06bb4e4.css" /> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/academia-40698df34f913bd208bb70f09d2feb7c6286046250be17a4db35bba2c08b0e2f.css" /> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system_legacy-056a9113b9a0f5343d013b29ee1929d5a18be35fdcdceb616600b4db8bd20054.css" /> <script src="//a.academia-assets.com/assets/webpack_bundles/runtime-bundle-005434038af4252ca37c527588411a3d6a0eabb5f727fac83f8bbe7fd88d93bb.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/webpack_libraries_and_infrequently_changed.wjs-bundle-a22f75d8519394c21253dae46c8c5d60ad36ea68c7d494347ec64229d8c1cf85.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/core_webpack.wjs-bundle-5708a105dd66b4c7d0ef30b7c094b1048423f0042bd2a7b123f2d99ee3cf46d9.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/sentry.wjs-bundle-5fe03fddca915c8ba0f7edbe64c194308e8ce5abaed7bffe1255ff37549c4808.js"></script> <script> jade = window.jade || {}; jade.helpers = window.$h; jade._ = window._; </script>  <script id="tag-manager-head-root">(function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer_old','GTM-5G9JF7Z');</script>  <script> window.gptadslots = []; window.googletag = window.googletag || {}; window.googletag.cmd = window.googletag.cmd || []; </script> <script type="text/javascript"> // TODO(jacob): This should be defined, may be rare load order problem. // Checking if null is just a quick fix, will default to en if unset. // Better fix is to run this immedietely after I18n is set. if (window.I18n != null) { I18n.defaultLocale = "en"; I18n.locale = "en"; I18n.fallbacks = true; } </script> <link rel="canonical" href="https://unifesp.academia.edu/RENATOMORTARA" /> </head>   <body class='c-profiles/works a-summary logged_out'>  <div id="fb-root"></div><script>window.fbAsyncInit = function() { FB.init({ appId: "2369844204", version: "v8.0", status: true, cookie: true, xfbml: true }); // Additional initialization code. if (window.InitFacebook) { // facebook.ts already loaded, set it up. window.InitFacebook(); } else { // Set a flag for facebook.ts to find when it loads. window.academiaAuthReadyFacebook = true; } };</script><script>window.fbAsyncLoad = function() { // Protection against double calling of this function if (window.FB) { return; } (function(d, s, id){ var js, fjs = d.getElementsByTagName(s)[0]; if (d.getElementById(id)) {return;} js = d.createElement(s); js.id = id; js.src = "//connect.facebook.net/en_US/sdk.js"; fjs.parentNode.insertBefore(js, fjs); }(document, 'script', 'facebook-jssdk')); } if (!window.defer_facebook) { // Autoload if not deferred window.fbAsyncLoad(); } else { // Defer loading by 5 seconds setTimeout(function() { window.fbAsyncLoad(); }, 5000); }</script> <div id="google-root"></div><script>window.loadGoogle = function() { if (window.InitGoogle) { // google.ts already loaded, set it up. window.InitGoogle("331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b"); } else { // Set a flag for google.ts to use when it loads. window.GoogleClientID = "331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b"; } };</script><script>window.googleAsyncLoad = function() { // Protection against double calling of this function (function(d) { var js; var id = 'google-jssdk'; var ref = d.getElementsByTagName('script')[0]; if (d.getElementById(id)) { return; } js = d.createElement('script'); js.id = id; js.async = true; js.onload = loadGoogle; js.src = "https://accounts.google.com/gsi/client" ref.parentNode.insertBefore(js, ref); }(document)); } if (!window.defer_google) { // Autoload if not deferred window.googleAsyncLoad(); } else { // Defer loading by 5 seconds setTimeout(function() { window.googleAsyncLoad(); }, 5000); }</script> <div id="tag-manager-body-root">  <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-5G9JF7Z" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript>   <script> window.addEventListener('load', function() { if (document.querySelector('input[name="commit"]')) { document.querySelector('input[name="commit"]').addEventListener('click', function() { gtag('event', 'click', { event_category: 'button', event_label: 'Log In' }) }) } }); </script> </div> <script>var _comscore = _comscore || []; _comscore.push({ c1: "2", c2: "26766707" }); (function() { var s = document.createElement("script"), el = document.getElementsByTagName("script")[0]; s.async = true; s.src = (document.location.protocol == "https:" ? "https://sb" : "http://b") + ".scorecardresearch.com/beacon.js"; el.parentNode.insertBefore(s, el); })();</script><img src="https://sb.scorecardresearch.com/p?c1=2&c2=26766707&cv=2.0&cj=1" style="position: absolute; visibility: hidden" /> <div id='react-modal'></div> <div class='DesignSystem'> <a class='u-showOnFocus' href='#site'> Skip to main content </a> </div> <div id="upgrade_ie_banner" style="display: none;"><p>Academia.edu no longer supports Internet Explorer.</p><p>To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to <a href="https://www.academia.edu/upgrade-browser">upgrade your browser</a>.</p></div><script>// Show this banner for all versions of IE if (!!window.MSInputMethodContext || /(MSIE)/.test(navigator.userAgent)) { document.getElementById('upgrade_ie_banner').style.display = 'block'; }</script> <div class="DesignSystem bootstrap ShrinkableNav"><div class="navbar navbar-default main-header"><div class="container-wrapper" id="main-header-container"><div class="container"><div class="navbar-header"><div class="nav-left-wrapper u-mt0x"><div class="nav-logo"><a data-main-header-link-target="logo_home" href="https://www.academia.edu/"><img class="visible-xs-inline-block" style="height: 24px;" alt="Academia.edu" src="//a.academia-assets.com/images/academia-logo-redesign-2015-A.svg" width="24" height="24" /><img width="145.2" height="18" class="hidden-xs" style="height: 24px;" alt="Academia.edu" src="//a.academia-assets.com/images/academia-logo-redesign-2015.svg" /></a></div><div class="nav-search"><div class="SiteSearch-wrapper select2-no-default-pills"><form class="js-SiteSearch-form DesignSystem" action="https://www.academia.edu/search" accept-charset="UTF-8" method="get"><i class="SiteSearch-icon fa fa-search u-fw700 u-positionAbsolute u-tcGrayDark"></i><input class="js-SiteSearch-form-input SiteSearch-form-input form-control" data-main-header-click-target="search_input" name="q" placeholder="Search" type="text" value="" /></form></div></div></div><div class="nav-right-wrapper pull-right"><ul class="NavLinks js-main-nav list-unstyled"><li class="NavLinks-link"><a class="js-header-login-url Button Button--inverseGray Button--sm u-mb4x" id="nav_log_in" rel="nofollow" href="https://www.academia.edu/login">Log In</a></li><li class="NavLinks-link u-p0x"><a class="Button Button--inverseGray Button--sm u-mb4x" rel="nofollow" href="https://www.academia.edu/signup">Sign Up</a></li></ul><button class="hidden-lg hidden-md hidden-sm u-ml4x navbar-toggle collapsed" data-target=".js-mobile-header-links" data-toggle="collapse" type="button"><span class="icon-bar"></span><span class="icon-bar"></span><span class="icon-bar"></span></button></div></div><div class="collapse navbar-collapse js-mobile-header-links"><ul class="nav navbar-nav"><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/login">Log In</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/signup">Sign Up</a></li><li class="u-borderColorGrayLight u-borderBottom1 js-mobile-nav-expand-trigger"><a href="#">more&nbsp<span class="caret"></span></a></li><li><ul class="js-mobile-nav-expand-section nav navbar-nav u-m0x collapse"><li class="u-borderColorGrayLight u-borderBottom1"><a rel="false" href="https://www.academia.edu/about">About</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/press">Press</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="false" href="https://www.academia.edu/documents">Papers</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/terms">Terms</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/privacy">Privacy</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/copyright">Copyright</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/hiring"><i class="fa fa-briefcase"></i> We're Hiring!</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://support.academia.edu/hc/en-us"><i class="fa fa-question-circle"></i> Help Center</a></li><li class="js-mobile-nav-collapse-trigger u-borderColorGrayLight u-borderBottom1 dropup" style="display:none"><a href="#">less&nbsp<span class="caret"></span></a></li></ul></li></ul></div></div></div><script>(function(){ var $moreLink = $(".js-mobile-nav-expand-trigger"); var $lessLink = $(".js-mobile-nav-collapse-trigger"); var $section = $('.js-mobile-nav-expand-section'); $moreLink.click(function(ev){ ev.preventDefault(); $moreLink.hide(); $lessLink.show(); $section.collapse('show'); }); $lessLink.click(function(ev){ ev.preventDefault(); $moreLink.show(); $lessLink.hide(); $section.collapse('hide'); }); })() if ($a.is_logged_in() || false) { new Aedu.NavigationController({ el: '.js-main-nav', showHighlightedNotification: false }); } else { $(".js-header-login-url").attr("href", $a.loginUrlWithRedirect()); } Aedu.autocompleteSearch = new AutocompleteSearch({el: '.js-SiteSearch-form'});</script></div></div> <div id='site' class='fixed'> <div id="content" class="clearfix"> <script>document.addEventListener('DOMContentLoaded', function(){ var $dismissible = $(".dismissible_banner"); $dismissible.click(function(ev) { $dismissible.hide(); }); });</script> <script src="//a.academia-assets.com/assets/webpack_bundles/profile.wjs-bundle-c0b60aedadfb9d46b698730fbbcb2e70645c886b405d825adeba3a031c02455d.js" defer="defer"></script><script>$viewedUser = Aedu.User.set_viewed( {"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA","photo":"https://0.academia-photos.com/92671/25710/19783780/s65_renato.mortara.png","has_photo":true,"department":{"id":22107,"name":"Micro,Imuno e Parasito","url":"https://unifesp.academia.edu/Departments/Micro_Imuno_e_Parasito/Documents","university":{"id":2228,"name":"Universidade Federal de São Paulo (UNIFESP)","url":"https://unifesp.academia.edu/"}},"position":"Faculty Member","position_id":1,"is_analytics_public":false,"interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology"}]} ); if ($a.is_logged_in() && $viewedUser.is_current_user()) { $('body').addClass('profile-viewed-by-owner'); } $socialProfiles = [{"id":194118,"link":"http://www.ecb.epm.br/~ramortara","name":"Homepage","link_domain":"www.ecb.epm.br","icon":"//www.google.com/s2/u/0/favicons?domain=www.ecb.epm.br"}]</script><div id="js-react-on-rails-context" style="display:none" data-rails-context="{"inMailer":false,"i18nLocale":"en","i18nDefaultLocale":"en","href":"https://unifesp.academia.edu/RENATOMORTARA","location":"/RENATOMORTARA","scheme":"https","host":"unifesp.academia.edu","port":null,"pathname":"/RENATOMORTARA","search":null,"httpAcceptLanguage":null,"serverSide":false}"></div> <div class="js-react-on-rails-component" style="display:none" data-component-name="ProfileCheckPaperUpdate" data-props="{}" data-trace="false" data-dom-id="ProfileCheckPaperUpdate-react-component-be5db3ec-4d55-4f42-9a2c-72ad1bf28152"></div> <div id="ProfileCheckPaperUpdate-react-component-be5db3ec-4d55-4f42-9a2c-72ad1bf28152"></div> <div class="DesignSystem"><div class="onsite-ping" id="onsite-ping"></div></div><div class="profile-user-info DesignSystem"><div class="social-profile-container"><div class="left-panel-container"><div class="user-info-component-wrapper"><div class="user-summary-cta-container"><div class="user-summary-container"><div class="social-profile-avatar-container"><img class="profile-avatar u-positionAbsolute" alt="RENATO MORTARA" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/92671/25710/19783780/s200_renato.mortara.png" /></div><div class="title-container"><h1 class="ds2-5-heading-sans-serif-sm">RENATO MORTARA</h1><div class="affiliations-container fake-truncate js-profile-affiliations"><div><a class="u-tcGrayDarker" href="https://unifesp.academia.edu/">Universidade Federal de São Paulo (UNIFESP)</a>, <a class="u-tcGrayDarker" href="https://unifesp.academia.edu/Departments/Micro_Imuno_e_Parasito/Documents">Micro,Imuno e Parasito</a>, <span class="u-tcGrayDarker">Faculty Member</span></div></div></div></div><div class="sidebar-cta-container"><button class="ds2-5-button hidden profile-cta-button grow js-profile-follow-button" data-broccoli-component="user-info.follow-button" data-click-track="profile-user-info-follow-button" data-follow-user-fname="RENATO" data-follow-user-id="92671" data-follow-user-source="profile_button" data-has-google="false"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">add</span>Follow</button><button class="ds2-5-button hidden profile-cta-button grow js-profile-unfollow-button" data-broccoli-component="user-info.unfollow-button" data-click-track="profile-user-info-unfollow-button" data-unfollow-user-id="92671"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">done</span>Following</button></div></div><div class="user-stats-container"><a><div class="stat-container js-profile-followers"><p class="label">Followers</p><p class="data">122</p></div></a><a><div class="stat-container js-profile-followees" data-broccoli-component="user-info.followees-count" data-click-track="profile-expand-user-info-following"><p class="label">Following</p><p class="data">73</p></div></a><a><div class="stat-container js-profile-coauthors" data-broccoli-component="user-info.coauthors-count" data-click-track="profile-expand-user-info-coauthors"><p class="label">Co-authors</p><p class="data">56</p></div></a><div class="js-mentions-count-container" style="display: none;"><a href="/RENATOMORTARA/mentions"><div class="stat-container"><p class="label">Mentions</p><p class="data"></p></div></a></div><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div><div class="user-bio-container"><div class="profile-bio fake-truncate js-profile-about" style="margin: 0px;">Graduated in Pharmacy (USP, Brazil), MsC in Biochemistry (USP, Brazil) and PhD at the MRC Laboratory of Molecular Biology and King's College, Cambridge, UK. Full professor in Parasitology at the Division of Parasitology of the Department of Microbiology, Immunology and Parasitology of Escola Paulista de Medicina, UNIFESP, São Paulo, Brazil<br /><span class="u-fw700">Phone: </span>55 11 5579-8306<br /><b>Address: </b>Rua Botucatu, 862 6th floor<br />04069-040<br />São Paulo SP<br />Brazil<br /><div class="js-profile-less-about u-linkUnstyled u-tcGrayDarker u-textDecorationUnderline u-displayNone">less</div></div></div><div class="suggested-academics-container"><div class="suggested-academics--header"><p class="ds2-5-body-md-bold">Related Authors</p></div><ul class="suggested-user-card-list"><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://uncg.academia.edu/GwenRobbinsSchug"><img class="profile-avatar u-positionAbsolute" alt="Gwen Robbins Schug" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/161482/41323/141954071/s200_gwen.robbins_schug.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://uncg.academia.edu/GwenRobbinsSchug">Gwen Robbins Schug</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">University of North Carolina at Greensboro</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://ku-dk.academia.edu/JesperHoffmeyer"><img class="profile-avatar u-positionAbsolute" alt="Jesper Hoffmeyer" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/241162/92724/102335/s200_jesper.hoffmeyer.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://ku-dk.academia.edu/JesperHoffmeyer">Jesper Hoffmeyer</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">University of Copenhagen</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://hrepoly-ac.academia.edu/TobiasTakasShumba"><img class="profile-avatar u-positionAbsolute" alt="Tobias Takas Shumba" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/348298/314203/3319955/s200_tobias_takas.shumba.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://hrepoly-ac.academia.edu/TobiasTakasShumba">Tobias Takas Shumba</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Harare Polytechnic</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://mansoura.academia.edu/DrYouserySherif"><img class="profile-avatar u-positionAbsolute" alt="Dr.Yousery Sherif" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/445826/144163/167365/s200_yousery.sherif.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://mansoura.academia.edu/DrYouserySherif">Dr.Yousery Sherif</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Mansoura University</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://egyptfishportal.academia.edu/HanaaKader"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://egyptfishportal.academia.edu/HanaaKader">Hanaa Ahmed</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Animal Health Research Institute</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://uqar.academia.edu/RichardCloutier"><img class="profile-avatar u-positionAbsolute" alt="Richard Cloutier" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/638191/219385/421338/s200_richard.cloutier.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://uqar.academia.edu/RichardCloutier">Richard Cloutier</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Université du Québec à Rimouski</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://moscowstate.academia.edu/IrinaKolesnik"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://moscowstate.academia.edu/IrinaKolesnik">Irina Kolesnik</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Moscow State University</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://bsmrau.academia.edu/TofazzalIslam"><img class="profile-avatar u-positionAbsolute" alt="Prof. Tofazzal Islam" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/686597/235966/277690/s200_tofazzal.islam.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://bsmrau.academia.edu/TofazzalIslam">Prof. Tofazzal Islam</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Bangabandhu Sheikh Mujibur Rahman Agricultural University</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://surcollege.academia.edu/AyazMohd"><img class="profile-avatar u-positionAbsolute" alt="Dr. Ayaz Mohd" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/1779984/609285/756854/s200_ayaz.mohd.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://surcollege.academia.edu/AyazMohd">Dr. Ayaz Mohd</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">College of Applied Sciences, Oman</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://asu.academia.edu/KatieHinde"><img class="profile-avatar u-positionAbsolute" alt="Katie Hinde" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/2456953/769390/956290/s200_katie.hinde.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://asu.academia.edu/KatieHinde">Katie Hinde</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Arizona State University</p></div></div></ul></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="92671" href="https://www.academia.edu/Documents/in/Biology"><div id="js-react-on-rails-context" style="display:none" data-rails-context="{"inMailer":false,"i18nLocale":"en","i18nDefaultLocale":"en","href":"https://unifesp.academia.edu/RENATOMORTARA","location":"/RENATOMORTARA","scheme":"https","host":"unifesp.academia.edu","port":null,"pathname":"/RENATOMORTARA","search":null,"httpAcceptLanguage":null,"serverSide":false}"></div> <div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Biology"]}" data-trace="false" data-dom-id="Pill-react-component-0ce6c058-0cfc-4b71-b4ab-e902bedd07a0"></div> <div id="Pill-react-component-0ce6c058-0cfc-4b71-b4ab-e902bedd07a0"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="92671" href="https://www.academia.edu/Documents/in/Chemistry"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Chemistry"]}" data-trace="false" data-dom-id="Pill-react-component-04c12e7f-ee02-4546-9779-094f944bee01"></div> <div id="Pill-react-component-04c12e7f-ee02-4546-9779-094f944bee01"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="92671" href="https://www.academia.edu/Documents/in/Biotechnology"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Biotechnology"]}" data-trace="false" data-dom-id="Pill-react-component-f88111fb-de9c-4e42-8ac0-955cd83ebb01"></div> <div id="Pill-react-component-f88111fb-de9c-4e42-8ac0-955cd83ebb01"></div> </a></div></div><div class="external-links-container"><ul class="profile-links new-profile js-UserInfo-social"><li class="profile-profiles js-social-profiles-container"><i class="fa fa-spin fa-spinner"></i></li></ul></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by RENATO MORTARA</h3></div><div class="js-work-strip profile--work_container" data-work-id="27504140"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/27504140/Intracellular_localization_of_myeloperoxidase_in_murine_peritoneal_B_lymphocytes_and_macrophages"><img alt="Research paper thumbnail of Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages" class="work-thumbnail" src="https://attachments.academia-assets.com/47760448/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/27504140/Intracellular_localization_of_myeloperoxidase_in_murine_peritoneal_B_lymphocytes_and_macrophages">Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SabrinaOkada">Sabrina Okada</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TomazdeAraujo">Tomaz de H Araújo</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LaryssaFontoura">Laryssa Fontoura</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BAlbuquerque1">B. Albuquerque</a></span></div><div class="wp-workCard_item"><span>Cellular Immunology</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Generation of hypochlorous acid (HOCl), an important microbicidal agent, is considered to be the ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Generation of hypochlorous acid (HOCl), an important microbicidal agent, is considered to be the main function of myeloperoxidase (MPO), an enzyme present in phagocytes. High amounts of MPO are present in neutrophil azurophilic granules, which are mobilized into the phagolysosome vacuole during phagocytosis. MPO is also present in monocytes and macrophages, although to a lesser degree than in neutrophils. In the present study, we investigated the distribution of MPO in murine peritoneal cells using flow cytometry, confocal microscopy (CM) and transmission electron microscopy (TEM). MPO was observed in macrophages, and surprisingly, we detected MPO in B lymphocytes, specifically in B1-a. MPO was present in cytoplasmic granules, vesicles, mitochondria and the nucleus of murine peritoneal cells. Together, these findings suggest that, in addition to its known microbicidal activity, MPO has a myriad of other unanticipated cellular functions.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="befba512c0058510be4585f0dbf5489a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":47760448,"asset_id":27504140,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/47760448/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="27504140"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="27504140"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27504140; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27504140]").text(description); $(".js-view-count[data-work-id=27504140]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27504140; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='27504140']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "befba512c0058510be4585f0dbf5489a" } } $('.js-work-strip[data-work-id=27504140]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":27504140,"title":"Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages","internal_url":"https://www.academia.edu/27504140/Intracellular_localization_of_myeloperoxidase_in_murine_peritoneal_B_lymphocytes_and_macrophages","owner_id":51659245,"coauthors_can_edit":true,"owner":{"id":51659245,"first_name":"Sabrina","middle_initials":null,"last_name":"Okada","page_name":"SabrinaOkada","domain_name":"independent","created_at":"2016-08-03T09:08:44.642-07:00","display_name":"Sabrina Okada","url":"https://independent.academia.edu/SabrinaOkada"},"attachments":[{"id":47760448,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/47760448/thumbnails/1.jpg","file_name":"Intracellular_localization_of_myeloperox20160803-29745-oucixt.pdf","download_url":"https://www.academia.edu/attachments/47760448/download_file","bulk_download_file_name":"Intracellular_localization_of_myeloperox.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/47760448/Intracellular_localization_of_myeloperox20160803-29745-oucixt-libre.pdf?1470241149=\u0026response-content-disposition=attachment%3B+filename%3DIntracellular_localization_of_myeloperox.pdf\u0026Expires=1739809200\u0026Signature=IgssTaD~0aL3xUSJ8dxU2y8G3ssFAJ~EW8xnpRj-x-4pGL4PdSd2T25ZNcxKxwkLmJnrjGVvYav07uYP7TZClL8USDMd~AY68sf2H8lPA96rD869I0QNv0TbJwWl4fbkN9lreB0hvksRcYLdrJwv6fCt1L9Hm6g4Vf~7QjK-wcaliq9wjW-dDlVMX0OeprA0fY4lBgAz74oMG3qM8t~KX7Zg5REZE5qgLDJxiZ-LKejf90BEPL-6FNAjTOq69rU-B7PvexiYUN-HZaOx7Qsq~SBkeesfqCI6biQYWtV0v4DeDKhasOoEy8ZrG-uPHHz3viVuRGBWLe5xZQHLMlbpkQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="27504144"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/27504144/Myeloperoxidase_in_human_peripheral_blood_lymphocytes_Production_and_subcellular_localization"><img alt="Research paper thumbnail of Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/27504144/Myeloperoxidase_in_human_peripheral_blood_lymphocytes_Production_and_subcellular_localization">Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TomazdeAraujo">Tomaz de H Araújo</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SabrinaOkada">Sabrina Okada</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LaryssaFontoura">Laryssa Fontoura</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a></span></div><div class="wp-workCard_item"><span>Cellular immunology</span><span>, Jan 26, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Myeloperoxidase (MPO) is an important enzyme in the front-line protection against microorganisms....</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Myeloperoxidase (MPO) is an important enzyme in the front-line protection against microorganisms. In peripheral blood, it is accepted that MPO is only produced by myeloid-lineage cells. Thus, MPO presence is unexpected in lymphocytes. We showed recently that B1-lymphocytes from mice have MPO. Here, we showed that subsets of human peripheral B, CD4(+) and CD8(+) T lymphocytes express MPO. The content of MPO in lymphocytes was very low compared to neutrophils/monocytes with a preferential distribution in the nucleus and perinuclear region. Also, we performed a MPO mRNA expression analysis from human blood cells derived from microarray raw data publicly available, showing that MPO is modulated in infectious disease. MPO was increased in CD4(+) T lymphocytes from HIV chronic infection and in CD8(+) T lymphocytes from HCV-positive patients. Our study points out MPO as a multifunctional protein due to its subcellular localization and expression modulation in lymphocytes indicating alterna...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="27504144"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="27504144"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27504144; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27504144]").text(description); $(".js-view-count[data-work-id=27504144]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27504144; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='27504144']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=27504144]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":27504144,"title":"Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization","internal_url":"https://www.academia.edu/27504144/Myeloperoxidase_in_human_peripheral_blood_lymphocytes_Production_and_subcellular_localization","owner_id":51659245,"coauthors_can_edit":true,"owner":{"id":51659245,"first_name":"Sabrina","middle_initials":null,"last_name":"Okada","page_name":"SabrinaOkada","domain_name":"independent","created_at":"2016-08-03T09:08:44.642-07:00","display_name":"Sabrina Okada","url":"https://independent.academia.edu/SabrinaOkada"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="12637611"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/12637611/Cell_death_and_regeneration_in_the_midgut_of_the_mosquito_Culex_quinquefasciatus"><img alt="Research paper thumbnail of Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus" class="work-thumbnail" src="https://attachments.academia-assets.com/46034419/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/12637611/Cell_death_and_regeneration_in_the_midgut_of_the_mosquito_Culex_quinquefasciatus">Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/OsvaldoMarinotti">Osvaldo Marinotti</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a></span></div><div class="wp-workCard_item"><span>Journal of Insect Physiology</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Haematophagy, the utilization of blood as food, has evolved independently among insects such as m...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Haematophagy, the utilization of blood as food, has evolved independently among insects such as mosquitoes, bedbugs, fleas, and others. Accordingly, several distinct biological adaptations have occurred in order to facilitate the finding, ingestion and digestion of blood from vertebrate sources. Although blood meals are essential for survival and reproduction of these insects, mechanical and chemical stresses are caused by the ingestion of a sizable meal (frequently twice or more times the weight of the insect) containing large amounts of cytotoxic molecules such as haem. Here we present data showing that the stresses caused by a blood meal induce cell death in the midgut epithelium of Culex quinquefasciatus mosquitoes. The process involves apoptosis, ejection of dead cells to the midgut lumen and differentiation of basal regenerative cells to replace the lost digestive cells. The basal cell differentiation in blood-fed mosquito midguts represents an additional mechanism by which insects cope with the stresses caused by blood meals. C. quinquefasciatus adult females are unable to replace lost cells following a third or fourth blood meal, which may have a significant impact on mosquito longevity, reproduction and vectorial capacity. r</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a9f23997608ec7b409ba266f9127be74" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":46034419,"asset_id":12637611,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/46034419/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="12637611"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="12637611"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 12637611; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=12637611]").text(description); $(".js-view-count[data-work-id=12637611]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 12637611; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='12637611']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a9f23997608ec7b409ba266f9127be74" } } $('.js-work-strip[data-work-id=12637611]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":12637611,"title":"Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus","internal_url":"https://www.academia.edu/12637611/Cell_death_and_regeneration_in_the_midgut_of_the_mosquito_Culex_quinquefasciatus","owner_id":31609855,"coauthors_can_edit":true,"owner":{"id":31609855,"first_name":"Osvaldo","middle_initials":null,"last_name":"Marinotti","page_name":"OsvaldoMarinotti","domain_name":"independent","created_at":"2015-05-27T14:50:57.290-07:00","display_name":"Osvaldo Marinotti","url":"https://independent.academia.edu/OsvaldoMarinotti"},"attachments":[{"id":46034419,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/46034419/thumbnails/1.jpg","file_name":"Cell_death_And_regeneration_in_the_midgu20160528-14996-vakybp.pdf","download_url":"https://www.academia.edu/attachments/46034419/download_file","bulk_download_file_name":"Cell_death_and_regeneration_in_the_midgu.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/46034419/Cell_death_And_regeneration_in_the_midgu20160528-14996-vakybp-libre.pdf?1464503605=\u0026response-content-disposition=attachment%3B+filename%3DCell_death_and_regeneration_in_the_midgu.pdf\u0026Expires=1739809200\u0026Signature=GAnnDVBstvfgtOKSNYdLhABDrGOIo8IedZ~cF~8qSE3w8uXlwlDlQDzjP9AZkZiWox72-CWEtHB0MxK4FYtiCiz-khava2A8c4j11or1Fopth5w03FlAwu7ZPc9ozc6rPnfo~WQNgQWq9~YTzeArBtsJ3iccEcIE02tm1grjYG3-SmefPvQucrT68iCCmtYwJpZtyKxgzqyxTp0R85uSR1NFyqrPemNYh7yzPLASSqyk4J~Bt~QwSOIMy1DOeMJ5kuJh1qhhCchfodFYiceFEBLjhLwQ1Q3Vi8vBjbRlszICm5~tWAwITTqgQD794DM6SbSn-SHBjU8n4Yg1WSO1MA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="24946260"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/24946260/Trypanosoma_cruzi_single_cell_live_imaging_inside_infected_tissues"><img alt="Research paper thumbnail of Trypanosoma cruzi: single cell live imaging inside infected tissues" class="work-thumbnail" src="https://attachments.academia-assets.com/45277126/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/24946260/Trypanosoma_cruzi_single_cell_live_imaging_inside_infected_tissues">Trypanosoma cruzi: single cell live imaging inside infected tissues</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/EstebanCordero1">Esteban Cordero</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RMortara">Renato Mortara</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Although imaging the live Trypanosoma cruzi parasite is a routine technique in most laboratories,...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Although imaging the live Trypanosoma cruzi parasite is a routine technique in most laboratories, identification of the parasite in infected tissues and organs has been hindered by their intrinsic opaque nature. We describe a simple method for in vivo observation of live single cell Trypanosoma cruzi parasites inside mammalian host tissues. BALB/c or C57BL/6 mice infected with DsRed-CL or GFP-G trypomastigotes had their organs removed and sectioned with surgical blades. Ex vivo organ sections were observed under confocal microscopy. For the first time, this procedure enabled imaging of individual amastigotes, intermediate forms and motile trypomastigotes within infected tissues of mammalian hosts.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="34a420d7161e34a4648e6e36d4734f4a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45277126,"asset_id":24946260,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45277126/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24946260"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24946260"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24946260; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24946260]").text(description); $(".js-view-count[data-work-id=24946260]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24946260; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='24946260']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "34a420d7161e34a4648e6e36d4734f4a" } } $('.js-work-strip[data-work-id=24946260]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":24946260,"title":"Trypanosoma cruzi: single cell live imaging inside infected tissues","internal_url":"https://www.academia.edu/24946260/Trypanosoma_cruzi_single_cell_live_imaging_inside_infected_tissues","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":45277126,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45277126/thumbnails/1.jpg","file_name":"Ferreira_Cell_Microbiology.pdf","download_url":"https://www.academia.edu/attachments/45277126/download_file","bulk_download_file_name":"Trypanosoma_cruzi_single_cell_live_imagi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45277126/Ferreira_Cell_Microbiology-libre.pdf?1462197345=\u0026response-content-disposition=attachment%3B+filename%3DTrypanosoma_cruzi_single_cell_live_imagi.pdf\u0026Expires=1739809200\u0026Signature=KGU~8mh048HMp2rhizv0bF1j4gwdUqPo53M4B03VhrmtkjOyAku0SQJELll2F7I8WIHmleXkpCm36imugQIMtIuNpMs0USmmCdv--z4qSSBj6cd-3U-yJcsKpm8D5BE7cKYl5HxdnvpaDlycRfcFDMP5M5rKK2XNA6ekBTL1JI59RxrPjXyDdTYJLxnqLk2a~TwGBTdG-dNCg~P9NolL25fXso4dNuvrwY3~oeKgK1WJ53eoRtmHUaTnQtUQbQwMomlSoE-c~wfhXQ3jmVxVWrwxMYccI23aeivtz3ej74fkrFoG-udCz8gOReu6MxqOlsgNokXUEwqhSv~CnkggMA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="23663028"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/23663028/Ultrastructural_and_cytochemical_identification_of_megasome_in_Leishmania_Leishmania_chagasi"><img alt="Research paper thumbnail of Ultrastructural and cytochemical identification of megasome in Leishmania (Leishmania) chagasi" class="work-thumbnail" src="https://attachments.academia-assets.com/44069638/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/23663028/Ultrastructural_and_cytochemical_identification_of_megasome_in_Leishmania_Leishmania_chagasi">Ultrastructural and cytochemical identification of megasome in Leishmania (Leishmania) chagasi</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uepa.academia.edu/SannyAlberio">Sanny Alberio</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a></span></div><div class="wp-workCard_item"><span>Parasitology Research</span><span>, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The present work showed the presence of a megasome in Leishmania (Leishmania) chagasi amastigotes...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The present work showed the presence of a megasome in Leishmania (Leishmania) chagasi amastigotes. Transmission electron microscopy analysis of ultrathin serial sections and three-dimensional reconstruction allowed visualization of large structures in amastigote forms of L. (L.) chagasi and a multivesicular tubule-lysosome structure in metacyclic promastigotes. Morphometric data showed that the relative volume occupied by the megasome and the multivesicular tubule (MVT)-lysosome structures was about 5% and 3.2%, respectively, in amastigotes and promastigotes of L. (L.) chagasi. Further characterization of the megasome in L. (L.) chagasi amastigotes was carried out by immunolabeling of cysteine proteinase, whereas the lysosomal content of amastigotes and promastigotes was confirmed by arylsulfatase cytochemistry.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cf6d4fca172c1e0102da7851a11f69fa" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44069638,"asset_id":23663028,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44069638/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="23663028"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="23663028"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23663028; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23663028]").text(description); $(".js-view-count[data-work-id=23663028]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23663028; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='23663028']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cf6d4fca172c1e0102da7851a11f69fa" } } $('.js-work-strip[data-work-id=23663028]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":23663028,"title":"Ultrastructural and cytochemical identification of megasome in Leishmania (Leishmania) chagasi","internal_url":"https://www.academia.edu/23663028/Ultrastructural_and_cytochemical_identification_of_megasome_in_Leishmania_Leishmania_chagasi","owner_id":45723431,"coauthors_can_edit":true,"owner":{"id":45723431,"first_name":"Sanny","middle_initials":null,"last_name":"Alberio","page_name":"SannyAlberio","domain_name":"uepa","created_at":"2016-03-24T07:49:40.951-07:00","display_name":"Sanny Alberio","url":"https://uepa.academia.edu/SannyAlberio"},"attachments":[{"id":44069638,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/44069638/thumbnails/1.jpg","file_name":"Alberio-2004.pdf","download_url":"https://www.academia.edu/attachments/44069638/download_file","bulk_download_file_name":"Ultrastructural_and_cytochemical_identif.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/44069638/Alberio-2004-libre.pdf?1458831393=\u0026response-content-disposition=attachment%3B+filename%3DUltrastructural_and_cytochemical_identif.pdf\u0026Expires=1739809200\u0026Signature=gD3FMHgJucg1eeJH4R1lPlAd8ef4gXlToAwYdRRuSf5HZ5Fju0b~FrdHi6sxuFz9rOfKPOAc4-xbP3PTxw9Z2qCQA6~GEtr32HBCkTABKUmaWVcKEE-g44STAmkjopYvORQole~ZCR4-nVB5hKi1cyRYq81970SqKVOL0ZP3SfjOopJZV7Us7Vbsn~rih~NgY6jMiAaUfIaTlk0~AWoNlimD9MaThRQYghVMizQqsb~QoTYmD9hHY352M0nFRMFGsZsuWP79vU0D79KXo0y6JCmQQ5-Zzjfh6G4ERXFBj9yqAXU56zEEMyi6OfrSEP-SI9lNl11oPU1yf6lt5L81xg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503044"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503044/Revealing_Annexin_A2_and_ARF_6_enrollment_during_Trypanosoma_cruzi_extracellular_amastigote_host_cell_interaction"><img alt="Research paper thumbnail of Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction" class="work-thumbnail" src="https://attachments.academia-assets.com/41407527/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503044/Revealing_Annexin_A2_and_ARF_6_enrollment_during_Trypanosoma_cruzi_extracellular_amastigote_host_cell_interaction">Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction</a></div><div class="wp-workCard_item"><span>Parasites & vectors</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerizatio...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerization-dependent. However, the role of proteins related to actin dynamics during invasion by amastigotes remains to be investigated. Here we describe the role of Annexin A2 and ARF-6 during extracellular amastigote-mammalian cell interactions. Our results showed ARF-6 accumulation in the amastigote-containing parasitophorous vacuole containing amastigote forms; demonstrated ARF-6 and Annexin A2 critical impact over parasite cell invasion and revealed the effect of Annexin A2 expression on intracellular parasite multiplication. ARF-6 and Annexin A2 are involved in invasion of mammalian cells by T. cruzi amastigotes.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="77c916570ef0a4a87175514f76329645" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407527,"asset_id":20503044,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407527/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503044"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503044"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503044; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503044]").text(description); $(".js-view-count[data-work-id=20503044]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503044; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503044']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "77c916570ef0a4a87175514f76329645" } } $('.js-work-strip[data-work-id=20503044]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503044,"title":"Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction","internal_url":"https://www.academia.edu/20503044/Revealing_Annexin_A2_and_ARF_6_enrollment_during_Trypanosoma_cruzi_extracellular_amastigote_host_cell_interaction","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407527,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407527/thumbnails/1.jpg","file_name":"563cee0308ae45b5d289962a.pdf","download_url":"https://www.academia.edu/attachments/41407527/download_file","bulk_download_file_name":"Revealing_Annexin_A2_and_ARF_6_enrollmen.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407527/563cee0308ae45b5d289962a-libre.pdf?1453458874=\u0026response-content-disposition=attachment%3B+filename%3DRevealing_Annexin_A2_and_ARF_6_enrollmen.pdf\u0026Expires=1739809200\u0026Signature=P7xSSMFQ1D79cL-cwoPA4Nr7125R8Awz3~yalTqHbUgm9skriov-aZsLCcxlRttka-tRKq9qBU9M0HhtoTcMLC34DyFUbBhTPYk22IoDLVqOm-7PfX6k7rGFMQFcix8rAffCn6BC-tXR6kZF23PTFprP~JrOTfEy6TC~YSjffdfmLTdZ2KqdH69E~vBjHXAwhf9CN95IHO8-uzXU-sshgCdXP9OZwgu7fxmA7gYx9RSL4bHIyBu95bexQ7lnXYDYderbOmJP1RIgtFPTCu-lqGQbVQNsu-bgTc-O-f5BnTxZeNWzoU2vLOr7liky9pKZgAxIfnjLqHeVyeIYWn96bw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503042"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/20503042/Recruitment_of_PH_AKT_to_nascent_phagosomes_containing_T_cruzi_G_strain_EAs"><img alt="Research paper thumbnail of Recruitment of PH-AKT to nascent phagosomes containing T. cruzi G strain EAs" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/20503042/Recruitment_of_PH_AKT_to_nascent_phagosomes_containing_T_cruzi_G_strain_EAs">Recruitment of PH-AKT to nascent phagosomes containing T. cruzi G strain EAs</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503042"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503042"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503042; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503042]").text(description); $(".js-view-count[data-work-id=20503042]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503042; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503042']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=20503042]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503042,"title":"Recruitment of PH-AKT to nascent phagosomes containing T. cruzi G strain EAs","internal_url":"https://www.academia.edu/20503042/Recruitment_of_PH_AKT_to_nascent_phagosomes_containing_T_cruzi_G_strain_EAs","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503041"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503041/Phosphatidylinositol_Specific_Phospholipase_C_PI_PLC_Cleavage_of_GPI_Anchored_Surface_Molecules_of_Trypanosoma_cruzi_Triggers_In_Vitro_Morphological_Reorganization_of_Trypomastigotes"><img alt="Research paper thumbnail of Phosphatidylinositol‐Specific Phospholipase C (PI‐PLC) Cleavage of GPI‐Anchored Surface Molecules of Trypanosoma cruzi Triggers In Vitro Morphological Reorganization of Trypomastigotes" class="work-thumbnail" src="https://attachments.academia-assets.com/41407688/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503041/Phosphatidylinositol_Specific_Phospholipase_C_PI_PLC_Cleavage_of_GPI_Anchored_Surface_Molecules_of_Trypanosoma_cruzi_Triggers_In_Vitro_Morphological_Reorganization_of_Trypomastigotes">Phosphatidylinositol‐Specific Phospholipase C (PI‐PLC) Cleavage of GPI‐Anchored Surface Molecules of Trypanosoma cruzi Triggers In Vitro Morphological Reorganization of Trypomastigotes</a></div><div class="wp-workCard_item"><span>Journal of Eukaryotic Microbiology</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Trypanosoma cruzi trypomastigotes treated with phosphatidylinositol-specific phospholipase C (PI-...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Trypanosoma cruzi trypomastigotes treated with phosphatidylinositol-specific phospholipase C (PI-PLC) in vitro are rapidly induced to differentiate into round forms. Using confocal microscopy, we were able to show that trypomastigotes treated with PI-PLC initiate the process of flagellum remodeling by 30 sec after contact with the enzyme and amastigote-like forms are detected as early as 10 min after PI-PLC treatment. Scanning and transmission electron microscopy indicate that trypomastigotes undergo a previously undescribed process of flagellum circularization and internalization. Analysis of the flagellar complex with monoclonal antibody 4D9 shows heterogeneous labeling among the parasites, suggesting a remodeling of these molecules. After PI-PLC treatment, parasites rapidly lose the surface marker Ssp-3 and 24 h post-treatment they begin to exhibit a circular nucleus and a rod-shaped kinetoplast. By flow cytometry analysis and confocal microscopy, the Ssp-4 amastigote-specific ep...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dec4adb7c9e77623c27d1e954648a710" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407688,"asset_id":20503041,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407688/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503041"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503041"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503041; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503041]").text(description); $(".js-view-count[data-work-id=20503041]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503041; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503041']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "dec4adb7c9e77623c27d1e954648a710" } } $('.js-work-strip[data-work-id=20503041]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503041,"title":"Phosphatidylinositol‐Specific Phospholipase C (PI‐PLC) Cleavage of GPI‐Anchored Surface Molecules of Trypanosoma cruzi Triggers In Vitro Morphological Reorganization of Trypomastigotes","internal_url":"https://www.academia.edu/20503041/Phosphatidylinositol_Specific_Phospholipase_C_PI_PLC_Cleavage_of_GPI_Anchored_Surface_Molecules_of_Trypanosoma_cruzi_Triggers_In_Vitro_Morphological_Reorganization_of_Trypomastigotes","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407688,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407688/thumbnails/1.jpg","file_name":"PhosphatidylinositolSpecific_Phospholipa20160122-30106-63ircd.pdf","download_url":"https://www.academia.edu/attachments/41407688/download_file","bulk_download_file_name":"Phosphatidylinositol_Specific_Phospholip.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407688/PhosphatidylinositolSpecific_Phospholipa20160122-30106-63ircd-libre.pdf?1453458908=\u0026response-content-disposition=attachment%3B+filename%3DPhosphatidylinositol_Specific_Phospholip.pdf\u0026Expires=1739809200\u0026Signature=YcPxb-LyTjkW8XBHd8N6r67GUsWESu9uDe~Tnk5jGYvT~3YBHFLS5pFMqqV3OqlHkt5gUicTStjNsdE7BeOEHuY75DQ-hThYzKfC~kJaWVrj03O79jMWILSRb~UC-PJNh~nVwzBJXxkoUwxggHCCU8BESer-oSwR1iD5TA7V7gkm~a9eLkdbATuKPQIsccO6ZFIq~Z8dTH4owHvhe7b8Jksi8H6InDkXocK7AcgfSY2L5-53A3V~U6Pkn63RAID5OVLm48~Gy7IyZ07t3rk~rU3VNw-rx-C3i8-uOnArT6zgkE4g8Oe0fp-bDvh4ao70CTsrSHqtkiOm2id2lsiIeA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503039"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503039/Detection_and_localization_of_small_nuclear_ribonucleoproteins_snRNPs_in_trypanosomatids_using_anti_m3G_antibodies"><img alt="Research paper thumbnail of Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies" class="work-thumbnail" src="https://attachments.academia-assets.com/41407526/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503039/Detection_and_localization_of_small_nuclear_ribonucleoproteins_snRNPs_in_trypanosomatids_using_anti_m3G_antibodies">Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies</a></div><div class="wp-workCard_item"><span>Japanese journal of infectious diseases</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This work reports for the first time the identification and immunolocalization, by confocal and c...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This work reports for the first time the identification and immunolocalization, by confocal and conventional indirect immunofluorescence, of m3G epitopes present in ribonucleoproteins of the following trypanosomatids: Trypanosoma cruzi epimastigotes of three different strains, Blastocrithidia ssp., and Leishmania major promastigotes. The identity of these epitopes and hence the specificity of the anti-m3G monoclonal antibody were ascertained through competition reaction with 7-methylguanosine that blocks the Ig binding sites, abolishing the fluorescence in all the parasites tested and showing a specific perinuclear localization of the snRNPs, which suggests their nuclear reimport in the parasites. Using an immunoprecipitation technique, it was also possible to confirm the presence of the trimethylguanosine epitopes in trypanosomatids.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9a4254e72c45becf13d650b4a98605e5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407526,"asset_id":20503039,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407526/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503039"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503039"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503039; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503039]").text(description); $(".js-view-count[data-work-id=20503039]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503039; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503039']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9a4254e72c45becf13d650b4a98605e5" } } $('.js-work-strip[data-work-id=20503039]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503039,"title":"Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies","internal_url":"https://www.academia.edu/20503039/Detection_and_localization_of_small_nuclear_ribonucleoproteins_snRNPs_in_trypanosomatids_using_anti_m3G_antibodies","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407526,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407526/thumbnails/1.jpg","file_name":"Bosetto-2008.pdf","download_url":"https://www.academia.edu/attachments/41407526/download_file","bulk_download_file_name":"Detection_and_localization_of_small_nucl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407526/Bosetto-2008-libre.pdf?1453458874=\u0026response-content-disposition=attachment%3B+filename%3DDetection_and_localization_of_small_nucl.pdf\u0026Expires=1739809200\u0026Signature=fwz3dO0~uivZ219GDgag57pXNFK0b-Tg5rNnz68xWMaKcIIPc7u~KbcnAC3vk2rw71-VFRblIfhSdvo4J0dvaO1wMnYVvJzbZkUrpFGirkRAFo5cxtclyZn30KJlCNwqt2CM7YXNwzdk3FWNN3WYNgenam0XvMpK5ho~vjVfNPGFC8Y3hq8IkiVBkZqY4VF~MTgeUxWfLrc1Tk9EhF8SBo1AyMrgnJI6ItX0VhHChBgu9aWQIxjt8G~DztF8bc1zVTXTIZ8qqhe9zeegMHaOeGs7a12YOMycKS2C-K070bUrrIrcSXI73lJ~oWVLLmcLXPP~Qj3seIKRejtwYagvlw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503036"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503036/Polymorphism_ofthe35_and50_Kilodalton_Surface_Glycoconjugates_ofTrypanosoma_cnrzi_Metacyclic_Trypomastigotes"><img alt="Research paper thumbnail of Polymorphism ofthe35-and50-Kilodalton Surface Glycoconjugates ofTrypanosoma cnrzi Metacyclic Trypomastigotes" class="work-thumbnail" src="https://attachments.academia-assets.com/41407689/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503036/Polymorphism_ofthe35_and50_Kilodalton_Surface_Glycoconjugates_ofTrypanosoma_cnrzi_Metacyclic_Trypomastigotes">Polymorphism ofthe35-and50-Kilodalton Surface Glycoconjugates ofTrypanosoma cnrzi Metacyclic Trypomastigotes</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In this study, we examined the immunochemical properties of the 35and 50-kDa (35/50-kDa) surface ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In this study, we examined the immunochemical properties of the 35and 50-kDa (35/50-kDa) surface glycoconjugates expressed on the surface of metacyclic trypomastigotes of Trypanosoma cruzi using three different monoclonal antibodies directed to this component. The 35/50-kDa surface antigen was expressed by metacycic trypomastigotes of 11 different strains of T. cruzi and displayed a significant degree of molecular polymorphism. Results of immunoblotting and complement-mediated lysis were consistent with such diversity. Different monoclonal antibodies reacted with distinct epitopes on the 35/50-kDa antigen, which is resistant to proteases and behaves as an amphiphilic component.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0c519c62169fef7c9a4df7c601310767" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407689,"asset_id":20503036,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407689/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503036"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503036"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503036; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503036]").text(description); $(".js-view-count[data-work-id=20503036]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503036; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503036']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0c519c62169fef7c9a4df7c601310767" } } $('.js-work-strip[data-work-id=20503036]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503036,"title":"Polymorphism ofthe35-and50-Kilodalton Surface Glycoconjugates ofTrypanosoma cnrzi Metacyclic Trypomastigotes","internal_url":"https://www.academia.edu/20503036/Polymorphism_ofthe35_and50_Kilodalton_Surface_Glycoconjugates_ofTrypanosoma_cnrzi_Metacyclic_Trypomastigotes","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407689,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407689/thumbnails/1.jpg","file_name":"Polymorphism_ofthe35-and50-Kilodalton_Su20160122-17380-1ryipy0.pdf","download_url":"https://www.academia.edu/attachments/41407689/download_file","bulk_download_file_name":"Polymorphism_ofthe35_and50_Kilodalton_Su.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407689/Polymorphism_ofthe35-and50-Kilodalton_Su20160122-17380-1ryipy0-libre.pdf?1453458909=\u0026response-content-disposition=attachment%3B+filename%3DPolymorphism_ofthe35_and50_Kilodalton_Su.pdf\u0026Expires=1739809200\u0026Signature=SgVqm02hftxO0dyHCKlG~1WNe69NtBUreqAzt5t-Gp~qt5FRt0zLVdbnlLjrrUIg5SphN9LzkEUvV-LhEn-0q1HwoPQE20R~4T744hDghuVVS3ZBwQNqWVoi8Tat0kXSJZawy6~-O~r35N8Jz6MXvqyrWTLdZoAAH0ZRTcHT1F5r-MDojjHPN9KgZvIQ~1QDbdHvXQawspjshe-8kAPh3lF9iPGyu41ywmhnXu1PZvksj4BuxRSUfynuiyTjU-lTmPw4zB3mN933KKcP3cRBn6Is84fqc4ba43OEmDDTlcttCUyxpPagNQjL1ohvYbtk1IcguLB9bdHwt0-LIdfSLA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503034"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503034/Central_nervous_system_involvement_in_experimental_infection_with_Leishmania_Leishmania_amazonensis"><img alt="Research paper thumbnail of Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis" class="work-thumbnail" src="https://attachments.academia-assets.com/41407516/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503034/Central_nervous_system_involvement_in_experimental_infection_with_Leishmania_Leishmania_amazonensis">Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis</a></div><div class="wp-workCard_item"><span>The American journal of tropical medicine and hygiene</span><span>, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We describe the pathologic alterations of the central nervous system (CNS) observed in experiment...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We describe the pathologic alterations of the central nervous system (CNS) observed in experimental tegumentary leishmaniasis in BALB/c and Swiss mice. The mice were subcutaneously infected with 10(4) amastigotes of Leishmania (Leishmania) amazonensis. Animals were killed and brains were removed for histologic and immunocytochemical studies. Histologic examination showed that 66.6% of infected mice had a discrete hyperemia and inflammatory infiltrate in the meninges, composed of mononuclear cells and neutrophils with no detectable parasites. However, parasitized macrophages were detected in the cerebral parenchyma, as well as mast cells, lymphocytes, and polymorphonuclear cells. Necrosis in the cerebral parenchyma was also observed. Confocal fluorescence microscopy showed that CD8+ T lymphocytes are the major component of the inflammatory infiltrate in the CNS. In addition to these cells, CD4+, CD11b, and dendritic cells are present, in small numbers, in the inflammatory processes o...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7ef5b83a552453af2a5137f00f8d44db" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407516,"asset_id":20503034,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407516/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503034"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503034"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503034; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503034]").text(description); $(".js-view-count[data-work-id=20503034]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503034; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503034']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7ef5b83a552453af2a5137f00f8d44db" } } $('.js-work-strip[data-work-id=20503034]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503034,"title":"Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis","internal_url":"https://www.academia.edu/20503034/Central_nervous_system_involvement_in_experimental_infection_with_Leishmania_Leishmania_amazonensis","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407516,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407516/thumbnails/1.jpg","file_name":"661.pdf","download_url":"https://www.academia.edu/attachments/41407516/download_file","bulk_download_file_name":"Central_nervous_system_involvement_in_ex.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407516/661-libre.pdf?1453458875=\u0026response-content-disposition=attachment%3B+filename%3DCentral_nervous_system_involvement_in_ex.pdf\u0026Expires=1739809200\u0026Signature=DlHrr1FsyfjLTWNy9iT5dnKUmMqVWfhtQbQz-ajLRFnSg60W0DZm2-tAUBUbeG8fZ3jUx1OKyw1UEa0aJbP3wxSxiistN-FIBETHUw9MkObsuH1xQOZLhzdQU9~BwJm1xbiVsa7f839X4NRDwKn3E-LJW0LSTZJL-ukKZOkVynjRiO8TqaYNb4wFKD-qyr61FlICj-3QcxtP6He10E6vYhc4YUszPkX9F~-cR-rgsLHSU3CE8OyVtnq42JRu-eF6Jnn~8JH~~w5lHTaW-eHKKh2XaK~nK1w9O2EvqUZHevwFt4owptnOpQ3efsF3w1sC9UuzWauQ~kl-Esy2nfhNNg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503032"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503032/Electrophoretic_karyotypes_and_genome_sizing_of_the_pathogenic_fungus_Paracoccidioides_brasiliensis"><img alt="Research paper thumbnail of Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis" class="work-thumbnail" src="https://attachments.academia-assets.com/41407513/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503032/Electrophoretic_karyotypes_and_genome_sizing_of_the_pathogenic_fungus_Paracoccidioides_brasiliensis">Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis</a></div><div class="wp-workCard_item"><span>Journal of clinical microbiology</span><span>, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Here we present the karyotype analysis and genome sizing of Paracoccidioides brasiliensis, a path...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Here we present the karyotype analysis and genome sizing of Paracoccidioides brasiliensis, a pathogen refractory to conventional genetic analysis. We have established pulsed-field gel electrophoresis (PFGE) conditions to resolve the high-molecular-weight chromosomal bands of two clinical isolates of P. brasiliensis. Both isolates showed four megabase-sized bands, ranging from 2.0 to 10.0 Mbp. Significant differences in chromosome sizes and in the chromosomal location of genes for the gp43 antigen and chitin synthase were found. Different technical approaches were employed to estimate the DNA content and to define the ploidy of P. brasiliensis. An estimated genome size in the range of 45.7 to 60.9 Mbp was provided by the analysis of data generated by measuring the amplitude of fluorescence intensity of DAPI (4&#39;,6-diamidino-2-phenylindole)-stained nuclei (by confocal microscopy). The nuclear genome size estimated by confocal microscopy is twice that estimated by the average sum of...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="90a5e177bea4b6fabae5ea26661e6fc0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407513,"asset_id":20503032,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407513/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503032"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503032"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503032; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503032]").text(description); $(".js-view-count[data-work-id=20503032]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503032; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503032']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "90a5e177bea4b6fabae5ea26661e6fc0" } } $('.js-work-strip[data-work-id=20503032]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503032,"title":"Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis","internal_url":"https://www.academia.edu/20503032/Electrophoretic_karyotypes_and_genome_sizing_of_the_pathogenic_fungus_Paracoccidioides_brasiliensis","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407513,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407513/thumbnails/1.jpg","file_name":"Cano-1998.pdf","download_url":"https://www.academia.edu/attachments/41407513/download_file","bulk_download_file_name":"Electrophoretic_karyotypes_and_genome_si.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407513/Cano-1998-libre.pdf?1453458875=\u0026response-content-disposition=attachment%3B+filename%3DElectrophoretic_karyotypes_and_genome_si.pdf\u0026Expires=1739809200\u0026Signature=J4X2-kiNEy4Ffb2HaX2AFjwBkWkFVDWslThDNvgghgpytfli3bQuzcaHqDbZI~8m9WUedc3VZDySnsXySGb5-X5evW8jS0gPuWAY9-mIc46ApgW-4v2klj74S~RkNwQnFwtUjX4EbyFiJj0Cob4Q0NXboIiMEphhY0HzGM9T0SW7qX-ce8Wu4TAqpvA7Btz9AtS5t0gZGyw0js0HrJmkiZSWmj8rb3QSp~PtmdXixVIe3vdVc6GPQu8vhA8x4UsT1-OlmGfcmPsXA0SQPcerKwuG-bPdDuZi4usESovnDYBzFlfybSPXScNavDbLpw0pCzavpDPoiyNeYk4D-ElSVQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503030"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503030/Endoplasmic_reticulum_contains_a_common_abundant_calcium_binding_glycoprotein_endoplasmin"><img alt="Research paper thumbnail of Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin" class="work-thumbnail" src="https://attachments.academia-assets.com/41407530/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503030/Endoplasmic_reticulum_contains_a_common_abundant_calcium_binding_glycoprotein_endoplasmin">Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin</a></div><div class="wp-workCard_item"><span>Journal of cell science</span><span>, 1986</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The most abundant protein in microsomal membrane preparations from mammalian cells has been ident...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The most abundant protein in microsomal membrane preparations from mammalian cells has been identified as a 100 X 10(3) Mr concanavalin A-binding glycoprotein. The glycosyl moiety of the glycoprotein is completely sensitive to endoglycosidase H, suggesting a predominantly endoplasmic reticulum localization in the cell. Using a monospecific antibody it was shown by binding and immunofluorescence studies that the glycoprotein is intracellular. Immunoelectron microscopy showed that the glycoprotein was at least 100 times more concentrated in the endoplasmic reticulum than in any other cellular organelle. It was found to be substantially overexpressed in cells and tissues rich in endoplasmic reticulum. Since it is the major common protein component associated with the endoplasmic reticulum we refer to it as endoplasmin. Calcium-binding studies show that endoplasmin is a major calcium-binding protein in cells, suggesting that at least one of its roles might be in the calcium-storage func...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fff614aae6ec6a0e49e0c41045dc4bb0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407530,"asset_id":20503030,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407530/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503030"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503030"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503030; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503030]").text(description); $(".js-view-count[data-work-id=20503030]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503030; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503030']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fff614aae6ec6a0e49e0c41045dc4bb0" } } $('.js-work-strip[data-work-id=20503030]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503030,"title":"Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin","internal_url":"https://www.academia.edu/20503030/Endoplasmic_reticulum_contains_a_common_abundant_calcium_binding_glycoprotein_endoplasmin","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407530,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407530/thumbnails/1.jpg","file_name":"Endoplasmic_reticulum_contains_a_common_20160122-6217-14ilvzh.pdf","download_url":"https://www.academia.edu/attachments/41407530/download_file","bulk_download_file_name":"Endoplasmic_reticulum_contains_a_common.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407530/Endoplasmic_reticulum_contains_a_common_20160122-6217-14ilvzh-libre.pdf?1453458877=\u0026response-content-disposition=attachment%3B+filename%3DEndoplasmic_reticulum_contains_a_common.pdf\u0026Expires=1739809200\u0026Signature=Ma2Ba1KV0gYYnspD8Pmzj5KVaVp1QZT8Wd3kBm3CbRTpnlz2HH-BxhPVNqcf7ibcJvtSrn3eWS0eEyeUH2e71shFeV6G19pVobf9ij1laKHr3UsaT3lMN-ugfBR-nR2TfCaqafTr~wUaxL8zhyKb1x8D2u5GF-ef7V52euMQoZ2-zuYcirSr3L2Fk8D6qNuQ12iuxx8q29IK9~JKIt5auwvVNOidFFJxRuQwNDsUqHbLcyoyh6AHvfYIds2jQoanG3fMy96obxzhn8u41YRwS4kSGTjXIMhTljvDXcHI9sNdzkoHISkXtp0lR1utBtn7s5tIwd65D4OIa7PHJ-vqxg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503027"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503027/An_association_between_actin_and_nucleocapsid_polypeptides_in_isolated_murine_retroviral_particles"><img alt="Research paper thumbnail of An association between actin and nucleocapsid polypeptides in isolated murine retroviral particles" class="work-thumbnail" src="https://attachments.academia-assets.com/41407528/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503027/An_association_between_actin_and_nucleocapsid_polypeptides_in_isolated_murine_retroviral_particles">An association between actin and nucleocapsid polypeptides in isolated murine retroviral particles</a></div><div class="wp-workCard_item"><span>Journal of submicroscopic cytology and pathology</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mammalian cells infected with retroviruses frequently display virus particles budding at the tip ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mammalian cells infected with retroviruses frequently display virus particles budding at the tip of cellular projections resembling microvilli and filopodia. In normal and infected cells these cellular projections contain actin microfilaments and in specialized retrovirus-tipped projections from the P815 cell, a direct association between actin filaments and the apical virus particle could be demonstrated (Mortara and Koch, 1986). Here we confirm and extend these observations using a murine macrophage cell line chronically infected with a C-type retrovirus. Immunochemical and biochemical methods were used to identify actin-associated and actin-binding components among the retroviral polypeptides. The results show that Pr65gag and its p15 N-terminal domain can bind to actin in vitro and may be major binding sites for actin filaments on the retroviral nucleocapsid.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="665af9f0099f25fe8e36c9420c3c5668" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407528,"asset_id":20503027,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407528/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503027"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503027"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503027; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503027]").text(description); $(".js-view-count[data-work-id=20503027]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503027; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503027']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "665af9f0099f25fe8e36c9420c3c5668" } } $('.js-work-strip[data-work-id=20503027]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503027,"title":"An association between actin and nucleocapsid polypeptides in isolated murine retroviral particles","internal_url":"https://www.academia.edu/20503027/An_association_between_actin_and_nucleocapsid_polypeptides_in_isolated_murine_retroviral_particles","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407528,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407528/thumbnails/1.jpg","file_name":"An_association_between_actin_and_nucleoc20160122-8918-13cdmrm.pdf","download_url":"https://www.academia.edu/attachments/41407528/download_file","bulk_download_file_name":"An_association_between_actin_and_nucleoc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407528/An_association_between_actin_and_nucleoc20160122-8918-13cdmrm-libre.pdf?1453458874=\u0026response-content-disposition=attachment%3B+filename%3DAn_association_between_actin_and_nucleoc.pdf\u0026Expires=1739809200\u0026Signature=FFY4YlvSqXrQnHwT2WB8Qecqhdp24HY8qEeRnrrUjZMIeddeUHmksu5WmzZPP~qm63KQbwIExw9HGBdOuI22qFjp5D2a6NN-UrFZkkOhmiRAPMVZaHSkb6Klx4vey3x2Msa-M~CS2IMq~ha64H1A-W9JBhDTpSZMNQuyD5bDhLQ3aFDoQ7rxIwLYK-hbLtT09uFWMgWmj4xt1xu3SfCmpKARssEQky59Hmy8MEj7n75A~MHR9rAFNvWKvWfr~gVbsvbQ8X4z6Vk4vx39vj5S5wI5jbJ228Pxp3ckkVf~M0PEPfYEKNKgYr~D1Up4K0sIiv-2IXFfMs7CqFAlhRJhfw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503025"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503025/Aggregation_of_membrane_associated_actin_filaments_following_localized_adherence_of_enteropathogenic_Escherichia_coli_to_HeLa_cells"><img alt="Research paper thumbnail of Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells" class="work-thumbnail" src="https://attachments.academia-assets.com/41407543/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503025/Aggregation_of_membrane_associated_actin_filaments_following_localized_adherence_of_enteropathogenic_Escherichia_coli_to_HeLa_cells">Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells</a></div><div class="wp-workCard_item"><span>Journal of cell science</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have previously observed that enteropathogenic Escherichia coli (EPEC) adhere to HeLa cells in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have previously observed that enteropathogenic Escherichia coli (EPEC) adhere to HeLa cells in a localized manner, which we designated localized adherence as opposed to the diffuse pattern of adhesion. In this paper we have examined the effects of localized adherence of EPEC on the actin microfilament system of host HeLa cells. Centrifugation of bacteria onto HeLa cells improved the localized adherence and rapid rearrangements of actin filaments were detected by immunofluorescence and electron microscopy. Aggregation of microfilaments is consistently observed at the sites of localized adherence, and is abolished by cytochalasin D and low temperatures. Scanning electron microscopy indicates that these aggregates are surface microvilli entangled with attached EPEC.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e8021e0d56d4f9b184e81c1882cf8d00" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407543,"asset_id":20503025,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407543/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503025"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503025"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503025; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503025]").text(description); $(".js-view-count[data-work-id=20503025]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503025; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503025']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e8021e0d56d4f9b184e81c1882cf8d00" } } $('.js-work-strip[data-work-id=20503025]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503025,"title":"Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells","internal_url":"https://www.academia.edu/20503025/Aggregation_of_membrane_associated_actin_filaments_following_localized_adherence_of_enteropathogenic_Escherichia_coli_to_HeLa_cells","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407543,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407543/thumbnails/1.jpg","file_name":"Aggregation_of_membrane-associated_actin20160122-17374-oh49me.pdf","download_url":"https://www.academia.edu/attachments/41407543/download_file","bulk_download_file_name":"Aggregation_of_membrane_associated_actin.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407543/Aggregation_of_membrane-associated_actin20160122-17374-oh49me-libre.pdf?1453458875=\u0026response-content-disposition=attachment%3B+filename%3DAggregation_of_membrane_associated_actin.pdf\u0026Expires=1739809200\u0026Signature=ZiyS9sr7zWopoeRmQn~z8JOiND1it51t2L8Hb5Q9bF7NeKzXEcZIF4Iq5yNYCJGWjv-QuUo-lYeTbZw7umomBZQBODniPs7EG7GTUqpuB3GOAyBQPKqcvreFIqtwO8x7NpSnPdvCgR-nGClClrb-eHWYvtIsPWPUdYCK8LwU-bc7T00Qb6nP3oOhqe7aIGE7v2c1rLd0RnP4fXBxUvst9-g7HzCWWsReXfbynpsVEYA44oKmjglAluw5zCm~MfoENRuv0uWvTQsWLAskQxhiCDc178jrwSdvIdo7ozYJDMEjjbyz52WFfK0xFEf-HHotjgLWMC~N76bzATOfU7wpQg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="13368227"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13368227/Martin_BioChem_2010"><img alt="Research paper thumbnail of Martin-BioChem-2010" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/13368227/Martin_BioChem_2010">Martin-BioChem-2010</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ClovisNakaie">Clovis Nakaie</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SShimuta">S. Shimuta</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a></span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13368227"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13368227"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13368227; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13368227]").text(description); $(".js-view-count[data-work-id=13368227]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13368227; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13368227']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13368227]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13368227,"title":"Martin-BioChem-2010","internal_url":"https://www.academia.edu/13368227/Martin_BioChem_2010","owner_id":32599046,"coauthors_can_edit":true,"owner":{"id":32599046,"first_name":"Clovis","middle_initials":null,"last_name":"Nakaie","page_name":"ClovisNakaie","domain_name":"independent","created_at":"2015-06-27T21:23:19.425-07:00","display_name":"Clovis Nakaie","url":"https://independent.academia.edu/ClovisNakaie"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503021"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503021/Expression_inEscherichia_coli_ofa_Dominant_Immunogenof_Trypanosoma_cruzi_Recognized_byHumanChagasic_Sera"><img alt="Research paper thumbnail of Expression inEscherichia coli ofa Dominant Immunogenof Trypanosoma cruzi Recognized byHumanChagasic Sera" class="work-thumbnail" src="https://attachments.academia-assets.com/41407322/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503021/Expression_inEscherichia_coli_ofa_Dominant_Immunogenof_Trypanosoma_cruzi_Recognized_byHumanChagasic_Sera">Expression inEscherichia coli ofa Dominant Immunogenof Trypanosoma cruzi Recognized byHumanChagasic Sera</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A genomic clone expressing a Trypanosoma cruzi antigen in Escherichia coli was identified using h...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A genomic clone expressing a Trypanosoma cruzi antigen in Escherichia coli was identified using human chagasic sera. Chagasic antibodies affinity purified on extracts of this clone recognized a high-molecular-weight protein expressed in all developmental stages of the parasite life cycle, as well as in various T. cruzi strains. The antigen is associated with the cytoskeleton of the parasite and localizes along the attachment region between the flagellum and the cell body. Antibodies to the recombinant antigen were detected in the sera of 115 chagasic patients from different endemic regions, but not in sera of patients with leishmaniasis, T. rangeli infection, or other parasitic diseases. Our data suggest that the presence of antibodies to this antigen may be specifically associated with Chagas' disease.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ffe30e9e11f60e335ede7bb67df68cbb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407322,"asset_id":20503021,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407322/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503021"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503021"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503021; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503021]").text(description); $(".js-view-count[data-work-id=20503021]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503021; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503021']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ffe30e9e11f60e335ede7bb67df68cbb" } } $('.js-work-strip[data-work-id=20503021]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503021,"title":"Expression inEscherichia coli ofa Dominant Immunogenof Trypanosoma cruzi Recognized byHumanChagasic Sera","internal_url":"https://www.academia.edu/20503021/Expression_inEscherichia_coli_ofa_Dominant_Immunogenof_Trypanosoma_cruzi_Recognized_byHumanChagasic_Sera","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407322,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407322/thumbnails/1.jpg","file_name":"Cotrim-1990.pdf","download_url":"https://www.academia.edu/attachments/41407322/download_file","bulk_download_file_name":"Expression_inEscherichia_coli_ofa_Domina.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407322/Cotrim-1990-libre.pdf?1453458889=\u0026response-content-disposition=attachment%3B+filename%3DExpression_inEscherichia_coli_ofa_Domina.pdf\u0026Expires=1739809200\u0026Signature=YizhvZYGjxSp~swwgCc4ZVwgxamMnDBXq-x986wIqaiZm~uaGwpjxDEa6m6RCi~Fex566vHzjWpmRVtagGLD8S0ZTA2ZEyWNnOxURRpSXWRzVVyc-vOQhjCoYolY~pz56qlEBU9SerFX9apWy0Xo73bbvXXD~2W3vnRhYEJV7mOkQeTlQ75HPxbstGVzPrGDBwD9XQZEsf6iJh31lfLkk9ttWHRxjmGBPsBfzwaMd~HeKxGoX6pcqGC3Zv3DN-KYMaKxlgji0lMM8uZDTBzVgan1kjZ-ST1zGKcUWgN4fLacU~PBSTQ04z-WrakZW4E6TTpULGq-3yy6lx30VtoVpQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":41407321,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407321/thumbnails/1.jpg","file_name":"Cotrim-1990.pdf","download_url":"https://www.academia.edu/attachments/41407321/download_file","bulk_download_file_name":"Expression_inEscherichia_coli_ofa_Domina.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407321/Cotrim-1990-libre.pdf?1453458889=\u0026response-content-disposition=attachment%3B+filename%3DExpression_inEscherichia_coli_ofa_Domina.pdf\u0026Expires=1739809200\u0026Signature=NbUkgKKBwoWI9~P2fUOMVBq8zuIoqvAeKlMORFSpngEDrD4btRQULUNJzXPcXjCqzXUoi2yF~je-1HO9gwXIOWGvz3pMSP~F3r8sJv7~gdGWTrniyH7STfwqiRs0xuD8-ZnqSxOhpDpVzCfq-~muf7y2LDg~onRTWsIWxnufg8K3WQ8y-h7Sd6JdsEv7IFjFPcR9SWESRJydCA3IS3j~MErv9oPDjPVquzyTB9-BzuBMQMWLpN83tlvfqNce7L1eY2Umgj7A6gE2~pX8eI1d04saZnaZ7Cm46a1LEpuqkvhVk~ko1Pa7QyO0fNUftfwiNqo8YjJ1IKolWEuo2jcOvQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503018"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503018/Confocal_fluorescence_microscopy_a_powerful_tool_in_the_study_of_Chagas_disease"><img alt="Research paper thumbnail of Confocal fluorescence microscopy: a powerful tool in the study of Chagas' disease" class="work-thumbnail" src="https://attachments.academia-assets.com/41407515/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503018/Confocal_fluorescence_microscopy_a_powerful_tool_in_the_study_of_Chagas_disease">Confocal fluorescence microscopy: a powerful tool in the study of Chagas' disease</a></div><div class="wp-workCard_item"><span>Revista da Sociedade Brasileira de Medicina Tropical</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Confocal scanning fluorescence microscopy has become widely used in cell biology and pathology. I...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Confocal scanning fluorescence microscopy has become widely used in cell biology and pathology. In conjunction with monoclonal antibodies it may turn out to be a powerful diagnostic tool that also enables detailed studies of tissue forms of Trypanosoma cruzi.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="343d7648390a7a0b24924bc185a51410" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407515,"asset_id":20503018,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407515/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503018"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503018"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503018; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503018]").text(description); $(".js-view-count[data-work-id=20503018]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503018; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503018']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "343d7648390a7a0b24924bc185a51410" } } $('.js-work-strip[data-work-id=20503018]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503018,"title":"Confocal fluorescence microscopy: a powerful tool in the study of Chagas' disease","internal_url":"https://www.academia.edu/20503018/Confocal_fluorescence_microscopy_a_powerful_tool_in_the_study_of_Chagas_disease","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407515,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407515/thumbnails/1.jpg","file_name":"Confocal_fluorescence_microscopy_a_power20160122-17380-oh6mnq.pdf","download_url":"https://www.academia.edu/attachments/41407515/download_file","bulk_download_file_name":"Confocal_fluorescence_microscopy_a_power.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407515/Confocal_fluorescence_microscopy_a_power20160122-17380-oh6mnq-libre.pdf?1453458875=\u0026response-content-disposition=attachment%3B+filename%3DConfocal_fluorescence_microscopy_a_power.pdf\u0026Expires=1739809200\u0026Signature=GvIhIUlsO6wB8tnZvX9x4WIftmt4RLVzGnSFMfy-v01RWVi-ETkrUDB8nSABhFrFI47dp~aiWdAlk6xMLwgXdwoanD8HVb5SNyUbSvrL-6In9IlNSK7fEYVGWZwYNIyMNlLN3~GvxQrGDvSrKu~kz-5zEwlCOHHM9vKYrZvgQRpj2r6uSjgqpGNGCCVGjMzZQRvJ9YGWuvx7n74aYajjyYSJ4LyyTbR7B7oiMZphF4nSWCtDYyw6~y~ABcGZj9zSsBpUICjVpnNj0MvHBZwx8ftDc0DLKKVWKB7eAZi~DQ95NXabVkCbAS65kjLhdD8Kq5hPMyWiMPpLB7M9DWIpXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503016"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503016/T_cell_subpopulations_in_myocardial_inflammatory_infiltrates_detected_by_confocal_microscopy_dose_dependence_in_mice_treated_with_cyclophosphamide_during_acute_Trypanosoma_cruziinfection_D%C3%A9tection_de_sous_populations_de_cellules_T_dans_les_infiltrats_inflammatoire_myocardiques_par_microscopie_co_"><img alt="Research paper thumbnail of T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruziinfection Détection de sous-populations de cellules T dans les infiltrats inflammatoire myocardiques par microscopie co..." class="work-thumbnail" src="https://attachments.academia-assets.com/41407324/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503016/T_cell_subpopulations_in_myocardial_inflammatory_infiltrates_detected_by_confocal_microscopy_dose_dependence_in_mice_treated_with_cyclophosphamide_during_acute_Trypanosoma_cruziinfection_D%C3%A9tection_de_sous_populations_de_cellules_T_dans_les_infiltrats_inflammatoire_myocardiques_par_microscopie_co_">T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruziinfection Détection de sous-populations de cellules T dans les infiltrats inflammatoire myocardiques par microscopie co...</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In this article, we have characterized cell subpopulations found in the hearts of mice presenting...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In this article, we have characterized cell subpopulations found in the hearts of mice presenting acute Chagas&#39; disease by immunocy- tochemistry and subjected to different schedules of an immunosuppressive therapy with cyclophosphamide (CY). In this comparative study, CY treatment with different doses was carried out before or after infection with Trypanosoma cruziY strain trypomastigotes, enabling us to discriminate the parasitemic</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="187f062ad78531e3b2279e4db95e8a7e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407324,"asset_id":20503016,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407324/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503016"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503016"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503016; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503016]").text(description); $(".js-view-count[data-work-id=20503016]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503016; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503016']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "187f062ad78531e3b2279e4db95e8a7e" } } $('.js-work-strip[data-work-id=20503016]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503016,"title":"T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruziinfection Détection de sous-populations de cellules T dans les infiltrats inflammatoire myocardiques par microscopie co...","internal_url":"https://www.academia.edu/20503016/T_cell_subpopulations_in_myocardial_inflammatory_infiltrates_detected_by_confocal_microscopy_dose_dependence_in_mice_treated_with_cyclophosphamide_during_acute_Trypanosoma_cruziinfection_D%C3%A9tection_de_sous_populations_de_cellules_T_dans_les_infiltrats_inflammatoire_myocardiques_par_microscopie_co_","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407324,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407324/thumbnails/1.jpg","file_name":"Calabrese-2003a.pdf","download_url":"https://www.academia.edu/attachments/41407324/download_file","bulk_download_file_name":"T_cell_subpopulations_in_myocardial_infl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407324/Calabrese-2003a-libre.pdf?1453458889=\u0026response-content-disposition=attachment%3B+filename%3DT_cell_subpopulations_in_myocardial_infl.pdf\u0026Expires=1739809200\u0026Signature=YaL0xP2pQ4rqjm8zm~Qg2WLP7dNRAMeIYgDo1DSHhb4XGE43uJvIIiCERVW7MqQhZ7E2ofht4oswAC5OfNvgd5CY0wElcrJi73d23FM9elgyXkxtdKIn1oRl-Oj6pgRsehfSLAFL2~TDoTxDHN8gpTkbDTc84YrkQjPpebTOkHtYX52nDYG0XCbAjB07Llux5sXAvKUROezPI6rePXxgry1b-BmSKiMV0DKe7ew3ihWIKUNzCV4A3Rii5AGanjCvkL~MZDeWQEwTmduZwoeljDoK0defUy16zD9jGTWREQuvLdr9u5U8jT7JZGOUPQtOYvg9MkrOcK6afQI9~cKHDQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503013"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503013/Study_of_acute_chagasic_mice_under_immunosuppressive_therapy_by_cyclosporin_A_modulation_and_confocal_analysis_of_inflammatory_reaction"><img alt="Research paper thumbnail of Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction" class="work-thumbnail" src="https://attachments.academia-assets.com/41407500/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503013/Study_of_acute_chagasic_mice_under_immunosuppressive_therapy_by_cyclosporin_A_modulation_and_confocal_analysis_of_inflammatory_reaction">Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction</a></div><div class="wp-workCard_item"><span>Immunopharmacology</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">. The in vivo effects of cyclosporin A CsA on Trypanosoma cruzi infection were examined using dif...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">. The in vivo effects of cyclosporin A CsA on Trypanosoma cruzi infection were examined using different schedules of the drug in mice infected with the Y strain. Parasitaemia at day 8 after infection among CsA-treated animals was usually higher than control infected non-treated mice. On the other hand, mortality analysis showed that animals CsA-treated either Ž . with 200 mgrkg 2 days before infection or with therapeutic doses 10 mgrkg every other day showed almost the same Ž . mean time of death 35.8 and 38.2 days, respectively . In these groups mice died 50% less than control infected non-treated ones. The mean time of death in the animals treated with 200 mgrkg 5 days after infection and in infected non-treated control mice were respectively 29.0 and 22.6 days. The kinetics analysis of the leukocyte population of animals treated with a single dose of 200 mgrkg of CsA before or after infection did not show the alternate pattern of leukopeniarleukocytosis observed in control groups of infected mice but differential cell counts indicated a modulatory action upon circulating leukocytes of therapeutic doses of CsA. The animals treated with any of the CsA schedules showed a moderate to intense diffuse inflammatory reaction exhibiting mainly mononuclear cells in the heart. Immunofluorescence analysis by confocal microscopy revealed that macrophages are a major component of the inflammatory infiltrate in all groups of CsA-treated mice and also in the control group. q</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="151df4891dcc204727223479f607c36e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407500,"asset_id":20503013,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407500/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503013"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503013"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503013; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503013]").text(description); $(".js-view-count[data-work-id=20503013]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503013; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503013']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "151df4891dcc204727223479f607c36e" } } $('.js-work-strip[data-work-id=20503013]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503013,"title":"Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction","internal_url":"https://www.academia.edu/20503013/Study_of_acute_chagasic_mice_under_immunosuppressive_therapy_by_cyclosporin_A_modulation_and_confocal_analysis_of_inflammatory_reaction","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407500,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407500/thumbnails/1.jpg","file_name":"Calabrese-2000.pdf","download_url":"https://www.academia.edu/attachments/41407500/download_file","bulk_download_file_name":"Study_of_acute_chagasic_mice_under_immun.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407500/Calabrese-2000-libre.pdf?1453458878=\u0026response-content-disposition=attachment%3B+filename%3DStudy_of_acute_chagasic_mice_under_immun.pdf\u0026Expires=1739809200\u0026Signature=d5UW3wKAh5vU4oRwLAtf7~SbC5ed-ogMjv38hVa~3BNtUNgYaq3YjFFXOkwFy-CEDrV4tzKfTcmftmzqpp6tUUgkFurwe3ang9r1JuisSqs9n6gAbytcVLfPby7KrOUd7mrX0uFHeVDhEYO9KKqAYN4MCGD6h8NKlsWCR5RNprBMgNXllevN6OR~qXBxfc80hnY~PSQG6KmvTX4iPjANBiVzYlPB1siG3uKusTAsc8K7weXOr0T-nm1j4bg4P8dW5ugAGyjWCK60mAm3v7LaD1C86SIKJi2xSH8cx3Pmyu0GcRbZhb8Zp0AMaAECUlGP6OV~hfhLHO7jcCpt4LnVog__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="1429567" id="papers"><div class="js-work-strip profile--work_container" data-work-id="27504140"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/27504140/Intracellular_localization_of_myeloperoxidase_in_murine_peritoneal_B_lymphocytes_and_macrophages"><img alt="Research paper thumbnail of Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages" class="work-thumbnail" src="https://attachments.academia-assets.com/47760448/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/27504140/Intracellular_localization_of_myeloperoxidase_in_murine_peritoneal_B_lymphocytes_and_macrophages">Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SabrinaOkada">Sabrina Okada</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TomazdeAraujo">Tomaz de H Araújo</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LaryssaFontoura">Laryssa Fontoura</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BAlbuquerque1">B. Albuquerque</a></span></div><div class="wp-workCard_item"><span>Cellular Immunology</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Generation of hypochlorous acid (HOCl), an important microbicidal agent, is considered to be the ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Generation of hypochlorous acid (HOCl), an important microbicidal agent, is considered to be the main function of myeloperoxidase (MPO), an enzyme present in phagocytes. High amounts of MPO are present in neutrophil azurophilic granules, which are mobilized into the phagolysosome vacuole during phagocytosis. MPO is also present in monocytes and macrophages, although to a lesser degree than in neutrophils. In the present study, we investigated the distribution of MPO in murine peritoneal cells using flow cytometry, confocal microscopy (CM) and transmission electron microscopy (TEM). MPO was observed in macrophages, and surprisingly, we detected MPO in B lymphocytes, specifically in B1-a. MPO was present in cytoplasmic granules, vesicles, mitochondria and the nucleus of murine peritoneal cells. Together, these findings suggest that, in addition to its known microbicidal activity, MPO has a myriad of other unanticipated cellular functions.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="befba512c0058510be4585f0dbf5489a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":47760448,"asset_id":27504140,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/47760448/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="27504140"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="27504140"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27504140; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27504140]").text(description); $(".js-view-count[data-work-id=27504140]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27504140; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='27504140']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "befba512c0058510be4585f0dbf5489a" } } $('.js-work-strip[data-work-id=27504140]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":27504140,"title":"Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages","internal_url":"https://www.academia.edu/27504140/Intracellular_localization_of_myeloperoxidase_in_murine_peritoneal_B_lymphocytes_and_macrophages","owner_id":51659245,"coauthors_can_edit":true,"owner":{"id":51659245,"first_name":"Sabrina","middle_initials":null,"last_name":"Okada","page_name":"SabrinaOkada","domain_name":"independent","created_at":"2016-08-03T09:08:44.642-07:00","display_name":"Sabrina Okada","url":"https://independent.academia.edu/SabrinaOkada"},"attachments":[{"id":47760448,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/47760448/thumbnails/1.jpg","file_name":"Intracellular_localization_of_myeloperox20160803-29745-oucixt.pdf","download_url":"https://www.academia.edu/attachments/47760448/download_file","bulk_download_file_name":"Intracellular_localization_of_myeloperox.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/47760448/Intracellular_localization_of_myeloperox20160803-29745-oucixt-libre.pdf?1470241149=\u0026response-content-disposition=attachment%3B+filename%3DIntracellular_localization_of_myeloperox.pdf\u0026Expires=1739809200\u0026Signature=IgssTaD~0aL3xUSJ8dxU2y8G3ssFAJ~EW8xnpRj-x-4pGL4PdSd2T25ZNcxKxwkLmJnrjGVvYav07uYP7TZClL8USDMd~AY68sf2H8lPA96rD869I0QNv0TbJwWl4fbkN9lreB0hvksRcYLdrJwv6fCt1L9Hm6g4Vf~7QjK-wcaliq9wjW-dDlVMX0OeprA0fY4lBgAz74oMG3qM8t~KX7Zg5REZE5qgLDJxiZ-LKejf90BEPL-6FNAjTOq69rU-B7PvexiYUN-HZaOx7Qsq~SBkeesfqCI6biQYWtV0v4DeDKhasOoEy8ZrG-uPHHz3viVuRGBWLe5xZQHLMlbpkQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="27504144"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/27504144/Myeloperoxidase_in_human_peripheral_blood_lymphocytes_Production_and_subcellular_localization"><img alt="Research paper thumbnail of Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/27504144/Myeloperoxidase_in_human_peripheral_blood_lymphocytes_Production_and_subcellular_localization">Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TomazdeAraujo">Tomaz de H Araújo</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SabrinaOkada">Sabrina Okada</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LaryssaFontoura">Laryssa Fontoura</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a></span></div><div class="wp-workCard_item"><span>Cellular immunology</span><span>, Jan 26, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Myeloperoxidase (MPO) is an important enzyme in the front-line protection against microorganisms....</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Myeloperoxidase (MPO) is an important enzyme in the front-line protection against microorganisms. In peripheral blood, it is accepted that MPO is only produced by myeloid-lineage cells. Thus, MPO presence is unexpected in lymphocytes. We showed recently that B1-lymphocytes from mice have MPO. Here, we showed that subsets of human peripheral B, CD4(+) and CD8(+) T lymphocytes express MPO. The content of MPO in lymphocytes was very low compared to neutrophils/monocytes with a preferential distribution in the nucleus and perinuclear region. Also, we performed a MPO mRNA expression analysis from human blood cells derived from microarray raw data publicly available, showing that MPO is modulated in infectious disease. MPO was increased in CD4(+) T lymphocytes from HIV chronic infection and in CD8(+) T lymphocytes from HCV-positive patients. Our study points out MPO as a multifunctional protein due to its subcellular localization and expression modulation in lymphocytes indicating alterna...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="27504144"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="27504144"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27504144; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27504144]").text(description); $(".js-view-count[data-work-id=27504144]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27504144; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='27504144']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=27504144]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":27504144,"title":"Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization","internal_url":"https://www.academia.edu/27504144/Myeloperoxidase_in_human_peripheral_blood_lymphocytes_Production_and_subcellular_localization","owner_id":51659245,"coauthors_can_edit":true,"owner":{"id":51659245,"first_name":"Sabrina","middle_initials":null,"last_name":"Okada","page_name":"SabrinaOkada","domain_name":"independent","created_at":"2016-08-03T09:08:44.642-07:00","display_name":"Sabrina Okada","url":"https://independent.academia.edu/SabrinaOkada"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="12637611"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/12637611/Cell_death_and_regeneration_in_the_midgut_of_the_mosquito_Culex_quinquefasciatus"><img alt="Research paper thumbnail of Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus" class="work-thumbnail" src="https://attachments.academia-assets.com/46034419/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/12637611/Cell_death_and_regeneration_in_the_midgut_of_the_mosquito_Culex_quinquefasciatus">Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/OsvaldoMarinotti">Osvaldo Marinotti</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a></span></div><div class="wp-workCard_item"><span>Journal of Insect Physiology</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Haematophagy, the utilization of blood as food, has evolved independently among insects such as m...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Haematophagy, the utilization of blood as food, has evolved independently among insects such as mosquitoes, bedbugs, fleas, and others. Accordingly, several distinct biological adaptations have occurred in order to facilitate the finding, ingestion and digestion of blood from vertebrate sources. Although blood meals are essential for survival and reproduction of these insects, mechanical and chemical stresses are caused by the ingestion of a sizable meal (frequently twice or more times the weight of the insect) containing large amounts of cytotoxic molecules such as haem. Here we present data showing that the stresses caused by a blood meal induce cell death in the midgut epithelium of Culex quinquefasciatus mosquitoes. The process involves apoptosis, ejection of dead cells to the midgut lumen and differentiation of basal regenerative cells to replace the lost digestive cells. The basal cell differentiation in blood-fed mosquito midguts represents an additional mechanism by which insects cope with the stresses caused by blood meals. C. quinquefasciatus adult females are unable to replace lost cells following a third or fourth blood meal, which may have a significant impact on mosquito longevity, reproduction and vectorial capacity. r</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a9f23997608ec7b409ba266f9127be74" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":46034419,"asset_id":12637611,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/46034419/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="12637611"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="12637611"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 12637611; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=12637611]").text(description); $(".js-view-count[data-work-id=12637611]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 12637611; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='12637611']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a9f23997608ec7b409ba266f9127be74" } } $('.js-work-strip[data-work-id=12637611]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":12637611,"title":"Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus","internal_url":"https://www.academia.edu/12637611/Cell_death_and_regeneration_in_the_midgut_of_the_mosquito_Culex_quinquefasciatus","owner_id":31609855,"coauthors_can_edit":true,"owner":{"id":31609855,"first_name":"Osvaldo","middle_initials":null,"last_name":"Marinotti","page_name":"OsvaldoMarinotti","domain_name":"independent","created_at":"2015-05-27T14:50:57.290-07:00","display_name":"Osvaldo Marinotti","url":"https://independent.academia.edu/OsvaldoMarinotti"},"attachments":[{"id":46034419,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/46034419/thumbnails/1.jpg","file_name":"Cell_death_And_regeneration_in_the_midgu20160528-14996-vakybp.pdf","download_url":"https://www.academia.edu/attachments/46034419/download_file","bulk_download_file_name":"Cell_death_and_regeneration_in_the_midgu.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/46034419/Cell_death_And_regeneration_in_the_midgu20160528-14996-vakybp-libre.pdf?1464503605=\u0026response-content-disposition=attachment%3B+filename%3DCell_death_and_regeneration_in_the_midgu.pdf\u0026Expires=1739809200\u0026Signature=GAnnDVBstvfgtOKSNYdLhABDrGOIo8IedZ~cF~8qSE3w8uXlwlDlQDzjP9AZkZiWox72-CWEtHB0MxK4FYtiCiz-khava2A8c4j11or1Fopth5w03FlAwu7ZPc9ozc6rPnfo~WQNgQWq9~YTzeArBtsJ3iccEcIE02tm1grjYG3-SmefPvQucrT68iCCmtYwJpZtyKxgzqyxTp0R85uSR1NFyqrPemNYh7yzPLASSqyk4J~Bt~QwSOIMy1DOeMJ5kuJh1qhhCchfodFYiceFEBLjhLwQ1Q3Vi8vBjbRlszICm5~tWAwITTqgQD794DM6SbSn-SHBjU8n4Yg1WSO1MA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="24946260"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/24946260/Trypanosoma_cruzi_single_cell_live_imaging_inside_infected_tissues"><img alt="Research paper thumbnail of Trypanosoma cruzi: single cell live imaging inside infected tissues" class="work-thumbnail" src="https://attachments.academia-assets.com/45277126/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/24946260/Trypanosoma_cruzi_single_cell_live_imaging_inside_infected_tissues">Trypanosoma cruzi: single cell live imaging inside infected tissues</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/EstebanCordero1">Esteban Cordero</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RMortara">Renato Mortara</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Although imaging the live Trypanosoma cruzi parasite is a routine technique in most laboratories,...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Although imaging the live Trypanosoma cruzi parasite is a routine technique in most laboratories, identification of the parasite in infected tissues and organs has been hindered by their intrinsic opaque nature. We describe a simple method for in vivo observation of live single cell Trypanosoma cruzi parasites inside mammalian host tissues. BALB/c or C57BL/6 mice infected with DsRed-CL or GFP-G trypomastigotes had their organs removed and sectioned with surgical blades. Ex vivo organ sections were observed under confocal microscopy. For the first time, this procedure enabled imaging of individual amastigotes, intermediate forms and motile trypomastigotes within infected tissues of mammalian hosts.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="34a420d7161e34a4648e6e36d4734f4a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45277126,"asset_id":24946260,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45277126/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24946260"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24946260"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24946260; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24946260]").text(description); $(".js-view-count[data-work-id=24946260]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24946260; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='24946260']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "34a420d7161e34a4648e6e36d4734f4a" } } $('.js-work-strip[data-work-id=24946260]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":24946260,"title":"Trypanosoma cruzi: single cell live imaging inside infected tissues","internal_url":"https://www.academia.edu/24946260/Trypanosoma_cruzi_single_cell_live_imaging_inside_infected_tissues","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":45277126,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45277126/thumbnails/1.jpg","file_name":"Ferreira_Cell_Microbiology.pdf","download_url":"https://www.academia.edu/attachments/45277126/download_file","bulk_download_file_name":"Trypanosoma_cruzi_single_cell_live_imagi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45277126/Ferreira_Cell_Microbiology-libre.pdf?1462197345=\u0026response-content-disposition=attachment%3B+filename%3DTrypanosoma_cruzi_single_cell_live_imagi.pdf\u0026Expires=1739809200\u0026Signature=KGU~8mh048HMp2rhizv0bF1j4gwdUqPo53M4B03VhrmtkjOyAku0SQJELll2F7I8WIHmleXkpCm36imugQIMtIuNpMs0USmmCdv--z4qSSBj6cd-3U-yJcsKpm8D5BE7cKYl5HxdnvpaDlycRfcFDMP5M5rKK2XNA6ekBTL1JI59RxrPjXyDdTYJLxnqLk2a~TwGBTdG-dNCg~P9NolL25fXso4dNuvrwY3~oeKgK1WJ53eoRtmHUaTnQtUQbQwMomlSoE-c~wfhXQ3jmVxVWrwxMYccI23aeivtz3ej74fkrFoG-udCz8gOReu6MxqOlsgNokXUEwqhSv~CnkggMA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="23663028"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/23663028/Ultrastructural_and_cytochemical_identification_of_megasome_in_Leishmania_Leishmania_chagasi"><img alt="Research paper thumbnail of Ultrastructural and cytochemical identification of megasome in Leishmania (Leishmania) chagasi" class="work-thumbnail" src="https://attachments.academia-assets.com/44069638/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/23663028/Ultrastructural_and_cytochemical_identification_of_megasome_in_Leishmania_Leishmania_chagasi">Ultrastructural and cytochemical identification of megasome in Leishmania (Leishmania) chagasi</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uepa.academia.edu/SannyAlberio">Sanny Alberio</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a></span></div><div class="wp-workCard_item"><span>Parasitology Research</span><span>, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The present work showed the presence of a megasome in Leishmania (Leishmania) chagasi amastigotes...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The present work showed the presence of a megasome in Leishmania (Leishmania) chagasi amastigotes. Transmission electron microscopy analysis of ultrathin serial sections and three-dimensional reconstruction allowed visualization of large structures in amastigote forms of L. (L.) chagasi and a multivesicular tubule-lysosome structure in metacyclic promastigotes. Morphometric data showed that the relative volume occupied by the megasome and the multivesicular tubule (MVT)-lysosome structures was about 5% and 3.2%, respectively, in amastigotes and promastigotes of L. (L.) chagasi. Further characterization of the megasome in L. (L.) chagasi amastigotes was carried out by immunolabeling of cysteine proteinase, whereas the lysosomal content of amastigotes and promastigotes was confirmed by arylsulfatase cytochemistry.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cf6d4fca172c1e0102da7851a11f69fa" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44069638,"asset_id":23663028,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44069638/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="23663028"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="23663028"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23663028; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23663028]").text(description); $(".js-view-count[data-work-id=23663028]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23663028; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='23663028']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cf6d4fca172c1e0102da7851a11f69fa" } } $('.js-work-strip[data-work-id=23663028]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":23663028,"title":"Ultrastructural and cytochemical identification of megasome in Leishmania (Leishmania) chagasi","internal_url":"https://www.academia.edu/23663028/Ultrastructural_and_cytochemical_identification_of_megasome_in_Leishmania_Leishmania_chagasi","owner_id":45723431,"coauthors_can_edit":true,"owner":{"id":45723431,"first_name":"Sanny","middle_initials":null,"last_name":"Alberio","page_name":"SannyAlberio","domain_name":"uepa","created_at":"2016-03-24T07:49:40.951-07:00","display_name":"Sanny Alberio","url":"https://uepa.academia.edu/SannyAlberio"},"attachments":[{"id":44069638,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/44069638/thumbnails/1.jpg","file_name":"Alberio-2004.pdf","download_url":"https://www.academia.edu/attachments/44069638/download_file","bulk_download_file_name":"Ultrastructural_and_cytochemical_identif.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/44069638/Alberio-2004-libre.pdf?1458831393=\u0026response-content-disposition=attachment%3B+filename%3DUltrastructural_and_cytochemical_identif.pdf\u0026Expires=1739809200\u0026Signature=gD3FMHgJucg1eeJH4R1lPlAd8ef4gXlToAwYdRRuSf5HZ5Fju0b~FrdHi6sxuFz9rOfKPOAc4-xbP3PTxw9Z2qCQA6~GEtr32HBCkTABKUmaWVcKEE-g44STAmkjopYvORQole~ZCR4-nVB5hKi1cyRYq81970SqKVOL0ZP3SfjOopJZV7Us7Vbsn~rih~NgY6jMiAaUfIaTlk0~AWoNlimD9MaThRQYghVMizQqsb~QoTYmD9hHY352M0nFRMFGsZsuWP79vU0D79KXo0y6JCmQQ5-Zzjfh6G4ERXFBj9yqAXU56zEEMyi6OfrSEP-SI9lNl11oPU1yf6lt5L81xg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503044"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503044/Revealing_Annexin_A2_and_ARF_6_enrollment_during_Trypanosoma_cruzi_extracellular_amastigote_host_cell_interaction"><img alt="Research paper thumbnail of Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction" class="work-thumbnail" src="https://attachments.academia-assets.com/41407527/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503044/Revealing_Annexin_A2_and_ARF_6_enrollment_during_Trypanosoma_cruzi_extracellular_amastigote_host_cell_interaction">Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction</a></div><div class="wp-workCard_item"><span>Parasites & vectors</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerizatio...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Invasion of host cells by Trypanosoma cruzi extracellular amastigotes is host actin polymerization-dependent. However, the role of proteins related to actin dynamics during invasion by amastigotes remains to be investigated. Here we describe the role of Annexin A2 and ARF-6 during extracellular amastigote-mammalian cell interactions. Our results showed ARF-6 accumulation in the amastigote-containing parasitophorous vacuole containing amastigote forms; demonstrated ARF-6 and Annexin A2 critical impact over parasite cell invasion and revealed the effect of Annexin A2 expression on intracellular parasite multiplication. ARF-6 and Annexin A2 are involved in invasion of mammalian cells by T. cruzi amastigotes.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="77c916570ef0a4a87175514f76329645" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407527,"asset_id":20503044,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407527/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503044"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503044"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503044; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503044]").text(description); $(".js-view-count[data-work-id=20503044]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503044; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503044']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "77c916570ef0a4a87175514f76329645" } } $('.js-work-strip[data-work-id=20503044]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503044,"title":"Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction","internal_url":"https://www.academia.edu/20503044/Revealing_Annexin_A2_and_ARF_6_enrollment_during_Trypanosoma_cruzi_extracellular_amastigote_host_cell_interaction","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407527,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407527/thumbnails/1.jpg","file_name":"563cee0308ae45b5d289962a.pdf","download_url":"https://www.academia.edu/attachments/41407527/download_file","bulk_download_file_name":"Revealing_Annexin_A2_and_ARF_6_enrollmen.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407527/563cee0308ae45b5d289962a-libre.pdf?1453458874=\u0026response-content-disposition=attachment%3B+filename%3DRevealing_Annexin_A2_and_ARF_6_enrollmen.pdf\u0026Expires=1739809200\u0026Signature=P7xSSMFQ1D79cL-cwoPA4Nr7125R8Awz3~yalTqHbUgm9skriov-aZsLCcxlRttka-tRKq9qBU9M0HhtoTcMLC34DyFUbBhTPYk22IoDLVqOm-7PfX6k7rGFMQFcix8rAffCn6BC-tXR6kZF23PTFprP~JrOTfEy6TC~YSjffdfmLTdZ2KqdH69E~vBjHXAwhf9CN95IHO8-uzXU-sshgCdXP9OZwgu7fxmA7gYx9RSL4bHIyBu95bexQ7lnXYDYderbOmJP1RIgtFPTCu-lqGQbVQNsu-bgTc-O-f5BnTxZeNWzoU2vLOr7liky9pKZgAxIfnjLqHeVyeIYWn96bw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503042"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/20503042/Recruitment_of_PH_AKT_to_nascent_phagosomes_containing_T_cruzi_G_strain_EAs"><img alt="Research paper thumbnail of Recruitment of PH-AKT to nascent phagosomes containing T. cruzi G strain EAs" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/20503042/Recruitment_of_PH_AKT_to_nascent_phagosomes_containing_T_cruzi_G_strain_EAs">Recruitment of PH-AKT to nascent phagosomes containing T. cruzi G strain EAs</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503042"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503042"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503042; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503042]").text(description); $(".js-view-count[data-work-id=20503042]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503042; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503042']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=20503042]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503042,"title":"Recruitment of PH-AKT to nascent phagosomes containing T. cruzi G strain EAs","internal_url":"https://www.academia.edu/20503042/Recruitment_of_PH_AKT_to_nascent_phagosomes_containing_T_cruzi_G_strain_EAs","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503041"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503041/Phosphatidylinositol_Specific_Phospholipase_C_PI_PLC_Cleavage_of_GPI_Anchored_Surface_Molecules_of_Trypanosoma_cruzi_Triggers_In_Vitro_Morphological_Reorganization_of_Trypomastigotes"><img alt="Research paper thumbnail of Phosphatidylinositol‐Specific Phospholipase C (PI‐PLC) Cleavage of GPI‐Anchored Surface Molecules of Trypanosoma cruzi Triggers In Vitro Morphological Reorganization of Trypomastigotes" class="work-thumbnail" src="https://attachments.academia-assets.com/41407688/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503041/Phosphatidylinositol_Specific_Phospholipase_C_PI_PLC_Cleavage_of_GPI_Anchored_Surface_Molecules_of_Trypanosoma_cruzi_Triggers_In_Vitro_Morphological_Reorganization_of_Trypomastigotes">Phosphatidylinositol‐Specific Phospholipase C (PI‐PLC) Cleavage of GPI‐Anchored Surface Molecules of Trypanosoma cruzi Triggers In Vitro Morphological Reorganization of Trypomastigotes</a></div><div class="wp-workCard_item"><span>Journal of Eukaryotic Microbiology</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Trypanosoma cruzi trypomastigotes treated with phosphatidylinositol-specific phospholipase C (PI-...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Trypanosoma cruzi trypomastigotes treated with phosphatidylinositol-specific phospholipase C (PI-PLC) in vitro are rapidly induced to differentiate into round forms. Using confocal microscopy, we were able to show that trypomastigotes treated with PI-PLC initiate the process of flagellum remodeling by 30 sec after contact with the enzyme and amastigote-like forms are detected as early as 10 min after PI-PLC treatment. Scanning and transmission electron microscopy indicate that trypomastigotes undergo a previously undescribed process of flagellum circularization and internalization. Analysis of the flagellar complex with monoclonal antibody 4D9 shows heterogeneous labeling among the parasites, suggesting a remodeling of these molecules. After PI-PLC treatment, parasites rapidly lose the surface marker Ssp-3 and 24 h post-treatment they begin to exhibit a circular nucleus and a rod-shaped kinetoplast. By flow cytometry analysis and confocal microscopy, the Ssp-4 amastigote-specific ep...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dec4adb7c9e77623c27d1e954648a710" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407688,"asset_id":20503041,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407688/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503041"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503041"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503041; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503041]").text(description); $(".js-view-count[data-work-id=20503041]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503041; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503041']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "dec4adb7c9e77623c27d1e954648a710" } } $('.js-work-strip[data-work-id=20503041]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503041,"title":"Phosphatidylinositol‐Specific Phospholipase C (PI‐PLC) Cleavage of GPI‐Anchored Surface Molecules of Trypanosoma cruzi Triggers In Vitro Morphological Reorganization of Trypomastigotes","internal_url":"https://www.academia.edu/20503041/Phosphatidylinositol_Specific_Phospholipase_C_PI_PLC_Cleavage_of_GPI_Anchored_Surface_Molecules_of_Trypanosoma_cruzi_Triggers_In_Vitro_Morphological_Reorganization_of_Trypomastigotes","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407688,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407688/thumbnails/1.jpg","file_name":"PhosphatidylinositolSpecific_Phospholipa20160122-30106-63ircd.pdf","download_url":"https://www.academia.edu/attachments/41407688/download_file","bulk_download_file_name":"Phosphatidylinositol_Specific_Phospholip.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407688/PhosphatidylinositolSpecific_Phospholipa20160122-30106-63ircd-libre.pdf?1453458908=\u0026response-content-disposition=attachment%3B+filename%3DPhosphatidylinositol_Specific_Phospholip.pdf\u0026Expires=1739809200\u0026Signature=YcPxb-LyTjkW8XBHd8N6r67GUsWESu9uDe~Tnk5jGYvT~3YBHFLS5pFMqqV3OqlHkt5gUicTStjNsdE7BeOEHuY75DQ-hThYzKfC~kJaWVrj03O79jMWILSRb~UC-PJNh~nVwzBJXxkoUwxggHCCU8BESer-oSwR1iD5TA7V7gkm~a9eLkdbATuKPQIsccO6ZFIq~Z8dTH4owHvhe7b8Jksi8H6InDkXocK7AcgfSY2L5-53A3V~U6Pkn63RAID5OVLm48~Gy7IyZ07t3rk~rU3VNw-rx-C3i8-uOnArT6zgkE4g8Oe0fp-bDvh4ao70CTsrSHqtkiOm2id2lsiIeA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503039"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503039/Detection_and_localization_of_small_nuclear_ribonucleoproteins_snRNPs_in_trypanosomatids_using_anti_m3G_antibodies"><img alt="Research paper thumbnail of Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies" class="work-thumbnail" src="https://attachments.academia-assets.com/41407526/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503039/Detection_and_localization_of_small_nuclear_ribonucleoproteins_snRNPs_in_trypanosomatids_using_anti_m3G_antibodies">Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies</a></div><div class="wp-workCard_item"><span>Japanese journal of infectious diseases</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This work reports for the first time the identification and immunolocalization, by confocal and c...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This work reports for the first time the identification and immunolocalization, by confocal and conventional indirect immunofluorescence, of m3G epitopes present in ribonucleoproteins of the following trypanosomatids: Trypanosoma cruzi epimastigotes of three different strains, Blastocrithidia ssp., and Leishmania major promastigotes. The identity of these epitopes and hence the specificity of the anti-m3G monoclonal antibody were ascertained through competition reaction with 7-methylguanosine that blocks the Ig binding sites, abolishing the fluorescence in all the parasites tested and showing a specific perinuclear localization of the snRNPs, which suggests their nuclear reimport in the parasites. Using an immunoprecipitation technique, it was also possible to confirm the presence of the trimethylguanosine epitopes in trypanosomatids.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9a4254e72c45becf13d650b4a98605e5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407526,"asset_id":20503039,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407526/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503039"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503039"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503039; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503039]").text(description); $(".js-view-count[data-work-id=20503039]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503039; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503039']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9a4254e72c45becf13d650b4a98605e5" } } $('.js-work-strip[data-work-id=20503039]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503039,"title":"Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies","internal_url":"https://www.academia.edu/20503039/Detection_and_localization_of_small_nuclear_ribonucleoproteins_snRNPs_in_trypanosomatids_using_anti_m3G_antibodies","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407526,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407526/thumbnails/1.jpg","file_name":"Bosetto-2008.pdf","download_url":"https://www.academia.edu/attachments/41407526/download_file","bulk_download_file_name":"Detection_and_localization_of_small_nucl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407526/Bosetto-2008-libre.pdf?1453458874=\u0026response-content-disposition=attachment%3B+filename%3DDetection_and_localization_of_small_nucl.pdf\u0026Expires=1739809200\u0026Signature=fwz3dO0~uivZ219GDgag57pXNFK0b-Tg5rNnz68xWMaKcIIPc7u~KbcnAC3vk2rw71-VFRblIfhSdvo4J0dvaO1wMnYVvJzbZkUrpFGirkRAFo5cxtclyZn30KJlCNwqt2CM7YXNwzdk3FWNN3WYNgenam0XvMpK5ho~vjVfNPGFC8Y3hq8IkiVBkZqY4VF~MTgeUxWfLrc1Tk9EhF8SBo1AyMrgnJI6ItX0VhHChBgu9aWQIxjt8G~DztF8bc1zVTXTIZ8qqhe9zeegMHaOeGs7a12YOMycKS2C-K070bUrrIrcSXI73lJ~oWVLLmcLXPP~Qj3seIKRejtwYagvlw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503036"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503036/Polymorphism_ofthe35_and50_Kilodalton_Surface_Glycoconjugates_ofTrypanosoma_cnrzi_Metacyclic_Trypomastigotes"><img alt="Research paper thumbnail of Polymorphism ofthe35-and50-Kilodalton Surface Glycoconjugates ofTrypanosoma cnrzi Metacyclic Trypomastigotes" class="work-thumbnail" src="https://attachments.academia-assets.com/41407689/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503036/Polymorphism_ofthe35_and50_Kilodalton_Surface_Glycoconjugates_ofTrypanosoma_cnrzi_Metacyclic_Trypomastigotes">Polymorphism ofthe35-and50-Kilodalton Surface Glycoconjugates ofTrypanosoma cnrzi Metacyclic Trypomastigotes</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In this study, we examined the immunochemical properties of the 35and 50-kDa (35/50-kDa) surface ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In this study, we examined the immunochemical properties of the 35and 50-kDa (35/50-kDa) surface glycoconjugates expressed on the surface of metacyclic trypomastigotes of Trypanosoma cruzi using three different monoclonal antibodies directed to this component. The 35/50-kDa surface antigen was expressed by metacycic trypomastigotes of 11 different strains of T. cruzi and displayed a significant degree of molecular polymorphism. Results of immunoblotting and complement-mediated lysis were consistent with such diversity. Different monoclonal antibodies reacted with distinct epitopes on the 35/50-kDa antigen, which is resistant to proteases and behaves as an amphiphilic component.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0c519c62169fef7c9a4df7c601310767" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407689,"asset_id":20503036,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407689/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503036"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503036"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503036; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503036]").text(description); $(".js-view-count[data-work-id=20503036]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503036; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503036']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0c519c62169fef7c9a4df7c601310767" } } $('.js-work-strip[data-work-id=20503036]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503036,"title":"Polymorphism ofthe35-and50-Kilodalton Surface Glycoconjugates ofTrypanosoma cnrzi Metacyclic Trypomastigotes","internal_url":"https://www.academia.edu/20503036/Polymorphism_ofthe35_and50_Kilodalton_Surface_Glycoconjugates_ofTrypanosoma_cnrzi_Metacyclic_Trypomastigotes","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407689,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407689/thumbnails/1.jpg","file_name":"Polymorphism_ofthe35-and50-Kilodalton_Su20160122-17380-1ryipy0.pdf","download_url":"https://www.academia.edu/attachments/41407689/download_file","bulk_download_file_name":"Polymorphism_ofthe35_and50_Kilodalton_Su.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407689/Polymorphism_ofthe35-and50-Kilodalton_Su20160122-17380-1ryipy0-libre.pdf?1453458909=\u0026response-content-disposition=attachment%3B+filename%3DPolymorphism_ofthe35_and50_Kilodalton_Su.pdf\u0026Expires=1739809200\u0026Signature=SgVqm02hftxO0dyHCKlG~1WNe69NtBUreqAzt5t-Gp~qt5FRt0zLVdbnlLjrrUIg5SphN9LzkEUvV-LhEn-0q1HwoPQE20R~4T744hDghuVVS3ZBwQNqWVoi8Tat0kXSJZawy6~-O~r35N8Jz6MXvqyrWTLdZoAAH0ZRTcHT1F5r-MDojjHPN9KgZvIQ~1QDbdHvXQawspjshe-8kAPh3lF9iPGyu41ywmhnXu1PZvksj4BuxRSUfynuiyTjU-lTmPw4zB3mN933KKcP3cRBn6Is84fqc4ba43OEmDDTlcttCUyxpPagNQjL1ohvYbtk1IcguLB9bdHwt0-LIdfSLA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503034"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503034/Central_nervous_system_involvement_in_experimental_infection_with_Leishmania_Leishmania_amazonensis"><img alt="Research paper thumbnail of Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis" class="work-thumbnail" src="https://attachments.academia-assets.com/41407516/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503034/Central_nervous_system_involvement_in_experimental_infection_with_Leishmania_Leishmania_amazonensis">Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis</a></div><div class="wp-workCard_item"><span>The American journal of tropical medicine and hygiene</span><span>, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We describe the pathologic alterations of the central nervous system (CNS) observed in experiment...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We describe the pathologic alterations of the central nervous system (CNS) observed in experimental tegumentary leishmaniasis in BALB/c and Swiss mice. The mice were subcutaneously infected with 10(4) amastigotes of Leishmania (Leishmania) amazonensis. Animals were killed and brains were removed for histologic and immunocytochemical studies. Histologic examination showed that 66.6% of infected mice had a discrete hyperemia and inflammatory infiltrate in the meninges, composed of mononuclear cells and neutrophils with no detectable parasites. However, parasitized macrophages were detected in the cerebral parenchyma, as well as mast cells, lymphocytes, and polymorphonuclear cells. Necrosis in the cerebral parenchyma was also observed. Confocal fluorescence microscopy showed that CD8+ T lymphocytes are the major component of the inflammatory infiltrate in the CNS. In addition to these cells, CD4+, CD11b, and dendritic cells are present, in small numbers, in the inflammatory processes o...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7ef5b83a552453af2a5137f00f8d44db" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407516,"asset_id":20503034,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407516/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503034"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503034"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503034; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503034]").text(description); $(".js-view-count[data-work-id=20503034]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503034; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503034']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7ef5b83a552453af2a5137f00f8d44db" } } $('.js-work-strip[data-work-id=20503034]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503034,"title":"Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis","internal_url":"https://www.academia.edu/20503034/Central_nervous_system_involvement_in_experimental_infection_with_Leishmania_Leishmania_amazonensis","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407516,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407516/thumbnails/1.jpg","file_name":"661.pdf","download_url":"https://www.academia.edu/attachments/41407516/download_file","bulk_download_file_name":"Central_nervous_system_involvement_in_ex.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407516/661-libre.pdf?1453458875=\u0026response-content-disposition=attachment%3B+filename%3DCentral_nervous_system_involvement_in_ex.pdf\u0026Expires=1739809200\u0026Signature=DlHrr1FsyfjLTWNy9iT5dnKUmMqVWfhtQbQz-ajLRFnSg60W0DZm2-tAUBUbeG8fZ3jUx1OKyw1UEa0aJbP3wxSxiistN-FIBETHUw9MkObsuH1xQOZLhzdQU9~BwJm1xbiVsa7f839X4NRDwKn3E-LJW0LSTZJL-ukKZOkVynjRiO8TqaYNb4wFKD-qyr61FlICj-3QcxtP6He10E6vYhc4YUszPkX9F~-cR-rgsLHSU3CE8OyVtnq42JRu-eF6Jnn~8JH~~w5lHTaW-eHKKh2XaK~nK1w9O2EvqUZHevwFt4owptnOpQ3efsF3w1sC9UuzWauQ~kl-Esy2nfhNNg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503032"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503032/Electrophoretic_karyotypes_and_genome_sizing_of_the_pathogenic_fungus_Paracoccidioides_brasiliensis"><img alt="Research paper thumbnail of Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis" class="work-thumbnail" src="https://attachments.academia-assets.com/41407513/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503032/Electrophoretic_karyotypes_and_genome_sizing_of_the_pathogenic_fungus_Paracoccidioides_brasiliensis">Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis</a></div><div class="wp-workCard_item"><span>Journal of clinical microbiology</span><span>, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Here we present the karyotype analysis and genome sizing of Paracoccidioides brasiliensis, a path...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Here we present the karyotype analysis and genome sizing of Paracoccidioides brasiliensis, a pathogen refractory to conventional genetic analysis. We have established pulsed-field gel electrophoresis (PFGE) conditions to resolve the high-molecular-weight chromosomal bands of two clinical isolates of P. brasiliensis. Both isolates showed four megabase-sized bands, ranging from 2.0 to 10.0 Mbp. Significant differences in chromosome sizes and in the chromosomal location of genes for the gp43 antigen and chitin synthase were found. Different technical approaches were employed to estimate the DNA content and to define the ploidy of P. brasiliensis. An estimated genome size in the range of 45.7 to 60.9 Mbp was provided by the analysis of data generated by measuring the amplitude of fluorescence intensity of DAPI (4&#39;,6-diamidino-2-phenylindole)-stained nuclei (by confocal microscopy). The nuclear genome size estimated by confocal microscopy is twice that estimated by the average sum of...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="90a5e177bea4b6fabae5ea26661e6fc0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407513,"asset_id":20503032,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407513/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503032"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503032"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503032; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503032]").text(description); $(".js-view-count[data-work-id=20503032]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503032; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503032']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "90a5e177bea4b6fabae5ea26661e6fc0" } } $('.js-work-strip[data-work-id=20503032]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503032,"title":"Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis","internal_url":"https://www.academia.edu/20503032/Electrophoretic_karyotypes_and_genome_sizing_of_the_pathogenic_fungus_Paracoccidioides_brasiliensis","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407513,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407513/thumbnails/1.jpg","file_name":"Cano-1998.pdf","download_url":"https://www.academia.edu/attachments/41407513/download_file","bulk_download_file_name":"Electrophoretic_karyotypes_and_genome_si.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407513/Cano-1998-libre.pdf?1453458875=\u0026response-content-disposition=attachment%3B+filename%3DElectrophoretic_karyotypes_and_genome_si.pdf\u0026Expires=1739809200\u0026Signature=J4X2-kiNEy4Ffb2HaX2AFjwBkWkFVDWslThDNvgghgpytfli3bQuzcaHqDbZI~8m9WUedc3VZDySnsXySGb5-X5evW8jS0gPuWAY9-mIc46ApgW-4v2klj74S~RkNwQnFwtUjX4EbyFiJj0Cob4Q0NXboIiMEphhY0HzGM9T0SW7qX-ce8Wu4TAqpvA7Btz9AtS5t0gZGyw0js0HrJmkiZSWmj8rb3QSp~PtmdXixVIe3vdVc6GPQu8vhA8x4UsT1-OlmGfcmPsXA0SQPcerKwuG-bPdDuZi4usESovnDYBzFlfybSPXScNavDbLpw0pCzavpDPoiyNeYk4D-ElSVQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503030"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503030/Endoplasmic_reticulum_contains_a_common_abundant_calcium_binding_glycoprotein_endoplasmin"><img alt="Research paper thumbnail of Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin" class="work-thumbnail" src="https://attachments.academia-assets.com/41407530/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503030/Endoplasmic_reticulum_contains_a_common_abundant_calcium_binding_glycoprotein_endoplasmin">Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin</a></div><div class="wp-workCard_item"><span>Journal of cell science</span><span>, 1986</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The most abundant protein in microsomal membrane preparations from mammalian cells has been ident...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The most abundant protein in microsomal membrane preparations from mammalian cells has been identified as a 100 X 10(3) Mr concanavalin A-binding glycoprotein. The glycosyl moiety of the glycoprotein is completely sensitive to endoglycosidase H, suggesting a predominantly endoplasmic reticulum localization in the cell. Using a monospecific antibody it was shown by binding and immunofluorescence studies that the glycoprotein is intracellular. Immunoelectron microscopy showed that the glycoprotein was at least 100 times more concentrated in the endoplasmic reticulum than in any other cellular organelle. It was found to be substantially overexpressed in cells and tissues rich in endoplasmic reticulum. Since it is the major common protein component associated with the endoplasmic reticulum we refer to it as endoplasmin. Calcium-binding studies show that endoplasmin is a major calcium-binding protein in cells, suggesting that at least one of its roles might be in the calcium-storage func...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fff614aae6ec6a0e49e0c41045dc4bb0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407530,"asset_id":20503030,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407530/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503030"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503030"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503030; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503030]").text(description); $(".js-view-count[data-work-id=20503030]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503030; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503030']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fff614aae6ec6a0e49e0c41045dc4bb0" } } $('.js-work-strip[data-work-id=20503030]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503030,"title":"Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin","internal_url":"https://www.academia.edu/20503030/Endoplasmic_reticulum_contains_a_common_abundant_calcium_binding_glycoprotein_endoplasmin","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407530,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407530/thumbnails/1.jpg","file_name":"Endoplasmic_reticulum_contains_a_common_20160122-6217-14ilvzh.pdf","download_url":"https://www.academia.edu/attachments/41407530/download_file","bulk_download_file_name":"Endoplasmic_reticulum_contains_a_common.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407530/Endoplasmic_reticulum_contains_a_common_20160122-6217-14ilvzh-libre.pdf?1453458877=\u0026response-content-disposition=attachment%3B+filename%3DEndoplasmic_reticulum_contains_a_common.pdf\u0026Expires=1739809200\u0026Signature=Ma2Ba1KV0gYYnspD8Pmzj5KVaVp1QZT8Wd3kBm3CbRTpnlz2HH-BxhPVNqcf7ibcJvtSrn3eWS0eEyeUH2e71shFeV6G19pVobf9ij1laKHr3UsaT3lMN-ugfBR-nR2TfCaqafTr~wUaxL8zhyKb1x8D2u5GF-ef7V52euMQoZ2-zuYcirSr3L2Fk8D6qNuQ12iuxx8q29IK9~JKIt5auwvVNOidFFJxRuQwNDsUqHbLcyoyh6AHvfYIds2jQoanG3fMy96obxzhn8u41YRwS4kSGTjXIMhTljvDXcHI9sNdzkoHISkXtp0lR1utBtn7s5tIwd65D4OIa7PHJ-vqxg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503027"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503027/An_association_between_actin_and_nucleocapsid_polypeptides_in_isolated_murine_retroviral_particles"><img alt="Research paper thumbnail of An association between actin and nucleocapsid polypeptides in isolated murine retroviral particles" class="work-thumbnail" src="https://attachments.academia-assets.com/41407528/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503027/An_association_between_actin_and_nucleocapsid_polypeptides_in_isolated_murine_retroviral_particles">An association between actin and nucleocapsid polypeptides in isolated murine retroviral particles</a></div><div class="wp-workCard_item"><span>Journal of submicroscopic cytology and pathology</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mammalian cells infected with retroviruses frequently display virus particles budding at the tip ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mammalian cells infected with retroviruses frequently display virus particles budding at the tip of cellular projections resembling microvilli and filopodia. In normal and infected cells these cellular projections contain actin microfilaments and in specialized retrovirus-tipped projections from the P815 cell, a direct association between actin filaments and the apical virus particle could be demonstrated (Mortara and Koch, 1986). Here we confirm and extend these observations using a murine macrophage cell line chronically infected with a C-type retrovirus. Immunochemical and biochemical methods were used to identify actin-associated and actin-binding components among the retroviral polypeptides. The results show that Pr65gag and its p15 N-terminal domain can bind to actin in vitro and may be major binding sites for actin filaments on the retroviral nucleocapsid.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="665af9f0099f25fe8e36c9420c3c5668" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407528,"asset_id":20503027,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407528/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503027"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503027"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503027; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503027]").text(description); $(".js-view-count[data-work-id=20503027]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503027; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503027']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "665af9f0099f25fe8e36c9420c3c5668" } } $('.js-work-strip[data-work-id=20503027]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503027,"title":"An association between actin and nucleocapsid polypeptides in isolated murine retroviral particles","internal_url":"https://www.academia.edu/20503027/An_association_between_actin_and_nucleocapsid_polypeptides_in_isolated_murine_retroviral_particles","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407528,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407528/thumbnails/1.jpg","file_name":"An_association_between_actin_and_nucleoc20160122-8918-13cdmrm.pdf","download_url":"https://www.academia.edu/attachments/41407528/download_file","bulk_download_file_name":"An_association_between_actin_and_nucleoc.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407528/An_association_between_actin_and_nucleoc20160122-8918-13cdmrm-libre.pdf?1453458874=\u0026response-content-disposition=attachment%3B+filename%3DAn_association_between_actin_and_nucleoc.pdf\u0026Expires=1739809200\u0026Signature=FFY4YlvSqXrQnHwT2WB8Qecqhdp24HY8qEeRnrrUjZMIeddeUHmksu5WmzZPP~qm63KQbwIExw9HGBdOuI22qFjp5D2a6NN-UrFZkkOhmiRAPMVZaHSkb6Klx4vey3x2Msa-M~CS2IMq~ha64H1A-W9JBhDTpSZMNQuyD5bDhLQ3aFDoQ7rxIwLYK-hbLtT09uFWMgWmj4xt1xu3SfCmpKARssEQky59Hmy8MEj7n75A~MHR9rAFNvWKvWfr~gVbsvbQ8X4z6Vk4vx39vj5S5wI5jbJ228Pxp3ckkVf~M0PEPfYEKNKgYr~D1Up4K0sIiv-2IXFfMs7CqFAlhRJhfw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503025"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503025/Aggregation_of_membrane_associated_actin_filaments_following_localized_adherence_of_enteropathogenic_Escherichia_coli_to_HeLa_cells"><img alt="Research paper thumbnail of Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells" class="work-thumbnail" src="https://attachments.academia-assets.com/41407543/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503025/Aggregation_of_membrane_associated_actin_filaments_following_localized_adherence_of_enteropathogenic_Escherichia_coli_to_HeLa_cells">Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells</a></div><div class="wp-workCard_item"><span>Journal of cell science</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have previously observed that enteropathogenic Escherichia coli (EPEC) adhere to HeLa cells in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have previously observed that enteropathogenic Escherichia coli (EPEC) adhere to HeLa cells in a localized manner, which we designated localized adherence as opposed to the diffuse pattern of adhesion. In this paper we have examined the effects of localized adherence of EPEC on the actin microfilament system of host HeLa cells. Centrifugation of bacteria onto HeLa cells improved the localized adherence and rapid rearrangements of actin filaments were detected by immunofluorescence and electron microscopy. Aggregation of microfilaments is consistently observed at the sites of localized adherence, and is abolished by cytochalasin D and low temperatures. Scanning electron microscopy indicates that these aggregates are surface microvilli entangled with attached EPEC.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e8021e0d56d4f9b184e81c1882cf8d00" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407543,"asset_id":20503025,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407543/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503025"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503025"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503025; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503025]").text(description); $(".js-view-count[data-work-id=20503025]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503025; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503025']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e8021e0d56d4f9b184e81c1882cf8d00" } } $('.js-work-strip[data-work-id=20503025]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503025,"title":"Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells","internal_url":"https://www.academia.edu/20503025/Aggregation_of_membrane_associated_actin_filaments_following_localized_adherence_of_enteropathogenic_Escherichia_coli_to_HeLa_cells","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407543,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407543/thumbnails/1.jpg","file_name":"Aggregation_of_membrane-associated_actin20160122-17374-oh49me.pdf","download_url":"https://www.academia.edu/attachments/41407543/download_file","bulk_download_file_name":"Aggregation_of_membrane_associated_actin.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407543/Aggregation_of_membrane-associated_actin20160122-17374-oh49me-libre.pdf?1453458875=\u0026response-content-disposition=attachment%3B+filename%3DAggregation_of_membrane_associated_actin.pdf\u0026Expires=1739809200\u0026Signature=ZiyS9sr7zWopoeRmQn~z8JOiND1it51t2L8Hb5Q9bF7NeKzXEcZIF4Iq5yNYCJGWjv-QuUo-lYeTbZw7umomBZQBODniPs7EG7GTUqpuB3GOAyBQPKqcvreFIqtwO8x7NpSnPdvCgR-nGClClrb-eHWYvtIsPWPUdYCK8LwU-bc7T00Qb6nP3oOhqe7aIGE7v2c1rLd0RnP4fXBxUvst9-g7HzCWWsReXfbynpsVEYA44oKmjglAluw5zCm~MfoENRuv0uWvTQsWLAskQxhiCDc178jrwSdvIdo7ozYJDMEjjbyz52WFfK0xFEf-HHotjgLWMC~N76bzATOfU7wpQg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="13368227"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13368227/Martin_BioChem_2010"><img alt="Research paper thumbnail of Martin-BioChem-2010" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/13368227/Martin_BioChem_2010">Martin-BioChem-2010</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ClovisNakaie">Clovis Nakaie</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SShimuta">S. Shimuta</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://unifesp.academia.edu/RENATOMORTARA">RENATO MORTARA</a></span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13368227"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13368227"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13368227; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13368227]").text(description); $(".js-view-count[data-work-id=13368227]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13368227; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13368227']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13368227]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13368227,"title":"Martin-BioChem-2010","internal_url":"https://www.academia.edu/13368227/Martin_BioChem_2010","owner_id":32599046,"coauthors_can_edit":true,"owner":{"id":32599046,"first_name":"Clovis","middle_initials":null,"last_name":"Nakaie","page_name":"ClovisNakaie","domain_name":"independent","created_at":"2015-06-27T21:23:19.425-07:00","display_name":"Clovis Nakaie","url":"https://independent.academia.edu/ClovisNakaie"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503021"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503021/Expression_inEscherichia_coli_ofa_Dominant_Immunogenof_Trypanosoma_cruzi_Recognized_byHumanChagasic_Sera"><img alt="Research paper thumbnail of Expression inEscherichia coli ofa Dominant Immunogenof Trypanosoma cruzi Recognized byHumanChagasic Sera" class="work-thumbnail" src="https://attachments.academia-assets.com/41407322/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503021/Expression_inEscherichia_coli_ofa_Dominant_Immunogenof_Trypanosoma_cruzi_Recognized_byHumanChagasic_Sera">Expression inEscherichia coli ofa Dominant Immunogenof Trypanosoma cruzi Recognized byHumanChagasic Sera</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A genomic clone expressing a Trypanosoma cruzi antigen in Escherichia coli was identified using h...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A genomic clone expressing a Trypanosoma cruzi antigen in Escherichia coli was identified using human chagasic sera. Chagasic antibodies affinity purified on extracts of this clone recognized a high-molecular-weight protein expressed in all developmental stages of the parasite life cycle, as well as in various T. cruzi strains. The antigen is associated with the cytoskeleton of the parasite and localizes along the attachment region between the flagellum and the cell body. Antibodies to the recombinant antigen were detected in the sera of 115 chagasic patients from different endemic regions, but not in sera of patients with leishmaniasis, T. rangeli infection, or other parasitic diseases. Our data suggest that the presence of antibodies to this antigen may be specifically associated with Chagas' disease.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ffe30e9e11f60e335ede7bb67df68cbb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407322,"asset_id":20503021,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407322/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503021"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503021"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503021; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503021]").text(description); $(".js-view-count[data-work-id=20503021]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503021; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503021']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ffe30e9e11f60e335ede7bb67df68cbb" } } $('.js-work-strip[data-work-id=20503021]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503021,"title":"Expression inEscherichia coli ofa Dominant Immunogenof Trypanosoma cruzi Recognized byHumanChagasic Sera","internal_url":"https://www.academia.edu/20503021/Expression_inEscherichia_coli_ofa_Dominant_Immunogenof_Trypanosoma_cruzi_Recognized_byHumanChagasic_Sera","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407322,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407322/thumbnails/1.jpg","file_name":"Cotrim-1990.pdf","download_url":"https://www.academia.edu/attachments/41407322/download_file","bulk_download_file_name":"Expression_inEscherichia_coli_ofa_Domina.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407322/Cotrim-1990-libre.pdf?1453458889=\u0026response-content-disposition=attachment%3B+filename%3DExpression_inEscherichia_coli_ofa_Domina.pdf\u0026Expires=1739809200\u0026Signature=YizhvZYGjxSp~swwgCc4ZVwgxamMnDBXq-x986wIqaiZm~uaGwpjxDEa6m6RCi~Fex566vHzjWpmRVtagGLD8S0ZTA2ZEyWNnOxURRpSXWRzVVyc-vOQhjCoYolY~pz56qlEBU9SerFX9apWy0Xo73bbvXXD~2W3vnRhYEJV7mOkQeTlQ75HPxbstGVzPrGDBwD9XQZEsf6iJh31lfLkk9ttWHRxjmGBPsBfzwaMd~HeKxGoX6pcqGC3Zv3DN-KYMaKxlgji0lMM8uZDTBzVgan1kjZ-ST1zGKcUWgN4fLacU~PBSTQ04z-WrakZW4E6TTpULGq-3yy6lx30VtoVpQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":41407321,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407321/thumbnails/1.jpg","file_name":"Cotrim-1990.pdf","download_url":"https://www.academia.edu/attachments/41407321/download_file","bulk_download_file_name":"Expression_inEscherichia_coli_ofa_Domina.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407321/Cotrim-1990-libre.pdf?1453458889=\u0026response-content-disposition=attachment%3B+filename%3DExpression_inEscherichia_coli_ofa_Domina.pdf\u0026Expires=1739809200\u0026Signature=NbUkgKKBwoWI9~P2fUOMVBq8zuIoqvAeKlMORFSpngEDrD4btRQULUNJzXPcXjCqzXUoi2yF~je-1HO9gwXIOWGvz3pMSP~F3r8sJv7~gdGWTrniyH7STfwqiRs0xuD8-ZnqSxOhpDpVzCfq-~muf7y2LDg~onRTWsIWxnufg8K3WQ8y-h7Sd6JdsEv7IFjFPcR9SWESRJydCA3IS3j~MErv9oPDjPVquzyTB9-BzuBMQMWLpN83tlvfqNce7L1eY2Umgj7A6gE2~pX8eI1d04saZnaZ7Cm46a1LEpuqkvhVk~ko1Pa7QyO0fNUftfwiNqo8YjJ1IKolWEuo2jcOvQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503018"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503018/Confocal_fluorescence_microscopy_a_powerful_tool_in_the_study_of_Chagas_disease"><img alt="Research paper thumbnail of Confocal fluorescence microscopy: a powerful tool in the study of Chagas' disease" class="work-thumbnail" src="https://attachments.academia-assets.com/41407515/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503018/Confocal_fluorescence_microscopy_a_powerful_tool_in_the_study_of_Chagas_disease">Confocal fluorescence microscopy: a powerful tool in the study of Chagas' disease</a></div><div class="wp-workCard_item"><span>Revista da Sociedade Brasileira de Medicina Tropical</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Confocal scanning fluorescence microscopy has become widely used in cell biology and pathology. I...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Confocal scanning fluorescence microscopy has become widely used in cell biology and pathology. In conjunction with monoclonal antibodies it may turn out to be a powerful diagnostic tool that also enables detailed studies of tissue forms of Trypanosoma cruzi.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="343d7648390a7a0b24924bc185a51410" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407515,"asset_id":20503018,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407515/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503018"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503018"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503018; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503018]").text(description); $(".js-view-count[data-work-id=20503018]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503018; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503018']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "343d7648390a7a0b24924bc185a51410" } } $('.js-work-strip[data-work-id=20503018]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503018,"title":"Confocal fluorescence microscopy: a powerful tool in the study of Chagas' disease","internal_url":"https://www.academia.edu/20503018/Confocal_fluorescence_microscopy_a_powerful_tool_in_the_study_of_Chagas_disease","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407515,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407515/thumbnails/1.jpg","file_name":"Confocal_fluorescence_microscopy_a_power20160122-17380-oh6mnq.pdf","download_url":"https://www.academia.edu/attachments/41407515/download_file","bulk_download_file_name":"Confocal_fluorescence_microscopy_a_power.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407515/Confocal_fluorescence_microscopy_a_power20160122-17380-oh6mnq-libre.pdf?1453458875=\u0026response-content-disposition=attachment%3B+filename%3DConfocal_fluorescence_microscopy_a_power.pdf\u0026Expires=1739809200\u0026Signature=GvIhIUlsO6wB8tnZvX9x4WIftmt4RLVzGnSFMfy-v01RWVi-ETkrUDB8nSABhFrFI47dp~aiWdAlk6xMLwgXdwoanD8HVb5SNyUbSvrL-6In9IlNSK7fEYVGWZwYNIyMNlLN3~GvxQrGDvSrKu~kz-5zEwlCOHHM9vKYrZvgQRpj2r6uSjgqpGNGCCVGjMzZQRvJ9YGWuvx7n74aYajjyYSJ4LyyTbR7B7oiMZphF4nSWCtDYyw6~y~ABcGZj9zSsBpUICjVpnNj0MvHBZwx8ftDc0DLKKVWKB7eAZi~DQ95NXabVkCbAS65kjLhdD8Kq5hPMyWiMPpLB7M9DWIpXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503016"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503016/T_cell_subpopulations_in_myocardial_inflammatory_infiltrates_detected_by_confocal_microscopy_dose_dependence_in_mice_treated_with_cyclophosphamide_during_acute_Trypanosoma_cruziinfection_D%C3%A9tection_de_sous_populations_de_cellules_T_dans_les_infiltrats_inflammatoire_myocardiques_par_microscopie_co_"><img alt="Research paper thumbnail of T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruziinfection Détection de sous-populations de cellules T dans les infiltrats inflammatoire myocardiques par microscopie co..." class="work-thumbnail" src="https://attachments.academia-assets.com/41407324/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503016/T_cell_subpopulations_in_myocardial_inflammatory_infiltrates_detected_by_confocal_microscopy_dose_dependence_in_mice_treated_with_cyclophosphamide_during_acute_Trypanosoma_cruziinfection_D%C3%A9tection_de_sous_populations_de_cellules_T_dans_les_infiltrats_inflammatoire_myocardiques_par_microscopie_co_">T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruziinfection Détection de sous-populations de cellules T dans les infiltrats inflammatoire myocardiques par microscopie co...</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In this article, we have characterized cell subpopulations found in the hearts of mice presenting...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In this article, we have characterized cell subpopulations found in the hearts of mice presenting acute Chagas&#39; disease by immunocy- tochemistry and subjected to different schedules of an immunosuppressive therapy with cyclophosphamide (CY). In this comparative study, CY treatment with different doses was carried out before or after infection with Trypanosoma cruziY strain trypomastigotes, enabling us to discriminate the parasitemic</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="187f062ad78531e3b2279e4db95e8a7e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407324,"asset_id":20503016,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407324/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503016"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503016"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503016; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503016]").text(description); $(".js-view-count[data-work-id=20503016]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503016; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503016']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "187f062ad78531e3b2279e4db95e8a7e" } } $('.js-work-strip[data-work-id=20503016]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503016,"title":"T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruziinfection Détection de sous-populations de cellules T dans les infiltrats inflammatoire myocardiques par microscopie co...","internal_url":"https://www.academia.edu/20503016/T_cell_subpopulations_in_myocardial_inflammatory_infiltrates_detected_by_confocal_microscopy_dose_dependence_in_mice_treated_with_cyclophosphamide_during_acute_Trypanosoma_cruziinfection_D%C3%A9tection_de_sous_populations_de_cellules_T_dans_les_infiltrats_inflammatoire_myocardiques_par_microscopie_co_","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407324,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407324/thumbnails/1.jpg","file_name":"Calabrese-2003a.pdf","download_url":"https://www.academia.edu/attachments/41407324/download_file","bulk_download_file_name":"T_cell_subpopulations_in_myocardial_infl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407324/Calabrese-2003a-libre.pdf?1453458889=\u0026response-content-disposition=attachment%3B+filename%3DT_cell_subpopulations_in_myocardial_infl.pdf\u0026Expires=1739809200\u0026Signature=YaL0xP2pQ4rqjm8zm~Qg2WLP7dNRAMeIYgDo1DSHhb4XGE43uJvIIiCERVW7MqQhZ7E2ofht4oswAC5OfNvgd5CY0wElcrJi73d23FM9elgyXkxtdKIn1oRl-Oj6pgRsehfSLAFL2~TDoTxDHN8gpTkbDTc84YrkQjPpebTOkHtYX52nDYG0XCbAjB07Llux5sXAvKUROezPI6rePXxgry1b-BmSKiMV0DKe7ew3ihWIKUNzCV4A3Rii5AGanjCvkL~MZDeWQEwTmduZwoeljDoK0defUy16zD9jGTWREQuvLdr9u5U8jT7JZGOUPQtOYvg9MkrOcK6afQI9~cKHDQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="20503013"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/20503013/Study_of_acute_chagasic_mice_under_immunosuppressive_therapy_by_cyclosporin_A_modulation_and_confocal_analysis_of_inflammatory_reaction"><img alt="Research paper thumbnail of Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction" class="work-thumbnail" src="https://attachments.academia-assets.com/41407500/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/20503013/Study_of_acute_chagasic_mice_under_immunosuppressive_therapy_by_cyclosporin_A_modulation_and_confocal_analysis_of_inflammatory_reaction">Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction</a></div><div class="wp-workCard_item"><span>Immunopharmacology</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">. The in vivo effects of cyclosporin A CsA on Trypanosoma cruzi infection were examined using dif...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">. The in vivo effects of cyclosporin A CsA on Trypanosoma cruzi infection were examined using different schedules of the drug in mice infected with the Y strain. Parasitaemia at day 8 after infection among CsA-treated animals was usually higher than control infected non-treated mice. On the other hand, mortality analysis showed that animals CsA-treated either Ž . with 200 mgrkg 2 days before infection or with therapeutic doses 10 mgrkg every other day showed almost the same Ž . mean time of death 35.8 and 38.2 days, respectively . In these groups mice died 50% less than control infected non-treated ones. The mean time of death in the animals treated with 200 mgrkg 5 days after infection and in infected non-treated control mice were respectively 29.0 and 22.6 days. The kinetics analysis of the leukocyte population of animals treated with a single dose of 200 mgrkg of CsA before or after infection did not show the alternate pattern of leukopeniarleukocytosis observed in control groups of infected mice but differential cell counts indicated a modulatory action upon circulating leukocytes of therapeutic doses of CsA. The animals treated with any of the CsA schedules showed a moderate to intense diffuse inflammatory reaction exhibiting mainly mononuclear cells in the heart. Immunofluorescence analysis by confocal microscopy revealed that macrophages are a major component of the inflammatory infiltrate in all groups of CsA-treated mice and also in the control group. q</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="151df4891dcc204727223479f607c36e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":41407500,"asset_id":20503013,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/41407500/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="20503013"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="20503013"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20503013; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20503013]").text(description); $(".js-view-count[data-work-id=20503013]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20503013; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='20503013']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "151df4891dcc204727223479f607c36e" } } $('.js-work-strip[data-work-id=20503013]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":20503013,"title":"Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A: modulation and confocal analysis of inflammatory reaction","internal_url":"https://www.academia.edu/20503013/Study_of_acute_chagasic_mice_under_immunosuppressive_therapy_by_cyclosporin_A_modulation_and_confocal_analysis_of_inflammatory_reaction","owner_id":92671,"coauthors_can_edit":true,"owner":{"id":92671,"first_name":"RENATO","middle_initials":null,"last_name":"MORTARA","page_name":"RENATOMORTARA","domain_name":"unifesp","created_at":"2009-11-30T19:37:15.402-08:00","display_name":"RENATO MORTARA","url":"https://unifesp.academia.edu/RENATOMORTARA"},"attachments":[{"id":41407500,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/41407500/thumbnails/1.jpg","file_name":"Calabrese-2000.pdf","download_url":"https://www.academia.edu/attachments/41407500/download_file","bulk_download_file_name":"Study_of_acute_chagasic_mice_under_immun.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/41407500/Calabrese-2000-libre.pdf?1453458878=\u0026response-content-disposition=attachment%3B+filename%3DStudy_of_acute_chagasic_mice_under_immun.pdf\u0026Expires=1739809200\u0026Signature=d5UW3wKAh5vU4oRwLAtf7~SbC5ed-ogMjv38hVa~3BNtUNgYaq3YjFFXOkwFy-CEDrV4tzKfTcmftmzqpp6tUUgkFurwe3ang9r1JuisSqs9n6gAbytcVLfPby7KrOUd7mrX0uFHeVDhEYO9KKqAYN4MCGD6h8NKlsWCR5RNprBMgNXllevN6OR~qXBxfc80hnY~PSQG6KmvTX4iPjANBiVzYlPB1siG3uKusTAsc8K7weXOr0T-nm1j4bg4P8dW5ugAGyjWCK60mAm3v7LaD1C86SIKJi2xSH8cx3Pmyu0GcRbZhb8Zp0AMaAECUlGP6OV~hfhLHO7jcCpt4LnVog__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/google_contacts-0dfb882d836b94dbcb4a2d123d6933fc9533eda5be911641f20b4eb428429600.js"], function() { // from javascript_helper.rb $('.js-google-connect-button').click(function(e) { e.preventDefault(); GoogleContacts.authorize_and_show_contacts(); Aedu.Dismissibles.recordClickthrough("WowProfileImportContactsPrompt"); }); $('.js-update-biography-button').click(function(e) { e.preventDefault(); Aedu.Dismissibles.recordClickthrough("UpdateUserBiographyPrompt"); $.ajax({ url: $r.api_v0_profiles_update_about_path({ subdomain_param: 'api', about: "", }), type: 'PUT', success: function(response) { location.reload(); } }); }); $('.js-work-creator-button').click(function (e) { e.preventDefault(); window.location = $r.upload_funnel_document_path({ source: encodeURIComponent(""), }); }); $('.js-video-upload-button').click(function (e) { e.preventDefault(); window.location = $r.upload_funnel_video_path({ source: encodeURIComponent(""), }); }); $('.js-do-this-later-button').click(function() { $(this).closest('.js-profile-nag-panel').remove(); Aedu.Dismissibles.recordDismissal("WowProfileImportContactsPrompt"); }); $('.js-update-biography-do-this-later-button').click(function(){ $(this).closest('.js-profile-nag-panel').remove(); Aedu.Dismissibles.recordDismissal("UpdateUserBiographyPrompt"); }); $('.wow-profile-mentions-upsell--close').click(function(){ $('.wow-profile-mentions-upsell--panel').hide(); Aedu.Dismissibles.recordDismissal("WowProfileMentionsUpsell"); }); $('.wow-profile-mentions-upsell--button').click(function(){ Aedu.Dismissibles.recordClickthrough("WowProfileMentionsUpsell"); }); new WowProfile.SocialRedesignUserWorks({ initialWorksOffset: 20, allWorksOffset: 20, maxSections: 1 }) }); </script> </div></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile_edit-5ea339ee107c863779f560dd7275595239fed73f1a13d279d2b599a28c0ecd33.js","https://a.academia-assets.com/assets/add_coauthor-22174b608f9cb871d03443cafa7feac496fb50d7df2d66a53f5ee3c04ba67f53.js","https://a.academia-assets.com/assets/tab-dcac0130902f0cc2d8cb403714dd47454f11fc6fb0e99ae6a0827b06613abc20.js","https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js"], function() { // from javascript_helper.rb window.ae = window.ae || {}; window.ae.WowProfile = window.ae.WowProfile || {}; if(Aedu.User.current && Aedu.User.current.id === $viewedUser.id) { window.ae.WowProfile.current_user_edit = {}; new WowProfileEdit.EditUploadView({ el: '.js-edit-upload-button-wrapper', model: window.$current_user, }); new AddCoauthor.AddCoauthorsController(); } var userInfoView = new WowProfile.SocialRedesignUserInfo({ recaptcha_key: "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB" }); WowProfile.router = new WowProfile.Router({ userInfoView: userInfoView }); Backbone.history.start({ pushState: true, root: "/" + $viewedUser.page_name }); new WowProfile.UserWorksNav() }); </script> </div> <div class="bootstrap login"><div class="modal fade login-modal" id="login-modal"><div class="login-modal-dialog modal-dialog"><div class="modal-content"><div class="modal-header"><button class="close close" data-dismiss="modal" type="button"><span aria-hidden="true">×</span><span class="sr-only">Close</span></button><h4 class="modal-title text-center"><strong>Log In</strong></h4></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><button class="btn btn-fb btn-lg btn-block btn-v-center-content" id="login-facebook-oauth-button"><svg style="float: left; width: 19px; line-height: 1em; margin-right: .3em;" aria-hidden="true" focusable="false" data-prefix="fab" data-icon="facebook-square" class="svg-inline--fa fa-facebook-square fa-w-14" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path fill="currentColor" d="M400 32H48A48 48 0 0 0 0 80v352a48 48 0 0 0 48 48h137.25V327.69h-63V256h63v-54.64c0-62.15 37-96.48 93.67-96.48 27.14 0 55.52 4.84 55.52 4.84v61h-31.27c-30.81 0-40.42 19.12-40.42 38.73V256h68.78l-11 71.69h-57.78V480H400a48 48 0 0 0 48-48V80a48 48 0 0 0-48-48z"></path></svg><small><strong>Log in</strong> with <strong>Facebook</strong></small></button><br /><button class="btn btn-google btn-lg btn-block btn-v-center-content" id="login-google-oauth-button"><svg style="float: left; width: 22px; line-height: 1em; margin-right: .3em;" aria-hidden="true" focusable="false" data-prefix="fab" data-icon="google-plus" class="svg-inline--fa fa-google-plus fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M256,8C119.1,8,8,119.1,8,256S119.1,504,256,504,504,392.9,504,256,392.9,8,256,8ZM185.3,380a124,124,0,0,1,0-248c31.3,0,60.1,11,83,32.3l-33.6,32.6c-13.2-12.9-31.3-19.1-49.4-19.1-42.9,0-77.2,35.5-77.2,78.1S142.3,334,185.3,334c32.6,0,64.9-19.1,70.1-53.3H185.3V238.1H302.2a109.2,109.2,0,0,1,1.9,20.7c0,70.8-47.5,121.2-118.8,121.2ZM415.5,273.8v35.5H380V273.8H344.5V238.3H380V202.8h35.5v35.5h35.2v35.5Z"></path></svg><small><strong>Log in</strong> with <strong>Google</strong></small></button><br /><style type="text/css">.sign-in-with-apple-button { width: 100%; height: 52px; border-radius: 3px; border: 1px solid black; cursor: pointer; } .sign-in-with-apple-button > div { margin: 0 auto; / This centers the Apple-rendered button horizontally }</style><script src="https://appleid.cdn-apple.com/appleauth/static/jsapi/appleid/1/en_US/appleid.auth.js" type="text/javascript"></script><div class="sign-in-with-apple-button" data-border="false" data-color="white" id="appleid-signin"><span   ="Sign Up with Apple" class="u-fs11"></span></div><script>AppleID.auth.init({ clientId: 'edu.academia.applesignon', scope: 'name email', redirectURI: 'https://www.academia.edu/sessions', state: "4c1fb367fc0827af219126aaef784c16ab8cbac30a8e4a375a84a19bdebb8cd0", });</script><script>// Hacky way of checking if on fast loswp if (window.loswp == null) { (function() { const Google = window?.Aedu?.Auth?.OauthButton?.Login?.Google; const Facebook = window?.Aedu?.Auth?.OauthButton?.Login?.Facebook; if (Google) { new Google({ el: '#login-google-oauth-button', rememberMeCheckboxId: 'remember_me', track: null }); } if (Facebook) { new Facebook({ el: '#login-facebook-oauth-button', rememberMeCheckboxId: 'remember_me', track: null }); } })(); }</script></div></div></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><div class="hr-heading login-hr-heading"><span class="hr-heading-text">or</span></div></div></div></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><form class="js-login-form" action="https://www.academia.edu/sessions" accept-charset="UTF-8" method="post"><input type="hidden" name="authenticity_token" value="SCQNkmH7xxvhmdDwZ9-HMt19nT4ZblGoaq_kLPYb8aiXbOXJ9AblgJ6o9Pp3322FPOvTGEIhcmcWfyYttldVrA" autocomplete="off" /><div class="form-group"><label class="control-label" for="login-modal-email-input" style="font-size: 14px;">Email</label><input class="form-control" id="login-modal-email-input" name="login" type="email" /></div><div class="form-group"><label class="control-label" for="login-modal-password-input" style="font-size: 14px;">Password</label><input class="form-control" id="login-modal-password-input" name="password" type="password" /></div><input type="hidden" name="post_login_redirect_url" id="post_login_redirect_url" value="https://unifesp.academia.edu/RENATOMORTARA" autocomplete="off" /><div class="checkbox"><label><input type="checkbox" name="remember_me" id="remember_me" value="1" checked="checked" /><small style="font-size: 12px; margin-top: 2px; display: inline-block;">Remember me on this computer</small></label></div><br><input type="submit" name="commit" value="Log In" class="btn btn-primary btn-block btn-lg js-login-submit" data-disable-with="Log In" /></br></form><script>typeof window?.Aedu?.recaptchaManagedForm === 'function' && window.Aedu.recaptchaManagedForm( document.querySelector('.js-login-form'), document.querySelector('.js-login-submit') );</script><small style="font-size: 12px;"><br />or <a data-target="#login-modal-reset-password-container" data-toggle="collapse" href="javascript:void(0)">reset password</a></small><div class="collapse" id="login-modal-reset-password-container"><br /><div class="well margin-0x"><form class="js-password-reset-form" action="https://www.academia.edu/reset_password" accept-charset="UTF-8" method="post"><input type="hidden" name="authenticity_token" value="1AhraT64PhyfQ34JvHcr-UjaQVfo0kWu7yu6p6qeFbULQIMyq0Uch-ByWgOsd8FOqUwPcbOdZmGT-3im6tKxsQ" autocomplete="off" /><p>Enter the email address you signed up with and we'll email you a reset link.</p><div class="form-group"><input class="form-control" name="email" type="email" /></div><script src="https://recaptcha.net/recaptcha/api.js" async defer></script> <script> var invisibleRecaptchaSubmit = function () { var closestForm = function (ele) { var curEle = ele.parentNode; while (curEle.nodeName !== 'FORM' && curEle.nodeName !== 'BODY'){ curEle = curEle.parentNode; } return curEle.nodeName === 'FORM' ? curEle : null }; var eles = document.getElementsByClassName('g-recaptcha'); if (eles.length > 0) { var form = closestForm(eles[0]); if (form) { form.submit(); } } }; </script> <input type="submit" data-sitekey="6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj" data-callback="invisibleRecaptchaSubmit" class="g-recaptcha btn btn-primary btn-block" value="Email me a link" value=""/> </form></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/collapse-45805421cf446ca5adf7aaa1935b08a3a8d1d9a6cc5d91a62a2a3a00b20b3e6a.js"], function() { // from javascript_helper.rb $("#login-modal-reset-password-container").on("shown.bs.collapse", function() { $(this).find("input[type=email]").focus(); }); }); </script> </div></div></div><div class="modal-footer"><div class="text-center"><small style="font-size: 12px;">Need an account? <a rel="nofollow" href="https://www.academia.edu/signup">Click here to sign up</a></small></div></div></div></div></div></div><script>// If we are on subdomain or non-bootstrapped page, redirect to login page instead of showing modal (function(){ if (typeof $ === 'undefined') return; var host = window.location.hostname; if ((host === $domain || host === "www."+$domain) && (typeof $().modal === 'function')) { $("#nav_log_in").click(function(e) { // Don't follow the link and open the modal e.preventDefault(); $("#login-modal").on('shown.bs.modal', function() { $(this).find("#login-modal-email-input").focus() }).modal('show'); }); } })()</script> <div class="bootstrap" id="footer"><div class="footer-content clearfix text-center padding-top-7x" style="width:100%;"><ul class="footer-links-secondary footer-links-wide list-inline margin-bottom-1x"><li><a href="https://www.academia.edu/about">About</a></li><li><a href="https://www.academia.edu/press">Press</a></li><li><a href="https://www.academia.edu/documents">Papers</a></li><li><a href="https://www.academia.edu/topics">Topics</a></li><li><a href="https://www.academia.edu/journals">Academia.edu Journals</a></li><li><a rel="nofollow" href="https://www.academia.edu/hiring"><svg style="width: 13px; height: 13px;" aria-hidden="true" focusable="false" data-prefix="fas" data-icon="briefcase" class="svg-inline--fa fa-briefcase fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M320 336c0 8.84-7.16 16-16 16h-96c-8.84 0-16-7.16-16-16v-48H0v144c0 25.6 22.4 48 48 48h416c25.6 0 48-22.4 48-48V288H320v48zm144-208h-80V80c0-25.6-22.4-48-48-48H176c-25.6 0-48 22.4-48 48v48H48c-25.6 0-48 22.4-48 48v80h512v-80c0-25.6-22.4-48-48-48zm-144 0H192V96h128v32z"></path></svg> <strong>We're Hiring!</strong></a></li><li><a rel="nofollow" href="https://support.academia.edu/hc/en-us"><svg style="width: 12px; height: 12px;" aria-hidden="true" focusable="false" data-prefix="fas" data-icon="question-circle" class="svg-inline--fa fa-question-circle fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M504 256c0 136.997-111.043 248-248 248S8 392.997 8 256C8 119.083 119.043 8 256 8s248 111.083 248 248zM262.655 90c-54.497 0-89.255 22.957-116.549 63.758-3.536 5.286-2.353 12.415 2.715 16.258l34.699 26.31c5.205 3.947 12.621 3.008 16.665-2.122 17.864-22.658 30.113-35.797 57.303-35.797 20.429 0 45.698 13.148 45.698 32.958 0 14.976-12.363 22.667-32.534 33.976C247.128 238.528 216 254.941 216 296v4c0 6.627 5.373 12 12 12h56c6.627 0 12-5.373 12-12v-1.333c0-28.462 83.186-29.647 83.186-106.667 0-58.002-60.165-102-116.531-102zM256 338c-25.365 0-46 20.635-46 46 0 25.364 20.635 46 46 46s46-20.636 46-46c0-25.365-20.635-46-46-46z"></path></svg> <strong>Help Center</strong></a></li></ul><ul class="footer-links-tertiary list-inline margin-bottom-1x"><li class="small">Find new research papers in:</li><li class="small"><a href="https://www.academia.edu/Documents/in/Physics">Physics</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Chemistry">Chemistry</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Biology">Biology</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Health_Sciences">Health Sciences</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Ecology">Ecology</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Earth_Sciences">Earth Sciences</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Cognitive_Science">Cognitive Science</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Mathematics">Mathematics</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Computer_Science">Computer Science</a></li></ul></div></div><div class="DesignSystem" id="credit" style="width:100%;"><ul class="u-pl0x footer-links-legal list-inline"><li><a rel="nofollow" href="https://www.academia.edu/terms">Terms</a></li><li><a rel="nofollow" href="https://www.academia.edu/privacy">Privacy</a></li><li><a rel="nofollow" href="https://www.academia.edu/copyright">Copyright</a></li><li>Academia ©2025</li></ul></div><script> //<![CDATA[ window.detect_gmtoffset = true; window.Academia && window.Academia.set_gmtoffset && Academia.set_gmtoffset('/gmtoffset'); //]]> </script> <div id='overlay_background'></div> <div id='bootstrap-modal-container' class='bootstrap'></div> <div id='ds-modal-container' class='bootstrap DesignSystem'></div> <div id='full-screen-modal'></div> </div> </body> </html>