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Magic™ Cell-Free Membrane Protein Expression Kits
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Creative Biolabs has developed a series of cell-free expression systems for high-quality, large/medium-scale membrane protein expression. We provide the most advanced Cell-free Membrane Protein expression kits for our global clients. </p> <h2 class="tsponav_tit"> <em>E. coli</em> Lysate </h2> <ul class="ullist"> <li> • Facilitate the production of target proteins in high levels </li> <li> • Simple protocol and easy setup with all necessary components </li> <li> • The target proteins can be used in various downstream applications </li> <li> • Protein of interest can be synthesized and visualized in a few hours </li> <li> • Low cost </li> </ul> <br /> <div class="table-responsive"> <table cellspacing="0" cellpadding="0" class="table table-striped table-bordered"> <thead> <tr class="active"> <td width="15%"> <strong>Cat</strong> </td> <td width="60%"> <strong>Product Name</strong> </td> <td width="25%"> <strong>Expected Yield of Positive Control</strong> </td> </tr> </thead> <tbody> <tr> <td> <a href="magic-cell-free-protein-expression-in-e-coli-lysate-18000.htm">MPCFK-1</a> </td> <td> Magic™ Cell-free Membrane Protein Expression in <em>E. coli</em> Lysate </td> <td> 0.75 mg/ml </td> </tr> <tr> <td> <a href="magic-simplified-cell-free-protein-expression-in-e-coli-lysate-18001.htm">MPCFK-1-1</a> </td> <td> Magic™ Simplified Cell-free Membrane Protein Expression in <em>E. coli</em> Lysate </td> <td> 0.45 mg/ml </td> </tr> <tr> <td> <a href="magic-cell-free-protein-expression-in-e-coli-lysate-dialysis-mode-18002.htm">MPCFK-1-2</a> </td> <td> Magic™ Cell-free Membrane Protein Expression in <em>E. coli</em> Lysate - Dialysis Mode </td> <td> 5-10 mg/ml </td> </tr> </tbody> </table> </div> <h2 class="tsponav_tit"> Mammalian Cell Lysate </h2> <ul class="ullist"> <li> • Synthesized in as little as 1 hour and easy setup with all necessary components in a single-tube reaction </li> <li> • Provides high yield and functional protein </li> <li> • Enables the synthesis of large proteins (such as over 150 kDa) </li> <li> • Modifications such as glycosylation and phosphorylation could be easily obtained </li> <li> • Translation enhancement factor included in the Accessory Proteins ensures the high translation level </li> <li> • Various applications: high throughput screening, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity, and protein-protein interactions </li> </ul> <br /> <div class="table-responsive"> <table cellspacing="0" cellpadding="0" class="table table-striped table-bordered"> <thead> <tr class="active"> <td width="15%"> <strong>Cat</strong> </td> <td width="60%"> <strong>Product Name</strong> </td> <td width="25%"> <strong>Expected Yield of Positive Control</strong> </td> </tr> </thead> <tbody> <tr> <td> <a href="magic-cell-free-protein-expression-in-mammalian-cell-lysate-18003.htm">MPCFK-2</a> </td> <td> Magic™ Cell-free Membrane Protein Expression in Mammalian Cell Lysate </td> <td> 0.75 mg/ml </td> </tr> <tr> <td> <a href="magic-cell-free-protein-expression-in-human-hela-cell-lysate-18004.htm">MPCFK-2-2 </a> </td> <td> Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate </td> <td> 0.1 mg/ml </td> </tr> </tbody> </table> </div> <h2 class="tsponav_tit"> Wheat Germ Lysate </h2> <ul class="ullist"> <li> • Membrane protein has been synthesized using proteoliposome </li> <li> • No need to prepare reagents </li> <li> • Reagents necessary for each reaction are already pre-packaged in tubes </li> <li> • Synthesize proteins with just a few pipetting steps </li> <li> • Target proteins can be confirmed through SDS-PAGE/CBB staining </li> </ul> <br /> <div class="table-responsive"> <table cellspacing="0" cellpadding="0" class="table table-striped table-bordered"> <thead> <tr class="active"> <td width="15%"> <strong>Cat</strong> </td> <td width="60%"> <strong>Product Name</strong> </td> <td width="25%"> <strong>Expected Yield of Positive Control</strong> </td> </tr> </thead> <tbody> <tr> <td> <a href="magic-cell-free-protein-expression-in-wheat-germ-lysate-with-liposome-18005.htm">MPCFK-3 </a> </td> <td> Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate with liposome </td> <td> 40 µg/ml </td> </tr> <tr> <td> <a href="magic-cell-free-protein-expression-in-wheat-germ-lysate-18006.htm">MPCFK-3-1</a> </td> <td> Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate </td> <td> 0.1-0.2 mg/ml </td> </tr> <tr> <td> <a href="magic-cell-free-protein-expression-in-wheat-germ-lysate-for-protein-expression-screening-18007.htm">MPCFK-3-2 </a> </td> <td> Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate for membrane protein expression screening </td> <td> 20 µg/ml </td> </tr> <tr> <td> <a href="magic-cell-free-protein-expression-in-wheat-germ-lysate-bilayer-format-18008.htm">MPCFK-3-3</a> </td> <td> Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate - Bilayer Format </td> <td> 20-40 µg/ml </td> </tr> <tr> <td> <a href="magic-small-scale-cell-free-protein-expression-in-wheat-germ-lysate-for-protein-production-18009.htm">MPCFK-3-4</a> </td> <td> Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for Protein Production </td> <td> 20-40 µg/ml </td> </tr> <tr> <td> <a href="magic-small-scale-cell-free-protein-expression-in-wheat-germ-lysate-for-gst-tagged-protein-production-18010.htm">MPCFK-3-5 </a> </td> <td> Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for GST-tagged Protein Production </td> <td> 20-40 µg/ml </td> </tr> <tr> <td> <a href="magic-small-scale-cell-free-protein-expression-in-wheat-germ-lysate-for-his-tagged-protein-production-18011.htm">MPCFK-3-6 </a> </td> <td> Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for His-tagged Protein Production </td> <td> 20-40 µg/ml </td> </tr> <tr> <td colspan="3"> <p align="center"> <strong><a href="search.aspx?key=Wheat Germ lysate&ty=tag">Search for More Cell-free Membrane Protein Expression Kits in Wheat Germ Lysate </a></strong> </p> </td> </tr> </tbody> </table> </div> <h2 class="tsponav_tit"> Insect Cell Lysate </h2> <ul class="ullist"> <li> • Convenient, quick, single-tube, coupled transcription/translation system </li> <li> • Allows both transcription and translation to occur in a single reaction </li> <li> • Can be scaled by changing the component volumes proportionally </li> <li> • T7 RNA polymerase promoter </li> <li> • Addition of labels to the reaction </li> </ul> <br /> <div class="table-responsive"> <table cellspacing="0" cellpadding="0" class="table table-striped table-bordered"> <thead> <tr class="active"> <td width="15%"> <strong>Cat</strong> </td> <td width="60%"> <strong>Product Name</strong> </td> <td width="25%"> <strong>Expected Yield of Positive Control</strong> </td> </tr> </thead> <tbody> <tr> <td> <a href="magic-cell-free-protein-expression-in-insect-cell-lysate-18028.htm">MPCFK-5</a> </td> <td> Magic™ Cell-free Membrane Protein Expression in Insect Cell Lysate </td> <td> 0.1 mg/ml </td> </tr> </tbody> </table> </div> <h2 class="tsponav_tit"> Rabbit Reticulocyte Lysate </h2> <ul class="ullist"> <li> • Compared with conventional cell-based/Cell-free Membrane Protein synthesis platforms, RRL system exerts multiple superior features: </li> <li> • Easily prepared </li> <li> • Cap-independent translation </li> <li> • Compatible with mammalian post-translational protein modification system </li> <li> • Large proteins greater than 30 kDa can be expressed efficiently </li> <li> • Compatible with high-throughput format for protein microarrays and other screening procedures </li> </ul> <br /> <div class="table-responsive"> <table cellspacing="0" cellpadding="0" class="table table-striped table-bordered"> <thead> <tr class="active"> <td width="15%"> <strong>Cat</strong> </td> <td width="60%"> <strong>Product Name</strong> </td> <td width="25%"> <strong>Expected Yield of Positive Control</strong> </td> </tr> </thead> <tbody> <tr> <td> <a href="magic-cell-free-protein-expression-in-rabbit-reticulocyte-lysate-18029.htm">MPCFK-6</a> </td> <td> Magic™ Cell-free Membrane Protein Expression in Rabbit Reticulocyte Lysate </td> <td> Based on RNA purity </td> </tr> </tbody> </table> </div> <h2 class="tsponav_tit"> Plant Cell Lysate </h2> <ul class="ullist"> <li> • High-yield: Up to 3 mg/mL expressed protein </li> <li> • Easy to use: One-step reaction protocol </li> <li> • Fast: High yield in just 48 hours </li> <li> • Flexible: Express "difficult to produce" proteins, cytotoxic proteins, <em>etc</em>. </li> <li> • Convenience: suitable for various membrane protein expressions </li> </ul> <br /> <div class="table-responsive"> <table cellspacing="0" cellpadding="0" class="table table-striped table-bordered"> <thead> <tr class="active"> <td width="15%"> <strong>Cat</strong> </td> <td width="60%"> <strong>Product Name</strong> </td> <td width="25%"> <strong>Expected Yield of Positive Control</strong> </td> </tr> </thead> <tbody> 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