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Kiril i Metodij" - Skopje)</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a data-nosnippet="" href="https://unifi.academia.edu/AndreaNovelli"><img class="profile-avatar u-positionAbsolute" alt="Andrea Novelli related author profile picture" border="0" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://unifi.academia.edu/AndreaNovelli">Andrea Novelli</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Università degli Studi di Firenze (University of Florence)</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a data-nosnippet="" href="https://independent.academia.edu/WReygaert"><img class="profile-avatar u-positionAbsolute" alt="Wanda Reygaert related author profile picture" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/195827923/58161432/46389117/s200_wanda.reygaert.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/WReygaert">Wanda Reygaert</a></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a data-nosnippet="" href="https://independent.academia.edu/RonaldJones12"><img class="profile-avatar u-positionAbsolute" alt="Ronald Jones related author profile picture" border="0" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/RonaldJones12">Ronald Jones</a></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a data-nosnippet="" href="https://gkpublication.academia.edu/InternationalJournalofResearchReview"><img class="profile-avatar u-positionAbsolute" alt="International Journal of Research & Review (IJRR) related author profile picture" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/17398118/35115993/30587588/s200_international_journal_of.research_review_ijrr_.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://gkpublication.academia.edu/InternationalJournalofResearchReview">International Journal of Research & Review (IJRR)</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">GKP</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a data-nosnippet="" href="https://independent.academia.edu/DavidTalan"><img class="profile-avatar u-positionAbsolute" alt="David Talan related author profile picture" border="0" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://independent.academia.edu/DavidTalan">David Talan</a></div></div></ul></div><style type="text/css">.suggested-academics--header h3{font-size:16px;font-weight:500;line-height:20px}</style><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"></div></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Jose A Escario</h3></div><div class="js-work-strip profile--work_container" data-work-id="112332326"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/112332326/Simulaci%C3%B3n_virtual_e_lab_de_las_pr%C3%A1cticas_de_Fundamentos_y_T%C3%A9cnicas_Inmunol%C3%B3gicas"><img alt="Research paper thumbnail of Simulación virtual (e-lab) de las prácticas de Fundamentos y Técnicas Inmunológicas" class="work-thumbnail" src="https://attachments.academia-assets.com/109595130/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/112332326/Simulaci%C3%B3n_virtual_e_lab_de_las_pr%C3%A1cticas_de_Fundamentos_y_T%C3%A9cnicas_Inmunol%C3%B3gicas">Simulación virtual (e-lab) de las prácticas de Fundamentos y Técnicas Inmunológicas</a></div><div class="wp-workCard_item"><span>RELADA - Revista Electrónica de ADA-Madrid</span><span>, Dec 3, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Resumen: En el campo de la enseñanza de materias experimentales, el mayor coste de las clases prá...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Resumen: En el campo de la enseñanza de materias experimentales, el mayor coste de las clases prácticas, la necesidad de impartirlas en grupos más reducidos y la falta material de espacio y tiempo, ha provocado la pérdida de la coordinación teoría/practica y, consecuentemente, el concepto integral de la asignatura. Aprovechando el enorme desarrollo que han experimentado las aplicaciones multimedia y "applets", que conjugan la animación con interactividad y la aplicación de las nuevas técnicas, hemos querido efectuar un diseño interactivo en 3D, mediante el cual el discente pueda realizar las practicas reales de forma virtual. Se ha realizado, para la asignatura de Inmunología, la simulación de una práctica (Inmunofluorescencia) de forma completa en un laboratorio virtual. El discente, conociendo qué metodología debe aplicar, debe escoger tanto los reactivos como los materiales necesarios para llevar a cabo la práctica. Finalmente el alumno recibirá una puntuación en base a los errores cometidos durante su ejecución.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="434409b8e28cb0e93fd50c9c9f9e4d8f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109595130,"asset_id":112332326,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109595130/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="112332326"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="112332326"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 112332326; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=112332326]").text(description); $(".js-view-count[data-work-id=112332326]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 112332326; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='112332326']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "434409b8e28cb0e93fd50c9c9f9e4d8f" } } $('.js-work-strip[data-work-id=112332326]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":112332326,"title":"Simulación virtual (e-lab) de las prácticas de Fundamentos y Técnicas Inmunológicas","translated_title":"","metadata":{"grobid_abstract":"Resumen: En el campo de la enseñanza de materias experimentales, el mayor coste de las clases prácticas, la necesidad de impartirlas en grupos más reducidos y la falta material de espacio y tiempo, ha provocado la pérdida de la coordinación teoría/practica y, consecuentemente, el concepto integral de la asignatura. Aprovechando el enorme desarrollo que han experimentado las aplicaciones multimedia y \"applets\", que conjugan la animación con interactividad y la aplicación de las nuevas técnicas, hemos querido efectuar un diseño interactivo en 3D, mediante el cual el discente pueda realizar las practicas reales de forma virtual. Se ha realizado, para la asignatura de Inmunología, la simulación de una práctica (Inmunofluorescencia) de forma completa en un laboratorio virtual. El discente, conociendo qué metodología debe aplicar, debe escoger tanto los reactivos como los materiales necesarios para llevar a cabo la práctica. 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There was an increase in the stratified epithelium layers as well as edema produced by the increase of vascularization in the propia submucosa and infiltration of leukocytes. The proliferation of the vaginal epithelium favors the settlement and persistence of the parasitic infection. All of the findings corresponded with signs of a systemic disease being observed in the animals, including significant weight loss and also intestinal invasion. The entire inflammatory process has been corroborated by studies of adhesion molecules such as E-Selectin, VCAM-1 and PECAM-1. A correlation between the time of appearance and the perseverance of the inflammatory process with E-Selectin and VCAM-1 expression was observed, but not with PECAM-1. The strain with a higher pathogenicity was able to invade deep vaginal tissues and thus, parasites could not be detected by vaginal washings. This may be an important cause of diagnosis and treatment failure. Also, by the different localization of trichomonads, it appeared that the battle between host and parasite took place in different areas dependent upon the characteristics of the strain.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6c2d7cb883401661eaf017dfa6c355d2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077724,"asset_id":111580331,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077724/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111580331"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111580331"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111580331; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111580331]").text(description); $(".js-view-count[data-work-id=111580331]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111580331; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111580331']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6c2d7cb883401661eaf017dfa6c355d2" } } $('.js-work-strip[data-work-id=111580331]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111580331,"title":"Immunohistochemical study of the vaginal inflammatory response in experimental trichomoniasis","translated_title":"","metadata":{"publisher":"Elsevier BV","ai_title_tag":"Vaginal Inflammatory Response in Trichomoniasis","grobid_abstract":"In the present paper, the acute and subchronical inflammatory processes of the vaginal epithelial were studied in mice experimentally infected with two Trichomonas vaginalis strains of different pathogenicity, by means of histological and immunological methods. There was an increase in the stratified epithelium layers as well as edema produced by the increase of vascularization in the propia submucosa and infiltration of leukocytes. The proliferation of the vaginal epithelium favors the settlement and persistence of the parasitic infection. All of the findings corresponded with signs of a systemic disease being observed in the animals, including significant weight loss and also intestinal invasion. The entire inflammatory process has been corroborated by studies of adhesion molecules such as E-Selectin, VCAM-1 and PECAM-1. A correlation between the time of appearance and the perseverance of the inflammatory process with E-Selectin and VCAM-1 expression was observed, but not with PECAM-1. The strain with a higher pathogenicity was able to invade deep vaginal tissues and thus, parasites could not be detected by vaginal washings. This may be an important cause of diagnosis and treatment failure. Also, by the different localization of trichomonads, it appeared that the battle between host and parasite took place in different areas dependent upon the characteristics of the strain.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Acta Tropica","grobid_abstract_attachment_id":109077724},"translated_abstract":null,"internal_url":"https://www.academia.edu/111580331/Immunohistochemical_study_of_the_vaginal_inflammatory_response_in_experimental_trichomoniasis","translated_internal_url":"","created_at":"2023-12-16T10:56:42.965-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109077724,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077724/thumbnails/1.jpg","file_name":"j.actatropica.2009.12.00220231216-1-f4zm3k.pdf","download_url":"https://www.academia.edu/attachments/109077724/download_file","bulk_download_file_name":"Immunohistochemical_study_of_the_vaginal.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077724/j.actatropica.2009.12.00220231216-1-f4zm3k-libre.pdf?1702753247=\u0026response-content-disposition=attachment%3B+filename%3DImmunohistochemical_study_of_the_vaginal.pdf\u0026Expires=1743089736\u0026Signature=QX5b4Ky58XVY2vws2QsbEGLj603vQHPRipEd9wJ8rhMPVF8Pk68Y3-GFe9HdIW7sVusF9xQQySfFn-JFEd-uvl0LZb~o32jII2q7RQezIHxSgSMM5JaSBBGDw-mBn5LHLaIf5covVxE3pRtIsb6d0RfGKX-yQGtr1f4dCaEPXzV2rfFQGNzZvoqEt9MIFalU6yhnphe-H2dvP7j7sgnvmEpVU27ixhNGeE8nVtrMxs1w6U40tquQl055C9pVvoyPbEPurK743Hr4ZNpv9ZskSt6m2jxxvzBj6ds1-fPP3YCwn79gIZuLQ4R2nss9e9t0i~p0duh7tSNu7xGTaDbbBg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Immunohistochemical_study_of_the_vaginal_inflammatory_response_in_experimental_trichomoniasis","translated_slug":"","page_count":9,"language":"en","content_type":"Work","summary":"In the present paper, the acute and subchronical inflammatory processes of the vaginal epithelial were studied in mice experimentally infected with two Trichomonas vaginalis strains of different pathogenicity, by means of histological and immunological methods. There was an increase in the stratified epithelium layers as well as edema produced by the increase of vascularization in the propia submucosa and infiltration of leukocytes. The proliferation of the vaginal epithelium favors the settlement and persistence of the parasitic infection. All of the findings corresponded with signs of a systemic disease being observed in the animals, including significant weight loss and also intestinal invasion. The entire inflammatory process has been corroborated by studies of adhesion molecules such as E-Selectin, VCAM-1 and PECAM-1. A correlation between the time of appearance and the perseverance of the inflammatory process with E-Selectin and VCAM-1 expression was observed, but not with PECAM-1. The strain with a higher pathogenicity was able to invade deep vaginal tissues and thus, parasites could not be detected by vaginal washings. This may be an important cause of diagnosis and treatment failure. Also, by the different localization of trichomonads, it appeared that the battle between host and parasite took place in different areas dependent upon the characteristics of the strain.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109077724,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077724/thumbnails/1.jpg","file_name":"j.actatropica.2009.12.00220231216-1-f4zm3k.pdf","download_url":"https://www.academia.edu/attachments/109077724/download_file","bulk_download_file_name":"Immunohistochemical_study_of_the_vaginal.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077724/j.actatropica.2009.12.00220231216-1-f4zm3k-libre.pdf?1702753247=\u0026response-content-disposition=attachment%3B+filename%3DImmunohistochemical_study_of_the_vaginal.pdf\u0026Expires=1743089736\u0026Signature=QX5b4Ky58XVY2vws2QsbEGLj603vQHPRipEd9wJ8rhMPVF8Pk68Y3-GFe9HdIW7sVusF9xQQySfFn-JFEd-uvl0LZb~o32jII2q7RQezIHxSgSMM5JaSBBGDw-mBn5LHLaIf5covVxE3pRtIsb6d0RfGKX-yQGtr1f4dCaEPXzV2rfFQGNzZvoqEt9MIFalU6yhnphe-H2dvP7j7sgnvmEpVU27ixhNGeE8nVtrMxs1w6U40tquQl055C9pVvoyPbEPurK743Hr4ZNpv9ZskSt6m2jxxvzBj6ds1-fPP3YCwn79gIZuLQ4R2nss9e9t0i~p0duh7tSNu7xGTaDbbBg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation"},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":291136,"name":"Intestines","url":"https://www.academia.edu/Documents/in/Intestines"},{"id":376494,"name":"Epithelium","url":"https://www.academia.edu/Documents/in/Epithelium"},{"id":564878,"name":"Body Weight","url":"https://www.academia.edu/Documents/in/Body_Weight"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":1760295,"name":"Trichomoniasis","url":"https://www.academia.edu/Documents/in/Trichomoniasis"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":37207463,"url":"https://api.elsevier.com/content/article/PII:S0001706X09003738?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579215"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579215/Relationship_between_biological_behaviour_and_randomly_amplified_polymorphic_DNA_profiles_of_Trypanosoma_cruzi_strains"><img alt="Research paper thumbnail of Relationship between biological behaviour and randomly amplified polymorphic DNA profiles of Trypanosoma cruzi strains" class="work-thumbnail" src="https://attachments.academia-assets.com/109076960/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579215/Relationship_between_biological_behaviour_and_randomly_amplified_polymorphic_DNA_profiles_of_Trypanosoma_cruzi_strains">Relationship between biological behaviour and randomly amplified polymorphic DNA profiles of Trypanosoma cruzi strains</a></div><div class="wp-workCard_item"><span>Memorias Do Instituto Oswaldo Cruz</span><span>, Feb 1, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Once known some biological characteristics of six Trypanosoma cruzi strains, randomly amplified p...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Once known some biological characteristics of six Trypanosoma cruzi strains, randomly amplified polymorphic DNA (RAPD) analysis was made. Cluster analysis by UPGMA (unweighted pair group method analysis) was then applied both to biological parameters and RAPD profiles. Inspection of the UPGMA phenograms indicates identical clusters, so supporting that usefulness of biological parameters to characterization of T. cruzi strains still remains.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="73ba75b0797795d039bca5c542f6e985" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076960,"asset_id":111579215,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076960/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579215"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579215"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579215; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579215]").text(description); $(".js-view-count[data-work-id=111579215]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579215; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579215']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "73ba75b0797795d039bca5c542f6e985" } } $('.js-work-strip[data-work-id=111579215]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579215,"title":"Relationship between biological behaviour and randomly amplified polymorphic DNA profiles of Trypanosoma cruzi strains","translated_title":"","metadata":{"publisher":"Instituto Oswaldo Cruz, Ministério da Saúde","grobid_abstract":"Once known some biological characteristics of six Trypanosoma cruzi strains, randomly amplified polymorphic DNA (RAPD) analysis was made. Cluster analysis by UPGMA (unweighted pair group method analysis) was then applied both to biological parameters and RAPD profiles. Inspection of the UPGMA phenograms indicates identical clusters, so supporting that usefulness of biological parameters to characterization of T. cruzi strains still remains.","publication_date":{"day":1,"month":2,"year":2001,"errors":{}},"publication_name":"Memorias Do Instituto Oswaldo Cruz","grobid_abstract_attachment_id":109076960},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579215/Relationship_between_biological_behaviour_and_randomly_amplified_polymorphic_DNA_profiles_of_Trypanosoma_cruzi_strains","translated_internal_url":"","created_at":"2023-12-16T10:32:55.810-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109076960,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076960/thumbnails/1.jpg","file_name":"DM6HcJpKDQXr7n88436QQ3S.pdf","download_url":"https://www.academia.edu/attachments/109076960/download_file","bulk_download_file_name":"Relationship_between_biological_behaviou.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076960/DM6HcJpKDQXr7n88436QQ3S-libre.pdf?1702752322=\u0026response-content-disposition=attachment%3B+filename%3DRelationship_between_biological_behaviou.pdf\u0026Expires=1743089736\u0026Signature=gmIXipQaSuYVkpM-0GpUi~359z0pFu-4y3XVS8T6U67s0kx5nunnhq0PQBlD3KpoM18d5QdOaTdaTw1Pk9Eu4turbWWwedi~pvn6enmzQAePWbIyUW6bx6P1VDfaEdJ8FVOAv2hBXQH0sExAkug9iqAhFEcE2tUjJN8xT7foDWXLrT3f6m-J1Y5rJ98xBC3pMcfV6eTX7mVOvibHTCPQi0ksm3hBiITRotAeeTK0EMF1PteJbD1l2kYIh37aY6pOt2r1M8jsVYM810DoRwd23JMcaCk-Od5xJrDez8KQ2jrp7iySiftJreaXhChOEEDbq~MOj4N0dzsQGoGHlWe3DA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Relationship_between_biological_behaviour_and_randomly_amplified_polymorphic_DNA_profiles_of_Trypanosoma_cruzi_strains","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"Once known some biological characteristics of six Trypanosoma cruzi strains, randomly amplified polymorphic DNA (RAPD) analysis was made. Cluster analysis by UPGMA (unweighted pair group method analysis) was then applied both to biological parameters and RAPD profiles. Inspection of the UPGMA phenograms indicates identical clusters, so supporting that usefulness of biological parameters to characterization of T. cruzi strains still remains.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109076960,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076960/thumbnails/1.jpg","file_name":"DM6HcJpKDQXr7n88436QQ3S.pdf","download_url":"https://www.academia.edu/attachments/109076960/download_file","bulk_download_file_name":"Relationship_between_biological_behaviou.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076960/DM6HcJpKDQXr7n88436QQ3S-libre.pdf?1702752322=\u0026response-content-disposition=attachment%3B+filename%3DRelationship_between_biological_behaviou.pdf\u0026Expires=1743089736\u0026Signature=gmIXipQaSuYVkpM-0GpUi~359z0pFu-4y3XVS8T6U67s0kx5nunnhq0PQBlD3KpoM18d5QdOaTdaTw1Pk9Eu4turbWWwedi~pvn6enmzQAePWbIyUW6bx6P1VDfaEdJ8FVOAv2hBXQH0sExAkug9iqAhFEcE2tUjJN8xT7foDWXLrT3f6m-J1Y5rJ98xBC3pMcfV6eTX7mVOvibHTCPQi0ksm3hBiITRotAeeTK0EMF1PteJbD1l2kYIh37aY6pOt2r1M8jsVYM810DoRwd23JMcaCk-Od5xJrDez8KQ2jrp7iySiftJreaXhChOEEDbq~MOj4N0dzsQGoGHlWe3DA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":2749,"name":"Animal Behavior","url":"https://www.academia.edu/Documents/in/Animal_Behavior"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA"},{"id":153640,"name":"RAPD","url":"https://www.academia.edu/Documents/in/RAPD"},{"id":193456,"name":"UPGMA","url":"https://www.academia.edu/Documents/in/UPGMA"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":2547626,"name":"genetic heterogeneity","url":"https://www.academia.edu/Documents/in/genetic_heterogeneity"}],"urls":[{"id":37206621,"url":"https://www.scielo.br/j/mioc/a/DM6HcJpKDQXr7n88436QQ3S/?lang=en\u0026format=pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579212"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579212/Effect_of_piroxicam_metamizol_and_S_adenosylmethionine_in_a_murine_model_of_experimental_trichomoniasis"><img alt="Research paper thumbnail of Effect of piroxicam, metamizol, and S-adenosylmethionine in a murine model of experimental trichomoniasis" class="work-thumbnail" src="https://attachments.academia-assets.com/109077013/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579212/Effect_of_piroxicam_metamizol_and_S_adenosylmethionine_in_a_murine_model_of_experimental_trichomoniasis">Effect of piroxicam, metamizol, and S-adenosylmethionine in a murine model of experimental trichomoniasis</a></div><div class="wp-workCard_item"><span>Parasite</span><span>, Mar 1, 2005</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Biological effects of piroxicam, metamizol, and S-adenosylmethionine (S-AMET) have been tested in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Biological effects of piroxicam, metamizol, and S-adenosylmethionine (S-AMET) have been tested in NMRI mice infected intraperitoneally with Trichomonas vaginalis. An intraperitoneal treatment during ten preinfection days with piroxicam (10 mg/Kg/day), or metamizol (275 mg/Kg/day), but not with S-AMET (17 mg/Kg/day) induced a significant decrease of abdominal lesions and mortality, assessed by means of a pathogenicity index. The trichomonicidal activity of piroxicam, metamizol, and S-AMET was tested in vitro at the concentration of 300 µM, but found ineffective. These assays have shown the usefulness of the experimental trichomoniasis model for the study of the immunomodulating activity of synthetic drugs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e30b0d92aac1ccbcded10bb44b1edf8b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077013,"asset_id":111579212,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077013/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579212"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579212"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579212; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579212]").text(description); $(".js-view-count[data-work-id=111579212]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579212; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579212']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e30b0d92aac1ccbcded10bb44b1edf8b" } } $('.js-work-strip[data-work-id=111579212]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579212,"title":"Effect of piroxicam, metamizol, and S-adenosylmethionine in a murine model of experimental trichomoniasis","translated_title":"","metadata":{"publisher":"EDP Sciences","grobid_abstract":"Biological effects of piroxicam, metamizol, and S-adenosylmethionine (S-AMET) have been tested in NMRI mice infected intraperitoneally with Trichomonas vaginalis. 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Vero fibroblasts in 96-well tissue culture plates were infected with trypomastigote forms of T. cruzi. Amastigotes growth was estimated after 24 and 96 h both by microscopic counts of Giemsa-stained monolayers and enzyme-linked immunoIsorbent assay (ELISA). ELISA was performed directly on the fixed cultures using a rabbit anti-T. cruzi immunoIglobulin as the first antibody and a peroxidase-labelled antirabbit immunoglobulin as the second antibody. Three chemical series of structural analogous of gentian violet, thiadiazines and derivatives of 5-nitrothiophene-2-carbaldehyde as well as three reference compounds (nifurtimox, benznidazole and gentian violet) were then assayed. The anti-T. cruzi activity of all of them had been determined previously by microscopic counting of Giemsa-stained infected cultures. None of the assayed compounds showed better activity than the reference ones, but the application of the enzyme immunoassay to quantify the inhibition of growth amastigotes is of great interest, as it yielded results comparable with microscopic counts. ELISA can be applied to pharmacological screening, with some advantages over the microscopic examination, including possible automation, rapidity and objectivity in assessment.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579209"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579209"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579209; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579209]").text(description); $(".js-view-count[data-work-id=111579209]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579209; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579209']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=111579209]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579209,"title":"Evaluation of drug activity against intracellular forms of Trypanosoma cruzi employing enzyme immunoassay","translated_title":"","metadata":{"abstract":"To describe application of a new method for the evaluation of anti-Trypanosoma cruzi activity against intracellular forms. Vero fibroblasts in 96-well tissue culture plates were infected with trypomastigote forms of T. cruzi. Amastigotes growth was estimated after 24 and 96 h both by microscopic counts of Giemsa-stained monolayers and enzyme-linked immunoIsorbent assay (ELISA). ELISA was performed directly on the fixed cultures using a rabbit anti-T. cruzi immunoIglobulin as the first antibody and a peroxidase-labelled antirabbit immunoglobulin as the second antibody. Three chemical series of structural analogous of gentian violet, thiadiazines and derivatives of 5-nitrothiophene-2-carbaldehyde as well as three reference compounds (nifurtimox, benznidazole and gentian violet) were then assayed. The anti-T. cruzi activity of all of them had been determined previously by microscopic counting of Giemsa-stained infected cultures. None of the assayed compounds showed better activity than the reference ones, but the application of the enzyme immunoassay to quantify the inhibition of growth amastigotes is of great interest, as it yielded results comparable with microscopic counts. ELISA can be applied to pharmacological screening, with some advantages over the microscopic examination, including possible automation, rapidity and objectivity in assessment.","publisher":"Wiley-Blackwell","publication_date":{"day":null,"month":null,"year":2000,"errors":{}},"publication_name":"Journal of Clinical Pharmacy and Therapeutics"},"translated_abstract":"To describe application of a new method for the evaluation of anti-Trypanosoma cruzi activity against intracellular forms. Vero fibroblasts in 96-well tissue culture plates were infected with trypomastigote forms of T. cruzi. 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ELISA can be applied to pharmacological screening, with some advantages over the microscopic examination, including possible automation, rapidity and objectivity in assessment.","internal_url":"https://www.academia.edu/111579209/Evaluation_of_drug_activity_against_intracellular_forms_of_Trypanosoma_cruzi_employing_enzyme_immunoassay","translated_internal_url":"","created_at":"2023-12-16T10:32:54.471-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Evaluation_of_drug_activity_against_intracellular_forms_of_Trypanosoma_cruzi_employing_enzyme_immunoassay","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"To describe application of a new method for the evaluation of anti-Trypanosoma cruzi activity against intracellular forms. Vero fibroblasts in 96-well tissue culture plates were infected with trypomastigote forms of T. cruzi. Amastigotes growth was estimated after 24 and 96 h both by microscopic counts of Giemsa-stained monolayers and enzyme-linked immunoIsorbent assay (ELISA). ELISA was performed directly on the fixed cultures using a rabbit anti-T. cruzi immunoIglobulin as the first antibody and a peroxidase-labelled antirabbit immunoglobulin as the second antibody. Three chemical series of structural analogous of gentian violet, thiadiazines and derivatives of 5-nitrothiophene-2-carbaldehyde as well as three reference compounds (nifurtimox, benznidazole and gentian violet) were then assayed. The anti-T. cruzi activity of all of them had been determined previously by microscopic counting of Giemsa-stained infected cultures. None of the assayed compounds showed better activity than the reference ones, but the application of the enzyme immunoassay to quantify the inhibition of growth amastigotes is of great interest, as it yielded results comparable with microscopic counts. ELISA can be applied to pharmacological screening, with some advantages over the microscopic examination, including possible automation, rapidity and objectivity in assessment.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":99708,"name":"Clinical","url":"https://www.academia.edu/Documents/in/Clinical"},{"id":111012,"name":"Plant tissue Culture Techniques","url":"https://www.academia.edu/Documents/in/Plant_tissue_Culture_Techniques"},{"id":225340,"name":"Chagas disease","url":"https://www.academia.edu/Documents/in/Chagas_disease"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":511535,"name":"Immunoassay","url":"https://www.academia.edu/Documents/in/Immunoassay"},{"id":619383,"name":"Benznidazole","url":"https://www.academia.edu/Documents/in/Benznidazole"},{"id":619386,"name":"Nifurtimox","url":"https://www.academia.edu/Documents/in/Nifurtimox"},{"id":744836,"name":"Trypanocidal Agents","url":"https://www.academia.edu/Documents/in/Trypanocidal_Agents"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1312086,"name":"Immunoglobulins","url":"https://www.academia.edu/Documents/in/Immunoglobulins"},{"id":2032101,"name":"Intracellular","url":"https://www.academia.edu/Documents/in/Intracellular"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"},{"id":3948095,"name":" Giemsa stain","url":"https://www.academia.edu/Documents/in/Giemsa_stain"}],"urls":[{"id":37206615,"url":"https://doi.org/10.1046/j.1365-2710.2000.00256.x"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579207"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579207/A_new_pharmacological_screening_assay_with_Trypanosoma_cruzi_epimastigotes_expressing_galactosidase"><img alt="Research paper thumbnail of A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing ?-galactosidase" class="work-thumbnail" src="https://attachments.academia-assets.com/109077011/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579207/A_new_pharmacological_screening_assay_with_Trypanosoma_cruzi_epimastigotes_expressing_galactosidase">A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing ?-galactosidase</a></div><div class="wp-workCard_item"><span>Parasitology Research</span><span>, Jan 29, 2005</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (c...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic b-galactosidase released into the culture on membrane lysis in the presence of the substrate chlorophenol red b-D-galactopyranoside (CPRG). The experimental conditions were adjusted to find those in which the relationship between epimastigote number and CPRG absorbance was linear over the widest possible range. Absorbance was significantly correlated with the number epimastigote from 5•10 3 to 1.2•10 6 parasites/ml (r=0.98, P<0.01). The optimal final concentration of CPRG was 200 lM and the optimal incubation period was 6 h when parasites were incubated for 3 days. Once the assay was standardized, the trypanocidal activities of nifurtimox and benznidazole were determined both by CPRG assay and microscopic counting, demonstrating the methods utility for drugscreening. The efficacy obtained was comparable to that obtained with the manual method.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fd5a683fb435cef90567f0b96dc1d92e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077011,"asset_id":111579207,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077011/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579207"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579207"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579207; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579207]").text(description); $(".js-view-count[data-work-id=111579207]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579207; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579207']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fd5a683fb435cef90567f0b96dc1d92e" } } $('.js-work-strip[data-work-id=111579207]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579207,"title":"A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing ?-galactosidase","translated_title":"","metadata":{"publisher":"Springer Science+Business Media","grobid_abstract":"We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic b-galactosidase released into the culture on membrane lysis in the presence of the substrate chlorophenol red b-D-galactopyranoside (CPRG). The experimental conditions were adjusted to find those in which the relationship between epimastigote number and CPRG absorbance was linear over the widest possible range. Absorbance was significantly correlated with the number epimastigote from 5•10 3 to 1.2•10 6 parasites/ml (r=0.98, P\u003c0.01). The optimal final concentration of CPRG was 200 lM and the optimal incubation period was 6 h when parasites were incubated for 3 days. Once the assay was standardized, the trypanocidal activities of nifurtimox and benznidazole were determined both by CPRG assay and microscopic counting, demonstrating the methods utility for drugscreening. The efficacy obtained was comparable to that obtained with the manual method.","publication_date":{"day":29,"month":1,"year":2005,"errors":{}},"publication_name":"Parasitology Research","grobid_abstract_attachment_id":109077011},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579207/A_new_pharmacological_screening_assay_with_Trypanosoma_cruzi_epimastigotes_expressing_galactosidase","translated_internal_url":"","created_at":"2023-12-16T10:32:53.973-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109077011,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077011/thumbnails/1.jpg","file_name":"s00436-005-1300-320231216-1-awp2zu.pdf","download_url":"https://www.academia.edu/attachments/109077011/download_file","bulk_download_file_name":"A_new_pharmacological_screening_assay_wi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077011/s00436-005-1300-320231216-1-awp2zu-libre.pdf?1702752314=\u0026response-content-disposition=attachment%3B+filename%3DA_new_pharmacological_screening_assay_wi.pdf\u0026Expires=1743089736\u0026Signature=VPQ72d5ijJJXn9nT8nbYuKV2Gk1lEURc3iw2DvPpb5WjY7o14aGrEKFBABSv4jzX~KZEDvZTpxjCMfdIB1lqwnVYgnqUi5MOYUV92fq5LoBd35czcKH4u2p3lvf~gEKpsWdsoTAS6WHTw5djYkCnDbZS0JMwyJluzl~6nTGziMk4DWTd6mK1QLoyZtIhA8iFKF3YVfq5FM-XXmXTGKmTs8g67v-C40XYJJSaEebMtp474229YMpEn3nmRXeBbpzb-5TaoYBaBzJ6mdVBVQdZ1tmXTYmB1OaQAU-oqw1jVT5vQdVUZLl9AnCycwEZP5t8JM3zaTRvh1JHQqDGCsSDtw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_new_pharmacological_screening_assay_with_Trypanosoma_cruzi_epimastigotes_expressing_galactosidase","translated_slug":"","page_count":3,"language":"en","content_type":"Work","summary":"We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic b-galactosidase released into the culture on membrane lysis in the presence of the substrate chlorophenol red b-D-galactopyranoside (CPRG). The experimental conditions were adjusted to find those in which the relationship between epimastigote number and CPRG absorbance was linear over the widest possible range. Absorbance was significantly correlated with the number epimastigote from 5•10 3 to 1.2•10 6 parasites/ml (r=0.98, P\u003c0.01). The optimal final concentration of CPRG was 200 lM and the optimal incubation period was 6 h when parasites were incubated for 3 days. Once the assay was standardized, the trypanocidal activities of nifurtimox and benznidazole were determined both by CPRG assay and microscopic counting, demonstrating the methods utility for drugscreening. The efficacy obtained was comparable to that obtained with the manual method.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109077011,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077011/thumbnails/1.jpg","file_name":"s00436-005-1300-320231216-1-awp2zu.pdf","download_url":"https://www.academia.edu/attachments/109077011/download_file","bulk_download_file_name":"A_new_pharmacological_screening_assay_wi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077011/s00436-005-1300-320231216-1-awp2zu-libre.pdf?1702752314=\u0026response-content-disposition=attachment%3B+filename%3DA_new_pharmacological_screening_assay_wi.pdf\u0026Expires=1743089736\u0026Signature=VPQ72d5ijJJXn9nT8nbYuKV2Gk1lEURc3iw2DvPpb5WjY7o14aGrEKFBABSv4jzX~KZEDvZTpxjCMfdIB1lqwnVYgnqUi5MOYUV92fq5LoBd35czcKH4u2p3lvf~gEKpsWdsoTAS6WHTw5djYkCnDbZS0JMwyJluzl~6nTGziMk4DWTd6mK1QLoyZtIhA8iFKF3YVfq5FM-XXmXTGKmTs8g67v-C40XYJJSaEebMtp474229YMpEn3nmRXeBbpzb-5TaoYBaBzJ6mdVBVQdZ1tmXTYmB1OaQAU-oqw1jVT5vQdVUZLl9AnCycwEZP5t8JM3zaTRvh1JHQqDGCsSDtw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":164,"name":"Parasitology","url":"https://www.academia.edu/Documents/in/Parasitology"},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli"},{"id":122642,"name":"Drug Screening","url":"https://www.academia.edu/Documents/in/Drug_Screening"},{"id":178351,"name":"Spectrophotometry","url":"https://www.academia.edu/Documents/in/Spectrophotometry"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":619383,"name":"Benznidazole","url":"https://www.academia.edu/Documents/in/Benznidazole"},{"id":619386,"name":"Nifurtimox","url":"https://www.academia.edu/Documents/in/Nifurtimox"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":744836,"name":"Trypanocidal Agents","url":"https://www.academia.edu/Documents/in/Trypanocidal_Agents"},{"id":3557974,"name":"Chlorophenols","url":"https://www.academia.edu/Documents/in/Chlorophenols"}],"urls":[{"id":37206613,"url":"https://doi.org/10.1007/s00436-005-1300-3"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579205"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579205/Biological_characterization_of_Trypanosoma_cruzi_strains"><img alt="Research paper thumbnail of Biological characterization of Trypanosoma cruzi strains" class="work-thumbnail" src="https://attachments.academia-assets.com/109076954/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579205/Biological_characterization_of_Trypanosoma_cruzi_strains">Biological characterization of Trypanosoma cruzi strains</a></div><div class="wp-workCard_item"><span>Memorias Do Instituto Oswaldo Cruz</span><span>, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Biological parameters of five Trypanosoma cruzi strains from different sources were determined in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Biological parameters of five Trypanosoma cruzi strains from different sources were determined in order to know the laboratory behaviour of natural populations. The parameters evaluated were growth kinetics of epimastigotes, differentiation into metacyclic forms, infectivity in mammalian cells grown in vitro and parasite susceptibility to nifurtimox, benznidazole and gentian violet. Differences in transformation to metacyclic, in the percentage of infected cells as well as in the number of amastigotes per cell were observed among the strains. Regarding to pharmacological assays, Y strain was the most sensitive to the three assayed compounds. These data demonstrate the heterogeneity of natural populations of T. cruzi, the only responsible of infection in humans.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="93866e5b4cf72233ab5d29b1097fb71f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076954,"asset_id":111579205,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076954/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579205"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579205"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579205; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579205]").text(description); $(".js-view-count[data-work-id=111579205]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579205; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579205']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "93866e5b4cf72233ab5d29b1097fb71f" } } $('.js-work-strip[data-work-id=111579205]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579205,"title":"Biological characterization of Trypanosoma cruzi strains","translated_title":"","metadata":{"publisher":"Instituto Oswaldo Cruz, Ministério da Saúde","grobid_abstract":"Biological parameters of five Trypanosoma cruzi strains from different sources were determined in order to know the laboratory behaviour of natural populations. The parameters evaluated were growth kinetics of epimastigotes, differentiation into metacyclic forms, infectivity in mammalian cells grown in vitro and parasite susceptibility to nifurtimox, benznidazole and gentian violet. Differences in transformation to metacyclic, in the percentage of infected cells as well as in the number of amastigotes per cell were observed among the strains. Regarding to pharmacological assays, Y strain was the most sensitive to the three assayed compounds. These data demonstrate the heterogeneity of natural populations of T. cruzi, the only responsible of infection in humans.","publication_date":{"day":null,"month":null,"year":2001,"errors":{}},"publication_name":"Memorias Do Instituto Oswaldo Cruz","grobid_abstract_attachment_id":109076954},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579205/Biological_characterization_of_Trypanosoma_cruzi_strains","translated_internal_url":"","created_at":"2023-12-16T10:32:53.498-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109076954,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076954/thumbnails/1.jpg","file_name":"QtSc4VkpHG696KnDDL9GWPt.pdf","download_url":"https://www.academia.edu/attachments/109076954/download_file","bulk_download_file_name":"Biological_characterization_of_Trypanoso.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076954/QtSc4VkpHG696KnDDL9GWPt-libre.pdf?1702752322=\u0026response-content-disposition=attachment%3B+filename%3DBiological_characterization_of_Trypanoso.pdf\u0026Expires=1743089736\u0026Signature=G97YiLNGF4uPGzAeqllWKWdvEx4Hec7gUbjqcHgXbuk9dqOnx2HGZEXZCoOld4PmGhwKXm~Uun65qVHma4AXZBYq8YWv6BLExcAyOw6JhcKefAB6bRt3rChBAjA0daHQoyLY3~ZiWejaKlXkDJsrTDPTPzgrGUsXUOZqADuz32x8Zcn-EIK6g-iB6pLngyCxnprhNuN1dNVFdsR9oTfXiLCErQcsIYuA2O7vhSSJ5-Zl0t1fjKh-yCyrv0hllUbMPkstfTu3sIKultNBEhQQrz5yBqa26BMTpGAQ2QkCWqH0MoDRHWNeA3jgIK9gpQu~Nf1GHrf6Kg5J75n2aymLeg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Biological_characterization_of_Trypanosoma_cruzi_strains","translated_slug":"","page_count":7,"language":"en","content_type":"Work","summary":"Biological parameters of five Trypanosoma cruzi strains from different sources were determined in order to know the laboratory behaviour of natural populations. The parameters evaluated were growth kinetics of epimastigotes, differentiation into metacyclic forms, infectivity in mammalian cells grown in vitro and parasite susceptibility to nifurtimox, benznidazole and gentian violet. Differences in transformation to metacyclic, in the percentage of infected cells as well as in the number of amastigotes per cell were observed among the strains. Regarding to pharmacological assays, Y strain was the most sensitive to the three assayed compounds. These data demonstrate the heterogeneity of natural populations of T. cruzi, the only responsible of infection in humans.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109076954,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076954/thumbnails/1.jpg","file_name":"QtSc4VkpHG696KnDDL9GWPt.pdf","download_url":"https://www.academia.edu/attachments/109076954/download_file","bulk_download_file_name":"Biological_characterization_of_Trypanoso.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076954/QtSc4VkpHG696KnDDL9GWPt-libre.pdf?1702752322=\u0026response-content-disposition=attachment%3B+filename%3DBiological_characterization_of_Trypanoso.pdf\u0026Expires=1743089736\u0026Signature=G97YiLNGF4uPGzAeqllWKWdvEx4Hec7gUbjqcHgXbuk9dqOnx2HGZEXZCoOld4PmGhwKXm~Uun65qVHma4AXZBYq8YWv6BLExcAyOw6JhcKefAB6bRt3rChBAjA0daHQoyLY3~ZiWejaKlXkDJsrTDPTPzgrGUsXUOZqADuz32x8Zcn-EIK6g-iB6pLngyCxnprhNuN1dNVFdsR9oTfXiLCErQcsIYuA2O7vhSSJ5-Zl0t1fjKh-yCyrv0hllUbMPkstfTu3sIKultNBEhQQrz5yBqa26BMTpGAQ2QkCWqH0MoDRHWNeA3jgIK9gpQu~Nf1GHrf6Kg5J75n2aymLeg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":464116,"name":"Repetitive Strain Injury","url":"https://www.academia.edu/Documents/in/Repetitive_Strain_Injury"},{"id":619383,"name":"Benznidazole","url":"https://www.academia.edu/Documents/in/Benznidazole"},{"id":619386,"name":"Nifurtimox","url":"https://www.academia.edu/Documents/in/Nifurtimox"},{"id":2420252,"name":"Infectivity","url":"https://www.academia.edu/Documents/in/Infectivity"}],"urls":[{"id":37206612,"url":"https://www.scielo.br/j/mioc/a/QtSc4VkpHG696KnDDL9GWPt/?lang=en\u0026format=pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579200"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579200/Effect_of_Anapsos_in_a_murine_model_of_experimental_trichomoniasis"><img alt="Research paper thumbnail of Effect of Anapsos® in a murine model of experimental trichomoniasis" class="work-thumbnail" src="https://attachments.academia-assets.com/109077027/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579200/Effect_of_Anapsos_in_a_murine_model_of_experimental_trichomoniasis">Effect of Anapsos® in a murine model of experimental trichomoniasis</a></div><div class="wp-workCard_item"><span>Parasite</span><span>, Dec 1, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Immunomodulator effect of Anapsos® (Polypodium leucotomos extract) in NMRI (US Naval Medical Rese...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Immunomodulator effect of Anapsos® (Polypodium leucotomos extract) in NMRI (US Naval Medical Research Institute) outbred mice infected by the intraperitoneal route with 10 7 Trichomonas vaginalis has been tested. Gross histopathologic changes in abdominal organs and mortality rate, as a consequence of the pathogenicity of the protozoa and the immune response of the host, were evaluated. Among the different treatment regimes assayed, Anapsos® at doses of 20 mg/Kg/day administered for 1 0 days before infection decreases the parasite pathogenicity index (PI) in the treated animals when compared to those of the untreated control group. The immunosuppresor treatments with azathioprine (100 mg/Kg/day x 1), cyclophosphamide (100 mg/Kg/day x 1), and FK-506 (10 mg/Kg/day x 10) significantly decreased the PI, while an immunostimulant treatment with glycophosphopeptical (13 mg/Kg/day x 10) increased it. These assays have shown the usefulness of the murine model of experimental trichomoniasis for the study of immunomodulator activity of natural or synthetic drugs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f0e5fdcf7e2c3d5be8a231ebe4a98cec" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077027,"asset_id":111579200,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077027/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579200"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579200"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579200; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579200]").text(description); $(".js-view-count[data-work-id=111579200]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579200; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579200']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f0e5fdcf7e2c3d5be8a231ebe4a98cec" } } $('.js-work-strip[data-work-id=111579200]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579200,"title":"Effect of Anapsos® in a murine model of experimental trichomoniasis","translated_title":"","metadata":{"publisher":"EDP Sciences","grobid_abstract":"Immunomodulator effect of Anapsos® (Polypodium leucotomos extract) in NMRI (US Naval Medical Research Institute) outbred mice infected by the intraperitoneal route with 10 7 Trichomonas vaginalis has been tested. Gross histopathologic changes in abdominal organs and mortality rate, as a consequence of the pathogenicity of the protozoa and the immune response of the host, were evaluated. Among the different treatment regimes assayed, Anapsos® at doses of 20 mg/Kg/day administered for 1 0 days before infection decreases the parasite pathogenicity index (PI) in the treated animals when compared to those of the untreated control group. The immunosuppresor treatments with azathioprine (100 mg/Kg/day x 1), cyclophosphamide (100 mg/Kg/day x 1), and FK-506 (10 mg/Kg/day x 10) significantly decreased the PI, while an immunostimulant treatment with glycophosphopeptical (13 mg/Kg/day x 10) increased it. These assays have shown the usefulness of the murine model of experimental trichomoniasis for the study of immunomodulator activity of natural or synthetic drugs.","publication_date":{"day":1,"month":12,"year":2003,"errors":{}},"publication_name":"Parasite","grobid_abstract_attachment_id":109077027},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579200/Effect_of_Anapsos_in_a_murine_model_of_experimental_trichomoniasis","translated_internal_url":"","created_at":"2023-12-16T10:32:52.607-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109077027,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077027/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/109077027/download_file","bulk_download_file_name":"Effect_of_Anapsos_in_a_murine_model_of_e.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077027/pdf-libre.pdf?1702752323=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_Anapsos_in_a_murine_model_of_e.pdf\u0026Expires=1743089736\u0026Signature=FFPCKs6yJKwAqrIzmxBVUvBnQDDkCZ0Y81QqZyLIB7V85FwZpHSWaHtUGSEZEpAH-MCu0919W0MeFtcbuTrQfepVH53hLJg~JTWK8fUyfW2HAApl6yNm0OYVJnhYMRSKFlsHYWyaFQ1J1Hh7FgxVECygo-Z8Lmtk8XaFn20a~0yv2CtpwLTUkHmuA6~7f8vr2hhHkkXV-UT6ZgCWGgruxeHUUiBYr-tFMKmA8PRQxdrSIFYXDb-KNvYT27J0mSdT4fAjdF8WRFitbBJpBnRvf0CoI7UXXlksEfm0v9uDnARs2tDXDiTOswT1x0rJSehzxfEQFkLyHGEboKwUElNgaQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Effect_of_Anapsos_in_a_murine_model_of_experimental_trichomoniasis","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"Immunomodulator effect of Anapsos® (Polypodium leucotomos extract) in NMRI (US Naval Medical Research Institute) outbred mice infected by the intraperitoneal route with 10 7 Trichomonas vaginalis has been tested. Gross histopathologic changes in abdominal organs and mortality rate, as a consequence of the pathogenicity of the protozoa and the immune response of the host, were evaluated. Among the different treatment regimes assayed, Anapsos® at doses of 20 mg/Kg/day administered for 1 0 days before infection decreases the parasite pathogenicity index (PI) in the treated animals when compared to those of the untreated control group. The immunosuppresor treatments with azathioprine (100 mg/Kg/day x 1), cyclophosphamide (100 mg/Kg/day x 1), and FK-506 (10 mg/Kg/day x 10) significantly decreased the PI, while an immunostimulant treatment with glycophosphopeptical (13 mg/Kg/day x 10) increased it. These assays have shown the usefulness of the murine model of experimental trichomoniasis for the study of immunomodulator activity of natural or synthetic drugs.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109077027,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077027/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/109077027/download_file","bulk_download_file_name":"Effect_of_Anapsos_in_a_murine_model_of_e.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077027/pdf-libre.pdf?1702752323=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_Anapsos_in_a_murine_model_of_e.pdf\u0026Expires=1743089736\u0026Signature=FFPCKs6yJKwAqrIzmxBVUvBnQDDkCZ0Y81QqZyLIB7V85FwZpHSWaHtUGSEZEpAH-MCu0919W0MeFtcbuTrQfepVH53hLJg~JTWK8fUyfW2HAApl6yNm0OYVJnhYMRSKFlsHYWyaFQ1J1Hh7FgxVECygo-Z8Lmtk8XaFn20a~0yv2CtpwLTUkHmuA6~7f8vr2hhHkkXV-UT6ZgCWGgruxeHUUiBYr-tFMKmA8PRQxdrSIFYXDb-KNvYT27J0mSdT4fAjdF8WRFitbBJpBnRvf0CoI7UXXlksEfm0v9uDnARs2tDXDiTOswT1x0rJSehzxfEQFkLyHGEboKwUElNgaQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":29974,"name":"Disease susceptibility","url":"https://www.academia.edu/Documents/in/Disease_susceptibility"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":94271,"name":"Parasite","url":"https://www.academia.edu/Documents/in/Parasite"},{"id":143830,"name":"Immunomodulation","url":"https://www.academia.edu/Documents/in/Immunomodulation"},{"id":324154,"name":"Immune system","url":"https://www.academia.edu/Documents/in/Immune_system"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts"},{"id":770335,"name":"Immunostimulant","url":"https://www.academia.edu/Documents/in/Immunostimulant"},{"id":1198586,"name":"FK","url":"https://www.academia.edu/Documents/in/FK"},{"id":1198588,"name":"Cyclophosphamide","url":"https://www.academia.edu/Documents/in/Cyclophosphamide"},{"id":1533653,"name":"Glycosides","url":"https://www.academia.edu/Documents/in/Glycosides"},{"id":1740531,"name":"Azathioprine","url":"https://www.academia.edu/Documents/in/Azathioprine"},{"id":2463495,"name":"Random Allocation","url":"https://www.academia.edu/Documents/in/Random_Allocation"},{"id":2562571,"name":"Immunosuppressive Agents","url":"https://www.academia.edu/Documents/in/Immunosuppressive_Agents"}],"urls":[{"id":37206608,"url":"https://doi.org/10.1051/parasite/2003104303"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579199"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579199/Evaluation_of_a_murine_model_of_experimental_trichomoniasis"><img alt="Research paper thumbnail of Evaluation of a murine model of experimental trichomoniasis" class="work-thumbnail" src="https://attachments.academia-assets.com/109077024/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579199/Evaluation_of_a_murine_model_of_experimental_trichomoniasis">Evaluation of a murine model of experimental trichomoniasis</a></div><div class="wp-workCard_item"><span>Parasite</span><span>, Jun 1, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">By using a reference strain of Trichomonas vaginalis and the intraperitoneal route for infecting ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">By using a reference strain of Trichomonas vaginalis and the intraperitoneal route for infecting animals, the influence of the strain of mice, the time observation and the inoculation doses were followed in order to standardize the optimal conditions for the evolution of experimental trichomoniasis. Our tesults suggest that the inoculation of BALB/c mice with 10 7 trichomonads and the semiquantitative assessment at day 15 postinfection of the grosspathologic changes in the abdominal cavity-peritoneum, spleen, pancreas, stomach and liver-as well as the presence of ascitic fluid and mortality, maybe a suitable laboratory model of trichomoniasis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="74f6559607afe689f9759207c788e73d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077024,"asset_id":111579199,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077024/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579199"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579199"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579199; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579199]").text(description); $(".js-view-count[data-work-id=111579199]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579199; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579199']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "74f6559607afe689f9759207c788e73d" } } $('.js-work-strip[data-work-id=111579199]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579199,"title":"Evaluation of a murine model of experimental trichomoniasis","translated_title":"","metadata":{"publisher":"EDP Sciences","grobid_abstract":"By using a reference strain of Trichomonas vaginalis and the intraperitoneal route for infecting animals, the influence of the strain of mice, the time observation and the inoculation doses were followed in order to standardize the optimal conditions for the evolution of experimental trichomoniasis. 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A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cce8d8faa4df75bde894412749ada07b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076951,"asset_id":111579196,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076951/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579196"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579196"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579196; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579196]").text(description); $(".js-view-count[data-work-id=111579196]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579196; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579196']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cce8d8faa4df75bde894412749ada07b" } } $('.js-work-strip[data-work-id=111579196]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579196,"title":"Biological variability in clinical isolates of Trichomonas vaginalis","translated_title":"","metadata":{"publisher":"Instituto Oswaldo Cruz, Ministério da Saúde","grobid_abstract":"Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Goverment of Madrid. 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Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.","publication_date":{"day":1,"month":9,"year":2002,"errors":{}},"publication_name":"Memorias Do Instituto Oswaldo Cruz","grobid_abstract_attachment_id":109076951},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579196/Biological_variability_in_clinical_isolates_of_Trichomonas_vaginalis","translated_internal_url":"","created_at":"2023-12-16T10:32:51.582-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109076951,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076951/thumbnails/1.jpg","file_name":"FVHzmz6DDF3BXH9cjyQSyhD.pdf","download_url":"https://www.academia.edu/attachments/109076951/download_file","bulk_download_file_name":"Biological_variability_in_clinical_isola.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076951/FVHzmz6DDF3BXH9cjyQSyhD-libre.pdf?1702752319=\u0026response-content-disposition=attachment%3B+filename%3DBiological_variability_in_clinical_isola.pdf\u0026Expires=1743089736\u0026Signature=CSuSQmSYFIa0hNDWDG9OH4jJdh0OrClgqMwzVSzA~dUsyJ5lHtEW6lRZcNJScDvjDv42bYhxKWpLtfj9q3QnT59gb3P5FM-Nk9aGzcy~tLHuSNuXKT7PAFlc2eH3198Qe3IMOglgaXLJTtwQBMr~bUVMviNmqZyMIYpnmrRn3O5kVt7-axFsxhhH99v2WCIpTOoqY288R9j5V5NtRUuyCNOZ5PF9prUYCRelfL6vypgQkFywOKtfNBm5-3kNXooWx4b6eYZ~4EK~uMlRLBgFxxuTnrMIyR8BaSmHL7HawVfGhLB3aN6H2uQJYJeP-OAbNJZEKrVrinIsf7ouojuVwA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Biological_variability_in_clinical_isolates_of_Trichomonas_vaginalis","translated_slug":"","page_count":4,"language":"en","content_type":"Work","summary":"Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Goverment of Madrid. A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109076951,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076951/thumbnails/1.jpg","file_name":"FVHzmz6DDF3BXH9cjyQSyhD.pdf","download_url":"https://www.academia.edu/attachments/109076951/download_file","bulk_download_file_name":"Biological_variability_in_clinical_isola.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076951/FVHzmz6DDF3BXH9cjyQSyhD-libre.pdf?1702752319=\u0026response-content-disposition=attachment%3B+filename%3DBiological_variability_in_clinical_isola.pdf\u0026Expires=1743089737\u0026Signature=TyWvujzaGo~yiy9mmL-l9K6TIA4bW2NJ5toXZfjTxMtmxpExA1ySfN5yZPQmYQiV~P0wIs1PfMvkUnp-7p7~43cwVCY~4Cx3YIWw6fFak27Mckn4g~CYfVTfd-6nL2lLkfpeyv-SWty-p-wh5HllgftBhILqjx2SbSU8plv1atc4tjRvb3sKN0YWXbqmRO9xYzunbPyZRliIXOGXZOvuezxpepfe7GcIAZDv3sokD3soHkH5KgIrrKBQ~kTGC~gNvPZbTmVKkbilVcUzgj6st5ow9Eej9rOUvFyYzkFAZNgNrfmSRKIsEJQbMiKMyVOucla8aX8TYSSd9yntcSMECg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":52873,"name":"Virulence","url":"https://www.academia.edu/Documents/in/Virulence"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":413804,"name":"Inoculation","url":"https://www.academia.edu/Documents/in/Inoculation"},{"id":957793,"name":"Pathogenicity","url":"https://www.academia.edu/Documents/in/Pathogenicity"},{"id":1303572,"name":"Metronidazole","url":"https://www.academia.edu/Documents/in/Metronidazole"},{"id":4063964,"name":"Trichomonas","url":"https://www.academia.edu/Documents/in/Trichomonas"}],"urls":[{"id":37206605,"url":"https://www.scielo.br/j/mioc/a/FVHzmz6DDF3BXH9cjyQSyhD/?lang=en\u0026format=pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579194"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/111579194/Activity_Assays_of_Thiadiazine_Derivatives_on_and_lt_i_and_gt_Trichomonas_vaginalis_and_lt_i_and_gt_and_Amastigote_Forms_of_and_lt_i_and_gt_Trypanosoma_cruzi_and_lt_i_and_gt"><img alt="Research paper thumbnail of Activity Assays of Thiadiazine Derivatives on &lt;i&gt;Trichomonas vaginalis &lt;/i&gt;and Amastigote Forms of &lt;i&gt;Trypanosoma cruzi&lt;/i&gt" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Activity Assays of Thiadiazine Derivatives on &lt;i&gt;Trichomonas vaginalis &lt;/i&gt;and Amastigote Forms of &lt;i&gt;Trypanosoma cruzi&lt;/i&gt</div><div class="wp-workCard_item"><span>Chemotherapy</span><span>, 1992</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. In vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. The capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. One of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronidazole, although higher doses were required. Its toxicity on cell proliferation in tissue culture was moderate. In vitro assays on amastigote forms of Trypanosoma cruzi were carried out using cultures of Vero cells infected with metacyclic trypomastigotes. For one compound (1) trypanocidal activity resembled that of nifurtimox as assessed by microscopic counts of infected and uninfected cells. Unfortunately, this compound showed a high degree of cytotoxicity on Vero cell cultures.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579194"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579194"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579194; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579194]").text(description); $(".js-view-count[data-work-id=111579194]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579194; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579194']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=111579194]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579194,"title":"Activity Assays of Thiadiazine Derivatives on \u0026lt;i\u0026gt;Trichomonas vaginalis \u0026lt;/i\u0026gt;and Amastigote Forms of \u0026lt;i\u0026gt;Trypanosoma cruzi\u0026lt;/i\u0026gt","translated_title":"","metadata":{"abstract":"Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. In vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. The capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. One of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronidazole, although higher doses were required. Its toxicity on cell proliferation in tissue culture was moderate. In vitro assays on amastigote forms of Trypanosoma cruzi were carried out using cultures of Vero cells infected with metacyclic trypomastigotes. For one compound (1) trypanocidal activity resembled that of nifurtimox as assessed by microscopic counts of infected and uninfected cells. Unfortunately, this compound showed a high degree of cytotoxicity on Vero cell cultures.","publisher":"Karger Publishers","publication_date":{"day":null,"month":null,"year":1992,"errors":{}},"publication_name":"Chemotherapy"},"translated_abstract":"Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. In vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. The capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. One of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronidazole, although higher doses were required. Its toxicity on cell proliferation in tissue culture was moderate. In vitro assays on amastigote forms of Trypanosoma cruzi were carried out using cultures of Vero cells infected with metacyclic trypomastigotes. For one compound (1) trypanocidal activity resembled that of nifurtimox as assessed by microscopic counts of infected and uninfected cells. Unfortunately, this compound showed a high degree of cytotoxicity on Vero cell cultures.","internal_url":"https://www.academia.edu/111579194/Activity_Assays_of_Thiadiazine_Derivatives_on_and_lt_i_and_gt_Trichomonas_vaginalis_and_lt_i_and_gt_and_Amastigote_Forms_of_and_lt_i_and_gt_Trypanosoma_cruzi_and_lt_i_and_gt","translated_internal_url":"","created_at":"2023-12-16T10:32:50.998-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Activity_Assays_of_Thiadiazine_Derivatives_on_and_lt_i_and_gt_Trichomonas_vaginalis_and_lt_i_and_gt_and_Amastigote_Forms_of_and_lt_i_and_gt_Trypanosoma_cruzi_and_lt_i_and_gt","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. In vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. The capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. One of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronidazole, although higher doses were required. Its toxicity on cell proliferation in tissue culture was moderate. In vitro assays on amastigote forms of Trypanosoma cruzi were carried out using cultures of Vero cells infected with metacyclic trypomastigotes. For one compound (1) trypanocidal activity resembled that of nifurtimox as assessed by microscopic counts of infected and uninfected cells. Unfortunately, this compound showed a high degree of cytotoxicity on Vero cell cultures.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":9114,"name":"Chemotherapy","url":"https://www.academia.edu/Documents/in/Chemotherapy"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":37782,"name":"Cell Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro"},{"id":66089,"name":"Tissue culture","url":"https://www.academia.edu/Documents/in/Tissue_culture"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":238630,"name":"Experimental Infection","url":"https://www.academia.edu/Documents/in/Experimental_Infection"},{"id":302037,"name":"In Vivo","url":"https://www.academia.edu/Documents/in/In_Vivo"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":619383,"name":"Benznidazole","url":"https://www.academia.edu/Documents/in/Benznidazole"},{"id":619386,"name":"Nifurtimox","url":"https://www.academia.edu/Documents/in/Nifurtimox"},{"id":744836,"name":"Trypanocidal Agents","url":"https://www.academia.edu/Documents/in/Trypanocidal_Agents"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":951639,"name":"Murine Model","url":"https://www.academia.edu/Documents/in/Murine_Model"},{"id":1303572,"name":"Metronidazole","url":"https://www.academia.edu/Documents/in/Metronidazole"},{"id":2469399,"name":"Thiadiazines","url":"https://www.academia.edu/Documents/in/Thiadiazines"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[{"id":37206603,"url":"https://doi.org/10.1159/000239040"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579190"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579190/Modulation_byPolypodium_leucotomosextract_of_cytokine_patterns_in_experimental_trichomoniasis_model"><img alt="Research paper thumbnail of Modulation byPolypodium leucotomosextract of cytokine patterns in experimental trichomoniasis model" class="work-thumbnail" src="https://attachments.academia-assets.com/109076950/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579190/Modulation_byPolypodium_leucotomosextract_of_cytokine_patterns_in_experimental_trichomoniasis_model">Modulation byPolypodium leucotomosextract of cytokine patterns in experimental trichomoniasis model</a></div><div class="wp-workCard_item"><span>Parasite</span><span>, Mar 1, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The immunomodulating effects of Anapsos®, an aqueous hydrosoluble extract obtained from the rhizo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The immunomodulating effects of Anapsos®, an aqueous hydrosoluble extract obtained from the rhizomes of the fern Polypodium leucotomos, on both pathogenicity and cytokine levels in serum (IFN-y/IL-4) were assayed in a Trichomonas vaginalis experimental model (BALB/c mice infected with 10 7 trichomonads and examined at day 15 after infection). Doses of 20 mg/kg/day administered for 10 days before the infection with the parasite induced a decrease of the experimental pathogenicity approximately 1 0-20 % compared to controls. Gross histopathologic changes at abdominal organs and mortality rale, as a consequence of pathogenicity of the protozoa and the immune response of the host, were evaluated. IFN-y and IL-4 cytokines were determined on days-5, 0, 5, 10, and 15 postinfection by indirect ELISA. Treatment with PAL before infection modulates and downregulates the IFN-y concentration, while anticipates and upregulates the IL-4 level. The assays performed have showed the utility of the murine model of experimental trichomoniasis for the evaluation of immunomodulatory activity of synthetic or natural products.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b779e431b32ebd6e47db17262a66be84" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076950,"asset_id":111579190,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076950/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579190"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579190"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579190; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579190]").text(description); $(".js-view-count[data-work-id=111579190]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579190; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579190']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b779e431b32ebd6e47db17262a66be84" } } $('.js-work-strip[data-work-id=111579190]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579190,"title":"Modulation byPolypodium leucotomosextract of cytokine patterns in experimental trichomoniasis model","translated_title":"","metadata":{"publisher":"EDP Sciences","ai_title_tag":"Polypodium leucotomos Extract in Trichomoniasis","grobid_abstract":"The immunomodulating effects of Anapsos®, an aqueous hydrosoluble extract obtained from the rhizomes of the fern Polypodium leucotomos, on both pathogenicity and cytokine levels in serum (IFN-y/IL-4) were assayed in a Trichomonas vaginalis experimental model (BALB/c mice infected with 10 7 trichomonads and examined at day 15 after infection). Doses of 20 mg/kg/day administered for 10 days before the infection with the parasite induced a decrease of the experimental pathogenicity approximately 1 0-20 % compared to controls. Gross histopathologic changes at abdominal organs and mortality rale, as a consequence of pathogenicity of the protozoa and the immune response of the host, were evaluated. IFN-y and IL-4 cytokines were determined on days-5, 0, 5, 10, and 15 postinfection by indirect ELISA. Treatment with PAL before infection modulates and downregulates the IFN-y concentration, while anticipates and upregulates the IL-4 level. The assays performed have showed the utility of the murine model of experimental trichomoniasis for the evaluation of immunomodulatory activity of synthetic or natural products.","publication_date":{"day":1,"month":3,"year":2003,"errors":{}},"publication_name":"Parasite","grobid_abstract_attachment_id":109076950},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579190/Modulation_byPolypodium_leucotomosextract_of_cytokine_patterns_in_experimental_trichomoniasis_model","translated_internal_url":"","created_at":"2023-12-16T10:32:50.513-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109076950,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076950/thumbnails/1.jpg","file_name":"parasite2003101p73.pdf","download_url":"https://www.academia.edu/attachments/109076950/download_file","bulk_download_file_name":"Modulation_byPolypodium_leucotomosextrac.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076950/parasite2003101p73-libre.pdf?1702752325=\u0026response-content-disposition=attachment%3B+filename%3DModulation_byPolypodium_leucotomosextrac.pdf\u0026Expires=1743089737\u0026Signature=CsDKmwcKzim8c-qB1EdTgdZhAQlT1LTnTkKqPLyDQP820zACoXnl3~DR1sw9lshJ33oUe6gHqdfwdwpZzhOMqi0pf3aJ1HPy7cCSexm0OZ8YabiLKIrdyLXri1ZUM74aF~6Odt1qniRZkfpQ6cmGv9fnHWOB6tcfF~hO5ew9et7hBjZzDJIz-luEIo5qxQnoZxBoVh562FL~Pu~G3bkldlRZu4em-Pi7KcNzXG~DC3bYOJjhrqtMz30CCmJNzW6b09Oo79nxJ-4lBSnMQy9FSMKX6R2PSLx1ulCvpKoW5S~WdtpuemBKObQfMITEjMryEu-Jt3Cn2NaTJ8Jdj8C8~A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Modulation_byPolypodium_leucotomosextract_of_cytokine_patterns_in_experimental_trichomoniasis_model","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"The immunomodulating effects of Anapsos®, an aqueous hydrosoluble extract obtained from the rhizomes of the fern Polypodium leucotomos, on both pathogenicity and cytokine levels in serum (IFN-y/IL-4) were assayed in a Trichomonas vaginalis experimental model (BALB/c mice infected with 10 7 trichomonads and examined at day 15 after infection). 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579187"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/111579187/The_relationship_of_experimental_pathogenicity_in_vivo_with_in_vitro_cytoadherence_and_cytotoxicity_of_6_different_isolates_of_Trichomonas_vaginalis"><img alt="Research paper thumbnail of The relationship of experimental pathogenicity in vivo with in vitro cytoadherence and cytotoxicity of 6 different isolates of Trichomonas vaginalis" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">The relationship of experimental pathogenicity in vivo with in vitro cytoadherence and cytotoxicity of 6 different isolates of Trichomonas vaginalis</div><div class="wp-workCard_item"><span>International Journal for Parasitology</span><span>, Aug 1, 1995</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas v...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas vaginalis were studied following their inoculation into NMRI mice and on to adherent cultures of HeLa cells. Contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. The strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate with experimental pathology in vivo.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579187"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579187"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579187; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579187]").text(description); $(".js-view-count[data-work-id=111579187]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579187; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579187']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=111579187]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579187,"title":"The relationship of experimental pathogenicity in vivo with in vitro cytoadherence and cytotoxicity of 6 different isolates of Trichomonas vaginalis","translated_title":"","metadata":{"abstract":"The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas vaginalis were studied following their inoculation into NMRI mice and on to adherent cultures of HeLa cells. Contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. The strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate with experimental pathology in vivo.","publisher":"Elsevier BV","publication_date":{"day":1,"month":8,"year":1995,"errors":{}},"publication_name":"International Journal for Parasitology"},"translated_abstract":"The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas vaginalis were studied following their inoculation into NMRI mice and on to adherent cultures of HeLa cells. Contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. The strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate with experimental pathology in vivo.","internal_url":"https://www.academia.edu/111579187/The_relationship_of_experimental_pathogenicity_in_vivo_with_in_vitro_cytoadherence_and_cytotoxicity_of_6_different_isolates_of_Trichomonas_vaginalis","translated_internal_url":"","created_at":"2023-12-16T10:32:50.016-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_relationship_of_experimental_pathogenicity_in_vivo_with_in_vitro_cytoadherence_and_cytotoxicity_of_6_different_isolates_of_Trichomonas_vaginalis","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas vaginalis were studied following their inoculation into NMRI mice and on to adherent cultures of HeLa cells. Contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. The strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate with experimental pathology in vivo.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":164,"name":"Parasitology","url":"https://www.academia.edu/Documents/in/Parasitology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":10055,"name":"Cell Adhesion","url":"https://www.academia.edu/Documents/in/Cell_Adhesion"},{"id":22050,"name":"Cytotoxicity","url":"https://www.academia.edu/Documents/in/Cytotoxicity"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":302037,"name":"In Vivo","url":"https://www.academia.edu/Documents/in/In_Vivo"},{"id":422325,"name":"HeLa cells","url":"https://www.academia.edu/Documents/in/HeLa_cells"},{"id":608958,"name":"HeLa","url":"https://www.academia.edu/Documents/in/HeLa"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":4063964,"name":"Trichomonas","url":"https://www.academia.edu/Documents/in/Trichomonas"}],"urls":[{"id":37206596,"url":"https://doi.org/10.1016/0020-7519(95)00001-i"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579183"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579183/Experimental_models_in_Chagas_disease_a_review_of_the_methodologies_applied_for_screening_compounds_against_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Experimental models in Chagas disease: a review of the methodologies applied for screening compounds against Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/109077003/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579183/Experimental_models_in_Chagas_disease_a_review_of_the_methodologies_applied_for_screening_compounds_against_Trypanosoma_cruzi">Experimental models in Chagas disease: a review of the methodologies applied for screening compounds against Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>Parasitology Research</span><span>, Sep 19, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">One of the main problems of Chagas disease (CD), the parasitic infection caused by Trypanosoma cr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">One of the main problems of Chagas disease (CD), the parasitic infection caused by Trypanosoma cruzi, is the lack of a completely satisfactory treatment, which is currently based on two old nitroheterocyclic drugs (i.e., nifurtimox and benznidazole) that show important limitations for treating patients. In this context, many laboratories look for alternative therapies potentially applicable to the treatment, and therefore, research in CD chemotherapy works in the design of experimental protocols for detecting molecules with activity against T. cruzi. Phenotypic assays are considered the most valuable strategy for screening these antiparasitic compounds. Among them, in vitro experiments are the first step to test potential anti-T. cruzi drugs directly on the different parasite forms (i.e., epimastigotes, trypomastigotes, and amastigotes) and to detect cytotoxicity. Once the putative trypanocidal drug has been identified in vitro, it must be moved to in vivo models of T. cruzi infection, to explore (i) acute toxicity, (ii) efficacy during the acute infection, and (iii) efficacy in the chronic disease. Moreover, in silico approaches for predicting activity have emerged as a supporting tool for drug screening procedures. Accordingly, this work reviews those in vitro, in vivo, and in silico methods that have been routinely applied during the last decades, aiming to discover trypanocidal compounds that contribute to developing more effective CD treatments.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4fa498ad4f3aaac6313832bdf583a4ac" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077003,"asset_id":111579183,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077003/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579183"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579183"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579183; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579183]").text(description); $(".js-view-count[data-work-id=111579183]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579183; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579183']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4fa498ad4f3aaac6313832bdf583a4ac" } } $('.js-work-strip[data-work-id=111579183]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579183,"title":"Experimental models in Chagas disease: a review of the methodologies applied for screening compounds against Trypanosoma cruzi","translated_title":"","metadata":{"publisher":"Springer Science+Business Media","grobid_abstract":"One of the main problems of Chagas disease (CD), the parasitic infection caused by Trypanosoma cruzi, is the lack of a completely satisfactory treatment, which is currently based on two old nitroheterocyclic drugs (i.e., nifurtimox and benznidazole) that show important limitations for treating patients. In this context, many laboratories look for alternative therapies potentially applicable to the treatment, and therefore, research in CD chemotherapy works in the design of experimental protocols for detecting molecules with activity against T. cruzi. Phenotypic assays are considered the most valuable strategy for screening these antiparasitic compounds. Among them, in vitro experiments are the first step to test potential anti-T. cruzi drugs directly on the different parasite forms (i.e., epimastigotes, trypomastigotes, and amastigotes) and to detect cytotoxicity. Once the putative trypanocidal drug has been identified in vitro, it must be moved to in vivo models of T. cruzi infection, to explore (i) acute toxicity, (ii) efficacy during the acute infection, and (iii) efficacy in the chronic disease. Moreover, in silico approaches for predicting activity have emerged as a supporting tool for drug screening procedures. 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These compounds were subsequently evaluated as inhibitors of biocrystallization of ferriprotoporphyrin IX (heme) to hemozoin, a Plasmodium detoxification specific process. Most bis(5-nitroindazol-3-ols) were good inhibitors, however, a denitro analogue (38), the intermediate bis(3-alkoxyindazoles) (15-29) as well as bis(indazolin-3-ones) (39-42) were not active, showing the importance of the NO(2) and OH groups in the inhibition process.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579181"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579181"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579181; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579181]").text(description); $(".js-view-count[data-work-id=111579181]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579181; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579181']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=111579181]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579181,"title":"Synthesis and Evaluation of 1,1′‐Hydrocarbylenebis(indazol‐3‐ols) as Potential Antimalarial Drugs","translated_title":"","metadata":{"abstract":"Bis(indazol-3-ol) derivatives (5, 30-38) were prepared by alkylation of 3-alkoxyindazoles with alpha,omega-dibromides, followed by removal of the O-protecting groups. 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Most bis(5-nitroindazol-3-ols) were good inhibitors, however, a denitro analogue (38), the intermediate bis(3-alkoxyindazoles) (15-29) as well as bis(indazolin-3-ones) (39-42) were not active, showing the importance of the NO(2) and OH groups in the inhibition process.","internal_url":"https://www.academia.edu/111579181/Synthesis_and_Evaluation_of_1_1_Hydrocarbylenebis_indazol_3_ols_as_Potential_Antimalarial_Drugs","translated_internal_url":"","created_at":"2023-12-16T10:32:47.390-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Synthesis_and_Evaluation_of_1_1_Hydrocarbylenebis_indazol_3_ols_as_Potential_Antimalarial_Drugs","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Bis(indazol-3-ol) derivatives (5, 30-38) were prepared by alkylation of 3-alkoxyindazoles with alpha,omega-dibromides, followed by removal of the O-protecting groups. These compounds were subsequently evaluated as inhibitors of biocrystallization of ferriprotoporphyrin IX (heme) to hemozoin, a Plasmodium detoxification specific process. Most bis(5-nitroindazol-3-ols) were good inhibitors, however, a denitro analogue (38), the intermediate bis(3-alkoxyindazoles) (15-29) as well as bis(indazolin-3-ones) (39-42) were not active, showing the importance of the NO(2) and OH groups in the inhibition process.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":531,"name":"Organic Chemistry","url":"https://www.academia.edu/Documents/in/Organic_Chemistry"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":30696,"name":"Hemozoin","url":"https://www.academia.edu/Documents/in/Hemozoin"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":204375,"name":"Heme","url":"https://www.academia.edu/Documents/in/Heme"},{"id":454707,"name":"Antimalarials","url":"https://www.academia.edu/Documents/in/Antimalarials"},{"id":664186,"name":"Alkylation","url":"https://www.academia.edu/Documents/in/Alkylation"},{"id":1330008,"name":"Hemin","url":"https://www.academia.edu/Documents/in/Hemin"},{"id":1692059,"name":"Minimum inhibitory concentration","url":"https://www.academia.edu/Documents/in/Minimum_inhibitory_concentration"},{"id":1693871,"name":"Plasmodium berghei","url":"https://www.academia.edu/Documents/in/Plasmodium_berghei"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"},{"id":4148248,"name":"Minimum Inhibitory Concentration","url":"https://www.academia.edu/Documents/in/Minimum_Inhibitory_Concentration-1"}],"urls":[{"id":37206592,"url":"https://doi.org/10.1002/cmdc.200800176"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579180"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579180/SHORT_COMMUNICATION_Biological_Variability_in_Clinical_Isolates_of_Trichomonas_vaginalis"><img alt="Research paper thumbnail of SHORT COMMUNICATION - Biological Variability in Clinical Isolates of Trichomonas vaginalis" class="work-thumbnail" src="https://attachments.academia-assets.com/109076996/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579180/SHORT_COMMUNICATION_Biological_Variability_in_Clinical_Isolates_of_Trichomonas_vaginalis">SHORT COMMUNICATION - Biological Variability in Clinical Isolates of Trichomonas vaginalis</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Goverment of Madrid. A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fca7b57eb49119c2bb6ff70c5294d9cc" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076996,"asset_id":111579180,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076996/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579180"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579180"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579180; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579180]").text(description); $(".js-view-count[data-work-id=111579180]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579180; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579180']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fca7b57eb49119c2bb6ff70c5294d9cc" } } $('.js-work-strip[data-work-id=111579180]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579180,"title":"SHORT COMMUNICATION - Biological Variability in Clinical Isolates of Trichomonas vaginalis","translated_title":"","metadata":{"grobid_abstract":"Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Goverment of Madrid. 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A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109076996,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076996/thumbnails/1.jpg","file_name":"4525.pdf","download_url":"https://www.academia.edu/attachments/109076996/download_file","bulk_download_file_name":"SHORT_COMMUNICATION_Biological_Variabili.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076996/4525-libre.pdf?1702752316=\u0026response-content-disposition=attachment%3B+filename%3DSHORT_COMMUNICATION_Biological_Variabili.pdf\u0026Expires=1743089737\u0026Signature=ckkqohoP9envUAll8WfBoYV5Wbj0EDISJWJSwmETqmPODr9f6~Vvdu6ucni2BKGCII5UIaXjkVydeFeBJ8-OW~PKKuuKJnzt8ny4ZrQRG6ICAyM6UlpKcsOEOU35H-kjO~tMy7m3-o-AGtau2BstgONMqriXu1bVMYyYu5a1FVINKxBPxieyiaBPXZPbbm4b7oQxi~AVfHokRt3BwDlO4etiMuLzp2WJrj-~klOh8izqFAEzMMA0WmfztZ9SWsZ-b1z1IhH0-v66WeOCv87MzSEfXY5ijtZcMs3qo8FovSxEU20vztgtoKBb25L9qHG2pYrEBjYs~kXPqYaughhf4w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":1760295,"name":"Trichomoniasis","url":"https://www.academia.edu/Documents/in/Trichomoniasis"},{"id":4063964,"name":"Trichomonas","url":"https://www.academia.edu/Documents/in/Trichomonas"}],"urls":[{"id":37206590,"url":"https://tspace.library.utoronto.ca/bitstream/1807/20170/4/oc02171.html"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579178"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579178/ChemInform_Abstract_New_Derivatives_of_5_Nitroimidazole_Synthesis_and_Antiparasitic_Activity"><img alt="Research paper thumbnail of ChemInform Abstract: New Derivatives of 5-Nitroimidazole: Synthesis and Antiparasitic Activity" class="work-thumbnail" src="https://attachments.academia-assets.com/109076994/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579178/ChemInform_Abstract_New_Derivatives_of_5_Nitroimidazole_Synthesis_and_Antiparasitic_Activity">ChemInform Abstract: New Derivatives of 5-Nitroimidazole: Synthesis and Antiparasitic Activity</a></div><div class="wp-workCard_item"><span>ChemInform</span><span>, Jun 26, 1990</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ChemInform Abstract In the search for a model tetrahydropteridine which is stable in air and solu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ChemInform Abstract In the search for a model tetrahydropteridine which is stable in air and soluble in organic solvents, the title pteridine derivative (IX) which shows the expected properties is synthesized. (IX) is prepared via the intermediate (V) which can be obtained via two different routes starting from ethyl 5-nitroorotate (I). The second route involves the unexpected formation of the ester (VII).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d64384ab0bacd8f2ddea9155d2080917" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076994,"asset_id":111579178,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076994/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579178"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579178"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579178; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579178]").text(description); $(".js-view-count[data-work-id=111579178]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579178; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579178']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d64384ab0bacd8f2ddea9155d2080917" } } $('.js-work-strip[data-work-id=111579178]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579178,"title":"ChemInform Abstract: New Derivatives of 5-Nitroimidazole: Synthesis and Antiparasitic Activity","translated_title":"","metadata":{"publisher":"Wiley","grobid_abstract":"ChemInform Abstract In the search for a model tetrahydropteridine which is stable in air and soluble in organic solvents, the title pteridine derivative (IX) which shows the expected properties is synthesized. 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(IX) is prepared via the intermediate (V) which can be obtained via two different routes starting from ethyl 5-nitroorotate (I). 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579175"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/111579175/On_the_experimental_activity_of_new_gentian_violet_crystal_violet_derivatives_on_trypanosoma_cruzi"><img alt="Research paper thumbnail of On the experimental activity of new gentian violet crystal violet derivatives on trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">On the experimental activity of new gentian violet crystal violet derivatives on trypanosoma cruzi</div><div class="wp-workCard_item"><span>Revista de Ciencias Farmaceuticas</span><span>, Jun 23, 1991</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579175"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579175"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579175; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579174"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579174/Activity_profile_of_two_5_nitroindazole_derivatives_over_the_moderately_drug_resistant_Trypanosoma_cruzi_Y_strain_DTU_TcII_in_vitro_and_in_vivo_studies"><img alt="Research paper thumbnail of Activity profile of two 5-nitroindazole derivatives over the moderately drug-resistant Trypanosoma cruzi Y strain (DTU TcII): in vitro and in vivo studies" class="work-thumbnail" src="https://attachments.academia-assets.com/109076992/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579174/Activity_profile_of_two_5_nitroindazole_derivatives_over_the_moderately_drug_resistant_Trypanosoma_cruzi_Y_strain_DTU_TcII_in_vitro_and_in_vivo_studies">Activity profile of two 5-nitroindazole derivatives over the moderately drug-resistant Trypanosoma cruzi Y strain (DTU TcII): in vitro and in vivo studies</a></div><div class="wp-workCard_item"><span>Parasitology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In previous studies, we have identified several families of 5-nitroindazole derivatives as promis...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In previous studies, we have identified several families of 5-nitroindazole derivatives as promising antichagasic prototypes. Among them, 1-(2-aminoethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one, (hydrochloride) and 1-(2-acetoxyethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one (compounds 16 and 24, respectively) have recently shown outstanding activity in vitro over the drug-sensitive Trypanosoma cruzi CL strain (DTU TcVI). Here, we explored the activity of these derivatives against the moderately drug-resistant Y strain (DTU TcII), in vitro and in vivo. The outcomes confirmed their activity over replicative forms, showing IC50 values of 0.49 (16) and 5.75 μm (24) towards epimastigotes, 0.41 (16) and 1.17 μm (24) against intracellular amastigotes. These results, supported by the lack of toxicity on cardiac cells, led to better selectivities than benznidazole (BZ). Otherwise, they were not as active as BZ in vitro against the non-replicative form of the parasite, i.e. bloods...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7343c51f7ce8674625af723164fb1a09" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076992,"asset_id":111579174,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076992/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579174"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579174"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579174; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579174]").text(description); $(".js-view-count[data-work-id=111579174]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579174; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579174']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7343c51f7ce8674625af723164fb1a09" } } $('.js-work-strip[data-work-id=111579174]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579174,"title":"Activity profile of two 5-nitroindazole derivatives over the moderately drug-resistant Trypanosoma cruzi Y strain (DTU TcII): in vitro and in vivo studies","translated_title":"","metadata":{"abstract":"In previous studies, we have identified several families of 5-nitroindazole derivatives as promising antichagasic prototypes. Among them, 1-(2-aminoethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one, (hydrochloride) and 1-(2-acetoxyethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one (compounds 16 and 24, respectively) have recently shown outstanding activity in vitro over the drug-sensitive Trypanosoma cruzi CL strain (DTU TcVI). Here, we explored the activity of these derivatives against the moderately drug-resistant Y strain (DTU TcII), in vitro and in vivo. The outcomes confirmed their activity over replicative forms, showing IC50 values of 0.49 (16) and 5.75 μm (24) towards epimastigotes, 0.41 (16) and 1.17 μm (24) against intracellular amastigotes. These results, supported by the lack of toxicity on cardiac cells, led to better selectivities than benznidazole (BZ). Otherwise, they were not as active as BZ in vitro against the non-replicative form of the parasite, i.e. bloods...","publisher":"Cambridge University Press (CUP)","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Parasitology"},"translated_abstract":"In previous studies, we have identified several families of 5-nitroindazole derivatives as promising antichagasic prototypes. Among them, 1-(2-aminoethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one, (hydrochloride) and 1-(2-acetoxyethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one (compounds 16 and 24, respectively) have recently shown outstanding activity in vitro over the drug-sensitive Trypanosoma cruzi CL strain (DTU TcVI). Here, we explored the activity of these derivatives against the moderately drug-resistant Y strain (DTU TcII), in vitro and in vivo. The outcomes confirmed their activity over replicative forms, showing IC50 values of 0.49 (16) and 5.75 μm (24) towards epimastigotes, 0.41 (16) and 1.17 μm (24) against intracellular amastigotes. These results, supported by the lack of toxicity on cardiac cells, led to better selectivities than benznidazole (BZ). 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Among them, 1-(2-aminoethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one, (hydrochloride) and 1-(2-acetoxyethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one (compounds 16 and 24, respectively) have recently shown outstanding activity in vitro over the drug-sensitive Trypanosoma cruzi CL strain (DTU TcVI). Here, we explored the activity of these derivatives against the moderately drug-resistant Y strain (DTU TcII), in vitro and in vivo. The outcomes confirmed their activity over replicative forms, showing IC50 values of 0.49 (16) and 5.75 μm (24) towards epimastigotes, 0.41 (16) and 1.17 μm (24) against intracellular amastigotes. These results, supported by the lack of toxicity on cardiac cells, led to better selectivities than benznidazole (BZ). Otherwise, they were not as active as BZ in vitro against the non-replicative form of the parasite, i.e. bloods...","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109076992,"title":"","file_type":"txt","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076992/thumbnails/1.jpg","file_name":"501100004586.txt","download_url":"https://www.academia.edu/attachments/109076992/download_file","bulk_download_file_name":"Activity_profile_of_two_5_nitroindazole.txt","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076992/501100004586.txt?1702751625=\u0026response-content-disposition=attachment%3B+filename%3DActivity_profile_of_two_5_nitroindazole.txt\u0026Expires=1743089737\u0026Signature=BCf0hktGdhWufdcZVlN82d348V1E0p7npMsfoDhlxy54EpnOBdklWVlVQr-AUBjeKb2fhkB2j9IWYffe1m8pJrz8IgT3sUUkJXS1wn5oM4YrTCPBsXYUNQoro0xmM1lPHfGzyV0Kq1xG7ThJ8FkVqxNpXDeKSA9DGqDAt6r9MLQKnP2S5AZoDjo9-05wSwWI9PVGArrOXQ7mXd8eQYxxJ7tD1STjjPVYvlY2yZid4cTpNKhEM5czGDJ54csKNGPLqlu6-sTuAkqbTIDLTNPyegPAZqkCd54IWq6962ZCSN8H63f-21-U9~8WZA5u7r93WdeRhJy~bl5fzLsMF7ZLVw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology"},{"id":164,"name":"Parasitology","url":"https://www.academia.edu/Documents/in/Parasitology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":9114,"name":"Chemotherapy","url":"https://www.academia.edu/Documents/in/Chemotherapy"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro"},{"id":67405,"name":"Toxicity","url":"https://www.academia.edu/Documents/in/Toxicity"},{"id":225340,"name":"Chagas disease","url":"https://www.academia.edu/Documents/in/Chagas_disease"},{"id":302037,"name":"In Vivo","url":"https://www.academia.edu/Documents/in/In_Vivo"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":619383,"name":"Benznidazole","url":"https://www.academia.edu/Documents/in/Benznidazole"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"}],"urls":[{"id":37206586,"url":"https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182020000955"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="5001198" id="papers"><div class="js-work-strip profile--work_container" data-work-id="112332326"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/112332326/Simulaci%C3%B3n_virtual_e_lab_de_las_pr%C3%A1cticas_de_Fundamentos_y_T%C3%A9cnicas_Inmunol%C3%B3gicas"><img alt="Research paper thumbnail of Simulación virtual (e-lab) de las prácticas de Fundamentos y Técnicas Inmunológicas" class="work-thumbnail" src="https://attachments.academia-assets.com/109595130/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/112332326/Simulaci%C3%B3n_virtual_e_lab_de_las_pr%C3%A1cticas_de_Fundamentos_y_T%C3%A9cnicas_Inmunol%C3%B3gicas">Simulación virtual (e-lab) de las prácticas de Fundamentos y Técnicas Inmunológicas</a></div><div class="wp-workCard_item"><span>RELADA - Revista Electrónica de ADA-Madrid</span><span>, Dec 3, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Resumen: En el campo de la enseñanza de materias experimentales, el mayor coste de las clases prá...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Resumen: En el campo de la enseñanza de materias experimentales, el mayor coste de las clases prácticas, la necesidad de impartirlas en grupos más reducidos y la falta material de espacio y tiempo, ha provocado la pérdida de la coordinación teoría/practica y, consecuentemente, el concepto integral de la asignatura. Aprovechando el enorme desarrollo que han experimentado las aplicaciones multimedia y "applets", que conjugan la animación con interactividad y la aplicación de las nuevas técnicas, hemos querido efectuar un diseño interactivo en 3D, mediante el cual el discente pueda realizar las practicas reales de forma virtual. Se ha realizado, para la asignatura de Inmunología, la simulación de una práctica (Inmunofluorescencia) de forma completa en un laboratorio virtual. El discente, conociendo qué metodología debe aplicar, debe escoger tanto los reactivos como los materiales necesarios para llevar a cabo la práctica. Finalmente el alumno recibirá una puntuación en base a los errores cometidos durante su ejecución.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="434409b8e28cb0e93fd50c9c9f9e4d8f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109595130,"asset_id":112332326,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109595130/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="112332326"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="112332326"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 112332326; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=112332326]").text(description); $(".js-view-count[data-work-id=112332326]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 112332326; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='112332326']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "434409b8e28cb0e93fd50c9c9f9e4d8f" } } $('.js-work-strip[data-work-id=112332326]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":112332326,"title":"Simulación virtual (e-lab) de las prácticas de Fundamentos y Técnicas Inmunológicas","translated_title":"","metadata":{"grobid_abstract":"Resumen: En el campo de la enseñanza de materias experimentales, el mayor coste de las clases prácticas, la necesidad de impartirlas en grupos más reducidos y la falta material de espacio y tiempo, ha provocado la pérdida de la coordinación teoría/practica y, consecuentemente, el concepto integral de la asignatura. Aprovechando el enorme desarrollo que han experimentado las aplicaciones multimedia y \"applets\", que conjugan la animación con interactividad y la aplicación de las nuevas técnicas, hemos querido efectuar un diseño interactivo en 3D, mediante el cual el discente pueda realizar las practicas reales de forma virtual. Se ha realizado, para la asignatura de Inmunología, la simulación de una práctica (Inmunofluorescencia) de forma completa en un laboratorio virtual. El discente, conociendo qué metodología debe aplicar, debe escoger tanto los reactivos como los materiales necesarios para llevar a cabo la práctica. 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There was an increase in the stratified epithelium layers as well as edema produced by the increase of vascularization in the propia submucosa and infiltration of leukocytes. The proliferation of the vaginal epithelium favors the settlement and persistence of the parasitic infection. All of the findings corresponded with signs of a systemic disease being observed in the animals, including significant weight loss and also intestinal invasion. The entire inflammatory process has been corroborated by studies of adhesion molecules such as E-Selectin, VCAM-1 and PECAM-1. A correlation between the time of appearance and the perseverance of the inflammatory process with E-Selectin and VCAM-1 expression was observed, but not with PECAM-1. The strain with a higher pathogenicity was able to invade deep vaginal tissues and thus, parasites could not be detected by vaginal washings. This may be an important cause of diagnosis and treatment failure. Also, by the different localization of trichomonads, it appeared that the battle between host and parasite took place in different areas dependent upon the characteristics of the strain.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6c2d7cb883401661eaf017dfa6c355d2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077724,"asset_id":111580331,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077724/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111580331"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111580331"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111580331; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111580331]").text(description); $(".js-view-count[data-work-id=111580331]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111580331; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111580331']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6c2d7cb883401661eaf017dfa6c355d2" } } $('.js-work-strip[data-work-id=111580331]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111580331,"title":"Immunohistochemical study of the vaginal inflammatory response in experimental trichomoniasis","translated_title":"","metadata":{"publisher":"Elsevier BV","ai_title_tag":"Vaginal Inflammatory Response in Trichomoniasis","grobid_abstract":"In the present paper, the acute and subchronical inflammatory processes of the vaginal epithelial were studied in mice experimentally infected with two Trichomonas vaginalis strains of different pathogenicity, by means of histological and immunological methods. 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Cluster analysis by UPGMA (unweighted pair group method analysis) was then applied both to biological parameters and RAPD profiles. Inspection of the UPGMA phenograms indicates identical clusters, so supporting that usefulness of biological parameters to characterization of T. cruzi strains still remains.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="73ba75b0797795d039bca5c542f6e985" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076960,"asset_id":111579215,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076960/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579215"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579215"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579215; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579215]").text(description); $(".js-view-count[data-work-id=111579215]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579215; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579215']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "73ba75b0797795d039bca5c542f6e985" } } $('.js-work-strip[data-work-id=111579215]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579215,"title":"Relationship between biological behaviour and randomly amplified polymorphic DNA profiles of Trypanosoma cruzi strains","translated_title":"","metadata":{"publisher":"Instituto Oswaldo Cruz, Ministério da Saúde","grobid_abstract":"Once known some biological characteristics of six Trypanosoma cruzi strains, randomly amplified polymorphic DNA (RAPD) analysis was made. 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Inspection of the UPGMA phenograms indicates identical clusters, so supporting that usefulness of biological parameters to characterization of T. cruzi strains still remains.","publication_date":{"day":1,"month":2,"year":2001,"errors":{}},"publication_name":"Memorias Do Instituto Oswaldo Cruz","grobid_abstract_attachment_id":109076960},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579215/Relationship_between_biological_behaviour_and_randomly_amplified_polymorphic_DNA_profiles_of_Trypanosoma_cruzi_strains","translated_internal_url":"","created_at":"2023-12-16T10:32:55.810-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109076960,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076960/thumbnails/1.jpg","file_name":"DM6HcJpKDQXr7n88436QQ3S.pdf","download_url":"https://www.academia.edu/attachments/109076960/download_file","bulk_download_file_name":"Relationship_between_biological_behaviou.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076960/DM6HcJpKDQXr7n88436QQ3S-libre.pdf?1702752322=\u0026response-content-disposition=attachment%3B+filename%3DRelationship_between_biological_behaviou.pdf\u0026Expires=1743089736\u0026Signature=gmIXipQaSuYVkpM-0GpUi~359z0pFu-4y3XVS8T6U67s0kx5nunnhq0PQBlD3KpoM18d5QdOaTdaTw1Pk9Eu4turbWWwedi~pvn6enmzQAePWbIyUW6bx6P1VDfaEdJ8FVOAv2hBXQH0sExAkug9iqAhFEcE2tUjJN8xT7foDWXLrT3f6m-J1Y5rJ98xBC3pMcfV6eTX7mVOvibHTCPQi0ksm3hBiITRotAeeTK0EMF1PteJbD1l2kYIh37aY6pOt2r1M8jsVYM810DoRwd23JMcaCk-Od5xJrDez8KQ2jrp7iySiftJreaXhChOEEDbq~MOj4N0dzsQGoGHlWe3DA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Relationship_between_biological_behaviour_and_randomly_amplified_polymorphic_DNA_profiles_of_Trypanosoma_cruzi_strains","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"Once known some biological characteristics of six Trypanosoma cruzi strains, randomly amplified polymorphic DNA (RAPD) analysis was made. 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Inspection of the UPGMA phenograms indicates identical clusters, so supporting that usefulness of biological parameters to characterization of T. cruzi strains still remains.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109076960,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076960/thumbnails/1.jpg","file_name":"DM6HcJpKDQXr7n88436QQ3S.pdf","download_url":"https://www.academia.edu/attachments/109076960/download_file","bulk_download_file_name":"Relationship_between_biological_behaviou.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076960/DM6HcJpKDQXr7n88436QQ3S-libre.pdf?1702752322=\u0026response-content-disposition=attachment%3B+filename%3DRelationship_between_biological_behaviou.pdf\u0026Expires=1743089736\u0026Signature=gmIXipQaSuYVkpM-0GpUi~359z0pFu-4y3XVS8T6U67s0kx5nunnhq0PQBlD3KpoM18d5QdOaTdaTw1Pk9Eu4turbWWwedi~pvn6enmzQAePWbIyUW6bx6P1VDfaEdJ8FVOAv2hBXQH0sExAkug9iqAhFEcE2tUjJN8xT7foDWXLrT3f6m-J1Y5rJ98xBC3pMcfV6eTX7mVOvibHTCPQi0ksm3hBiITRotAeeTK0EMF1PteJbD1l2kYIh37aY6pOt2r1M8jsVYM810DoRwd23JMcaCk-Od5xJrDez8KQ2jrp7iySiftJreaXhChOEEDbq~MOj4N0dzsQGoGHlWe3DA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":2749,"name":"Animal Behavior","url":"https://www.academia.edu/Documents/in/Animal_Behavior"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA"},{"id":153640,"name":"RAPD","url":"https://www.academia.edu/Documents/in/RAPD"},{"id":193456,"name":"UPGMA","url":"https://www.academia.edu/Documents/in/UPGMA"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":2547626,"name":"genetic heterogeneity","url":"https://www.academia.edu/Documents/in/genetic_heterogeneity"}],"urls":[{"id":37206621,"url":"https://www.scielo.br/j/mioc/a/DM6HcJpKDQXr7n88436QQ3S/?lang=en\u0026format=pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579212"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579212/Effect_of_piroxicam_metamizol_and_S_adenosylmethionine_in_a_murine_model_of_experimental_trichomoniasis"><img alt="Research paper thumbnail of Effect of piroxicam, metamizol, and S-adenosylmethionine in a murine model of experimental trichomoniasis" class="work-thumbnail" src="https://attachments.academia-assets.com/109077013/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579212/Effect_of_piroxicam_metamizol_and_S_adenosylmethionine_in_a_murine_model_of_experimental_trichomoniasis">Effect of piroxicam, metamizol, and S-adenosylmethionine in a murine model of experimental trichomoniasis</a></div><div class="wp-workCard_item"><span>Parasite</span><span>, Mar 1, 2005</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Biological effects of piroxicam, metamizol, and S-adenosylmethionine (S-AMET) have been tested in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Biological effects of piroxicam, metamizol, and S-adenosylmethionine (S-AMET) have been tested in NMRI mice infected intraperitoneally with Trichomonas vaginalis. An intraperitoneal treatment during ten preinfection days with piroxicam (10 mg/Kg/day), or metamizol (275 mg/Kg/day), but not with S-AMET (17 mg/Kg/day) induced a significant decrease of abdominal lesions and mortality, assessed by means of a pathogenicity index. The trichomonicidal activity of piroxicam, metamizol, and S-AMET was tested in vitro at the concentration of 300 µM, but found ineffective. These assays have shown the usefulness of the experimental trichomoniasis model for the study of the immunomodulating activity of synthetic drugs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e30b0d92aac1ccbcded10bb44b1edf8b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077013,"asset_id":111579212,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077013/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579212"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579212"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579212; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579212]").text(description); $(".js-view-count[data-work-id=111579212]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579212; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579212']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e30b0d92aac1ccbcded10bb44b1edf8b" } } $('.js-work-strip[data-work-id=111579212]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579212,"title":"Effect of piroxicam, metamizol, and S-adenosylmethionine in a murine model of experimental trichomoniasis","translated_title":"","metadata":{"publisher":"EDP Sciences","grobid_abstract":"Biological effects of piroxicam, metamizol, and S-adenosylmethionine (S-AMET) have been tested in NMRI mice infected intraperitoneally with Trichomonas vaginalis. An intraperitoneal treatment during ten preinfection days with piroxicam (10 mg/Kg/day), or metamizol (275 mg/Kg/day), but not with S-AMET (17 mg/Kg/day) induced a significant decrease of abdominal lesions and mortality, assessed by means of a pathogenicity index. The trichomonicidal activity of piroxicam, metamizol, and S-AMET was tested in vitro at the concentration of 300 µM, but found ineffective. These assays have shown the usefulness of the experimental trichomoniasis model for the study of the immunomodulating activity of synthetic drugs.","publication_date":{"day":1,"month":3,"year":2005,"errors":{}},"publication_name":"Parasite","grobid_abstract_attachment_id":109077013},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579212/Effect_of_piroxicam_metamizol_and_S_adenosylmethionine_in_a_murine_model_of_experimental_trichomoniasis","translated_internal_url":"","created_at":"2023-12-16T10:32:55.125-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109077013,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077013/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/109077013/download_file","bulk_download_file_name":"Effect_of_piroxicam_metamizol_and_S_aden.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077013/pdf-libre.pdf?1702752317=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_piroxicam_metamizol_and_S_aden.pdf\u0026Expires=1743089736\u0026Signature=YgUjNzfVNHF7fJaHes~Q4kVyq-fZhlPtcatF10aQIBYzQ0lNP1L12fbh1sjYMZJWCf0Rtvuaeq5oqToLjsxlLfeJewv9UZtQHuiH5xvxYrseJ5Xr8j6eyYPRYjUTdlBc8tfQJqB2CjStV3ymTLruYT41Z5CvsWwbSpKdsxrWvwUgSM6rclW2GDVguZuLYsNuLzTVIq7jy91XvJ6fqepVqxloKRDLrLzdZgVpAJE1efRLuwhP9rZCv91WRk7W9-croaVrS~xx10NGaKFT357vxX7waJ-CV1fLRISWe4GgLHZB18neBxTEyIo3uHbYJevyr-LzfVNfuBaoXqViRogjXw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Effect_of_piroxicam_metamizol_and_S_adenosylmethionine_in_a_murine_model_of_experimental_trichomoniasis","translated_slug":"","page_count":5,"language":"en","content_type":"Work","summary":"Biological effects of piroxicam, metamizol, and S-adenosylmethionine (S-AMET) have been tested in NMRI mice infected intraperitoneally with Trichomonas vaginalis. An intraperitoneal treatment during ten preinfection days with piroxicam (10 mg/Kg/day), or metamizol (275 mg/Kg/day), but not with S-AMET (17 mg/Kg/day) induced a significant decrease of abdominal lesions and mortality, assessed by means of a pathogenicity index. The trichomonicidal activity of piroxicam, metamizol, and S-AMET was tested in vitro at the concentration of 300 µM, but found ineffective. These assays have shown the usefulness of the experimental trichomoniasis model for the study of the immunomodulating activity of synthetic drugs.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109077013,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077013/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/109077013/download_file","bulk_download_file_name":"Effect_of_piroxicam_metamizol_and_S_aden.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077013/pdf-libre.pdf?1702752317=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_piroxicam_metamizol_and_S_aden.pdf\u0026Expires=1743089736\u0026Signature=YgUjNzfVNHF7fJaHes~Q4kVyq-fZhlPtcatF10aQIBYzQ0lNP1L12fbh1sjYMZJWCf0Rtvuaeq5oqToLjsxlLfeJewv9UZtQHuiH5xvxYrseJ5Xr8j6eyYPRYjUTdlBc8tfQJqB2CjStV3ymTLruYT41Z5CvsWwbSpKdsxrWvwUgSM6rclW2GDVguZuLYsNuLzTVIq7jy91XvJ6fqepVqxloKRDLrLzdZgVpAJE1efRLuwhP9rZCv91WRk7W9-croaVrS~xx10NGaKFT357vxX7waJ-CV1fLRISWe4GgLHZB18neBxTEyIo3uHbYJevyr-LzfVNfuBaoXqViRogjXw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":94271,"name":"Parasite","url":"https://www.academia.edu/Documents/in/Parasite"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":734759,"name":"PIROXICAM","url":"https://www.academia.edu/Documents/in/PIROXICAM"},{"id":923704,"name":"S-Adenosylmethionine","url":"https://www.academia.edu/Documents/in/S-Adenosylmethionine"},{"id":1281483,"name":"Ascites","url":"https://www.academia.edu/Documents/in/Ascites"},{"id":1760295,"name":"Trichomoniasis","url":"https://www.academia.edu/Documents/in/Trichomoniasis"},{"id":2463495,"name":"Random Allocation","url":"https://www.academia.edu/Documents/in/Random_Allocation"},{"id":4110060,"name":"Dipyrone","url":"https://www.academia.edu/Documents/in/Dipyrone"}],"urls":[{"id":37206618,"url":"https://doi.org/10.1051/parasite/2005121079"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579209"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/111579209/Evaluation_of_drug_activity_against_intracellular_forms_of_Trypanosoma_cruzi_employing_enzyme_immunoassay"><img alt="Research paper thumbnail of Evaluation of drug activity against intracellular forms of Trypanosoma cruzi employing enzyme immunoassay" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Evaluation of drug activity against intracellular forms of Trypanosoma cruzi employing enzyme immunoassay</div><div class="wp-workCard_item"><span>Journal of Clinical Pharmacy and Therapeutics</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">To describe application of a new method for the evaluation of anti-Trypanosoma cruzi activity aga...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">To describe application of a new method for the evaluation of anti-Trypanosoma cruzi activity against intracellular forms. Vero fibroblasts in 96-well tissue culture plates were infected with trypomastigote forms of T. cruzi. Amastigotes growth was estimated after 24 and 96 h both by microscopic counts of Giemsa-stained monolayers and enzyme-linked immunoIsorbent assay (ELISA). ELISA was performed directly on the fixed cultures using a rabbit anti-T. cruzi immunoIglobulin as the first antibody and a peroxidase-labelled antirabbit immunoglobulin as the second antibody. Three chemical series of structural analogous of gentian violet, thiadiazines and derivatives of 5-nitrothiophene-2-carbaldehyde as well as three reference compounds (nifurtimox, benznidazole and gentian violet) were then assayed. The anti-T. cruzi activity of all of them had been determined previously by microscopic counting of Giemsa-stained infected cultures. None of the assayed compounds showed better activity than the reference ones, but the application of the enzyme immunoassay to quantify the inhibition of growth amastigotes is of great interest, as it yielded results comparable with microscopic counts. ELISA can be applied to pharmacological screening, with some advantages over the microscopic examination, including possible automation, rapidity and objectivity in assessment.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579209"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579209"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579209; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579209]").text(description); $(".js-view-count[data-work-id=111579209]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579209; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579209']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=111579209]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579209,"title":"Evaluation of drug activity against intracellular forms of Trypanosoma cruzi employing enzyme immunoassay","translated_title":"","metadata":{"abstract":"To describe application of a new method for the evaluation of anti-Trypanosoma cruzi activity against intracellular forms. Vero fibroblasts in 96-well tissue culture plates were infected with trypomastigote forms of T. cruzi. Amastigotes growth was estimated after 24 and 96 h both by microscopic counts of Giemsa-stained monolayers and enzyme-linked immunoIsorbent assay (ELISA). ELISA was performed directly on the fixed cultures using a rabbit anti-T. cruzi immunoIglobulin as the first antibody and a peroxidase-labelled antirabbit immunoglobulin as the second antibody. Three chemical series of structural analogous of gentian violet, thiadiazines and derivatives of 5-nitrothiophene-2-carbaldehyde as well as three reference compounds (nifurtimox, benznidazole and gentian violet) were then assayed. The anti-T. cruzi activity of all of them had been determined previously by microscopic counting of Giemsa-stained infected cultures. None of the assayed compounds showed better activity than the reference ones, but the application of the enzyme immunoassay to quantify the inhibition of growth amastigotes is of great interest, as it yielded results comparable with microscopic counts. ELISA can be applied to pharmacological screening, with some advantages over the microscopic examination, including possible automation, rapidity and objectivity in assessment.","publisher":"Wiley-Blackwell","publication_date":{"day":null,"month":null,"year":2000,"errors":{}},"publication_name":"Journal of Clinical Pharmacy and Therapeutics"},"translated_abstract":"To describe application of a new method for the evaluation of anti-Trypanosoma cruzi activity against intracellular forms. Vero fibroblasts in 96-well tissue culture plates were infected with trypomastigote forms of T. cruzi. Amastigotes growth was estimated after 24 and 96 h both by microscopic counts of Giemsa-stained monolayers and enzyme-linked immunoIsorbent assay (ELISA). ELISA was performed directly on the fixed cultures using a rabbit anti-T. cruzi immunoIglobulin as the first antibody and a peroxidase-labelled antirabbit immunoglobulin as the second antibody. Three chemical series of structural analogous of gentian violet, thiadiazines and derivatives of 5-nitrothiophene-2-carbaldehyde as well as three reference compounds (nifurtimox, benznidazole and gentian violet) were then assayed. The anti-T. cruzi activity of all of them had been determined previously by microscopic counting of Giemsa-stained infected cultures. None of the assayed compounds showed better activity than the reference ones, but the application of the enzyme immunoassay to quantify the inhibition of growth amastigotes is of great interest, as it yielded results comparable with microscopic counts. ELISA can be applied to pharmacological screening, with some advantages over the microscopic examination, including possible automation, rapidity and objectivity in assessment.","internal_url":"https://www.academia.edu/111579209/Evaluation_of_drug_activity_against_intracellular_forms_of_Trypanosoma_cruzi_employing_enzyme_immunoassay","translated_internal_url":"","created_at":"2023-12-16T10:32:54.471-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Evaluation_of_drug_activity_against_intracellular_forms_of_Trypanosoma_cruzi_employing_enzyme_immunoassay","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"To describe application of a new method for the evaluation of anti-Trypanosoma cruzi activity against intracellular forms. Vero fibroblasts in 96-well tissue culture plates were infected with trypomastigote forms of T. cruzi. Amastigotes growth was estimated after 24 and 96 h both by microscopic counts of Giemsa-stained monolayers and enzyme-linked immunoIsorbent assay (ELISA). ELISA was performed directly on the fixed cultures using a rabbit anti-T. cruzi immunoIglobulin as the first antibody and a peroxidase-labelled antirabbit immunoglobulin as the second antibody. Three chemical series of structural analogous of gentian violet, thiadiazines and derivatives of 5-nitrothiophene-2-carbaldehyde as well as three reference compounds (nifurtimox, benznidazole and gentian violet) were then assayed. The anti-T. cruzi activity of all of them had been determined previously by microscopic counting of Giemsa-stained infected cultures. None of the assayed compounds showed better activity than the reference ones, but the application of the enzyme immunoassay to quantify the inhibition of growth amastigotes is of great interest, as it yielded results comparable with microscopic counts. ELISA can be applied to pharmacological screening, with some advantages over the microscopic examination, including possible automation, rapidity and objectivity in assessment.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":99708,"name":"Clinical","url":"https://www.academia.edu/Documents/in/Clinical"},{"id":111012,"name":"Plant tissue Culture Techniques","url":"https://www.academia.edu/Documents/in/Plant_tissue_Culture_Techniques"},{"id":225340,"name":"Chagas disease","url":"https://www.academia.edu/Documents/in/Chagas_disease"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":511535,"name":"Immunoassay","url":"https://www.academia.edu/Documents/in/Immunoassay"},{"id":619383,"name":"Benznidazole","url":"https://www.academia.edu/Documents/in/Benznidazole"},{"id":619386,"name":"Nifurtimox","url":"https://www.academia.edu/Documents/in/Nifurtimox"},{"id":744836,"name":"Trypanocidal Agents","url":"https://www.academia.edu/Documents/in/Trypanocidal_Agents"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1312086,"name":"Immunoglobulins","url":"https://www.academia.edu/Documents/in/Immunoglobulins"},{"id":2032101,"name":"Intracellular","url":"https://www.academia.edu/Documents/in/Intracellular"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"},{"id":3948095,"name":" Giemsa stain","url":"https://www.academia.edu/Documents/in/Giemsa_stain"}],"urls":[{"id":37206615,"url":"https://doi.org/10.1046/j.1365-2710.2000.00256.x"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579207"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579207/A_new_pharmacological_screening_assay_with_Trypanosoma_cruzi_epimastigotes_expressing_galactosidase"><img alt="Research paper thumbnail of A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing ?-galactosidase" class="work-thumbnail" src="https://attachments.academia-assets.com/109077011/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579207/A_new_pharmacological_screening_assay_with_Trypanosoma_cruzi_epimastigotes_expressing_galactosidase">A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing ?-galactosidase</a></div><div class="wp-workCard_item"><span>Parasitology Research</span><span>, Jan 29, 2005</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (c...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic b-galactosidase released into the culture on membrane lysis in the presence of the substrate chlorophenol red b-D-galactopyranoside (CPRG). The experimental conditions were adjusted to find those in which the relationship between epimastigote number and CPRG absorbance was linear over the widest possible range. Absorbance was significantly correlated with the number epimastigote from 5•10 3 to 1.2•10 6 parasites/ml (r=0.98, P<0.01). The optimal final concentration of CPRG was 200 lM and the optimal incubation period was 6 h when parasites were incubated for 3 days. Once the assay was standardized, the trypanocidal activities of nifurtimox and benznidazole were determined both by CPRG assay and microscopic counting, demonstrating the methods utility for drugscreening. The efficacy obtained was comparable to that obtained with the manual method.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fd5a683fb435cef90567f0b96dc1d92e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077011,"asset_id":111579207,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077011/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579207"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579207"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579207; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579207]").text(description); $(".js-view-count[data-work-id=111579207]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579207; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579207']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fd5a683fb435cef90567f0b96dc1d92e" } } $('.js-work-strip[data-work-id=111579207]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579207,"title":"A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing ?-galactosidase","translated_title":"","metadata":{"publisher":"Springer Science+Business Media","grobid_abstract":"We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic b-galactosidase released into the culture on membrane lysis in the presence of the substrate chlorophenol red b-D-galactopyranoside (CPRG). The experimental conditions were adjusted to find those in which the relationship between epimastigote number and CPRG absorbance was linear over the widest possible range. Absorbance was significantly correlated with the number epimastigote from 5•10 3 to 1.2•10 6 parasites/ml (r=0.98, P\u003c0.01). The optimal final concentration of CPRG was 200 lM and the optimal incubation period was 6 h when parasites were incubated for 3 days. Once the assay was standardized, the trypanocidal activities of nifurtimox and benznidazole were determined both by CPRG assay and microscopic counting, demonstrating the methods utility for drugscreening. The efficacy obtained was comparable to that obtained with the manual method.","publication_date":{"day":29,"month":1,"year":2005,"errors":{}},"publication_name":"Parasitology Research","grobid_abstract_attachment_id":109077011},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579207/A_new_pharmacological_screening_assay_with_Trypanosoma_cruzi_epimastigotes_expressing_galactosidase","translated_internal_url":"","created_at":"2023-12-16T10:32:53.973-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109077011,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077011/thumbnails/1.jpg","file_name":"s00436-005-1300-320231216-1-awp2zu.pdf","download_url":"https://www.academia.edu/attachments/109077011/download_file","bulk_download_file_name":"A_new_pharmacological_screening_assay_wi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077011/s00436-005-1300-320231216-1-awp2zu-libre.pdf?1702752314=\u0026response-content-disposition=attachment%3B+filename%3DA_new_pharmacological_screening_assay_wi.pdf\u0026Expires=1743089736\u0026Signature=VPQ72d5ijJJXn9nT8nbYuKV2Gk1lEURc3iw2DvPpb5WjY7o14aGrEKFBABSv4jzX~KZEDvZTpxjCMfdIB1lqwnVYgnqUi5MOYUV92fq5LoBd35czcKH4u2p3lvf~gEKpsWdsoTAS6WHTw5djYkCnDbZS0JMwyJluzl~6nTGziMk4DWTd6mK1QLoyZtIhA8iFKF3YVfq5FM-XXmXTGKmTs8g67v-C40XYJJSaEebMtp474229YMpEn3nmRXeBbpzb-5TaoYBaBzJ6mdVBVQdZ1tmXTYmB1OaQAU-oqw1jVT5vQdVUZLl9AnCycwEZP5t8JM3zaTRvh1JHQqDGCsSDtw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_new_pharmacological_screening_assay_with_Trypanosoma_cruzi_epimastigotes_expressing_galactosidase","translated_slug":"","page_count":3,"language":"en","content_type":"Work","summary":"We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic b-galactosidase released into the culture on membrane lysis in the presence of the substrate chlorophenol red b-D-galactopyranoside (CPRG). The experimental conditions were adjusted to find those in which the relationship between epimastigote number and CPRG absorbance was linear over the widest possible range. Absorbance was significantly correlated with the number epimastigote from 5•10 3 to 1.2•10 6 parasites/ml (r=0.98, P\u003c0.01). The optimal final concentration of CPRG was 200 lM and the optimal incubation period was 6 h when parasites were incubated for 3 days. Once the assay was standardized, the trypanocidal activities of nifurtimox and benznidazole were determined both by CPRG assay and microscopic counting, demonstrating the methods utility for drugscreening. The efficacy obtained was comparable to that obtained with the manual method.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109077011,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077011/thumbnails/1.jpg","file_name":"s00436-005-1300-320231216-1-awp2zu.pdf","download_url":"https://www.academia.edu/attachments/109077011/download_file","bulk_download_file_name":"A_new_pharmacological_screening_assay_wi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077011/s00436-005-1300-320231216-1-awp2zu-libre.pdf?1702752314=\u0026response-content-disposition=attachment%3B+filename%3DA_new_pharmacological_screening_assay_wi.pdf\u0026Expires=1743089736\u0026Signature=VPQ72d5ijJJXn9nT8nbYuKV2Gk1lEURc3iw2DvPpb5WjY7o14aGrEKFBABSv4jzX~KZEDvZTpxjCMfdIB1lqwnVYgnqUi5MOYUV92fq5LoBd35czcKH4u2p3lvf~gEKpsWdsoTAS6WHTw5djYkCnDbZS0JMwyJluzl~6nTGziMk4DWTd6mK1QLoyZtIhA8iFKF3YVfq5FM-XXmXTGKmTs8g67v-C40XYJJSaEebMtp474229YMpEn3nmRXeBbpzb-5TaoYBaBzJ6mdVBVQdZ1tmXTYmB1OaQAU-oqw1jVT5vQdVUZLl9AnCycwEZP5t8JM3zaTRvh1JHQqDGCsSDtw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":164,"name":"Parasitology","url":"https://www.academia.edu/Documents/in/Parasitology"},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli"},{"id":122642,"name":"Drug Screening","url":"https://www.academia.edu/Documents/in/Drug_Screening"},{"id":178351,"name":"Spectrophotometry","url":"https://www.academia.edu/Documents/in/Spectrophotometry"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":619383,"name":"Benznidazole","url":"https://www.academia.edu/Documents/in/Benznidazole"},{"id":619386,"name":"Nifurtimox","url":"https://www.academia.edu/Documents/in/Nifurtimox"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":744836,"name":"Trypanocidal Agents","url":"https://www.academia.edu/Documents/in/Trypanocidal_Agents"},{"id":3557974,"name":"Chlorophenols","url":"https://www.academia.edu/Documents/in/Chlorophenols"}],"urls":[{"id":37206613,"url":"https://doi.org/10.1007/s00436-005-1300-3"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579205"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579205/Biological_characterization_of_Trypanosoma_cruzi_strains"><img alt="Research paper thumbnail of Biological characterization of Trypanosoma cruzi strains" class="work-thumbnail" src="https://attachments.academia-assets.com/109076954/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579205/Biological_characterization_of_Trypanosoma_cruzi_strains">Biological characterization of Trypanosoma cruzi strains</a></div><div class="wp-workCard_item"><span>Memorias Do Instituto Oswaldo Cruz</span><span>, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Biological parameters of five Trypanosoma cruzi strains from different sources were determined in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Biological parameters of five Trypanosoma cruzi strains from different sources were determined in order to know the laboratory behaviour of natural populations. The parameters evaluated were growth kinetics of epimastigotes, differentiation into metacyclic forms, infectivity in mammalian cells grown in vitro and parasite susceptibility to nifurtimox, benznidazole and gentian violet. Differences in transformation to metacyclic, in the percentage of infected cells as well as in the number of amastigotes per cell were observed among the strains. Regarding to pharmacological assays, Y strain was the most sensitive to the three assayed compounds. These data demonstrate the heterogeneity of natural populations of T. cruzi, the only responsible of infection in humans.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="93866e5b4cf72233ab5d29b1097fb71f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076954,"asset_id":111579205,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076954/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579205"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579205"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579205; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579205]").text(description); $(".js-view-count[data-work-id=111579205]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579205; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579205']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "93866e5b4cf72233ab5d29b1097fb71f" } } $('.js-work-strip[data-work-id=111579205]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579205,"title":"Biological characterization of Trypanosoma cruzi strains","translated_title":"","metadata":{"publisher":"Instituto Oswaldo Cruz, Ministério da Saúde","grobid_abstract":"Biological parameters of five Trypanosoma cruzi strains from different sources were determined in order to know the laboratory behaviour of natural populations. The parameters evaluated were growth kinetics of epimastigotes, differentiation into metacyclic forms, infectivity in mammalian cells grown in vitro and parasite susceptibility to nifurtimox, benznidazole and gentian violet. Differences in transformation to metacyclic, in the percentage of infected cells as well as in the number of amastigotes per cell were observed among the strains. Regarding to pharmacological assays, Y strain was the most sensitive to the three assayed compounds. 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Gross histopathologic changes in abdominal organs and mortality rate, as a consequence of the pathogenicity of the protozoa and the immune response of the host, were evaluated. Among the different treatment regimes assayed, Anapsos® at doses of 20 mg/Kg/day administered for 1 0 days before infection decreases the parasite pathogenicity index (PI) in the treated animals when compared to those of the untreated control group. The immunosuppresor treatments with azathioprine (100 mg/Kg/day x 1), cyclophosphamide (100 mg/Kg/day x 1), and FK-506 (10 mg/Kg/day x 10) significantly decreased the PI, while an immunostimulant treatment with glycophosphopeptical (13 mg/Kg/day x 10) increased it. These assays have shown the usefulness of the murine model of experimental trichomoniasis for the study of immunomodulator activity of natural or synthetic drugs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f0e5fdcf7e2c3d5be8a231ebe4a98cec" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077027,"asset_id":111579200,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077027/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579200"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579200"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579200; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579200]").text(description); $(".js-view-count[data-work-id=111579200]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579200; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579200']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f0e5fdcf7e2c3d5be8a231ebe4a98cec" } } $('.js-work-strip[data-work-id=111579200]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579200,"title":"Effect of Anapsos® in a murine model of experimental trichomoniasis","translated_title":"","metadata":{"publisher":"EDP Sciences","grobid_abstract":"Immunomodulator effect of Anapsos® (Polypodium leucotomos extract) in NMRI (US Naval Medical Research Institute) outbred mice infected by the intraperitoneal route with 10 7 Trichomonas vaginalis has been tested. Gross histopathologic changes in abdominal organs and mortality rate, as a consequence of the pathogenicity of the protozoa and the immune response of the host, were evaluated. Among the different treatment regimes assayed, Anapsos® at doses of 20 mg/Kg/day administered for 1 0 days before infection decreases the parasite pathogenicity index (PI) in the treated animals when compared to those of the untreated control group. The immunosuppresor treatments with azathioprine (100 mg/Kg/day x 1), cyclophosphamide (100 mg/Kg/day x 1), and FK-506 (10 mg/Kg/day x 10) significantly decreased the PI, while an immunostimulant treatment with glycophosphopeptical (13 mg/Kg/day x 10) increased it. 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Gross histopathologic changes in abdominal organs and mortality rate, as a consequence of the pathogenicity of the protozoa and the immune response of the host, were evaluated. Among the different treatment regimes assayed, Anapsos® at doses of 20 mg/Kg/day administered for 1 0 days before infection decreases the parasite pathogenicity index (PI) in the treated animals when compared to those of the untreated control group. The immunosuppresor treatments with azathioprine (100 mg/Kg/day x 1), cyclophosphamide (100 mg/Kg/day x 1), and FK-506 (10 mg/Kg/day x 10) significantly decreased the PI, while an immunostimulant treatment with glycophosphopeptical (13 mg/Kg/day x 10) increased it. These assays have shown the usefulness of the murine model of experimental trichomoniasis for the study of immunomodulator activity of natural or synthetic drugs.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109077027,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077027/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/109077027/download_file","bulk_download_file_name":"Effect_of_Anapsos_in_a_murine_model_of_e.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077027/pdf-libre.pdf?1702752323=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_Anapsos_in_a_murine_model_of_e.pdf\u0026Expires=1743089736\u0026Signature=FFPCKs6yJKwAqrIzmxBVUvBnQDDkCZ0Y81QqZyLIB7V85FwZpHSWaHtUGSEZEpAH-MCu0919W0MeFtcbuTrQfepVH53hLJg~JTWK8fUyfW2HAApl6yNm0OYVJnhYMRSKFlsHYWyaFQ1J1Hh7FgxVECygo-Z8Lmtk8XaFn20a~0yv2CtpwLTUkHmuA6~7f8vr2hhHkkXV-UT6ZgCWGgruxeHUUiBYr-tFMKmA8PRQxdrSIFYXDb-KNvYT27J0mSdT4fAjdF8WRFitbBJpBnRvf0CoI7UXXlksEfm0v9uDnARs2tDXDiTOswT1x0rJSehzxfEQFkLyHGEboKwUElNgaQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":29974,"name":"Disease susceptibility","url":"https://www.academia.edu/Documents/in/Disease_susceptibility"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":94271,"name":"Parasite","url":"https://www.academia.edu/Documents/in/Parasite"},{"id":143830,"name":"Immunomodulation","url":"https://www.academia.edu/Documents/in/Immunomodulation"},{"id":324154,"name":"Immune system","url":"https://www.academia.edu/Documents/in/Immune_system"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts"},{"id":770335,"name":"Immunostimulant","url":"https://www.academia.edu/Documents/in/Immunostimulant"},{"id":1198586,"name":"FK","url":"https://www.academia.edu/Documents/in/FK"},{"id":1198588,"name":"Cyclophosphamide","url":"https://www.academia.edu/Documents/in/Cyclophosphamide"},{"id":1533653,"name":"Glycosides","url":"https://www.academia.edu/Documents/in/Glycosides"},{"id":1740531,"name":"Azathioprine","url":"https://www.academia.edu/Documents/in/Azathioprine"},{"id":2463495,"name":"Random Allocation","url":"https://www.academia.edu/Documents/in/Random_Allocation"},{"id":2562571,"name":"Immunosuppressive Agents","url":"https://www.academia.edu/Documents/in/Immunosuppressive_Agents"}],"urls":[{"id":37206608,"url":"https://doi.org/10.1051/parasite/2003104303"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579199"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579199/Evaluation_of_a_murine_model_of_experimental_trichomoniasis"><img alt="Research paper thumbnail of Evaluation of a murine model of experimental trichomoniasis" class="work-thumbnail" src="https://attachments.academia-assets.com/109077024/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579199/Evaluation_of_a_murine_model_of_experimental_trichomoniasis">Evaluation of a murine model of experimental trichomoniasis</a></div><div class="wp-workCard_item"><span>Parasite</span><span>, Jun 1, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">By using a reference strain of Trichomonas vaginalis and the intraperitoneal route for infecting ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">By using a reference strain of Trichomonas vaginalis and the intraperitoneal route for infecting animals, the influence of the strain of mice, the time observation and the inoculation doses were followed in order to standardize the optimal conditions for the evolution of experimental trichomoniasis. Our tesults suggest that the inoculation of BALB/c mice with 10 7 trichomonads and the semiquantitative assessment at day 15 postinfection of the grosspathologic changes in the abdominal cavity-peritoneum, spleen, pancreas, stomach and liver-as well as the presence of ascitic fluid and mortality, maybe a suitable laboratory model of trichomoniasis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="74f6559607afe689f9759207c788e73d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077024,"asset_id":111579199,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077024/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579199"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579199"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579199; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579199]").text(description); $(".js-view-count[data-work-id=111579199]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579199; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579199']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "74f6559607afe689f9759207c788e73d" } } $('.js-work-strip[data-work-id=111579199]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579199,"title":"Evaluation of a murine model of experimental trichomoniasis","translated_title":"","metadata":{"publisher":"EDP Sciences","grobid_abstract":"By using a reference strain of Trichomonas vaginalis and the intraperitoneal route for infecting animals, the influence of the strain of mice, the time observation and the inoculation doses were followed in order to standardize the optimal conditions for the evolution of experimental trichomoniasis. Our tesults suggest that the inoculation of BALB/c mice with 10 7 trichomonads and the semiquantitative assessment at day 15 postinfection of the grosspathologic changes in the abdominal cavity-peritoneum, spleen, pancreas, stomach and liver-as well as the presence of ascitic fluid and mortality, maybe a suitable laboratory model of trichomoniasis.","publication_date":{"day":1,"month":6,"year":1997,"errors":{}},"publication_name":"Parasite","grobid_abstract_attachment_id":109077024},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579199/Evaluation_of_a_murine_model_of_experimental_trichomoniasis","translated_internal_url":"","created_at":"2023-12-16T10:32:52.179-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109077024,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109077024/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/109077024/download_file","bulk_download_file_name":"Evaluation_of_a_murine_model_of_experime.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109077024/pdf-libre.pdf?1702752320=\u0026response-content-disposition=attachment%3B+filename%3DEvaluation_of_a_murine_model_of_experime.pdf\u0026Expires=1743089736\u0026Signature=IZuZD5akzjbbDia9zfXTF3gIkmtkcBK1OSK8UzRUpK1Js7WQtkAv4Yg6pyQOx~T~PSP6yC-fitS2wBl3DRbgXe4XV-gUG~jDxNTUPIGonLZvS3ppfPM-FUbO75VhXe2OlUqQt5iQkCF599QJ2lu2LSBC~-NaX1Ci0mma-2Grus2HRWAo6S4zDWFcB9Me9ZJ4wMuz~skgDOSW53afpFd2bgQdvuHconJD5wVjBNuzUmc7kt9Ib4THn06s6RZr1umoZwE~EI97RTssU8R-GbCuC5JyqQsMnudr0Rt6JDW2ooTL4zzk3j39MSogBSRw9ZaCZpjLa6kbthhEQzEmDJekJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Evaluation_of_a_murine_model_of_experimental_trichomoniasis","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"By using a reference strain of Trichomonas vaginalis and the intraperitoneal route for infecting animals, the influence of the strain of mice, the time observation and the inoculation doses were followed in order to standardize the optimal conditions for the evolution of experimental trichomoniasis. 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A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cce8d8faa4df75bde894412749ada07b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076951,"asset_id":111579196,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076951/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579196"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579196"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579196; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579196]").text(description); $(".js-view-count[data-work-id=111579196]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579196; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579196']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cce8d8faa4df75bde894412749ada07b" } } $('.js-work-strip[data-work-id=111579196]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579196,"title":"Biological variability in clinical isolates of Trichomonas vaginalis","translated_title":"","metadata":{"publisher":"Instituto Oswaldo Cruz, Ministério da Saúde","grobid_abstract":"Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Goverment of Madrid. A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.","publication_date":{"day":1,"month":9,"year":2002,"errors":{}},"publication_name":"Memorias Do Instituto Oswaldo Cruz","grobid_abstract_attachment_id":109076951},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579196/Biological_variability_in_clinical_isolates_of_Trichomonas_vaginalis","translated_internal_url":"","created_at":"2023-12-16T10:32:51.582-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109076951,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076951/thumbnails/1.jpg","file_name":"FVHzmz6DDF3BXH9cjyQSyhD.pdf","download_url":"https://www.academia.edu/attachments/109076951/download_file","bulk_download_file_name":"Biological_variability_in_clinical_isola.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076951/FVHzmz6DDF3BXH9cjyQSyhD-libre.pdf?1702752319=\u0026response-content-disposition=attachment%3B+filename%3DBiological_variability_in_clinical_isola.pdf\u0026Expires=1743089736\u0026Signature=CSuSQmSYFIa0hNDWDG9OH4jJdh0OrClgqMwzVSzA~dUsyJ5lHtEW6lRZcNJScDvjDv42bYhxKWpLtfj9q3QnT59gb3P5FM-Nk9aGzcy~tLHuSNuXKT7PAFlc2eH3198Qe3IMOglgaXLJTtwQBMr~bUVMviNmqZyMIYpnmrRn3O5kVt7-axFsxhhH99v2WCIpTOoqY288R9j5V5NtRUuyCNOZ5PF9prUYCRelfL6vypgQkFywOKtfNBm5-3kNXooWx4b6eYZ~4EK~uMlRLBgFxxuTnrMIyR8BaSmHL7HawVfGhLB3aN6H2uQJYJeP-OAbNJZEKrVrinIsf7ouojuVwA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Biological_variability_in_clinical_isolates_of_Trichomonas_vaginalis","translated_slug":"","page_count":4,"language":"en","content_type":"Work","summary":"Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Goverment of Madrid. A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109076951,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076951/thumbnails/1.jpg","file_name":"FVHzmz6DDF3BXH9cjyQSyhD.pdf","download_url":"https://www.academia.edu/attachments/109076951/download_file","bulk_download_file_name":"Biological_variability_in_clinical_isola.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076951/FVHzmz6DDF3BXH9cjyQSyhD-libre.pdf?1702752319=\u0026response-content-disposition=attachment%3B+filename%3DBiological_variability_in_clinical_isola.pdf\u0026Expires=1743089737\u0026Signature=TyWvujzaGo~yiy9mmL-l9K6TIA4bW2NJ5toXZfjTxMtmxpExA1ySfN5yZPQmYQiV~P0wIs1PfMvkUnp-7p7~43cwVCY~4Cx3YIWw6fFak27Mckn4g~CYfVTfd-6nL2lLkfpeyv-SWty-p-wh5HllgftBhILqjx2SbSU8plv1atc4tjRvb3sKN0YWXbqmRO9xYzunbPyZRliIXOGXZOvuezxpepfe7GcIAZDv3sokD3soHkH5KgIrrKBQ~kTGC~gNvPZbTmVKkbilVcUzgj6st5ow9Eej9rOUvFyYzkFAZNgNrfmSRKIsEJQbMiKMyVOucla8aX8TYSSd9yntcSMECg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":52873,"name":"Virulence","url":"https://www.academia.edu/Documents/in/Virulence"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":413804,"name":"Inoculation","url":"https://www.academia.edu/Documents/in/Inoculation"},{"id":957793,"name":"Pathogenicity","url":"https://www.academia.edu/Documents/in/Pathogenicity"},{"id":1303572,"name":"Metronidazole","url":"https://www.academia.edu/Documents/in/Metronidazole"},{"id":4063964,"name":"Trichomonas","url":"https://www.academia.edu/Documents/in/Trichomonas"}],"urls":[{"id":37206605,"url":"https://www.scielo.br/j/mioc/a/FVHzmz6DDF3BXH9cjyQSyhD/?lang=en\u0026format=pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579194"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/111579194/Activity_Assays_of_Thiadiazine_Derivatives_on_and_lt_i_and_gt_Trichomonas_vaginalis_and_lt_i_and_gt_and_Amastigote_Forms_of_and_lt_i_and_gt_Trypanosoma_cruzi_and_lt_i_and_gt"><img alt="Research paper thumbnail of Activity Assays of Thiadiazine Derivatives on &lt;i&gt;Trichomonas vaginalis &lt;/i&gt;and Amastigote Forms of &lt;i&gt;Trypanosoma cruzi&lt;/i&gt" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Activity Assays of Thiadiazine Derivatives on &lt;i&gt;Trichomonas vaginalis &lt;/i&gt;and Amastigote Forms of &lt;i&gt;Trypanosoma cruzi&lt;/i&gt</div><div class="wp-workCard_item"><span>Chemotherapy</span><span>, 1992</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. In vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. The capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. One of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronidazole, although higher doses were required. Its toxicity on cell proliferation in tissue culture was moderate. In vitro assays on amastigote forms of Trypanosoma cruzi were carried out using cultures of Vero cells infected with metacyclic trypomastigotes. For one compound (1) trypanocidal activity resembled that of nifurtimox as assessed by microscopic counts of infected and uninfected cells. Unfortunately, this compound showed a high degree of cytotoxicity on Vero cell cultures.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579194"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579194"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579194; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579194]").text(description); $(".js-view-count[data-work-id=111579194]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579194; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579194']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=111579194]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579194,"title":"Activity Assays of Thiadiazine Derivatives on \u0026lt;i\u0026gt;Trichomonas vaginalis \u0026lt;/i\u0026gt;and Amastigote Forms of \u0026lt;i\u0026gt;Trypanosoma cruzi\u0026lt;/i\u0026gt","translated_title":"","metadata":{"abstract":"Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. In vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. The capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. One of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronidazole, although higher doses were required. Its toxicity on cell proliferation in tissue culture was moderate. In vitro assays on amastigote forms of Trypanosoma cruzi were carried out using cultures of Vero cells infected with metacyclic trypomastigotes. For one compound (1) trypanocidal activity resembled that of nifurtimox as assessed by microscopic counts of infected and uninfected cells. Unfortunately, this compound showed a high degree of cytotoxicity on Vero cell cultures.","publisher":"Karger Publishers","publication_date":{"day":null,"month":null,"year":1992,"errors":{}},"publication_name":"Chemotherapy"},"translated_abstract":"Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. In vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. The capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. One of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronidazole, although higher doses were required. Its toxicity on cell proliferation in tissue culture was moderate. In vitro assays on amastigote forms of Trypanosoma cruzi were carried out using cultures of Vero cells infected with metacyclic trypomastigotes. For one compound (1) trypanocidal activity resembled that of nifurtimox as assessed by microscopic counts of infected and uninfected cells. Unfortunately, this compound showed a high degree of cytotoxicity on Vero cell cultures.","internal_url":"https://www.academia.edu/111579194/Activity_Assays_of_Thiadiazine_Derivatives_on_and_lt_i_and_gt_Trichomonas_vaginalis_and_lt_i_and_gt_and_Amastigote_Forms_of_and_lt_i_and_gt_Trypanosoma_cruzi_and_lt_i_and_gt","translated_internal_url":"","created_at":"2023-12-16T10:32:50.998-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Activity_Assays_of_Thiadiazine_Derivatives_on_and_lt_i_and_gt_Trichomonas_vaginalis_and_lt_i_and_gt_and_Amastigote_Forms_of_and_lt_i_and_gt_Trypanosoma_cruzi_and_lt_i_and_gt","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. In vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. The capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. One of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronidazole, although higher doses were required. Its toxicity on cell proliferation in tissue culture was moderate. In vitro assays on amastigote forms of Trypanosoma cruzi were carried out using cultures of Vero cells infected with metacyclic trypomastigotes. For one compound (1) trypanocidal activity resembled that of nifurtimox as assessed by microscopic counts of infected and uninfected cells. Unfortunately, this compound showed a high degree of cytotoxicity on Vero cell cultures.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":9114,"name":"Chemotherapy","url":"https://www.academia.edu/Documents/in/Chemotherapy"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":37782,"name":"Cell Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro"},{"id":66089,"name":"Tissue culture","url":"https://www.academia.edu/Documents/in/Tissue_culture"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":238630,"name":"Experimental Infection","url":"https://www.academia.edu/Documents/in/Experimental_Infection"},{"id":302037,"name":"In Vivo","url":"https://www.academia.edu/Documents/in/In_Vivo"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":619383,"name":"Benznidazole","url":"https://www.academia.edu/Documents/in/Benznidazole"},{"id":619386,"name":"Nifurtimox","url":"https://www.academia.edu/Documents/in/Nifurtimox"},{"id":744836,"name":"Trypanocidal Agents","url":"https://www.academia.edu/Documents/in/Trypanocidal_Agents"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation"},{"id":951639,"name":"Murine Model","url":"https://www.academia.edu/Documents/in/Murine_Model"},{"id":1303572,"name":"Metronidazole","url":"https://www.academia.edu/Documents/in/Metronidazole"},{"id":2469399,"name":"Thiadiazines","url":"https://www.academia.edu/Documents/in/Thiadiazines"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[{"id":37206603,"url":"https://doi.org/10.1159/000239040"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579190"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579190/Modulation_byPolypodium_leucotomosextract_of_cytokine_patterns_in_experimental_trichomoniasis_model"><img alt="Research paper thumbnail of Modulation byPolypodium leucotomosextract of cytokine patterns in experimental trichomoniasis model" class="work-thumbnail" src="https://attachments.academia-assets.com/109076950/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579190/Modulation_byPolypodium_leucotomosextract_of_cytokine_patterns_in_experimental_trichomoniasis_model">Modulation byPolypodium leucotomosextract of cytokine patterns in experimental trichomoniasis model</a></div><div class="wp-workCard_item"><span>Parasite</span><span>, Mar 1, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The immunomodulating effects of Anapsos®, an aqueous hydrosoluble extract obtained from the rhizo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The immunomodulating effects of Anapsos®, an aqueous hydrosoluble extract obtained from the rhizomes of the fern Polypodium leucotomos, on both pathogenicity and cytokine levels in serum (IFN-y/IL-4) were assayed in a Trichomonas vaginalis experimental model (BALB/c mice infected with 10 7 trichomonads and examined at day 15 after infection). Doses of 20 mg/kg/day administered for 10 days before the infection with the parasite induced a decrease of the experimental pathogenicity approximately 1 0-20 % compared to controls. Gross histopathologic changes at abdominal organs and mortality rale, as a consequence of pathogenicity of the protozoa and the immune response of the host, were evaluated. IFN-y and IL-4 cytokines were determined on days-5, 0, 5, 10, and 15 postinfection by indirect ELISA. Treatment with PAL before infection modulates and downregulates the IFN-y concentration, while anticipates and upregulates the IL-4 level. The assays performed have showed the utility of the murine model of experimental trichomoniasis for the evaluation of immunomodulatory activity of synthetic or natural products.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b779e431b32ebd6e47db17262a66be84" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076950,"asset_id":111579190,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076950/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579190"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579190"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579190; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579190]").text(description); $(".js-view-count[data-work-id=111579190]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579190; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579190']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b779e431b32ebd6e47db17262a66be84" } } $('.js-work-strip[data-work-id=111579190]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579190,"title":"Modulation byPolypodium leucotomosextract of cytokine patterns in experimental trichomoniasis model","translated_title":"","metadata":{"publisher":"EDP Sciences","ai_title_tag":"Polypodium leucotomos Extract in Trichomoniasis","grobid_abstract":"The immunomodulating effects of Anapsos®, an aqueous hydrosoluble extract obtained from the rhizomes of the fern Polypodium leucotomos, on both pathogenicity and cytokine levels in serum (IFN-y/IL-4) were assayed in a Trichomonas vaginalis experimental model (BALB/c mice infected with 10 7 trichomonads and examined at day 15 after infection). Doses of 20 mg/kg/day administered for 10 days before the infection with the parasite induced a decrease of the experimental pathogenicity approximately 1 0-20 % compared to controls. Gross histopathologic changes at abdominal organs and mortality rale, as a consequence of pathogenicity of the protozoa and the immune response of the host, were evaluated. IFN-y and IL-4 cytokines were determined on days-5, 0, 5, 10, and 15 postinfection by indirect ELISA. Treatment with PAL before infection modulates and downregulates the IFN-y concentration, while anticipates and upregulates the IL-4 level. The assays performed have showed the utility of the murine model of experimental trichomoniasis for the evaluation of immunomodulatory activity of synthetic or natural products.","publication_date":{"day":1,"month":3,"year":2003,"errors":{}},"publication_name":"Parasite","grobid_abstract_attachment_id":109076950},"translated_abstract":null,"internal_url":"https://www.academia.edu/111579190/Modulation_byPolypodium_leucotomosextract_of_cytokine_patterns_in_experimental_trichomoniasis_model","translated_internal_url":"","created_at":"2023-12-16T10:32:50.513-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":109076950,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076950/thumbnails/1.jpg","file_name":"parasite2003101p73.pdf","download_url":"https://www.academia.edu/attachments/109076950/download_file","bulk_download_file_name":"Modulation_byPolypodium_leucotomosextrac.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076950/parasite2003101p73-libre.pdf?1702752325=\u0026response-content-disposition=attachment%3B+filename%3DModulation_byPolypodium_leucotomosextrac.pdf\u0026Expires=1743089737\u0026Signature=CsDKmwcKzim8c-qB1EdTgdZhAQlT1LTnTkKqPLyDQP820zACoXnl3~DR1sw9lshJ33oUe6gHqdfwdwpZzhOMqi0pf3aJ1HPy7cCSexm0OZ8YabiLKIrdyLXri1ZUM74aF~6Odt1qniRZkfpQ6cmGv9fnHWOB6tcfF~hO5ew9et7hBjZzDJIz-luEIo5qxQnoZxBoVh562FL~Pu~G3bkldlRZu4em-Pi7KcNzXG~DC3bYOJjhrqtMz30CCmJNzW6b09Oo79nxJ-4lBSnMQy9FSMKX6R2PSLx1ulCvpKoW5S~WdtpuemBKObQfMITEjMryEu-Jt3Cn2NaTJ8Jdj8C8~A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Modulation_byPolypodium_leucotomosextract_of_cytokine_patterns_in_experimental_trichomoniasis_model","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"The immunomodulating effects of Anapsos®, an aqueous hydrosoluble extract obtained from the rhizomes of the fern Polypodium leucotomos, on both pathogenicity and cytokine levels in serum (IFN-y/IL-4) were assayed in a Trichomonas vaginalis experimental model (BALB/c mice infected with 10 7 trichomonads and examined at day 15 after infection). 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579187"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/111579187/The_relationship_of_experimental_pathogenicity_in_vivo_with_in_vitro_cytoadherence_and_cytotoxicity_of_6_different_isolates_of_Trichomonas_vaginalis"><img alt="Research paper thumbnail of The relationship of experimental pathogenicity in vivo with in vitro cytoadherence and cytotoxicity of 6 different isolates of Trichomonas vaginalis" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">The relationship of experimental pathogenicity in vivo with in vitro cytoadherence and cytotoxicity of 6 different isolates of Trichomonas vaginalis</div><div class="wp-workCard_item"><span>International Journal for Parasitology</span><span>, Aug 1, 1995</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas v...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas vaginalis were studied following their inoculation into NMRI mice and on to adherent cultures of HeLa cells. Contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. The strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate with experimental pathology in vivo.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579187"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579187"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579187; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579187]").text(description); $(".js-view-count[data-work-id=111579187]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579187; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579187']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=111579187]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579187,"title":"The relationship of experimental pathogenicity in vivo with in vitro cytoadherence and cytotoxicity of 6 different isolates of Trichomonas vaginalis","translated_title":"","metadata":{"abstract":"The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas vaginalis were studied following their inoculation into NMRI mice and on to adherent cultures of HeLa cells. Contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. The strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate with experimental pathology in vivo.","publisher":"Elsevier BV","publication_date":{"day":1,"month":8,"year":1995,"errors":{}},"publication_name":"International Journal for Parasitology"},"translated_abstract":"The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas vaginalis were studied following their inoculation into NMRI mice and on to adherent cultures of HeLa cells. Contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. The strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate with experimental pathology in vivo.","internal_url":"https://www.academia.edu/111579187/The_relationship_of_experimental_pathogenicity_in_vivo_with_in_vitro_cytoadherence_and_cytotoxicity_of_6_different_isolates_of_Trichomonas_vaginalis","translated_internal_url":"","created_at":"2023-12-16T10:32:50.016-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_relationship_of_experimental_pathogenicity_in_vivo_with_in_vitro_cytoadherence_and_cytotoxicity_of_6_different_isolates_of_Trichomonas_vaginalis","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The experimental pathogenic effects in vivo and in vitro of 6 different isolates of Trichomonas vaginalis were studied following their inoculation into NMRI mice and on to adherent cultures of HeLa cells. Contact between the parasite and the adherent monolayer of cells was necessary to induce the monolayer to detach. The strains which were more virulent to mice also showed a greater weighted index of adherence; the weighted index of cytotoxicity in vitro did not, on the other hand, correlate with experimental pathology in vivo.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":164,"name":"Parasitology","url":"https://www.academia.edu/Documents/in/Parasitology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":10055,"name":"Cell Adhesion","url":"https://www.academia.edu/Documents/in/Cell_Adhesion"},{"id":22050,"name":"Cytotoxicity","url":"https://www.academia.edu/Documents/in/Cytotoxicity"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":302037,"name":"In Vivo","url":"https://www.academia.edu/Documents/in/In_Vivo"},{"id":422325,"name":"HeLa cells","url":"https://www.academia.edu/Documents/in/HeLa_cells"},{"id":608958,"name":"HeLa","url":"https://www.academia.edu/Documents/in/HeLa"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":4063964,"name":"Trichomonas","url":"https://www.academia.edu/Documents/in/Trichomonas"}],"urls":[{"id":37206596,"url":"https://doi.org/10.1016/0020-7519(95)00001-i"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579183"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579183/Experimental_models_in_Chagas_disease_a_review_of_the_methodologies_applied_for_screening_compounds_against_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Experimental models in Chagas disease: a review of the methodologies applied for screening compounds against Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/109077003/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579183/Experimental_models_in_Chagas_disease_a_review_of_the_methodologies_applied_for_screening_compounds_against_Trypanosoma_cruzi">Experimental models in Chagas disease: a review of the methodologies applied for screening compounds against Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>Parasitology Research</span><span>, Sep 19, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">One of the main problems of Chagas disease (CD), the parasitic infection caused by Trypanosoma cr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">One of the main problems of Chagas disease (CD), the parasitic infection caused by Trypanosoma cruzi, is the lack of a completely satisfactory treatment, which is currently based on two old nitroheterocyclic drugs (i.e., nifurtimox and benznidazole) that show important limitations for treating patients. In this context, many laboratories look for alternative therapies potentially applicable to the treatment, and therefore, research in CD chemotherapy works in the design of experimental protocols for detecting molecules with activity against T. cruzi. Phenotypic assays are considered the most valuable strategy for screening these antiparasitic compounds. Among them, in vitro experiments are the first step to test potential anti-T. cruzi drugs directly on the different parasite forms (i.e., epimastigotes, trypomastigotes, and amastigotes) and to detect cytotoxicity. Once the putative trypanocidal drug has been identified in vitro, it must be moved to in vivo models of T. cruzi infection, to explore (i) acute toxicity, (ii) efficacy during the acute infection, and (iii) efficacy in the chronic disease. Moreover, in silico approaches for predicting activity have emerged as a supporting tool for drug screening procedures. Accordingly, this work reviews those in vitro, in vivo, and in silico methods that have been routinely applied during the last decades, aiming to discover trypanocidal compounds that contribute to developing more effective CD treatments.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4fa498ad4f3aaac6313832bdf583a4ac" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109077003,"asset_id":111579183,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109077003/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579183"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579183"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579183; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579183]").text(description); $(".js-view-count[data-work-id=111579183]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579183; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579183']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4fa498ad4f3aaac6313832bdf583a4ac" } } $('.js-work-strip[data-work-id=111579183]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579183,"title":"Experimental models in Chagas disease: a review of the methodologies applied for screening compounds against Trypanosoma cruzi","translated_title":"","metadata":{"publisher":"Springer Science+Business Media","grobid_abstract":"One of the main problems of Chagas disease (CD), the parasitic infection caused by Trypanosoma cruzi, is the lack of a completely satisfactory treatment, which is currently based on two old nitroheterocyclic drugs (i.e., nifurtimox and benznidazole) that show important limitations for treating patients. In this context, many laboratories look for alternative therapies potentially applicable to the treatment, and therefore, research in CD chemotherapy works in the design of experimental protocols for detecting molecules with activity against T. cruzi. Phenotypic assays are considered the most valuable strategy for screening these antiparasitic compounds. Among them, in vitro experiments are the first step to test potential anti-T. cruzi drugs directly on the different parasite forms (i.e., epimastigotes, trypomastigotes, and amastigotes) and to detect cytotoxicity. Once the putative trypanocidal drug has been identified in vitro, it must be moved to in vivo models of T. cruzi infection, to explore (i) acute toxicity, (ii) efficacy during the acute infection, and (iii) efficacy in the chronic disease. Moreover, in silico approaches for predicting activity have emerged as a supporting tool for drug screening procedures. 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These compounds were subsequently evaluated as inhibitors of biocrystallization of ferriprotoporphyrin IX (heme) to hemozoin, a Plasmodium detoxification specific process. Most bis(5-nitroindazol-3-ols) were good inhibitors, however, a denitro analogue (38), the intermediate bis(3-alkoxyindazoles) (15-29) as well as bis(indazolin-3-ones) (39-42) were not active, showing the importance of the NO(2) and OH groups in the inhibition process.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579181"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579181"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579181; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579181]").text(description); $(".js-view-count[data-work-id=111579181]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579181; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579181']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=111579181]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579181,"title":"Synthesis and Evaluation of 1,1′‐Hydrocarbylenebis(indazol‐3‐ols) as Potential Antimalarial Drugs","translated_title":"","metadata":{"abstract":"Bis(indazol-3-ol) derivatives (5, 30-38) were prepared by alkylation of 3-alkoxyindazoles with alpha,omega-dibromides, followed by removal of the O-protecting groups. 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Most bis(5-nitroindazol-3-ols) were good inhibitors, however, a denitro analogue (38), the intermediate bis(3-alkoxyindazoles) (15-29) as well as bis(indazolin-3-ones) (39-42) were not active, showing the importance of the NO(2) and OH groups in the inhibition process.","internal_url":"https://www.academia.edu/111579181/Synthesis_and_Evaluation_of_1_1_Hydrocarbylenebis_indazol_3_ols_as_Potential_Antimalarial_Drugs","translated_internal_url":"","created_at":"2023-12-16T10:32:47.390-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46646280,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Synthesis_and_Evaluation_of_1_1_Hydrocarbylenebis_indazol_3_ols_as_Potential_Antimalarial_Drugs","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Bis(indazol-3-ol) derivatives (5, 30-38) were prepared by alkylation of 3-alkoxyindazoles with alpha,omega-dibromides, followed by removal of the O-protecting groups. These compounds were subsequently evaluated as inhibitors of biocrystallization of ferriprotoporphyrin IX (heme) to hemozoin, a Plasmodium detoxification specific process. Most bis(5-nitroindazol-3-ols) were good inhibitors, however, a denitro analogue (38), the intermediate bis(3-alkoxyindazoles) (15-29) as well as bis(indazolin-3-ones) (39-42) were not active, showing the importance of the NO(2) and OH groups in the inhibition process.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":531,"name":"Organic Chemistry","url":"https://www.academia.edu/Documents/in/Organic_Chemistry"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":30696,"name":"Hemozoin","url":"https://www.academia.edu/Documents/in/Hemozoin"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":204375,"name":"Heme","url":"https://www.academia.edu/Documents/in/Heme"},{"id":454707,"name":"Antimalarials","url":"https://www.academia.edu/Documents/in/Antimalarials"},{"id":664186,"name":"Alkylation","url":"https://www.academia.edu/Documents/in/Alkylation"},{"id":1330008,"name":"Hemin","url":"https://www.academia.edu/Documents/in/Hemin"},{"id":1692059,"name":"Minimum inhibitory concentration","url":"https://www.academia.edu/Documents/in/Minimum_inhibitory_concentration"},{"id":1693871,"name":"Plasmodium berghei","url":"https://www.academia.edu/Documents/in/Plasmodium_berghei"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"},{"id":4148248,"name":"Minimum Inhibitory Concentration","url":"https://www.academia.edu/Documents/in/Minimum_Inhibitory_Concentration-1"}],"urls":[{"id":37206592,"url":"https://doi.org/10.1002/cmdc.200800176"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579180"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579180/SHORT_COMMUNICATION_Biological_Variability_in_Clinical_Isolates_of_Trichomonas_vaginalis"><img alt="Research paper thumbnail of SHORT COMMUNICATION - Biological Variability in Clinical Isolates of Trichomonas vaginalis" class="work-thumbnail" src="https://attachments.academia-assets.com/109076996/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579180/SHORT_COMMUNICATION_Biological_Variability_in_Clinical_Isolates_of_Trichomonas_vaginalis">SHORT COMMUNICATION - Biological Variability in Clinical Isolates of Trichomonas vaginalis</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Goverment of Madrid. A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fca7b57eb49119c2bb6ff70c5294d9cc" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076996,"asset_id":111579180,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076996/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579180"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579180"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579180; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579180]").text(description); $(".js-view-count[data-work-id=111579180]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579180; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579180']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fca7b57eb49119c2bb6ff70c5294d9cc" } } $('.js-work-strip[data-work-id=111579180]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579180,"title":"SHORT COMMUNICATION - Biological Variability in Clinical Isolates of Trichomonas vaginalis","translated_title":"","metadata":{"grobid_abstract":"Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Goverment of Madrid. 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A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.","owner":{"id":46646280,"first_name":"Jose","middle_initials":"A","last_name":"Escario","page_name":"JoseEscario","domain_name":"ucm","created_at":"2016-04-08T03:17:28.106-07:00","display_name":"Jose A Escario","url":"https://ucm.academia.edu/JoseEscario"},"attachments":[{"id":109076996,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/109076996/thumbnails/1.jpg","file_name":"4525.pdf","download_url":"https://www.academia.edu/attachments/109076996/download_file","bulk_download_file_name":"SHORT_COMMUNICATION_Biological_Variabili.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/109076996/4525-libre.pdf?1702752316=\u0026response-content-disposition=attachment%3B+filename%3DSHORT_COMMUNICATION_Biological_Variabili.pdf\u0026Expires=1743089737\u0026Signature=ckkqohoP9envUAll8WfBoYV5Wbj0EDISJWJSwmETqmPODr9f6~Vvdu6ucni2BKGCII5UIaXjkVydeFeBJ8-OW~PKKuuKJnzt8ny4ZrQRG6ICAyM6UlpKcsOEOU35H-kjO~tMy7m3-o-AGtau2BstgONMqriXu1bVMYyYu5a1FVINKxBPxieyiaBPXZPbbm4b7oQxi~AVfHokRt3BwDlO4etiMuLzp2WJrj-~klOh8izqFAEzMMA0WmfztZ9SWsZ-b1z1IhH0-v66WeOCv87MzSEfXY5ijtZcMs3qo8FovSxEU20vztgtoKBb25L9qHG2pYrEBjYs~kXPqYaughhf4w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":1760295,"name":"Trichomoniasis","url":"https://www.academia.edu/Documents/in/Trichomoniasis"},{"id":4063964,"name":"Trichomonas","url":"https://www.academia.edu/Documents/in/Trichomonas"}],"urls":[{"id":37206590,"url":"https://tspace.library.utoronto.ca/bitstream/1807/20170/4/oc02171.html"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579178"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579178/ChemInform_Abstract_New_Derivatives_of_5_Nitroimidazole_Synthesis_and_Antiparasitic_Activity"><img alt="Research paper thumbnail of ChemInform Abstract: New Derivatives of 5-Nitroimidazole: Synthesis and Antiparasitic Activity" class="work-thumbnail" src="https://attachments.academia-assets.com/109076994/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579178/ChemInform_Abstract_New_Derivatives_of_5_Nitroimidazole_Synthesis_and_Antiparasitic_Activity">ChemInform Abstract: New Derivatives of 5-Nitroimidazole: Synthesis and Antiparasitic Activity</a></div><div class="wp-workCard_item"><span>ChemInform</span><span>, Jun 26, 1990</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ChemInform Abstract In the search for a model tetrahydropteridine which is stable in air and solu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ChemInform Abstract In the search for a model tetrahydropteridine which is stable in air and soluble in organic solvents, the title pteridine derivative (IX) which shows the expected properties is synthesized. (IX) is prepared via the intermediate (V) which can be obtained via two different routes starting from ethyl 5-nitroorotate (I). The second route involves the unexpected formation of the ester (VII).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d64384ab0bacd8f2ddea9155d2080917" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076994,"asset_id":111579178,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076994/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579178"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579178"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579178; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579178]").text(description); $(".js-view-count[data-work-id=111579178]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579178; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579178']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d64384ab0bacd8f2ddea9155d2080917" } } $('.js-work-strip[data-work-id=111579178]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579178,"title":"ChemInform Abstract: New Derivatives of 5-Nitroimidazole: Synthesis and Antiparasitic Activity","translated_title":"","metadata":{"publisher":"Wiley","grobid_abstract":"ChemInform Abstract In the search for a model tetrahydropteridine which is stable in air and soluble in organic solvents, the title pteridine derivative (IX) which shows the expected properties is synthesized. 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(IX) is prepared via the intermediate (V) which can be obtained via two different routes starting from ethyl 5-nitroorotate (I). 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579175"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/111579175/On_the_experimental_activity_of_new_gentian_violet_crystal_violet_derivatives_on_trypanosoma_cruzi"><img alt="Research paper thumbnail of On the experimental activity of new gentian violet crystal violet derivatives on trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">On the experimental activity of new gentian violet crystal violet derivatives on trypanosoma cruzi</div><div class="wp-workCard_item"><span>Revista de Ciencias Farmaceuticas</span><span>, Jun 23, 1991</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579175"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579175"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579175; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="111579174"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111579174/Activity_profile_of_two_5_nitroindazole_derivatives_over_the_moderately_drug_resistant_Trypanosoma_cruzi_Y_strain_DTU_TcII_in_vitro_and_in_vivo_studies"><img alt="Research paper thumbnail of Activity profile of two 5-nitroindazole derivatives over the moderately drug-resistant Trypanosoma cruzi Y strain (DTU TcII): in vitro and in vivo studies" class="work-thumbnail" src="https://attachments.academia-assets.com/109076992/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111579174/Activity_profile_of_two_5_nitroindazole_derivatives_over_the_moderately_drug_resistant_Trypanosoma_cruzi_Y_strain_DTU_TcII_in_vitro_and_in_vivo_studies">Activity profile of two 5-nitroindazole derivatives over the moderately drug-resistant Trypanosoma cruzi Y strain (DTU TcII): in vitro and in vivo studies</a></div><div class="wp-workCard_item"><span>Parasitology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In previous studies, we have identified several families of 5-nitroindazole derivatives as promis...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In previous studies, we have identified several families of 5-nitroindazole derivatives as promising antichagasic prototypes. Among them, 1-(2-aminoethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one, (hydrochloride) and 1-(2-acetoxyethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one (compounds 16 and 24, respectively) have recently shown outstanding activity in vitro over the drug-sensitive Trypanosoma cruzi CL strain (DTU TcVI). Here, we explored the activity of these derivatives against the moderately drug-resistant Y strain (DTU TcII), in vitro and in vivo. The outcomes confirmed their activity over replicative forms, showing IC50 values of 0.49 (16) and 5.75 μm (24) towards epimastigotes, 0.41 (16) and 1.17 μm (24) against intracellular amastigotes. These results, supported by the lack of toxicity on cardiac cells, led to better selectivities than benznidazole (BZ). Otherwise, they were not as active as BZ in vitro against the non-replicative form of the parasite, i.e. bloods...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7343c51f7ce8674625af723164fb1a09" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":109076992,"asset_id":111579174,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/109076992/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111579174"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111579174"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111579174; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111579174]").text(description); $(".js-view-count[data-work-id=111579174]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111579174; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111579174']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7343c51f7ce8674625af723164fb1a09" } } $('.js-work-strip[data-work-id=111579174]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111579174,"title":"Activity profile of two 5-nitroindazole derivatives over the moderately drug-resistant Trypanosoma cruzi Y strain (DTU TcII): in vitro and in vivo studies","translated_title":"","metadata":{"abstract":"In previous studies, we have identified several families of 5-nitroindazole derivatives as promising antichagasic prototypes. Among them, 1-(2-aminoethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one, (hydrochloride) and 1-(2-acetoxyethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one (compounds 16 and 24, respectively) have recently shown outstanding activity in vitro over the drug-sensitive Trypanosoma cruzi CL strain (DTU TcVI). Here, we explored the activity of these derivatives against the moderately drug-resistant Y strain (DTU TcII), in vitro and in vivo. The outcomes confirmed their activity over replicative forms, showing IC50 values of 0.49 (16) and 5.75 μm (24) towards epimastigotes, 0.41 (16) and 1.17 μm (24) against intracellular amastigotes. These results, supported by the lack of toxicity on cardiac cells, led to better selectivities than benznidazole (BZ). Otherwise, they were not as active as BZ in vitro against the non-replicative form of the parasite, i.e. bloods...","publisher":"Cambridge University Press (CUP)","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Parasitology"},"translated_abstract":"In previous studies, we have identified several families of 5-nitroindazole derivatives as promising antichagasic prototypes. Among them, 1-(2-aminoethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one, (hydrochloride) and 1-(2-acetoxyethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one (compounds 16 and 24, respectively) have recently shown outstanding activity in vitro over the drug-sensitive Trypanosoma cruzi CL strain (DTU TcVI). Here, we explored the activity of these derivatives against the moderately drug-resistant Y strain (DTU TcII), in vitro and in vivo. The outcomes confirmed their activity over replicative forms, showing IC50 values of 0.49 (16) and 5.75 μm (24) towards epimastigotes, 0.41 (16) and 1.17 μm (24) against intracellular amastigotes. These results, supported by the lack of toxicity on cardiac cells, led to better selectivities than benznidazole (BZ). 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Among them, 1-(2-aminoethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one, (hydrochloride) and 1-(2-acetoxyethyl)-2-benzyl-5-nitro-1,2-dihydro-3H-indazol-3-one (compounds 16 and 24, respectively) have recently shown outstanding activity in vitro over the drug-sensitive Trypanosoma cruzi CL strain (DTU TcVI). Here, we explored the activity of these derivatives against the moderately drug-resistant Y strain (DTU TcII), in vitro and in vivo. The outcomes confirmed their activity over replicative forms, showing IC50 values of 0.49 (16) and 5.75 μm (24) towards epimastigotes, 0.41 (16) and 1.17 μm (24) against intracellular amastigotes. These results, supported by the lack of toxicity on cardiac cells, led to better selectivities than benznidazole (BZ). 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