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url(https://somalogic.com/wp-content/uploads/2023/02/dataPoints-hero-scaled.jpg);height: 600px;" title="Abstract image of data points"> <div class="container"> <div class="grid"> <div class="cell-md-9"> <p class="breadcrumbs"><a href="/">Home</a> / Platform / Features / <span class="blue">Reproducibility</span></p> <h1>Accurate, reproducible data ensures you get consistently meaningful assay results</h1> <p>Low coefficients of variation allow detection of changes in the abundance of specific proteins in a complex biological sample.</p> </div> </div> </div> </div><div class="wrap white " id="wrap_0" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-10 cell-offset-md-1 cell-sm-12 cell-offset-sm-0" > <div class="a-center mb-60"><h2>Scaling proteomics: SomaScan® Assay maintains precision <em class="blue">from 5K to 7K to 11K</em> protein measurements</h2> </div> </div><div class="text-block cell-md-7 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="pl-40 pr-40"><p>The proteome reflects our biology and complex traits, changing over time in response to internal and external factors such as disease. With so many proteins in so many configurations working in networks that function in cohesive stability, it is necessary to look across the whole proteome in an unbiased approach to find changes that correlate with health outcomes.</p> <p>This suggests that the value of information gained from proteomic studies is directly proportional to the number of proteins measured. However, increasing the range of proteins measured can directly affect the precision of measurement, challenging the accuracy of the data generated.</p> <p>Expanding the coverage of proteomic profiling with SomaScan aptamer-based technology preserves precision, empowering more reproducible data and more meaningful insights with larger scale assays – a capability unmatched with other technologies.</p> </div> </div><div class="text-block cell-md-5 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="pl-40 pr-40 grey-block"><p style="text-align: left"><strong>Read these publications comparing high-plex protein profiling technologies, and concluding that the SomaScan Assay demonstrates the highest precision overall while maintaining this precision as the assay is scaled for increased content.</strong></p> <ul class="blue-check small"> <li style="text-align: left">Eldjarn, GH et al. <a href="https://www.nature.com/articles/s41586-023-06563-x" target="_blank" rel="noopener">Large-scale plasma proteomics comparisons through genetics and disease associations</a>. Nature 622, 348–358 (2023)</li> <li style="text-align: left">Rooney, MR et al. <a href="https://doi.org/10.1101/2024.07.11.24310161" target="_blank" rel="noopener">Plasma proteomic comparisons change as coverage expands for SomaLogic and Olink</a>. medRxiv preprint 2024</li> </ul> </div> </div></div> </div> </div><div class="wrap white desktop pt-30" id="wrap_1" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center mb-60"><h2>With <em class="blue">coefficients of variation ~5%,</em> you can see even the smallest changes in disease state and therapy effectiveness</h2> </div> </div><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><table style="margin: auto"> <tbody> <tr> <th style="background-color: black" colspan="8">Coefficient of Variation (CV) Values</th> </tr> <tr> <th style="background-color: white" colspan="1"></th> <th style="background-color: #ffbdec;color: black" colspan="3">SomaScan® 11K Assay</th> <th colspan="3">SomaScan® 7K Assay</th> </tr> <tr> <td></td> <td style="background-color: #faf8fd"><strong>Intra-Plate</strong></td> <td style="background-color: #faf8fd"><strong>Inter-Plate</strong></td> <td style="background-color: #faf8fd"><strong>Total</strong></td> <td style="background-color: #e2f4f8"><strong>Intra-Plate</strong></td> <td style="background-color: #e2f4f8"><strong>Inter-Plate</strong></td> <td style="background-color: #e2f4f8"><strong>Total</strong></td> </tr> <tr> <td><strong>Plasma</strong></td> <td style="background-color: #faf8fd">3.3%</td> <td style="background-color: #faf8fd">3.0%</td> <td style="background-color: #faf8fd">4.5%</td> <td style="background-color: #e2f4f8">3.6%</td> <td style="background-color: #e2f4f8">3.8%</td> <td style="background-color: #e2f4f8">5.3%</td> </tr> <tr> <td><strong>Serum</strong></td> <td style="background-color: #faf8fd">2.4%</td> <td style="background-color: #faf8fd">2.4%</td> <td style="background-color: #faf8fd">3.5%</td> <td style="background-color: #e2f4f8">2.7%</td> <td style="background-color: #e2f4f8">2.5%</td> <td style="background-color: #e2f4f8">3.8%</td> </tr> </tbody> </table> </div> </div></div> </div> </div><div class="wrap white mobile" id="wrap_2" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><h2>With <em class="blue">coefficients of variation ~5%,</em> you can see even the smallest changes in disease state and therapy effectiveness</h2> </div> </div><div class="text-block cell-md-5 cell-offset-md-1 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><table style="margin: auto"> <tbody> <tr> <th style="background-color: #ffbdec;color: black" colspan="4">SomaScan® 11K Assay<br /> Coefficient of Variation (CV) Values</th> </tr> <tr> <td></td> <td><strong>Intra-Plate</strong></td> <td><strong>Inter-Plate</strong></td> <td><strong>Total</strong></td> </tr> <tr> <td><strong>Plasma</strong></td> <td>3.3%</td> <td>3.0%</td> <td>4.5%</td> </tr> <tr style="background-color: #faf8fd"> <td><strong>Serum</strong></td> <td>2.4%</td> <td>2.4%</td> <td>3.5%</td> </tr> </tbody> </table> </div> </div><div class="text-block cell-md-5 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><table style="margin: auto"> <tbody> <tr> <th colspan="4">SomaScan® 7K Assay<br /> Coefficient of Variation (CV) Values</th> </tr> <tr> <td></td> <td><strong>Intra-Plate</strong></td> <td><strong>Inter-Plate</strong></td> <td><strong>Total</strong></td> </tr> <tr> <td><strong>Plasma</strong></td> <td>3.6%</td> <td>3.8%</td> <td>5.3%</td> </tr> <tr style="background-color: #bdedf1"> <td><strong>Serum</strong></td> <td>2.7%</td> <td>2.5%</td> <td>3.8%</td> </tr> </tbody> </table> </div> </div></div> </div> </div><div class="wrap white align-center pt-30" id="wrap_3" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-5 cell-offset-md-1 cell-sm-12 cell-offset-sm-0" > <div class="a-center pl-40 pr-40"><p><img loading="lazy" decoding="async" class="aligncenter wp-image-4994" style="border: 1px solid lightgray" src="https://somalogic.com/wp-content/uploads/2023/07/CV_White_Paper_Thumbnail-1-232x300.jpg" alt="" width="240" height="311" srcset="https://somalogic.com/wp-content/uploads/2023/07/CV_White_Paper_Thumbnail-1-232x300.jpg 232w, https://somalogic.com/wp-content/uploads/2023/07/CV_White_Paper_Thumbnail-1-791x1024.jpg 791w, https://somalogic.com/wp-content/uploads/2023/07/CV_White_Paper_Thumbnail-1-768x994.jpg 768w, https://somalogic.com/wp-content/uploads/2023/07/CV_White_Paper_Thumbnail-1-193x250.jpg 193w, https://somalogic.com/wp-content/uploads/2023/07/CV_White_Paper_Thumbnail-1.jpg 800w" sizes="auto, (max-width: 240px) 100vw, 240px" /></p> <h3>Why coefficient of variation is a <em>critical metric</em></h3> <p><a class="btn blue" href="https://somalogic.com/white-paper/ensuring-reproducibility-in-proteomics-why-coefficient-of-variation-is-a-critical-metric/">Download the white paper</a></p> </div> </div><div class="text-block cell-md-5 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="grey-block"><p><img loading="lazy" decoding="async" class="aligncenter wp-image-4279" src="https://somalogic.com/wp-content/uploads/2023/04/different-publications.svg" alt="Icon showing publications" width="80" height="76" /></p> <h3>Curated CV research publications</h3> <ul class="blue-check small"> <li style="text-align: left"><a href="https://pubmed.ncbi.nlm.nih.gov/36705086/" target="_blank" rel="noopener">Analytical and Biological Variability of a Commercial Modified Aptamer Assay in Plasma Samples of Patients with Chronic Kidney Disease</a></li> <li style="text-align: left"><a href="https://pubmed.ncbi.nlm.nih.gov/36229504/" target="_blank" rel="noopener">Assessment of variability in the plasma 7k SomaScan proteomics assay</a></li> <li style="text-align: left"><a href="https://pubmed.ncbi.nlm.nih.gov/31639705/" target="_blank" rel="noopener">Reproducibility and Variability of Protein Analytes Measured Using a Multiplexed Modified Aptamer Assay</a></li> </ul> </div> </div></div> </div> </div><div class="wrap black " id="wrap_4" style="background-image: url(https://somalogic.com/wp-content/uploads/2022/07/C3_1_CTA-Texture-1-scaled.jpeg)" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><h2>Leading the industry with dependably low CVs</h2> <h3>No other proteomic platform consistently provides median CVs as low as the SomaScan Platform.</h3> <p>We created the SomaScan Platform with a median CV of ~5%, which means researchers can use the fewest number of samples to detect the most minute biological changes. Without this level of precision, we believe these changes cannot be reliably tested in individuals, and clinical studies will require many more participants.</p> <p><strong>Currently, researchers using other assays see CVs ranging from:</strong><br /> • 5-20% with proximity ligation assays that use polyclonal antibodies<br /> • 10-20% with mass spectrometry</p> </div> </div></div> </div> </div><div class="wrap white mobile" id="wrap_5" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><h2 class="blue">What is the benefit of CVs ~5%?</h2> <p>Median CVs ~5% yield an unmatched level of precision that allows researchers to reliably perform analysis and clinical studies with fewer participants than is required with other proteomic platforms.</p> <p>Relative to the SomaScan Assay with a ~5% CV, a platform with CVs of about 10% would need approximately <em class="blue">2,300 more samples</em> to detect a 1% difference in means compared to detecting that difference using the SomaScan Assay.</p> </div> </div></div> </div> </div><div class="wrap grey mobile full-width-ceros-row" id="MobileCVCalculatorSection" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class=""><div style="position:relative;width:auto;padding:0 0 63.14%;height:0;top:0;left:0;bottom:0;right:0;margin:0;border:0 none" id="experience-6579ef9ebfaf5" data-aspectRatio="1.5837" data-mobile-aspectRatio="0.5625"><iframe title="CV Calc Straight Final 2024 MOBILE" allowfullscreen src="https://view.ceros.com/somalogic/cv-calc-straight-final-1-1" style="position:absolute;top:0;left:0;bottom:0;right:0;margin:0;padding:0;border:0 none;height:1px;width:1px;min-height:100%;min-width:100%" frameborder="0" class="ceros-experience" scrolling="no"></iframe></div><script type="text/javascript" src="https://view.ceros.com/scroll-proxy.min.js" data-ceros-origin-domains="view.ceros.com"></script></div> </div></div> </div> </div><div class="wrap white mobile" id="wrap_7" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><h3>Midplex affinity-based<br /> platforms show median CVs<br /> close to 9% and 12%</h3> <p>The CDF plots reinforce not only the stability of the SomaScan Assay, but also illustrate how the low CVs distinguish the platform from other proteomic technologies.</p> </div> </div><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><p><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-7614" src="https://somalogic.com/wp-content/uploads/2024/03/midplex_Platforms_CVs_2022.jpg" alt="Midplex Platforms CVs chart" width="768" height="658" srcset="https://somalogic.com/wp-content/uploads/2024/03/midplex_Platforms_CVs_2022.jpg 768w, https://somalogic.com/wp-content/uploads/2024/03/midplex_Platforms_CVs_2022-300x257.jpg 300w, https://somalogic.com/wp-content/uploads/2024/03/midplex_Platforms_CVs_2022-292x250.jpg 292w" sizes="auto, (max-width: 768px) 100vw, 768px" /></p> </div> </div></div> </div> </div><div class="wrap grey desktop align-center" id="DesktopCVCalculatorSection" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center mb-60"><h2 class="blue">What is the benefit of CVs ~5%?</h2> <p>Median CVs ~5% yield an unmatched level of precision that allows researchers to reliably perform analysis and clinical studies with fewer participants than is required with other proteomic platforms.</p> <p>Relative to the SomaScan Assay with a ~5% CV, a platform with CVs of about 10% would need approximately <em class="blue">2,300 more samples</em> to detect a 1% difference in means compared to detecting that difference using the SomaScan Assay.</p> </div> </div><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center mb-60"><div style="position:relative;width:auto;padding:0 0 63.14%;height:0;top:0;left:0;bottom:0;right:0;margin:0;border:0 none" id="experience-6558039da54c5" data-aspectRatio="1.5837" data-mobile-aspectRatio="0.5625"><iframe title="CV Calc Straight Final 2024" allowfullscreen src="https://view.ceros.com/somalogic/cv-calc-straight-final-1" style="position:absolute;top:0;left:0;bottom:0;right:0;margin:0;padding:0;border:0 none;height:1px;width:1px;min-height:100%;min-width:100%" frameborder="0" class="ceros-experience" scrolling="no"></iframe></div><script type="text/javascript" src="https://view.ceros.com/scroll-proxy.min.js" data-ceros-origin-domains="view.ceros.com"></script></div> </div><div class="text-block cell-md-5 cell-offset-md-1 cell-sm-12 cell-offset-sm-0" > <div class="pr-40"><h3>Midplex affinity-based<br /> platforms show median CVs<br /> close to 9% and 12%</h3> <p>The CDF plots reinforce not only the stability of the SomaScan Assay, but also illustrate how the low CVs distinguish the platform from other proteomic technologies.</p> </div> </div><div class="text-block cell-md-5 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><p><img loading="lazy" decoding="async" class="wp-image-7614 size-full aligncenter" src="https://somalogic.com/wp-content/uploads/2024/03/midplex_Platforms_CVs_2022.jpg" alt="Midplex Platforms CVs chart" width="768" height="658" srcset="https://somalogic.com/wp-content/uploads/2024/03/midplex_Platforms_CVs_2022.jpg 768w, https://somalogic.com/wp-content/uploads/2024/03/midplex_Platforms_CVs_2022-300x257.jpg 300w, https://somalogic.com/wp-content/uploads/2024/03/midplex_Platforms_CVs_2022-292x250.jpg 292w" sizes="auto, (max-width: 768px) 100vw, 768px" /></p> </div> </div></div> </div> </div><div class="wrap white align-center" id="wrap_9" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-3 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><p><img decoding="async" src="https://somalogic.com/wp-content/uploads/2024/08/biomarkersAbstract_600px.jpg" /></p> </div> </div><div class="text-block cell-md-9 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="pl-40"><h3>Optimizing biomarker discovery with focus on low coefficient of variation in large-scale proteomics</h3> <p>When it comes to biomarker discovery, higher technical variability – which results from higher CVs – means more samples are required to detect a statistically significant difference. Such assay noise can also limit measurements or discoveries, which can mean a loss in detectability or an obstruction of biological effect sizes.</p> </div> </div></div> </div> </div><div class="wrap black " id="wrap_10" style="background-image: url(https://somalogic.com/wp-content/uploads/2022/07/alt-video.jpg)" title="Zoom in of a magnified protein"> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><h2>SOMAmer® Reagents yield precise, reproducible results</h2> <p>You can choose one of our SomaScan® Panels to focus on a specific disease or research need, such as cytokines, cancer, NASH, inflammation, and more. Or customize your own panel.</p> <p><a class="play-button" href="#modal-video"> </a></p> </div> </div></div> </div> </div><div class="wrap white " id="wrap_11" title=""> <div class="container"> <div class="grid"><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><h2>Learn more</h2> </div> </div><div class="cards-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0"> <div class=" cards " ><div class="card black owl-block" style="background-image: url(https://somalogic.com/wp-content/uploads/2022/07/B2_1_Hero-1-scaled.jpeg);" title="Cave explorer shining a light inside cave"> <div> <h3><span>White paper</span>Ensuring reproducibility in proteomics: Why coefficient of variation is a critical metric</h3> <p class="a-center"><a href="https://somalogic.com/white-paper/ensuring-reproducibility-in-proteomics-why-coefficient-of-variation-is-a-critical-metric/" class="btn blue" target="">Download white paper</a></p> </div> </div><div class="card black owl-block" style="background-image: url(https://somalogic.com/wp-content/uploads/2022/07/hero-bg-scaled.jpg);" title="Image of cave explorer"> <div> <h3><span>Poster</span>Optimizing biomarker discovery with focus on low coefficient of variation in large-scale proteomics</h3> <p class="a-center"><a href="https://somalogic.com/poster/optimizing-biomarker-discovery-with-focus-on-low-coefficient-of-variation-in-large-scale-proteomics/" class="btn blue" target="">Download poster</a></p> </div> </div><div class="card black owl-block" style="background-image: url(https://somalogic.com/wp-content/uploads/2022/07/C1_1_Hero-1-scaled.jpeg);" title="Cave explorer shining a light inside a cave"> <div> <h3><span>Webinar</span>Assessment of variability and normalization methods using the plasma SomaScan® 7K Assay v4.1</h3> <p class="a-center"><a href="https://somalogic.com/webinar/assessment-of-variability-and-normalization-methods-using-the-plasma-7k-somascan-assay-v4-1/" class="btn blue" target="">Watch webinar</a></p> </div> </div></div> </div><div class="text-block cell-md-12 cell-offset-md-0 cell-sm-12 cell-offset-sm-0" > <div class="a-center"><h2>Have questions about low <em class="blue">coefficient of variation</em>?</h2> <p><a class="btn blue" href="https://share.hsforms.com/1tuK-RnQUQHy0R2pKjyptxw3zbc6" target="_blank" rel="noopener">Talk with our scientific team.</a></p> </div> </div></div> </div> </div></main> <div class="modal video" id="modal-video"> <div class="modal-wrap"> <div class="modal-content"> <span class="modal-close"></span> <div class="modal-oembed"><script src="https://fast.wistia.com/assets/external/E-v1.js" async></script><div class="wistia_responsive_padding" style="padding:56.25% 0 0 0;position:relative;"><div class="wistia_responsive_wrapper" style="height:100%;left:0;position:absolute;top:0;width:100%;"><div class="wistia_embed wistia_async_n8oi7hv7qf dnt=1 seo=false videoFoam=true" style="height:100%;position:relative;width:100%"><div class="wistia_swatch" style="height:100%;left:0;opacity:0;overflow:hidden;position:absolute;top:0;transition:opacity 200ms;width:100%;"><img src="https://fast.wistia.com/embed/medias/n8oi7hv7qf/swatch" style="filter:blur(5px);height:100%;object-fit:contain;width:100%;" alt="" aria-hidden="true" onload="this.parentNode.style.opacity=1;" /></div></div></div></div></div> <div class="modal-content-wysiwyg"></div> </div> </div> </div> <footer> <div class="footer-top"> <div class="container"> <div class="grid"> <div class="cell-md-6"> <img src="https://somalogic.com/wp-content/themes/soma/img/SomaLogic-SBI-Dual-Logo-White.svg" alt="Somalogic logo" class="footer-logo" /> <p>SomaLogic Operating Co., Inc.<br /> 2945 Wilderness Pl. 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