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id="post-2863" class="post-2863 page type-page status-publish hentry" itemtype="https://schema.org/CreativeWork" itemscope=""> <div class="entry-content entry-content-servises "> <h1>ProLiFiler™ Cancer Cell Panel Screening</h1> <div class="text-mit-bild-text"> <div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"> <p>The ProLiFiler™ service is suitable for testing the therapeutic efficacy of test compounds on a panel of 160 human cancer cell lines using CellTiter-Glo readout.</p> <p>Our cell line panel screening service can answer numerous research questions:</p> <ul> <li>Which tumor cell lines are sensitive to your drug?</li> <li>Which cancer types are susceptible to treatment?</li> <li>What is the genomic signature of your drug for the prediction of anticancer drug response?</li> <li>Which cancer drugs act similar to your drug for confirmation of the mode of action?</li> </ul> <p>Cell line panel screening provides efficacy data for 160 tumor cell lines from across 15 tumor types. The screening is performed with established cell lines to guarantee robust and reproducible results. Identify the most responsive tumor cell lines for subsequent studies on cellular models or animal studies, all of which can be performed with Reaction Biology.</p> <p>Advantages of Reaction Biology’s ProLiFiler™:</p> <ul> <li>Performed on a regular basis</li> <li>Huge cost-savings</li> <li>Fast turnaround time: get your results in 5 to 6 weeks</li> <li>Semi-automated processing for highly reproducible data</li> <li>Single drug or combination drug testing</li> <li>Option for in-depth bioinformatics analysis</li> </ul> </div> <div class="contact-buttons"> <div class="color-scheme-turquoise block block-block-content block-block-content9594f627-1f8b-4f13-b65e-979c19e399c0"> <a href="https://www.reactionbiology.com/contact/contact-us"> <div class="field field--name-field-icon field--type-image field--label-hidden field__items"> <img decoding="async" src="https://www.reactionbiology.com/wp-content/themes/Divi-child/images/icons-email.svg" alt="E-Mail" title="E-Mail" typeof="foaf:Image"> 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decoding="async" src="https://www.reactionbiology.com/wp-content/themes/Divi-child/images/icons-meeting.svg" alt="Meet us at your next conference" title="Meet us at your next conference" typeof="foaf:Image"> </div> <div class="field field--name-field-text field--type-string field--label-hidden field__items"> Meet us at your next conference </div> </a> </div> <div class="color-scheme-turquoise block block-block-content block-block-contentff27f1d7-2f17-4dd2-ad72-3ef66b6982de"> <a href="/cdn-cgi/l/email-protection#c1feb2b4a3aba4a2b5fc88e1b6a0afb5e1b5aee1b2a9a0b3a4e1b5a9a8b2e193a4a0a2b5a8aeafe183a8aeadaea6b8e1ada8afaae1b6a8b5a9e1b8aeb4e7a0acb1faa3aea5b8fca9b5b5b1b2fbeeeeb6b6b6efb3a4a0a2b5a8aeafa3a8aeadaea6b8efa2aeaceeb2a4b3b7a8a2a4b2eea2a4adadeca3a0b2a4a5eca0b2b2a0b8b2eeb1b3aeada8a7a8ada4b3eca2a4adadecada8afa4ecb1a0afa4adecb2a2b3a4a4afa8afa6"> <div class="field field--name-field-icon field--type-image field--label-hidden field__items"> <img decoding="async" src="https://www.reactionbiology.com/wp-content/themes/Divi-child/images/icons-share.svg" alt="Share" title="Share" typeof="foaf:Image"> </div> <div class="field field--name-field-text field--type-string field--label-hidden field__items"> Share </div> </a> </div> </div> </div> </div> </article> </div> </div> <div id="_anchor" class="paragraph weiss black paragraph--type--paragraph-container paragraph--view-mode--default"><div id="cba-id" class="paragraph text-right images-1 headline-left contact-buttons- paragraph--type--text-with-image paragraph--view-mode--default"><div class="container center"><div class="text-wrapper"><h3>CL160-ProLiFiler™</h3> <div class="text-mit-bild-text"> <div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"> <p>The ProLiFiler™ cell panel screening service has 160 cell lines derived from 15 distinct entities with clinically important oncogenic mutations such as kinases, RAS, P53 available for your next cell panel screening project.</p> <p><img loading="lazy" decoding="async" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/CL160-table.png" alt="Cell Line 160 ProLiFiler" width="183" height="363" /></p> </div> </div> <div class="text-mit-bild-bild field field--name-field-images field--type-image field--label-hidden field__items"> <div class="text-mit-bild-bild-rahmen"> <div class="field__item"> <p><img loading="lazy" decoding="async" class="size-full wp-image-1415 alignleft" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/CL160-graph.png" alt="Distribution of Entities in CL-160 ProLiFiler™" width="776" height="800" /></p> <div class="caption">Distribution of Entities in CL-160 ProLiFiler™</div> </div> </div> </div> </div></div></div><div id="cba-id2" class="paragraph paragraph--type--tab-container paragraph--view-mode--default"><div class="container center"><h2>Cell Panel Screening Details</h2> <div class="tab-container"><ul class="tab-navigation"><li class="tab-active" data-id="#tab-cba-id221">Assay Description</li><li data-id="#tab-cba-id222">Reproducibility</li><li data-id="#tab-cba-id223">Assay Setup</li><li data-id="#tab-cba-id224">Screening schedule</li><li data-id="#tab-cba-id225">Case Study - efficacy in tumor cell lines</li><li data-id="#tab-cba-id226">Case Study - Tumor Origins</li><li data-id="#tab-cba-id227">Bioinformatic Analysis</li></ul><div class="field field--name-field-paragraph field--type-entity-reference-revisions field--label-hidden field__items"><div id="tab-cba-id221" class="tab-content-active paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Assay Description</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-1418" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/191021_Cell-Proliferation-Assay.png" alt="assay principle of the cellular proliferation assay performed with celltiter-glo" width="850" height="345" /></p> <p>Cells were seeded in cell culture-treated 384 multi-well plates in the respective medium (A). After overnight incubation at 37 °C, compounds are dispensed by a Nanodrop Tecan D300e machine (B). Cells are treated for 72 h at 37°C at 5 % CO2 (C). Finally, cell plates are equilibrated to room temperature and the viability of cells is determined with <strong>CellTiter-Glo luminescent reagent</strong> based on the amount of ATP present (D).</p> </div></div></div></div><div id="tab-cba-id222" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Reproducibility</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-1418" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/prolifiler_variation_of_reference.png" alt="reproducibility cell proliferation and viability assay on large panel screen" width="850" height="345" /></p> <p>The semi-automated screening process produces highly reproducible data. In every run, a reference compound serves as the control reference compound. Shown here are the mean data and standard error of results from 4 independent ProLiFilerTM runs.</p> </div></div></div></div><div id="tab-cba-id223" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Assay Setup</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><strong>Setup</strong>: IC<sub>50</sub> determination or single concentration testing with CellTiter-Glo readout. Drug combination testing is also an option, see an example study <a href="https://www.reactionbiology.com/wp-content/uploads/2023/07/Drug_combination.pdf">here</a>.</p> <p><strong>Controls</strong>: DMSO-only treatment serves as high control. Staurosporine treatment (at 1×10<sup>-5</sup>M) serves as the low control. In every project, the IC<sub>50</sub> value determination of a reference compound is included.</p> <p><strong>Report</strong>: A detailed report including assay conditions, methodology, and comprehensive evaluation of data as well as raw data for each analysis will be provided.</p> <p><strong>Testing of subsets</strong>: For testing a subset of cell lines, or single concentration screening of multiple compounds, please see our Cell Proliferation Assay service with the free choice setup <a href="/services/cell-based-assays/cell-proliferation-and-viability-assays">here</a>.</p> <p><strong>Screening facility</strong>: The ProLiFiler<sup>TM</sup> cell line panel screening is performed in Freiburg, Germany.</p> <p><strong>Turnaround time:</strong> 5 to 6 weeks</p> <p><strong>Compound requirements</strong>: In brief: 100 µl of the stock solution with 1000x the highest testing concentration or the equivalent as solid material.</p> <ul class="pdf-listing-1" style="margin-bottom: 15px!important;"> <li class="excel"><a class="custom-asset-link-1" title="FAQ Assay Condition Form" href="https://www.reactionbiology.com/faq/" target="_blank" rel="noopener">Please see our FAQ page to access our cell-based assay condition form (German facility) for further details on compound preparation and shipping.</a></li> </ul> </div></div></div></div><div id="tab-cba-id224" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Screening schedule</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p>Please find the screening schedule <a href="/services/cell-based-assays/prolifiler-cell-line-panel-screening/prolifiler-schedule">here</a>. Results are available after 5 to 6 weeks.</p> </div></div></div></div><div id="tab-cba-id225" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Case Study - efficacy in tumor cell lines</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-1418" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/prolifiler_IC50_values_of_crizotinib.png" alt="met kinase inhibitor on large cell line panel screen" width="850" height="345" /></p> <p><strong>Identification of cell lines sensitive to a MET kinase inhibitor</strong>. The MET inhibitor was tested for anti-proliferative and cytotoxic capacity with the ProLiFiler<sup>TM</sup> cell line panel. Three cell lines were most receptive to treatment including MKN-45, LOVO, and Karpas 299.</p> </div></div></div></div><div id="tab-cba-id226" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Case Study - Tumor Origins</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-1418" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/prolifiler_panel_scr_2.png" alt="tumor entity water fall blot after large cell line panel screen" width="850" height="345" /></p> <p><strong>Identification of the tumor origin most sensitive to a MET kinase inhibitor</strong>. IC<sub>50</sub> values of the inhibitor potency on a panel of tumor cell lines were sorted based on the tumor entity. Colon tumors (red) were most susceptible to treatment.</p> </div></div></div></div><div id="tab-cba-id227" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Bioinformatic Analysis</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p>Our bioinformatics analysis tools are available to identify the mechanism of action of your test compound and to detect suitable biomarkers for the stratification of responders vs. non-responders.</p> <p><a href="/resources/tools/moa-finder">MoA Finder</a></p> <p><a href="/services/biomarker-discovery/genetic-biomarkers/biomarker-analysis">Biomarker Analysis</a></p> <p>To offer these analytic tools, we have partnered with 4HF Biotec GmbH, a bioinformatics firm specializing in cancer data mining to discover new anti-cancer drugs. 4HF Biotec performs the data mining analysis based on their proprietary database and provides hands-on support with a high focus on client communication to answer your key questions.</p> </div></div></div></div></div></div></div></div><div id="cba-id3" class="paragraph paragraph--type--form paragraph--view-mode--default"><div class="container center"><div class="text-wrapper"><p><a style="text-decoration: underline;" href="/contact/contact-us/">Contact us</a> to learn more about ProLiFiler™ screen.</p> </div></div></div><div id="cba-id4" class="paragraph paragraph--type--related-resources-container paragraph--view-mode--default"><div class="container center"><h2>Related Resources</h2><div class="field--name-field-paragraph related-resources-reference-wrapper"><div class="paragraph--type--related-resource node--type-related-resource"><div class="related-resource-wrapper"><div class="field--name-field-image"><div class="field field--name-field-image field--type-image field--label-hidden field__items"><img src="https://www.reactionbiology.com/wp-content/uploads/2023/07/ReactionBiology_ProLiFiler_Poster-1.png" alt="A systems biology approach combining ProLiFiler™ and Cancer Data Miner for an enhanced preclinical characterization of the Wee1 inhibitor adavosertib" title="A systems biology approach combining ProLiFiler™ and Cancer Data Miner for an enhanced preclinical characterization of the Wee1 inhibitor adavosertib" typeof="foaf:Image" class="image-style-large"></div></div><div class="related-resource-text"> <div class="field--name-field-resource-category"> Poster </div> <div class="field--name-field-title"> <span class="field field--name-title field--type-string field--label-hidden"> A systems biology approach combining ProLiFiler™ and Cancer Data Miner for an enhanced preclinical characterization of the Wee1 inhibitor adavosertib </span> </div> <div class="field--name-field-download"> <span class="pdfpreview-image-wrapper"> <a 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