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src="https://www.reactionbiology.com/wp-content/uploads/2023/07/cell.jpg" alt="Cellular Angiogenesis Assay"> </div> <div class="container center"> <div class="field field--name-field-headline-big field--type-string field--label-hidden field__items"> <div class="field__item">Cell-Based Assays</div> </div> </div> </div> </div> </div> </div> </div> </section> <div id="main-content"> <div id="block-proquinase-breadcrumbs" class="color-scheme-turquoise block block-system block-system-breadcrumb-block"> <div class="container"> <nav class="breadcrumb" role="navigation" aria-labelledby="system-breadcrumb"> <ol> <li><a href="https://www.reactionbiology.com">Home</a></li> <li>Services</li><li><a href="https://www.reactionbiology.com/services/cell-based-assays/">Cell-based Assays</a></li><li>3D Cell-based Assays</li> <li>Cellular Angiogenesis Assay</li> </ol> </nav> </div> </div> <div class="view-container view-simple-page-conent"> <div class="container"> <article id="post-3168" class="post-3168 page type-page status-publish hentry" itemtype="https://schema.org/CreativeWork" itemscope=""> <div class="entry-content entry-content-servises "> <h1>In Vitro Angiogenesis Assay for Drug Testing</h1> <div class="text-mit-bild-text"> <div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"> <p>The Angiogenesis Assay allows for the evaluation of pro- or anti-angiogenic effects of drugs on HUVEC sprouting capacity on a cellular basis.</p> <p>Angiogenesis, the formation of new blood vessels, is a physiological process during growth and development. Beyond that, angiogenesis is necessary for tumor growth and is involved in other pathological disorders (e.g. psoriasis, macular degeneration).</p> <p>The In Vitro Angiogenesis Assay is a vessel sprouting assay that mimics the in vivo situation with respect to the first stages of the angiogenic cascade, (i) the sprouting in a 3D environment promotes cell-cell signaling between the endothelial cells, (ii) the surrounding matrix is degraded before the invasion, (iii) the spheroids used in the angiogenesis assay generate sprouts that are close to the physiological arrangement of endothelial cell vessels</p> <ul> <li>Vessel sprouting can be induced by VEGF-A, FGF-1, FGF-2, DFO, or others</li> <li>IC50 determination can be performed for angio-stimulating and angio-inhibiting compounds</li> <li>The in vitro angiogenesis assay is reproducible and robust despite its complexity</li> </ul> </div> <div class="contact-buttons"> <div class="color-scheme-turquoise block block-block-content block-block-content9594f627-1f8b-4f13-b65e-979c19e399c0"> <a href="https://www.reactionbiology.com/contact/contact-us"> <div class="field field--name-field-icon field--type-image field--label-hidden field__items"> <img decoding="async" src="https://www.reactionbiology.com/wp-content/themes/Divi-child/images/icons-email.svg" alt="E-Mail" title="E-Mail" typeof="foaf:Image"> </div> <div class="field field--name-field-text field--type-string field--label-hidden field__items"> Contact an expert </div> </a> </div> <div class="color-scheme-turquoise block block-block-content block-block-contentc2ce2ae7-66e6-4f1b-87fb-ded5e6bcfb90"> <a class="newsletter-icon-box" href="#"> <div class="field field--name-field-icon field--type-image field--label-hidden field__items"> <img decoding="async" src="https://www.reactionbiology.com/wp-content/themes/Divi-child/images/icons-newsletter.svg" alt="Subscribe to Newsletter" title="Subscribe to Newsletter" typeof="foaf:Image"> </div> <div class="field field--name-field-text field--type-string field--label-hidden field__items"> Subscribe to our newsletter </div> </a> </div> <div class="color-scheme-turquoise block block-block-content block-block-content427638f8-6c52-46af-9064-2fe39ae6579d"> <a href="/#conferences"> <div class="field field--name-field-icon field--type-image field--label-hidden field__items"> <img decoding="async" src="https://www.reactionbiology.com/wp-content/themes/Divi-child/images/icons-meeting.svg" alt="Meet us at your next conference" title="Meet us at your next conference" typeof="foaf:Image"> </div> <div class="field field--name-field-text field--type-string field--label-hidden field__items"> Meet us at your next conference </div> </a> </div> <div class="color-scheme-turquoise block block-block-content block-block-contentff27f1d7-2f17-4dd2-ad72-3ef66b6982de"> <a href="/cdn-cgi/l/email-protection#07387472656d6264733a4e277066697327736827746f66756227736f6e742755626664736e686927456e686b68607e276b6e696c27706e736f277e687221666a773c6568637e3a6f737377743d28287070702975626664736e6869656e686b68607e2964686a28746275716e6462742864626b6b2a65667462632a667474667e742864626b6b726b66752a6669606e6860626962746e742a667474667e"> <div class="field field--name-field-icon field--type-image field--label-hidden field__items"> <img decoding="async" src="https://www.reactionbiology.com/wp-content/themes/Divi-child/images/icons-share.svg" alt="Share" title="Share" typeof="foaf:Image"> </div> <div class="field field--name-field-text field--type-string field--label-hidden field__items"> Share </div> </a> </div> </div> </div> </div> </article> </div> </div> <div id="_anchor" class="paragraph weiss black paragraph--type--paragraph-container paragraph--view-mode--default"><div id="cba-id" class="paragraph text-right images-1 headline-left contact-buttons- paragraph--type--text-with-image paragraph--view-mode--default"><div class="container center"><div class="text-wrapper"><h3>Vessel sprouting</h3> <div class="text-mit-bild-text"> <div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"> <p>In the angiogenesis assay endothelial cell spheroids will sprout new capillary-like vessels when stimulated with pro-angiogenic factors like VEGF-A.</p> <p>The number and length of the sprouts correspond to the angiogenic activity of the endothelial cells. Pro-angiogenic compounds induce sprouting; whereas angiogenesis inhibitors prevent new vessel formation.</p> </div> </div> <div class="text-mit-bild-bild field field--name-field-images field--type-image field--label-hidden field__items"> <div class="text-mit-bild-bild-rahmen"> <div class="field__item"> <p style="margin-bottom: 0px;"><img loading="lazy" decoding="async" class="size-full wp-image-1415 aligncenter" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/vessel_sprouting.png" alt="angiogenesis assay for vessel sprouting of human endothelial cells in the cell based angiogenesis assay" width="800" height="410" /></p> <div class="caption">angiogenesis assay for vessel sprouting of human endothelial cells in the cell based angiogenesis assay</div> </div> </div> </div> </div></div></div><div id="cba-id2" class="paragraph paragraph--type--tab-container paragraph--view-mode--default"><div class="container center"><h2>Sprouting Assay Details</h2> <div class="tab-container"><ul class="tab-navigation"><li class="tab-active" data-id="#tab-cba-id221">Assay Procedure</li><li data-id="#tab-cba-id222">Example data</li><li data-id="#tab-cba-id223">Screening Formats</li></ul><div class="field field--name-field-paragraph field--type-entity-reference-revisions field--label-hidden field__items"><div id="tab-cba-id221" class="tab-content-active paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Assay Procedure</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p> </p> <p><img decoding="async" class="aligncenter size-full wp-image-1418" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/191020_Cellular-Angiogenesis-Assay_cropped-01.png" alt="assay procedure of cell based angiogenesis assays with human endothelial spheroids" width="690" /></p> <p> </p> <p>Endothelial cells aggregate to form spheroids in a hanging drop system. The spheroids are pipetted into multi-well plates in a collagen matrix where the test compounds and stimulation factors are added. During a 24-hour period, vessels sprout from the spheroids and are quantified by measuring their length to determine the cumulative sprout length.</p> </div></div></div></div><div id="tab-cba-id222" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Example data</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><img decoding="async" class="aligncenter size-full wp-image-1418" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/EC-sprouting-examples.jpg" alt="angiogenesis assay example for sprouting assay" width="690" /></p> <p>Two angiogenesis inhibitors were tested for inhibition of endothelial cell sprouting activated by two factors, VEGF-A or DFO (a metal chelator that induced hypoxia) and fibroblast scattering. In the upper panel, the inhibitor is active on endothelial cell sprouting but does not affect fibroblast scattering. In contrast, the drug in the lower panel inhibits both endothelial cell sprouting and fibroblast scattering.</p> </div></div></div></div><div id="tab-cba-id223" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Screening Formats</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><strong>Setup:</strong> IC<sub>50</sub> value determination and single concentration testing are possible. Drug combination treatment is an option.</p> <p>We offer a variety of stimulating factors for the induction of vessel sprouting such as VEGF-A, DFO, FGF-1, FGF-2, and more.</p> <p>Testing of pro- and anti-angiogenic drug candidates is possible.</p> <p><strong>Controls:</strong> Stimulus-free media only is the low control of vessel sprouting. VEGF-A only is the high control. Treatment with a reference inhibitor is included to ensure quality control.</p> <p><strong>Report:</strong> A detailed report including assay conditions, methodology, and comprehensive evaluation of data as well as raw data for each analysis will be provided. Photographs are optional.</p> <p><strong>Turnaround time:</strong> Results will be available within about 3 weeks.</p> <p><strong>Screening facility:</strong> The assay is performed in Freiburg, Germany.</p> <p><strong>Compound requirements:</strong> In brief: 100 µl of the stock solution with 100x of the highest assay concentration or the equivalent of solid material.</p> <ul class="pdf-listing-1" style="margin-bottom: 15px!important;"> <li class="excel"><a class="custom-asset-link-1" title="FAQ Assay Condition Form" href="https://www.reactionbiology.com/faq/" target="_blank" rel="noopener">Please see our FAQ page to access our cell-based assay condition form (German facility) for further details on compound preparation and shipping.</a></li> </ul> </div></div></div></div></div></div></div></div><div id="cba-id3" class="paragraph paragraph--type--related-resources-container paragraph--view-mode--default"><div class="container center"><h2>Related Resources</h2><div class="field--name-field-paragraph related-resources-reference-wrapper"><div class="paragraph--type--related-resource node--type-related-resource"><div class="related-resource-wrapper"><div class="field--name-field-image"><div class="field field--name-field-image field--type-image field--label-hidden field__items"><img src="https://www.reactionbiology.com/wp-content/uploads/2023/07/Angiogenesis.png" alt="Cellular Angiogenesis Assay" title="Cellular Angiogenesis Assay" typeof="foaf:Image" class="image-style-large"></div></div><div class="related-resource-text"> <div class="field--name-field-resource-category"> Info sheet </div> <div class="field--name-field-title"> <span class="field field--name-title field--type-string field--label-hidden"> Cellular Angiogenesis Assay </span> </div> <div class="field--name-field-download"> <span class="pdfpreview-image-wrapper"> <a href="https://www.reactionbiology.com/wp-content/uploads/2023/07/Cellular_Angiogenesis.pdf" target="_blank">Download PDF</a> </span> </div> </div></div></div><div class="paragraph--type--related-resource node--type-related-resource"><div class="related-resource-wrapper"><div class="field--name-field-image"><div class="field field--name-field-image field--type-image field--label-hidden field__items"><img src="https://www.reactionbiology.com/wp-content/uploads/2024/08/Bakri2024-e1724552159510.png" alt="Vorolanib, sunitinib, and axitinib: A comparative study of vascular endothelial growth factor receptor inhibitors and their anti-angiogenic effects. 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