CINXE.COM
Adaptive Immunosequencing - Adaptive Biotech
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align--text-mobile-center fill fill--navy vertical-padding--none text-wrap--50" style="min-height: 415px; background-repeat:no-repeat;"> <div class="bg " style="background-image: url(https://www.adaptivebiotech.com/wp-content/uploads/2023/05/banner-hero-waves-2.png); min-height: 415px; background-repeat:no-repeat;"> <div class="container" style="min-height: 415px;"> <div class="acf-innerblocks-container"> <h1 class="wp-block-heading has-white-color has-text-color" id="h-adaptive-immunosequencing">Adaptive<br>Immunosequencing</h1> </div> </div> </div> </div> <div class="wp-block-group alignwide is-layout-constrained wp-block-group-is-layout-constrained"> <div style="height:60px" aria-hidden="true" class="wp-block-spacer"></div> <h2 class="wp-block-heading" id="h-adaptive-immunosequencing-0">Adaptive Immunosequencing</h2> <p>Adaptive Immunosequencing provides a quantitative, end-to-end immunosequencing solution that helps you discover the breadth and depth of the adaptive immune system. From experimental design to publication-ready data, this solution gives you the power to decipher the complexity of the adaptive immune system, providing fundamental insights into the body’s response to disease and therapy at the cellular level.</p> <figure class="wp-block-image aligncenter size-large is-style-desktop"><img fetchpriority="high" decoding="async" width="1024" height="530" src="https://adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-1024x530.png" alt="" class="wp-image-25681" srcset="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-1024x530.png 1024w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-300x155.png 300w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-768x398.png 768w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-1536x795.png 1536w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-2048x1061.png 2048w" sizes="(max-width: 1024px) 100vw, 1024px" /></figure> <figure class="wp-block-image aligncenter size-large is-style-mobile"><img decoding="async" width="967" height="1024" src="https://adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-01-967x1024.png" alt="" class="wp-image-25679" srcset="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-01-967x1024.png 967w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-01-283x300.png 283w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-01-768x813.png 768w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-01.png 1126w" sizes="(max-width: 967px) 100vw, 967px" /></figure> <figure class="wp-block-image aligncenter size-large is-style-mobile"><img decoding="async" width="1024" height="879" src="https://adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-02-1024x879.png" alt="" class="wp-image-25680" srcset="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-02-1024x879.png 1024w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-02-300x258.png 300w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-02-768x660.png 768w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Amplification-Mobile-02.png 1126w" sizes="(max-width: 1024px) 100vw, 1024px" /></figure> <div style="height:60px" aria-hidden="true" class="wp-block-spacer"></div> <h2 class="wp-block-heading has-blue-color has-text-color" id="h-accuracy-and-quantification-by-design">Accuracy and quantification by design</h2> <p>Immunosequencing utilizes a multiplex PCR-based assay enabling sequencing directly from genomic DNA. We provide precise and quantitative abundance data on your cell populations—unlike the relative abundance or variable expression data of RNA-based assays. The assay contains rigorously designed synthetic immune templates as in-line controls plus optimized primers that ensure accurate, quantitative and unbiased results with batch-to-batch consistency.</p> <div class="wp-block-group is-layout-constrained wp-container-core-group-is-layout-1 wp-block-group-is-layout-constrained" style="padding-top:var(--wp--preset--spacing--40);padding-bottom:0"> <div class="wp-block-columns is-layout-flex wp-container-core-columns-is-layout-1 wp-block-columns-is-layout-flex"> <div class="wp-block-column is-layout-flow wp-block-column-is-layout-flow"> <ol class="wp-block-list"> <li>First, a synthetic immune repertoire was designed to represent all VJ-gene combinations.</li> </ol> <figure class="wp-block-image size-full has-custom-border"><img loading="lazy" decoding="async" width="875" height="297" src="https://adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-01.png" alt="" class="wp-image-25675" style="border-right-color:var(--wp--preset--color--white);border-right-width:15px;border-left-color:var(--wp--preset--color--white);border-left-width:45px" srcset="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-01.png 875w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-01-300x102.png 300w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-01-768x261.png 768w" sizes="(max-width: 875px) 100vw, 875px" /></figure> </div> <div class="wp-block-column is-layout-flow wp-block-column-is-layout-flow"> <ol class="wp-block-list" start="2"> <li>Then, measuring against our synthetic repertoire, we adjusted our primer concentrations to significantly reduce amplification bias.</li> </ol> <figure class="wp-block-image size-full has-custom-border"><img loading="lazy" decoding="async" width="875" height="297" src="https://adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-02.png" alt="" class="wp-image-25676" style="border-right-color:var(--wp--preset--color--white);border-right-width:15px;border-left-color:var(--wp--preset--color--white);border-left-width:45px" srcset="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-02.png 875w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-02-300x102.png 300w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-02-768x261.png 768w" sizes="(max-width: 875px) 100vw, 875px" /></figure> </div> </div> <div class="wp-block-columns is-layout-flex wp-container-core-columns-is-layout-2 wp-block-columns-is-layout-flex"> <div class="wp-block-column is-layout-flow wp-block-column-is-layout-flow"> <ol class="wp-block-list" start="3"> <li>In-line controls for every sample enable us to measure amplification, correct residual bias, and provide absolute immune cell counts in your sample.</li> </ol> <figure class="wp-block-image size-full has-custom-border"><img loading="lazy" decoding="async" width="876" height="297" src="https://adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-03.png" alt="" class="wp-image-25677" style="border-right-color:var(--wp--preset--color--white);border-right-width:15px;border-left-color:var(--wp--preset--color--white);border-left-width:45px" srcset="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-03.png 876w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-03-300x102.png 300w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-03-768x260.png 768w" sizes="(max-width: 876px) 100vw, 876px" /></figure> </div> <div class="wp-block-column is-layout-flow wp-block-column-is-layout-flow"> <ol class="wp-block-list" start="4"> <li>Our sophisticated bioinformatics platform corrects errors and collapses sequences to deliver highly accurate, high-volume and standardized data sets.</li> </ol> <figure class="wp-block-image size-full has-custom-border"><img loading="lazy" decoding="async" width="875" height="297" src="https://adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-04.png" alt="" class="wp-image-25678" style="border-right-color:var(--wp--preset--color--white);border-right-width:15px;border-left-color:var(--wp--preset--color--white);border-left-width:45px" srcset="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-04.png 875w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-04-300x102.png 300w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_Accuracy-04-768x261.png 768w" sizes="(max-width: 875px) 100vw, 875px" /></figure> </div> </div> </div> <h2 class="wp-block-heading has-blue-color has-text-color" id="h-true-diversity-is-about-seeing-more-of-the-repertoire">True diversity is about seeing more of the repertoire</h2> <p>Immunosequencing delivers the depth and breadth needed to truly capture the immune repertoire. High-throughput sequencing of the CDR3 variable chain of T- and B-cell receptors provides powerful insights. The CDR3 sequence acts as a unique tag for clonal lineage, enabling tracking of T- and B-cells over time, characterizing the diversity of the immune repertoire and measuring the dynamics of disease and treatment.</p> <h2 class="wp-block-heading has-blue-color has-text-color" id="h-services">Services</h2> <div class="wp-block-group is-style-fancy is-layout-constrained wp-container-core-group-is-layout-2 wp-block-group-is-layout-constrained"><noscript class="ninja-forms-noscript-message"> Notice: JavaScript is required for this content.</noscript> <style> /* FORM STYLES */ #nf-form-12-cont { display:block; } </style><style> /* FIELDS STYLES */ .nf-form-content .nf-field-container #nf-field-480-wrap .nf-field-element li .nf-element label:after { background-color:#dd3333; } .nf-form-content .nf-field-container #nf-field-480-wrap .nf-field-element li .nf-element { background-color:#dd3333; } .nf-form-content .nf-field-container #nf-field-486-wrap .nf-field-element .ninja-forms-field { 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For archival specimens, gDNA remains more stable compared to mRNA.</p> <p>With gDNA as starting material, templates can be quantified, providing an absolute cell count. This makes it possible to accurately assess clonal expansion and tissue density of T cells. Complementary DNA (cDNA) or mRNA measurements are obscured by cell expression, because mRNA molecules cannot be directly correlated to cell numbers and may not provide an accurate measure of clonality.</p> <p>When gDNA is interrogated with an unbiased assay, the output includes:</p> <ul class="wp-block-list"> <li>Template quantitation</li> <li>T-cell fraction</li> <li>Absolute cell counts</li> <li>Accurate repertoire metrics</li> </ul> <h2 class="wp-block-heading has-navy-color has-text-color" id="h-hla-classifier">HLA classifier</h2> <p>Foreign antigens are presented to T cells by protein complexes called “major histocompatibility complexes” (MHC) that are coded by human leukocyte antigen (HLA) genes. HLA type plays an important role in driving T-cell selection and in shaping T-cell repertoires. The importance of a person’s HLA type in transplant research is well understood, as is the association of specific HLA alleles to common diseases. New research is emerging that explores the link between HLA and disease susceptibility, response to immunotherapy, and more. By adding the HLA classifier to samples processed using Immunosequencing, researchers can unlock new understanding of immune repertoire dynamics in the context of HLA type.</p> <p>The Immunosequencing assay can infer a sample’s HLA type based on the T-cell receptor (TCR) profile. The HLA classifier uses machine learning to provide a positive or negative call for 145 HLA genes and alleles. This unique tool is based on T-cell receptor sequencing data generated from Adaptive’s partnership with Microsoft and our Antigen Map project from ~4,000 HLA typed samples.</p> <h2 class="wp-block-heading has-blue-color has-text-color" id="h-hla-classifier-specifications">HLA classifier specifications</h2> <figure data-wp-context="{"uploadedSrc":"https:\/\/www.adaptivebiotech.com\/wp-content\/uploads\/2023\/03\/Web-graphics_Desktop_HLA-Classifier.png","figureClassNames":"wp-block-image aligncenter size-large","figureStyles":null,"imgClassNames":"wp-image-25666","imgStyles":null,"targetWidth":2709,"targetHeight":1113,"scaleAttr":false,"ariaLabel":"Enlarge image","alt":""}" data-wp-interactive="core/image" class="wp-block-image aligncenter size-large wp-lightbox-container"><img loading="lazy" decoding="async" width="1024" height="421" data-wp-init="callbacks.setButtonStyles" data-wp-on-async--click="actions.showLightbox" data-wp-on-async--load="callbacks.setButtonStyles" data-wp-on-async-window--resize="callbacks.setButtonStyles" src="https://adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_HLA-Classifier-1024x421.png" alt="" class="wp-image-25666" srcset="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_HLA-Classifier-1024x421.png 1024w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_HLA-Classifier-300x123.png 300w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_HLA-Classifier-768x316.png 768w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_HLA-Classifier-1536x631.png 1536w, https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Web-graphics_Desktop_HLA-Classifier-2048x841.png 2048w" sizes="(max-width: 1024px) 100vw, 1024px" /><button class="lightbox-trigger" type="button" aria-haspopup="dialog" aria-label="Enlarge image" data-wp-init="callbacks.initTriggerButton" data-wp-on-async--click="actions.showLightbox" data-wp-style--right="context.imageButtonRight" data-wp-style--top="context.imageButtonTop" > <svg xmlns="http://www.w3.org/2000/svg" width="12" height="12" fill="none" viewBox="0 0 12 12"> <path fill="#fff" d="M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z" /> </svg> </button></figure> <p>Adaptive also offers a cytomegalovirus (CMV) classifier and a COVID classifier. Contact us to learn more.</p> <h2 class="wp-block-heading has-blue-color has-text-color" id="h-data-output-from-the-hla-classifier">Data output from the HLA classifier</h2> <p>Each of the 145 models are independent and provide a true/false call for that allele based on the number of the relevant TCRs passing that model’s probability cut-off. The classifier model provides two output files. The Summary of Top Calls file lists all the Class I and Class II allele calls for each sample. The Summary of All Results file is a matrix listing all 145 models and the results for each sample indicated by a true/false call.</p> <div style="height:100px" aria-hidden="true" class="wp-block-spacer"></div> <hr class="wp-block-separator has-alpha-channel-opacity is-style-wide"/> <h2 class="wp-block-heading has-navy-color has-text-color" id="h-resources">Resources</h2> <section id="block-block_a82645bb0ecdbc4610fa35971215980f" class="block block--tiles block--tiles--compact"> <div class="tile-grid"> <div class="tile-container tile-linked "> <a href="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Adaptive-Immunosequencing-TCRB-Sample-Preparation-Guidelines22.pdf" class="tile-link"> <h3 class="tile-title">Human TCRB sample preparation guidelines</h3> <div class="tile-link--title">Download</div> </a> </div><!-- .tile-container --> <div class="tile-container tile-linked "> <a href="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Adaptive-Immunosequencing-Human-B-cell-Sample-Preparation-Guidelines.pdf" class="tile-link"> <h3 class="tile-title">Human B-cell sample preparation guidelines</h3> <div class="tile-link--title">Download</div> </a> </div><!-- .tile-container --> <div class="tile-container tile-linked "> <a href="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/immunoSEQ-Mouse-TCRB-Assay-Sample-Preparation-Guidelines89.pdf" class="tile-link"> <h3 class="tile-title">Mouse TCRB sample preparation guidelines</h3> <div class="tile-link--title">Download</div> </a> </div><!-- .tile-container --> <div class="tile-container tile-linked "> <a href="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Adaptive-Immunosequencing-DNA-Extraction-Target-Starting-Quantities35.pdf" class="tile-link"> <h3 class="tile-title">DNA extraction: target starting quantities</h3> <div class="tile-link--title">Download</div> </a> </div><!-- .tile-container --> <div class="tile-container tile-linked "> <a href="https://www.adaptivebiotech.com/wp-content/uploads/2023/03/Adaptive-Immunosequencing-Sample-Packing-and-Shipping-Instructions77.pdf" class="tile-link"> <h3 class="tile-title">Sample packing and shipping instructions</h3> <div class="tile-link--title">Download</div> </a> </div><!-- .tile-container --> </div><!-- .tile-grid --> </section><!-- .block--tiles --> <div style="height:100px" aria-hidden="true" class="wp-block-spacer"></div> <hr class="wp-block-separator has-alpha-channel-opacity is-style-wide"/> <h2 class="wp-block-heading has-navy-color has-text-color" id="h-contact-us">Contact Us</h2> <div class="wp-block-group is-style-fancy is-layout-constrained wp-container-core-group-is-layout-3 wp-block-group-is-layout-constrained"><noscript class="ninja-forms-noscript-message"> Notice: JavaScript is required for this content.</noscript> <style> /* FIELDS STYLES */ .nf-form-content .nf-field-container 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'aria-disabled="true" disabled="true"' : '' }}}> </script><script id='tmpl-nf-field-button' type='text/template'> <button id="nf-field-{{{ data.id }}}" name="nf-field-{{{ data.id }}}" class="{{{ data.classes }}} nf-element"> {{{ ( data.maybeFilterHTML() === 'true' ) ? _.escape( data.label ) : data.label }}} </button> </script><script id="tmpl-nf-field-firstname" type="text/template"> <input type="text" value="{{{ _.escape( data.value ) }}}" class="{{{ data.renderClasses() }}} nf-element" id="nf-field-{{{ data.id }}}" name="{{ data.custom_name_attribute || 'nf-field-' + data.id + '-' + data.type }}" {{{ data.maybeDisableAutocomplete() }}} {{{ data.renderPlaceholder() }}} aria-invalid="false" aria-describedby="nf-error-{{{ data.id }}} nf-description-{{{ data.id }}}" aria-labelledby="nf-label-field-{{{ data.id }}}" {{{ data.maybeRequired() }}} > </script> <script id="tmpl-nf-field-lastname" type="text/template"> <input type="text" value="{{{ _.escape( data.value ) }}}" class="{{{ data.renderClasses() }}} nf-element" id="nf-field-{{{ data.id }}}" name="{{ data.custom_name_attribute || 'nf-field-' + data.id + '-' + data.type }}" {{{ data.maybeDisableAutocomplete() }}} {{{ data.renderPlaceholder() }}} aria-invalid="false" aria-describedby="nf-error-{{{ data.id }}} nf-description-{{{ data.id }}}" aria-labelledby="nf-label-field-{{{ data.id }}}" {{{ data.maybeRequired() }}} > </script> <script id="tmpl-nf-field-recaptcha-v3" type="text/template"> <input type="hidden" name="g-recaptcha-response" /> </script> <script type="text/javascript">window.NREUM||(NREUM={});NREUM.info={"beacon":"bam.nr-data.net","licenseKey":"NRJS-06e8c0ddbe21bad7c96","applicationID":"189529644","transactionName":"M1AGYUIACEtZAUJZCwoaJVZECAlWFxJXVwE=","queueTime":0,"applicationTime":559,"atts":"HxcFFwoaG0U=","errorBeacon":"bam.nr-data.net","agent":""}</script></body> </html>