Design custom NGS panels with Ion AmpliSeq Designer

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <meta name="description" content="Use Ion AmpliSeq Designer, our free and easy yet powerful custom NGS panel design tool, to take your NGS testing in the direction you need." /> <meta name="keywords" content="On-Demand, Spike-in, Made-to-Order, assay design, pcr, Ion Torrent, iontorrents, sequencing, next gen, next gen sequencing, amplicon sequencing, Ion Torrent Systems, semiconductor sequencing, semiconductor sequencing chips, PGM, Personal Genome Machine, Ion, Thermo Fisher Scientific" /> <title>Design custom NGS panels with Ion AmpliSeq Designer</title> <link type="image/x-icon" rel="shortcut icon" href="/resources/images/favicon.ico" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/css/angular/styles.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/css/third-header-footer.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/css/global.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/css/content.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/js/lib/jquery/css/smoothness/jquery-ui-1.8.16.custom.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/css/jquery-ui/jquery-ui-custom.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/js/lib/jquery/css/jquery.alerts-1.1/jquery.alerts.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/js/lib/jquery/css/jquery.msg.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/css/responsive.css?build='v7.8.5-r06a2f73b8b'" /> <link type="text/css" rel="stylesheet" href="/resources/css/font-awesome/all.min.css"/>   <script type="text/javascript"> var require = { "paths": { "datatables": "init/datatables", "datatables-raw": "lib/jquery/jquery.dataTables.min", "hbs": "hbs/hbs", "jquery": "empty:", "jquery.address": "lib/jquery/jquery.address-1.6-5am-modified", "jquery.alerts": "lib/jquery/jquery.alerts-1.1", "jquery.center": "lib/jquery/jquery.center.min", "jquery.cookie": "lib/jquery/jquery.cookie", "jquery.fileupload": "lib/jquery/jquery.fileupload", "jquery.fileupload-validate": "lib/jquery/jquery.fileupload-validate", "jquery.fileupload-process": "lib/jquery/jquery.fileupload-process", "jquery.geneGrid": "lib/geneGrid/jquery.geneGrid", "jquery.geneTable": "lib/geneTable/jquery.geneTable", "jquery.highlight": "lib/jquery/jquery.highlight", "jquery.iframe-transport": "lib/jquery/jquery.iframe-transport", "jquery.jqprint": "lib/jquery/jquery.jqprint-0.3", "jquery.maxlength": "lib/jquery/jquery.maxlength-1.0.3.pack", "jquery.msg": "lib/jquery/jquery.msg.min", "jquery.miller": "lib/miller/jquery.miller", "jquery.measure": "lib/measure/jquery.measure", "jquery.qtip": "lib/jquery/jquery.qtip.min", "jquery.select": "lib/jquery/select2", "jquery.sorted": "lib/sorted/jquery.sorted", "jquery-ui": "lib/jquery/jquery-ui-1.9.2.custom.min", "jquery.ajaxQueue": "lib/jquery/jquery.ajaxQueue.min", "json3": "lib/json3/json3.min", "lodash": "lib/lodash/lodash.min", "d3js": "lib/d3js/d3.min", "scroll": "lib/jquery/scroll", "showdown": "lib/showdown/showdown-0.2.0.min", "uuid": "lib/uuid/uuid", "igv" : "lib/igv/igv-1.0.5", "element": "angular/element" } }; window.require = { baseUrl: "/resources/js", paths: window.require["paths"], urlArgs: "build='v7.8.5-r06a2f73b8b'" }; </script> <script type="text/javascript"> function setDynamicAmpSettings(AMP) { /* * Some information about the current page. */ AMP.currentPageInfo = AMP.namespace('AMP.currentPageInfo'); AMP.currentPageInfo.isLoggedIn = false; /* * Setup some application settings. Start by making sure the settings object is there then add away... */ AMP.settings = AMP.namespace('AMP.settings'); AMP.settings.sessionTimeout = parseInt('30', 1000); AMP.settings.noTargetSelectedMessage='No targets have been selected'; AMP.settings.noEditableTargetsSelectedMessage='Gene type for pre-designed amplicon targets cannot be modified'; AMP.settings.devMode = '' === 'LOCAL' || '' === 'DEV'; AMP.settings.externallyReferencedStatuses = [ 'ORDERED', 'QUOTE_REQUESTED', 'ADDED_TO_CART', 'DIRECT_ORDERED' ]; AMP.settings["minAcceptableTargetsSize"] = 0; AMP.settings["maxMagniSeqAcceptableTargetsSize"] = 1000000; AMP.settings["maxAcceptableTargetsSize"] = 5000000; AMP.settings["designHoldThresholdLow"] = 250000; AMP.settings["designHoldThresholdHigh"] = 1000000; AMP.settings["rnaMinAcceptableTargetsCount"] = 12; AMP.settings["rnaMaxAcceptableTargetsCount"] = 1200; AMP.settings["rnaFusionMinAcceptableTargetsCount"] = -1; AMP.settings["rnaFusionMinAcceptableFusionTargetsCount"] = 1; AMP.settings["rnaFusionMinAcceptableGeneTargetsCount"] = 12; AMP.settings["rnaFusionHDMinAcceptableGeneTargetsCount"] = 19; AMP.settings["sso.enabled"] = "true"; AMP.settings["sso.storeCookies.baseUrl"] = "https:\/\/ampliseq-login.thermofisher.com\/authenticationStatus\/storeCookies"; AMP.settings["regionNameIllegalCharsRegex"] = "[ ,;=\"\'\\\\|\/]"; AMP.settings["externalLinkWarning.validDestinationsRegExp"] = "^https?:\/\/((www|www.(qa5|qa|stage|dev|qa3|qa4))|(qa5|qa|stage|dev|qa3|qa4)).(lifetechnologies|thermofisher).(com|cn)(\/|$)"; AMP.settings["ivgn.auth.soapLogin.fallback.enabled"] = "false"; AMP.settings["cmgt.signout.mixedContent.expected"] = "false"; AMP.settings["support.liveChat.baseUrl"] = "https:\/\/chat.thermofisher.com"; AMP.settings["loginPage.technicalIssue.message"] = "Sorry, but a technical problem occurred during the login process, preventing us from validating your user account against ThermoFisher.com. Please close all browser windows and then try again."; AMP.settings["loginPage.safari.technicalIssue.message"] = "Sorry, but a technical problem occurred during the login process, preventing us from validating your user account against ThermoFisher.com. Since you are using Safari, please take a look at <a href=\"{0}\">Safari Login Help<\/a> for troubleshooting tips."; AMP.settings["loginPage.cookiesDisabled.message"] = "Oops! Your browser seems to have cookies disabled. Make sure cookies are enabled or try opening a new browser window."; AMP.settings["pagination.pageLength.options"] = "15,30,60,100"; AMP.settings["ampliseq.sessionTimeout"] = 30; AMP.settings["oligos.order.minAmplicons.phoenix"] = "This design contains {0} amplicons which does not meet the minimum requirement of at least 12 amplicons in each pool. Please add additional genes to meet the design requirement."; AMP.settings["oligos.order.maxAmplicons.phoenix"] = "This design contains {0} amplicons which exceeds the maximum limit of {1} amplicons. Please remove {2} amplicons to meet the design requirement."; AMP.settings["oligos.order.numberOfPools.phoenix"] = "This design does not meet the requirement that each design have exactly 2-pools. Please add\/remove few more targets to have appropriate number of pools."; AMP.settings["oligos.order.minAmpliconsPerPool.phoenix"] = -1; AMP.settings["oligos.order.maxSpikeInGenes.phoenix"] = "This spike-in design contains {0} genes not available On-Demand, which exceeds the maximum limit of {1} genes. Please remove {2} genes to meet the design requirement."; AMP.settings["oligos.order.minGenes.phoenix"] = "This design contains 0 genes which does not meet the minimum of at least 1 gene. Please add a gene to meet the design requirement."; AMP.settings["oligos.order.maxGenes.phoenix"] = "This design contains {0} genes which exceeds the maximum limit of {1} genes. Please remove {2} genes to meet the design requirement."; AMP.settings["oligos.order.maxAmpliconsAndGenes.phoenix"] = "This design exceeds the maximum limit of {0} amplicons and {1} genes. Please remove at least {2} amplicons and {3} genes to meet the design requirement."; AMP.settings["spikeIn.estimator.maxAmplicons.warning"] = "Estimated Number of Amplicons for Spike-In design is greater than 246 which may lead to an incompatible Spike-In. Please remove a few genes from Spike-In design to have Estimated Amplicons less than 246."; AMP.settings["spikeIn.estimator.maxAmplicons.warning.limit"] = 246; AMP.settings["spikeIn.estimator.unavailable.ampliconCount.warning"] = "The Spike-in amplicon estimates provided may not be accurate since amplicon estimates are currently unavailable for the following genes: "; AMP.settings["invalid.gene.file.prefix"] = "Invalid-gene-"; AMP.settings["invalid.gene.download.header"] = "data:text\/csv;charset=utf-8,"; AMP.settings["target.utr.gene"] = "UTR-only genes are not currently supported in this version of the software. Please visit our <a id=\"asodUtrFAQLink\" href=\"javascript:void(0);\">Ion AmpliSeq On-Demand \u2013 General Panel Design <\/a>section of FAQ for further information."; AMP.settings["asod.challenging.genes.warning.msg.prefix"] = "Notification regarding "; AMP.settings["asod.challenging.genes.warning.msg.suffix"] = "in your design. Please visit <a id=\"challengingGenesFaq\" href=\"javascript:void(0);\">Ion AmpliSeq On-Demand \u2013 Known Challenging Genes <\/a>section of FAQ for further information."; AMP.settings["asod.challenging.gene.list"] = "PKD1,CFTR,DMD,PMS1,PMS2,MYH6,MYH7"; AMP.settings["target.invalid.gene"] = "Invalid gene name\/symbol."; AMP.settings["invalid.gene.file.suffix"] = ".csv"; AMP.settings["assayDesign.maximum.gene.limit.enabled"] = "true"; AMP.settings["assayDesign.maximum.gene.limit"] = 800; AMP.settings["assayDesign.max.gene.limit.exceeded.disease.categories"] = "A design may have a maximum of 800 genes. Please select fewer (or more specific) disease categories."; AMP.settings["design.maxAmpliconLength.warning"] = "Genes with > 325 bp amplicons : "; AMP.settings["design.maxAmpliconLength.suffix"] = ". Refer to the <a id=\"asodMaxAmpliconLength\" href=\"javascript:void(0);\">Ampliseq On-Demand General Panel Design FAQ<\/a> for more information."; AMP.settings["refgene.updated.error.message"] = "Invalid gene symbol. Please refer to <a class=\"refGeneVersionUpdatedFaq\" href=\"javascript:void(0);\">FAQ<\/a> for more details"; AMP.settings["precart.sarscov.genexus.disclaimer"] = "Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0019278_Ion_AmpliSeq_SARS-CoV-2_Research_Panel_Genexus_QR.pdf\"><strong>MAN0019278<\/strong> <\/a> and <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017910_GenexusIntegratedSequencer_UG.pdf\"><strong>MAN0017910<\/strong> <\/a> for library preparation procedure.<br>Number of reactions per panel order: 240 <br><strong>NOTE<\/strong>: Standard tubes will be provided. Additional aliquot step needed to transfer panel to Genexus\u2122 Primer Pool Tubes (Cat. # A40262) before loading onto Genexus instrument for library preparation."; AMP.settings["precart.sarscov.genestudio.manual.disclaimer"] = "Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0019277_Ion_AmpliSeq_SARS-CoV-2_Research_Panel_GeneStudio_QR.pdf\"><strong>MAN0019277<\/strong> <\/a> and <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017003_IonAmpliSeqLibaryKitPlus_UG.pdf\"><strong>MAN0017003<\/strong> <\/a> for library preparation procedure.<br>Number of reactions per panel order: 2250"; AMP.settings["precart.sarscov.genestudio.chef.disclaimer"] = "Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0019277_Ion_AmpliSeq_SARS-CoV-2_Research_Panel_GeneStudio_QR.pdf\"><strong>MAN0019277<\/strong> <\/a> and <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0013432_Ion_AmpliSeq_Library_Prep_on_Ion_Chef_UG.pdf\"><strong>MAN0013432<\/strong> <\/a> for library preparation.<br>Number of reactions per panel order: 600<br><strong>NOTE<\/strong>: 5X primer pools require manual dilution to 2X before library preparation with the Ion AmpliSeq\u2122 Kit for Chef DL8."; AMP.settings["precart.asod.genexus.disclaimer"] = "<span class=\"asterisk-red\">*<\/span>Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017910_GenexusIntegratedSequencer_UG.pdf\"><strong>MAN0017910<\/strong> <\/a> and <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0028005-AmpliSeqCustomAssayGX-UG.pdf\"><strong>MAN0028005<\/strong> <\/a> for library preparation procedure and panel volume usage per reaction.<br><strong>NOTE<\/strong>: Chef-ready tubes will be provided. Additional aliquot step needed to transfer panel to Genexus\u2122 Primer Pool Tubes (Cat. # A40262) before loading onto Genexus instrument for library preparation.<br><span><span class=\"asterisk-red\">^<\/span>Genexus\u2122 Barcodes 32 AS sets support up to 16 reactions on GX5 chip. Genexus\u2122 Barcodes 48 AS sets (only on Genexus 6.8 software version and above) support up to 24 reactions on GX5 & GX7 chips.<\/span>"; AMP.settings["precart.asod.genestudio.manual.disclaimer"] = "<span class=\"asterisk-red\">*<\/span>Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017003_IonAmpliSeqLibaryKitPlus_UG.pdf\"><strong>MAN0017003<\/strong> <\/a> for library preparation procedure and panel volume usage per reaction"; AMP.settings["precart.asod.genestudio.chef.disclaimer"] = "<span class=\"asterisk-red\">*<\/span>Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0013432_Ion_AmpliSeq_Library_Prep_on_Ion_Chef_UG.pdf\"><strong>MAN0013432<\/strong> <\/a> for library preparation and panel volume usage per reaction"; AMP.settings["precart.oncomine.genestudio.manual.disclaimer"] = "<span class=\"asterisk-red\">*<\/span>Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0018518_OncomineTumorSpecificAssay_UG.pdf\"><strong>MAN0018518<\/strong> <\/a> for library preparation procedure and panel volume usage per reaction"; AMP.settings["precart.oncomine.genestudio.chef.disclaimer"] = "<span class=\"asterisk-red\">*<\/span>Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0018518_OncomineTumorSpecificAssay_UG.pdf\"><strong>MAN0018518<\/strong> <\/a> for library preparation and panel volume usage per reaction"; AMP.settings["precart.spikein.genexus.disclaimer"] = "Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017910_GenexusIntegratedSequencer_UG.pdf\"><strong>MAN0017910<\/strong> <\/a> and <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0028005-AmpliSeqCustomAssayGX-UG.pdf\"><strong>MAN0028005<\/strong> <\/a> for library preparation procedure and panel volume usage per reaction.<br><strong>NOTE<\/strong>: Chef-ready tubes will be provided. Additional aliquot step needed to transfer panel to Genexus\u2122 Primer Pool Tubes (Cat. # A40262) before loading onto Genexus instrument for library preparation."; AMP.settings["precart.spikein.genestudio.manual.disclaimer"] = "Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017003_IonAmpliSeqLibaryKitPlus_UG.pdf\"><strong>MAN0017003<\/strong> <\/a> for library preparation (including spike-in procedure) and panel volume usage per reaction.<br><strong>NOTE<\/strong>: Spike-in panels enable extending the content to On-Demand Panels by direct addition of highly-concentrated primers."; AMP.settings["precart.spikein.genestudio.chef.disclaimer"] = "Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0013432_Ion_AmpliSeq_Library_Prep_on_Ion_Chef_UG.pdf\"><strong>MAN0013432<\/strong> <\/a> for library preparation (including spike-in procedure) and panel volume usage per reaction.<br><strong>NOTE<\/strong>: Spike-in panels enable extending the content to On-Demand Panels by direct addition of highly-concentrated primers."; AMP.settings["precart.p40.disclaimer"] = "<p>*Library preparation kit in bundle with the Oncomine Tumor Specific Panel.<\/p>"; AMP.settings["precart.p40.optional.disclaimer"] = "<p>*Library preparation kit in bundle with the Oncomine Tumor Specific Panel.<br><br><span class=\'asterisk-red\'>***<\/span>Required for RNA library preparation, can be optionally purchased with the panel (Set quantity to 0 if it is not required).<\/p>"; AMP.settings["precart.community.methylation.manual.disclaimer"] = "Refer to user bulletin <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017662_Bisulfite_AmpliSeq_Library_Kit_UB.pdf\"><strong>MAN0017662<\/strong> <\/a> for library preparation procedure and panel volume usage per reaction."; AMP.settings["precart.community.methylation.chef.disclaimer"] = "Refer to user bulletin <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017892_Bisulfite_AmpliSeq_Library_Kit_DL8_UB.pdf\"><strong>MAN0017892<\/strong> <\/a> for library preparation procedure and panel volume usage per reaction."; AMP.settings["precart.spikein.formatSelectSelected"] = "Tubes of primer pools only, 50X - Spike in"; AMP.settings["precart.spikein.providedMaterial"] = "2 x 1.5 mL tubes per pool"; AMP.settings["precart.wg.rna.50xspikein.chef"] = "Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0013432_Ion_AmpliSeq_Library_Prep_on_Ion_Chef_UG.pdf\"><strong>MAN0013432<\/strong> <\/a> for library preparation (including spike-in procedure) and panel volume usage per reaction.<br><strong>NOTE<\/strong>: Spike-in panels enable extending the content of compatible Made-to-Order or On-Demand Panels by direct addition of highly-concentrated primers to existing primary panels."; AMP.settings["precart.wg.rna.50xspikein.manual"] = "Refer to User Guide <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017003_IonAmpliSeqLibaryKitPlus_UG.pdf\"><strong>MAN0017003<\/strong> <\/a> for library preparation (including spike-in procedure) and panel volume usage per reaction.<br><strong>NOTE<\/strong>: Spike-in panels enable extending the content of compatible Made-to-Order or On-Demand Panels by direct addition of highly-concentrated primers to existing primary panels."; AMP.settings["panel.cnv.compatibility.111"] = "This solution is compatible for gene CNV detection for the requested target genes that resulted CNV-compatible (see target list for details)."; AMP.settings["panel.cnv.compatibility.000"] = "For a panel to be CNV compatible it should have minimum 60 amplicons, meet the \"80:20 rule\" and have at least 1 CNV compatible gene. This solution is not compatible for CNV detection as it is not meeting the requirements of having at least 1 CNV compatible gene, minimum of 60 amplicons and \"80:20 rule\"."; AMP.settings["panel.cnv.compatibility.001"] = "For a panel to be CNV compatible it should have minimum 60 amplicons, meet the \"80:20 rule\" and have at least 1 CNV compatible gene. This solution is not compatible for CNV detection as it is not meeting the requirements of having at least 1 CNV compatible gene and minimum of 60 amplicons."; AMP.settings["panel.cnv.compatibility.100"] = "For a panel to be CNV compatible it should have minimum 60 amplicons, meet the \"80:20 rule\" and have at least 1 CNV compatible gene. This solution is not compatible for CNV detection as it is not meeting the requirements of having minimum of 60 amplicons and \"80:20 rule\"."; AMP.settings["panel.cnv.compatibility.010"] = "For a panel to be CNV compatible it should have minimum 60 amplicons, meet the \"80:20 rule\" and have at least 1 CNV compatible gene. This solution is not compatible for CNV detection as it is not meeting the requirements of having at least 1 CNV compatible gene and \"80:20 rule\"."; AMP.settings["panel.cnv.compatibility.110"] = "For a panel to be CNV compatible it should have minimum 60 amplicons, meet the \"80:20 rule\" and have at least 1 CNV compatible gene.This solution is not compatible for CNV detection as it is not meeting the requirement of \"80:20 rule\"."; AMP.settings["panel.cnv.compatibility.011"] = "For a panel to be CNV compatible it should have minimum 60 amplicons, meet the \"80:20 rule\" and have at least 1 CNV compatible gene.This solution is not compatible for CNV detection as it is not meeting the requirement of having at least 1 CNV compatible gene."; AMP.settings["panel.cnv.compatibility.101"] = "For a panel to be CNV compatible it should have minimum 60 amplicons, meet the \"80:20 rule\" and have at least 1 CNV compatible gene.This solution is not compatible for CNV detection as it is not meeting the requirement of having minimum 60 amplicons."; AMP.settings['loginPage.safari.technicalIssue.message'] = AMP.settings['loginPage.safari.technicalIssue.message'].replace('{0}', '/help/safariLoginInfo.action'); /* * Some i18n properties that need to be accessed from the javascript. */ AMP.text = AMP.namespace('AMP.text'); AMP.text['tmplDesign.downloadBedFile.dialogTitle'] = 'Download Panel Files'; AMP.text['design.editAll.dialogTitle'] = 'Edit All'; AMP.text['profile.incomplete.title'] = 'Profile Incomplete'; AMP.text['oligos.order.currently.in.cart.message'] = 'This design was already added to the cart, are you sure you want to add it again?'; AMP.text['target.error.gene.name.invalid'] = 'Symbol not recognized. Please submit as genomic coordinates.'; AMP.text['targetType.RNA_GENE'] = 'Gene'; AMP.text['targetType.RNA_REFSEQ'] = 'RefSeq'; AMP.text['errors.failedLogin.unauthorizedUser'] = 'Your username and password are valid, but this account is not allowed to log in. Contact !ampliseq-designs@thermofisher.com to have your username added to the list of allowed users.'; AMP.text['errors.password.mismatch'] = 'Invalid username and/or password, please try again.'; AMP.text['errors.unsupported.user.type.html'] = 'This tool isn\u2019t accessible yet through your eProcurement or B2B, punchout account. In the meantime, please sign in to your Thermo Fisher account to use it. <a href=\"mailto:ampliseq-designs@thermofisher.com\">Customer service<\/a> will be happy to answer any questions you might have.'; AMP.text['errors.external.authentication.service.html'] = 'We apologize for the inconvenience, but the login server is down. Please try again later.'; AMP.text['errors.failedLogin.invalidExternalProfile.html'] = 'The ThermoFisher.com profile associated with your username is currently in an invalid state. Please contact your <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/technical-resources\/contact-us.html\">local customer support<\/a> to have your account enabled for web ordering.'; AMP.text['tmplDesign.edit.confirm.message'] = 'Are you sure you want to modify this design template?'; AMP.text['tmplDesign.edit.confirm.title'] = 'Confirm Design Template Edit'; AMP.text['tmplDesign.edit.cart.special.instructions.required.message'] = 'Instructions for display in cart is required since instructions for email is over 120 characters.'; AMP.text['tmplDesign.edit.cart.special.instructions.required.title'] = 'Cart Special Ordering Instructions Required'; AMP.text['oligos.order.optionalPlateNotIncluded.label'] = 'Tubes of primer pools only'; AMP.text['oligos.order.optionalPlateIncluded.label'] = 'Tubes plus 384 well plates at an <span style="font-weight: bold;">additional cost</span>'; AMP.text['assayDesign.add.noCustomReferencesDialog.messsage'] = 'You have not uploaded any custom references yet. Would you like to upload a custom reference now?'; AMP.text['assayDesign.add.noCustomReferencesDialog.title'] = 'No Custom References'; AMP.text['target.warning.HIGH_EXPRESSION'] = 'This item is highly expressed and may interfere with the panel. Alternative suggestions for a positive control gene are: HMBS, JUN, LRP1, MRPL13 or TBP.'; AMP.text['target.warning.INCOMPATIBLE_TRANSCRIPT'] = 'This transcript is not designed, but a design for the given gene is available.'; AMP.text['target.warning.NOT_DESIGNABLE'] = 'Not designable'; AMP.text['target.warning.INVALID_VERSION'] = 'This transcript version is not designed, but a design for a different version is available.'; AMP.text['oligos.order.phoenix.requestquote'] = 'Requesting Quote...'; AMP.text['assayDesign.submission.name.prefix'] = 'IAD'; AMP.text['assayDesign.submission.magniseq.name.prefix'] = 'IAH'; AMP.text['assayDesign.name.legalCharacters'] = 'The design name must be edited. Design name must be composed of US-ASCII letters, numbers, and spaces, between 3 and 20 characters in length. Permitted symbols are dot (.), dash (-), underscore (_).'; AMP.text['assayDesign.submission.oncomine.name.prefix'] = 'OAD'; AMP.text['assayDesign.results.file.name.prefix'] = 'IAD'; AMP.text['accountProfile.user.email.format'] = 'Invalid Email format'; AMP.text['design.sharing.multiple.users'] = 'design.sharing.multiple.users'; AMP.text['accountProfile.user.email.duplicate'] = 'Duplicate Emails'; AMP.text['design.sharing.shared.already'] = 'The design is already shared with this user'; AMP.text['design.sharing.non.ampliseq.user'] = 'This user does not exist in AmpliSeq'; AMP.text['design.sharing.current.user'] = 'The design cannot be shared with logged in user'; AMP.text['access.code.invalid.error.message'] = 'The Access code must contain a minimum of 6 characters and should not exceed 50 characters'; AMP.text['special.instruction.5X.8tubes.description'] = '1.5ml aliquots with only tubes at 5X concentration - normal scale'; AMP.text['special.instruction.entire.bulk.60tubes.description'] = '1.5ml aliquots with only tubes at 2X concentration - large scale'; AMP.text['special.instruction.5X.24tubes.description'] = '1.5ml aliquots with only tubes at 5X concentration - large scale'; AMP.text['special.instruction.spikeIn.description'] = '1.5ml aliquots for Spike-In use only at 50X concentration in tubes(Subject to upper limit of 246 oligos/123 amplicons per pool)'; AMP.text['special.instruction.384plates.description'] = '384-well plates only (2,250 to 9,000 rxns)'; AMP.text['special.instruction.5X.384plates.description'] = '1.5ml aliquots with tubes at 5X concentration + 384-well plates (2,250 to 9000 rxns)'; AMP.text['special.instruction.one.tube.100nM.description'] = 'Large tubes only at 2X concentration - normal scale Pooled material into a single tube (15mls or 50 mls tubes), no aliquots'; AMP.text['order.summary.disclaimer.mto.dna'] = '*If using half reactions, double the number.'; AMP.text['order.summary.disclaimer.mto.rna'] = 'order.summary.disclaimer.mto.rna'; AMP.text['order.summary.disclaimer.hd'] = '*Primers are provided at 10X, when combined they make 5X.'; AMP.text['order.summary.disclaimer.genexus'] = '*Tubes are provided as 2ml tubes with 1.5mls fill volume. Transfer to Genexus compatible tubes will be required.'; AMP.text['format.selection.title.header.mto'] = 'Our Ion AmpliSeq™ Made-to-Order Panels deliver standard as tubes. If you require plates as well, please select this option below (this selection incurs an additional cost when ordering).'; AMP.text['format.selection.title.header.mto.tubesOnly'] = 'Our Ion AmpliSeq™ Made-to-Order Panels deliver standard as tubes.'; AMP.text['format.selection.tubes.primer.pools.mto'] = '5 or 20 tubes based on amplicons ordered'; AMP.text['format.selection.tubes.plates.mto'] = 'Tubes and 384-well plates provided at additional cost'; AMP.text['format.selection.title.header.hd'] = 'Our Ion AmpliSeq™ HD Made-to-Order panels are delivered as either tubes or 384-well plates, the price is the same regardless of the format selected.'; AMP.text['format.selection.title.header.hd.tubesOnly'] = 'Our Ion AmpliSeq™ HD Made-to-Order panels are delivered as tubes of primer pool only'; AMP.text['format.selection.tubes.primer.pools.hd'] = '12 tubes (6 for FWD and 6 for REV primers)'; AMP.text['format.selection.tubes.plates.hd'] = 'Number of 384-well plates depends on panel size'; AMP.text['order.summary.disclaimer.genexus.asod'] = '*Chef-ready tubes will be provided, additional aliquot step needed to transfer panel to Genexus instrument for library preparation.'; AMP.text['order.summary.genexus.asod.reaction.size'] = '16'; AMP.text['order.summary.genexus.asod.reaction.size.desc'] = '16 to 24'; AMP.text['pre.cart.mto.dna.design.detail'] = 'Ion AmpliSeq™ Made-to-Order DNA panel for'; AMP.text['pre.cart.mto.rna.design.detail'] = 'Ion AmpliSeq™ Made-to-Order RNA panel for'; AMP.text['pre.cart.hd.dna.design.detail'] = 'Ion AmpliSeq™ HD Made-to-Order DNA panel for'; AMP.text['pre.cart.hd.rna.design.detail'] = 'Ion AmpliSeq™ HD Made-to-Order RNA panel for'; AMP.text['pre.cart.asod.dna.design.detail'] = 'Ion AmpliSeq™ On-Demand DNA panel'; AMP.text['pre.cart.p4o.dna.design.detail'] = 'Oncomine™ DNA panel for'; AMP.text['pre.cart.p4o.rna.design.detail'] = 'Oncomine™ RNA panel for'; AMP.text['pre.cart.tmpl.dna.design.detail'] = 'Ion AmpliSeq™ Made-to-Order DNA panel for'; AMP.text['pre.cart.tmpl.rna.design.detail'] = 'Ion AmpliSeq™ Made-to-Order RNA panel for'; AMP.text['pre.cart.p4o.core.design.detail'] = 'Oncomine™ Tumor Specific panel for'; AMP.text['oncomine.addInStock.disabled.tooltip'] = 'Only the DNA content is available for customization at this time'; AMP.text['assayDesign.name.required'] = 'Design name is required.'; AMP.text['pre.cart.consumables.urtkit.error.message'] = 'Quantity should be in multiples of'; AMP.text['pre.cart.p4O.urtkit.default.quantity.24'] = '1'; AMP.text['pre.cart.p4O.urtkit.default.quantity.96'] = '3'; AMP.text['pre.cart.p4O.urtkit.default.quantity.384'] = '12'; AMP.text['pre.cart.p4O.urtkit.default.quantity.32'] = '1'; AMP.text['pre.cart.p4O.urtkit.default.quantity.128'] = '4'; AMP.text['order.summary.covid.manual.reaction.size'] = '2250'; AMP.text['order.summary.covid.ionchef.reaction.size'] = '600'; AMP.text['copy.amplicons.dna.type.validation.message'] = 'Some amplicons in your design exceed the optimal size for the selected input DNA type and may not perform as expected (e.g. 175bp amplicons for use on cfDNA are not recommended). <a class="copyAmpliconFAQ" href="javascript:void(0);">Visit the FAQ for additional details.</a><br><br><b>To ensure proper use, the input DNA type will be automatically converted to accommodate the longer amplicon.</b>'; AMP.text['oncomine.assessment.kit.sku'] = 'A46863'; AMP.text['order.summary.covid.genexus.reaction.size'] = '240'; AMP.text['format.selection.title.header.amplicons.greater.than.500'] = 'Our Ion AmpliSeq&#8482 HD Made-to-Order panels with amplicons greater than 500 amplicons per pool are available in 384-well plates format only.'; AMP.text['sso.login.tfs.refresh.url'] = 'https://www.thermofisher.com/account-center/refresh-token.html'; AMP.text['tfs.china.refresh.url'] = 'https://www.thermofisher.cn/account-center/refresh-token.html'; AMP.text['tfs.row.login.authorize.endpoint'] = 'https://www.thermofisher.com/api-gateway/identity-authorization/identity/oidc/op/authorize?response_type=code'; AMP.text['tfs.row.clientId'] = 'client_id=q3JhCpwGT46OKf2kAPrJISyA'; AMP.text['tfs.row.redirectURI'] = 'redirect_uri=https://ampliseq.com/api-gateway/identity-authorization/identity/auth/token'; AMP.text['tfs.row.scope'] = 'scope=openid%20profile%20email%20username'; AMP.text['tfs.row.state'] = 'state=%7B%22u%22:%22https%3A%2F%2Fwww.thermofisher.com%2Forder%2Fcatalog%2Fen%2FUS%2Fadirect%2Flt%3Fcmd%3DpartnerMktLogin%26LoginData-ReturnURL%3Dhttps://ampliseq.com/login/loginUsingLtSession.action%22,%22t%22:%22regular%22,%22rp%22:%22rp-ampliseq%22%7D'; AMP.text['tfs.china.login.authorize.endpoint'] = 'https://www.thermofisher.cn/api-gateway/identity-authorization/identity/oidc/op/authorize?response_type=code'; AMP.text['tfs.china.clientId'] = 'client_id=Wes5fG4B6dXoOuWKoyugDb9C'; AMP.text['tfs.china.redirectURI'] = 'redirect_uri=https://ampliseq.com/cn/api-gateway/identity-authorization/identity/auth/token'; AMP.text['tfs.china.scope'] = 'scope=openid%20profile%20email%20username'; AMP.text['tfs.china.state'] = 'state=%7B%22u%22:%22https%3A%2F%2Fwww.thermofisher.cn%2Forder%2Fcatalog%2Fzh%2FCN%2Fadirect%2Flt%3Fcmd%3DpartnerMktLogin%26LoginData-ReturnURL%3Dhttps://ampliseq.com/login/loginUsingLtSession.action%22,%22t%22:%22regular%22,%22rp%22:%22rp-ampliseq%22%7D'; AMP.text['tfs.china.registration.url'] = 'https://www.thermofisher.cn/cdcam/identity/registration/rp?response_type=code'; AMP.text['ampliseq.registration.url'] = 'https://www.thermofisher.com/api-gateway/identity/registration/rp?response_type=code'; AMP.text['design.pipeline.steps'] = 'startup,reporter-initial,prepare-custom,prepare-target,prepare-pipe,pick-amplicons,make-amp-master,merge-amp-master,pool,get-solutions,post-process,wrap-up,reporter'; AMP.text['phoenix.tootip.text'] = 'phoenix.tootip.text'; AMP.text['withplates.default.special.instruction'] = 'None (2X + 384-well plates)'; AMP.text['withoutplates.default.special.instruction'] = 'None (2X - Standard scale)'; AMP.text['wg.provided.material'] = 'WG special format'; AMP.text['wg.mto.rna.choosen.format'] = 'Tubes of primer pools only'; AMP.text['withplates.default.special.instruction.wg.rna'] = 'RNA - Tubes in 5X conc. 384 plates using Echo plates. Individual primers at 614uM in Low TE'; AMP.text['withoutplates.default.special.instruction.wg.rna'] = 'RNA - 5X'; AMP.text['otsp.dsg.workflow'] = 'Oncomine Workflow'; AMP.text['otsp.save.customize'] = 'OTSP: Save Customize Btn'; AMP.text['otsp.custom.design.edit'] = 'OTSP: Edit Custom Dsg Btn Clk'; AMP.text['otsp.custom.design.save'] = 'OTSP: Save Finalize Dsg'; AMP.text['otsp.custom.design.finalize'] = 'OTSP: Selected custom panel type:'; AMP.text['otsp.gene.metadata.prev'] = 'OTSP: Gene Info Previous Btn Clk'; AMP.text['otsp.gene.metadata.next'] = 'OTSP: Gene Info Next Btn Clk'; AMP.text['otsp.input.genes.name'] = 'OTSP: Input Genes by name'; AMP.text['otsp.input.genes.file'] = 'OTSP: Input Genes by File'; AMP.text['otsp.request.quote'] = 'OTSP: Request Quote Btn'; AMP.text['otsp.add.to.cart'] = 'OTSP: Add to Cart Btn'; AMP.text['otsp.exclude.core.genes'] = 'OTSP: Exclude All Core Genes'; AMP.text['otsp.include.core.genes'] = 'OTSP: Include All Core Genes'; AMP.text['otsp.exc.associates.genes'] = 'OTSP: Exclude All Associated Genes'; AMP.text['otsp.inc.associates.genes'] = 'OTSP: Include All Associated Genes'; AMP.text['otsp.exc.genes.genelist'] = 'OTSP: Exclude all genes from gene list'; AMP.text['otsp.inc.genes.genelist'] = 'OTSP: Include all genes from gene list'; AMP.text['otsp.exc.added.genes'] = 'OTSP: Exclude All Added Genes'; AMP.text['otsp.inc.added.genes'] = 'OTSP: Include All Added Genes'; AMP.text['otsp.add.instock.genes'] = 'OTSP: Add In Stock Genes Btn Clk'; AMP.text['otsp.gene.cancel'] = 'OTSP: Gene Info Close Btn Click'; AMP.text['otsp.customize.panel'] = 'OTSP: Customize Btn Clk'; AMP.text['otsp.copy.design'] = 'OTSP: Copy Dsg Btn Clk'; AMP.text['otsp.save.copy.design'] = 'OTSP: Save Copy Dsg Btn '; AMP.text['otsp.edit.design'] = 'OTSP: Edit Dsg Name and Details'; AMP.text['otsp.confirm.delete.design'] = 'OTSP: Confirm Delete Dsg Btn Clk'; AMP.text['otsp.confirm.delete.gene'] = 'OTSP: Delete Gene Confirm Btn'; AMP.text['otsp.confirm.restore.design'] = 'OTSP: Restore Dsg Confirm Btn'; AMP.text['otsp.delete.design.link'] = 'OTSP: Delete Dsg Btn Click'; AMP.text['otsp.export.targets'] = 'OTSP: Export Targets Btn Clk'; AMP.text['otsp.restore.targets'] = 'OTSP: Restore Targets Btn Clk'; AMP.text['otsp.finalize'] = 'OTSP: Finalize Dsg Btn Clk'; AMP.text['otsp.finalize.cancel'] = 'OTSP: Finalize Cancel Btn Clk'; AMP.text['otsp.download.files'] = 'OTSP: Download Custom Panel Files'; AMP.text['otsp.download.cancel'] = 'OTSP: Download Core Panel Files Cancel'; AMP.text['otsp.download.core.panel.files'] = 'OTSP: Download Core Panel Files'; AMP.text['otsp.request.quote'] = 'OTSP: Request Quote Btn'; AMP.text['otsp.order.format.next'] = 'OTSP: Format selection Next'; AMP.text['otsp.order.format.back'] = 'OTSP: Format selection Back'; AMP.text['otsp.order.format.close'] = 'OTSP: Format selection Close'; AMP.text['otsp.order.library.next'] = 'OTSP: Library Preparation Next'; AMP.text['otsp.order.library.back'] = 'OTSP: Library Preparation Back'; AMP.text['otsp.order.library.close'] = 'OTSP: Library Preparation Close'; AMP.text['otsp.order.options.next'] = 'OTSP: Order options Next'; AMP.text['otsp.order.options.close'] = 'OTSP: Order options Close'; AMP.text['otsp.order.options.cancel'] = 'OTSP: Order options Cancel'; AMP.text['otsp.input.invalid.file'] = 'OTSP: Invalid input file or content'; AMP.text['asod.dsg.workflow'] = 'ASOD workflow'; AMP.text['asod.dsg.clear.gene'] = 'ASOD: Reset Gene Target Btn'; AMP.text['asod.dsg.add.new.gene'] = 'ASOD: Add Target Btn'; AMP.text['asod.dsg.file.upload'] = 'ASOD: File Upload Btn'; AMP.text['asod.dsg.export.targets'] = 'ASOD: Export Targets Btn'; AMP.text['asod.dsg.edit.name'] = 'ASOD: Edit Dsg Name'; AMP.text['asod.dsg.save.name'] = 'ASOD: Save Dsg Name'; AMP.text['asod.dsg.finalize'] = 'ASOD: Finalize Dsg Btn'; AMP.text['asod.dsg.finalize.save'] = 'ASOD: Save Finalize Dsg Btn'; AMP.text['asod.dsg.amp.distribute'] = 'ASOD: Amp Distribution Btn'; AMP.text['asod.dsg.hide.msg'] = 'ASOD: Hide Messages Btn'; AMP.text['asod.dsg.show.msg'] = 'ASOD: Show Messages Btn'; AMP.text['asod.dsg.clone'] = 'ASOD: Clone Dsg Btn'; AMP.text['asod.cancel.clone'] = 'ASOD: Cancel Clone Btn'; AMP.text['asod.save.clone'] = 'ASOD: Save Clone Btn'; AMP.text['asod.dsg.amp.distribute.download'] = 'ASOD: Amp Distribution Download Btn'; AMP.text['asod.grid.sort'] = 'ASOD: Grid Sort by:'; AMP.text['asod.grid.group'] = 'ASOD: Group By DRA'; AMP.text['asod.igv.zoom'] = 'ASOD: IGV Zoom'; AMP.text['asod.igv.labels'] = 'ASOD: IGV labels Toggle'; AMP.text['asod.igv.config'] = 'ASOD: IGV Configuration'; AMP.text['asod.request.quote'] = 'ASOD: Request Quote Btn'; AMP.text['asod.order.format.next'] = 'ASOD: Format selection Next'; AMP.text['asod.order.format.back'] = 'ASOD: Format selection Back'; AMP.text['asod.order.format.close'] = 'ASOD: Format selection Close'; AMP.text['asod.order.library.next'] = 'ASOD: Library preparation Next'; AMP.text['asod.order.library.back'] = 'ASOD: Library preparation Back'; AMP.text['asod.order.library.close'] = 'ASOD: Library preparation Close'; AMP.text['asod.order.options.next'] = 'ASOD: Order options Next'; AMP.text['asod.order.options.close'] = 'ASOD: Order options Close'; AMP.text['asod.order.options.cancel'] = 'ASOD: Order options Cancel'; AMP.text['create.asod.dsg'] = 'Create ASOD'; AMP.text['create.asod.dra'] = 'ASOD: Dsg created through DRA'; AMP.text['create.asod.dra.clear'] = 'ASOD: Clear DRA Selection'; AMP.text['create.asod.gene.name'] = 'ASOD: Input Genes by name'; AMP.text['create.asod.gene.file'] = 'ASOD: Input Genes by File'; AMP.text['create.asod.gene.list'] = 'ASOD: Dsg created through Gene List'; AMP.text['create.asod.invalid.file'] = 'ASOD: Invalid input file'; AMP.text['mto.dsg.workflow'] = 'MTO Workflow'; AMP.text['mto.request.quote'] = 'MTO: Request Quote Btn'; AMP.text['mto.order.format.next'] = 'MTO: Format selection Next'; AMP.text['mto.order.format.back'] = 'MTO: Format selection Back'; AMP.text['mto.order.format.close'] = 'MTO: Format selection Close'; AMP.text['mto.order.library.next'] = 'MTO: Library preparation Next'; AMP.text['mto.order.library.back'] = 'MTO: Library preparation Back'; AMP.text['mto.order.library.close'] = 'MTO: Library preparation Close'; AMP.text['mto.order.options.next'] = 'MTO: Order options Next'; AMP.text['mto.order.options.close'] = 'MTO: Order options Close'; AMP.text['mto.order.options.cancel'] = 'MTO: Order options Cancel'; AMP.text['mto.switch.design'] = 'MTO: Switch Dsg'; AMP.text['mto.coverage.accept'] = 'MTO RR: Accept Btn in Increase Coverage'; AMP.text['mto.filter.res'] = 'MTO RR: Filter targets by '; AMP.text['mto.download.res'] = 'MTO RR: Download Assay Results'; AMP.text['mto.download.res.dialog'] = 'MTO RR: Download Assay Results Dialog'; AMP.text['mto.export.proceed'] = 'MTO RR: Proceed Export'; AMP.text['mto.ucsc.link'] = 'MTO RR: UCSC Linkout'; AMP.text['mto.hotspot.toggle'] = 'MTO RR: Accordion (Hotspot)'; AMP.text['hd.dsg.workflow'] = 'hd.dsg.workflow'; AMP.text['hd.request.quote'] = 'HD: Request Quote Btn'; AMP.text['hd.order.format.next'] = 'HD: Format selection Next'; AMP.text['hd.order.format.back'] = 'HD: Format selection Back'; AMP.text['hd.order.format.close'] = 'HD: Format selection Close'; AMP.text['hd.order.library.next'] = 'HD: Library preparation Next'; AMP.text['hd.order.library.back'] = 'HD: Library preparation Back'; AMP.text['hd.order.library.close'] = 'HD: Library preparation Close'; AMP.text['hd.order.options.next'] = 'HD: Order options Next'; AMP.text['hd.order.options.close'] = 'HD: Order options Close'; AMP.text['hd.order.options.cancel'] = 'HD: Order options Cancel'; AMP.text['hd.switch.design'] = 'HD: Switch Dsg'; AMP.text['hd.coverage.accept'] = 'HD DNA RR: Accept Btn in Increase Coverage'; AMP.text['hd.filter.res'] = 'HD RR: Filter targets by '; AMP.text['hd.download.res'] = 'MTO RR: Download Assay Results'; AMP.text['hd.download.res.dialog'] = 'HD RR: Download Assay Results Dialog'; AMP.text['hd.export.proceed'] = 'HD RR: Proceed Export'; AMP.text['hd.ucsc.link'] = 'HD RR: UCSC Linkout'; AMP.text['hd.hotspot.toggle'] = 'Community Panel'; AMP.text['com.dsg.workflow'] = 'HD RR: Accordion (Hotspot)'; AMP.text['wg.dsg.workflow'] = 'WG Workflow'; AMP.text['comm.request.quote'] = 'Community Panel: Request Quote Btn'; AMP.text['wg.request.quote'] = 'WG: Request Quote Btn'; AMP.text['wg.add.to.cart'] = 'WG: Add to Cart Btn'; AMP.text['comm.add.to.cart'] = 'Community Panel: Add to Cart Btn'; AMP.text['mto.add.to.cart'] = 'MTO: Add to Cart Btn'; AMP.text['asod.add.to.cart'] = 'ASOD: Add to Cart Btn'; AMP.text['otsp.add.to.cart'] = 'OTSP: Add to Cart Btn'; AMP.text['order.journey'] = 'Order Journey Workflow'; AMP.text['order.journey.link.plates'] = 'OJ Links: Learn more - With plates'; AMP.text['order.journey.link.noplates'] = 'OJ Links: Learn more - Without plates'; AMP.text['order.journey.link.hd.noplates'] = 'OJ Links: Learn more - HD dsgs Without plates'; AMP.text['order.journey.link.hd.plates'] = 'OJ Links: Learn more - HD designs With plates'; AMP.text['pcp.workflow'] = 'PCP Workflow'; AMP.text['pcp.submit'] = 'PCP: Submit Btn '; AMP.text['pcp.btn'] = 'PCP: Propose as Comm Panel Btn '; AMP.text['survey.workflow'] = 'Survey Toast'; AMP.text['survey.close'] = 'ST: Survey pop-up close'; AMP.text['survey.start'] = 'ST: Started survey for '; AMP.text['survey.maybe'] = 'ST: Maybe later for '; AMP.text['survey.no'] = 'ST: Not for '; AMP.text['user.session'] = 'User Session'; AMP.text['session.logout'] = 'User Session: Auto logout'; AMP.text['session.renew'] = 'User Session: Renew'; AMP.text['pagination'] = 'Pagination'; AMP.text['page.click'] = 'Page Length for pagination'; AMP.text['otsp.order.options.back'] = 'OTSP: Order options Back'; AMP.text['order.pre.workflow'] = 'order.pre.workflow'; AMP.text['otsp.req.quote.print'] = 'OTSP: Print Request QuoteÂ Btn'; AMP.text['asod.req.quote.print'] = 'ASOD: Print Request QuoteÂ Btn'; AMP.text['mto.req.quote.print'] = 'MTO: Print Request QuoteÂ Btn'; AMP.text['hd.req.quote.print'] = 'HD: Print Request QuoteÂ Btn'; AMP.text['comm.req.quote.print'] = 'Community Panel: Print Request QuoteÂ Btn'; AMP.text['wg.req.quote.print'] = 'WG: Print Request QuoteÂ Btn'; AMP.text['otsp.req.quote.done'] = 'OTSP: Request Quote Done Btn'; AMP.text['asod.req.quote.done'] = 'ASOD: Request Quote Done Btn'; AMP.text['mto.req.quote.done'] = 'MTO: Request Quote Done Btn'; AMP.text['hd.req.quote.done'] = 'HD: Request Quote Done Btn'; AMP.text['comm.req.quote.done'] = 'Community Panel: Request Quote Done Btn'; AMP.text['wg.req.quote.done'] = 'WG: Request Quote Done Btn'; AMP.text['otsp.req.quote.close'] = 'OTSP: Request Quote Cancel Btn'; AMP.text['asod.req.quote.close'] = 'ASOD: Request Quote Cancel Btn'; AMP.text['mto.req.quote.close'] = 'MTO: Request Quote Cancel Btn'; AMP.text['hd.req.quote.close'] = 'HD: Request Quote Cancel Btn'; AMP.text['comm.req.quote.close'] = 'Community Panel: Request Quote Cancel Btn'; AMP.text['wg.req.quote.close'] = 'WG: Request Quote Cancel Btn'; AMP.text['otsp.clear.quantity'] = 'OTSP: Clear All Quantities Btn'; AMP.text['asod.clear.quantity'] = 'ASOD: Clear All Quantities Btn'; AMP.text['mto.clear.quantity'] = 'MTO: Clear All Quantities Btn'; AMP.text['hd.clear.quantity'] = 'HD: Clear All Quantities Btn'; AMP.text['comm.clear.quantity'] = 'Community Panel: Clear All Quantities Btn'; AMP.text['wg.clear.quantity'] = 'WG: Clear All Quantities Btn'; AMP.text['otsp.precart.cancel'] = 'OTSP: Cancel Btn'; AMP.text['asod.precart.cancel'] = 'ASOD: Cancel Btn'; AMP.text['mto.precart.cancel'] = 'MTO: Cancel Btn'; AMP.text['hd.precart.cancel'] = 'HD: Cancel Btn'; AMP.text['comm.precart.cancel'] = 'Community Panel: Cancel Btn'; AMP.text['wg.precart.cancel'] = 'WG: Cancel Btn'; AMP.text['otsp.precart.sos'] = 'OTSP: Download SOS Btn'; AMP.text['asod.precart.sos'] = 'ASOD: Download SOS Btn'; AMP.text['mto.precart.sos'] = 'MTO: Download SOS Btn'; AMP.text['hd.precart.sos'] = 'HD: Download SOS Btn'; AMP.text['comm.precart.sos'] = 'Community Panel: Download SOS Btn'; AMP.text['wg.precart.sos'] = 'WG: Download SOS Btn'; AMP.text['wg.comm.mini.pack.choosen.format'] = 'Tubes of primer pools (Small)'; AMP.text['wg.mto.mini.pack.provided.material'] = '1 X 1.5ml tubes per pool'; AMP.text['wg.hd.mini.pack.provided.material'] = '2 X 1.5ml tubes (1 FWD/1 REV) per pool'; AMP.text['comm.mini.pack.provided.material'] = '1 tube per pool'; if (window.pageSpecificTexts) { AMP.text = $.extend({}, AMP.text, window.pageSpecificTexts); } window.pageSpecificTexts = AMP.text; return AMP; } </script> <script type="text/javascript"> function setAmpNavigationDestinations(AMP) { /* * Add some destinations to the navigation namespace. */ AMP.navigation.destinations = { contextPath: '', editUserProfile: '/protected/account/edit-user-profile.action', editUserAccount: '/protected/account/edit-user-account.action', dashboard: '/protected/dashboard.action', dashboardDesignTable: '/protected/dashboard/designsTable.action', designDashboard: '/protected/designDashboard.action', deleteDesign : '/protected/design/delete.action', updateDesign : '/protected/designdata/contents/update.action', geneSymbolAutocomplete: '/protected/genes/provideGeneSuggestions.action', sequenceList: '/protected/sequences/sequences-list.action', chromosomeAutocomplete: '/protected/genes/chromosomeSuggestions.action', snpIdAutocomplete: '/protected/snp/provideSnpIdSuggestions.action', coverageSummaryList: '/protected/designresult/contents/listCoverageSummary.action', coverageDetailsList: '/protected/designresult/contents/listCoverageDetails.action', ampliconDetailsList: '/protected/designresult/contents/listAmpliconDetails.action', tmplCoverageSummaryList: '/tmpl/list/coverageSummary.action', tmplCoverageDetailsList: '/protected/tmpl/list/coverageDetails.action', tmplAmpliconDetailsList: '/protected/tmpl/list/ampliconDetails.action', designSolutionsList: '/protected/designsolution/contents/list.action', updateCurrentDesignSolution: '/protected/designsolution/updateCurrentDesignSolution.action', addDesignSolutionToCart: '/protected/cart/addDesignSolutionToCart.action', addFixedPanelToCart: '/protected/cart/addFixedPanelToCart.action', viewCart: '/protected/cart/viewCart.action', shoppingCarts: 'https://www.thermofisher.com/order/catalog/en/US/direct/lt?cmd=WorkspaceActiveOILListRequest', oligosOrderList: '/protected/oligosorder/oligosOrderHistory.action', login: '/login/login.action', loginUsingLtSession: '/login/loginUsingLtSession.action', sessionExternalCheck: '/protected/sessionExternalCheck.action', sessionExternalRevalidation: '/protected/contents/sessionExternalRevalidation.action', logout: '/logout/logout.action', sessionCheck: '/sessionCheck.action', sessionRenew: '/sessionRenew.action', orderOligosEntry: '/protected/oligosorder/contents/edit.action', orderOligosSave: '/protected/oligosorder/contents/save.action', formatSelectionForm: '/protected/oligosorder/contents/formatSelectionForm.action', refreshTargetsList: '/protected/designdata/contents/refresh.action', rnaTargetRowUpdateUrl: '/protected/rnatarget/contents/save.action', targetListDataTable: '/protected/target/targetsTable.action', rnaSummaryData: '/protected/designresult/rnaSummaryData.action', rnaDetailsData: '/protected/designresult/rnaDetailsData.action', rnaChangeStatus: '/protected/designresult/rnaChangeSummaryStatus.action', rnaWarningIcon: '/resources/images/icon-rna-warning_16x16.png', retrieveSolutionOrderableInfo: '/protected/oligosorder/retrieveSolutionOrderableInfo.action', cmgtSignoutFullPath: 'https://www.thermofisher.com/order/catalog/en/US/adirect/lt?cmd=LogoutDisplay', cmgtSignoutSecondaryFullPath: '', tmplAccessionSummaries: '/tmpl/rna/accessionSummaries.action', tmplAccessionDetails: '/tmpl/rna/accessionDetails.action', errorPage: '/error.jsp', errorPage500: '/500.jsp', usersAutocomplete: '/admin/users/provideUserSuggestions.action', tmplView: '/protected/tmpl/view.action', publicTmplView: '/tmpl/view.action', externalLinkWarning: '/protected/leavingSite.action', acceptHiddenSolutionsDisclaimer: '/protected/designsolution/acceptDisclaimer.action', designCoverageSummaryDataTable: '/protected/designresult/hotspot/coverageSummary/dataTable.action', designAmpliconDetailDataTable: '/protected/designresult/hotspot/ampliconDetail/dataTable.action', sharedDesign: '/protected/shared/viewSharedDesign.action', profileValidationMessages: '/protected/account/contents/profileValidationMessages.action', customReferenceTable: '/protected/references/referencesTable.action', customReferenceEdit: '/protected/references/edit.action', customReferenceView: '/protected/references/view.action', customReferenceSequencesTable: '/protected/references/sequencesDataTable.action', customReferenceVariantsTable: '/protected/references/variantsDataTable.action', customReferenceCreateFromNewDesignPage: '/protected/design/createCreateReferenceFromNewDesignPage.action', temporaryFileUpload: '/protected/temporary-file-upload.action', sequencesFileUpload: '/protected/sequences-file-upload.action', requestMaxCustomReferenceIncrease: '/protected/references/contents/request-max-custom-reference-increase.action', adminDesignsInProgressDetail: '/admin/designs/inprogress/admin-designs-in-progress-detail.action', reUseDesignAmpliconsForm: '/protected/reuseamplicons/contents/reUseAmpliconsForm.action', reUseTmplAmpliconsForm: '/protected/tmpl/reuseamplicons/contents/reUseAmpliconsForm.action', downloadDesignAmplicons: '/protected/reuseamplicons/contents/downloadAmplicons.action', downloadTmplAmplicons: '/protected/tmpl/reuseamplicons/contents/downloadAmplicons.action', designSelectionFormForDesignAmplicons: '/protected/reuseamplicons/contents/designSelectionForm.action', designSelectionFormForTmplAmplicons: '/protected/tmpl/reuseamplicons/contents/designSelectionForm.action', targetsDataTable: '/protected/target/dataTable.action', targetSave: '/protected/target/contents/save.action', targetAmpliconsDataTable: '/protected/target/amplicon/dataTable.action', solutionFinder: '/protected/solutionFinder/redirect.action', designSummaryData: '/protected/design/contents/designSummary.action', dnaConfirmDesignSubmission: '/protected/design/dna/contents/confirmDesignSubmission.action', fusionPairAutocomplete: '/protected/fusion/provideFusionPairSuggestions.action', fusionTranscriptsForPair: '/protected/fusion/findTranscriptsForPair.action', fusionTargetsForDesign: '/protected/target/fusions/list.action', rnaFusionResultsDataTable: '/protected/designresult/rna-fusion-results-table.action', rnaFusionTranscriptsDataTable: '/protected/designresult/rna-fusion-transcripts-table.action', rnaFusionRestorePrePopulatedGenes: '/protected/designdata/contents/restorePrePopulatedGenes.action', calculatorTableData: '/chip/chip-table-data.action', calculatorTmplSolutions: '/chip/tmpl-solutions.action', calculatorCustomSolution: '/chip/custom-solution.action', diseaseReferenceTable: '/protected/inventoried/diseaseReferenceTable.action', diseaseTaxonomy: '/protected/inventoried/diseaseTaxonomy.action', diseaseDescendantCategories: '/protected/inventoried/diseaseDescendantCategories.action', validateGene: '/protected/inventoried/validate-gene.action', orderVirtualPanel: '/protected/inventoried/order-virtual-panel.action', getGeneMap: '/protected/inventoried/get-gene-map.action', getCategorizedGenes: '/protected/inventoried/get-categorized-genes.action', resolveGeneAssociations: '/protected/inventoried/resolve-gene-associations.action', categoriesBySymbol: '/protected/inventoried/diseaseCategoriesForSymbol.action', validateAssayName: '/protected/inventoried/contents/validateAssay.action', searchPanel : '/protected/panels/search.action', editVirtualPanel: '/protected/inventoried/editVirtualPanel.action', changeDisabledStatus : '/protected/inventoried/contents/changeDisabledStatus.action', getTargetMetaInfo : '/protected/inventoried/contents/getTargetMetaInfo.action', updateVirtualPanel : '/protected/inventoried/contents/updateVirtualPanel.action', deleteGeneTargets : '/protected/inventoried/contents/deleteGeneTargets.action', orderingOptions : '/protected/oligosorder/get-ordering-options.action', phoenixOligosOrderSave : '/protected/phoenix/oligosorder/contents/save.action', igvContextGenerator: '/protected/tmpl/contents/view/igv-context-builder.action', updateDesigns: '/protected/dashboard/updateDesigns.action', designBySymbol : '/protected/newDesignBySymbol.action', designByCategory : '/protected/newDesignByCategory.action', designByCustom : '/protected/newDesign.action', designByMagniseq : '/protected/ampliseqhdDesign.action', clonePage : '/protected/inventoried/contents/copyFrom.action', getAlerts : '/protected/loadMessage.action', startPage : '/protected/startPage.action', createCPPanel : '/protected/inventoried/createCPPanel.action', resolvePipelineGeneSynonymAssociation : '/protected/inventoried/resolve-gene-synonym-association.action', publicGenome : '/references/index.action', updateDesignName : '/protected/inventoried/contents/updateDesignName.action', updateDesignGeneVersion : '/protected/inventoried/contents/updateDesignGeneVersion.action', validateSpikeInSubmission : '/protected/design/contents/validateSpikeInSubmission.action', refreshDesignStats : '/protected/inventoried/contents/refreshStats.action', validateDRAGeneSize : '/protected/inventoried/contents/validateMaxGeneLimit.action', updateDesignTypes : '/protected/reuseamplicons/contents/designsBasedOnType.action', ampliconSizes : '/protected/reuseamplicons/contents/ampliconSizes.action', ampliconTmplSizes : '/protected/tmpl/reuseamplicons/contents/ampliconSizes.action', loadDNATypeSelectionForm : '/protected/design/contents/loadDNATypeSelectionForm.action', saveDNAType: '/protected/design/contents/submitAmpliconSizeValue.action', onHoldList : '/admin/designs/onhold/list.action', designsInProgress : '', downloadExcludedAmplicons : '/protected/design/amplicon/download.action', downloadConflictingAmplicons : '/protected/designsolution/amplicon/downloadConflictingAmplicons.action', ampliconSizeDistribution: '/protected/designresult/downloadAmpliconSizeDistributionHistogramFile.action', validateSelectedDNAType: '/protected/design/contents/validateSelectedDNAType.action', oncomineSelect : '/protected/oncomine/oncomineSelect.action', validateDesignName: '/protected/oncomine/validateDesignName.action', createCustomOncomineDesign: '/protected/oncomine/createCustomOncomineDesign.action', submitOncomineDesignJob: '/protected/oncomine/submitOncomineDesignJob.action', validateGeneTarget: '/protected/oncomine/validateGeneTarget.action', uploadGeneTarget: '/protected/oncomine/uploadGeneTarget.action', updateDesignDetails: '/protected/oncomine/updateDesignDetails.action', orderOncominePanel: '/protected/oncomine/orderOncominePanel.action', oncomineRequestQuote: '/protected/phoenix/oligosorder/oncomineRequestQuote.action', distributorUserOrder: '/protected/oncomine/distributorUserOrder.action' , editOncomineDesignDetails: '/protected/oncomine/editOncomineDesignDetails.action', editCustomDesign: '/protected/oncomine/editCustomDesign.action', exportOncomineTargets: '/protected/oncomine/exportOncomineTargets.action', existingSharedUsers: '/protected/sharedesign/existingSharedUsers.action', createShareDesign: '/protected/sharedesign/createShareDesign.action', unSharedUser: '/protected/sharedesign/unSharedUser.action', validateSharedDesignUsers : '/protected/sharedesign/validateSharedDesignUsers.action', generateAccessCode : '/protected/useraccesscode/generateAccessCode.action', saveAccessCode : '/protected/useraccesscode/saveAccessCode.action', getReactionSizeForDesign : '/protected/designsolution/getReactionSizeForDesign.action', getReactionSizeForWgDesign : '/protected/tmpl/getReactionSizeForWgDesign.action', saveToCart : '/protected/cart/additionalProducts/save.action', requestQuote : '/protected/oligosorder/contents/save.action', orderCommunityPanel :'/protected/tmpl/orderCommunityPanel.action', consumables : '/protected/cart/additionalProducts/consumables.action', populateGenesForSelectedPanel : '/protected/oncomine/populateGenesForSelectedPanel.action', faq: '/help/faq.action', downloadOncomineResults : '/protected/designresult/downloadOncomineResults.action', createOncomineGeneSelect : '/protected/createOncomineGeneList.action', consumablesByType : '/protected/cart/additionalProducts/consumablesByType.action', createNewP4ODesign: '/protected/oncomine/createNewP4ODesign.action', deleteDNADuplicateTargets: '/protected/design/dna/contents/deleteDNADuplicateTargets.action', deleteRNADuplicateTargets: '/protected/design/rna/contents/deleteRNADuplicateTargets.action', surveyDetails: '/protected/survey/surveyDetails.action', setNeverForSurvey: '/protected/survey/setNeverForSurvey.action', setPartialSurvey: '/protected/survey/setPartialSurvey.action', panelPricing : '/protected/cart/design/skus.action', consumablesPricing : '/protected/cart/consumable/pricelist.action', exportAllTargets : '/protected/target/contents/exportAllTargets.action', exportAmplicons : '/protected/reuseamplicons/contents/downloadAmplicons.action', exportTmplTargets : '/protected/tmpl/contents/exportAllTargets.action', exportTmplAmplicons : '/protected/tmpl/reuseamplicons/contents/downloadAmplicons.action', saveSosDocument : '/protected/sosDownload/contents/save.action', finalizeAsodDesign: '/protected/inventoried/contents/finalizeDesign.action', precartSummaryData: '/protected/cart/additionalProducts/orderFormatSelection.action', getQuestions: '/spring/getQuestions', communityConsiderationResponse: '/spring/communityConsiderationResponse', downloadBlackListedAmp: '/protected/target/contents/exportAllBlackListedAmplicons.action' }; } </script> <script src="https://cdnjs.cloudflare.com/ajax/libs/Chart.js/2.4.0/Chart.min.js"></script>  <script>(function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer','GTM-T2LCHJJ8'); </script>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-CQJPJT2771"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-CQJPJT2771', { debug_mode: 'false' }); var gaTrack = function trackGoogleAnalytics(eventName, PageName){ gtag('event', eventName, { eventCategory: PageName, eventLabel: PageName, eventAction: '', eventValue: 0 }); }; </script>  <script type='text/javascript'> (function() { var f = function() { EF.init({ eventType: "pageview", pageviewProperties : "", segment : "41681", searchSegment : "", sku : "", userid : "3652", pixelHost : "pixel.everesttech.net" , allow3rdPartyPixels: 1}); EF.main(); }; window.EF = window.EF || {}; if (window.EF.main) { f(); return; } window.EF.onloadCallbacks = window.EF.onloadCallbacks || []; window.EF.onloadCallbacks[window.EF.onloadCallbacks.length] = f; if (!window.EF.jsTagAdded) { var efjs = document.createElement('script'); efjs.type = 'text/javascript'; efjs.async = true; efjs.src = 'https://www.everestjs.net/static/st.v3.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(efjs, s); window.EF.jsTagAdded=1; } })(); </script> <noscript><img src="http://pixel.everesttech.net/3652/v?" width="1" height="1"/></noscript> <meta property="og:title" content="Design custom NGS panels with Ion AmpliSeq Designer" /> <meta property="og:type" content="website" /> <meta property="og:image" content="/resources/images/Custom%20AmpliSeq%201200x628_1.png" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/assets/tether/tether.min.css" /> <link href="https://cdn.jsdelivr.net/npm/bootstrap@5.1.3/dist/css/bootstrap.min.css" rel="stylesheet" integrity="sha384-1BmE4kWBq78iYhFldvKuhfTAU6auU8tT94WrHftjDbrCEXSU1oBoqyl2QvZ6jIW3" crossorigin="anonymous"> <link type="text/css" rel="stylesheet" media="all" href="/resources/assets/dropdown/css/style.css" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/assets/animatecss/animate.min.css" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/assets/theme/css/style.css" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/assets/ampliseq/css/amp-additional.css" /> <link type="text/css" rel="stylesheet" media="all" href="/resources/assets/please-wait/please-wait.css" /> <script src="https://code.jquery.com/jquery-3.6.0.min.js" integrity="sha256-/xUj+3OJU5yExlq6GSYGSHk7tPXikynS7ogEvDej/m4=" crossorigin="anonymous"></script> <script type="text/javascript" src="/resources/assets/please-wait/please-wait.min.js?revision=v7.8.5-r06a2f73b8b"></script> <script async="async" src="//consent.truste.com/notice?domain=thermofishermicrosites.com&c=teconsent&js=nj&noticeType=bb&gtm=1&text=true" crossorigin=""></script> </head> <body class="splash-layout">  <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-T2LCHJJ8" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript>  <div id="consent_blackbar"></div> <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <script type="text/javascript"> var require = { "paths": { "datatables": "init/datatables", "datatables-raw": "lib/jquery/jquery.dataTables.min", "hbs": "hbs/hbs", "jquery": "empty:", "jquery.address": "lib/jquery/jquery.address-1.6-5am-modified", "jquery.alerts": "lib/jquery/jquery.alerts-1.1", "jquery.center": "lib/jquery/jquery.center.min", "jquery.cookie": "lib/jquery/jquery.cookie", "jquery.fileupload": "lib/jquery/jquery.fileupload", "jquery.fileupload-validate": "lib/jquery/jquery.fileupload-validate", "jquery.fileupload-process": "lib/jquery/jquery.fileupload-process", "jquery.geneGrid": "lib/geneGrid/jquery.geneGrid", "jquery.geneTable": "lib/geneTable/jquery.geneTable", "jquery.highlight": "lib/jquery/jquery.highlight", "jquery.iframe-transport": "lib/jquery/jquery.iframe-transport", "jquery.jqprint": "lib/jquery/jquery.jqprint-0.3", "jquery.maxlength": "lib/jquery/jquery.maxlength-1.0.3.pack", "jquery.msg": "lib/jquery/jquery.msg.min", "jquery.miller": "lib/miller/jquery.miller", "jquery.measure": "lib/measure/jquery.measure", "jquery.qtip": "lib/jquery/jquery.qtip.min", "jquery.select": "lib/jquery/select2", "jquery.sorted": "lib/sorted/jquery.sorted", "jquery-ui": "lib/jquery/jquery-ui-1.9.2.custom.min", "jquery.ajaxQueue": "lib/jquery/jquery.ajaxQueue.min", "json3": "lib/json3/json3.min", "lodash": "lib/lodash/lodash.min", "d3js": "lib/d3js/d3.min", "scroll": "lib/jquery/scroll", "showdown": "lib/showdown/showdown-0.2.0.min", "uuid": "lib/uuid/uuid", "igv" : "lib/igv/igv-1.0.5", "element": "angular/element" } }; window.require = { baseUrl: "/resources/js", paths: window.require["paths"], urlArgs: "build=v7.8.5-r06a2f73b8b" }; </script> <input type="hidden" id="enabledIvgnFormLogin" value="true" /> <input type="hidden" id="tfsDomain" value="row" />  <script type="text/javascript"> (function () { window.pageSpecificTexts = window.pageSpecificTexts || {}; pageSpecificTexts["login.specialCharacters.errorMessage"] = 'Special characters are currently not supported in user names.'; }()); </script> <div style="max-width: 100%;overflow-x: hidden;"> <section class="menu amp-header-section" once="menu" id="menu2-0"> <nav class="navbar navbar-expand beta-menu navbar-dropdown align-items-center navbar-fixed-top navbar-toggleable-sm bg-color transparent"> <button class="navbar-toggler navbar-toggler-right" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation"> <div class="hamburger"> <span></span> <span></span> <span></span> <span></span> </div> </button> <div class="menu-logo"> <div class="navbar-brand"> <span class="navbar-logo"> <a class="thermo-fisher-scientifc logo" href="/"> <img src=" /resources/images/thermo-fisher-logo.png " width="160" height="39"/> </a> </span> <span class="navbar-caption-wrap"><span class="navbar-caption ampliseq-caption">Ion AmpliSeq Designer</span></span> </div> </div> <div class="collapse navbar-collapse" id="navbarSupportedContent"> <ul class="navbar-nav nav-dropdown nav-right" data-app-modern-menu="true"> <li class="nav-item"> <a id="start-page-link" target="_blank" class="outer nav-link link display-5" href="https://www.thermofisher.com/ca/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-select-targets/ampliseq-target-selection/custom-panels.html" title="About Ion AmpliSeq Designer">About Ion AmpliSeq Designer</a> </li> <li class="nav-item"> <a id="help-link" class="outer nav-link link display-5" href="https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-select-targets.html" title="Ion AmpliSeq Panels">Ion AmpliSeq Panels</a> </li> <li class="nav-item"> <a id="help-link" class="outer nav-link link display-5" href="https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing.html" title="Ion Torrent NGS Solution">Ion Torrent NGS Solution</a> </li> <li class="nav-item"> <a id="help-link" class="outer nav-link link display-5" href="/help.action" title="Help">Help</a> </li> </ul> </div> </nav> </section> <section class="header3 amp-section-one" id="header3-1"> <div class="container-fluid"> <div class="row ampliseq-login-bg align-middle"> <div class="col ps-0"><div class="bg-dark bg-gradient" style="width:80%;opacity:80%;padding:3.5rem 2rem"><h2 class="m-0 text-white fs-3 fw-bold pb-3">Welcome to Ion AmpliSeq Designer</h2><h5 class="m-0 text-white fw-normal lh-base" style="opacity:70%;font-size:19px">This free online tool makes it easy to design customized AmpliSeq panels to meet your unique targeted NGS testing needs.</h5></div></div> <div class="col-sm-8 col-md-6 col-lg-4 my-auto me-4"> <div id="secure" class="thermo-login text-center"> <div class="banner-info-container"> <span class="col-sm-1 col-md-1 col-lg-1 ps-0"><img class="bannerIcon" src="/resources/images/lightbulbTipsandTricks.svg"/></span> <span class="col-sm-11 col-md-11 col-lg-11 banner-info-message"><div class="lh-base">Want to save time and money getting your NGS tests up and running? Our <a href="http://www.thermofisher.com/av" target="_blank" class="">analytical validation (AV) consulting services </a> can help! Have an <a href="https://www.thermofisher.com/us/en/home/global/forms/qualification-validation-calibration-services.html" target="_blank" class="">AV specialist</a> contact you.<p class="banner-color">(availability and levels of service may vary by region)</p></div></span> </div> <div id="loginIE" class="banner-info-container"> <span class="banner-info-message"><span class="d-flex mb-2" style="margin-top: -4px;"><label class="badge badge-primary mt-0">New!</label> <span class="ps-2">The Ion AmpliSeq™ Liverpool Lymphoid Network Panel is now available in a small pack format. Check ordering options on panel page after login.</span></span></span> </div> <h2 class="amp-section-title pb-3 amp-fonts-style">Log in to start a design</h2> <div class="control-group form-wrapper pb-3"> <div class="controls"> <a id="signInButton" class="btn-featured btn-lg" href='#'> Sign In </a> </div> </div> <div class="create-account"> <a id="createAccount" href="#"> Create new account  </a> <a id="troubleSignIn" href="#"> Trouble Signing In  </a> </div> <div class="loginLocation" style="height: 35px;"> <div class="location-selection" style="float:right" data-bs-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> <img id="login-globe-icon" width="40px" height="40px" class="login-icon hideElement login-globe-icon" data-bs-toggle="tooltip" title="Click to change login domain" src="/resources/images/login-globe.png"> <img id="login-china-icon" width="40px" height="40px" class="login-icon hideElement login-china-icon" data-bs-toggle="tooltip" title="Click to change login domain" src="/resources/images/login-china.png"> </div> <div class="dropdown-menu"> <div style="padding-left: 10px"> Select Login Domain </div> <div class="dropdown-item option-globe" data-login="row""> <img class="tick-mark hidden" width="15px" height="10px" src="/resources/images/checkmark_rna.png"> <img width="30px" height="30px" src="/resources/images/login-globe.png"><span> www.thermofisher.com </span> </div> <div class="dropdown-item option-china" data-login="china"> <img class="tick-mark hidden" width="15px" height="10px" src="/resources/images/checkmark_rna.png"> <img width="30px" height="30px" src="/resources/images/login-china.png"><span> www.thermofisher.cn </span> </div> </div> </div> </div> </div> </div> <div class="mt-4"> <div class="p-4 row"> <div class="col-lg-6 col-md-6 col-sm-8"> <h2 class="fw-bold mt-0 lh-base">How Ion AmpliSeq Targeted Next-Generation Sequencing Technology Works</h2> <h5 class="lh-base fw-normal text-left mt-3" style="font-size: 19px;"> Ion AmpliSeq NGS panels transform clinical and genetic research with ultra high-multiplex PCR primer pools designed for fast and simple production of tens to thousands of targeted amplicons from samples containing as little as 1ng of input DNA, such as formalin-fixed, paraffin-embedded (FFPE) and fine needle biopsy (FNB) research samples. </h5> <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-select-targets/ampliseq-target-selection/how-ampliseq-technology-works.html" target="_blank" class="fw-bold btn btn-primary mt-3 mx-0 cta-buttons" style="font-size: 17px;" > Learn more about how AmpliSeq Works </a> </div> <div class="text-center col"> <iframe height="350" src="https://players.brightcove.net/3663210762001/default_default/index.html?videoId=5030839650001" title="AmpliSeq Overview Video" width="100%" allowFullScreen="true"></iframe> <h3 class="mt-4" style="color: #ee3134; font-size: 17px;"><i class="align-middle fa-circle-play fa-solid fs-4"></i> Watch AmpliSeq Overview Video</h3> </div> </div> <div class="p-4 row"> <h2 class="fw-bold text-center m-0">Benefits of Ion AmpliSeq Technology</h2> <div class="text-center mt-4 row"> <div class="col-lg-4"> <img alt="Small Sample Input" src="/resources/images/eppendorf-tube.png" class="" /> <h4 style="color: #ee3134;" class="fw-bold fs-5">Small Sample Input</h4> <h5 class="lh-base fw-light px-5 py-2" style="font-size: 17px;">Low DNA or RNA input requirement for targeted NGS to maximize test success rates and minimize "quantity not sufficient" (QNS) results</h5> </div> <div class="col-lg-4"> <img alt="Scalable & Customizable Panels" src="/resources/images/plate-protein-analysis.png" class="" /> <h4 style="color: #ee3134;" class="fw-bold fs-5">Scalable & Customizable Panels</h4> <h5 class="lh-base fw-light px-5 py-2" style="font-size: 17px;">One to hundreds of gene targets in a single run with predesigned or custom panels so you can develop the right test for your application</h5> </div> <div class="col-lg-4"> <img alt="Fast Automated Workflow" src="/resources/images/gears-scheduled-maintenance.png" class="" /> <h4 style="color: #ee3134;" class="fw-bold fs-5">Fast Automated Workflow</h4> <h5 class="lh-base fw-light px-5 py-2" style="font-size: 17px;">From sample to data in as little as 24 hours, with just 10-45 minutes of hands-on time so you can rapidly and easily receive your NGS test results</h5> </div> </div> <div class="text-center"> <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-select-targets/ampliseq-target-selection.html" target="_blank" class="fw-bold text-center btn btn-primary mt-4 w-auto cta-buttons" style="font-size: 17px;" > Explore more benefits of Ion AmpliSeq </a> </div> </div> <div class="p-4 row"> <h2 class="fw-bold text-center">Ion AmpliSeq Technology Applications</h2> <div class="row my-4"> <div class="col-lg-3"> <div class="text-center p-4" style="background: #dfe0e2; height: 25rem;"> <div style="height: 9rem;"> <a href="https://www.thermofisher.com/us/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology.html" target="_blank" class=""> <img alt="Cancer Research" src="/resources/images/cancer-research.png" width="90" class="" /> </a> </div> <h4 style="height: 4rem;" class=""> <a href="https://www.thermofisher.com/us/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology.html" target="_blank" class="fw-bold text-center fs-5 tfs-anchor-red"> Cancer Research </a> </h4> <h5 class="lh-base fw-light" style="height: 4rem; font-size: 17px;"> Cancer genomics, cancer RNA expression, and more <p class=""></p> </h5> <a href="https://www.thermofisher.com/us/en/home/clinical/preclinical-companion-diagnostic-development/oncomine-oncology.html" target="_blank" class="fw-bold text-center" style="font-size: 17px;">Learn More</a> </div> </div> <div class="col-lg-3"> <div class="text-center p-4" style="background: #dfe0e2; height: 25rem;"> <div style="height: 9rem;"> <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-applications/applications-next-generation-sequencing-inherited-disease-research.html" target="_blank" class="" > <img alt="Inherited Disease Research" src="/resources/images/inherited-diseases.png" class="" /> </a> </div> <h4 style="height: 4rem;" class=""> <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-applications/applications-next-generation-sequencing-inherited-disease-research.html" target="_blank" class="fw-bold text-center fs-5 tfs-anchor-red" > Inherited Disease Research </a> </h4> <h5 class="lh-base fw-light" style="height: 4rem; font-size: 17px;">Deafness, epilepsy, cardiac research, and more</h5> <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-applications/applications-next-generation-sequencing-inherited-disease-research.html" target="_blank" class="fw-bold text-center" style="font-size: 17px;" > Learn More </a> </div> </div> <div class="col-lg-3"> <div class="text-center p-4" style="background: #dfe0e2; height: 25rem;"> <div style="height: 9rem;"> <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/dna-sequencing/microbial-sequencing/microbial-identification-ion-torrent-next-generation-sequencing.html" target="_blank" class="fw-bold text-center" > <img alt="Infectious Disease and Microbiology Research" src="/resources/images/viruses.png" class="" /> </a> </div> <h4 style="height: 4rem;" class=""> <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/dna-sequencing/microbial-sequencing/microbial-identification-ion-torrent-next-generation-sequencing.html" target="_blank" class="fw-bold text-center fs-5 tfs-anchor-red" style="line-height: 27px; margin-top: 6px;" > Infectious Disease and Microbiology Research </a> </h4> <h5 class="lh-base fw-light" style="height: 4rem; font-size: 17px;">Tracing the origin of outbreaks, food safety and more</h5> <a href="https://www.thermofisher.com/us/en/home/life-science/sequencing/dna-sequencing/microbial-sequencing/microbial-identification-ion-torrent-next-generation-sequencing.html" target="_blank" class="fw-bold text-center" style="font-size: 17px;" > Learn More </a> </div> </div> <div class="col-lg-3"> <div class="text-center p-4" style="background: #dfe0e2; height: 25rem;"> <div style="height: 9rem;"> <a href="https://www.thermofisher.com/us/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis/next-generation-sequencing-ngs-forensics.html" target="_blank" class=""> <img alt="Human Identification" src="/resources/images/design-sequence.png" class="" /> </a> </div> <h4 style="height: 4rem;" class=""> <a href="https://www.thermofisher.com/us/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis/next-generation-sequencing-ngs-forensics.html" target="_blank" class="fw-bold text-center fs-5 tfs-anchor-red" > Human Identification </a> </h4> <h5 class="lh-base fw-light" style="height: 4rem; font-size: 17px;">DNA phenotyping, ancestry, and more</h5> <a href="https://www.thermofisher.com/us/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis/next-generation-sequencing-ngs-forensics.html" target="_blank" class="fw-bold text-center" style="font-size: 17px;" > Learn More </a> </div> </div> </div> </div> <div class="row mb-3 px-4"> <h2 class="fw-bold">Get Started with Ion AmpliSeq</h2> <h5 class="lh-base fw-normal text-left my-2 pe-4" style="font-size: 19px;"> From predesigned to customizable research panels, all our content is available at AmpliSeq.com. Find in-depth descriptions of each predesigned panel, including gene lists and any relevant publications. Or access Ion AmpliSeq Designer, our easy yet powerful custom design tool, and modify any predesigned panel or create your own design <i>de novo</i>. There is no charge to access content or try out the design tool. </h5> <h5 class="lh-base fw-normal text-left m-0" style="font-size: 19px;">Get started by logging in or creating your account above. Have questions? Visit our <a href="/help.action" target="_blank" class="fw-bold">Help Center</a>.</h5> </div> </div> </div> </section> <script type="text/javascript" src="/resources/assets/tether/tether.min.js?revision=v7.8.5-r06a2f73b8b"></script> <script src="https://cdn.jsdelivr.net/npm/bootstrap@5.1.3/dist/js/bootstrap.bundle.min.js" integrity="sha384-ka7Sk0Gln4gmtz2MlQnikT1wXgYsOg+OMhuP+IlRH9sENBO0LRn5q+8nbTov4+1p" crossorigin="anonymous"></script> <script type="text/javascript" src="/resources/assets/smoothscroll/smooth-scroll.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/dropdown/js/script.min.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/touchswipe/jquery.touch-swipe.min.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/parallax/jarallax.min.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/amp-tabs/amp-tabs.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/ytplayer/jquery.mb.ytplayer.min.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/vimeoplayer/jquery.mb.vimeo_player.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/viewportchecker/jquery.viewportchecker.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/theme/js/script.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src="/resources/assets/slidervideo/script.js?revision=v7.8.5-r06a2f73b8b"></script> <script type="text/javascript" src=" /resources/js/lib/require-jquery.js?build=v7.8.5-r06a2f73b8b "></script> <script type="text/javascript" src="/resources/js/login.js?revision=v7.8.5-r06a2f73b8b"></script> <script> (function () { window.pageSpecificTexts = window.pageSpecificTexts || {}; pageSpecificTexts["login.specialCharacters.errorMessage"] = 'Special characters are currently not supported in user names.'; }()); </script> <input name="animation" type="hidden"> <div class="modal" id="aboutAmpliseq" data-bs-backdrop="static" data-bs-keyboard="false" tabindex="-1"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <h2 class="modal-title" id="modalLabel">Welcome to Ion AmpliSeq Designer </h2> <button type="button" class="close" data-bs-dismiss="modal" aria-label="Close"> <span aria-hidden="true">×</span> </button> </div> <div class="modal-body"> From predesigned to customizable DNA and RNA panels for next-generation sequencing (NGS), all of our content is available within the Ion AmpliSeq Designer (including in-depth descriptions and gene lists for each panel). This custom design tool lets you modify any predesigned panel or create your own design de novo. There is no charge to access content or try out the tool. </div> <div class="modal-footer"> <button type="button" id="aboutAmpliseqCloseBtn" class="btn btn-secondary btn-sm" data-bs-dismiss="modal">Close</button> </div> </div> </div> </div> </div> <script type="text/javascript" src="/resources/js/footer.js?revision=v7.8.5-r06a2f73b8b"></script> <div id="footer" class="text-center"> <ul class="footer-links inline-line-items" style="margin:0px;"> <li><a href="/terms.jsp" onclick="gaTrack('Footer: T&C Link Clk','Ion Ampliseq Designer')">Terms & Conditions</a></li> <li><a href="https://www.thermofisher.com/us/en/home/global/privacy-policy.html" target="_blank" onclick="gaTrack('Footer: Privacy Policy Link Clk','Ion Ampliseq Designer')">Privacy Policy</a></li> <li><a href="/help.action" onclick="gaTrack('Footer: Help Link Clk','Ion Ampliseq Designer')">Help</a></li> <li><a href="https://www.thermofisher.com/us/en/home/technical-resources/contact-us.html" target="_blank" onclick="gaTrack('Footer: Reg Cont Link Clk','Ion Ampliseq Designer')">Regional Contact</a></li> <li><a target="_blank" href="http://www.thermofisher.com/us/en/home/technical-resources/order-support.html" onclick="gaTrack('Footer: T&O Support Link Clk','Ion Ampliseq Designer')">Technical & Order Support</a></li> <li> <a href="#assay-design-modal-login" data-bs-toggle="modal" id="assay-design-footer-login" onclick="gaTrack('Footer: Assay Dsg Support Clk','Ion Ampliseq Designer')">Assay Design Support</a> </li> <li><a id="change-log" href="/displayChangeLog.action" onclick="gaTrack('Footer: Release Noteslp Clk','Ion Ampliseq Designer')">Release Notes</a></li> <li><div id="teconsent" class="d-inline-block"></div></li> </ul> <div class="disclaimer font-weight-normal" style="font-size:12px">For Research Use Only. Not for use in diagnostic procedures.</div> <div class="legal-text font-weight-normal" style="font-size:12px">© <span id="copyright-year"></span> Thermo Fisher Scientific. All Rights Reserved. Ion AmpliSeq Designer v7.8.5 <span style="display:none" id="ampliseqRevision">r1729150848</span></div> <div id="assay-design-modal-login" class="modal text-left" data-bs-backdrop="static" data-bs-keyboard="false" tabindex="-1" role="dialog"> <div class="modal-dialog" role="document"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close assay-design-modal-close float-right" data-bs-dismiss="modal" aria-hidden="true">×</button> <h3 id="myModalLabel">Assay Design Support</h3> </div> <div class="modal-body"> <div class="assay-design-modal-title">Choose email based on you region:</div> <li><a href="mailto:abtechsupport@thermofisher.com">Americas</a></li> <li><a href="mailto:ngs-eusupport@thermofisher.com">EMEA</a></li> <li><a href="mailto:ngs-gcsupport@thermofisher.com">Greater China</a></li> <li><a href="mailto:ngs-sasiasupport@thermofisher.com">South Asia</a></li> <li><a href="mailto:jptech@thermofisher.com">Japan</a></li> </div> <div class="modal-footer"> <a href="#" class="btn btn-sm btn-outline-secondary assay-design-modal-close" data-bs-dismiss="modal">Close</a> </div> </div> </div> </div> </div> <a href="javascript:" class="return-to-top"><i class="fa fa-angle-double-up"></i></a> </body> </html>

CINXE.COM

Design custom NGS panels with Ion AmpliSeq Designer