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Search results for: organochlorine

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text-center" style="font-size:1.6rem;">Search results for: organochlorine</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">31</span> Plants and Microorganisms for Phytoremediation of Soils Polluted with Organochlorine Pesticides</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maritsa%20Kurashvili">Maritsa Kurashvili</a>, <a href="https://publications.waset.org/abstracts/search?q=George%20Adamia"> George Adamia</a>, <a href="https://publications.waset.org/abstracts/search?q=Tamar%20Ananiashvili"> Tamar Ananiashvili</a>, <a href="https://publications.waset.org/abstracts/search?q=Lia%20Amiranasvili"> Lia Amiranasvili</a>, <a href="https://publications.waset.org/abstracts/search?q=Tamar%20Varazi"> Tamar Varazi</a>, <a href="https://publications.waset.org/abstracts/search?q=Marina%20Pruidze"> Marina Pruidze</a>, <a href="https://publications.waset.org/abstracts/search?q=Marlen%20Gordeziani"> Marlen Gordeziani</a>, <a href="https://publications.waset.org/abstracts/search?q=Gia%20Khatisashvili"> Gia Khatisashvili</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The goal of presented work is the development phytoremediation method targeted to cleaning environment polluted with organochlorine pesticides, based on joint application of plants and microorganisms. For this aim the selection of plants and microorganisms with corresponding capabilities towards three organochlorine pesticides (Lindane, DDT and PCP) has been carried out. The tolerance of plants to tested pesticides and induction degree of plant detoxification enzymes by these compounds have been used as main criteria for estimating the applicability of plants in proposed technology. Obtained results show that alfalfa, maize and soybean among tested six plant species have highest tolerance to pesticides. As a result of screening, more than 30 strains from genera Pseudomonas have been selected. As a result of GC analysis of incubation area, 11 active cultures for investigated pesticides are carefully chosen. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DDT" title="DDT">DDT</a>, <a href="https://publications.waset.org/abstracts/search?q=Lindane" title=" Lindane"> Lindane</a>, <a href="https://publications.waset.org/abstracts/search?q=organochlorine%20pesticides" title=" organochlorine pesticides"> organochlorine pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=PCP" title=" PCP"> PCP</a>, <a href="https://publications.waset.org/abstracts/search?q=phytoremediation" title=" phytoremediation"> phytoremediation</a> </p> <a href="https://publications.waset.org/abstracts/3753/plants-and-microorganisms-for-phytoremediation-of-soils-polluted-with-organochlorine-pesticides" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3753.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">315</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">30</span> Use of Fabric Phase Sorptive Extraction with Gas Chromatography-Mass Spectrometry for the Determination of Organochlorine Pesticides in Various Aqueous and Juice Samples</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ramandeep%20Kaur">Ramandeep Kaur</a>, <a href="https://publications.waset.org/abstracts/search?q=Ashok%20Kumar%20Malik"> Ashok Kumar Malik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fabric Phase Sorptive Extraction (FPSE) combined with Gas chromatography Mass Spectrometry (GCMS) has been developed for the determination of nineteen organochlorine pesticides in various aqueous samples. The method consolidates the features of sol-gel derived microextraction sorbents with rich surface chemistry of cellulose fabric substrate which could directly extract sample from complex sample matrices and incredibly improve the operation with decreased pretreatment time. Some vital parameters such as kind and volume of extraction solvent and extraction time were examinedand optimized. Calibration curves were obtained in the concentration range 0.5-500 ng/mL. Under the optimum conditions, the limits of detection (LODs) were in the range 0.033 ng/mL to 0.136 ng/mL. The relative standard deviations (RSDs) for extraction of 10 ng/mL 0f OCPs were less than 10%. The developed method has been applied for the quantification of these compounds in aqueous and fruit juice samples. The results obtained proved the present method to be rapid and feasible for the determination of organochlorine pesticides in aqueous samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fabric%20phase%20sorptive%20extraction" title="fabric phase sorptive extraction">fabric phase sorptive extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography-mass%20spectrometry" title=" gas chromatography-mass spectrometry"> gas chromatography-mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=organochlorine%20pesticides" title=" organochlorine pesticides"> organochlorine pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=sample%20pretreatment" title=" sample pretreatment"> sample pretreatment</a> </p> <a href="https://publications.waset.org/abstracts/80494/use-of-fabric-phase-sorptive-extraction-with-gas-chromatography-mass-spectrometry-for-the-determination-of-organochlorine-pesticides-in-various-aqueous-and-juice-samples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80494.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">484</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">29</span> Comparative Assessment of Organo-Chlorine Pesticides Residue in Fruits and Fruit Juices</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saidu%20Garba%20Okereafor%20Stella">Saidu Garba Okereafor Stella</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The presence of 15 organochlorine pesticides residue was assessed from 29 different fruits and fruit juice samples from selected farms in Kaduna and Niger States using the quick easy cheap effective rugged and safe (QuEChERS), followed by gas chromatography-tandem mass spectrometry (GC-MS/MS). The results showed the presence of varying concentrations of ten (10) organochlorine pesticide residues in all the samples with Endrin ketone showing the highest concentration in 3 samples from Kaduna (guava juice 1 and 2 0.099 to 0.145 mg/kg) and Niger States (orange juice J19 0.102 mg/kg). The heptachlor was detected at high concentration in 11 samples, 7 samples from Kaduna State (mango juice 0.011 mg/kg, Washington orange 0.014 mg/kg, Valencia orange fruit 0.020 mg/kg, orange juice 0.011, white guava fruit 0.024 mg/kg, guava juice 0.023 mg/kg, guava juice 2 0.024 mg/kg) and 4 samples from (mango juice 1 0.015 mg/kg, pineapple juice 1 0.0120 mg/kg pineapple juice 2 011 mg/kg and mix juice 2 0.012 mg/kg) from Niger State. Dieldrine and endosulfansulfate were detected at high levels in one sample each from Niger (guava fruit 0.019 mg/kg and mixed juice1 0.011mg/kg), respectively. However, all were above the maximum residue limits (MRLs) set by WHO/FAO which suggest that people consuming these type of contaminated fruits and fruits juices may contact diseases associated with those organochlorine pesticides residue. Minute concentrations of other organochlorines (α- BHC, δ- BHC, β- BHC, Lindane, and p’p DDT) ranged from 0.003 to 0.015 were recorded below the MRLs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fruits%20and%20fruits%20juices" title="fruits and fruits juices">fruits and fruits juices</a>, <a href="https://publications.waset.org/abstracts/search?q=organochlorine%20pesticide%20residue" title=" organochlorine pesticide residue"> organochlorine pesticide residue</a>, <a href="https://publications.waset.org/abstracts/search?q=comparative%20studies" title=" comparative studies"> comparative studies</a>, <a href="https://publications.waset.org/abstracts/search?q=gc-ms%20spectrophometer" title=" gc-ms spectrophometer"> gc-ms spectrophometer</a> </p> <a href="https://publications.waset.org/abstracts/136919/comparative-assessment-of-organo-chlorine-pesticides-residue-in-fruits-and-fruit-juices" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/136919.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">147</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28</span> Organochlorine and Organophosphorus Pesticide Residues in Fish Samples from Lake Chad, Baga, North Eastern Nigeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=J.%20C.%20Akan">J. C. Akan</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20I.%20%20Abdulrahman"> F. I. Abdulrahman</a>, <a href="https://publications.waset.org/abstracts/search?q=Z.%20M.%20Chellube"> Z. M. Chellube</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this study was to determine the levels of some organochlorine (o, p-DDE, p,p’-DDD, o,p’-DDD, p,p’-DDT, p,p’-DDT, α-BHC, γ-BHC, lindane, Endosulfan sulphate, dieldrin and aldrin and organophosphorus (Dichlorvos, Diazinon, Chlorpyrifos, fenitrothion and Fenitrothion) pesticide residues in the gills, liver, stomach, kidney and flesh of four fish species (Tilapia zilli, Clarias anguillaris Hetrotis niloticus and Oreochronmis niloticus) between the periods of September 2010 to October, 2011. Samples were collected from Kwantan turare in Lake Chad, Baga, Borno State, Nigeria. Extraction of the fish samples and de-fattening of the fish sample extracts were performed using standard procedures. Analysis of the fish samples for pesticide residues were carried out using Shimadzu GC/MS (GC – 17A), equipped with fluorescence detector. Large differences in the levels of pesticide residues were observed between tissues within each fish. The concentrations of all the organophosphorus pesticides were higher in the organs of Oreochronmis niloticus, while Hetrotis niloticus shows the lowest. For organochlorine pesticides, the organs of Tilapia zilli showed the highest concentrations, while Hetrotis niloticus shows the lowest. The highest pesticide concentrations were observed in gills and liver tissues of all the species of fish study, while the lowest concentrations were observed in flesh. Based on the above results, it can therefore be concluded that the concentrations of pesticide in the four fish species study did exceed the permissible limits set by FAO and FEPA. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=organochlorine" title="organochlorine">organochlorine</a>, <a href="https://publications.waset.org/abstracts/search?q=organophosphorus" title=" organophosphorus"> organophosphorus</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticides" title=" pesticides"> pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=accumulation" title=" accumulation"> accumulation</a>, <a href="https://publications.waset.org/abstracts/search?q=fish" title=" fish"> fish</a>, <a href="https://publications.waset.org/abstracts/search?q=lake%20chad" title=" lake chad"> lake chad</a> </p> <a href="https://publications.waset.org/abstracts/1908/organochlorine-and-organophosphorus-pesticide-residues-in-fish-samples-from-lake-chad-baga-north-eastern-nigeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/1908.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">700</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27</span> Persistent Organochlorine Pesticides (POPs) in Water, Sediment, Fin Fishes (Schilbes mystus and Hemichromis fasciatus) from River Ogun, Lagos, Nigeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Edwin%20O.%20Clarke">Edwin O. Clarke</a>, <a href="https://publications.waset.org/abstracts/search?q=Akintade%20O.%20Adeboyejo"> Akintade O. Adeboyejo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Intensive use of pesticides resulted in dispersal of pollutants throughout the globe. This study was carried out to investigate persistent Organochlorine pesticides (POPs) in water, sediment and fin fishes, Schilbes mystus and Hemichromis fasciatus from two different sampling stations along River Ogun between the month of June 2012 and January 2013. The Organochlorine pesticides analyzed include DDT (pp’1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane), DDD, DDE (pp1,1-dichloro-2, 2-bis-(4-chlorophenyl) ethylene, HCH (gamma 1,2,3,4,5,6-hexachlorocylohexane, HCB hexachlorobenzene),Dieldrin (1,2,3,4,10,10-hexachloro-6,7-epoxy-1,4,4a,5,6,7,8,8a octahydro- 1,4,5,8 dimethanonaphthalene). The analysis was done using Gas Chromatograph with Electron Capture Detector. In water sample, the result showed that PPDDT, Endrin aldehyde, Endrin ketone concentrations were high in both stations. The mean value of Organochlorine analyzed in water range from Beta BHC (0.50±0.10µg/l) to PP DDT (162.86±0.21µg/l) in Kara sample station and Beta BHC (0.20±0.07µg/l) to Endrin Aldehyde (76.47±0.02µg/l) in Odo-Ogun sample station. The levels of POPs obtained in sediments ranged from 0.40±0.23µg/g (Beta BHC) to 259.90 ± 1.00µg/kg (Endosulfan sulfate) in Kara sample station and 0.64±0.00µg/g (Beta BHC) to 379.77 ±0.15 µg/g (Endosulfan sulfate) in Odo-Ogun sample station. The levels of POPs obtained in fin fish samples ranged from 0.29±0.00µg/g (Delta BHC) to 197.87 ± 0.31µg/g (PP DDT) in Kara sample station and in Odo-Ogun sample station the mean value for fish samples range from 0.29 ± 0.00 µg/g (Delta BHC) to 197.87 ± 0.32 µg/g (PP DDT). The study showed that the accumulation of POPs affect the environment and reduce water quality. The results showed that the concentrations were found to exceed the maximum acceptable concentration of 0.10µg/l value set by the European Union for the protection of freshwater aquatic life and this can be hazardous if the trend is not checked. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hazardous" title="hazardous">hazardous</a>, <a href="https://publications.waset.org/abstracts/search?q=persistent" title=" persistent"> persistent</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticides" title=" pesticides"> pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=biomes" title=" biomes"> biomes</a> </p> <a href="https://publications.waset.org/abstracts/38036/persistent-organochlorine-pesticides-pops-in-water-sediment-fin-fishes-schilbes-mystus-and-hemichromis-fasciatus-from-river-ogun-lagos-nigeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/38036.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">291</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">26</span> Organochlorine Residues in Cuttlefish from the Arabian Gulf</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20El-Gendy">A. El-Gendy</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Al-Farraj"> S. Al-Farraj</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Al%20Kahtani"> S. Al Kahtani</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20El-Hedeny"> M. El-Hedeny</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Contaminations of persistent organic pollutants (POPs) such as, dichlorodiphenyl trichloroethane (DDT), hexachlorocyclohexane (HCH) and chlordane (CHLs) were examined in the edible mantle tissues of the commercial cuttlefish Sepia pharaonis Ehrenberg 1831, collected from the marine water of the Arabian Gulf. The mean concentrations of DDT, CHLs and HCH were in the ranges of 29.4 - 56 ng/g, 47.4 - 100 ng/g and 1 - 4 ng/g, respectively. Among the POPs analyzed, HCH showed the lowest concentrations ranging between 1 to 5 ng/g lipid wt. However, concentrations of DDT, CHLs and HCH, detected in this study, were generally comparable or lower than those found in studies of similar cephalopod species from other areas subject to a high anthropogenic impact. Relationships between total body lengths and/or dorsal mantle lengths of the organisms and the concentration values of the studied POPs were also considered. Compared with recommendations of the international organizations, there are no potential risks associated with consuming the studied cuttlefish species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cuttlefish" title="cuttlefish">cuttlefish</a>, <a href="https://publications.waset.org/abstracts/search?q=Sepia%20pharaonis" title=" Sepia pharaonis"> Sepia pharaonis</a>, <a href="https://publications.waset.org/abstracts/search?q=organochlorine" title=" organochlorine"> organochlorine</a>, <a href="https://publications.waset.org/abstracts/search?q=DDT" title=" DDT"> DDT</a>, <a href="https://publications.waset.org/abstracts/search?q=CHLs" title=" CHLs"> CHLs</a>, <a href="https://publications.waset.org/abstracts/search?q=HCH" title=" HCH"> HCH</a>, <a href="https://publications.waset.org/abstracts/search?q=Arabian%20Gulf" title=" Arabian Gulf"> Arabian Gulf</a> </p> <a href="https://publications.waset.org/abstracts/1909/organochlorine-residues-in-cuttlefish-from-the-arabian-gulf" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/1909.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">384</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">25</span> Application of Aquatic Plants for the Remediation of Organochlorine Pesticides from Keenjhar Lake</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Soomal%20Hamza">Soomal Hamza</a>, <a href="https://publications.waset.org/abstracts/search?q=Uzma%20Imran"> Uzma Imran</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Organochlorine pesticides bio-accumulate into the fat of fish, birds, and animals through which it enters the human food cycle. Due to their persistence and stability in the environment, many health impacts are associated with them, most of which are carcinogenic in nature. In this study, the level of organochlorine pesticides has been detected in Keenjhar Lake and remediated using Rhizoremediation technique. 14 OC pesticides namely, Aldrin, Deldrin, Heptachlor, Heptachlor epoxide, Endrin, Endosulfun I and II, DDT, DDE, DDD, Alpha, Beta, Gamma BHC and two plants namely, Water Hyacinth and Slvinia Molesta were used in the system using pot experiment which processed for 11 days. A consortium was inoculated in both plants to increase its efficiency. Water samples were processed using liquide-liquid extraction. Sediments and roots samples were processed using Soxhlet method followed by clean-up and Gas Chromatography. Delta-BHC was the predominantly found in all samples with mean concentration (ppb) and standard deviation of 0.02 ± 0.14, 0.52 ± 0.68, 0.61 ± 0.06, in Water, Sediments and Roots samples respectively. The highest levels were of Endosulfan II in the samples of water, sediments and roots. Water Hyacinth proved to be better bioaccumulaor as compared to Silvinia Molesta. The pattern of compounds reduction rate by the end of experiment was Delta-BHC>DDD > Alpha-BHC > DDT> Heptachlor> H.Epoxide> Deldrin> Aldrin> Endrin> DDE> Endosulfun I > Endosulfun II. Not much significant difference was observed between the pots with the consortium and pots without the consortium addition. Phytoremediation is a promising technique, but more studies are required to assess the bioremediation potential of different aquatic plants and plant-endophyte relationship. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aquatic%20plant" title="aquatic plant">aquatic plant</a>, <a href="https://publications.waset.org/abstracts/search?q=bio%20remediation" title=" bio remediation"> bio remediation</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography" title=" gas chromatography"> gas chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=liquid%20liquid%20extraction" title=" liquid liquid extraction "> liquid liquid extraction </a> </p> <a href="https://publications.waset.org/abstracts/124344/application-of-aquatic-plants-for-the-remediation-of-organochlorine-pesticides-from-keenjhar-lake" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/124344.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">149</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">24</span> Determination of Some Organochlorine Pesticide Residues in Vegetable and Soil Samples from Alau Dam and Gongulong Agricultural Sites, Borno State, North Eastern Nigeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Joseph%20Clement%20Akan">Joseph Clement Akan</a>, <a href="https://publications.waset.org/abstracts/search?q=Lami%20Jafiya"> Lami Jafiya</a>, <a href="https://publications.waset.org/abstracts/search?q=Zaynab%20Muhammad%20Chellube"> Zaynab Muhammad Chellube</a>, <a href="https://publications.waset.org/abstracts/search?q=Zakari%20Mohammed"> Zakari Mohammed</a>, <a href="https://publications.waset.org/abstracts/search?q=Fanna%20Inna%20Abdulrahman"> Fanna Inna Abdulrahman</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Five vegetables (spinach, lettuce, cabbage, tomato, and onion) were freshly harvested from the Alau Dam and Gongulong agricultural areas for the determination of some organochlorine pesticide residues (o, p-DDE, p,p’-DDD, o,p’-DDD, p,p’-DDT, α-BHC, γ-BHC, metoxichlor, lindane, endosulfan dieldrin, and aldrin.) Soil samples were also collected at different depths for the determination of the above pesticides. Samples collection and preparation were conducted using standard procedures. The concentrations of all the pesticides in the soil and vegetable samples were determined using GC/MS SHIMADZU (GC-17A) equipped with electron capture detector (ECD). The highest concentration was that of p,p’-DDD (132.4±13.45µg/g) which was observed in the leaf of cabbage, while the lowest concentration was that of p,p’-DDT (2.34µg/g) was observed in the root of spinach. Similar trends were observed at the Gongulong agricultural area, with p,p’-DDD having the highest concentration of 153.23µg/g in the leaf of cabbage, while the lowest concentration was that of p,p’-DDT (12.45µg/g) which was observed in the root of spinach. α-BHC, γ-BHC, Methoxychlor, and lindane were detected in all the vegetable samples studied. The concentrations of all the pesticides in the soil samples were observed to be higher at a depth of 21-30cm, while the lowest concentrations were observed at a depth of 0-10cm. The concentrations of all the pesticides in the vegetables and soil samples from the two agricultural sites were observed to be at alarming levels, much higher than the maximum residue limits (MRLs) and acceptable daily intake values (ADIs) .The levels of the pesticides observed in the vegetables and soil samples investigated, are of such a magnitude that calls for special attention and laws to regulate the use and circulation of such chemicals. Routine monitoring of pesticide residues in these study areas is necessary for the prevention, control and reduction of environmental pollution, so as to minimize health risks. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alau%20Dam" title="Alau Dam">Alau Dam</a>, <a href="https://publications.waset.org/abstracts/search?q=gongulong" title=" gongulong"> gongulong</a>, <a href="https://publications.waset.org/abstracts/search?q=organochlorine" title=" organochlorine"> organochlorine</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticide%20residues" title=" pesticide residues"> pesticide residues</a>, <a href="https://publications.waset.org/abstracts/search?q=soil" title=" soil"> soil</a>, <a href="https://publications.waset.org/abstracts/search?q=vegetables" title=" vegetables"> vegetables</a> </p> <a href="https://publications.waset.org/abstracts/1905/determination-of-some-organochlorine-pesticide-residues-in-vegetable-and-soil-samples-from-alau-dam-and-gongulong-agricultural-sites-borno-state-north-eastern-nigeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/1905.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">284</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">23</span> Enhanced Degradation of Endosulfan in Soil Using Lycopersicon esculentum L. (Tomato) and Endosulfan Tolerant Bacterium Strains</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rupa%20Rani">Rupa Rani</a>, <a href="https://publications.waset.org/abstracts/search?q=Vipin%20Kumar"> Vipin Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Endosulfan, an organochlorine pesticide is of environmental concern due to its apparent persistence and toxicity. It has been reported as contaminants in soil, air, and water and is bioaccumulated and magnified in ecosystems. The combined use of microorganisms and plants has great potential for remediating soil contaminated with organic compounds such as pesticides. The objective of this study was to evaluate whether the bacterial inoculation influences plant growth promotion, endosulfan degradation in soil and endosulfan accumulation in different plant parts. Lycopersicon esculentum L. (Tomato) was grown in endosulfan spiked soil and inoculated with endosulfan tolerant bacterial strains. Endosulfan residues from different parts of plants and soil were extracted and estimated by using gas chromatograph equipped with 63Ni electron capture detector (GC-ECD). The inoculation of bacterial strains into the soil with plants showed a beneficial effect on endosulfan degradation and plant biomass production. Maximum endosulfan (90%) degradation was observed after 120 days of bacterial inoculation in the soil. Furthermore, there was significantly less endosulfan accumulation in roots and shoots of bacterial strains inoculated plants as compared to uninoculated plants. The results show the effectiveness of inoculated endosulfan tolerant bacterial strains to increase the remediation of endosulfan contaminated soil. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=organochlorine%20pesticides" title="organochlorine pesticides">organochlorine pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=endosulfan" title=" endosulfan"> endosulfan</a>, <a href="https://publications.waset.org/abstracts/search?q=degradation" title=" degradation"> degradation</a>, <a href="https://publications.waset.org/abstracts/search?q=plant-bacteria%20partnerships" title=" plant-bacteria partnerships"> plant-bacteria partnerships</a> </p> <a href="https://publications.waset.org/abstracts/104417/enhanced-degradation-of-endosulfan-in-soil-using-lycopersicon-esculentum-l-tomato-and-endosulfan-tolerant-bacterium-strains" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104417.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">151</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">22</span> Stability of a Biofilm Reactor Able to Degrade a Mixture of the Organochlorine Herbicides Atrazine, Simazine, Diuron and 2,4-Dichlorophenoxyacetic Acid to Changes in the Composition of the Supply Medium</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=I.%20Nava-Arenas">I. Nava-Arenas</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Ruiz-Ordaz"> N. Ruiz-Ordaz</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20J.%20Galindez-Mayer"> C. J. Galindez-Mayer</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20L.%20Luna-Guido"> M. L. Luna-Guido</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20L.%20Ruiz-L%C3%B3pez"> S. L. Ruiz-López</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Cabrera-Orozco"> A. Cabrera-Orozco</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Nava-Arenas"> D. Nava-Arenas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Among the most important herbicides, the organochlorine compounds are of considerable interest due to their recalcitrance to the chemical, biological, and photolytic degradation, their persistence in the environment, their mobility, and their bioacummulation. The most widely used herbicides in North America are primarily 2,4-dichlorophenoxyacetic acid (2,4-D), the triazines (atrazine and simazine), and to a lesser extent diuron. The contamination of soils and water bodies frequently occurs by mixtures of these xenobiotics. For this reason, in this work, the operational stability to changes in the composition of the medium supplied to an aerobic biofilm reactor was studied. The reactor was packed with fragments of volcanic rock that retained a complex microbial film, able to degrade a mixture of organochlorine herbicides atrazine, simazine, diuron and 2,4-D, and whose members have microbial genes encoding the main catabolic enzymes atzABCD, tfdACD and puhB. To acclimate the attached microbial community, the biofilm reactor was fed continuously with a mineral minimal medium containing the herbicides (in mg•L-1): diuron, 20.4; atrazine, 14.2, simazine, 11.4, and 2,4-D, 59.7, as carbon and nitrogen sources. Throughout the bioprocess, removal efficiencies of 92-100% for herbicides, 78-90% for COD, 92-96% for TOC and 61-83% for dehalogenation were reached. In the microbial community, the genes encoding catabolic enzymes of different herbicides tfdACD, puhB and, occasionally, the genes atzA and atzC were detected. After the acclimatization, the triazine herbicides were eliminated from the mixture formulation. Volumetric loading rates of the mixture 2,4-D and diuron were continuously supplied to the reactor (1.9-21.5 mg herbicides •L-1 •h-1). Along the bioprocess, the removal efficiencies obtained were 86-100% for the mixture of herbicides, 63-94% for for COD, 90-100% for COT, and dehalogenation values of 63-100%. It was also observed that the genes encoding the enzymes in the catabolism of both herbicides, tfdACD and puhB, were consistently detected; and, occasionally, the atzA and atzC. Subsequently, the triazine herbicide atrazine and simazine were restored to the medium supply. Different volumetric charges of this mixture were continuously fed to the reactor (2.9 to 12.6 mg herbicides •L-1 •h-1). During this new treatment process, removal efficiencies of 65-95% for the mixture of herbicides, 63-92% for COD, 66-89% for TOC and 73-94% of dehalogenation were observed. In this last case, the genes tfdACD, puhB and atzABC encoding for the enzymes involved in the catabolism of the distinct herbicides were consistently detected. The atzD gene, encoding the cyanuric hydrolase enzyme, could not be detected, though it was determined that there was partial degradation of cyanuric acid. In general, the community in the biofilm reactor showed some catabolic stability, adapting to changes in loading rates and composition of the mixture of herbicides, and preserving their ability to degrade the four herbicides tested; although, there was a significant delay in the response time to recover to degradation of the herbicides. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biodegradation" title="biodegradation">biodegradation</a>, <a href="https://publications.waset.org/abstracts/search?q=biofilm%20reactor" title=" biofilm reactor"> biofilm reactor</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20community" title=" microbial community"> microbial community</a>, <a href="https://publications.waset.org/abstracts/search?q=organochlorine%20herbicides" title=" organochlorine herbicides"> organochlorine herbicides</a> </p> <a href="https://publications.waset.org/abstracts/7490/stability-of-a-biofilm-reactor-able-to-degrade-a-mixture-of-the-organochlorine-herbicides-atrazine-simazine-diuron-and-24-dichlorophenoxyacetic-acid-to-changes-in-the-composition-of-the-supply-medium" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/7490.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">435</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">21</span> Reduction of Chlordecone Rates in Bioelectrochemicals Systems from Water and Sediment Swamp Mangrove in Absence of a Redox Mediator</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Malory%20Beaujolais">Malory Beaujolais</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Chlordecone is an organochlorine pesticide with a bishomocubane structure which led to high stability in organic matter. Microbial fuel cell is a type of electrochemical system that can convert organic matters into electricity thanks to electroactive bacteria. This technique has been used with mangrove swamp from Martinique to try to reduce chlordecone rates. Those experiments led to characterize the behavior of the electroactive biofilm formed at the cathode, without added redox mediator. The designed bioelectrochemical system seems to provide the necessary conditions for chlordecone degradation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioelectrochemistry" title="bioelectrochemistry">bioelectrochemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=bioremediation" title=" bioremediation"> bioremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=chlordecone" title=" chlordecone"> chlordecone</a>, <a href="https://publications.waset.org/abstracts/search?q=mangrove%20swamp" title=" mangrove swamp"> mangrove swamp</a> </p> <a href="https://publications.waset.org/abstracts/186572/reduction-of-chlordecone-rates-in-bioelectrochemicals-systems-from-water-and-sediment-swamp-mangrove-in-absence-of-a-redox-mediator" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186572.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">40</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">20</span> Multi-Residue Analysis (GC-ECD) of Some Organochlorine Pesticides in Commercial Broiler Meat Marketed in Shivamogga City, Karnataka State, India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=L.%20V.%20Lokesha">L. V. Lokesha</a>, <a href="https://publications.waset.org/abstracts/search?q=Jagadeesh%20S.%20Sanganal"> Jagadeesh S. Sanganal</a>, <a href="https://publications.waset.org/abstracts/search?q=Yogesh%20S.%20Gowda"> Yogesh S. Gowda</a>, <a href="https://publications.waset.org/abstracts/search?q=Shekhar"> Shekhar</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20B.%20Shridhar"> N. B. Shridhar</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Prakash"> N. Prakash</a>, <a href="https://publications.waset.org/abstracts/search?q=Prashantkumar%20Waghe"> Prashantkumar Waghe</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20D.%20Narayanaswamy"> H. D. Narayanaswamy</a>, <a href="https://publications.waset.org/abstracts/search?q=Girish%20V.%20Kumar"> Girish V. Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Organochlorine (OC) insecticides are among the most important organotoxins and make a large group of pesticides. Physicochemical properties of these toxins, especially their lipophilicity, facilitate the absorption and storage of these toxins in the meat thus possess public health threat to humans. The presence of these toxins in broiler meat can be a quantitative and qualitative index for the presence of these toxins in animal bodies, which is attributed to Waste water of irrigation after spraying the crops, contaminated animal feeds with pesticides, polluted air are the potential sources of residues in animal products. Fifty broiler meat samples were collected from different retail outlets of Bengaluru city, Karnataka state, in ice cold conditions and later stored under -20°C until analysis. All the samples were subjected to Gas Chromatograph attached to Electron Capture Detector(GC-ECD, VARIAN make) screening and quantification of OC pesticides viz; Alachlor, Aldrin, Alpha-BHC, Beta-BHC, Dieldrin, Delta-BHC, o,p-DDE, p,p-DDE, o,p-DDD, p,p-DDD, o,p-DDT, p,p-DDT, Endosulfan-I, Endosulfan-II, Endosulfan Sulphate and Lindane(all the standards were procured from Merck). Extraction was undertaken by blending fifty grams (g) of meat sample with 50g Sodium Sulphate anahydrous, 120 ml of n-hexane, 120 ml acetone for 15 mins, extract is washed with distilled water and sample moisture is dried by sodium sulphate anahydrous, partitioning is done with 25 ml petroleum ether, 10 ml acetonitrile and 15 ml n-hexane shake vigorously for two minutes, sample clean up was done with florosil column. The reconstituted samples (using n-hexane) (Merck chem) were injected to Gas Chromatograph–Electron Capture Detector(GC-ECD). The present study reveals that, among the fifty chicken samples subjected for analysis, 60% (15/50), 32% (8/50), 28% (7/50), 20% (5/50) and 16% (4/50) of samples contaminated with DDTs, Delta-BHC, Dieldrin, Aldrin and Alachlor respectively. DDT metabolites, Delta-BHC were the most frequently detected OC pesticides. The detected levels of the pesticides were below the levels of MRL(according to Export Council of India notification for fresh poultry meat). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=accuracy" title="accuracy">accuracy</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography" title=" gas chromatography"> gas chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=meat" title=" meat"> meat</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticide" title=" pesticide"> pesticide</a>, <a href="https://publications.waset.org/abstracts/search?q=petroleum%20ether" title=" petroleum ether"> petroleum ether</a> </p> <a href="https://publications.waset.org/abstracts/64577/multi-residue-analysis-gc-ecd-of-some-organochlorine-pesticides-in-commercial-broiler-meat-marketed-in-shivamogga-city-karnataka-state-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/64577.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">327</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">19</span> Degradation of Endosulfan in Different Soils by Indigenous and Adapted Microorganisms</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20%C3%96zyer">A. Özyer</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20G.%20Turan"> N. G. Turan</a>, <a href="https://publications.waset.org/abstracts/search?q=Y.%20Ardal%C4%B1"> Y. Ardalı</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The environmental fate of organic contaminants in soils is influenced significantly by the pH, texture of soil, water content and also presence of organic matter. In this study, biodegradation of endosulfan isomers was studied in two different soils (Soil A and Soil B) that have contrasting properties in terms of their texture, pH, organic content, etc. Two <em>Nocardia </em>sp., which were isolated from soil, were used for degradation of endosulfan. Soils were contaminated with commercial endosulfan. Six sets were maintained from two different soils, contaminated with different endosulfan concentrations for degradation experiments. Inoculated and uninoculated mineral media with <em>Nocardia</em> isolates were added to the soils and mixed. Soils were incubated at a certain temperature (30 &deg;C) during ten weeks. Residue endosulfan and its metabolites&rsquo; concentrations were determined weekly during the incubation period. The changes of the soil microorganisms were investigated weekly. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endosulfan" title="endosulfan">endosulfan</a>, <a href="https://publications.waset.org/abstracts/search?q=biodegradation" title=" biodegradation"> biodegradation</a>, <a href="https://publications.waset.org/abstracts/search?q=Nocardia%20sp.%20soil" title=" Nocardia sp. soil"> Nocardia sp. soil</a>, <a href="https://publications.waset.org/abstracts/search?q=organochlorine%20pesticide" title=" organochlorine pesticide"> organochlorine pesticide</a> </p> <a href="https://publications.waset.org/abstracts/48178/degradation-of-endosulfan-in-different-soils-by-indigenous-and-adapted-microorganisms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/48178.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">382</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">18</span> Occurrence and Spatial Distribution of Pesticide Residues in Butter and Ghee (Clarified Butter Fat) in Punjab (India)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=J.%20S.%20Bedi">J. S. Bedi</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20P.%20S.%20Gill"> J. P. S. Gill</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20S.%20Aulakh"> R. S. Aulakh</a>, <a href="https://publications.waset.org/abstracts/search?q=Prabhjit%20Kaur"> Prabhjit Kaur </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present study was undertaken to monitor organochlorine, organophosphate and synthetic pyrethroid pesticide residues in butter and ghee samples collected from six different districts of Punjab. The estimation of pesticide residues was done by multiple residue analytical technique using gas chromatography equipped with GC-ECD and GC-FTD. The confirmation of residues was done on gas chromatography mass spectrometry in both SIM and Scan mode. Results indicated the presence of HCH and pp DDE as predominant contaminant in both butter and ghee even after their ban/restriction on usage in India. Residues of HCH were detected in 25.5 and 23.2 % samples of butter and ghee, respectively, while residues of pp DDE were recorded in 29.3 and 25.0 % butter and ghee samples, respectively. More importantly, the presence of endosulfan, cypermethrin, fenvalerate, deltamethrin and chlorpyrifos was observed in few butter and ghee samples indicating the serious concerns. The spatial variation of pesticide residues occurrence indicated the cotton belt of Punjab as most affected. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=butter" title="butter">butter</a>, <a href="https://publications.waset.org/abstracts/search?q=ghee" title=" ghee"> ghee</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticides%20residues" title=" pesticides residues"> pesticides residues</a>, <a href="https://publications.waset.org/abstracts/search?q=Punjab" title=" Punjab"> Punjab</a> </p> <a href="https://publications.waset.org/abstracts/24490/occurrence-and-spatial-distribution-of-pesticide-residues-in-butter-and-ghee-clarified-butter-fat-in-punjab-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24490.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">429</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">17</span> Estimation of Level of Pesticide in Recurrent Pregnancy Loss and Its Correlation with Paraoxanase1 Gene in North Indian Population</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Apurva%20Singh">Apurva Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20P.%20Jaiswar"> S. P. Jaiswar</a>, <a href="https://publications.waset.org/abstracts/search?q=Apala%20Priyadarshini"> Apala Priyadarshini</a>, <a href="https://publications.waset.org/abstracts/search?q=Akancha%20Pandey"> Akancha Pandey</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: The aim of this study is to find the association of PON1 gene polymorphism with pesticides In RPL subjects. Background: Recurrent pregnancy loss (RPL) is defined as three or more sequential abortions before the 20th week of gestation. Pesticides and its derivatives (organochlorine and organophosphate) are proposed to accommodate a ruler chemical for RPL in the sub-humid region of India. The paraoxonase-1 enzyme (PON1) plays an important role in the toxicity of some organophosphate pesticides, with low PON1 activity being associated with higher pesticide sensitivity Methodology: This is a case-control study done in Department of Obstetrics & Gynaecology & Department of Biochemistry, K.G.M.U, Lucknow, India. The subjects were enrolled after fulfilling the inclusion & exclusion criteria. Inclusion criteria: Cases- Subject having two or more spontaneous abortions & Control- Healthy female having one or more alive child was selected. Exclusion criteria: Cases & Control- Subject having the following disease will be excluded from the study Diabetes mellitus, Hypertension, Tuberculosis, Immunocompromised patients, any endocrine disorder and genital, colon or breast cancer any other malignancies. Blood samples were collected in EDTA tubes from cases & healthy control women & genomic DNA was extracted by phenol-chloroform method. The estimation of pesticides residue from blood was done by HPLC. Biochemical estimation was also performed. Genotyping of PON1 gene polymorphism was performed by RFLP. Statistical analysis of the data was performed using the SPSS16.3 software. Results: A sum of total 14 pesticides (12 organochlorine and 2 organophosphate) selected on the basis of their persistent nature and consumption rate. The significant level of pesticide (ppb) estimated by the Mann whiney test and it was found to be significant at higher level of β-HCH (p:0.04), γ-HCH (p:0.001), δ-HCH (p: 0.002), chloropyrifos (p:0.001), pp-DDD (p:0.001) and fenvalrate (p: 0.001) in case group compare to its control. The level of antioxidant enzymes were found to be significantly decreased among the cases. Wild homozygous TT was more frequent and prevalent among control groups. However, heterozygous group (Tt) was more in cases than control groups (CI-0.3-1.3) (p=0.06). Conclusion: Higher levels of pesticides with endocrine disrupting potential in cases indicate the possible role of these compounds as one of the causes of recurrent pregnancy loss. Possibly, increased pesticide level appears to indicate increased levels of oxidative damage that has been associated with the possible cause of Recurrent Miscarriage, it may reflect indirect evidence of toxicity rather than the direct cause. Since both factors are reported to increase risk, individuals with higher levels of these 'Toxic compounds' especially in 'high-risk genotypes' might be more susceptible to recurrent pregnancy loss. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=paraoxonase" title="paraoxonase">paraoxonase</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticides" title=" pesticides"> pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=PON1" title=" PON1"> PON1</a>, <a href="https://publications.waset.org/abstracts/search?q=RPL" title=" RPL"> RPL</a> </p> <a href="https://publications.waset.org/abstracts/92157/estimation-of-level-of-pesticide-in-recurrent-pregnancy-loss-and-its-correlation-with-paraoxanase1-gene-in-north-indian-population" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/92157.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">143</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">16</span> Characterization of a Three-Electrodes Bioelectrochemical System from Mangrove Water and Sediments for the Reduction of Chlordecone in Martinique</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Malory%20Jonata">Malory Jonata</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Chlordecone (CLD) is an organochlorine pesticide used between 1971 and 1993 in both Guadeloupe and Martinique for the control of banana black weevil. The bishomocubane structure which characterizes this chemical compound led to high stability in organic matter and high persistence in the environment. Recently, researchers found that CLD can be degraded by isolated bacteria consortiums and, particularly, by bacteria such as Citrobacter sp 86 and Delsulfovibrio sp 86. Actually, six transformation product families of CLD are known. Moreover, the latest discovery showed that CLD was disappearing faster than first predicted in highly contaminated soil in Guadeloupe. However, the toxicity of transformation products is still unknown, and knowledge has to be deepened on the degradation ways and chemical characteristics of chlordecone and its transformation products. Microbial fuel cells (MFC) are electrochemical systems that can convert organic matter into electricity thanks to electroactive bacteria. These bacteria can exchange electrons through their membranes to solid surfaces or molecules. MFC have proven their efficiency as bioremediation systems in water and soils. They are already used for the bioremediation of several organochlorine compounds such as perchlorate, trichlorophenol or hexachlorobenzene. In this study, a three-electrodes system, inspired by MFC, is used to try to degrade chlordecone using bacteria from a mangrove swamp in Martinique. As we know, some mangrove bacteria are electroactive. Furthermore, the CLD rate seems to decline in mangrove swamp sediments. This study aims to prove that electroactive bacteria from a mangrove swamp in Martinique can degrade CLD thanks to a three-electrodes bioelectrochemical system. To achieve this goal, the tree-electrodes assembly has been connected to a potentiostat. The substrate used is mangrove water and sediments sampled in the mangrove swamp of La Trinité, a coastal city in Martinique, where CLD contamination has already been studied. Electroactive biofilms are formed by imposing a potential relative to Saturated Calomel Electrode using chronoamperometry. Moreover, their comportment has been studied by using cyclic voltametry. Biofilms have been studied under different imposed potentials, several conditions of the substrate and with or without CLD. In order to quantify the evolution of CLD rates in the substrate’s system, gas chromatography coupled with mass spectrometry (GC-MS) was performed on pre-treated samples of water and sediments after short, medium and long-term contact with the electroactive biofilms. Results showed that between -0,8V and -0,2V, the three-electrodes system was able to reduce the chemical in the substrate solution. The first GC-MS analysis result of samples spiked with CLD seems to reveal decreased CLD concentration over time. In conclusion, the designed bioelectrochemical system can provide the necessary conditions for chlordecone degradation. However, it is necessary to improve three-electrodes control settings in order to increase degradation rates. The biological pathways are yet to enlighten by biologicals analysis of electroactive biofilms formed in this system. Moreover, the electrochemical study of mangrove substrate gives new informations on the potential use of this substrate for bioremediation. But further studies are needed to a better understanding of the electrochemical potential of this environment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioelectrochemistry" title="bioelectrochemistry">bioelectrochemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=bioremediation" title=" bioremediation"> bioremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=chlordecone" title=" chlordecone"> chlordecone</a>, <a href="https://publications.waset.org/abstracts/search?q=mangrove%20swamp" title=" mangrove swamp"> mangrove swamp</a> </p> <a href="https://publications.waset.org/abstracts/165270/characterization-of-a-three-electrodes-bioelectrochemical-system-from-mangrove-water-and-sediments-for-the-reduction-of-chlordecone-in-martinique" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165270.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">83</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">15</span> Monitoring of Pesticide Content in Biscuits Available on the Vojvodina Market, Serbia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ivana%20Loncarevic">Ivana Loncarevic</a>, <a href="https://publications.waset.org/abstracts/search?q=Biljana%20Pajin"> Biljana Pajin</a>, <a href="https://publications.waset.org/abstracts/search?q=Ivana%20Vasiljevic"> Ivana Vasiljevic</a>, <a href="https://publications.waset.org/abstracts/search?q=Milana%20Lazovic"> Milana Lazovic</a>, <a href="https://publications.waset.org/abstracts/search?q=Danica%20Mrkajic"> Danica Mrkajic</a>, <a href="https://publications.waset.org/abstracts/search?q=Aleksandar%20Fises"> Aleksandar Fises</a>, <a href="https://publications.waset.org/abstracts/search?q=Strahinja%20Kovacevic"> Strahinja Kovacevic</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Biscuits belong to a group of flour-confectionery products that are considerably consumed worldwide. The basic raw material for their production is wheat flour or integral flour as a nutritionally highly valuable component. However, this raw material is also a potential source of contamination since it may contain the residues of biochemical compounds originating from plant and soil protection agents. Therefore, it is necessary to examine the health safety of both raw materials and final products. The aim of this research was to examine the content of undesirable residues of pesticides (mostly organochlorine pesticides, organophosphorus pesticides, carbamate pesticides, triazine pesticides, and pyrethroid pesticides) in 30 different biscuit samples of domestic origin present on the Vojvodina market using Gas Chromatograph Thermo ISQ/Trace 1300. The results showed that all tested samples had the limit of detection of pesticide content below 0.01 mg/kg, indicating that this type of confectionary products is not contaminated with pesticides. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biscuits" title="biscuits">biscuits</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticides" title=" pesticides"> pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=contamination" title=" contamination"> contamination</a>, <a href="https://publications.waset.org/abstracts/search?q=quality" title=" quality"> quality</a> </p> <a href="https://publications.waset.org/abstracts/100321/monitoring-of-pesticide-content-in-biscuits-available-on-the-vojvodina-market-serbia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/100321.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">184</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">14</span> Short-Term Exposing Effects of 4,4&#039;-DDT on Mitochondrial Electron Transport Complexes in Eyes of Zebrafish</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eun%20Ko">Eun Ko</a>, <a href="https://publications.waset.org/abstracts/search?q=Moonsung%20Choi"> Moonsung Choi</a>, <a href="https://publications.waset.org/abstracts/search?q=Sooim%20Shin"> Sooim Shin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> 4,4’-Dichlorodiphenyltrichloroethane (4,4’-DDT) is colorless, odorless organochlorine and known as persistent toxic organic pollutant accumulated in organs. In this study, effects of 4,4’-DDT on activities of mitochondrial electron transport chain system was analyzed. 4,4’-DDT is directly treated to isolated mitochondria from eyes of zebrafish and then activities of mitochondrial complex I, II, III, IV were measured spectrophotometrically. The reaction was proceeded immediately after adding 4,4’-DDT to examine the short-term exposing effects of persistent organic pollutant. As a result, high concentration of 4,4’-DDT treated mitochondria exhibited slightly enhanced activity in all complexes than non-treated one except complex III in male. Particularly, 4,4’-DDT was more effective on enzymatic activity in mitochondria isolated from eyes of male zebrafish. These results represented that 4,4’-DDT might temporarily induce to open up ion channel on isolated mitochondria resulting in increasing the functional activity of mitochondrial electron transport chain system. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electron%20transport%20chain" title="electron transport chain">electron transport chain</a>, <a href="https://publications.waset.org/abstracts/search?q=mitochondrial%20function" title=" mitochondrial function"> mitochondrial function</a>, <a href="https://publications.waset.org/abstracts/search?q=persistent%20organic%20pollutant" title=" persistent organic pollutant"> persistent organic pollutant</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometric%20assay" title=" spectrophotometric assay"> spectrophotometric assay</a>, <a href="https://publications.waset.org/abstracts/search?q=zebrafish" title=" zebrafish "> zebrafish </a> </p> <a href="https://publications.waset.org/abstracts/77446/short-term-exposing-effects-of-44-ddt-on-mitochondrial-electron-transport-complexes-in-eyes-of-zebrafish" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77446.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">228</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> A Review on the Mechanism Removal of Pesticides and Heavy Metal from Agricultural Runoff in Treatment Train</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=N.%20A.%20Ahmad%20Zubairi">N. A. Ahmad Zubairi</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Takaijudin"> H. Takaijudin</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20W.%20Yusof"> K. W. Yusof</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Pesticides have been used widely over the world in agriculture to protect from pests and reduce crop losses. However, it affects the environment with toxic chemicals. Exceed of toxic constituents in the ecosystem will result in bad side effects. The hydrological cycle is related to the existence of pesticides and heavy metal which it can penetrate through varieties of sources into the soil or water bodies, especially runoff. Therefore, proper mechanisms of pesticide and heavy metal removal should be studied to improve the quality of ecosystem free or reduce from unwanted substances. This paper reviews the use of treatment train and its mechanisms to minimize pesticides and heavy metal from agricultural runoff. Organochlorine (OCL) is a common pesticide that was found in the agricultural runoff. OCL is one of the toxic chemicals that can disturb the ecosystem such as inhibiting plants&#39; growth and harm human health by having symptoms as asthma, active cancer cell, vomit, diarrhea, etc. Thus, this unwanted contaminant gives disadvantages to the environment and needs treatment system. Hence, treatment train by bioretention system is suitable because removal efficiency achieves until 90% of pesticide removal with selected vegetated plant and additive. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pesticides" title="pesticides">pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=heavy%20metal" title=" heavy metal"> heavy metal</a>, <a href="https://publications.waset.org/abstracts/search?q=agricultural%20runoff" title=" agricultural runoff"> agricultural runoff</a>, <a href="https://publications.waset.org/abstracts/search?q=bioretention" title=" bioretention"> bioretention</a>, <a href="https://publications.waset.org/abstracts/search?q=mechanism%20removal" title=" mechanism removal"> mechanism removal</a>, <a href="https://publications.waset.org/abstracts/search?q=treatment%20train" title=" treatment train"> treatment train</a> </p> <a href="https://publications.waset.org/abstracts/130426/a-review-on-the-mechanism-removal-of-pesticides-and-heavy-metal-from-agricultural-runoff-in-treatment-train" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/130426.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">158</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> Different Tools and Complex Approach for Improving Phytoremediation Technology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=T.%20Varazi">T. Varazi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Pruidze"> M. Pruidze</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Kurashvili"> M. Kurashvili</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Gagelidze"> N. Gagelidze</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Sutton"> M. Sutton</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The complex phytoremediation approach given in the presented work implies joint application of natural sorbents, microorganisms, natural biosurfactants and plants. The approach is based on using the natural mineral composites, microorganism strains with high detoxification abilities, plants-phytoremediators and natural biosurfactants for enhancing the uptake of intermediates of pollutants by plant roots. In this complex strategy of phytoremediation technology, the sorbent serves to uptake and trap the pollutants and thus restrain their emission in the environment. The role of microorganisms is to accomplish the first stage biodegradation of organic contaminants. This is followed by application of a phytoremediation technology through purposeful planting of selected plants. Thus, using of different tools will provide restoration of polluted environment and prevention of toxic compounds’ dissemination from hotbeds of pollution for a considerable length of time. The main idea and novelty of the carried out work is the development of a new approach for the ecological safety. The wide spectrum of contaminants: Organochlorine pesticide – DDT, heavy metal –Cu, oil hydrocarbon (hexadecane) and wax have been used in this work. The presented complex biotechnology is important from the viewpoint of prevention, providing total rehabilitation of soil. It is unique to chemical pollutants, ecologically friendly and provides the control of erosion of soils. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioremediation" title="bioremediation">bioremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=phytoremediation" title=" phytoremediation"> phytoremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=pollutants" title=" pollutants"> pollutants</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20contamination" title=" soil contamination"> soil contamination</a> </p> <a href="https://publications.waset.org/abstracts/28936/different-tools-and-complex-approach-for-improving-phytoremediation-technology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28936.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">297</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">11</span> A Comprehensive Review on Health Hazards and Challenges for Microbial Remediation of Persistent Organic Pollutants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nisha%20Gaur">Nisha Gaur</a>, <a href="https://publications.waset.org/abstracts/search?q=K.Narasimhulu"> K.Narasimhulu</a>, <a href="https://publications.waset.org/abstracts/search?q=Pydi%20Setty%20Yelamarthy"> Pydi Setty Yelamarthy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Persistent organic pollutants (POPs) have become a great concern due to their toxicity, transformation and bioaccumulation property. Therefore, this review highlights the types, sources, classification health hazards and mobility of organochlorine pesticides, industrial chemicals and their by-products. Moreover, with the signing of Aarhus and Stockholm convention on POPs there is an increased demand to identify and characterise such chemicals from industries and environment which are toxic in nature or to existing biota. Due to long life, persistent nature they enter into body through food and transfer to all tropic levels of ecological unit. In addition, POPs are lipophilic in nature and accumulate in lipid-containing tissues and organs which further indicates the adverse symptoms after the threshold limit. Though, several potential enzymes are reported from various categories of microorganism and their interaction with POPs may break down the complex compounds either through biodegradation, biostimulation or bioaugmentation process, however technological advancement and human activities have also indicated to explore the possibilities for the role of genetically modified organisms and metagenomics and metabolomics. Though many studies have been done to develop low cost, effective and reliable method for detection, determination and removal of ultra-trace concentration of persistent organic pollutants (POPs) but due to insufficient knowledge and non-feasibility of technique, the safe management of POPs is still a global challenge. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=persistent%20organic%20pollutants" title="persistent organic pollutants">persistent organic pollutants</a>, <a href="https://publications.waset.org/abstracts/search?q=bioaccumulation" title=" bioaccumulation"> bioaccumulation</a>, <a href="https://publications.waset.org/abstracts/search?q=biostimulation" title=" biostimulation"> biostimulation</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20remediation" title=" microbial remediation"> microbial remediation</a> </p> <a href="https://publications.waset.org/abstracts/76893/a-comprehensive-review-on-health-hazards-and-challenges-for-microbial-remediation-of-persistent-organic-pollutants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/76893.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">299</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10</span> Green Synthesis of Magnetic, Silica Nanocomposite and Its Adsorptive Performance against Organochlorine Pesticides</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Waleed%20A.%20El-Said">Waleed A. El-Said</a>, <a href="https://publications.waset.org/abstracts/search?q=Dina%20M.%20Fouad"> Dina M. Fouad</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20H.%20Aly"> Mohamed H. Aly</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20A.%20El-Gahami"> Mohamed A. El-Gahami</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Green synthesis of nanomaterials has received increasing attention as an eco-friendly technology in materials science. Here, we have used two types of extractions from green tea leaf (i.e. total extraction and tannin extraction) as reducing agents for a rapid, simple and one step synthesis method of mesoporous silica nanoparticles (MSNPs)/iron oxide (Fe3O4) nanocomposite based on deposition of Fe3O4 onto MSNPs. MSNPs/Fe3O4 nanocomposite were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray, vibrating sample magnetometer, N2 adsorption, and high-resolution transmission electron microscopy. The average mesoporous silica particle diameter was found to be around 30 nm with high surface area (818 m2/gm). MSNPs/Fe3O4 nanocomposite was used for removing lindane pesticide (an environmental hazard material) from aqueous solutions. Fourier transform infrared, UV-vis, High-performance liquid chromatography and gas chromatography techniques were used to confirm the high ability of MSNPs/Fe3O4 nanocomposite for sensing and capture of lindane molecules with high sorption capacity (more than 89%) that could develop a new eco-friendly strategy for detection and removing of pesticide and as a promising material for water treatment application. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=green%20synthesis" title="green synthesis">green synthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=mesoporous%20silica" title=" mesoporous silica"> mesoporous silica</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetic%20iron%20oxide%20NPs" title=" magnetic iron oxide NPs"> magnetic iron oxide NPs</a>, <a href="https://publications.waset.org/abstracts/search?q=adsorption%20Lindane" title=" adsorption Lindane"> adsorption Lindane</a> </p> <a href="https://publications.waset.org/abstracts/74140/green-synthesis-of-magnetic-silica-nanocomposite-and-its-adsorptive-performance-against-organochlorine-pesticides" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74140.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">436</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Effect of Organochlorine Insecticide (Endosulfan) on Albino Rat at the Rate of Blood Uric Acid Level</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bindu%20Kumari">Bindu Kumari</a>, <a href="https://publications.waset.org/abstracts/search?q=Bindu%20Kumari%20Singh"> Bindu Kumari Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Endosulfan is known to be one of the highly toxic agricultural pesticides commonly used in our societies. With the widespread use of Endosulfan in agriculture, human beings are most likely to be exposed to it, either orally by eating Endosulfan-contaminated foods or by nose and whole body inhalation in the farms during its application. The present study was conducted to observe the changes in the serum uric acid level of the Swiss albino rats due to the administration of Endosulfan. 3.0 mg Endosulfan/kg body weight was daily administered orally to albino rats for 28 days period. Alterations in their K.F.T. parameters were recorded at a regular interval of 7 days within this 28 days period and were compared with those of control rats. All rats were monitored for any observable toxic symptoms throughout the experimental period and they also were weighted weekly to monitor body weight gain. Alteration recorded in K.F.T. parameters within the groups were due to Endosulfan exposure and serum uric acid level was significantly elevated in the 3mg/kg dose group. Pathological changes of rats treated with Endosulfan were observed with typical signs of toxicity. Uric acid is a heterocyclic compound formed as an end product of metabolism of purine nucleotides. It forms ions and salts known as urate and acid urate which are harmful to our health. Uric acid clearance is one of the numerous important functions of the kidney. Defects in this process resulted in Gout, kidney stone or Kidney failure. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=KFT%20parameters" title="KFT parameters">KFT parameters</a>, <a href="https://publications.waset.org/abstracts/search?q=blood%20uric%20acid%20level" title=" blood uric acid level"> blood uric acid level</a>, <a href="https://publications.waset.org/abstracts/search?q=endosulfan" title=" endosulfan"> endosulfan</a>, <a href="https://publications.waset.org/abstracts/search?q=eat" title=" eat"> eat</a> </p> <a href="https://publications.waset.org/abstracts/29538/effect-of-organochlorine-insecticide-endosulfan-on-albino-rat-at-the-rate-of-blood-uric-acid-level" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29538.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">291</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Toxic Influence of Cypermethrin on Biochemical Changes in Fresh Water Fish, Cyprinus carpio</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gowri%20Balaji">Gowri Balaji</a>, <a href="https://publications.waset.org/abstracts/search?q=Muthusamy%20Nachiyappan"> Muthusamy Nachiyappan</a>, <a href="https://publications.waset.org/abstracts/search?q=Ramalingam%20Venugopal"> Ramalingam Venugopal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Amongst the wide spectrum of pesticides, pyrethroids are preferably used rather than organochlorine, organophosphorous and carbamates pesticides due to their high effectiveness. Synthetic pyrethroids which are the chemicals used for the pest control in agriculture are now being excessively used in India. The aim of the present study was to evaluate the adverse effect of cypermethrin on the fresh water fish Cyprinus carpio, the common carp. The effect was assessed by comparing the biochemical parameters in the blood and liver tissues of control fishes with three experimental group of fishes exposed with cypermethrin for 7 days 1/15 Lc50 (E1) 1/10 Lc50 (E2) and 1/5 Lc50 values (E3). After 7 days of exposure, blood was collected and liver and gills was dissected out. The activities of acid phosphatase, alkaline phosphatase, lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase were estimated by standard spectrophotometric techniques in the blood, liver and gills tissue homogenate. Lactate dehydrogenase was significantly decreased in E2 and E3 experimental groups. The activities of acid phosphatase, alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase were significantly altered in the experimental groups. All the biochemical parameters studied were adversely affected in the liver and gills of cypermethrin exposed fish. The results obtained from the present study of cypermethrin exposed fishes indicate a marked toxic effect of cypermethrin and also its dose dependent impact on different organs of the fish. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cypermethrin" title="cypermethrin">cypermethrin</a>, <a href="https://publications.waset.org/abstracts/search?q=Cyprinus%20carpio" title=" Cyprinus carpio"> Cyprinus carpio</a>, <a href="https://publications.waset.org/abstracts/search?q=ALT" title=" ALT"> ALT</a>, <a href="https://publications.waset.org/abstracts/search?q=AST" title=" AST"> AST</a>, <a href="https://publications.waset.org/abstracts/search?q=LDH" title=" LDH"> LDH</a>, <a href="https://publications.waset.org/abstracts/search?q=liver" title=" liver"> liver</a>, <a href="https://publications.waset.org/abstracts/search?q=gills" title=" gills"> gills</a> </p> <a href="https://publications.waset.org/abstracts/10239/toxic-influence-of-cypermethrin-on-biochemical-changes-in-fresh-water-fish-cyprinus-carpio" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10239.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">286</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Functional Role of Tyr12 in the Catalytic Activity of Zeta-Like Glutathione S-Transferase from Acidovorax sp. KKS102</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=D.%20Shehu">D. Shehu</a>, <a href="https://publications.waset.org/abstracts/search?q=Z.%20Alias"> Z. Alias </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Glutathione S-transferases (GSTs) are family of enzymes that function in the detoxification of variety of electrophilic substrates. In the present work, we report a novel zeta-like GST (designated as KKSG9) from the biphenyl/polychlorobiphenyl degrading organism Acidovorax sp. KKS102. KKSG9 possessed low sequence similarity but similar biochemical properties to zeta class GSTs. The gene for KKSG9 was cloned, purified and biochemically characterized. Functional analysis showed that the enzyme exhibits wider substrate specificity compared to most zeta class GSTs by reacting with 1-chloro-2,4-dinitrobenzene (CDNB), p-nitrobenzyl chloride (NBC), ethacrynic acid (EA), hydrogen peroxide, and cumene hydroperoxide (CuOOH). The enzyme also displayed dehalogenation function against dichloroacetate (a common substrate for zeta class GSTs) in addition to permethrin, and dieldrin. The functional role of Tyr12 was also investigated by site-directed mutagenesis. The mutant (Y12C) displayed low catalytic activity and dehalogenation function against all the substrates when compared with the wild type. Kinetic analysis using NBC and GSH as substrates showed that the mutant (Y12C) displayed a higher affinity for NBC when compared with the wild type, however, no significant change in GSH affinity was observed. These findings suggest that the presence of tyrosine residue in the motif might represent an evolutionary trend toward improving the catalytic activity of the enzyme. The enzyme as well could be useful in the bioremediation of various types of organochlorine pollutants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acidovorax%20sp.%20KKS102" title="Acidovorax sp. KKS102">Acidovorax sp. KKS102</a>, <a href="https://publications.waset.org/abstracts/search?q=bioremediation" title=" bioremediation"> bioremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=glutathione%20s-transferase" title=" glutathione s-transferase"> glutathione s-transferase</a>, <a href="https://publications.waset.org/abstracts/search?q=site-directed%20mutagenesis" title=" site-directed mutagenesis"> site-directed mutagenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=zeta" title=" zeta"> zeta</a> </p> <a href="https://publications.waset.org/abstracts/95033/functional-role-of-tyr12-in-the-catalytic-activity-of-zeta-like-glutathione-s-transferase-from-acidovorax-sp-kks102" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/95033.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">150</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Effect of Botanical and Synthetic Insecticide on Different Insect Pests and Yield of Pea (Pisum sativum)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Saeed">Muhammad Saeed</a>, <a href="https://publications.waset.org/abstracts/search?q=Nazeer%20Ahmed"> Nazeer Ahmed</a>, <a href="https://publications.waset.org/abstracts/search?q=Mukhtar%20Alam"> Mukhtar Alam</a>, <a href="https://publications.waset.org/abstracts/search?q=Fazli%20Subhan"> Fazli Subhan</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Adnan"> Muhammad Adnan</a>, <a href="https://publications.waset.org/abstracts/search?q=Fazli%20Wahid"> Fazli Wahid</a>, <a href="https://publications.waset.org/abstracts/search?q=Hidayat%20Ullah"> Hidayat Ullah</a>, <a href="https://publications.waset.org/abstracts/search?q=Rafiullah"> Rafiullah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present experiment evaluated different synthetic insecticides against Jassid (Amrasca devastations) on pea crop at Agriculture Research Institute Tarnab, Peshawar Khyber Pakhtunkhwa. The field was prepared to cultivate okra crop in Randomized Complete Block (RCB) Design having six treatments with four replications. Plant to plant and row to row distance was kept at 15 cm and 30 cm, respectively. Pre and post spray data were recorded randomly from the top, middle and bottom leaves of five selected plants. Five synthetic insecticides, namely Confidor (Proponil), a neonicotinoid insecticide, Chlorpyrifos (chlorinated organophosphate (OP) insecticide), Lazer (dinitroaniline) (Pendimethaline), Imidacloprid (neonicotinoids insecticide) and Thiodan (Endosulfan, organochlorine insecticide), were used against infestation of aphids, pea pod borer, stem fly, leaf minor and pea weevil. Each synthetic insecticide showed significantly more effectiveness than control (untreated plots) but was non-significant among each other. The lowest population density was recorded in the plot treated with synthetic insecticide i.e. Confidor (0.6175 liter.ha-1) (4.24 aphids plant⁻¹) which is followed by Imidacloprid (0.6175 liter.ha⁻¹) (4.64 pea pod borer plant⁻¹), Thiodan (1.729 liter.ha⁻¹) (4.78 leaf minor plant⁻¹), Lazer (2.47 liter.ha-1) (4.91 pea weevil plant⁻¹), Chlorpyrifos (1.86 liter.ha⁻¹) (5.11 stem fly plant⁻¹), respectively while the highest population was recorded from the control plot. It is concluded from the data that the residual effect decreases with time after the application of spray, which may be less dangerous to the environment and human beings and can effectively manage this dread. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=okra%20crop" title="okra crop">okra crop</a>, <a href="https://publications.waset.org/abstracts/search?q=jassids" title=" jassids"> jassids</a>, <a href="https://publications.waset.org/abstracts/search?q=Confidor" title=" Confidor"> Confidor</a>, <a href="https://publications.waset.org/abstracts/search?q=imidacloprid" title=" imidacloprid"> imidacloprid</a>, <a href="https://publications.waset.org/abstracts/search?q=chlorpyrifos" title=" chlorpyrifos"> chlorpyrifos</a>, <a href="https://publications.waset.org/abstracts/search?q=laser" title=" laser"> laser</a>, <a href="https://publications.waset.org/abstracts/search?q=Thiodan" title=" Thiodan"> Thiodan</a> </p> <a href="https://publications.waset.org/abstracts/147696/effect-of-botanical-and-synthetic-insecticide-on-different-insect-pests-and-yield-of-pea-pisum-sativum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/147696.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">84</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Transcriptomic Response of Calmodulin Encoding Gene (CaM) in Pesticide Utilizing Talaromyces Fungal Strains </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20D.%20Asemoloye">M. D. Asemoloye</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20G.%20Jonathan"> S. G. Jonathan</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Rafiq"> A. Rafiq</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20J.%20Olawuyi"> O. J. Olawuyi</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20O.%20Adejoye"> D. O. Adejoye</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Calmodulin is one of the intracellular calcium proteins that regulates large spectrum of enzymes and cellular functions including metabolism of cyclic nucleotides and glycogen. The potentials of calmodulin gene in fungi necessitates their genetic response and their strong cassette of enzyme secretions for pesticide degradation. Therefore, this study was carried out to investigate the ‘Transcriptomic’ response of calmodulin encoding genes in Talaromyces fungi in response to 2, 2-dichlorovinyl dimethyl phosphate (DDVP or Dichlorvos) an organophosphate pesticide and γ-Hexachlorocyclohexane (Lindane) an organochlorine pesticide. Fungi strains isolated from rhizosphere from grasses rhizosphere in pesticide polluted sites were subjected to percentage incidence test. Two most frequent fungi were further characterized using ITS gene amplification (ITS1 and ITS4 combinations), they were thereafter subjected to In-vitro DDVP and lindane tolerance tests at different concentrations. They were also screened for presence and expression of calmodulin gene (caM) using RT-PCR technique. The two Talaromyces strains had the highest incidence of 50-72% in pesticide polluted site, they were both identified as Talaromyces astroroseus asemoG and Talaromyces purpurogenum asemoN submitted in NCBI gene-bank with accession numbers KY488464 and KY488468 respectively. T. astroroseus KY488464 tolerated DDVP (1.23±0.023 cm) and lindane (1.11±0.018 cm) at 25 % concentration while T. purpurogenum KY488468 tolerated DDVP (1.33±0.061 cm) and lindane (1.54±0.077 cm) at this concentration. Calmodulin gene was detected in both strains, but RT-PCR expression of caM gene revealed at 900-1000 bp showed an under-expression of caM in T. astrorosues KY488464 but overexpressed in T. purpurogenum KY488464. Thus, the calmodulin gene response of these fungal strains to both pesticides could be considered in monitoring the potentials of fungal strains to pesticide tolerance and bioremediation of pesticide in polluted soil. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Calmodulin%20gene" title="Calmodulin gene">Calmodulin gene</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticide" title=" pesticide"> pesticide</a>, <a href="https://publications.waset.org/abstracts/search?q=RT-PCR" title=" RT-PCR"> RT-PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=talaromyces" title=" talaromyces"> talaromyces</a>, <a href="https://publications.waset.org/abstracts/search?q=tolerance" title=" tolerance"> tolerance</a> </p> <a href="https://publications.waset.org/abstracts/77765/transcriptomic-response-of-calmodulin-encoding-gene-cam-in-pesticide-utilizing-talaromyces-fungal-strains" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77765.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">225</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Bacterial Diversity Reports Contamination around the Ichkeul Lake in Tunisia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zeina%20Bourhane">Zeina Bourhane</a>, <a href="https://publications.waset.org/abstracts/search?q=Anders%20Lanzen"> Anders Lanzen</a>, <a href="https://publications.waset.org/abstracts/search?q=Christine%20Cagnon"> Christine Cagnon</a>, <a href="https://publications.waset.org/abstracts/search?q=Olfa%20Ben%20Said"> Olfa Ben Said</a>, <a href="https://publications.waset.org/abstracts/search?q=Cristiana%20Cravo-Laureau"> Cristiana Cravo-Laureau</a>, <a href="https://publications.waset.org/abstracts/search?q=Robert%20Duran"> Robert Duran</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The anthropogenic pressure in coastal areas increases dramatically with the exploitation of environmental resources. Biomonitoring coastal areas are crucial to determine the impact of pollutants on bacterial communities in soils and sediments since they provide important ecosystem services. However, relevant biomonitoring tools allowing fast determination of the ecological status are yet to be defined. Microbial ecology approaches provide useful information for developing such microbial monitoring tools reporting on the effect of environmental stressors. Chemical and microbial molecular approaches were combined in order to determine microbial bioindicators for assessing the ecological status of soil and river ecosystems around the Ichkeul Lake (Tunisia), an area highly impacted by human activities. Samples were collected along soil/river/lake continuums in three stations around the Ichkeul Lake influenced by different human activities at two seasons (summer and winter). Contaminant pressure indexes (PI), including PAHs (Polycyclic aromatic hydrocarbons), alkanes, and OCPs (Organochlorine pesticides) contents, showed significant differences in the contamination level between the stations with seasonal variation. Bacterial communities were characterized by 16S ribosomal RNAs (rRNA) gene metabarcoding. Although microgAMBI indexes, determined from the sequencing data, were in accordance with contaminant contents, they were not sufficient to fully explain the PI. Therefore, further microbial indicators are still to be defined. The comparison of bacterial communities revealed the specific microbial assemblage for soil, river, and lake sediments, which were significantly correlated with contaminant contents and PI. Such observation offers the possibility to define a relevant set of bioindicators for reporting the effects of human activities on the microbial community structure. Such bioindicators might constitute useful monitoring tools for the management of microbial communities in coastal areas. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacterial%20communities" title="bacterial communities">bacterial communities</a>, <a href="https://publications.waset.org/abstracts/search?q=biomonitoring" title=" biomonitoring"> biomonitoring</a>, <a href="https://publications.waset.org/abstracts/search?q=contamination" title=" contamination"> contamination</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20impacts" title=" human impacts"> human impacts</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20bioindicators" title=" microbial bioindicators"> microbial bioindicators</a> </p> <a href="https://publications.waset.org/abstracts/128638/bacterial-diversity-reports-contamination-around-the-ichkeul-lake-in-tunisia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/128638.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">164</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Determination of Pesticides Residues in Tissue of Two Freshwater Fish Species by Modified QuEChERS Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Iwona%20Cie%C5%9Blik">Iwona Cieślik</a>, <a href="https://publications.waset.org/abstracts/search?q=W%C5%82adys%C5%82aw%20Migda%C5%82"> Władysław Migdał</a>, <a href="https://publications.waset.org/abstracts/search?q=Kinga%20Topolska"> Kinga Topolska</a>, <a href="https://publications.waset.org/abstracts/search?q=Ewa%20Cie%C5%9Blik"> Ewa Cieślik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The consumption of fish is recommended as a means of preventing serious diseases, especially cardiovascular problems. Fish is known to be a valuable source of protein (rich in essential amino acids), unsaturated fatty acids, fat-soluble vitamins, macro- and microelements. However, it can also contain several contaminants (e.g. pesticides, heavy metals) that may pose considerable risks for humans. Among others, pesticide are of special concern. Their widespread use has resulted in the contamination of environmental compartments, including water. The occurrence of pesticides in the environment is a serious problem, due to their potential toxicity. Therefore, a systematic monitoring is needed. The aim of the study was to determine the organochlorine and organophosphate pesticide residues in fish muscle tissues of the pike (Esox lucius, L.) and the rainbow trout (Oncorhynchus mykkis, Walbaum) by a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method, using Gas Chromatography Quadrupole Mass Spectrometry (GC/Q-MS), working in selected-ion monitoring (SIM) mode. The analysis of α-HCH, β-HCH, lindane, diazinon, disulfoton, δ-HCH, methyl parathion, heptachlor, malathion, aldrin, parathion, heptachlor epoxide, γ-chlordane, endosulfan, α-chlordane, o,p'-DDE, dieldrin, endrin, 4,4'-DDD, ethion, endrin aldehyde, endosulfan sulfate, 4,4'-DDT, and metoxychlor was performed in the samples collected in the Carp Valley (Malopolska region, Poland). The age of the pike (n=6) was 3 years and its weight was 2-3 kg, while the age of the rainbow trout (n=6) was 0.5 year and its weight was 0.5-1.0 kg. Detectable pesticide (HCH isomers, endosulfan isomers, DDT and its metabolites as well as metoxychlor) residues were present in fish samples. However, all these compounds were below the limit of quantification (LOQ). The other examined pesticide residues were below the limit of detection (LOD). Therefore, the levels of contamination were - in all cases - below the default Maximum Residue Levels (MRLs), established by Regulation (EC) No 396/2005 of the European Parliament and of the Council. The monitoring of pesticide residues content in fish is required to minimize potential adverse effects on the environment and human exposure to these contaminants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=contaminants" title="contaminants">contaminants</a>, <a href="https://publications.waset.org/abstracts/search?q=fish" title=" fish"> fish</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticides%20residues" title=" pesticides residues"> pesticides residues</a>, <a href="https://publications.waset.org/abstracts/search?q=QuEChERS%20method" title=" QuEChERS method"> QuEChERS method</a> </p> <a href="https://publications.waset.org/abstracts/46809/determination-of-pesticides-residues-in-tissue-of-two-freshwater-fish-species-by-modified-quechers-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46809.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">220</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Micro Plasma an Emerging Technology to Eradicate Pesticides from Food Surface</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Saiful%20Islam%20Khan">Muhammad Saiful Islam Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Yun%20Ji%20Kim"> Yun Ji Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Organophosphorus pesticides (OPPs) have been widely used to replace more persistent organochlorine pesticides because OPPs are more soluble in water and decompose rapidly in aquatic systems. Extensive uses of OPPs in modern agriculture are the major cause of the contamination of surface water. Regardless of the advantages gained by the application of pesticides in modern agriculture, they are a threat to the public health environment. With the aim of reducing possible health threats, several physical and chemical treatment processes have been studied to eliminate biological and chemical poisons from food stuff. In the present study, a micro-plasma device was used to reduce pesticides from the surface of food stuff. Pesticide free food items chosen in this study were perilla leaf, tomato, broccoli and blueberry. To evaluate the removal efficiency of pesticides, different washing methods were followed such as soaking with water, washing with bubbling water, washing with plasma-treated water and washing with chlorine water. 2 mL of 2000 ppm pesticide samples, namely, diazinone and chlorpyrifos were individuality inoculated on food surface and was air dried for 2 hours before treated with plasma. Plasma treated water was used in two different manners one is plasma treated water with bubbling the other one is aerosolized plasma treated water. The removal efficiency of pesticides from food surface was studied using HPLC. Washing with plasma treated water, aerosolized plasma treated water and chlorine water shows minimum 72% to maximum 87 % reduction for 4 min treatment irrespective to the types of food items and the types of pesticides sample, in case of soaking and bubbling the reduction is 8% to 48%. Washing with plasma treated water, aerosolized plasma treated water and chlorine water shows somewhat similar reduction ability which is significantly higher comparing to the soaking and bubbling washing system. The temperature effect of the washing systems was also evaluated; three different temperatures were set for the experiment, such as 22°C, 10°C and 4°C. Decreasing temperature from 22°C to 10°C shows a higher reduction in the case of washing with plasma and aerosolized plasma treated water, whereas an opposite trend was observed for the washing with chlorine water. Further temperature reduction from 10°C to 4°C does not show any significant reduction of pesticides, except for the washing with chlorine water. Chlorine water treatment shows lesser pesticide reduction with the decrease in temperature. The color changes of the treated sample were measured immediately and after one week to evaluate if there is any effect of washing with plasma treated water and with chlorine water. No significant color changes were observed for either of the washing systems, except for broccoli washing with chlorine water. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chlorpyrifos" title="chlorpyrifos">chlorpyrifos</a>, <a href="https://publications.waset.org/abstracts/search?q=diazinone" title=" diazinone"> diazinone</a>, <a href="https://publications.waset.org/abstracts/search?q=pesticides" title=" pesticides"> pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=micro%20plasma" title=" micro plasma"> micro plasma</a> </p> <a href="https://publications.waset.org/abstracts/139413/micro-plasma-an-emerging-technology-to-eradicate-pesticides-from-food-surface" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139413.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">187</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=organochlorine&amp;page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=organochlorine&amp;page=2" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">&copy; 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