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<li><a href="https://www.microscopyu.com/galleries/digital-imaging">Digital Imaging</a></li> </ul> </li> <li><a href="/museum">Museum</a></li> </ul> </nav> </div> <section id="searchbox"> <button id="search-button"><img src="/images/search.svg" height="24" width="24"/></button> <p><a href="https://www.microscope.healthcare.nikon.com" class="jumpext">Nikon Instruments</a> | <a target="popup" href="http://www.nikon.com" class="jumpext">Nikon Global</a> | <a href="http://www.nikonsmallworld.com" class="jumpext">Nikon Small World</a></p> </section> </header> <div id="content"> <section id="hpcarousel" class="on1" data-on="1"> <div class="hpcimage image1"> <a href="https://www.microscopyu.com/techniques/super-resolution/single-molecule-super-resolution-imaging"><img src="https://www.microscopyu.com/assets/carousel-images/Homepage-STORM-Masthead.jpg" /></a> </div> <div class="hpcimage image2"> <a 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<nav class="hpcnav"> <ul> <li class="to1" data-to="1">1</li> <li class="to2" data-to="2">2</li> <li class="to3" data-to="3">3</li> </ul> </nav> </section> <div class="leftcontent"> <section class="welcome textblock"> <h2>What's New on MicroscopyU</h2> <p>We鈥檙e happy to announce that we鈥檝e updated our article on Single-Molecule Super-Resolution Imaging. This article now contains an in-depth discussion of sample preparation, including near-comprehensive tables listing known fluorescent probes and buffer systems for single molecule localization microscopies.</p> <p class="learnmore"><a href="https://www.microscopyu.com/techniques/super-resolution/single-molecule-super-resolution-imaging" class="button">Learn More</a></p> </section> <section id="poparticles" class="articlelist"> <h2>Most Viewed Review Articles</h2> <ul> <li class="intro"><h3><a href="https://www.microscopyu.com/microscopy-basics/refractive-index-index-of-refraction">Refractive Index (Index of Refraction)</a></h3> <p>The ratio of the speed of light in a vacuum to that in the imaging medium of a microscope.</p> </li> <li class="intro"><h3><a href="https://www.microscopyu.com/techniques/polarized-light/polarized-light-microscopy">Polarized Light Microscopy</a></h3> <p>Using crossed polarized illumination to examine birefringent materials.</p> </li> <li class="intro"><h3><a href="https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy">Introduction to Phase Contrast Microscopy</a></h3> <p>A mechanism to translate variations in phase into corresponding changes in amplitude.</p> </li> <li class="intro"><h3><a href="https://www.microscopyu.com/techniques/polarized-light/principles-of-birefringence">Principles of Birefringence</a></h3> <p>Defined as double refraction of light in a transparent, molecularly ordered material.</p> </li> </ul> <hr class="clear" /> <p class="viewmore"><a href="/popular-articles" class="button">View More</a></p> </section> <section id="poptuts" class="tutoriallist thumblist"> <h2>Most Viewed Tutorials</h2> <ul> <li> <a href="https://www.microscopyu.com/tutorials/spatial-resolution-in-digital-imaging"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/spatial-resolution.jpg" height="80" width="80" /> <h3>Spatial Resolution in Digital Imaging</h3></a> <p>Spatial resolution refers to the number of pixels utilized in construction of the image.</p> </li> <li> <a href="https://www.microscopyu.com/tutorials/stochastic-optical-reconstruction-microscopy-storm-imaging"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/storm-thumb.jpg" height="80" width="80" /> <h3>Stochastic Optical Reconstruction Microscopy (STORM) Imaging</h3></a> <p>Review the basic steps involved in acquiring a STORM super-resolution image.</p> </li> <li> <a href="https://www.microscopyu.com/tutorials/imageformation-airyna"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/numerical-aperture.jpg" height="80" width="80" /> <h3>Numerical Aperture and Image Resolution</h3></a> <p>Explore how objective numerical aperture size influences Airy disk properties. </p> </li> <li> <a href="https://www.microscopyu.com/tutorials/reticlecalibration"> <img src="https://www.microscopyu.com/assets/thumbnails/_galleryThumbnailSquare/eye-piece-reticle.jpg" height="80" width="80" /> <h3>Eyepiece Reticle Calibration</h3></a> <p>Explores calibration of various eyepiece reticles using a stage micrometer and demonstrates how the reticle can then be employed to determine linear specimen dimensions.<br /></p> </li> </ul> <hr class="clear" /> <p class="viewmore"><a href="/popular-tutorials" class="button">View More</a></p> </section> <section id="authorspot"> <h2>Author Spotlight</h2> <h3>Michael Davidson</h3> <div class="authorthumb"> <img src="https://www.microscopyu.com/assets/news/_column1602x/MikeDavidson.jpg" width ="160" height="203" /> </div> <p>Michael Davidson was a brilliant scientist, artist and photographer.聽The creator of MicroscopyU,聽Davidson also聽authored many scientific articles on the subject of photomicrography.聽His photomicrographs were published in more than a thousand national and international scientific journals, popular magazines and newspapers.聽 In addition, Davidson鈥檚 photomicrography won more than 40 awards in scientific and industrial photography competitions and has been exhibited at over 50 locations nationwide.</p> <p class="learnmore"><a href="https://www.microscopyu.com/news/author-spotlight-michael-davidson" class="button">Learn more</a></p> </section> </div> <div class="rightcontent"> <section id="ads"> <div class="ad"> <h3>Nikon Small World Winners</h3> <div class="adbanner" style="background-image: url(https://www.microscopyu.com/assets/ads/SW2024_ad.jpg)"> </div> <p>Congratulations to the winners of the 2024 Nikon Small World Photomicrography Competition!<br></p> <ul><li><a class="extadlink" href="https://www.nikonsmallworld.com/galleries/2024-photomicrography-competition" target="_blank">View 2024 Winning Images</a></li><li><a class="extadlink" href="https://www.nikonsmallworld.com/galleries/2024-small-world-in-motion-competition" target="_blank">View 2024 Nikon Small World in Motion 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