HeLa Cell Histones | Nikon鈥檚 MicroscopyU

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The visible light absorption maximum of Alexa Fluor 546 is 556 nanometers and the emission maximum occurs at 573 nanometers (in the yellow region of the visible light spectrum). The primary antibody, produced from purified nuclear fractions of HeLa cells, binds specifically to an antigen determinant that is present on all five histone proteins (H1, H2A, H2B, H3, and H4). Note the strong red fluorescence intensity from both the interphase nuclei and the single prophase nucleus.</p> </div> <div class="gallerylist"> <h3>Featured in:</h3> <ul> <li><a href="https://www.microscopyu.com/galleries/fluorescence">Fluorescence Microscopy</a></li> <li><a href="https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets/yellow-excitation-filter-sets/yellow-excitation-texas-red-hyq-bandpass-emission">Yellow Excitation: Texas Red HYQ (Bandpass Emission)</a></li> </ul> </div> </div> <div class="sharebar"> <p>Share this page:</p> <ul class="share-buttons"> <li><a href="https://www.facebook.com/sharer/sharer.php?u=http%3A%2F%2Fwww.microscopyu.com&t=" title="Share on Facebook" target="_blank" onclick="window.open('https://www.facebook.com/sharer/sharer.php?u=' + encodeURIComponent(document.URL) + '&t=' + encodeURIComponent(document.URL)); return false;"><img src="/images/social_flat_rounded_rects_svg/Facebook.svg"></a></li> <li><a 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