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data-component-name="Pill" data-props="{"color":"gray","children":["Ecotoxicology"]}" data-trace="false" data-dom-id="Pill-react-component-17945cea-e1be-4415-b9f2-82f29954ce6e"></div> <div id="Pill-react-component-17945cea-e1be-4415-b9f2-82f29954ce6e"></div> </a></div></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Kanokpan Wongprasert</h3></div><div class="js-work-strip profile--work_container" data-work-id="15068666"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15068666/Lectin_Based_Profiling_of_Coelomocytes_in_Holothuria_scabra_and_Expression_of_Superoxide_Dismutase_in_Purified_Coelomocytes"><img alt="Research paper thumbnail of Lectin-Based Profiling of Coelomocytes in Holothuria scabra and Expression of Superoxide Dismutase in Purified Coelomocytes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15068666/Lectin_Based_Profiling_of_Coelomocytes_in_Holothuria_scabra_and_Expression_of_Superoxide_Dismutase_in_Purified_Coelomocytes">Lectin-Based Profiling of Coelomocytes in Holothuria scabra and Expression of Superoxide Dismutase in Purified Coelomocytes</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://mahidol.academia.edu/SomlukAsuvapongpatana">Somluk Asuvapongpatana</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/RapeepunVanichviriyakit">Rapeepun Vanichviriyakit</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BoonsirmWithyachumnarnkul">Boonsirm Withyachumnarnkul</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a></span></div><div class="wp-workCard_item"><span>Zoological science</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Coelomocytes are the first line of immune defense in marine animals. Their distributions are grea...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Coelomocytes are the first line of immune defense in marine animals. Their distributions are greatly variable even in the close animal species. In this study, we used lectin staining to aid in the classification and purification of these cells for further investigation of SOD distribution among coelomocytes of H. scraba. We classified coelomocytes into four types: type 1, lymphocytes; type 2, phagocytes; type 3, spherulocytes; and type 4, giant cells. Among four lectins used, Con A appeared to give a broad reactivity against most coelomocytes, except for giant cells. In addition, phagocytes usually engaged the highest fluorescent intensity with most lectins, with the exception of PNA, for which spherulocytes possessed the highest fluorescent intensity. Using FACS for fraction collection, it was found that F1 fraction contained the purest phagocyte population (&gt; 95%), which was highly reactive with anti- superoxide dismutase (SOD) as revealed by immunoblotting and immunofluorescen...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15068666"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15068666"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15068666; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15068666]").text(description); $(".js-view-count[data-work-id=15068666]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15068666; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15068666']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15068666, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15068666]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15068666,"title":"Lectin-Based Profiling of Coelomocytes in Holothuria scabra and Expression of Superoxide Dismutase in Purified Coelomocytes","translated_title":"","metadata":{"abstract":"Coelomocytes are the first line of immune defense in marine animals. 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In addition, phagocytes usually engaged the highest fluorescent intensity with most lectins, with the exception of PNA, for which spherulocytes possessed the highest fluorescent intensity. 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TNF-α upregulates intercellular adhesion molecule (ICAM)-1 expression on the RPE, which allows lymphocyte function-associated antigen-1 (LFA-1) to bind on leukocytes that contribute to leukocyte adhesion at sites of inflammation. Vascular endothelial growth factor (VEGF)-A(165)b is generated by alternative splicing of VEGF-A in the terminal exon, exon 8. VEGF-A(165)b is cytoprotective and antiangiogenic, but its effects on inflammation have not yet been elucidated. Therefore, we tested the hypothesis that VEGF-A(165)b regulates TNF-α-induced ICAM-1 expression and monocyte adhesion in RPE cells. Primary RPE cells were pretreated with TNF-α alone, VEGF-A(165)b alone, VEGF-A(165)b with anti-VEGF-A(165)b, or the VEGFR-2 inhibitor ZM323881 before exposure to TNF-α for 24 h. Western blotting and monocyte adhesion assays w...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8e7320df460c0aa49c67b02029966500" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431577,"asset_id":15211924,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431577/download_file?st=MTczMjc5ODg1NSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211924"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211924"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211924; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211924]").text(description); $(".js-view-count[data-work-id=15211924]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211924; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211924']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211924, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8e7320df460c0aa49c67b02029966500" } } $('.js-work-strip[data-work-id=15211924]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211924,"title":"TNF-α-induced ICAM-1 expression and monocyte adhesion in human RPE cells is mediated in part through autocrine VEGF stimulation","translated_title":"","metadata":{"abstract":"Local inflammation at the RPE cell layer is associated with inflammatory cell migration and secretion of proinflammatory cytokines such as tumor necrosis factor (TNF)-α. 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Western blotting and monocyte adhesion assays w...","publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"Molecular vision"},"translated_abstract":"Local inflammation at the RPE cell layer is associated with inflammatory cell migration and secretion of proinflammatory cytokines such as tumor necrosis factor (TNF)-α. TNF-α upregulates intercellular adhesion molecule (ICAM)-1 expression on the RPE, which allows lymphocyte function-associated antigen-1 (LFA-1) to bind on leukocytes that contribute to leukocyte adhesion at sites of inflammation. Vascular endothelial growth factor (VEGF)-A(165)b is generated by alternative splicing of VEGF-A in the terminal exon, exon 8. VEGF-A(165)b is cytoprotective and antiangiogenic, but its effects on inflammation have not yet been elucidated. Therefore, we tested the hypothesis that VEGF-A(165)b regulates TNF-α-induced ICAM-1 expression and monocyte adhesion in RPE cells. Primary RPE cells were pretreated with TNF-α alone, VEGF-A(165)b alone, VEGF-A(165)b with anti-VEGF-A(165)b, or the VEGFR-2 inhibitor ZM323881 before exposure to TNF-α for 24 h. Western blotting and monocyte adhesion assays w...","internal_url":"https://www.academia.edu/15211924/TNF_%CE%B1_induced_ICAM_1_expression_and_monocyte_adhesion_in_human_RPE_cells_is_mediated_in_part_through_autocrine_VEGF_stimulation","translated_internal_url":"","created_at":"2015-08-26T18:26:35.192-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124449,"work_id":15211924,"tagging_user_id":34271684,"tagged_user_id":37398980,"co_author_invite_id":228465,"email":"d***s@nottingham.ac.uk","display_order":0,"name":"David Bates","title":"TNF-α-induced ICAM-1 expression and monocyte adhesion in human RPE cells is mediated in part through autocrine VEGF stimulation"},{"id":5124450,"work_id":15211924,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":1153115,"email":"s***r@bristol.ac.uk","display_order":4194304,"name":"Steven Harper","title":"TNF-α-induced ICAM-1 expression and monocyte adhesion in human RPE cells is mediated in part through autocrine VEGF stimulation"}],"downloadable_attachments":[{"id":43431577,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/43431577/thumbnails/1.jpg","file_name":"TNF--induced_ICAM-1_expression_and_monoc20160306-22960-1fcujvu.pdf","download_url":"https://www.academia.edu/attachments/43431577/download_file?st=MTczMjc5ODg1NSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"TNF__induced_ICAM_1_expression_and_mono.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/43431577/TNF--induced_ICAM-1_expression_and_monoc20160306-22960-1fcujvu-libre.pdf?1457298655=\u0026response-content-disposition=attachment%3B+filename%3DTNF__induced_ICAM_1_expression_and_mono.pdf\u0026Expires=1732802455\u0026Signature=gXpn4uoxRYDc9UDCILXV6zrc4I8XSNioA54KpLxP8HQWWk3hfi3FOhUmz7WmjlVh8COJB0Qw4~uNaWm2oOwuP~UQy5SUQqVSyb6DQ~-JVnhJGUHL38UJ99HG8OstHiCnsb2r431mLdI7bRyPj~kC3uoiZk5DwBx4TGE7eo6AvaAo3Szvi2ao-o4H1I7WRBEm~V06WzAUaER~R5oHRoV4MO0k4th772xGVNf6XSBUV~QVAdH4TfYWeJbMDlViMa2sgnp4J1~8r9R7rcQBzTNoZx5BNSjx5RHR34LbTbH8aEqP5H4lPJxGN6DUuvkV1LTMpZLKilZBfm0sm26lhyuZFw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"TNF_α_induced_ICAM_1_expression_and_monocyte_adhesion_in_human_RPE_cells_is_mediated_in_part_through_autocrine_VEGF_stimulation","translated_slug":"","page_count":9,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[{"id":43431577,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/43431577/thumbnails/1.jpg","file_name":"TNF--induced_ICAM-1_expression_and_monoc20160306-22960-1fcujvu.pdf","download_url":"https://www.academia.edu/attachments/43431577/download_file?st=MTczMjc5ODg1NSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"TNF__induced_ICAM_1_expression_and_mono.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/43431577/TNF--induced_ICAM-1_expression_and_monoc20160306-22960-1fcujvu-libre.pdf?1457298655=\u0026response-content-disposition=attachment%3B+filename%3DTNF__induced_ICAM_1_expression_and_mono.pdf\u0026Expires=1732802455\u0026Signature=gXpn4uoxRYDc9UDCILXV6zrc4I8XSNioA54KpLxP8HQWWk3hfi3FOhUmz7WmjlVh8COJB0Qw4~uNaWm2oOwuP~UQy5SUQqVSyb6DQ~-JVnhJGUHL38UJ99HG8OstHiCnsb2r431mLdI7bRyPj~kC3uoiZk5DwBx4TGE7eo6AvaAo3Szvi2ao-o4H1I7WRBEm~V06WzAUaER~R5oHRoV4MO0k4th772xGVNf6XSBUV~QVAdH4TfYWeJbMDlViMa2sgnp4J1~8r9R7rcQBzTNoZx5BNSjx5RHR34LbTbH8aEqP5H4lPJxGN6DUuvkV1LTMpZLKilZBfm0sm26lhyuZFw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":10055,"name":"Cell Adhesion","url":"https://www.academia.edu/Documents/in/Cell_Adhesion"},{"id":27187,"name":"Retinal Pigment Epithelium","url":"https://www.academia.edu/Documents/in/Retinal_Pigment_Epithelium"},{"id":359001,"name":"Optometry and Ophthalmology","url":"https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology"},{"id":402972,"name":"Monocytes","url":"https://www.academia.edu/Documents/in/Monocytes"},{"id":474029,"name":"Tumor necrosis factor-alpha","url":"https://www.academia.edu/Documents/in/Tumor_necrosis_factor-alpha"},{"id":743709,"name":"Protein isoforms","url":"https://www.academia.edu/Documents/in/Protein_isoforms"},{"id":956026,"name":"Somatic Cell Count","url":"https://www.academia.edu/Documents/in/Somatic_Cell_Count"},{"id":1763968,"name":"Gene Expression Regulation","url":"https://www.academia.edu/Documents/in/Gene_Expression_Regulation"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211923"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211923/Diarylheptanoid_7_3_4_dihydroxyphenyl_5_hydroxy_1_phenyl_1E_1_heptene_from_Curcuma_comosa_Roxb_protects_retinal_pigment_epithelial_cells_against_oxidative_stress_induced_cell_death"><img alt="Research paper thumbnail of Diarylheptanoid 7-(3,4 dihydroxyphenyl)-5-hydroxy-1-phenyl-(1E)-1-heptene from Curcuma comosa Roxb. protects retinal pigment epithelial cells against oxidative stress-induced cell death" class="work-thumbnail" src="https://attachments.academia-assets.com/43431571/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211923/Diarylheptanoid_7_3_4_dihydroxyphenyl_5_hydroxy_1_phenyl_1E_1_heptene_from_Curcuma_comosa_Roxb_protects_retinal_pigment_epithelial_cells_against_oxidative_stress_induced_cell_death">Diarylheptanoid 7-(3,4 dihydroxyphenyl)-5-hydroxy-1-phenyl-(1E)-1-heptene from Curcuma comosa Roxb. protects retinal pigment epithelial cells against oxidative stress-induced cell death</a></div><div class="wp-workCard_item"><span>Toxicology in Vitro</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5bf22dd981615dcab264c02cff58a4db" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431571,"asset_id":15211923,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431571/download_file?st=MTczMjc5ODg1NSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211923"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211923"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211923; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211923]").text(description); $(".js-view-count[data-work-id=15211923]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211923; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211923']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211923, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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Anti-oxidants provide a natural defense against retinal cell damage. The present study was designed to evaluate the potential anti-oxidant activity and protective effect of two diarylheptanoids isolated from a medicinal herb Curcuma comosa; 7-(3,4 dihydroxyphenyl)-5-hydroxy-1-phenyl-(1E)-1-heptene (compound A), and 1,7-diphenyl-4(E),6(E)-heptadien-3-ol (compound B) against oxidative stress (H 2 O 2 )-induced human retinal pigment epithelial (APRE-19) cell death. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay indicated that the anti-oxidant activity (IC 50 ) of compound A was similar to that of vitamin C. Pre-treatment of ARPE-19 cells with 20 lM compound A for 4 h afforded greater protection against the insult from 500 lM H 2 O 2 , compared to a similar protection period for compound B. Compound A lowered H 2 O 2induced lipid peroxidation, malondialdehyde formation and intracellular reactive oxygen species. Furthermore, compound A ameliorated the H 2 O 2 -induced decrease in anti-oxidant enzyme activities and subsequent apoptotic cell death in ARPE-19 cells in a dose and time-dependent manner. These results suggest that compound A protects ARPE-19 cells against oxidative stress, in part, by enhancing several anti-oxidant defense mechanisms. Therefore, compound A may have therapeutic potential for diseases associated with oxidative stress, particularly degenerative retinal diseases.","publication_date":{"day":null,"month":null,"year":2011,"errors":{}},"publication_name":"Toxicology in Vitro","grobid_abstract_attachment_id":43431571},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211923/Diarylheptanoid_7_3_4_dihydroxyphenyl_5_hydroxy_1_phenyl_1E_1_heptene_from_Curcuma_comosa_Roxb_protects_retinal_pigment_epithelial_cells_against_oxidative_stress_induced_cell_death","translated_internal_url":"","created_at":"2015-08-26T18:26:35.072-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124446,"work_id":15211923,"tagging_user_id":34271684,"tagged_user_id":4453629,"co_author_invite_id":null,"email":"p***e@gmail.com","display_order":0,"name":"Pawinee 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href="https://www.academia.edu/15211922/In_vitro_protection_against_hydrogen_peroxide_induced_oxidative_stress_and_cell_death_in_ARPE_19_cells_by_Curcumin"><img alt="Research paper thumbnail of In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211922/In_vitro_protection_against_hydrogen_peroxide_induced_oxidative_stress_and_cell_death_in_ARPE_19_cells_by_Curcumin">In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin</a></div><div class="wp-workCard_item"><span>Planta Medica</span><span>, 2010</span></div><div class="wp-workCard_item 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})(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15211922]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211922,"title":"In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Planta Medica"},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211922/In_vitro_protection_against_hydrogen_peroxide_induced_oxidative_stress_and_cell_death_in_ARPE_19_cells_by_Curcumin","translated_internal_url":"","created_at":"2015-08-26T18:26:34.950-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124453,"work_id":15211922,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":1153117,"email":"y***t@mahidol.ac.th","display_order":0,"name":"Y. Kitiyanant","title":"In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin"}],"downloadable_attachments":[],"slug":"In_vitro_protection_against_hydrogen_peroxide_induced_oxidative_stress_and_cell_death_in_ARPE_19_cells_by_Curcumin","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[],"research_interests":[{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine"},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology"},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211921"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211921/Cloning_and_characterization_of_a_caspase_gene_from_black_tiger_shrimp_Penaeus_monodon_infected_with_white_spot_syndrome_virus_WSSV_"><img alt="Research paper thumbnail of Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus monodon)-infected with white spot syndrome virus (WSSV)" class="work-thumbnail" src="https://attachments.academia-assets.com/43431603/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211921/Cloning_and_characterization_of_a_caspase_gene_from_black_tiger_shrimp_Penaeus_monodon_infected_with_white_spot_syndrome_virus_WSSV_">Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus monodon)-infected with white spot syndrome virus (WSSV)</a></div><div class="wp-workCard_item"><span>Journal of Biotechnology</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="961a8d20a17362a48b83e87b09e26003" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431603,"asset_id":15211921,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431603/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211921"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211921"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211921; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211921]").text(description); $(".js-view-count[data-work-id=15211921]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211921; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211921']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211921, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "961a8d20a17362a48b83e87b09e26003" } } $('.js-work-strip[data-work-id=15211921]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211921,"title":"Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus monodon)-infected with white spot syndrome virus (WSSV)","translated_title":"","metadata":{"grobid_abstract":"A black tiger shrimp (Penaeus monodon) caspase cDNA homologue (PmCasp) has been identified from a hemocyte library using a previously identified caspase homologue from the banana shrimp (Penaeus merguiensis) as a probe. The full-length PmCasp was 1202 bp with a 954 bp open reading frame, encoding 317 amino acids. The deduced protein contained a potential active site (QACRG pentapeptide) conserved in most caspases. It had 83% identity with caspase of P. merguiensis and 30% identity with drICE protein of Drosophila melanogaster, and it exhibited caspase-3 activity in vitro. PmCasp was cloned and expressed in Escherichia coli and a rabbit polyclonal antiserum was produced. In Western blots, the antiserum reacted with purified recombinant PmCasp and with lysates of E. coli containing the expressed plasmid. In crude protein extracts from normal shrimp, the antiserum reacted with 36 and 26 kDa bands likely to correspond to inactive pro-caspase and its proteolytic intermediate form, respectively. PmCasp expression was measured in normal shrimp and in white spot syndrome virus (WSSV)-infected shrimp at 24 and 48 h post-injection (p.i.) by semi-quantitative RT-PCR, Western blot analysis, and immunohistochemistry. Semi-quantitative RT-PCR analysis revealed up-regulation of PmCasp at 48 h p.i. and expression remained high up to the moribund state. These results were supported by Western blot analysis showing increased PmCasp protein levels at 24 and 48 h p.i. when compared to normal control shrimp. Immunohistochemical analysis of gills from the WSSV-infected shrimp revealed immunoreactivity localized in the cytoplasm of both normal and apparently apoptotic cells. In summary, a caspase-3 like gene is conserved in P. monodon and is up-regulated after WSSV infection.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Journal of Biotechnology","grobid_abstract_attachment_id":43431603},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211921/Cloning_and_characterization_of_a_caspase_gene_from_black_tiger_shrimp_Penaeus_monodon_infected_with_white_spot_syndrome_virus_WSSV_","translated_internal_url":"","created_at":"2015-08-26T18:26:34.821-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124454,"work_id":15211921,"tagging_user_id":34271684,"tagged_user_id":13877169,"co_author_invite_id":null,"email":"s***0@gmail.com","display_order":0,"name":"Saengchan Senapin","title":"Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus 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blot","url":"https://www.academia.edu/Documents/in/Western_blot"},{"id":462837,"name":"Gills","url":"https://www.academia.edu/Documents/in/Gills"},{"id":585573,"name":"Amino Acid Profile","url":"https://www.academia.edu/Documents/in/Amino_Acid_Profile"},{"id":645803,"name":"Crude Protein","url":"https://www.academia.edu/Documents/in/Crude_Protein"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":844925,"name":"Open Reading Frame","url":"https://www.academia.edu/Documents/in/Open_Reading_Frame"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1181139,"name":"White Spot Syndrome Virus","url":"https://www.academia.edu/Documents/in/White_Spot_Syndrome_Virus"},{"id":1435468,"name":"Penaeidae","url":"https://www.academia.edu/Documents/in/Penaeidae"},{"id":1457054,"name":"Protein Transport","url":"https://www.academia.edu/Documents/in/Protein_Transport"},{"id":1556410,"name":"Full Length Movies","url":"https://www.academia.edu/Documents/in/Full_Length_Movies"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211920"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211920/Culture_and_development_of_the_polychaete_Perinereis_cf_nuntia"><img alt="Research paper thumbnail of Culture and development of the polychaete Perinereis cf. nuntia" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211920/Culture_and_development_of_the_polychaete_Perinereis_cf_nuntia">Culture and development of the polychaete Perinereis cf. nuntia</a></div><div class="wp-workCard_item"><span>Invertebrate Reproduction & Development</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT The polychaete Perinereis cf. nuntia, a tropical species endemic in Thailand, was cultur...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT The polychaete Perinereis cf. nuntia, a tropical species endemic in Thailand, was cultured in captivity as follows: eggs and sperm from sexually mature (epitokous) P. cf. nuntia, were artificially fertilized, and settled into a sand bed about 30 cm deep at the nectochaete stage. The sand beds were supplied with seawater at 30-ppt salinity and the nectochaetes reared for five months after which time some adults were becoming epitokes. The culture method yielded 3–4 kg polychaetes at an atokous stage per m of culture area. Because the polychaetes were to be used to feed shrimp broodstock, samples of the worms were screened for the presence of white-spot syndrome and yellow-head viruses using polymerase chain reaction (PCR) methods prior to introduction to the system as founders and at monthly intervals during their culture. Morphological details of P. cf. nuntia from fertilization to nectochaete stage are described using light microscopy, transmission (TEM) and scanning electron microscopy (SEM). The egg is surrounded by a chorion layer, TEM reveals that the microvillous tip vesicles are putative sites for the binding of the sperm to the egg surface. TEM studies of the cortical reaction show that the cortical alveoli contain a fibrous substance which, after fertilization and membrane fusion, passes into the perivitelline space to form part of the fertilization membrane, as well as being secreted from the egg surface to form a jelly layer surrounding the egg.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211920"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211920"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211920; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211920]").text(description); $(".js-view-count[data-work-id=15211920]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211920; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211920']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211920, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15211920]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211920,"title":"Culture and development of the polychaete Perinereis cf. nuntia","translated_title":"","metadata":{"abstract":"ABSTRACT The polychaete Perinereis cf. nuntia, a tropical species endemic in Thailand, was cultured in captivity as follows: eggs and sperm from sexually mature (epitokous) P. cf. nuntia, were artificially fertilized, and settled into a sand bed about 30 cm deep at the nectochaete stage. The sand beds were supplied with seawater at 30-ppt salinity and the nectochaetes reared for five months after which time some adults were becoming epitokes. The culture method yielded 3–4 kg polychaetes at an atokous stage per m of culture area. Because the polychaetes were to be used to feed shrimp broodstock, samples of the worms were screened for the presence of white-spot syndrome and yellow-head viruses using polymerase chain reaction (PCR) methods prior to introduction to the system as founders and at monthly intervals during their culture. Morphological details of P. cf. nuntia from fertilization to nectochaete stage are described using light microscopy, transmission (TEM) and scanning electron microscopy (SEM). The egg is surrounded by a chorion layer, TEM reveals that the microvillous tip vesicles are putative sites for the binding of the sperm to the egg surface. TEM studies of the cortical reaction show that the cortical alveoli contain a fibrous substance which, after fertilization and membrane fusion, passes into the perivitelline space to form part of the fertilization membrane, as well as being secreted from the egg surface to form a jelly layer surrounding the egg.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Invertebrate Reproduction \u0026 Development"},"translated_abstract":"ABSTRACT The polychaete Perinereis cf. nuntia, a tropical species endemic in Thailand, was cultured in captivity as follows: eggs and sperm from sexually mature (epitokous) P. cf. nuntia, were artificially fertilized, and settled into a sand bed about 30 cm deep at the nectochaete stage. The sand beds were supplied with seawater at 30-ppt salinity and the nectochaetes reared for five months after which time some adults were becoming epitokes. The culture method yielded 3–4 kg polychaetes at an atokous stage per m of culture area. Because the polychaetes were to be used to feed shrimp broodstock, samples of the worms were screened for the presence of white-spot syndrome and yellow-head viruses using polymerase chain reaction (PCR) methods prior to introduction to the system as founders and at monthly intervals during their culture. Morphological details of P. cf. nuntia from fertilization to nectochaete stage are described using light microscopy, transmission (TEM) and scanning electron microscopy (SEM). The egg is surrounded by a chorion layer, TEM reveals that the microvillous tip vesicles are putative sites for the binding of the sperm to the egg surface. TEM studies of the cortical reaction show that the cortical alveoli contain a fibrous substance which, after fertilization and membrane fusion, passes into the perivitelline space to form part of the fertilization membrane, as well as being secreted from the egg surface to form a jelly layer surrounding the egg.","internal_url":"https://www.academia.edu/15211920/Culture_and_development_of_the_polychaete_Perinereis_cf_nuntia","translated_internal_url":"","created_at":"2015-08-26T18:26:34.691-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124452,"work_id":15211920,"tagging_user_id":34271684,"tagged_user_id":35784640,"co_author_invite_id":1063008,"email":"p***b@mahidol.ac.th","display_order":0,"name":"Prasert Sobhon","title":"Culture and development of the polychaete Perinereis cf. nuntia"}],"downloadable_attachments":[],"slug":"Culture_and_development_of_the_polychaete_Perinereis_cf_nuntia","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":10655,"name":"Scanning Electron Microscopy","url":"https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction"},{"id":122189,"name":"Membrane Fusion","url":"https://www.academia.edu/Documents/in/Membrane_Fusion"},{"id":541736,"name":"Sexual maturity","url":"https://www.academia.edu/Documents/in/Sexual_maturity"},{"id":885893,"name":"Light microscopy","url":"https://www.academia.edu/Documents/in/Light_microscopy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15068663"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15068663/Solvent_extracts_of_the_red_seaweed_Gracilaria_fisheri_prevent_Vibrio_harveyi_infections_in_the_black_tiger_shrimp_Penaeus_monodon"><img alt="Research paper thumbnail of Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15068663/Solvent_extracts_of_the_red_seaweed_Gracilaria_fisheri_prevent_Vibrio_harveyi_infections_in_the_black_tiger_shrimp_Penaeus_monodon">Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://mahidol.academia.edu/SomlukAsuvapongpatana">Somluk Asuvapongpatana</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BoonsirmWithyachumnarnkul">Boonsirm Withyachumnarnkul</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a></span></div><div class="wp-workCard_item"><span>Fish & Shellfish Immunology</span><span>, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shrimp. Since compounds from seaweed have been reported to have anti-bacterial and immunostimulant activity, this study was conducted to determine whether solvent extracts from the red seaweed Gracilaria fisheri might be a possible alternative for prevention and treatment of shrimp vibriosis caused by Vibrio harveyi. Seaweed extracts prepared using ethanol, methanol, chloroform and hexane were evaluated for anti-V. harveyi activity by the disc-diffusion method. The ethanol, methanol and chloroform extracts showed activity against a virulent strain of V. harveyi with potency (minimal inhibitory concentrations in the range of 90-190 μg ml(-1)) equivalent to the antibiotic norfloxacin. The ethanol extract was not toxic to the brine shrimp Artemia salina when it was fed to them for enrichment prior to their use, in turn, as feed for postlarvae of Penaeus monodon. Postlarvae fed with these enriched Artemia gave significantly lower mortality than control postlarvae after challenge with V. harveyi. In addition, P. monodon juveniles injected with the ethanol extract showed a significant increase in the total number of haemocytes and an increased proportion of semi-granulocytes and granulocytes when compared to control shrimp. The activities of phenoloxidase and superoxide dismutase were also increased, with an accompanying increase in superoxide anion production. When these juvenile shrimp were challenged with V. harveyi, mortality was markedly reduced compared to that of control shrimp. The results indicated that ethanol extracts of G. fisheri had immunostimulant and antimicrobial activity that could protect P. monodon against V. harveyi.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15068663"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15068663"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15068663; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15068663]").text(description); $(".js-view-count[data-work-id=15068663]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15068663; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15068663']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15068663, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15068663]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15068663,"title":"Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon","translated_title":"","metadata":{"abstract":"Vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shrimp. Since compounds from seaweed have been reported to have anti-bacterial and immunostimulant activity, this study was conducted to determine whether solvent extracts from the red seaweed Gracilaria fisheri might be a possible alternative for prevention and treatment of shrimp vibriosis caused by Vibrio harveyi. Seaweed extracts prepared using ethanol, methanol, chloroform and hexane were evaluated for anti-V. harveyi activity by the disc-diffusion method. The ethanol, methanol and chloroform extracts showed activity against a virulent strain of V. harveyi with potency (minimal inhibitory concentrations in the range of 90-190 μg ml(-1)) equivalent to the antibiotic norfloxacin. The ethanol extract was not toxic to the brine shrimp Artemia salina when it was fed to them for enrichment prior to their use, in turn, as feed for postlarvae of Penaeus monodon. Postlarvae fed with these enriched Artemia gave significantly lower mortality than control postlarvae after challenge with V. harveyi. In addition, P. monodon juveniles injected with the ethanol extract showed a significant increase in the total number of haemocytes and an increased proportion of semi-granulocytes and granulocytes when compared to control shrimp. The activities of phenoloxidase and superoxide dismutase were also increased, with an accompanying increase in superoxide anion production. When these juvenile shrimp were challenged with V. harveyi, mortality was markedly reduced compared to that of control shrimp. The results indicated that ethanol extracts of G. fisheri had immunostimulant and antimicrobial activity that could protect P. monodon against V. harveyi.","publication_date":{"day":null,"month":null,"year":2011,"errors":{}},"publication_name":"Fish \u0026 Shellfish Immunology"},"translated_abstract":"Vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shrimp. Since compounds from seaweed have been reported to have anti-bacterial and immunostimulant activity, this study was conducted to determine whether solvent extracts from the red seaweed Gracilaria fisheri might be a possible alternative for prevention and treatment of shrimp vibriosis caused by Vibrio harveyi. Seaweed extracts prepared using ethanol, methanol, chloroform and hexane were evaluated for anti-V. harveyi activity by the disc-diffusion method. The ethanol, methanol and chloroform extracts showed activity against a virulent strain of V. harveyi with potency (minimal inhibitory concentrations in the range of 90-190 μg ml(-1)) equivalent to the antibiotic norfloxacin. The ethanol extract was not toxic to the brine shrimp Artemia salina when it was fed to them for enrichment prior to their use, in turn, as feed for postlarvae of Penaeus monodon. Postlarvae fed with these enriched Artemia gave significantly lower mortality than control postlarvae after challenge with V. harveyi. In addition, P. monodon juveniles injected with the ethanol extract showed a significant increase in the total number of haemocytes and an increased proportion of semi-granulocytes and granulocytes when compared to control shrimp. The activities of phenoloxidase and superoxide dismutase were also increased, with an accompanying increase in superoxide anion production. When these juvenile shrimp were challenged with V. harveyi, mortality was markedly reduced compared to that of control shrimp. The results indicated that ethanol extracts of G. fisheri had immunostimulant and antimicrobial activity that could protect P. monodon against V. harveyi.","internal_url":"https://www.academia.edu/15068663/Solvent_extracts_of_the_red_seaweed_Gracilaria_fisheri_prevent_Vibrio_harveyi_infections_in_the_black_tiger_shrimp_Penaeus_monodon","translated_internal_url":"","created_at":"2015-08-21T00:50:32.621-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34098982,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":4880142,"work_id":15068663,"tagging_user_id":34098982,"tagged_user_id":34291986,"co_author_invite_id":1108791,"email":"w***m@yahoo.com","display_order":0,"name":"Boonsirm Withyachumnarnkul","title":"Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon"},{"id":4880147,"work_id":15068663,"tagging_user_id":34098982,"tagged_user_id":34271684,"co_author_invite_id":1108792,"email":"k***n@mahidol.ac.th","display_order":4194304,"name":"Kanokpan Wongprasert","title":"Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon"},{"id":4880149,"work_id":15068663,"tagging_user_id":34098982,"tagged_user_id":null,"co_author_invite_id":1108793,"email":"k***5@gmail.com","display_order":6291456,"name":"Kulwadee Kanjana","title":"Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon"}],"downloadable_attachments":[],"slug":"Solvent_extracts_of_the_red_seaweed_Gracilaria_fisheri_prevent_Vibrio_harveyi_infections_in_the_black_tiger_shrimp_Penaeus_monodon","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34098982,"first_name":"Somluk","middle_initials":null,"last_name":"Asuvapongpatana","page_name":"SomlukAsuvapongpatana","domain_name":"mahidol","created_at":"2015-08-21T00:49:58.011-07:00","display_name":"Somluk Asuvapongpatana","url":"https://mahidol.academia.edu/SomlukAsuvapongpatana"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":8058,"name":"Artemia","url":"https://www.academia.edu/Documents/in/Artemia"},{"id":80451,"name":"Solvent Extraction","url":"https://www.academia.edu/Documents/in/Solvent_Extraction"},{"id":111007,"name":"Antimicrobial activity","url":"https://www.academia.edu/Documents/in/Antimicrobial_activity"},{"id":127957,"name":"Penaeus Monodon","url":"https://www.academia.edu/Documents/in/Penaeus_Monodon"},{"id":170652,"name":"Fisheries Sciences","url":"https://www.academia.edu/Documents/in/Fisheries_Sciences"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts"},{"id":379748,"name":"Vibrio","url":"https://www.academia.edu/Documents/in/Vibrio"},{"id":442493,"name":"Larva","url":"https://www.academia.edu/Documents/in/Larva"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":743643,"name":"Host Pathogen Interactions","url":"https://www.academia.edu/Documents/in/Host_Pathogen_Interactions"},{"id":1035964,"name":"Superoxide Anion","url":"https://www.academia.edu/Documents/in/Superoxide_Anion"},{"id":1435468,"name":"Penaeidae","url":"https://www.academia.edu/Documents/in/Penaeidae"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211919"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211919/Withering_syndrome_in_the_abalone_Haliotis_diversicolor_supertexta"><img alt="Research paper thumbnail of Withering syndrome in the abalone Haliotis diversicolor supertexta" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211919/Withering_syndrome_in_the_abalone_Haliotis_diversicolor_supertexta">Withering syndrome in the abalone Haliotis diversicolor supertexta</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://uw-us.academia.edu/CarolynFriedman">Carolyn Friedman</a></span></div><div class="wp-workCard_item"><span>Diseases of Aquatic Organisms</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic H...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic Haliotis diversicolor supertexta and the native H. asinina. Withering syndrome (WS) in abalone is caused by an infection with the Rickettsia-like organism (RLO) &amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Candidatus Xenohaliotis californiensis&amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; and has been spread to many countries globally. The present study reports the first observation of the WS-RLO agent in the small abalone, H. diversicolor supertexta in Thailand, Taiwan (ROC) and the People&amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s Republic of China (PRC). Under light microscopy, the RLO was observed as intracytoplasmic inclusions within epithelial cells lining the post-esophagus and, to a minor extent, the intestine of H. diversicolor. Under transmission electron microscopy, inclusions were characterized as colonies of rod-shaped bacteria, 200 x 1800 nm in size, within a vesicle in the cytoplasm of the infected cell. The RLO from the small abalone bound with WS-RLO-specific in situ hybridization probes and was amplified by polymerase chain reaction (PCR), using primers designed from the 16S rDNA sequence of the original WS-RLO from California, USA. The PCR product of RLO samples from both the PRC and Thailand showed extremely high identity with the California WS-RLO (100 and 99%, respectively). These data combined with the history of abalone movements for aquaculture purposes indicate that RLOs observed in Thailand, Taiwan and the PRC are the WS-RLO that originated from California.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211919"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211919"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211919; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211919]").text(description); $(".js-view-count[data-work-id=15211919]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211919; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211919']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211919, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15211919]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211919,"title":"Withering syndrome in the abalone Haliotis diversicolor supertexta","translated_title":"","metadata":{"abstract":"Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic Haliotis diversicolor supertexta and the native H. asinina. Withering syndrome (WS) in abalone is caused by an infection with the Rickettsia-like organism (RLO) \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Candidatus Xenohaliotis californiensis\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; and has been spread to many countries globally. The present study reports the first observation of the WS-RLO agent in the small abalone, H. diversicolor supertexta in Thailand, Taiwan (ROC) and the People\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s Republic of China (PRC). Under light microscopy, the RLO was observed as intracytoplasmic inclusions within epithelial cells lining the post-esophagus and, to a minor extent, the intestine of H. diversicolor. Under transmission electron microscopy, inclusions were characterized as colonies of rod-shaped bacteria, 200 x 1800 nm in size, within a vesicle in the cytoplasm of the infected cell. The RLO from the small abalone bound with WS-RLO-specific in situ hybridization probes and was amplified by polymerase chain reaction (PCR), using primers designed from the 16S rDNA sequence of the original WS-RLO from California, USA. The PCR product of RLO samples from both the PRC and Thailand showed extremely high identity with the California WS-RLO (100 and 99%, respectively). These data combined with the history of abalone movements for aquaculture purposes indicate that RLOs observed in Thailand, Taiwan and the PRC are the WS-RLO that originated from California.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Diseases of Aquatic Organisms"},"translated_abstract":"Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic Haliotis diversicolor supertexta and the native H. asinina. Withering syndrome (WS) in abalone is caused by an infection with the Rickettsia-like organism (RLO) \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Candidatus Xenohaliotis californiensis\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; and has been spread to many countries globally. The present study reports the first observation of the WS-RLO agent in the small abalone, H. diversicolor supertexta in Thailand, Taiwan (ROC) and the People\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s Republic of China (PRC). Under light microscopy, the RLO was observed as intracytoplasmic inclusions within epithelial cells lining the post-esophagus and, to a minor extent, the intestine of H. diversicolor. Under transmission electron microscopy, inclusions were characterized as colonies of rod-shaped bacteria, 200 x 1800 nm in size, within a vesicle in the cytoplasm of the infected cell. The RLO from the small abalone bound with WS-RLO-specific in situ hybridization probes and was amplified by polymerase chain reaction (PCR), using primers designed from the 16S rDNA sequence of the original WS-RLO from California, USA. The PCR product of RLO samples from both the PRC and Thailand showed extremely high identity with the California WS-RLO (100 and 99%, respectively). These data combined with the history of abalone movements for aquaculture purposes indicate that RLOs observed in Thailand, Taiwan and the PRC are the WS-RLO that originated from California.","internal_url":"https://www.academia.edu/15211919/Withering_syndrome_in_the_abalone_Haliotis_diversicolor_supertexta","translated_internal_url":"","created_at":"2015-08-26T18:26:34.484-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124447,"work_id":15211919,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":256290,"email":"c***f@u.washington.edu","display_order":0,"name":"Carolyn Friedman","title":"Withering syndrome in the abalone Haliotis diversicolor supertexta"},{"id":5124448,"work_id":15211919,"tagging_user_id":34271684,"tagged_user_id":42296411,"co_author_invite_id":256291,"email":"c***f@uw.edu","affiliation":"UW","display_order":4194304,"name":"Carolyn Friedman","title":"Withering syndrome in the abalone Haliotis diversicolor supertexta"}],"downloadable_attachments":[],"slug":"Withering_syndrome_in_the_abalone_Haliotis_diversicolor_supertexta","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[],"research_interests":[{"id":23848,"name":"Aquaculture","url":"https://www.academia.edu/Documents/in/Aquaculture"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":85919,"name":"Gastropoda","url":"https://www.academia.edu/Documents/in/Gastropoda"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":1288215,"name":"Rickettsia","url":"https://www.academia.edu/Documents/in/Rickettsia"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15068662"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15068662/Adaptation_of_the_black_tiger_shrimp_Penaeus_monodon_to_different_salinities_through_an_excretory_function_of_the_antennal_gland"><img alt="Research paper thumbnail of Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15068662/Adaptation_of_the_black_tiger_shrimp_Penaeus_monodon_to_different_salinities_through_an_excretory_function_of_the_antennal_gland">Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://mahidol.academia.edu/SomlukAsuvapongpatana">Somluk Asuvapongpatana</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BoonsirmWithyachumnarnkul">Boonsirm Withyachumnarnkul</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a></span></div><div class="wp-workCard_item"><span>Cell and Tissue Research</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinit...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular adaptation of their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans. We have investigated the morphological and biochemical alterations of the antennal glands in shrimp reared in 7, 15, or 30 ppt seawater. Drastic changes occur in animals reared under 7 ppt conditions. Ultrastructural studies of the antennal gland in shrimps reared in 7 ppt seawater have revealed that podocytic cells in the coelomosacs ramify with more cytoplasmic processes forming the filtration slits, and that the tubular labyrinth cells possess more mitochondria in their basal striation and a wider tubular lumen than those found in the other groups. Many apical cytoplasmic blebs from labyrinth cells have also been seen in the lumen of the labyrinths under 7 ppt conditions, a feature that is not as prominent under the other conditions. The expression and activity of the Na(+)/K(+)-ATPase in the antennal gland are also correlated with the surrounding environment: the lower the salinity, the higher the expression and activity of the enzyme. Immunohistochemistry results have demonstrated the highest staining intensity in the labyrinth cells of shrimps reared under 7 ppt conditions. Our findings thus suggest that one of the adaptation mechanisms of this shrimp to the surrounding salinity is the regulation of Na(+)/K(+)-ATPase expression in the antennal gland, in conjunction with subcellular changes in its excretory cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15068662"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15068662"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15068662; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15068662]").text(description); $(".js-view-count[data-work-id=15068662]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15068662; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15068662']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15068662, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15068662]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15068662,"title":"Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland","translated_title":"","metadata":{"abstract":"Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular adaptation of their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans. We have investigated the morphological and biochemical alterations of the antennal glands in shrimp reared in 7, 15, or 30 ppt seawater. Drastic changes occur in animals reared under 7 ppt conditions. Ultrastructural studies of the antennal gland in shrimps reared in 7 ppt seawater have revealed that podocytic cells in the coelomosacs ramify with more cytoplasmic processes forming the filtration slits, and that the tubular labyrinth cells possess more mitochondria in their basal striation and a wider tubular lumen than those found in the other groups. Many apical cytoplasmic blebs from labyrinth cells have also been seen in the lumen of the labyrinths under 7 ppt conditions, a feature that is not as prominent under the other conditions. The expression and activity of the Na(+)/K(+)-ATPase in the antennal gland are also correlated with the surrounding environment: the lower the salinity, the higher the expression and activity of the enzyme. Immunohistochemistry results have demonstrated the highest staining intensity in the labyrinth cells of shrimps reared under 7 ppt conditions. Our findings thus suggest that one of the adaptation mechanisms of this shrimp to the surrounding salinity is the regulation of Na(+)/K(+)-ATPase expression in the antennal gland, in conjunction with subcellular changes in its excretory cells.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Cell and Tissue Research"},"translated_abstract":"Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular adaptation of their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans. We have investigated the morphological and biochemical alterations of the antennal glands in shrimp reared in 7, 15, or 30 ppt seawater. Drastic changes occur in animals reared under 7 ppt conditions. Ultrastructural studies of the antennal gland in shrimps reared in 7 ppt seawater have revealed that podocytic cells in the coelomosacs ramify with more cytoplasmic processes forming the filtration slits, and that the tubular labyrinth cells possess more mitochondria in their basal striation and a wider tubular lumen than those found in the other groups. Many apical cytoplasmic blebs from labyrinth cells have also been seen in the lumen of the labyrinths under 7 ppt conditions, a feature that is not as prominent under the other conditions. The expression and activity of the Na(+)/K(+)-ATPase in the antennal gland are also correlated with the surrounding environment: the lower the salinity, the higher the expression and activity of the enzyme. Immunohistochemistry results have demonstrated the highest staining intensity in the labyrinth cells of shrimps reared under 7 ppt conditions. Our findings thus suggest that one of the adaptation mechanisms of this shrimp to the surrounding salinity is the regulation of Na(+)/K(+)-ATPase expression in the antennal gland, in conjunction with subcellular changes in its excretory cells.","internal_url":"https://www.academia.edu/15068662/Adaptation_of_the_black_tiger_shrimp_Penaeus_monodon_to_different_salinities_through_an_excretory_function_of_the_antennal_gland","translated_internal_url":"","created_at":"2015-08-21T00:50:32.520-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34098982,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":4880135,"work_id":15068662,"tagging_user_id":34098982,"tagged_user_id":null,"co_author_invite_id":1089797,"email":"s***y@mahidol.ac.th","display_order":0,"name":"Wattana Weerachatyanukul","title":"Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland"},{"id":4880143,"work_id":15068662,"tagging_user_id":34098982,"tagged_user_id":34291986,"co_author_invite_id":1108791,"email":"w***m@yahoo.com","display_order":4194304,"name":"Boonsirm Withyachumnarnkul","title":"Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland"},{"id":4880148,"work_id":15068662,"tagging_user_id":34098982,"tagged_user_id":34271684,"co_author_invite_id":1108792,"email":"k***n@mahidol.ac.th","display_order":6291456,"name":"Kanokpan Wongprasert","title":"Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland"}],"downloadable_attachments":[],"slug":"Adaptation_of_the_black_tiger_shrimp_Penaeus_monodon_to_different_salinities_through_an_excretory_function_of_the_antennal_gland","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34098982,"first_name":"Somluk","middle_initials":null,"last_name":"Asuvapongpatana","page_name":"SomlukAsuvapongpatana","domain_name":"mahidol","created_at":"2015-08-21T00:49:58.011-07:00","display_name":"Somluk Asuvapongpatana","url":"https://mahidol.academia.edu/SomlukAsuvapongpatana"},"attachments":[],"research_interests":[{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry"},{"id":82107,"name":"Salinity","url":"https://www.academia.edu/Documents/in/Salinity"},{"id":127957,"name":"Penaeus Monodon","url":"https://www.academia.edu/Documents/in/Penaeus_Monodon"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme"},{"id":1435468,"name":"Penaeidae","url":"https://www.academia.edu/Documents/in/Penaeidae"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211918"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211918/Development_of_two_microsatellite_multiplex_systems_for_black_tiger_shrimp_Penaeus_monodon_and_its_application_in_genetic_diversity_study_for_two_populations"><img alt="Research paper thumbnail of Development of two microsatellite multiplex systems for black tiger shrimp Penaeus monodon and its application in genetic diversity study for two populations" class="work-thumbnail" src="https://attachments.academia-assets.com/43431597/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211918/Development_of_two_microsatellite_multiplex_systems_for_black_tiger_shrimp_Penaeus_monodon_and_its_application_in_genetic_diversity_study_for_two_populations">Development of two microsatellite multiplex systems for black tiger shrimp Penaeus monodon and its application in genetic diversity study for two populations</a></div><div class="wp-workCard_item"><span>Aquaculture</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3d389a49559718f33ec797a0a0f40af6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431597,"asset_id":15211918,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431597/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211918"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211918"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211918; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211918]").text(description); $(".js-view-count[data-work-id=15211918]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211918; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211918']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211918, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3d389a49559718f33ec797a0a0f40af6" } } $('.js-work-strip[data-work-id=15211918]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211918,"title":"Development of two microsatellite multiplex systems for black tiger shrimp Penaeus monodon and its application in genetic diversity study for two populations","translated_title":"","metadata":{"grobid_abstract":"Despite large numbers of putative microsatellites currently listed in databases for Penaeus monodon, there are no publications on assessing these markers for multiplexed high throughput systems either for fingerprinting or population genetics study purposes in P. monodon. Accordingly, we started our investigation on the development of high throughput systems for P. monodon. Ninety publicly-available P. monodon microsatellite sequences were initially screened for suitability. They were assessed for the presence of tri-or tetra-nucleotide repeats, repeat number and type, suitability of flanking sequences for primer design and estimated size of product (100 to 350 bp). Nineteen sequences were chosen for preliminary assessment on a panel of 15 animals. Of the 19 tested, only 12 were suitable for further investigation. Therefore a 2-step enrichment library approach was adopted to develop additional microsatellites. Of 42 new unique microsatellite sequences obtained, eight sequences were assessed and seven showed polymorphism. Together, these 19 markers were examined further for their ease of amplification and reliability of allele calling for inclusion in high throughput systems. Thirteen polymorphic markers were incorporated into two multiplex systems (six and seven markers, respectively). These multiplexed systems were then used to evaluate the genetic diversity between two populations of P. monodon, one from the East Coast of Australia and a single pond containing farmed animals from Thailand. There were significant differences between the two populations. Three markers in system 1 showed Hardy-Weinberg disequilibrium in both populations, indicating their unsuitability as high throughput system markers. Using two systems and the UPGMA clustering methods revealed the existence of sub-populations within the Australian wild population. The results indicate the usefulness of the two multiplexed microsatellite systems in genetic diversity studies. Crown","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Aquaculture","grobid_abstract_attachment_id":43431597},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211918/Development_of_two_microsatellite_multiplex_systems_for_black_tiger_shrimp_Penaeus_monodon_and_its_application_in_genetic_diversity_study_for_two_populations","translated_internal_url":"","created_at":"2015-08-26T18:26:33.753-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124445,"work_id":15211918,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":1004784,"email":"j***s@csiro.au","display_order":0,"name":"Jennifer Meadows","title":"Development of two microsatellite multiplex systems for black tiger shrimp Penaeus monodon and its application in genetic diversity study for two 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211917"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211917/Retinopathy_in_stunted_black_tiger_shrimp_Penaeus_monodon_and_possible_association_with_Laem_Singh_virus_LSNV_"><img alt="Research paper thumbnail of Retinopathy in stunted black tiger shrimp Penaeus monodon and possible association with Laem-Singh virus (LSNV)" class="work-thumbnail" src="https://attachments.academia-assets.com/43431585/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211917/Retinopathy_in_stunted_black_tiger_shrimp_Penaeus_monodon_and_possible_association_with_Laem_Singh_virus_LSNV_">Retinopathy in stunted black tiger shrimp Penaeus monodon and possible association with Laem-Singh virus (LSNV)</a></div><div class="wp-workCard_item"><span>Aquaculture</span><span>, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="086bb30218c78ff490c88f1b629a9c85" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431585,"asset_id":15211917,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431585/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span 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_.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "086bb30218c78ff490c88f1b629a9c85" } } $('.js-work-strip[data-work-id=15211917]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211917,"title":"Retinopathy in stunted black tiger shrimp Penaeus monodon and possible association with Laem-Singh virus (LSNV)","translated_title":"","metadata":{"grobid_abstract":"Monodon slow-growth syndrome (MSGS) is a condition of Penaeus monodon cultivation ponds characterized by abnormally slow growth and coefficients of size variation greater than 35%. Shrimp from these ponds often show the presence Laem-Singh virus (LSNV) in the lymphoid organ, heart and other tissues. We compared various tissues of abnormally small and normal size (large) P. monodon from 3 MSGS ponds and from 2 reference ponds with normal growth (control ponds) both histologically and for the presence of LSNV. Small and large shrimp from the MSGS ponds were positive for LSNV by reverse transcriptase polymerase chain reaction (RT-PCR) tests while shrimp from one normal-growth pond were positive and from another negative. The LSNV infections were confirmed by transmission electron microscopy (TEM) and in situ hybridization (ISH). The only striking difference between the small and large LSNV-positive shrimp was retinopathy exclusively in the small shrimp from the MSGS ponds. Retinopathy comprised abnormally enlarged haemolymphatic vessels, haemocytic infiltration and rupture of the membrane that separated the fasciculated zone from the overlying row of retinular cells. By TEM and ISH, LSNV was detected in the fasciculated zone and in onion bodies of the organ of Bellonci in the small shrimp from the MSGS ponds, but not in those tissues and cells of the large shrimp from the MSGS pond or from the normal-growth ponds, whether LSNV-positive or not. The results suggested that retinopathy associated with LSNV may be linked causally to stunting of P. monodon in MSGS ponds.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Aquaculture","grobid_abstract_attachment_id":43431585},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211917/Retinopathy_in_stunted_black_tiger_shrimp_Penaeus_monodon_and_possible_association_with_Laem_Singh_virus_LSNV_","translated_internal_url":"","created_at":"2015-08-26T18:26:33.041-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124451,"work_id":15211917,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":1153116,"email":"s***w@gmail.com","display_order":0,"name":"Waraporn Sakaew","title":"Retinopathy in stunted black tiger shrimp Penaeus monodon and possible association with Laem-Singh virus (LSNV)"}],"downloadable_attachments":[{"id":43431585,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/43431585/thumbnails/1.jpg","file_name":"j.aquaculture.2008.07.040.pdf20160306-22960-1eui2x6","download_url":"https://www.academia.edu/attachments/43431585/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Retinopathy_in_stunted_black_tiger_shrim.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/43431585/j.aquaculture.2008.07.040-libre.pdf20160306-22960-1eui2x6?1457298651=\u0026response-content-disposition=attachment%3B+filename%3DRetinopathy_in_stunted_black_tiger_shrim.pdf\u0026Expires=1732802456\u0026Signature=PL-BajW-RadVJ9fQus80y1E6J542Uerx9ctLaLv19PyOIGe8LpvgFK84aYAA4GKdXBLtav-RdE9LgY5aEXWOq-2Wz6c2atX9FSmP3XkWhl-ockWqHnNwZMtj7qDmNLI-9SG9j9s5NCauPzIToEavUzBiY5u49cEUpIObHIEtMt74t67gMMn2OBzyUU1yG4x1ItOvI8xfXV3P0OzvtLQ9lE7sngifPN2OSeRsxJRRVzQGjfKcvpeZ-MAlvX6hCMi7NUQbh4-AKgxL5KSy3XK2kVTqaU-FeYKttyRqiO-Fz6yz7C513hTV3xZWT44FW9sKlP2Prvno3sBtoinZODHirA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Retinopathy_in_stunted_black_tiger_shrimp_Penaeus_monodon_and_possible_association_with_Laem_Singh_virus_LSNV_","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[{"id":43431585,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/43431585/thumbnails/1.jpg","file_name":"j.aquaculture.2008.07.040.pdf20160306-22960-1eui2x6","download_url":"https://www.academia.edu/attachments/43431585/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Retinopathy_in_stunted_black_tiger_shrim.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/43431585/j.aquaculture.2008.07.040-libre.pdf20160306-22960-1eui2x6?1457298651=\u0026response-content-disposition=attachment%3B+filename%3DRetinopathy_in_stunted_black_tiger_shrim.pdf\u0026Expires=1732802456\u0026Signature=PL-BajW-RadVJ9fQus80y1E6J542Uerx9ctLaLv19PyOIGe8LpvgFK84aYAA4GKdXBLtav-RdE9LgY5aEXWOq-2Wz6c2atX9FSmP3XkWhl-ockWqHnNwZMtj7qDmNLI-9SG9j9s5NCauPzIToEavUzBiY5u49cEUpIObHIEtMt74t67gMMn2OBzyUU1yG4x1ItOvI8xfXV3P0OzvtLQ9lE7sngifPN2OSeRsxJRRVzQGjfKcvpeZ-MAlvX6hCMi7NUQbh4-AKgxL5KSy3XK2kVTqaU-FeYKttyRqiO-Fz6yz7C513hTV3xZWT44FW9sKlP2Prvno3sBtoinZODHirA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":7049,"name":"Crustacea","url":"https://www.academia.edu/Documents/in/Crustacea"},{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy"},{"id":23848,"name":"Aquaculture","url":"https://www.academia.edu/Documents/in/Aquaculture"},{"id":82145,"name":"Virus","url":"https://www.academia.edu/Documents/in/Virus"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction"},{"id":127957,"name":"Penaeus Monodon","url":"https://www.academia.edu/Documents/in/Penaeus_Monodon"},{"id":170652,"name":"Fisheries Sciences","url":"https://www.academia.edu/Documents/in/Fisheries_Sciences"},{"id":409570,"name":"Onion","url":"https://www.academia.edu/Documents/in/Onion"},{"id":973494,"name":"Reverse Transcriptase","url":"https://www.academia.edu/Documents/in/Reverse_Transcriptase"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15068660"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15068660/Serotonin_stimulates_ovarian_maturation_and_spawning_in_the_black_tiger_shrimp_Penaeus_monodon"><img alt="Research paper thumbnail of Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon" class="work-thumbnail" src="https://attachments.academia-assets.com/43605089/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15068660/Serotonin_stimulates_ovarian_maturation_and_spawning_in_the_black_tiger_shrimp_Penaeus_monodon">Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://mahidol.academia.edu/SomlukAsuvapongpatana">Somluk Asuvapongpatana</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BoonsirmWithyachumnarnkul">Boonsirm Withyachumnarnkul</a></span></div><div class="wp-workCard_item"><span>Aquaculture</span><span>, 2006</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="be54b20d7da50141471a3f75b3beb0b5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43605089,"asset_id":15068660,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43605089/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15068660"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15068660"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15068660; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "be54b20d7da50141471a3f75b3beb0b5" } } $('.js-work-strip[data-work-id=15068660]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15068660,"title":"Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon","translated_title":"","metadata":{"grobid_abstract":"Serotonin (5-hydroxytryptamine, 5HT) has been reported to induce ovarian maturation and spawning in the crayfish Procambarus clarkii and white Pacific shrimp Litopenaeus vannamei. The aim of this study was to explore the role of exogenous 5HT on the reproductive performance of the black tiger shrimp Penaeus monodon. 5HT solution was injected into domesticated P. monodon broodstock at 50 μg/g body weight and ovarian maturation and spawning were recorded. The presence of 5HT in the ovary and oviduct of P. monodon was also studied by immunohistochemistry and its levels in the ovary by enzyme link immunoabsorbance assay (ELISA). The 5HT-injected P. monodon developed ovarian maturation and spawning rate at the level comparable to that of unilateral eyestalk-ablated shrimp. Hatching rate and the amount of nauplii produced per spawner were also significantly higher in the 5HT-injected shrimp, compared to the eyestalk-ablated shrimp. 5HT-positive reactions were found in the follicular cells of pre-vitellogenic oocytes, in the cytoplasm of early vitellogenic oocytes and on the cell membrane and cytoplasm of late vitellogenic oocytes. 5HT in the ovary was present at 3.53 ± 0.26 ng/mg protein level in previtellogenic stage and increased to 17.03 ± 0.57 ng/mg protein level in the mature stage of the ovary. The results suggest a significant role of 5HT, possibly directly on the ovary and oviduct, on the reproductive function of female P. monodon.","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Aquaculture","grobid_abstract_attachment_id":43605089},"translated_abstract":null,"internal_url":"https://www.academia.edu/15068660/Serotonin_stimulates_ovarian_maturation_and_spawning_in_the_black_tiger_shrimp_Penaeus_monodon","translated_internal_url":"","created_at":"2015-08-21T00:50:32.327-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34098982,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":4880151,"work_id":15068660,"tagging_user_id":34098982,"tagged_user_id":34271684,"co_author_invite_id":1108792,"email":"k***n@mahidol.ac.th","display_order":0,"name":"Kanokpan Wongprasert","title":"Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus 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class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15068666/Lectin_Based_Profiling_of_Coelomocytes_in_Holothuria_scabra_and_Expression_of_Superoxide_Dismutase_in_Purified_Coelomocytes"><img alt="Research paper thumbnail of Lectin-Based Profiling of Coelomocytes in Holothuria scabra and Expression of Superoxide Dismutase in Purified Coelomocytes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15068666/Lectin_Based_Profiling_of_Coelomocytes_in_Holothuria_scabra_and_Expression_of_Superoxide_Dismutase_in_Purified_Coelomocytes">Lectin-Based Profiling of Coelomocytes in Holothuria scabra and Expression of Superoxide Dismutase in Purified Coelomocytes</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://mahidol.academia.edu/SomlukAsuvapongpatana">Somluk Asuvapongpatana</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/RapeepunVanichviriyakit">Rapeepun Vanichviriyakit</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BoonsirmWithyachumnarnkul">Boonsirm Withyachumnarnkul</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a></span></div><div class="wp-workCard_item"><span>Zoological science</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Coelomocytes are the first line of immune defense in marine animals. Their distributions are grea...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Coelomocytes are the first line of immune defense in marine animals. Their distributions are greatly variable even in the close animal species. In this study, we used lectin staining to aid in the classification and purification of these cells for further investigation of SOD distribution among coelomocytes of H. scraba. We classified coelomocytes into four types: type 1, lymphocytes; type 2, phagocytes; type 3, spherulocytes; and type 4, giant cells. Among four lectins used, Con A appeared to give a broad reactivity against most coelomocytes, except for giant cells. In addition, phagocytes usually engaged the highest fluorescent intensity with most lectins, with the exception of PNA, for which spherulocytes possessed the highest fluorescent intensity. Using FACS for fraction collection, it was found that F1 fraction contained the purest phagocyte population (&gt; 95%), which was highly reactive with anti- superoxide dismutase (SOD) as revealed by immunoblotting and immunofluorescen...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15068666"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15068666"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15068666; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15068666]").text(description); $(".js-view-count[data-work-id=15068666]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15068666; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15068666']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15068666, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15068666]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15068666,"title":"Lectin-Based Profiling of Coelomocytes in Holothuria scabra and Expression of Superoxide Dismutase in Purified Coelomocytes","translated_title":"","metadata":{"abstract":"Coelomocytes are the first line of immune defense in marine animals. Their distributions are greatly variable even in the close animal species. In this study, we used lectin staining to aid in the classification and purification of these cells for further investigation of SOD distribution among coelomocytes of H. scraba. We classified coelomocytes into four types: type 1, lymphocytes; type 2, phagocytes; type 3, spherulocytes; and type 4, giant cells. Among four lectins used, Con A appeared to give a broad reactivity against most coelomocytes, except for giant cells. In addition, phagocytes usually engaged the highest fluorescent intensity with most lectins, with the exception of PNA, for which spherulocytes possessed the highest fluorescent intensity. Using FACS for fraction collection, it was found that F1 fraction contained the purest phagocyte population (\u0026gt; 95%), which was highly reactive with anti- superoxide dismutase (SOD) as revealed by immunoblotting and immunofluorescen...","publication_date":{"day":null,"month":null,"year":2015,"errors":{}},"publication_name":"Zoological science"},"translated_abstract":"Coelomocytes are the first line of immune defense in marine animals. Their distributions are greatly variable even in the close animal species. In this study, we used lectin staining to aid in the classification and purification of these cells for further investigation of SOD distribution among coelomocytes of H. scraba. We classified coelomocytes into four types: type 1, lymphocytes; type 2, phagocytes; type 3, spherulocytes; and type 4, giant cells. Among four lectins used, Con A appeared to give a broad reactivity against most coelomocytes, except for giant cells. 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TNF-α upregulates intercellular adhesion molecule (ICAM)-1 expression on the RPE, which allows lymphocyte function-associated antigen-1 (LFA-1) to bind on leukocytes that contribute to leukocyte adhesion at sites of inflammation. Vascular endothelial growth factor (VEGF)-A(165)b is generated by alternative splicing of VEGF-A in the terminal exon, exon 8. VEGF-A(165)b is cytoprotective and antiangiogenic, but its effects on inflammation have not yet been elucidated. Therefore, we tested the hypothesis that VEGF-A(165)b regulates TNF-α-induced ICAM-1 expression and monocyte adhesion in RPE cells. Primary RPE cells were pretreated with TNF-α alone, VEGF-A(165)b alone, VEGF-A(165)b with anti-VEGF-A(165)b, or the VEGFR-2 inhibitor ZM323881 before exposure to TNF-α for 24 h. Western blotting and monocyte adhesion assays w...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8e7320df460c0aa49c67b02029966500" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431577,"asset_id":15211924,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431577/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&st=MTczMjc5ODg1NSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211924"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211924"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211924; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211924]").text(description); $(".js-view-count[data-work-id=15211924]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211924; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211924']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211924, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8e7320df460c0aa49c67b02029966500" } } $('.js-work-strip[data-work-id=15211924]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211924,"title":"TNF-α-induced ICAM-1 expression and monocyte adhesion in human RPE cells is mediated in part through autocrine VEGF stimulation","translated_title":"","metadata":{"abstract":"Local inflammation at the RPE cell layer is associated with inflammatory cell migration and secretion of proinflammatory cytokines such as tumor necrosis factor (TNF)-α. 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Western blotting and monocyte adhesion assays w...","publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"Molecular vision"},"translated_abstract":"Local inflammation at the RPE cell layer is associated with inflammatory cell migration and secretion of proinflammatory cytokines such as tumor necrosis factor (TNF)-α. TNF-α upregulates intercellular adhesion molecule (ICAM)-1 expression on the RPE, which allows lymphocyte function-associated antigen-1 (LFA-1) to bind on leukocytes that contribute to leukocyte adhesion at sites of inflammation. Vascular endothelial growth factor (VEGF)-A(165)b is generated by alternative splicing of VEGF-A in the terminal exon, exon 8. VEGF-A(165)b is cytoprotective and antiangiogenic, but its effects on inflammation have not yet been elucidated. Therefore, we tested the hypothesis that VEGF-A(165)b regulates TNF-α-induced ICAM-1 expression and monocyte adhesion in RPE cells. Primary RPE cells were pretreated with TNF-α alone, VEGF-A(165)b alone, VEGF-A(165)b with anti-VEGF-A(165)b, or the VEGFR-2 inhibitor ZM323881 before exposure to TNF-α for 24 h. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211923"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211923/Diarylheptanoid_7_3_4_dihydroxyphenyl_5_hydroxy_1_phenyl_1E_1_heptene_from_Curcuma_comosa_Roxb_protects_retinal_pigment_epithelial_cells_against_oxidative_stress_induced_cell_death"><img alt="Research paper thumbnail of Diarylheptanoid 7-(3,4 dihydroxyphenyl)-5-hydroxy-1-phenyl-(1E)-1-heptene from Curcuma comosa Roxb. protects retinal pigment epithelial cells against oxidative stress-induced cell death" class="work-thumbnail" src="https://attachments.academia-assets.com/43431571/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211923/Diarylheptanoid_7_3_4_dihydroxyphenyl_5_hydroxy_1_phenyl_1E_1_heptene_from_Curcuma_comosa_Roxb_protects_retinal_pigment_epithelial_cells_against_oxidative_stress_induced_cell_death">Diarylheptanoid 7-(3,4 dihydroxyphenyl)-5-hydroxy-1-phenyl-(1E)-1-heptene from Curcuma comosa Roxb. protects retinal pigment epithelial cells against oxidative stress-induced cell death</a></div><div class="wp-workCard_item"><span>Toxicology in Vitro</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5bf22dd981615dcab264c02cff58a4db" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431571,"asset_id":15211923,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431571/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&st=MTczMjc5ODg1NSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211923"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211923"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211923; 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Anti-oxidants provide a natural defense against retinal cell damage. The present study was designed to evaluate the potential anti-oxidant activity and protective effect of two diarylheptanoids isolated from a medicinal herb Curcuma comosa; 7-(3,4 dihydroxyphenyl)-5-hydroxy-1-phenyl-(1E)-1-heptene (compound A), and 1,7-diphenyl-4(E),6(E)-heptadien-3-ol (compound B) against oxidative stress (H 2 O 2 )-induced human retinal pigment epithelial (APRE-19) cell death. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay indicated that the anti-oxidant activity (IC 50 ) of compound A was similar to that of vitamin C. Pre-treatment of ARPE-19 cells with 20 lM compound A for 4 h afforded greater protection against the insult from 500 lM H 2 O 2 , compared to a similar protection period for compound B. Compound A lowered H 2 O 2induced lipid peroxidation, malondialdehyde formation and intracellular reactive oxygen species. Furthermore, compound A ameliorated the H 2 O 2 -induced decrease in anti-oxidant enzyme activities and subsequent apoptotic cell death in ARPE-19 cells in a dose and time-dependent manner. These results suggest that compound A protects ARPE-19 cells against oxidative stress, in part, by enhancing several anti-oxidant defense mechanisms. Therefore, compound A may have therapeutic potential for diseases associated with oxidative stress, particularly degenerative retinal diseases.","publication_date":{"day":null,"month":null,"year":2011,"errors":{}},"publication_name":"Toxicology in Vitro","grobid_abstract_attachment_id":43431571},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211923/Diarylheptanoid_7_3_4_dihydroxyphenyl_5_hydroxy_1_phenyl_1E_1_heptene_from_Curcuma_comosa_Roxb_protects_retinal_pigment_epithelial_cells_against_oxidative_stress_induced_cell_death","translated_internal_url":"","created_at":"2015-08-26T18:26:35.072-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124446,"work_id":15211923,"tagging_user_id":34271684,"tagged_user_id":4453629,"co_author_invite_id":null,"email":"p***e@gmail.com","display_order":0,"name":"Pawinee 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href="https://www.academia.edu/15211922/In_vitro_protection_against_hydrogen_peroxide_induced_oxidative_stress_and_cell_death_in_ARPE_19_cells_by_Curcumin"><img alt="Research paper thumbnail of In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211922/In_vitro_protection_against_hydrogen_peroxide_induced_oxidative_stress_and_cell_death_in_ARPE_19_cells_by_Curcumin">In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin</a></div><div class="wp-workCard_item"><span>Planta Medica</span><span>, 2010</span></div><div class="wp-workCard_item 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})(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15211922]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211922,"title":"In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Planta Medica"},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211922/In_vitro_protection_against_hydrogen_peroxide_induced_oxidative_stress_and_cell_death_in_ARPE_19_cells_by_Curcumin","translated_internal_url":"","created_at":"2015-08-26T18:26:34.950-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124453,"work_id":15211922,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":1153117,"email":"y***t@mahidol.ac.th","display_order":0,"name":"Y. Kitiyanant","title":"In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin"}],"downloadable_attachments":[],"slug":"In_vitro_protection_against_hydrogen_peroxide_induced_oxidative_stress_and_cell_death_in_ARPE_19_cells_by_Curcumin","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[],"research_interests":[{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine"},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology"},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211921"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211921/Cloning_and_characterization_of_a_caspase_gene_from_black_tiger_shrimp_Penaeus_monodon_infected_with_white_spot_syndrome_virus_WSSV_"><img alt="Research paper thumbnail of Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus monodon)-infected with white spot syndrome virus (WSSV)" class="work-thumbnail" src="https://attachments.academia-assets.com/43431603/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211921/Cloning_and_characterization_of_a_caspase_gene_from_black_tiger_shrimp_Penaeus_monodon_infected_with_white_spot_syndrome_virus_WSSV_">Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus monodon)-infected with white spot syndrome virus (WSSV)</a></div><div class="wp-workCard_item"><span>Journal of Biotechnology</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="961a8d20a17362a48b83e87b09e26003" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431603,"asset_id":15211921,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431603/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211921"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211921"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211921; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211921]").text(description); $(".js-view-count[data-work-id=15211921]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211921; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211921']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211921, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "961a8d20a17362a48b83e87b09e26003" } } $('.js-work-strip[data-work-id=15211921]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211921,"title":"Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus monodon)-infected with white spot syndrome virus (WSSV)","translated_title":"","metadata":{"grobid_abstract":"A black tiger shrimp (Penaeus monodon) caspase cDNA homologue (PmCasp) has been identified from a hemocyte library using a previously identified caspase homologue from the banana shrimp (Penaeus merguiensis) as a probe. The full-length PmCasp was 1202 bp with a 954 bp open reading frame, encoding 317 amino acids. The deduced protein contained a potential active site (QACRG pentapeptide) conserved in most caspases. It had 83% identity with caspase of P. merguiensis and 30% identity with drICE protein of Drosophila melanogaster, and it exhibited caspase-3 activity in vitro. PmCasp was cloned and expressed in Escherichia coli and a rabbit polyclonal antiserum was produced. In Western blots, the antiserum reacted with purified recombinant PmCasp and with lysates of E. coli containing the expressed plasmid. In crude protein extracts from normal shrimp, the antiserum reacted with 36 and 26 kDa bands likely to correspond to inactive pro-caspase and its proteolytic intermediate form, respectively. PmCasp expression was measured in normal shrimp and in white spot syndrome virus (WSSV)-infected shrimp at 24 and 48 h post-injection (p.i.) by semi-quantitative RT-PCR, Western blot analysis, and immunohistochemistry. Semi-quantitative RT-PCR analysis revealed up-regulation of PmCasp at 48 h p.i. and expression remained high up to the moribund state. These results were supported by Western blot analysis showing increased PmCasp protein levels at 24 and 48 h p.i. when compared to normal control shrimp. Immunohistochemical analysis of gills from the WSSV-infected shrimp revealed immunoreactivity localized in the cytoplasm of both normal and apparently apoptotic cells. In summary, a caspase-3 like gene is conserved in P. monodon and is up-regulated after WSSV infection.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Journal of Biotechnology","grobid_abstract_attachment_id":43431603},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211921/Cloning_and_characterization_of_a_caspase_gene_from_black_tiger_shrimp_Penaeus_monodon_infected_with_white_spot_syndrome_virus_WSSV_","translated_internal_url":"","created_at":"2015-08-26T18:26:34.821-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124454,"work_id":15211921,"tagging_user_id":34271684,"tagged_user_id":13877169,"co_author_invite_id":null,"email":"s***0@gmail.com","display_order":0,"name":"Saengchan Senapin","title":"Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus 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blot","url":"https://www.academia.edu/Documents/in/Western_blot"},{"id":462837,"name":"Gills","url":"https://www.academia.edu/Documents/in/Gills"},{"id":585573,"name":"Amino Acid Profile","url":"https://www.academia.edu/Documents/in/Amino_Acid_Profile"},{"id":645803,"name":"Crude Protein","url":"https://www.academia.edu/Documents/in/Crude_Protein"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":844925,"name":"Open Reading Frame","url":"https://www.academia.edu/Documents/in/Open_Reading_Frame"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1181139,"name":"White Spot Syndrome Virus","url":"https://www.academia.edu/Documents/in/White_Spot_Syndrome_Virus"},{"id":1435468,"name":"Penaeidae","url":"https://www.academia.edu/Documents/in/Penaeidae"},{"id":1457054,"name":"Protein Transport","url":"https://www.academia.edu/Documents/in/Protein_Transport"},{"id":1556410,"name":"Full Length Movies","url":"https://www.academia.edu/Documents/in/Full_Length_Movies"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211920"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211920/Culture_and_development_of_the_polychaete_Perinereis_cf_nuntia"><img alt="Research paper thumbnail of Culture and development of the polychaete Perinereis cf. nuntia" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211920/Culture_and_development_of_the_polychaete_Perinereis_cf_nuntia">Culture and development of the polychaete Perinereis cf. nuntia</a></div><div class="wp-workCard_item"><span>Invertebrate Reproduction & Development</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT The polychaete Perinereis cf. nuntia, a tropical species endemic in Thailand, was cultur...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT The polychaete Perinereis cf. nuntia, a tropical species endemic in Thailand, was cultured in captivity as follows: eggs and sperm from sexually mature (epitokous) P. cf. nuntia, were artificially fertilized, and settled into a sand bed about 30 cm deep at the nectochaete stage. The sand beds were supplied with seawater at 30-ppt salinity and the nectochaetes reared for five months after which time some adults were becoming epitokes. The culture method yielded 3–4 kg polychaetes at an atokous stage per m of culture area. Because the polychaetes were to be used to feed shrimp broodstock, samples of the worms were screened for the presence of white-spot syndrome and yellow-head viruses using polymerase chain reaction (PCR) methods prior to introduction to the system as founders and at monthly intervals during their culture. Morphological details of P. cf. nuntia from fertilization to nectochaete stage are described using light microscopy, transmission (TEM) and scanning electron microscopy (SEM). The egg is surrounded by a chorion layer, TEM reveals that the microvillous tip vesicles are putative sites for the binding of the sperm to the egg surface. TEM studies of the cortical reaction show that the cortical alveoli contain a fibrous substance which, after fertilization and membrane fusion, passes into the perivitelline space to form part of the fertilization membrane, as well as being secreted from the egg surface to form a jelly layer surrounding the egg.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211920"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211920"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211920; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211920]").text(description); $(".js-view-count[data-work-id=15211920]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211920; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211920']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211920, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15211920]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211920,"title":"Culture and development of the polychaete Perinereis cf. nuntia","translated_title":"","metadata":{"abstract":"ABSTRACT The polychaete Perinereis cf. nuntia, a tropical species endemic in Thailand, was cultured in captivity as follows: eggs and sperm from sexually mature (epitokous) P. cf. nuntia, were artificially fertilized, and settled into a sand bed about 30 cm deep at the nectochaete stage. The sand beds were supplied with seawater at 30-ppt salinity and the nectochaetes reared for five months after which time some adults were becoming epitokes. The culture method yielded 3–4 kg polychaetes at an atokous stage per m of culture area. Because the polychaetes were to be used to feed shrimp broodstock, samples of the worms were screened for the presence of white-spot syndrome and yellow-head viruses using polymerase chain reaction (PCR) methods prior to introduction to the system as founders and at monthly intervals during their culture. Morphological details of P. cf. nuntia from fertilization to nectochaete stage are described using light microscopy, transmission (TEM) and scanning electron microscopy (SEM). The egg is surrounded by a chorion layer, TEM reveals that the microvillous tip vesicles are putative sites for the binding of the sperm to the egg surface. TEM studies of the cortical reaction show that the cortical alveoli contain a fibrous substance which, after fertilization and membrane fusion, passes into the perivitelline space to form part of the fertilization membrane, as well as being secreted from the egg surface to form a jelly layer surrounding the egg.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Invertebrate Reproduction \u0026 Development"},"translated_abstract":"ABSTRACT The polychaete Perinereis cf. nuntia, a tropical species endemic in Thailand, was cultured in captivity as follows: eggs and sperm from sexually mature (epitokous) P. cf. nuntia, were artificially fertilized, and settled into a sand bed about 30 cm deep at the nectochaete stage. The sand beds were supplied with seawater at 30-ppt salinity and the nectochaetes reared for five months after which time some adults were becoming epitokes. The culture method yielded 3–4 kg polychaetes at an atokous stage per m of culture area. Because the polychaetes were to be used to feed shrimp broodstock, samples of the worms were screened for the presence of white-spot syndrome and yellow-head viruses using polymerase chain reaction (PCR) methods prior to introduction to the system as founders and at monthly intervals during their culture. Morphological details of P. cf. nuntia from fertilization to nectochaete stage are described using light microscopy, transmission (TEM) and scanning electron microscopy (SEM). The egg is surrounded by a chorion layer, TEM reveals that the microvillous tip vesicles are putative sites for the binding of the sperm to the egg surface. TEM studies of the cortical reaction show that the cortical alveoli contain a fibrous substance which, after fertilization and membrane fusion, passes into the perivitelline space to form part of the fertilization membrane, as well as being secreted from the egg surface to form a jelly layer surrounding the egg.","internal_url":"https://www.academia.edu/15211920/Culture_and_development_of_the_polychaete_Perinereis_cf_nuntia","translated_internal_url":"","created_at":"2015-08-26T18:26:34.691-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124452,"work_id":15211920,"tagging_user_id":34271684,"tagged_user_id":35784640,"co_author_invite_id":1063008,"email":"p***b@mahidol.ac.th","display_order":0,"name":"Prasert Sobhon","title":"Culture and development of the polychaete Perinereis cf. nuntia"}],"downloadable_attachments":[],"slug":"Culture_and_development_of_the_polychaete_Perinereis_cf_nuntia","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":10655,"name":"Scanning Electron Microscopy","url":"https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction"},{"id":122189,"name":"Membrane Fusion","url":"https://www.academia.edu/Documents/in/Membrane_Fusion"},{"id":541736,"name":"Sexual maturity","url":"https://www.academia.edu/Documents/in/Sexual_maturity"},{"id":885893,"name":"Light microscopy","url":"https://www.academia.edu/Documents/in/Light_microscopy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15068663"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15068663/Solvent_extracts_of_the_red_seaweed_Gracilaria_fisheri_prevent_Vibrio_harveyi_infections_in_the_black_tiger_shrimp_Penaeus_monodon"><img alt="Research paper thumbnail of Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15068663/Solvent_extracts_of_the_red_seaweed_Gracilaria_fisheri_prevent_Vibrio_harveyi_infections_in_the_black_tiger_shrimp_Penaeus_monodon">Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://mahidol.academia.edu/SomlukAsuvapongpatana">Somluk Asuvapongpatana</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BoonsirmWithyachumnarnkul">Boonsirm Withyachumnarnkul</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a></span></div><div class="wp-workCard_item"><span>Fish & Shellfish Immunology</span><span>, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shrimp. Since compounds from seaweed have been reported to have anti-bacterial and immunostimulant activity, this study was conducted to determine whether solvent extracts from the red seaweed Gracilaria fisheri might be a possible alternative for prevention and treatment of shrimp vibriosis caused by Vibrio harveyi. Seaweed extracts prepared using ethanol, methanol, chloroform and hexane were evaluated for anti-V. harveyi activity by the disc-diffusion method. The ethanol, methanol and chloroform extracts showed activity against a virulent strain of V. harveyi with potency (minimal inhibitory concentrations in the range of 90-190 μg ml(-1)) equivalent to the antibiotic norfloxacin. The ethanol extract was not toxic to the brine shrimp Artemia salina when it was fed to them for enrichment prior to their use, in turn, as feed for postlarvae of Penaeus monodon. Postlarvae fed with these enriched Artemia gave significantly lower mortality than control postlarvae after challenge with V. harveyi. In addition, P. monodon juveniles injected with the ethanol extract showed a significant increase in the total number of haemocytes and an increased proportion of semi-granulocytes and granulocytes when compared to control shrimp. The activities of phenoloxidase and superoxide dismutase were also increased, with an accompanying increase in superoxide anion production. When these juvenile shrimp were challenged with V. harveyi, mortality was markedly reduced compared to that of control shrimp. The results indicated that ethanol extracts of G. fisheri had immunostimulant and antimicrobial activity that could protect P. monodon against V. harveyi.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15068663"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15068663"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15068663; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15068663]").text(description); $(".js-view-count[data-work-id=15068663]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15068663; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15068663']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15068663, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15068663]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15068663,"title":"Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon","translated_title":"","metadata":{"abstract":"Vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shrimp. Since compounds from seaweed have been reported to have anti-bacterial and immunostimulant activity, this study was conducted to determine whether solvent extracts from the red seaweed Gracilaria fisheri might be a possible alternative for prevention and treatment of shrimp vibriosis caused by Vibrio harveyi. Seaweed extracts prepared using ethanol, methanol, chloroform and hexane were evaluated for anti-V. harveyi activity by the disc-diffusion method. The ethanol, methanol and chloroform extracts showed activity against a virulent strain of V. harveyi with potency (minimal inhibitory concentrations in the range of 90-190 μg ml(-1)) equivalent to the antibiotic norfloxacin. The ethanol extract was not toxic to the brine shrimp Artemia salina when it was fed to them for enrichment prior to their use, in turn, as feed for postlarvae of Penaeus monodon. Postlarvae fed with these enriched Artemia gave significantly lower mortality than control postlarvae after challenge with V. harveyi. In addition, P. monodon juveniles injected with the ethanol extract showed a significant increase in the total number of haemocytes and an increased proportion of semi-granulocytes and granulocytes when compared to control shrimp. The activities of phenoloxidase and superoxide dismutase were also increased, with an accompanying increase in superoxide anion production. When these juvenile shrimp were challenged with V. harveyi, mortality was markedly reduced compared to that of control shrimp. The results indicated that ethanol extracts of G. fisheri had immunostimulant and antimicrobial activity that could protect P. monodon against V. harveyi.","publication_date":{"day":null,"month":null,"year":2011,"errors":{}},"publication_name":"Fish \u0026 Shellfish Immunology"},"translated_abstract":"Vibriosis is a common bacterial disease that can cause high mortality and morbidity in farmed shrimp. Since compounds from seaweed have been reported to have anti-bacterial and immunostimulant activity, this study was conducted to determine whether solvent extracts from the red seaweed Gracilaria fisheri might be a possible alternative for prevention and treatment of shrimp vibriosis caused by Vibrio harveyi. Seaweed extracts prepared using ethanol, methanol, chloroform and hexane were evaluated for anti-V. harveyi activity by the disc-diffusion method. The ethanol, methanol and chloroform extracts showed activity against a virulent strain of V. harveyi with potency (minimal inhibitory concentrations in the range of 90-190 μg ml(-1)) equivalent to the antibiotic norfloxacin. The ethanol extract was not toxic to the brine shrimp Artemia salina when it was fed to them for enrichment prior to their use, in turn, as feed for postlarvae of Penaeus monodon. Postlarvae fed with these enriched Artemia gave significantly lower mortality than control postlarvae after challenge with V. harveyi. In addition, P. monodon juveniles injected with the ethanol extract showed a significant increase in the total number of haemocytes and an increased proportion of semi-granulocytes and granulocytes when compared to control shrimp. The activities of phenoloxidase and superoxide dismutase were also increased, with an accompanying increase in superoxide anion production. When these juvenile shrimp were challenged with V. harveyi, mortality was markedly reduced compared to that of control shrimp. The results indicated that ethanol extracts of G. fisheri had immunostimulant and antimicrobial activity that could protect P. monodon against V. harveyi.","internal_url":"https://www.academia.edu/15068663/Solvent_extracts_of_the_red_seaweed_Gracilaria_fisheri_prevent_Vibrio_harveyi_infections_in_the_black_tiger_shrimp_Penaeus_monodon","translated_internal_url":"","created_at":"2015-08-21T00:50:32.621-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34098982,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":4880142,"work_id":15068663,"tagging_user_id":34098982,"tagged_user_id":34291986,"co_author_invite_id":1108791,"email":"w***m@yahoo.com","display_order":0,"name":"Boonsirm Withyachumnarnkul","title":"Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon"},{"id":4880147,"work_id":15068663,"tagging_user_id":34098982,"tagged_user_id":34271684,"co_author_invite_id":1108792,"email":"k***n@mahidol.ac.th","display_order":4194304,"name":"Kanokpan Wongprasert","title":"Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon"},{"id":4880149,"work_id":15068663,"tagging_user_id":34098982,"tagged_user_id":null,"co_author_invite_id":1108793,"email":"k***5@gmail.com","display_order":6291456,"name":"Kulwadee Kanjana","title":"Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon"}],"downloadable_attachments":[],"slug":"Solvent_extracts_of_the_red_seaweed_Gracilaria_fisheri_prevent_Vibrio_harveyi_infections_in_the_black_tiger_shrimp_Penaeus_monodon","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34098982,"first_name":"Somluk","middle_initials":null,"last_name":"Asuvapongpatana","page_name":"SomlukAsuvapongpatana","domain_name":"mahidol","created_at":"2015-08-21T00:49:58.011-07:00","display_name":"Somluk Asuvapongpatana","url":"https://mahidol.academia.edu/SomlukAsuvapongpatana"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":8058,"name":"Artemia","url":"https://www.academia.edu/Documents/in/Artemia"},{"id":80451,"name":"Solvent Extraction","url":"https://www.academia.edu/Documents/in/Solvent_Extraction"},{"id":111007,"name":"Antimicrobial activity","url":"https://www.academia.edu/Documents/in/Antimicrobial_activity"},{"id":127957,"name":"Penaeus Monodon","url":"https://www.academia.edu/Documents/in/Penaeus_Monodon"},{"id":170652,"name":"Fisheries Sciences","url":"https://www.academia.edu/Documents/in/Fisheries_Sciences"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts"},{"id":379748,"name":"Vibrio","url":"https://www.academia.edu/Documents/in/Vibrio"},{"id":442493,"name":"Larva","url":"https://www.academia.edu/Documents/in/Larva"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":743643,"name":"Host Pathogen Interactions","url":"https://www.academia.edu/Documents/in/Host_Pathogen_Interactions"},{"id":1035964,"name":"Superoxide Anion","url":"https://www.academia.edu/Documents/in/Superoxide_Anion"},{"id":1435468,"name":"Penaeidae","url":"https://www.academia.edu/Documents/in/Penaeidae"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211919"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211919/Withering_syndrome_in_the_abalone_Haliotis_diversicolor_supertexta"><img alt="Research paper thumbnail of Withering syndrome in the abalone Haliotis diversicolor supertexta" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211919/Withering_syndrome_in_the_abalone_Haliotis_diversicolor_supertexta">Withering syndrome in the abalone Haliotis diversicolor supertexta</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://uw-us.academia.edu/CarolynFriedman">Carolyn Friedman</a></span></div><div class="wp-workCard_item"><span>Diseases of Aquatic Organisms</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic H...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic Haliotis diversicolor supertexta and the native H. asinina. Withering syndrome (WS) in abalone is caused by an infection with the Rickettsia-like organism (RLO) &amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Candidatus Xenohaliotis californiensis&amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; and has been spread to many countries globally. The present study reports the first observation of the WS-RLO agent in the small abalone, H. diversicolor supertexta in Thailand, Taiwan (ROC) and the People&amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s Republic of China (PRC). Under light microscopy, the RLO was observed as intracytoplasmic inclusions within epithelial cells lining the post-esophagus and, to a minor extent, the intestine of H. diversicolor. Under transmission electron microscopy, inclusions were characterized as colonies of rod-shaped bacteria, 200 x 1800 nm in size, within a vesicle in the cytoplasm of the infected cell. The RLO from the small abalone bound with WS-RLO-specific in situ hybridization probes and was amplified by polymerase chain reaction (PCR), using primers designed from the 16S rDNA sequence of the original WS-RLO from California, USA. The PCR product of RLO samples from both the PRC and Thailand showed extremely high identity with the California WS-RLO (100 and 99%, respectively). These data combined with the history of abalone movements for aquaculture purposes indicate that RLOs observed in Thailand, Taiwan and the PRC are the WS-RLO that originated from California.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211919"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211919"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211919; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211919]").text(description); $(".js-view-count[data-work-id=15211919]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211919; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211919']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211919, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15211919]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211919,"title":"Withering syndrome in the abalone Haliotis diversicolor supertexta","translated_title":"","metadata":{"abstract":"Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic Haliotis diversicolor supertexta and the native H. asinina. Withering syndrome (WS) in abalone is caused by an infection with the Rickettsia-like organism (RLO) \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Candidatus Xenohaliotis californiensis\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; and has been spread to many countries globally. The present study reports the first observation of the WS-RLO agent in the small abalone, H. diversicolor supertexta in Thailand, Taiwan (ROC) and the People\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s Republic of China (PRC). Under light microscopy, the RLO was observed as intracytoplasmic inclusions within epithelial cells lining the post-esophagus and, to a minor extent, the intestine of H. diversicolor. Under transmission electron microscopy, inclusions were characterized as colonies of rod-shaped bacteria, 200 x 1800 nm in size, within a vesicle in the cytoplasm of the infected cell. The RLO from the small abalone bound with WS-RLO-specific in situ hybridization probes and was amplified by polymerase chain reaction (PCR), using primers designed from the 16S rDNA sequence of the original WS-RLO from California, USA. The PCR product of RLO samples from both the PRC and Thailand showed extremely high identity with the California WS-RLO (100 and 99%, respectively). These data combined with the history of abalone movements for aquaculture purposes indicate that RLOs observed in Thailand, Taiwan and the PRC are the WS-RLO that originated from California.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Diseases of Aquatic Organisms"},"translated_abstract":"Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic Haliotis diversicolor supertexta and the native H. asinina. Withering syndrome (WS) in abalone is caused by an infection with the Rickettsia-like organism (RLO) \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Candidatus Xenohaliotis californiensis\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; and has been spread to many countries globally. The present study reports the first observation of the WS-RLO agent in the small abalone, H. diversicolor supertexta in Thailand, Taiwan (ROC) and the People\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s Republic of China (PRC). Under light microscopy, the RLO was observed as intracytoplasmic inclusions within epithelial cells lining the post-esophagus and, to a minor extent, the intestine of H. diversicolor. Under transmission electron microscopy, inclusions were characterized as colonies of rod-shaped bacteria, 200 x 1800 nm in size, within a vesicle in the cytoplasm of the infected cell. The RLO from the small abalone bound with WS-RLO-specific in situ hybridization probes and was amplified by polymerase chain reaction (PCR), using primers designed from the 16S rDNA sequence of the original WS-RLO from California, USA. The PCR product of RLO samples from both the PRC and Thailand showed extremely high identity with the California WS-RLO (100 and 99%, respectively). These data combined with the history of abalone movements for aquaculture purposes indicate that RLOs observed in Thailand, Taiwan and the PRC are the WS-RLO that originated from California.","internal_url":"https://www.academia.edu/15211919/Withering_syndrome_in_the_abalone_Haliotis_diversicolor_supertexta","translated_internal_url":"","created_at":"2015-08-26T18:26:34.484-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124447,"work_id":15211919,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":256290,"email":"c***f@u.washington.edu","display_order":0,"name":"Carolyn Friedman","title":"Withering syndrome in the abalone Haliotis diversicolor supertexta"},{"id":5124448,"work_id":15211919,"tagging_user_id":34271684,"tagged_user_id":42296411,"co_author_invite_id":256291,"email":"c***f@uw.edu","affiliation":"UW","display_order":4194304,"name":"Carolyn Friedman","title":"Withering syndrome in the abalone Haliotis diversicolor supertexta"}],"downloadable_attachments":[],"slug":"Withering_syndrome_in_the_abalone_Haliotis_diversicolor_supertexta","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[],"research_interests":[{"id":23848,"name":"Aquaculture","url":"https://www.academia.edu/Documents/in/Aquaculture"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":85919,"name":"Gastropoda","url":"https://www.academia.edu/Documents/in/Gastropoda"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":1288215,"name":"Rickettsia","url":"https://www.academia.edu/Documents/in/Rickettsia"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15068662"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15068662/Adaptation_of_the_black_tiger_shrimp_Penaeus_monodon_to_different_salinities_through_an_excretory_function_of_the_antennal_gland"><img alt="Research paper thumbnail of Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15068662/Adaptation_of_the_black_tiger_shrimp_Penaeus_monodon_to_different_salinities_through_an_excretory_function_of_the_antennal_gland">Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://mahidol.academia.edu/SomlukAsuvapongpatana">Somluk Asuvapongpatana</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BoonsirmWithyachumnarnkul">Boonsirm Withyachumnarnkul</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a></span></div><div class="wp-workCard_item"><span>Cell and Tissue Research</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinit...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular adaptation of their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans. We have investigated the morphological and biochemical alterations of the antennal glands in shrimp reared in 7, 15, or 30 ppt seawater. Drastic changes occur in animals reared under 7 ppt conditions. Ultrastructural studies of the antennal gland in shrimps reared in 7 ppt seawater have revealed that podocytic cells in the coelomosacs ramify with more cytoplasmic processes forming the filtration slits, and that the tubular labyrinth cells possess more mitochondria in their basal striation and a wider tubular lumen than those found in the other groups. Many apical cytoplasmic blebs from labyrinth cells have also been seen in the lumen of the labyrinths under 7 ppt conditions, a feature that is not as prominent under the other conditions. The expression and activity of the Na(+)/K(+)-ATPase in the antennal gland are also correlated with the surrounding environment: the lower the salinity, the higher the expression and activity of the enzyme. Immunohistochemistry results have demonstrated the highest staining intensity in the labyrinth cells of shrimps reared under 7 ppt conditions. Our findings thus suggest that one of the adaptation mechanisms of this shrimp to the surrounding salinity is the regulation of Na(+)/K(+)-ATPase expression in the antennal gland, in conjunction with subcellular changes in its excretory cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15068662"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15068662"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15068662; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15068662]").text(description); $(".js-view-count[data-work-id=15068662]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15068662; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15068662']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15068662, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=15068662]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15068662,"title":"Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland","translated_title":"","metadata":{"abstract":"Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular adaptation of their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans. We have investigated the morphological and biochemical alterations of the antennal glands in shrimp reared in 7, 15, or 30 ppt seawater. Drastic changes occur in animals reared under 7 ppt conditions. Ultrastructural studies of the antennal gland in shrimps reared in 7 ppt seawater have revealed that podocytic cells in the coelomosacs ramify with more cytoplasmic processes forming the filtration slits, and that the tubular labyrinth cells possess more mitochondria in their basal striation and a wider tubular lumen than those found in the other groups. Many apical cytoplasmic blebs from labyrinth cells have also been seen in the lumen of the labyrinths under 7 ppt conditions, a feature that is not as prominent under the other conditions. The expression and activity of the Na(+)/K(+)-ATPase in the antennal gland are also correlated with the surrounding environment: the lower the salinity, the higher the expression and activity of the enzyme. Immunohistochemistry results have demonstrated the highest staining intensity in the labyrinth cells of shrimps reared under 7 ppt conditions. Our findings thus suggest that one of the adaptation mechanisms of this shrimp to the surrounding salinity is the regulation of Na(+)/K(+)-ATPase expression in the antennal gland, in conjunction with subcellular changes in its excretory cells.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Cell and Tissue Research"},"translated_abstract":"Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular adaptation of their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans. We have investigated the morphological and biochemical alterations of the antennal glands in shrimp reared in 7, 15, or 30 ppt seawater. Drastic changes occur in animals reared under 7 ppt conditions. Ultrastructural studies of the antennal gland in shrimps reared in 7 ppt seawater have revealed that podocytic cells in the coelomosacs ramify with more cytoplasmic processes forming the filtration slits, and that the tubular labyrinth cells possess more mitochondria in their basal striation and a wider tubular lumen than those found in the other groups. Many apical cytoplasmic blebs from labyrinth cells have also been seen in the lumen of the labyrinths under 7 ppt conditions, a feature that is not as prominent under the other conditions. The expression and activity of the Na(+)/K(+)-ATPase in the antennal gland are also correlated with the surrounding environment: the lower the salinity, the higher the expression and activity of the enzyme. Immunohistochemistry results have demonstrated the highest staining intensity in the labyrinth cells of shrimps reared under 7 ppt conditions. Our findings thus suggest that one of the adaptation mechanisms of this shrimp to the surrounding salinity is the regulation of Na(+)/K(+)-ATPase expression in the antennal gland, in conjunction with subcellular changes in its excretory cells.","internal_url":"https://www.academia.edu/15068662/Adaptation_of_the_black_tiger_shrimp_Penaeus_monodon_to_different_salinities_through_an_excretory_function_of_the_antennal_gland","translated_internal_url":"","created_at":"2015-08-21T00:50:32.520-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34098982,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":4880135,"work_id":15068662,"tagging_user_id":34098982,"tagged_user_id":null,"co_author_invite_id":1089797,"email":"s***y@mahidol.ac.th","display_order":0,"name":"Wattana Weerachatyanukul","title":"Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland"},{"id":4880143,"work_id":15068662,"tagging_user_id":34098982,"tagged_user_id":34291986,"co_author_invite_id":1108791,"email":"w***m@yahoo.com","display_order":4194304,"name":"Boonsirm Withyachumnarnkul","title":"Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland"},{"id":4880148,"work_id":15068662,"tagging_user_id":34098982,"tagged_user_id":34271684,"co_author_invite_id":1108792,"email":"k***n@mahidol.ac.th","display_order":6291456,"name":"Kanokpan Wongprasert","title":"Adaptation of the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function of the antennal gland"}],"downloadable_attachments":[],"slug":"Adaptation_of_the_black_tiger_shrimp_Penaeus_monodon_to_different_salinities_through_an_excretory_function_of_the_antennal_gland","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":34098982,"first_name":"Somluk","middle_initials":null,"last_name":"Asuvapongpatana","page_name":"SomlukAsuvapongpatana","domain_name":"mahidol","created_at":"2015-08-21T00:49:58.011-07:00","display_name":"Somluk Asuvapongpatana","url":"https://mahidol.academia.edu/SomlukAsuvapongpatana"},"attachments":[],"research_interests":[{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry"},{"id":82107,"name":"Salinity","url":"https://www.academia.edu/Documents/in/Salinity"},{"id":127957,"name":"Penaeus Monodon","url":"https://www.academia.edu/Documents/in/Penaeus_Monodon"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme"},{"id":1435468,"name":"Penaeidae","url":"https://www.academia.edu/Documents/in/Penaeidae"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211918"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211918/Development_of_two_microsatellite_multiplex_systems_for_black_tiger_shrimp_Penaeus_monodon_and_its_application_in_genetic_diversity_study_for_two_populations"><img alt="Research paper thumbnail of Development of two microsatellite multiplex systems for black tiger shrimp Penaeus monodon and its application in genetic diversity study for two populations" class="work-thumbnail" src="https://attachments.academia-assets.com/43431597/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211918/Development_of_two_microsatellite_multiplex_systems_for_black_tiger_shrimp_Penaeus_monodon_and_its_application_in_genetic_diversity_study_for_two_populations">Development of two microsatellite multiplex systems for black tiger shrimp Penaeus monodon and its application in genetic diversity study for two populations</a></div><div class="wp-workCard_item"><span>Aquaculture</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3d389a49559718f33ec797a0a0f40af6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431597,"asset_id":15211918,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431597/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15211918"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15211918"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15211918; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15211918]").text(description); $(".js-view-count[data-work-id=15211918]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15211918; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15211918']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15211918, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3d389a49559718f33ec797a0a0f40af6" } } $('.js-work-strip[data-work-id=15211918]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15211918,"title":"Development of two microsatellite multiplex systems for black tiger shrimp Penaeus monodon and its application in genetic diversity study for two populations","translated_title":"","metadata":{"grobid_abstract":"Despite large numbers of putative microsatellites currently listed in databases for Penaeus monodon, there are no publications on assessing these markers for multiplexed high throughput systems either for fingerprinting or population genetics study purposes in P. monodon. Accordingly, we started our investigation on the development of high throughput systems for P. monodon. Ninety publicly-available P. monodon microsatellite sequences were initially screened for suitability. They were assessed for the presence of tri-or tetra-nucleotide repeats, repeat number and type, suitability of flanking sequences for primer design and estimated size of product (100 to 350 bp). Nineteen sequences were chosen for preliminary assessment on a panel of 15 animals. Of the 19 tested, only 12 were suitable for further investigation. Therefore a 2-step enrichment library approach was adopted to develop additional microsatellites. Of 42 new unique microsatellite sequences obtained, eight sequences were assessed and seven showed polymorphism. Together, these 19 markers were examined further for their ease of amplification and reliability of allele calling for inclusion in high throughput systems. Thirteen polymorphic markers were incorporated into two multiplex systems (six and seven markers, respectively). These multiplexed systems were then used to evaluate the genetic diversity between two populations of P. monodon, one from the East Coast of Australia and a single pond containing farmed animals from Thailand. There were significant differences between the two populations. Three markers in system 1 showed Hardy-Weinberg disequilibrium in both populations, indicating their unsuitability as high throughput system markers. Using two systems and the UPGMA clustering methods revealed the existence of sub-populations within the Australian wild population. The results indicate the usefulness of the two multiplexed microsatellite systems in genetic diversity studies. Crown","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Aquaculture","grobid_abstract_attachment_id":43431597},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211918/Development_of_two_microsatellite_multiplex_systems_for_black_tiger_shrimp_Penaeus_monodon_and_its_application_in_genetic_diversity_study_for_two_populations","translated_internal_url":"","created_at":"2015-08-26T18:26:33.753-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124445,"work_id":15211918,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":1004784,"email":"j***s@csiro.au","display_order":0,"name":"Jennifer Meadows","title":"Development of two microsatellite multiplex systems for black tiger shrimp Penaeus monodon and its application in genetic diversity study for two 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15211917"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15211917/Retinopathy_in_stunted_black_tiger_shrimp_Penaeus_monodon_and_possible_association_with_Laem_Singh_virus_LSNV_"><img alt="Research paper thumbnail of Retinopathy in stunted black tiger shrimp Penaeus monodon and possible association with Laem-Singh virus (LSNV)" class="work-thumbnail" src="https://attachments.academia-assets.com/43431585/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15211917/Retinopathy_in_stunted_black_tiger_shrimp_Penaeus_monodon_and_possible_association_with_Laem_Singh_virus_LSNV_">Retinopathy in stunted black tiger shrimp Penaeus monodon and possible association with Laem-Singh virus (LSNV)</a></div><div class="wp-workCard_item"><span>Aquaculture</span><span>, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="086bb30218c78ff490c88f1b629a9c85" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43431585,"asset_id":15211917,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43431585/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" 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Shrimp from these ponds often show the presence Laem-Singh virus (LSNV) in the lymphoid organ, heart and other tissues. We compared various tissues of abnormally small and normal size (large) P. monodon from 3 MSGS ponds and from 2 reference ponds with normal growth (control ponds) both histologically and for the presence of LSNV. Small and large shrimp from the MSGS ponds were positive for LSNV by reverse transcriptase polymerase chain reaction (RT-PCR) tests while shrimp from one normal-growth pond were positive and from another negative. The LSNV infections were confirmed by transmission electron microscopy (TEM) and in situ hybridization (ISH). The only striking difference between the small and large LSNV-positive shrimp was retinopathy exclusively in the small shrimp from the MSGS ponds. Retinopathy comprised abnormally enlarged haemolymphatic vessels, haemocytic infiltration and rupture of the membrane that separated the fasciculated zone from the overlying row of retinular cells. By TEM and ISH, LSNV was detected in the fasciculated zone and in onion bodies of the organ of Bellonci in the small shrimp from the MSGS ponds, but not in those tissues and cells of the large shrimp from the MSGS pond or from the normal-growth ponds, whether LSNV-positive or not. The results suggested that retinopathy associated with LSNV may be linked causally to stunting of P. monodon in MSGS ponds.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Aquaculture","grobid_abstract_attachment_id":43431585},"translated_abstract":null,"internal_url":"https://www.academia.edu/15211917/Retinopathy_in_stunted_black_tiger_shrimp_Penaeus_monodon_and_possible_association_with_Laem_Singh_virus_LSNV_","translated_internal_url":"","created_at":"2015-08-26T18:26:33.041-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34271684,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":5124451,"work_id":15211917,"tagging_user_id":34271684,"tagged_user_id":null,"co_author_invite_id":1153116,"email":"s***w@gmail.com","display_order":0,"name":"Waraporn Sakaew","title":"Retinopathy in stunted black tiger shrimp Penaeus monodon and possible association with Laem-Singh virus (LSNV)"}],"downloadable_attachments":[{"id":43431585,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/43431585/thumbnails/1.jpg","file_name":"j.aquaculture.2008.07.040.pdf20160306-22960-1eui2x6","download_url":"https://www.academia.edu/attachments/43431585/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Retinopathy_in_stunted_black_tiger_shrim.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/43431585/j.aquaculture.2008.07.040-libre.pdf20160306-22960-1eui2x6?1457298651=\u0026response-content-disposition=attachment%3B+filename%3DRetinopathy_in_stunted_black_tiger_shrim.pdf\u0026Expires=1732802456\u0026Signature=PL-BajW-RadVJ9fQus80y1E6J542Uerx9ctLaLv19PyOIGe8LpvgFK84aYAA4GKdXBLtav-RdE9LgY5aEXWOq-2Wz6c2atX9FSmP3XkWhl-ockWqHnNwZMtj7qDmNLI-9SG9j9s5NCauPzIToEavUzBiY5u49cEUpIObHIEtMt74t67gMMn2OBzyUU1yG4x1ItOvI8xfXV3P0OzvtLQ9lE7sngifPN2OSeRsxJRRVzQGjfKcvpeZ-MAlvX6hCMi7NUQbh4-AKgxL5KSy3XK2kVTqaU-FeYKttyRqiO-Fz6yz7C513hTV3xZWT44FW9sKlP2Prvno3sBtoinZODHirA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Retinopathy_in_stunted_black_tiger_shrimp_Penaeus_monodon_and_possible_association_with_Laem_Singh_virus_LSNV_","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":34271684,"first_name":"Kanokpan","middle_initials":null,"last_name":"Wongprasert","page_name":"KanokpanWongprasert","domain_name":"independent","created_at":"2015-08-26T18:25:39.426-07:00","display_name":"Kanokpan Wongprasert","url":"https://independent.academia.edu/KanokpanWongprasert"},"attachments":[{"id":43431585,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/43431585/thumbnails/1.jpg","file_name":"j.aquaculture.2008.07.040.pdf20160306-22960-1eui2x6","download_url":"https://www.academia.edu/attachments/43431585/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Retinopathy_in_stunted_black_tiger_shrim.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/43431585/j.aquaculture.2008.07.040-libre.pdf20160306-22960-1eui2x6?1457298651=\u0026response-content-disposition=attachment%3B+filename%3DRetinopathy_in_stunted_black_tiger_shrim.pdf\u0026Expires=1732802456\u0026Signature=PL-BajW-RadVJ9fQus80y1E6J542Uerx9ctLaLv19PyOIGe8LpvgFK84aYAA4GKdXBLtav-RdE9LgY5aEXWOq-2Wz6c2atX9FSmP3XkWhl-ockWqHnNwZMtj7qDmNLI-9SG9j9s5NCauPzIToEavUzBiY5u49cEUpIObHIEtMt74t67gMMn2OBzyUU1yG4x1ItOvI8xfXV3P0OzvtLQ9lE7sngifPN2OSeRsxJRRVzQGjfKcvpeZ-MAlvX6hCMi7NUQbh4-AKgxL5KSy3XK2kVTqaU-FeYKttyRqiO-Fz6yz7C513hTV3xZWT44FW9sKlP2Prvno3sBtoinZODHirA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":7049,"name":"Crustacea","url":"https://www.academia.edu/Documents/in/Crustacea"},{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy"},{"id":23848,"name":"Aquaculture","url":"https://www.academia.edu/Documents/in/Aquaculture"},{"id":82145,"name":"Virus","url":"https://www.academia.edu/Documents/in/Virus"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction"},{"id":127957,"name":"Penaeus Monodon","url":"https://www.academia.edu/Documents/in/Penaeus_Monodon"},{"id":170652,"name":"Fisheries Sciences","url":"https://www.academia.edu/Documents/in/Fisheries_Sciences"},{"id":409570,"name":"Onion","url":"https://www.academia.edu/Documents/in/Onion"},{"id":973494,"name":"Reverse Transcriptase","url":"https://www.academia.edu/Documents/in/Reverse_Transcriptase"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15068660"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15068660/Serotonin_stimulates_ovarian_maturation_and_spawning_in_the_black_tiger_shrimp_Penaeus_monodon"><img alt="Research paper thumbnail of Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon" class="work-thumbnail" src="https://attachments.academia-assets.com/43605089/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15068660/Serotonin_stimulates_ovarian_maturation_and_spawning_in_the_black_tiger_shrimp_Penaeus_monodon">Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KanokpanWongprasert">Kanokpan Wongprasert</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://mahidol.academia.edu/SomlukAsuvapongpatana">Somluk Asuvapongpatana</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/BoonsirmWithyachumnarnkul">Boonsirm Withyachumnarnkul</a></span></div><div class="wp-workCard_item"><span>Aquaculture</span><span>, 2006</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="be54b20d7da50141471a3f75b3beb0b5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":43605089,"asset_id":15068660,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/43605089/download_file?st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&st=MTczMjc5ODg1Niw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15068660"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15068660"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15068660; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "be54b20d7da50141471a3f75b3beb0b5" } } $('.js-work-strip[data-work-id=15068660]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15068660,"title":"Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon","translated_title":"","metadata":{"grobid_abstract":"Serotonin (5-hydroxytryptamine, 5HT) has been reported to induce ovarian maturation and spawning in the crayfish Procambarus clarkii and white Pacific shrimp Litopenaeus vannamei. The aim of this study was to explore the role of exogenous 5HT on the reproductive performance of the black tiger shrimp Penaeus monodon. 5HT solution was injected into domesticated P. monodon broodstock at 50 μg/g body weight and ovarian maturation and spawning were recorded. The presence of 5HT in the ovary and oviduct of P. monodon was also studied by immunohistochemistry and its levels in the ovary by enzyme link immunoabsorbance assay (ELISA). The 5HT-injected P. monodon developed ovarian maturation and spawning rate at the level comparable to that of unilateral eyestalk-ablated shrimp. Hatching rate and the amount of nauplii produced per spawner were also significantly higher in the 5HT-injected shrimp, compared to the eyestalk-ablated shrimp. 5HT-positive reactions were found in the follicular cells of pre-vitellogenic oocytes, in the cytoplasm of early vitellogenic oocytes and on the cell membrane and cytoplasm of late vitellogenic oocytes. 5HT in the ovary was present at 3.53 ± 0.26 ng/mg protein level in previtellogenic stage and increased to 17.03 ± 0.57 ng/mg protein level in the mature stage of the ovary. The results suggest a significant role of 5HT, possibly directly on the ovary and oviduct, on the reproductive function of female P. monodon.","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Aquaculture","grobid_abstract_attachment_id":43605089},"translated_abstract":null,"internal_url":"https://www.academia.edu/15068660/Serotonin_stimulates_ovarian_maturation_and_spawning_in_the_black_tiger_shrimp_Penaeus_monodon","translated_internal_url":"","created_at":"2015-08-21T00:50:32.327-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34098982,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":4880151,"work_id":15068660,"tagging_user_id":34098982,"tagged_user_id":34271684,"co_author_invite_id":1108792,"email":"k***n@mahidol.ac.th","display_order":0,"name":"Kanokpan Wongprasert","title":"Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus 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