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GENEWIZ from Azenta | Oligos Retained for Sanger Sequencing
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style='color:#585F69;font-size:14px'>Exome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services' style='color:#585F69;font-size:14px'>Amplicon Sequencing Services</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a 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bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a 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Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' 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We synthesize, dilute, organize, and store your primers for up to a year for use with future Sanger sequencing orders. This service is designed for the bench scientist with simplicity and convenience in mind.</p> <p> </p> <a rel="noopener noreferrer" href="https://clims4.genewiz.com/OlgOrder?lobId=4&serviceId=410" target="_blank"><button>Place an Order</button></a> <a href="https://www.genewiz.com/en/Public/Resources/FAQs/FAQs-Oligo"><button>Oligo FAQ</button></a> </div> <div class="oligo-content-box" id="oligo-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02l4rSy0" target="_blank"><img alt="" src="/-/media/10013-D_Customer-Referral_WebBanRight1.ashx?la=en&hash=2F3AE4702AA3E83AD20D27ED70E249A396EBDECA" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>How are oligos used in Sanger sequencing?</h2> <p>Oligos are used in Sanger sequencing as primers to initiate DNA synthesis and as terminal nucleotides to terminate further DNA synthesis.</p> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="container"> <div class="headline-center"> <h2>Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Save time on ordering and aliquoting primers </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Standard, desalted oligos up to 60 nt at 25 or 50 nmol scale </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Includes storage at Azenta for up to one year </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Expedited turnaround available for Boston-area researchers </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <p style="text-align: center;"> </p> <style> .table-bordered tbody tr td { vertical-align: middle; } </style> <p> </p> <div class="container margin-top-50 margin-bottom-50" style="text-align: center;"> <h2>Turnaround Time</h2> <div class="table-responsive"> <table class="table table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> </tr> </tbody> </table> <table class="table table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> </tr> </tbody> </table> <table class="table table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td> <h3 class="color-light">Order Placed</h3> </td> <td colspan="2"> <h3 class="color-light" style="text-align: center;">US (Non-Boston Area)</h3> </td> </tr> <tr> <td>Before 3pm ET</td> <td colspan="2">Ready for sequencing the next business day*</td> </tr> <tr class="panel-heading"> <td> <h3 class="color-light">Order Placed</h3> </td> <td colspan="2"> <h3 class="color-light">Greater Boston Area</h3> </td> </tr> <tr> <td> Before Noon</td> <td colspan="2">Ready same-day for overnight sequencing*</td> </tr> <tr> <td>Noon - 8pm</td> <td colspan="2">Ready for sequencing the next business day*</td> </tr> </tbody> </table> </div> <div class="table-responsive" style="text-align: left;"> <p style="margin-top: 0pt; margin-bottom: 0pt; margin-left: 0in; text-align: left;"><span style="color: black;">*Larger orders or sequencing oligos longer than 30 bases may require additional turnaround time. 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