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Aynı yıl, New York Üniversitesi’nde Mikrobiyoloji-İmmunobiyoloji alanlarında Doktora çalışması yapmak üzere tam burslu olarak hak kazandı. Özellikle insan bağışıklık sistemi hücreleri ve vücudumuzda bulunan bakteri türleri arasındaki ilişkiler üzerinde Doktora tez çalışmasını Eylül 2017’de tamamlayarak Ph.D. (Doktora) ünvanını aldı. 2015 Ocak ayında The Jackson Laboratory-Genomic Medicine Enstitüsünde Pre-Doctoral Associate ünvanıyla araştırmalarına devam etti. Ve insan bağışıklık sistemi hücrelerinde CRISPR genom modifikasyonu tekniklerini geliştirme projesinde görev aldı. Bu çalışmalardan yola çıkarak Ekim 2017’den Ekim 2020'ye kadar kanser türlerine karşı genetiği değiştirilmiş CAR-T hücre terapileri ve genetik hastalıklara (örneğin, Duchenne Musküler Distrofi) karşı genetik tedaviler üretmek üzere Acıbadem LabCell Hücre Laboratuvar’ında AR-GE Birim Sorumlusu olarak çalışmalarına devam etmiştir.Sentetik biyoloji, genetik mühendisliği ve moleküler biyoloji alanında lisans ve doktora dönemlerini kapsayan geniş bir bilgi ve tecrübeye sahiptir. Hâlihazırda, uluslararası nadir hastalıklar genetik tedavi yarışması (radichal.com) ve DNA tabanlı kriptoloji, DNA barkod, sağlık hizmetleri ve blockchain teknolojisi kullanan platformlar geliştiren hiDNA girişiminin (hi-dna.com) kurucu direktörüdür. <br /><br />Hâlihazırda, dijital verilerin DNA dizileri içerisinde depolanabileceği ve blockchain teknolojisi ile birlikte şifrelenebileceği platformlar çalışmak üzere HiDNA girişimi Sanofi PharmUp-Workinton programının finalinde Jüri Özel Ödülünü kazanmıştır.<br /><br />--------------------------------------------------------------------------------<br /><br />Cihan Tastan got his B.Sc. degree in METU (Middle East Technical University, Turkey) at Molecular Biology and Genetics Department in 2011. In the same year, he started his Ph.D. studies at Microbiology Department in Medical School of New York University. He accomplished his Ph.D. thesis at 2017, which is about understanding the relationship between the human immune system and microbiota in which trillions of bacteria naturally reside in the human body.<br /><br />In addition to his thesis, he studied CRISPR gene editing technologies in human immune cell types at The Jackson Laboratory-Genomic Medicine Institute between 2015 and 2017. He with the Dr. Unutmaz's laboratory group has developed an efficient system to genetically manipulate human immune T cells using CRISPR gene editing toolbox for interrogation of the immune system and for demonstrating potential therapeutic advances that can be utilized against cancer and autoimmune deficiencies. His papers about CRISPR editing in human immune T cell studies have been published at the Journal of Immunology and Nature Mucosal Immunology.<br />Since October of 2017, he has been Research and Development Supervisor of Acibadem Labcell Laboratory, Istanbul. He is studying cell and gene therapies on several cancer types and Duchene Muscular Dystrophy. <br />----------------------------------------------------------------------------------------<br /><span class="u-fw700">Supervisors: </span>Derya Unutmaz and Ercument Ovali<br /><b>Address: </b>New York, New York, United States<br /><div class="js-profile-less-about u-linkUnstyled u-tcGrayDarker u-textDecorationUnderline u-displayNone">less</div></div></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span><a class="ri-more-link js-profile-ri-list-card" data-click-track="profile-user-info-primary-research-interest" data-has-card-for-ri-list="20976874">View All (12)</a></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="20976874" href="https://www.academia.edu/Documents/in/Molecular_Biology"><div id="js-react-on-rails-context" style="display:none" 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backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Scientific Papers" id="Scientific Papers"><h3 class="profile--tab_heading_container">Scientific Papers by Cihan Tastan</h3></div><div class="js-work-strip profile--work_container" data-work-id="50712322"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/50712322/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice"><img alt="Research paper thumbnail of Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice" class="work-thumbnail" src="https://attachments.academia-assets.com/68589782/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/50712322/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice">Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice</a></div><div class="wp-workCard_item"><span>Scientific Reports</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development fo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization for prolonged periods. In this study, a gamma-irradiated inactivated virus vaccine does not require an extra purification process, unlike the chemically inactivated vaccines. Hence, the novelty of our vaccine candidate (OZG-38.61.3) is that it is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. Efficiency and safety dose (either 10 13 or 10 14 viral RNA copy per dose) of OZG-38.61.3 was initially determined in BALB/c mice. This was followed by testing the immunogenicity and protective efficacy of the vaccine. Human ACE2-encoding transgenic mice were immunized and then infected with the SARS-CoV-2 virus for the challenge test. This study shows that vaccinated mice have lowered SARS-CoV-2 viral RNA copy numbers both in oropharyngeal specimens and in the histological analysis of the lung tissues along with humoral and cellular immune responses, including the neutralizing antibodies similar to those shown in BALB/c mice without substantial toxicity. Subsequently, plans are being</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c4dd6a30cdb04ebb0971fe7825484c44" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":68589782,"asset_id":50712322,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/68589782/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50712322"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50712322"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50712322; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50712322]").text(description); $(".js-view-count[data-work-id=50712322]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50712322; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='50712322']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 50712322, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c4dd6a30cdb04ebb0971fe7825484c44" } } $('.js-work-strip[data-work-id=50712322]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":50712322,"title":"Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice","translated_title":"","metadata":{"doi":"10.1038/s41598-021-95086-4","abstract":"The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. 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href="https://www.academia.edu/49069265/The_Overall_Consequence_of_Antiviral_Drugs_Given_to_Pregnant_Women_with_COVID_19">The Overall Consequence of Antiviral Drugs Given to Pregnant Women with COVID-19</a></div><div class="wp-workCard_item"><span>Journal of Bacteriology & Parasitology</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">During pregnancy, the anatomical structure of the respiratory system changes, and the virus trans...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">During pregnancy, the anatomical structure of the respiratory system changes, and the virus transmitted by droplets and aerosols are more easily inhaled and difficult to remove by pregnant women. Women are generally more susceptible to various pregnancy-related complications and respiratory pathogens, increasing the risk of developing adverse pregnancy and neonatal outcomes. Anecdotal evidence suggests that pregnant women do not appear to differ from the general population in terms of disease transmission, and to date, there is no evidence of vertical transmission from mother to fetus. However, in another study, it is known that members of the coronavirus family are responsible for serious complications such as miscarriage, fetal growth restriction, and congenital anomalies during pregnancy. To date, only a few studies have reported relatively higher rates of adverse birth outcomes in women affected by SARS-CoV-2 infection in late pregnancy. This literature review presents the use of drugs used for the treatment of COVID-19 disease in pregnancy and pregnancy outcomes. It is also aimed to examine the effective control and management of SARS CoV-2 infection in the pregnancy, delivery, and postpartum period in line with the existing literature and guide health personals. Large-scale epidemiological studies are needed to evaluate the course of the infection during pregnancy and the effects of the drugs used on pregnancy and fetus.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a4c88f1d31633988bf58c6466520dab4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":67460627,"asset_id":49069265,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/67460627/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="49069265"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="49069265"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 49069265; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=49069265]").text(description); $(".js-view-count[data-work-id=49069265]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 49069265; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='49069265']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 49069265, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a4c88f1d31633988bf58c6466520dab4" } } $('.js-work-strip[data-work-id=49069265]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":49069265,"title":"The Overall Consequence of Antiviral Drugs Given to Pregnant Women with COVID-19","translated_title":"","metadata":{"abstract":"During pregnancy, the anatomical structure of the respiratory system changes, and the virus transmitted by droplets and aerosols are more easily inhaled and difficult to remove by pregnant women. Women are generally more susceptible to various pregnancy-related complications and respiratory pathogens, increasing the risk of developing adverse pregnancy and neonatal outcomes. Anecdotal evidence suggests that pregnant women do not appear to differ from the general population in terms of disease transmission, and to date, there is no evidence of vertical transmission from mother to fetus. However, in another study, it is known that members of the coronavirus family are responsible for serious complications such as miscarriage, fetal growth restriction, and congenital anomalies during pregnancy. To date, only a few studies have reported relatively higher rates of adverse birth outcomes in women affected by SARS-CoV-2 infection in late pregnancy. This literature review presents the use of drugs used for the treatment of COVID-19 disease in pregnancy and pregnancy outcomes. It is also aimed to examine the effective control and management of SARS CoV-2 infection in the pregnancy, delivery, and postpartum period in line with the existing literature and guide health personals. Large-scale epidemiological studies are needed to evaluate the course of the infection during pregnancy and the effects of the drugs used on pregnancy and fetus.","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"Journal of Bacteriology \u0026 Parasitology"},"translated_abstract":"During pregnancy, the anatomical structure of the respiratory system changes, and the virus transmitted by droplets and aerosols are more easily inhaled and difficult to remove by pregnant women. Women are generally more susceptible to various pregnancy-related complications and respiratory pathogens, increasing the risk of developing adverse pregnancy and neonatal outcomes. Anecdotal evidence suggests that pregnant women do not appear to differ from the general population in terms of disease transmission, and to date, there is no evidence of vertical transmission from mother to fetus. However, in another study, it is known that members of the coronavirus family are responsible for serious complications such as miscarriage, fetal growth restriction, and congenital anomalies during pregnancy. To date, only a few studies have reported relatively higher rates of adverse birth outcomes in women affected by SARS-CoV-2 infection in late pregnancy. This literature review presents the use of drugs used for the treatment of COVID-19 disease in pregnancy and pregnancy outcomes. It is also aimed to examine the effective control and management of SARS CoV-2 infection in the pregnancy, delivery, and postpartum period in line with the existing literature and guide health personals. Large-scale epidemiological studies are needed to evaluate the course of the infection during pregnancy and the effects of the drugs used on pregnancy and fetus.","internal_url":"https://www.academia.edu/49069265/The_Overall_Consequence_of_Antiviral_Drugs_Given_to_Pregnant_Women_with_COVID_19","translated_internal_url":"","created_at":"2021-05-29T15:46:51.619-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[],"downloadable_attachments":[{"id":67460627,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/67460627/thumbnails/1.jpg","file_name":"the_overall_consequence_of_antiviral_drugs_given_to_pregnant_women_with_covid19.pdf","download_url":"https://www.academia.edu/attachments/67460627/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_Overall_Consequence_of_Antiviral_Dru.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/67460627/the_overall_consequence_of_antiviral_drugs_given_to_pregnant_women_with_covid19-libre.pdf?1622331097=\u0026response-content-disposition=attachment%3B+filename%3DThe_Overall_Consequence_of_Antiviral_Dru.pdf\u0026Expires=1732704099\u0026Signature=aFmNyOWYsUcYk8bGz4wdX697WR2PzBEwscLwj98w-HsJzJawrLLCLloCuz9NWhkjWat4gWZSYuNUho0NuWnbSaQCelrwfsbsZE3hgKbEbx0oJkCVTMvtw2wPwf7HhNe9UWarHojja94WfD6SW33s8bnSdk1xAq8wctMzIDIrzdoswyXG1mQRo6PeDUW6gnoNGhbvrfC-6NnUetzAoBpF1lapAfA5Z7S8hcJFg~CWAGk28LuXvUPxd0vve98jAAPlWu-NudwM8vXfhBKZ9lVBE1iaKIyGDPeycnOfbPo9x9aKypZdMqaYhqaQzekDViH3udHK54Qf6zsEnyQFSLRm1w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_Overall_Consequence_of_Antiviral_Drugs_Given_to_Pregnant_Women_with_COVID_19","translated_slug":"","page_count":4,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"TlJTWnN0Mi9EWXZ3UzMvdnRLVHlzYVlUYUtGcFVuS0hGMFlWaXZJTncvZFZOcXBlSEtoV1ZUNitCNEh0ekFMei0tckVNM0d4VUswVFEwWDRxZUpWRGxFdz09--328dec41b8851a77e6c8e534a0806994d5ade7c0"},"attachments":[{"id":67460627,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/67460627/thumbnails/1.jpg","file_name":"the_overall_consequence_of_antiviral_drugs_given_to_pregnant_women_with_covid19.pdf","download_url":"https://www.academia.edu/attachments/67460627/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_Overall_Consequence_of_Antiviral_Dru.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/67460627/the_overall_consequence_of_antiviral_drugs_given_to_pregnant_women_with_covid19-libre.pdf?1622331097=\u0026response-content-disposition=attachment%3B+filename%3DThe_Overall_Consequence_of_Antiviral_Dru.pdf\u0026Expires=1732704099\u0026Signature=aFmNyOWYsUcYk8bGz4wdX697WR2PzBEwscLwj98w-HsJzJawrLLCLloCuz9NWhkjWat4gWZSYuNUho0NuWnbSaQCelrwfsbsZE3hgKbEbx0oJkCVTMvtw2wPwf7HhNe9UWarHojja94WfD6SW33s8bnSdk1xAq8wctMzIDIrzdoswyXG1mQRo6PeDUW6gnoNGhbvrfC-6NnUetzAoBpF1lapAfA5Z7S8hcJFg~CWAGk28LuXvUPxd0vve98jAAPlWu-NudwM8vXfhBKZ9lVBE1iaKIyGDPeycnOfbPo9x9aKypZdMqaYhqaQzekDViH3udHK54Qf6zsEnyQFSLRm1w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="45488617"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/45488617/Preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates"><img alt="Research paper thumbnail of Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates" class="work-thumbnail" src="https://attachments.academia-assets.com/65985763/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/45488617/Preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates">Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates</a></div><div class="wp-workCard_item"><span>Scientific Reports</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no v...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, SARS-CoV-2 vaccines have become crucial for reducing morbidity and mortality. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. The candidate vaccines in this study were OZG-3861 version 1 (V1), an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1), a GM-CSF adjuvant added vaccine. The candidate vaccines were applied intradermally to BALB/c mice to assess toxicity and immunogenicity. Preliminary results in vaccinated mice are reported in this study. Especially, the vaccine models containing GM-CSF caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature, when considered in terms of T and B cell responses. Another important finding was that the presence of adjuvant was more important in T cell in comparison with B cell response. Vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study shows that the vaccines are effective and leads us to start the challenge test to investigate the gamma-irradiated inactivated vaccine candidates for infective SARS-CoV-2 virus in humanized ACE2 + mice.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8b669e506ed29860174b2156e08ffda1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":65985763,"asset_id":45488617,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/65985763/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="45488617"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="45488617"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 45488617; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=45488617]").text(description); $(".js-view-count[data-work-id=45488617]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 45488617; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='45488617']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 45488617, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8b669e506ed29860174b2156e08ffda1" } } $('.js-work-strip[data-work-id=45488617]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":45488617,"title":"Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates","translated_title":"","metadata":{"doi":"10.1038/s41598-021-83930-6","abstract":"COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. 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Vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study shows that the vaccines are effective and leads us to start the challenge test to investigate the gamma-irradiated inactivated vaccine candidates for infective SARS-CoV-2 virus in humanized ACE2 + mice.","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"Scientific Reports"},"translated_abstract":"COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, SARS-CoV-2 vaccines have become crucial for reducing morbidity and mortality. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. The candidate vaccines in this study were OZG-3861 version 1 (V1), an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1), a GM-CSF adjuvant added vaccine. The candidate vaccines were applied intradermally to BALB/c mice to assess toxicity and immunogenicity. Preliminary results in vaccinated mice are reported in this study. Especially, the vaccine models containing GM-CSF caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature, when considered in terms of T and B cell responses. Another important finding was that the presence of adjuvant was more important in T cell in comparison with B cell response. Vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study shows that the vaccines are effective and leads us to start the challenge test to investigate the gamma-irradiated inactivated vaccine candidates for infective SARS-CoV-2 virus in humanized ACE2 + mice.","internal_url":"https://www.academia.edu/45488617/Preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates","translated_internal_url":"","created_at":"2021-03-12T06:40:56.237-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[{"id":36339834,"work_id":45488617,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":7160198,"email":"c***n@acibademlabcell.com.tr","display_order":1,"name":"Cihan Tastan","title":"Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine 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wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/44803738/CRISPR_Of_Things_Applications_and_Challenges_of_the_Most_Popular_Gene_Editing_Tool_in_the_Fields_of_Health_Agriculture_and_Environment">CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://akdeniz.academia.edu/KenanTurgut">Kenan Turgut</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://atauni.academia.edu/KamilHAL%C4%B0LO%C4%9ELU">Kamil HALİLOĞLU</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://adnanmenderes.academia.edu/KemalBenlioglu">Kemal Benlioglu</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://comu.academia.edu/KemalMelihTASKIN">Kemal Melih TASKIN</a></span></div><div class="wp-workCard_item"><span>International Journal of Innovative Approaches in Agricultural Research</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Almost all cells of any living organism contain DNA, a hereditary molecule that passes from gener...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Almost all cells of any living organism contain DNA, a hereditary molecule that passes from generation to generation during reproduction. The term "genome" generally refers to the total DNA sequences in an organism. The genome consists of DNA sequences called "gene", which plays a role in the basic biological processes involved in many phenotypic and genotypic characteristics, such as performing cellular functions, controlling numbers and species, regulating energy production, metabolism, and combating diseases. Gene editing is the process of pre-designing and modifying a particular DNA sequence in a targeted gene. The most widely used technique is CRISPR-Cas technology. For this purpose, the DNA helix is cut at a certain point, to form a double-strand break (DSB), and naturally existing cellular repair mechanisms repair the DSB. Modes of the repair mechanisms may affect the gene function. When DSB is formed, gene editing techniques can be applied to remove, insert, or replace a newly modified sequence using a synthetic donor template DNA. In developed and developing countries, CRISPR-Cas studies in addition to research and development studies are rapidly increasing. In addition to increasing population, changing weather conditions, declining farmland, increasing biotic and abiotic stresses are other important barriers to agricultural production, food, and feed supply. In this report, CRISPR-Cas applications are introduced in detail from the studies that carried out gene modifications in the fields of health, animals, plants, microorganisms, and food supply. Besides, these technologies and applications have been examined in terms of world biosafety legislation and the scientific risk assessment of the products developed using the CRISPR-Cas technique.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="463a049aa56c9be6b61c75819c680172" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":65301681,"asset_id":44803738,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/65301681/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44803738"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44803738"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44803738; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44803738]").text(description); $(".js-view-count[data-work-id=44803738]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44803738; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44803738']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44803738, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "463a049aa56c9be6b61c75819c680172" } } $('.js-work-strip[data-work-id=44803738]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44803738,"title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment","translated_title":"","metadata":{"doi":"10.29329/ijiasr.2020.312.6","abstract":"Almost all cells of any living organism contain DNA, a hereditary molecule that passes from generation to generation during reproduction. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="44701276"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/44701276/Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics"><img alt="Research paper thumbnail of Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics" class="work-thumbnail" src="https://attachments.academia-assets.com/65178708/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/44701276/Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics">Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/berberburak">burak berber</a></span></div><div class="wp-workCard_item"><span>Gene Therapy</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease cause...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV-2) (Prec Clin Med 2020;3:9-13, Lancet 2020;395:497-506, N. Engl J Med 2020a;382:1199-207, Nature 2020;579:270-3). SARS-CoV-2 surveillance is essential to controlling widespread transmission. However, there are several challenges associated with the diagnostic of the COVID-19 during the current outbreak (Liu and Li (2019), Nature 2020;579:265-9, N. Engl J Med 2020;382:727-33). Firstly, the high number of cases overwhelms diagnostic test capacity and proposes the need for a rapid solution for sample processing (Science 2018;360:444-8). Secondly, SARS-CoV-2 is closely related to other important coronavirus species and subspecies, so detection assays can give false-positive results if they are not efficiently specific to SARS-CoV-2. Thirdly, patients with suspected SARS-CoV-2 infection sometimes have a different respiratory viral infection or co-infections with SARS-CoV-2 and other respiratory viruses (MedRxiv 2020a;1-18). Confirmation of the COVID-19 is performed mainly by virus isolation followed by RT-PCR and sequencing (N. Engl J Med 2020;382:727-33, MedRxiv 2020a, Turkish J Biol 2020;44:192-202). The emergence and outbreak of the novel coronavirus highlighted the urgent need for new therapeutic technologies that are fast, precise, stable, easy to manufacture, and target-specific for surveillance and treatment. Molecular biology tools that include gene-editing approaches such as CRISPR-Cas12/13-based SHERLOCK, DETECTR, CARVER and PAC-MAN, antisense oligonucleotides, antisense peptide nucleic acids, ribozymes, aptamers, and RNAi silencing approaches produced with cutting-edge scientific advances compared to conventional diagnostic or treatment methods could be vital in COVID-19 and other future outbreaks. Thus, in this review, we will discuss potent the molecular biology approaches that can revolutionize diagnostic of viral infections and therapies to fight COVID-19 in a highly specific, stable, and efficient way. Highlights • SARS-CoV-2 is an RNA virus that could be targeted and diagnosed with novel CRISPR approaches. • ASO therapy targeting transcript encoding a viral protein or genomic RNA itself could be developed as a response to the SARS-CoV-2 pandemic.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c22858b6a747dd1fe395733e8dd6e21c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":65178708,"asset_id":44701276,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/65178708/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44701276"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44701276"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44701276; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44701276]").text(description); $(".js-view-count[data-work-id=44701276]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44701276; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44701276']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44701276, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c22858b6a747dd1fe395733e8dd6e21c" } } $('.js-work-strip[data-work-id=44701276]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44701276,"title":"Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics","translated_title":"","metadata":{"doi":"10.1038/s41434-020-00209-7","abstract":"The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV-2) (Prec Clin Med 2020;3:9-13, Lancet 2020;395:497-506, N. Engl J Med 2020a;382:1199-207, Nature 2020;579:270-3). SARS-CoV-2 surveillance is essential to controlling widespread transmission. However, there are several challenges associated with the diagnostic of the COVID-19 during the current outbreak (Liu and Li (2019), Nature 2020;579:265-9, N. Engl J Med 2020;382:727-33). Firstly, the high number of cases overwhelms diagnostic test capacity and proposes the need for a rapid solution for sample processing (Science 2018;360:444-8). Secondly, SARS-CoV-2 is closely related to other important coronavirus species and subspecies, so detection assays can give false-positive results if they are not efficiently specific to SARS-CoV-2. Thirdly, patients with suspected SARS-CoV-2 infection sometimes have a different respiratory viral infection or co-infections with SARS-CoV-2 and other respiratory viruses (MedRxiv 2020a;1-18). Confirmation of the COVID-19 is performed mainly by virus isolation followed by RT-PCR and sequencing (N. Engl J Med 2020;382:727-33, MedRxiv 2020a, Turkish J Biol 2020;44:192-202). The emergence and outbreak of the novel coronavirus highlighted the urgent need for new therapeutic technologies that are fast, precise, stable, easy to manufacture, and target-specific for surveillance and treatment. Molecular biology tools that include gene-editing approaches such as CRISPR-Cas12/13-based SHERLOCK, DETECTR, CARVER and PAC-MAN, antisense oligonucleotides, antisense peptide nucleic acids, ribozymes, aptamers, and RNAi silencing approaches produced with cutting-edge scientific advances compared to conventional diagnostic or treatment methods could be vital in COVID-19 and other future outbreaks. Thus, in this review, we will discuss potent the molecular biology approaches that can revolutionize diagnostic of viral infections and therapies to fight COVID-19 in a highly specific, stable, and efficient way. Highlights • SARS-CoV-2 is an RNA virus that could be targeted and diagnosed with novel CRISPR approaches. • ASO therapy targeting transcript encoding a viral protein or genomic RNA itself could be developed as a response to the SARS-CoV-2 pandemic.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Gene Therapy"},"translated_abstract":"The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV-2) (Prec Clin Med 2020;3:9-13, Lancet 2020;395:497-506, N. Engl J Med 2020a;382:1199-207, Nature 2020;579:270-3). SARS-CoV-2 surveillance is essential to controlling widespread transmission. However, there are several challenges associated with the diagnostic of the COVID-19 during the current outbreak (Liu and Li (2019), Nature 2020;579:265-9, N. Engl J Med 2020;382:727-33). Firstly, the high number of cases overwhelms diagnostic test capacity and proposes the need for a rapid solution for sample processing (Science 2018;360:444-8). Secondly, SARS-CoV-2 is closely related to other important coronavirus species and subspecies, so detection assays can give false-positive results if they are not efficiently specific to SARS-CoV-2. Thirdly, patients with suspected SARS-CoV-2 infection sometimes have a different respiratory viral infection or co-infections with SARS-CoV-2 and other respiratory viruses (MedRxiv 2020a;1-18). Confirmation of the COVID-19 is performed mainly by virus isolation followed by RT-PCR and sequencing (N. Engl J Med 2020;382:727-33, MedRxiv 2020a, Turkish J Biol 2020;44:192-202). The emergence and outbreak of the novel coronavirus highlighted the urgent need for new therapeutic technologies that are fast, precise, stable, easy to manufacture, and target-specific for surveillance and treatment. 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Highlights • SARS-CoV-2 is an RNA virus that could be targeted and diagnosed with novel CRISPR approaches. • ASO therapy targeting transcript encoding a viral protein or genomic RNA itself could be developed as a response to the SARS-CoV-2 pandemic.","internal_url":"https://www.academia.edu/44701276/Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics","translated_internal_url":"","created_at":"2020-12-14T05:12:42.070-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[{"id":36055193,"work_id":44701276,"tagging_user_id":20976874,"tagged_user_id":16435221,"co_author_invite_id":null,"email":"b***0@gmail.com","display_order":1,"name":"burak berber","title":"Gene editing and RNAi approaches for COVID-19 diagnostics and 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href="https://www.academia.edu/44393166/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice"><img alt="Research paper thumbnail of Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice" class="work-thumbnail" src="https://attachments.academia-assets.com/64799180/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/44393166/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice">Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/DeryaKan%C3%A7a%C4%9Fi">Derya Kançaği</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/GamzeTumentemur">Gamze Tumentemur</a></span></div><div class="wp-workCard_item"><span>BioRxiv </span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The SARS-CoV-2 virus caused one of the severest pandemic around the world. The vaccine developmen...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The SARS-CoV-2 virus caused one of the severest pandemic around the world. The vaccine development for urgent use became more of an issue during the pandemic. An inactivated virus formulated vaccines such as Hepatitis A, inactivated polio, and influenza has been proven to be a reliable approach for immunization for long years. In this pandemic, we produced an inactivated SARS-CoV-2 vaccine candidate by modification of the oldest but the most experienced method that can be produced quickly and tested easily rather than the recombinant vaccines. Here, we optimized an inactivated virus vaccine which includes the gamma irradiation process for the inactivation as an alternative to classical chemical inactivation methods so that there is no extra purification required. Also, we applied the vaccine candidate (OZG-38.61.3) using the intradermal route in mice which decreased the requirement of a higher concentration of inactivated virus for proper immunization unlike most of the classical inactivated vaccine treatments. Thus, the novelty of our vaccine candidate (OZG-38.61.3) is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. We first determined the efficiency and safety dose (either 1013 or 1014 viral copy per dose) of the OZG-38.61.3 in Balb/c mice. Next, to test the immunogenicity and protective efficacy of the OZG-38.61.3, we immunized human ACE2-encoding transgenic mice and infected them with a dose of infective SARS-CoV-2 virus for the challenge test. We showed that the vaccinated mice showed lowered SARS-CoV-2 viral copy number in oropharyngeal specimens along with humoral and cellular immune responses against the SARS-CoV-2, including the neutralizing antibodies similar to those shown in Balb/c mice without substantial toxicity. This study encouraged us towards a new promising strategy for inactivated vaccine development (OZG-38.61.3) and the Phase 1 clinical trial for the COVID-19 pandemic.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d4a40ec4c69425148f4e1407fb7125ad" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64799180,"asset_id":44393166,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64799180/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44393166"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44393166"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44393166; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44393166]").text(description); $(".js-view-count[data-work-id=44393166]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44393166; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44393166']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44393166, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d4a40ec4c69425148f4e1407fb7125ad" } } $('.js-work-strip[data-work-id=44393166]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44393166,"title":"Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice","translated_title":"","metadata":{"doi":"10.1101/2020.10.28.356667","abstract":"The SARS-CoV-2 virus caused one of the severest pandemic around the world. The vaccine development for urgent use became more of an issue during the pandemic. An inactivated virus formulated vaccines such as Hepatitis A, inactivated polio, and influenza has been proven to be a reliable approach for immunization for long years. In this pandemic, we produced an inactivated SARS-CoV-2 vaccine candidate by modification of the oldest but the most experienced method that can be produced quickly and tested easily rather than the recombinant vaccines. Here, we optimized an inactivated virus vaccine which includes the gamma irradiation process for the inactivation as an alternative to classical chemical inactivation methods so that there is no extra purification required. Also, we applied the vaccine candidate (OZG-38.61.3) using the intradermal route in mice which decreased the requirement of a higher concentration of inactivated virus for proper immunization unlike most of the classical inactivated vaccine treatments. Thus, the novelty of our vaccine candidate (OZG-38.61.3) is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. We first determined the efficiency and safety dose (either 1013 or 1014 viral copy per dose) of the OZG-38.61.3 in Balb/c mice. Next, to test the immunogenicity and protective efficacy of the OZG-38.61.3, we immunized human ACE2-encoding transgenic mice and infected them with a dose of infective SARS-CoV-2 virus for the challenge test. We showed that the vaccinated mice showed lowered SARS-CoV-2 viral copy number in oropharyngeal specimens along with humoral and cellular immune responses against the SARS-CoV-2, including the neutralizing antibodies similar to those shown in Balb/c mice without substantial toxicity. This study encouraged us towards a new promising strategy for inactivated vaccine development (OZG-38.61.3) and the Phase 1 clinical trial for the COVID-19 pandemic.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"BioRxiv "},"translated_abstract":"The SARS-CoV-2 virus caused one of the severest pandemic around the world. The vaccine development for urgent use became more of an issue during the pandemic. An inactivated virus formulated vaccines such as Hepatitis A, inactivated polio, and influenza has been proven to be a reliable approach for immunization for long years. In this pandemic, we produced an inactivated SARS-CoV-2 vaccine candidate by modification of the oldest but the most experienced method that can be produced quickly and tested easily rather than the recombinant vaccines. Here, we optimized an inactivated virus vaccine which includes the gamma irradiation process for the inactivation as an alternative to classical chemical inactivation methods so that there is no extra purification required. Also, we applied the vaccine candidate (OZG-38.61.3) using the intradermal route in mice which decreased the requirement of a higher concentration of inactivated virus for proper immunization unlike most of the classical inactivated vaccine treatments. Thus, the novelty of our vaccine candidate (OZG-38.61.3) is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. We first determined the efficiency and safety dose (either 1013 or 1014 viral copy per dose) of the OZG-38.61.3 in Balb/c mice. Next, to test the immunogenicity and protective efficacy of the OZG-38.61.3, we immunized human ACE2-encoding transgenic mice and infected them with a dose of infective SARS-CoV-2 virus for the challenge test. We showed that the vaccinated mice showed lowered SARS-CoV-2 viral copy number in oropharyngeal specimens along with humoral and cellular immune responses against the SARS-CoV-2, including the neutralizing antibodies similar to those shown in Balb/c mice without substantial toxicity. This study encouraged us towards a new promising strategy for inactivated vaccine development (OZG-38.61.3) and the Phase 1 clinical trial for the COVID-19 pandemic.","internal_url":"https://www.academia.edu/44393166/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice","translated_internal_url":"","created_at":"2020-10-29T04:33:18.738-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[{"id":35886899,"work_id":44393166,"tagging_user_id":20976874,"tagged_user_id":147482034,"co_author_invite_id":null,"email":"p***3@hotmail.com","display_order":1,"name":"Derya Kançaği","title":"Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic 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data-click-track="profile-work-strip-title" href="https://www.academia.edu/44037453/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection">Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection</a></div><div class="wp-workCard_item"><span>New Microbes and New Infections </span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). It is well known that novel Coronavirus disease 2019 (COVID-19) pneumonia progresses to ARDS and even multiple organ failure. High blood neutrophil levels are an early indicator of COVID-19 and predict severe respiratory diseases. Also it is reported that mucus structure of COVID-19 is very similar to cystic fibrosis due to the accumulation of excessive NET in the lungs. In this study, we showed the recovery of three COVID-19 patients after including Dornase alfa in their treatment. We followed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea, coughing and a decrease in NET formation and SARS-CoV-2 viral load after the treatment. Also here, we share our preliminary results suggesting that Dornase alfa has an anti-viral effect against SARS-CoV-2 infection in a green monkey kidney cell line, Vero, and a bovine kidney cell line, MDBK without determined cytotoxicity on healthy peripheral blood mononuclear cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c4f9f71592db803d5d74dcfe119443d0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64528192,"asset_id":44037453,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64528192/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44037453"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44037453"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44037453; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44037453]").text(description); $(".js-view-count[data-work-id=44037453]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44037453; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44037453']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44037453, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c4f9f71592db803d5d74dcfe119443d0" } } $('.js-work-strip[data-work-id=44037453]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44037453,"title":"Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection","translated_title":"","metadata":{"doi":"10.1016/j.nmni.2020.100756","abstract":"Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). It is well known that novel Coronavirus disease 2019 (COVID-19) pneumonia progresses to ARDS and even multiple organ failure. High blood neutrophil levels are an early indicator of COVID-19 and predict severe respiratory diseases. Also it is reported that mucus structure of COVID-19 is very similar to cystic fibrosis due to the accumulation of excessive NET in the lungs. In this study, we showed the recovery of three COVID-19 patients after including Dornase alfa in their treatment. We followed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea, coughing and a decrease in NET formation and SARS-CoV-2 viral load after the treatment. Also here, we share our preliminary results suggesting that Dornase alfa has an anti-viral effect against SARS-CoV-2 infection in a green monkey kidney cell line, Vero, and a bovine kidney cell line, MDBK without determined cytotoxicity on healthy peripheral blood mononuclear cells.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"New Microbes and New Infections "},"translated_abstract":"Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). It is well known that novel Coronavirus disease 2019 (COVID-19) pneumonia progresses to ARDS and even multiple organ failure. High blood neutrophil levels are an early indicator of COVID-19 and predict severe respiratory diseases. Also it is reported that mucus structure of COVID-19 is very similar to cystic fibrosis due to the accumulation of excessive NET in the lungs. In this study, we showed the recovery of three COVID-19 patients after including Dornase alfa in their treatment. We followed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea, coughing and a decrease in NET formation and SARS-CoV-2 viral load after the treatment. Also here, we share our preliminary results suggesting that Dornase alfa has an anti-viral effect against SARS-CoV-2 infection in a green monkey kidney cell line, Vero, and a bovine kidney cell line, MDBK without determined cytotoxicity on healthy peripheral blood mononuclear cells.","internal_url":"https://www.academia.edu/44037453/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection","translated_internal_url":"","created_at":"2020-09-07T14:09:17.754-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[],"downloadable_attachments":[{"id":64528192,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/64528192/thumbnails/1.jpg","file_name":"1-s2.0-S2052297520301086-main (2).pdf","download_url":"https://www.academia.edu/attachments/64528192/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Preliminary_Report_of_In_vitro_and_In_vi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/64528192/1-s2.0-S2052297520301086-main_%282%29-libre.pdf?1601122504=\u0026response-content-disposition=attachment%3B+filename%3DPreliminary_Report_of_In_vitro_and_In_vi.pdf\u0026Expires=1732739027\u0026Signature=SBrPRQIfwe0nfqFMKr8ma8I0OIiQyPNVPUFihqAmNSgv0ox-aVutaJEtuZUkHAdhif8obN3IZuIFvTcKTvDjalkmzAZWMjYKhXLOispU82mzV-xQonALBtsa~4Icqk~xo6EnH~0~EvJvs6h94fue~QIr9f-N55hbmvF6wDzLiFqysO6BtJ7wYAnV6F-9otewVhdNGsd9W58bBx4upQds4IPLA~Jq90q8JHKuYU1Z3ewtuc-e8WV166LpMmbjcFM2VfJ9c6kqpECU4IkbatxCeVUmwX~CrxmysI3-wZD~RS4j0tem8QrMVGmj-hBPh7ARqj7D50FyNxPElR~8FHzjpg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection","translated_slug":"","page_count":1,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":64528192,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/64528192/thumbnails/1.jpg","file_name":"1-s2.0-S2052297520301086-main (2).pdf","download_url":"https://www.academia.edu/attachments/64528192/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Preliminary_Report_of_In_vitro_and_In_vi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/64528192/1-s2.0-S2052297520301086-main_%282%29-libre.pdf?1601122504=\u0026response-content-disposition=attachment%3B+filename%3DPreliminary_Report_of_In_vitro_and_In_vi.pdf\u0026Expires=1732739027\u0026Signature=SBrPRQIfwe0nfqFMKr8ma8I0OIiQyPNVPUFihqAmNSgv0ox-aVutaJEtuZUkHAdhif8obN3IZuIFvTcKTvDjalkmzAZWMjYKhXLOispU82mzV-xQonALBtsa~4Icqk~xo6EnH~0~EvJvs6h94fue~QIr9f-N55hbmvF6wDzLiFqysO6BtJ7wYAnV6F-9otewVhdNGsd9W58bBx4upQds4IPLA~Jq90q8JHKuYU1Z3ewtuc-e8WV166LpMmbjcFM2VfJ9c6kqpECU4IkbatxCeVUmwX~CrxmysI3-wZD~RS4j0tem8QrMVGmj-hBPh7ARqj7D50FyNxPElR~8FHzjpg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[{"id":9111290,"url":"https://www.sciencedirect.com/science/article/pii/S2052297520301086"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="44020845"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/44020845/Preliminary_report_of_preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates_SK_01_version_1_and_OZG_3861_version_1"><img alt="Research paper thumbnail of Preliminary report of preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates, SK-01 version 1 and OZG-3861 version 1" class="work-thumbnail" src="https://attachments.academia-assets.com/64356882/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/44020845/Preliminary_report_of_preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates_SK_01_version_1_and_OZG_3861_version_1">Preliminary report of preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates, SK-01 version 1 and OZG-3861 version 1</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://istanbul.academia.edu/UgurOzbek">Ugur Ozbek</a></span></div><div class="wp-workCard_item"><span>BioRxiv </span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The COVID-19 outbreak caused by SARS-CoV-2 has created an unprecedented health crisis since there...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The COVID-19 outbreak caused by SARS-CoV-2 has created an unprecedented health crisis since there is no coronavirus vaccine in the market due to the novelty of this virus. Therefore, SARS-CoV-2 vaccines have become very important to reduce morbidity and mortality. At this point, inactivated vaccines are important because the straightforward process of existing infrastructure used for several licensed human vaccines can be used for SARS-CoV-2. Inactive vaccines provide an antigenic presentation similar to that when they encounter invasive virus particles of the immune system. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. Our candidate OZG-3861 version 1 (V1) is an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1) is the GM-CSF adjuvant added vaccine candidate. We applied the candidates intradermal to BALB/c mice to assess the toxicity and immunogenicity of the OZG-3861 V1 and SK-01 V1. Here, we report our preliminary results in vaccinated mice. When considered in terms of T and B cell responses, it was observed that especially the vaccine models containing GM-CSF as an adjuvant caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature. Another finding showed that the presence of adjuvant is more important in T cell response rather than B cell. The vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study encouraged us to start the challenge test using infective SARS-CoV-2 viruses and our second version of gamma-irradiated inactivated vaccine candidates in humanized ACE2+ mice.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f798e80c458a6360fd5c053083342415" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64356882,"asset_id":44020845,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64356882/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44020845"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44020845"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44020845; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44020845]").text(description); $(".js-view-count[data-work-id=44020845]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44020845; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44020845']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44020845, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f798e80c458a6360fd5c053083342415" } } $('.js-work-strip[data-work-id=44020845]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44020845,"title":"Preliminary report of preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates, SK-01 version 1 and OZG-3861 version 1","translated_title":"","metadata":{"doi":"10.1101/2020.09.04.277426","abstract":"The COVID-19 outbreak caused by SARS-CoV-2 has created an unprecedented health crisis since there is no coronavirus vaccine in the market due to the novelty of this virus. Therefore, SARS-CoV-2 vaccines have become very important to reduce morbidity and mortality. At this point, inactivated vaccines are important because the straightforward process of existing infrastructure used for several licensed human vaccines can be used for SARS-CoV-2. Inactive vaccines provide an antigenic presentation similar to that when they encounter invasive virus particles of the immune system. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. Our candidate OZG-3861 version 1 (V1) is an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1) is the GM-CSF adjuvant added vaccine candidate. We applied the candidates intradermal to BALB/c mice to assess the toxicity and immunogenicity of the OZG-3861 V1 and SK-01 V1. Here, we report our preliminary results in vaccinated mice. 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Therefore, SARS-CoV-2 vaccines have become very important to reduce morbidity and mortality. At this point, inactivated vaccines are important because the straightforward process of existing infrastructure used for several licensed human vaccines can be used for SARS-CoV-2. Inactive vaccines provide an antigenic presentation similar to that when they encounter invasive virus particles of the immune system. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. Our candidate OZG-3861 version 1 (V1) is an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1) is the GM-CSF adjuvant added vaccine candidate. We applied the candidates intradermal to BALB/c mice to assess the toxicity and immunogenicity of the OZG-3861 V1 and SK-01 V1. Here, we report our preliminary results in vaccinated mice. When considered in terms of T and B cell responses, it was observed that especially the vaccine models containing GM-CSF as an adjuvant caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature. Another finding showed that the presence of adjuvant is more important in T cell response rather than B cell. The vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. 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Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients" class="work-thumbnail" src="https://attachments.academia-assets.com/64983046/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/43822999/Preclinical_Assessment_of_Efficacy_and_Safety_Analysis_of_CAR_T_Cells_ISIKOK_19_targeting_CD19_expressing_B_Cells_for_the_First_Turkish_Academic_Clinical_Trial_with_Relapsed_refractory_ALL_and_NHL_Patients">Preclinical Assessment of Efficacy and Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Didem%C3%87ak%C4%B1rsoy">Didem Çakırsoy</a></span></div><div class="wp-workCard_item"><span>Turkish Journal of Hematology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Relapsed and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin L...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Relapsed and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin Lymphoma (NHL) are the focus of studies in hematological cancers. Treatment of these malignancies has undergone recent transformation with the development of new gene therapy and molecular biology techniques, which are safer and well-tolerated therapeutic approaches. The CD19 antigen is the most studied therapeutic target in these hematological cancers. For this purpose, we used a lentiviral vector encoding the CD19 antigen-specific antibody head (FMC63) conjugated with CD8-CD28-CD3ζ sequence as a chimeric antigen receptor (CAR) along with a truncated form of EGFR (EGFRt) on human T lymphocytes (CAR-T). In this study, we pre-clinically assessed efficacy and safety of the manufactured CAR-T cells both from healthy donor and ALL/NHL patient’ peripheral blood mononuclear cells, namely ISIKOK-19. Furthermore, we showed significant enhancement of CAR lentivirus transduction efficacy in T cells using BX-795, an inhibitor of the signaling molecule TBK1/IKKƐ in order to cut the cost of CAR-T cell production. In addition, ISIKOK-19 cells demonstrated a significant high level of cytotoxicity specifically against a CD19+ B-lymphocyte cancer model, RAJI cells, in NOD/SCID mice. This is the first report of preclinical assessment of efficacy and safety analysis of CAR-T cells (ISIKOK-19) targeting CD19-expressing B cells in relapsed/refractory ALL and NHL patients in Turkey.  Keywords: Chimeric Antigen Receptor, CD19, CAR-T, Immunotherapy Preclinical Assessment of Efficacy and Safety Analysis of CAR-T cells (ISIKOK-19) targeting CD19-expressing B cells for the first Turkish academic clinical trial with relapsed/refractory ALL and NHL patients</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a631f9a5ae85041038bb5c6d9c88898b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64983046,"asset_id":43822999,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64983046/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="43822999"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="43822999"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 43822999; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=43822999]").text(description); $(".js-view-count[data-work-id=43822999]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 43822999; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='43822999']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 43822999, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a631f9a5ae85041038bb5c6d9c88898b" } } $('.js-work-strip[data-work-id=43822999]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":43822999,"title":"Preclinical Assessment of Efficacy and Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients","translated_title":"","metadata":{"doi":"10.4274/tjh.galenos.2020.2020.0070","abstract":"Relapsed and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin Lymphoma (NHL) are the focus of studies in hematological cancers. 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class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/43399947/SARS_CoV_2_isolation_and_propagation_from_Turkish_COVID_19_patients">SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SelenAbanuz">Selen Abanuz</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KorayYal%C3%A7%C4%B1n">Koray Yalçın</a></span></div><div class="wp-workCard_item"><span>Turkish Journal of Biology </span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6749ad56d35302e4e11058893da5e774" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":63697379,"asset_id":43399947,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/63697379/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="43399947"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="43399947"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 43399947; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=43399947]").text(description); $(".js-view-count[data-work-id=43399947]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 43399947; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='43399947']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 43399947, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6749ad56d35302e4e11058893da5e774" } } $('.js-work-strip[data-work-id=43399947]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":43399947,"title":"SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients","translated_title":"","metadata":{"doi":"10.3906/biy-2004-113","abstract":"The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Turkish Journal of Biology "},"translated_abstract":"The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.","internal_url":"https://www.academia.edu/43399947/SARS_CoV_2_isolation_and_propagation_from_Turkish_COVID_19_patients","translated_internal_url":"","created_at":"2020-06-21T11:24:19.080-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[{"id":35061730,"work_id":43399947,"tagging_user_id":20976874,"tagged_user_id":33780531,"co_author_invite_id":null,"email":"b***g@gmail.com","affiliation":"Ataturk University","display_order":1,"name":"bulut yurtsever","title":"SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients"},{"id":35061731,"work_id":43399947,"tagging_user_id":20976874,"tagged_user_id":162521372,"co_author_invite_id":7054046,"email":"s***z@gmail.com","display_order":2,"name":"Selen Abanuz","title":"SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients"},{"id":35061732,"work_id":43399947,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":7054047,"email":"u***s@acibademlabcell.com.tr","display_order":3,"name":"Utku Seyis","title":"SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients"},{"id":35061733,"work_id":43399947,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":7054048,"email":"m***m@acibademlabcell.com.tr","display_order":4,"name":"Mülazim Yildirim","title":"SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients"},{"id":35061734,"work_id":43399947,"tagging_user_id":20976874,"tagged_user_id":37580818,"co_author_invite_id":null,"email":"k***c@gmail.com","display_order":5,"name":"Koray Yalçın","title":"SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients"},{"id":35061735,"work_id":43399947,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":7054049,"email":"e***i@acibadem.com","display_order":6,"name":"Ercüment Ovali","title":"SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients"}],"downloadable_attachments":[{"id":63697379,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/63697379/thumbnails/1.jpg","file_name":"biy-44-si-1-8-2004-113_620200621-105134-1fh7vzk.pdf","download_url":"https://www.academia.edu/attachments/63697379/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"SARS_CoV_2_isolation_and_propagation_fro.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/63697379/biy-44-si-1-8-2004-113_620200621-105134-1fh7vzk-libre.pdf?1592764808=\u0026response-content-disposition=attachment%3B+filename%3DSARS_CoV_2_isolation_and_propagation_fro.pdf\u0026Expires=1732739027\u0026Signature=hF5MKihVfGyeCD3zpOktvb2~EOQBu~V89LUVeNgTM-BuzTIS9MgM8wAMmqJ6UxNgeKxZ3bR5oiAsitObQebHFbZeLt9vUBSndRMdeGMbAm0Pje8ZtPISOpgV0hgPB720P0eypcg0gDkieQyrBPvFVz1d1mgP~EPhbEqw68wuway9BOVvn7Q1WgabGH5SCldwhUXJkSfOYPExHOYullOsZpEZQ4fi-P2oGETrKV0v8zTg1~VfXwrbF8Yrny~z45yW4Zxt8HgkxI3226~UjnXrabgP2TzPgmOXIqZvHSkos8j0P0Xc5EoKGrwVNgHDgD1fAXsPO2Li4psIn7xD0Ka4TQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"SARS_CoV_2_isolation_and_propagation_from_Turkish_COVID_19_patients","translated_slug":"","page_count":11,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":63697379,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/63697379/thumbnails/1.jpg","file_name":"biy-44-si-1-8-2004-113_620200621-105134-1fh7vzk.pdf","download_url":"https://www.academia.edu/attachments/63697379/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"SARS_CoV_2_isolation_and_propagation_fro.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/63697379/biy-44-si-1-8-2004-113_620200621-105134-1fh7vzk-libre.pdf?1592764808=\u0026response-content-disposition=attachment%3B+filename%3DSARS_CoV_2_isolation_and_propagation_fro.pdf\u0026Expires=1732739027\u0026Signature=hF5MKihVfGyeCD3zpOktvb2~EOQBu~V89LUVeNgTM-BuzTIS9MgM8wAMmqJ6UxNgeKxZ3bR5oiAsitObQebHFbZeLt9vUBSndRMdeGMbAm0Pje8ZtPISOpgV0hgPB720P0eypcg0gDkieQyrBPvFVz1d1mgP~EPhbEqw68wuway9BOVvn7Q1WgabGH5SCldwhUXJkSfOYPExHOYullOsZpEZQ4fi-P2oGETrKV0v8zTg1~VfXwrbF8Yrny~z45yW4Zxt8HgkxI3226~UjnXrabgP2TzPgmOXIqZvHSkos8j0P0Xc5EoKGrwVNgHDgD1fAXsPO2Li4psIn7xD0Ka4TQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="43041910"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/43041910/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection"><img alt="Research paper thumbnail of Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection" class="work-thumbnail" src="https://attachments.academia-assets.com/63302019/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/43041910/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection">Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection</a></div><div class="wp-workCard_item"><span>JMIR Preprints</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). Coronavirus disease 2019(COVID-19) pandemic affected more than two million people over the world, resulting in unprecedented health, social and economic crises. The COVID-19, viral pneumonia that progresses to ARDS and even multiple organ failure, is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High blood neutrophil levels are an early indicator of SARS-CoV-2infection and predict severe respiratory diseases. A similar mucus structure is detected in COVID-19patients due to the accumulation of excessive NET in the lungs. Here, we show our preliminary results with dornase alfa that may have an in-vitro anti-viral effect against SARS-CoV-2 infection ina bovine kidney cell line, MDBK without drug toxicity on healthy adult peripheral blood mononuclear cells. In this preliminary study, we also showed that dornase alfa can promote clearance of NET formation in both an in-vitro and three COVID-19 cases who showed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea and coughing.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="172e5e8f5bbac46c06b73a7d42f36ba2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":63302019,"asset_id":43041910,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/63302019/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="43041910"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="43041910"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 43041910; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=43041910]").text(description); $(".js-view-count[data-work-id=43041910]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 43041910; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='43041910']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 43041910, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "172e5e8f5bbac46c06b73a7d42f36ba2" } } $('.js-work-strip[data-work-id=43041910]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":43041910,"title":"Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection","translated_title":"","metadata":{"doi":"10.2196/preprints.20200","abstract":"Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). Coronavirus disease 2019(COVID-19) pandemic affected more than two million people over the world, resulting in unprecedented health, social and economic crises. The COVID-19, viral pneumonia that progresses to ARDS and even multiple organ failure, is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High blood neutrophil levels are an early indicator of SARS-CoV-2infection and predict severe respiratory diseases. A similar mucus structure is detected in COVID-19patients due to the accumulation of excessive NET in the lungs. Here, we show our preliminary results with dornase alfa that may have an in-vitro anti-viral effect against SARS-CoV-2 infection ina bovine kidney cell line, MDBK without drug toxicity on healthy adult peripheral blood mononuclear cells. In this preliminary study, we also showed that dornase alfa can promote clearance of NET formation in both an in-vitro and three COVID-19 cases who showed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea and coughing.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"JMIR Preprints"},"translated_abstract":"Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). Coronavirus disease 2019(COVID-19) pandemic affected more than two million people over the world, resulting in unprecedented health, social and economic crises. The COVID-19, viral pneumonia that progresses to ARDS and even multiple organ failure, is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High blood neutrophil levels are an early indicator of SARS-CoV-2infection and predict severe respiratory diseases. A similar mucus structure is detected in COVID-19patients due to the accumulation of excessive NET in the lungs. Here, we show our preliminary results with dornase alfa that may have an in-vitro anti-viral effect against SARS-CoV-2 infection ina bovine kidney cell line, MDBK without drug toxicity on healthy adult peripheral blood mononuclear cells. In this preliminary study, we also showed that dornase alfa can promote clearance of NET formation in both an in-vitro and three COVID-19 cases who showed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea and coughing.","internal_url":"https://www.academia.edu/43041910/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection","translated_internal_url":"","created_at":"2020-05-13T19:09:49.606-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[],"downloadable_attachments":[{"id":63302019,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/63302019/thumbnails/1.jpg","file_name":"preprint-20200-submitted20200513-62035-8879ry.pdf","download_url":"https://www.academia.edu/attachments/63302019/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Preliminary_Report_of_In_vitro_and_In_vi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/63302019/preprint-20200-submitted20200513-62035-8879ry-libre.pdf?1589422926=\u0026response-content-disposition=attachment%3B+filename%3DPreliminary_Report_of_In_vitro_and_In_vi.pdf\u0026Expires=1732704101\u0026Signature=Ur8tDlTNMV2o5luKew2~kIcKrjYLLtVIUuQ0qW1Bf5Po-G55PxlNobgvdlu2Ct7diO~FVc3UyBewXu58~YjYzkbyiLL94VbkLiN-2tV8XsAIageOttFTNKE4gFzzjk1gSHihl3KptghQxVwlzejR6qu0ArheM4lUr-MfSIM3c6aWcY6STwzzp5t9ZYHpmtDAueBLYHx39Vd3XazcHcL5jANIyA3~ow~EAZHqfCO4ob4y0zKQtABFcu3YMiOC8eycjv1aKUN8yKZH7hwqqNmHTHYnjEvixjpwcNP7e1AXZB7K7c9Tb5O6zVUxqVae6p42CoghMcjfJTsjJLKD~RUbfw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection","translated_slug":"","page_count":29,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"Ylhrc2I2SWIyd0E2QTgyU0JsNXNUdUxzTXVFVXVqNXVUL0NzVzQrdFA0ZG1XNFVhV3FIbHRTVVVxb1EweWI5VC0tZm1XZUVYcURYVndJZEpURnk3WEEvZz09--90a359e66e9f7cba8ab57a242052446933950186"},"attachments":[{"id":63302019,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/63302019/thumbnails/1.jpg","file_name":"preprint-20200-submitted20200513-62035-8879ry.pdf","download_url":"https://www.academia.edu/attachments/63302019/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Preliminary_Report_of_In_vitro_and_In_vi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/63302019/preprint-20200-submitted20200513-62035-8879ry-libre.pdf?1589422926=\u0026response-content-disposition=attachment%3B+filename%3DPreliminary_Report_of_In_vitro_and_In_vi.pdf\u0026Expires=1732704101\u0026Signature=Ur8tDlTNMV2o5luKew2~kIcKrjYLLtVIUuQ0qW1Bf5Po-G55PxlNobgvdlu2Ct7diO~FVc3UyBewXu58~YjYzkbyiLL94VbkLiN-2tV8XsAIageOttFTNKE4gFzzjk1gSHihl3KptghQxVwlzejR6qu0ArheM4lUr-MfSIM3c6aWcY6STwzzp5t9ZYHpmtDAueBLYHx39Vd3XazcHcL5jANIyA3~ow~EAZHqfCO4ob4y0zKQtABFcu3YMiOC8eycjv1aKUN8yKZH7hwqqNmHTHYnjEvixjpwcNP7e1AXZB7K7c9Tb5O6zVUxqVae6p42CoghMcjfJTsjJLKD~RUbfw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="42845858"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/42845858/SARS_CoV_2_Isolation_and_Propagation_from_Turkish_COVID_19_patients"><img alt="Research paper thumbnail of SARS-CoV-2 Isolation and Propagation from Turkish COVID-19 patients" class="work-thumbnail" src="https://attachments.academia-assets.com/63081606/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/42845858/SARS_CoV_2_Isolation_and_Propagation_from_Turkish_COVID_19_patients">SARS-CoV-2 Isolation and Propagation from Turkish COVID-19 patients</a></div><div class="wp-workCard_item"><span>bioRxiv</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The novel coronavirus pneumonia, which was named later as Coronavirus Disease 2019 (COVID-19), is...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The novel coronavirus pneumonia, which was named later as Coronavirus Disease 2019 (COVID-19), is caused by the Severe Acute Respiratory Syndrome Coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. Today, over eight thousand cases in Turkey, and two million cases around the world have been declared. Due to the urgent need for vaccine studies and drug discoveries, isolation of the virus is crucial. Here, we report one of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study suggests replication methodology and cell culture tropism of the virus that will be available to the research communities.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="59c157b4b282a345fd0c9464bfa6a733" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":63081606,"asset_id":42845858,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/63081606/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="42845858"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="42845858"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42845858; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42845858]").text(description); $(".js-view-count[data-work-id=42845858]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 42845858; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='42845858']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 42845858, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "59c157b4b282a345fd0c9464bfa6a733" } } $('.js-work-strip[data-work-id=42845858]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":42845858,"title":"SARS-CoV-2 Isolation and Propagation from Turkish COVID-19 patients","translated_title":"","metadata":{"doi":"10.1101/2020.04.23.056309","abstract":"The novel coronavirus pneumonia, which was named later as Coronavirus Disease 2019 (COVID-19), is caused by the Severe Acute Respiratory Syndrome Coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. Today, over eight thousand cases in Turkey, and two million cases around the world have been declared. Due to the urgent need for vaccine studies and drug discoveries, isolation of the virus is crucial. Here, we report one of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study suggests replication methodology and cell culture tropism of the virus that will be available to the research communities.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"bioRxiv"},"translated_abstract":"The novel coronavirus pneumonia, which was named later as Coronavirus Disease 2019 (COVID-19), is caused by the Severe Acute Respiratory Syndrome Coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. Today, over eight thousand cases in Turkey, and two million cases around the world have been declared. Due to the urgent need for vaccine studies and drug discoveries, isolation of the virus is crucial. Here, we report one of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study suggests replication methodology and cell culture tropism of the virus that will be available to the research communities.","internal_url":"https://www.academia.edu/42845858/SARS_CoV_2_Isolation_and_Propagation_from_Turkish_COVID_19_patients","translated_internal_url":"","created_at":"2020-04-24T19:31:57.794-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[],"downloadable_attachments":[{"id":63081606,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/63081606/thumbnails/1.jpg","file_name":"2020.04.23.056309v1.full20200424-22254-1buoul2.pdf","download_url":"https://www.academia.edu/attachments/63081606/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"SARS_CoV_2_Isolation_and_Propagation_fro.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/63081606/2020.04.23.056309v1.full20200424-22254-1buoul2-libre.pdf?1587782609=\u0026response-content-disposition=attachment%3B+filename%3DSARS_CoV_2_Isolation_and_Propagation_fro.pdf\u0026Expires=1732739027\u0026Signature=fAw5d-SGrJfMy~msQmk6hBqwsbHjm8Mq2XaYxwO0eJV8TA1wKVZL5R~ZA4VdzUIR0UbLtlkn8Z~zqRV0rGm~A6dcskcolZoVo7aACR-c6UJknWiZn5aXu8XPvalck1Jzs2XCQQxlMVmgbXBNDyjE8mOskFaUNdBboaHBjslTATNCY~C7qaNLuleJu16OimZbnCzf2i6yr0iBux8TPE-9Xs7rGBiQx2dPojoyZWmvRDngttk4xN7PUe9Mdm5-7cxDE6bL-EVyya1hqFdefMP~DUZRdigmqGilzetdG0G4Az3U2rwc8RBdIdmaqx77yB28yFP~IFh4lBBMySDPpM6uJQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"SARS_CoV_2_Isolation_and_Propagation_from_Turkish_COVID_19_patients","translated_slug":"","page_count":21,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":63081606,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/63081606/thumbnails/1.jpg","file_name":"2020.04.23.056309v1.full20200424-22254-1buoul2.pdf","download_url":"https://www.academia.edu/attachments/63081606/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"SARS_CoV_2_Isolation_and_Propagation_fro.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/63081606/2020.04.23.056309v1.full20200424-22254-1buoul2-libre.pdf?1587782609=\u0026response-content-disposition=attachment%3B+filename%3DSARS_CoV_2_Isolation_and_Propagation_fro.pdf\u0026Expires=1732739027\u0026Signature=fAw5d-SGrJfMy~msQmk6hBqwsbHjm8Mq2XaYxwO0eJV8TA1wKVZL5R~ZA4VdzUIR0UbLtlkn8Z~zqRV0rGm~A6dcskcolZoVo7aACR-c6UJknWiZn5aXu8XPvalck1Jzs2XCQQxlMVmgbXBNDyjE8mOskFaUNdBboaHBjslTATNCY~C7qaNLuleJu16OimZbnCzf2i6yr0iBux8TPE-9Xs7rGBiQx2dPojoyZWmvRDngttk4xN7PUe9Mdm5-7cxDE6bL-EVyya1hqFdefMP~DUZRdigmqGilzetdG0G4Az3U2rwc8RBdIdmaqx77yB28yFP~IFh4lBBMySDPpM6uJQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="42666338"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/42666338/Alternative_Therapies_to_Antibiotics_CRISPR_Cas_antimicrobials"><img alt="Research paper thumbnail of Alternative Therapies to Antibiotics: CRISPR-Cas antimicrobials" class="work-thumbnail" src="https://attachments.academia-assets.com/62874038/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/42666338/Alternative_Therapies_to_Antibiotics_CRISPR_Cas_antimicrobials">Alternative Therapies to Antibiotics: CRISPR-Cas antimicrobials</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ege.academia.edu/ay%C5%9Feg%C3%BClate%C5%9F">Ayşegül Ateş</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SafakErmertcan">Safak Ermertcan</a></span></div><div class="wp-workCard_item"><span>Gene Editing</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antibiotics affect specific mechanisms of bacteria by targeting cellular pathways or f...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antibiotics  affect  specific  mechanisms  of  bacteria  by  targeting  cellular  pathways  or  functions  such  as  function  of  cell membrane, blockage of cell wall synthesis, protein or nucleic acid synthesis. They can’t selectively  kill targeted  pathogens  in the  mixed  microbial  population  with  these  mechanisms.  Antibiotics  cause  dysfunction  not  only  of  the  bacteria  that  cause infection but also of the beneficial microbiota members in the host. Currently, there is no specific antibiotic strategy targeting only virulent or antibiotic-resistant bacteria. Current antibiotic strategies aren’t specific; resistant bacteria allow spread of the resistance  genes  in  the  bacterial  population.  Recently,  new  molecular  techniques  have  been  introduced  to deal  with antimicrobial  resistance.  Researchers  could  knock  out  plasmid-mediated  antibiotic  resistance  genes  in  order  to  prevent  the spread of resistance. This review will discuss antibiotic resistance, CRISPR-Cas9 gene editing mechanism and its applications against bacteria itself, which will be an important method to prevent the clonal spread of resistant strains, providing a unique solution to the global problem.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1f5226fb2b20504eed2bc0021bbb70a0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":62874038,"asset_id":42666338,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/62874038/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="42666338"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="42666338"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42666338; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42666338]").text(description); $(".js-view-count[data-work-id=42666338]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 42666338; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='42666338']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 42666338, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1f5226fb2b20504eed2bc0021bbb70a0" } } $('.js-work-strip[data-work-id=42666338]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":42666338,"title":"Alternative Therapies to Antibiotics: CRISPR-Cas antimicrobials","translated_title":"","metadata":{"doi":"10.29228/genediting.41450","abstract":"Antibiotics affect specific mechanisms of bacteria by targeting cellular pathways or functions such as function of cell membrane, blockage of cell wall synthesis, protein or nucleic acid synthesis. They can’t selectively kill targeted pathogens in the mixed microbial population with these mechanisms. Antibiotics cause dysfunction not only of the bacteria that cause infection but also of the beneficial microbiota members in the host. Currently, there is no specific antibiotic strategy targeting only virulent or antibiotic-resistant bacteria. Current antibiotic strategies aren’t specific; resistant bacteria allow spread of the resistance genes in the bacterial population. Recently, new molecular techniques have been introduced to deal with antimicrobial resistance. Researchers could knock out plasmid-mediated antibiotic resistance genes in order to prevent the spread of resistance. This review will discuss antibiotic resistance, CRISPR-Cas9 gene editing mechanism and its applications against bacteria itself, which will be an important method to prevent the clonal spread of resistant strains, providing a unique solution to the global problem.\n","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Gene Editing"},"translated_abstract":"Antibiotics affect specific mechanisms of bacteria by targeting cellular pathways or functions such as function of cell membrane, blockage of cell wall synthesis, protein or nucleic acid synthesis. They can’t selectively kill targeted pathogens in the mixed microbial population with these mechanisms. Antibiotics cause dysfunction not only of the bacteria that cause infection but also of the beneficial microbiota members in the host. Currently, there is no specific antibiotic strategy targeting only virulent or antibiotic-resistant bacteria. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="37253761"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/37253761/Tuning_of_human_MAIT_cell_activation_by_commensal_bacteria_species_and_MR1_dependent_T_cell_presentation"><img alt="Research paper thumbnail of Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation" class="work-thumbnail" src="https://attachments.academia-assets.com/61328942/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37253761/Tuning_of_human_MAIT_cell_activation_by_commensal_bacteria_species_and_MR1_dependent_T_cell_presentation">Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ElizabethFleming11">Elizabeth Fleming</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AnitaVoigt">Anita Voigt</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JuliaOh5">Julia Oh</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/DeryaUnutmaz">Derya Unutmaz</a></span></div><div class="wp-workCard_item"><span>Mucosal Immunology </span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. However, it is unclear whether MAIT cells discriminate between many species of the human microbiota. To address this, we developed an in vitro functional assay through human T cells engineered for MAIT-TCRs (eMAIT-TCRs) stimulated by MR1-expressing antigen-presenting cells (APCs). We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded the riboflavin pathway were stimulatory for MAIT-TCRs. Most species that were high stimulators belonged to Bacteroidetes and Proteobacteria phyla, whereas low/non-stimulator species were primarily Actinobacteria or Firmicutes. Activation of MAIT cells by high-vs low-stimulating bacteria also correlated with the level of riboflavin they secreted or after bacterial infection of macrophages. Remarkably, we found that human T-cell subsets can also present riboflavin metabolites to MAIT cells in a MR1-restricted fashion. This T–T cell-mediated signaling also induced IFNγ, TNF and granzyme B from MAIT cells, albeit at lower level than professional APC. These findings suggest that MAIT cells can discriminate and categorize complex human microbiota through computation of TCR signals depending on antigen load and presenting cells, and fine-tune their functional responses.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e5201d8b9be6d3c94615f81e9b153ffa" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61328942,"asset_id":37253761,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61328942/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37253761"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37253761"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37253761; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37253761]").text(description); $(".js-view-count[data-work-id=37253761]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37253761; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37253761']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37253761, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e5201d8b9be6d3c94615f81e9b153ffa" } } $('.js-work-strip[data-work-id=37253761]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37253761,"title":"Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation","translated_title":"","metadata":{"doi":"10.1038/s41385-018-0072-x","abstract":"Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. However, it is unclear whether MAIT cells discriminate between many species of the human microbiota. To address this, we developed an in vitro functional assay through human T cells engineered for MAIT-TCRs (eMAIT-TCRs) stimulated by MR1-expressing antigen-presenting cells (APCs). We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded the riboflavin pathway were stimulatory for MAIT-TCRs. Most species that were high stimulators belonged to Bacteroidetes and Proteobacteria phyla, whereas low/non-stimulator species were primarily Actinobacteria or Firmicutes. Activation of MAIT cells by high-vs low-stimulating bacteria also correlated with the level of riboflavin they secreted or after bacterial infection of macrophages. Remarkably, we found that human T-cell subsets can also present riboflavin metabolites to MAIT cells in a MR1-restricted fashion. This T–T cell-mediated signaling also induced IFNγ, TNF and granzyme B from MAIT cells, albeit at lower level than professional APC. These findings suggest that MAIT cells can discriminate and categorize complex human microbiota through computation of TCR signals depending on antigen load and presenting cells, and fine-tune their functional responses.","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"Mucosal Immunology "},"translated_abstract":"Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. However, it is unclear whether MAIT cells discriminate between many species of the human microbiota. To address this, we developed an in vitro functional assay through human T cells engineered for MAIT-TCRs (eMAIT-TCRs) stimulated by MR1-expressing antigen-presenting cells (APCs). We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded the riboflavin pathway were stimulatory for MAIT-TCRs. Most species that were high stimulators belonged to Bacteroidetes and Proteobacteria phyla, whereas low/non-stimulator species were primarily Actinobacteria or Firmicutes. Activation of MAIT cells by high-vs low-stimulating bacteria also correlated with the level of riboflavin they secreted or after bacterial infection of macrophages. Remarkably, we found that human T-cell subsets can also present riboflavin metabolites to MAIT cells in a MR1-restricted fashion. This T–T cell-mediated signaling also induced IFNγ, TNF and granzyme B from MAIT cells, albeit at lower level than professional APC. These findings suggest that MAIT cells can discriminate and categorize complex human microbiota through computation of TCR signals depending on antigen load and presenting cells, and fine-tune their functional responses.","internal_url":"https://www.academia.edu/37253761/Tuning_of_human_MAIT_cell_activation_by_commensal_bacteria_species_and_MR1_dependent_T_cell_presentation","translated_internal_url":"","created_at":"2018-08-17T11:41:51.512-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[{"id":31806760,"work_id":37253761,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":6724591,"email":"e***n@jax.org","display_order":1,"name":"Ece Karhan","title":"Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell 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src="https://attachments.academia-assets.com/61328995/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37094832/Functional_Interrogation_of_Primary_Human_T_Cells_via_CRISPR_Genetic_Editing">Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LindseyPlacek">Lindsey Placek</a></span></div><div class="wp-workCard_item"><span>The Journal of Immunology</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Developing precise and efficient gene editing approaches using CRISPR in primary human T cell sub...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (CD4, CD45, CD95) could be simultaneously and stably deleted in naive, memory, effector, or regulatory T cell (Treg) subsets at very high efficiency. Additionally, nuclease-deficient Cas9, associated with a transcriptional activator or repressor, can downregulate or increase expression of genes in T cells. For example, expression of glycoprotein A repetitions predominant (GARP), a gene that is normally and exclusively expressed on activated Tregs, could be induced on non-Treg effector T cells by nuclease-deficient Cas9 fused to transcriptional activators. Further analysis determined that this approach could be used in mapping promoter sequences involved in gene transcription. Through this CRISPR/Cas9–mediated genetic editing we also demonstrated the feasibility of human T cell functional analysis in several examples: 1) CD95 deletion inhibited T cell apoptosis upon reactivation; 2) deletion of ORAI1, a Ca2+ release–activated channel, abolished Ca2+ influx and cytokine secretion, mimicking natural genetic mutations in immune-deficient patients; and 3) transcriptional activation of CD25 or CD127 expression enhanced cytokine signaling by IL-2 or IL-7, respectively. Taken together, application of the CRISPR toolbox to human T cell subsets has important implications for decoding the mechanisms of their functional outputs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="da1ab496a3de8cd2f2431c42397d6603" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61328995,"asset_id":37094832,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61328995/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37094832"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37094832"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37094832; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37094832]").text(description); $(".js-view-count[data-work-id=37094832]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37094832; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37094832']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37094832, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "da1ab496a3de8cd2f2431c42397d6603" } } $('.js-work-strip[data-work-id=37094832]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37094832,"title":"Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing","translated_title":"","metadata":{"doi":"10.4049/jimmunol.1701616","abstract":"Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (CD4, CD45, CD95) could be simultaneously and stably deleted in naive, memory, effector, or regulatory T cell (Treg) subsets at very high efficiency. Additionally, nuclease-deficient Cas9, associated with a transcriptional activator or repressor, can downregulate or increase expression of genes in T cells. For example, expression of glycoprotein A repetitions predominant (GARP), a gene that is normally and exclusively expressed on activated Tregs, could be induced on non-Treg effector T cells by nuclease-deficient Cas9 fused to transcriptional activators. Further analysis determined that this approach could be used in mapping promoter sequences involved in gene transcription. Through this CRISPR/Cas9–mediated genetic editing we also demonstrated the feasibility of human T cell functional analysis in several examples: 1) CD95 deletion inhibited T cell apoptosis upon reactivation; 2) deletion of ORAI1, a Ca2+ release–activated channel, abolished Ca2+ influx and cytokine secretion, mimicking natural genetic mutations in immune-deficient patients; and 3) transcriptional activation of CD25 or CD127 expression enhanced cytokine signaling by IL-2 or IL-7, respectively. Taken together, application of the CRISPR toolbox to human T cell subsets has important implications for decoding the mechanisms of their functional outputs.","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"The Journal of Immunology"},"translated_abstract":"Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (CD4, CD45, CD95) could be simultaneously and stably deleted in naive, memory, effector, or regulatory T cell (Treg) subsets at very high efficiency. Additionally, nuclease-deficient Cas9, associated with a transcriptional activator or repressor, can downregulate or increase expression of genes in T cells. For example, expression of glycoprotein A repetitions predominant (GARP), a gene that is normally and exclusively expressed on activated Tregs, could be induced on non-Treg effector T cells by nuclease-deficient Cas9 fused to transcriptional activators. Further analysis determined that this approach could be used in mapping promoter sequences involved in gene transcription. Through this CRISPR/Cas9–mediated genetic editing we also demonstrated the feasibility of human T cell functional analysis in several examples: 1) CD95 deletion inhibited T cell apoptosis upon reactivation; 2) deletion of ORAI1, a Ca2+ release–activated channel, abolished Ca2+ influx and cytokine secretion, mimicking natural genetic mutations in immune-deficient patients; and 3) transcriptional activation of CD25 or CD127 expression enhanced cytokine signaling by IL-2 or IL-7, respectively. Taken together, application of the CRISPR toolbox to human T cell subsets has important implications for decoding the mechanisms of their functional outputs.","internal_url":"https://www.academia.edu/37094832/Functional_Interrogation_of_Primary_Human_T_Cells_via_CRISPR_Genetic_Editing","translated_internal_url":"","created_at":"2018-07-22T00:30:31.147-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[{"id":31715593,"work_id":37094832,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":6722456,"email":"l***a@jax.org","display_order":-1,"name":"Lina Kozhaya","title":"Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing"},{"id":31715594,"work_id":37094832,"tagging_user_id":20976874,"tagged_user_id":88041877,"co_author_invite_id":6722457,"email":"l***k@gmail.com","display_order":1,"name":"Lindsey Placek","title":"Functional 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src="https://attachments.academia-assets.com/55277057/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/35416278/PhD_Dissertation_Thesis_Abstract_Tuning_of_Human_Mucosal_Associated_Invariant_T_MAIT_Cell_Function_through_Microbiota_Mediated_T_Cell_Receptor_Signals">PhD Dissertation/Thesis Abstract: Tuning of Human Mucosal Associated Invariant T (MAIT) Cell Function through Microbiota-Mediated T Cell Receptor Signals</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated"> Mucosal-associated invariant T (MAIT) cells are a subset of the T cell population defined by an ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mucosal-associated invariant T (MAIT) cells are a subset of the T cell population defined by an invariant T cell receptor (TCR) that is stimulated by bacterial metabolites. These cells preferentially migrate to mucosal tissues such as gut, liver and skin. MAIT cells are activated by metabolites from the riboflavin (Vitamin B2) biosynthetic pathway that are presented by MHC class 1-related (MR1) molecules expressed on numerous of cell types including antigen presenting cells (APCs) and epithelial cells. The V alpha segment of MAIT TCRs is invariant and composed of Vα7.2, whereas the V beta portion can be variable. MAIT cells are present at very low levels in neonatal blood but significantly increase in adults and show very high variance in their frequency among individuals. While there is evidence that several pathogenic bacteria can activate MAIT cells and that one of their functions is to kill cells with intracellular bacteria, it remains unknown how they discriminate among thousands of commensal bacteria that can also produce Vitamin B2 metabolites. Further, the role of the TCR variable beta region in antigen recognition is still unclear. In this thesis project, we hypothesized that MAIT cells can be stimulated by bacteria that inhabit human mucosal tissues, and that this microbiota-MAIT cell interaction is one of the driving forces in their expansion and variation in the human population. In support of this hypothesis, we observed a reduced proportion of MAIT cells in the blood of perinatally HIV-infected children, which correlated with other microbiota-associated parameters including Th17 cells and inflammatory markers of microbiota alterations. However, it is unclear whether MAIT cells can discriminate bacterial species that reside in the human microbiota, which can produce the riboflavin intermediates. To address this, we developed an in vitro functional assay using human T cells engineered for MAIT-specific TCRs (eMAIT-TCRs) stimulated by MR1-expressing B cell lines presenting the bacterial metabolites. We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded a riboflavin biosynthesis pathway were stimulatory for MAIT-TCRs. Most species that were high-stimulators belonged to Bacteroidetes and Proteobacteria phyla, although with significant variance, whereas low/non-stimulator species were either Actinobacteria or Firmicutes. Furthermore, we determined a wide range of intra-species variation in eMAIT-TCR stimulation capacities of Staphylococcus epidermidis , a skin commensal, which suggests that bacteria can modulate the production capacities of MAIT-TCR stimulatory antigens. Remarkably, we also discovered that human T cells not only express low-levels of MR1 but can also present bacterial metabolites to MAIT cells in an MR1-restricted fashion and trigger TCR signaling to induce cytokine secretion (IFNγ and TNFα), albeit at lower levels. In conclusion, our findings revealed that MAIT cells could discriminate between MR1-restricted, bacteria-derived metabolites through their TCR signaling thresholds. This knowledge paves the way to elucidate the complexity of MAIT cell recognition and its response to the human microbiota, which establishes a framework to explore mechanistic or therapeutic approaches for maintenance of a healthy immunological equilibrium.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="45a5585d8c000033a8d66c1a8d8cfbf1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":55277057,"asset_id":35416278,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/55277057/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="35416278"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="35416278"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 35416278; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=35416278]").text(description); $(".js-view-count[data-work-id=35416278]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 35416278; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='35416278']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 35416278, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "45a5585d8c000033a8d66c1a8d8cfbf1" } } $('.js-work-strip[data-work-id=35416278]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":35416278,"title":"PhD Dissertation/Thesis Abstract: Tuning of Human Mucosal Associated Invariant T (MAIT) Cell Function through Microbiota-Mediated T Cell Receptor Signals","translated_title":"","metadata":{"abstract":" Mucosal-associated invariant T (MAIT) cells are a subset of the T cell population defined by an invariant T cell receptor (TCR) that is stimulated by bacterial metabolites. These cells preferentially migrate to mucosal tissues such as gut, liver and skin. MAIT cells are activated by metabolites from the riboflavin (Vitamin B2) biosynthetic pathway that are presented by MHC class 1-related (MR1) molecules expressed on numerous of cell types including antigen presenting cells (APCs) and epithelial cells. The V alpha segment of MAIT TCRs is invariant and composed of Vα7.2, whereas the V beta portion can be variable. MAIT cells are present at very low levels in neonatal blood but significantly increase in adults and show very high variance in their frequency among individuals. While there is evidence that several pathogenic bacteria can activate MAIT cells and that one of their functions is to kill cells with intracellular bacteria, it remains unknown how they discriminate among thousands of commensal bacteria that can also produce Vitamin B2 metabolites. Further, the role of the TCR variable beta region in antigen recognition is still unclear. In this thesis project, we hypothesized that MAIT cells can be stimulated by bacteria that inhabit human mucosal tissues, and that this microbiota-MAIT cell interaction is one of the driving forces in their expansion and variation in the human population. In support of this hypothesis, we observed a reduced proportion of MAIT cells in the blood of perinatally HIV-infected children, which correlated with other microbiota-associated parameters including Th17 cells and inflammatory markers of microbiota alterations. However, it is unclear whether MAIT cells can discriminate bacterial species that reside in the human microbiota, which can produce the riboflavin intermediates. To address this, we developed an in vitro functional assay using human T cells engineered for MAIT-specific TCRs (eMAIT-TCRs) stimulated by MR1-expressing B cell lines presenting the bacterial metabolites. We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded a riboflavin biosynthesis pathway were stimulatory for MAIT-TCRs. Most species that were high-stimulators belonged to Bacteroidetes and Proteobacteria phyla, although with significant variance, whereas low/non-stimulator species were either Actinobacteria or Firmicutes. Furthermore, we determined a wide range of intra-species variation in eMAIT-TCR stimulation capacities of Staphylococcus epidermidis , a skin commensal, which suggests that bacteria can modulate the production capacities of MAIT-TCR stimulatory antigens. Remarkably, we also discovered that human T cells not only express low-levels of MR1 but can also present bacterial metabolites to MAIT cells in an MR1-restricted fashion and trigger TCR signaling to induce cytokine secretion (IFNγ and TNFα), albeit at lower levels. In conclusion, our findings revealed that MAIT cells could discriminate between MR1-restricted, bacteria-derived metabolites through their TCR signaling thresholds. This knowledge paves the way to elucidate the complexity of MAIT cell recognition and its response to the human microbiota, which establishes a framework to explore mechanistic or therapeutic approaches for maintenance of a healthy immunological equilibrium. "},"translated_abstract":" Mucosal-associated invariant T (MAIT) cells are a subset of the T cell population defined by an invariant T cell receptor (TCR) that is stimulated by bacterial metabolites. These cells preferentially migrate to mucosal tissues such as gut, liver and skin. MAIT cells are activated by metabolites from the riboflavin (Vitamin B2) biosynthetic pathway that are presented by MHC class 1-related (MR1) molecules expressed on numerous of cell types including antigen presenting cells (APCs) and epithelial cells. The V alpha segment of MAIT TCRs is invariant and composed of Vα7.2, whereas the V beta portion can be variable. MAIT cells are present at very low levels in neonatal blood but significantly increase in adults and show very high variance in their frequency among individuals. While there is evidence that several pathogenic bacteria can activate MAIT cells and that one of their functions is to kill cells with intracellular bacteria, it remains unknown how they discriminate among thousands of commensal bacteria that can also produce Vitamin B2 metabolites. Further, the role of the TCR variable beta region in antigen recognition is still unclear. In this thesis project, we hypothesized that MAIT cells can be stimulated by bacteria that inhabit human mucosal tissues, and that this microbiota-MAIT cell interaction is one of the driving forces in their expansion and variation in the human population. In support of this hypothesis, we observed a reduced proportion of MAIT cells in the blood of perinatally HIV-infected children, which correlated with other microbiota-associated parameters including Th17 cells and inflammatory markers of microbiota alterations. However, it is unclear whether MAIT cells can discriminate bacterial species that reside in the human microbiota, which can produce the riboflavin intermediates. To address this, we developed an in vitro functional assay using human T cells engineered for MAIT-specific TCRs (eMAIT-TCRs) stimulated by MR1-expressing B cell lines presenting the bacterial metabolites. We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded a riboflavin biosynthesis pathway were stimulatory for MAIT-TCRs. Most species that were high-stimulators belonged to Bacteroidetes and Proteobacteria phyla, although with significant variance, whereas low/non-stimulator species were either Actinobacteria or Firmicutes. Furthermore, we determined a wide range of intra-species variation in eMAIT-TCR stimulation capacities of Staphylococcus epidermidis , a skin commensal, which suggests that bacteria can modulate the production capacities of MAIT-TCR stimulatory antigens. Remarkably, we also discovered that human T cells not only express low-levels of MR1 but can also present bacterial metabolites to MAIT cells in an MR1-restricted fashion and trigger TCR signaling to induce cytokine secretion (IFNγ and TNFα), albeit at lower levels. In conclusion, our findings revealed that MAIT cells could discriminate between MR1-restricted, bacteria-derived metabolites through their TCR signaling thresholds. This knowledge paves the way to elucidate the complexity of MAIT cell recognition and its response to the human microbiota, which establishes a framework to explore mechanistic or therapeutic approaches for maintenance of a healthy immunological equilibrium. ","internal_url":"https://www.academia.edu/35416278/PhD_Dissertation_Thesis_Abstract_Tuning_of_Human_Mucosal_Associated_Invariant_T_MAIT_Cell_Function_through_Microbiota_Mediated_T_Cell_Receptor_Signals","translated_internal_url":"","created_at":"2017-12-12T23:40:45.957-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":55277057,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/55277057/thumbnails/1.jpg","file_name":"PQDT_Open.pdf","download_url":"https://www.academia.edu/attachments/55277057/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"PhD_Dissertation_Thesis_Abstract_Tuning.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/55277057/PQDT_Open-libre.pdf?1513151175=\u0026response-content-disposition=attachment%3B+filename%3DPhD_Dissertation_Thesis_Abstract_Tuning.pdf\u0026Expires=1732557283\u0026Signature=dhUJcX7N~Bzak7QDpSWW6B~pokG9CRnKHG-WmCSzEEVaOpKtagvfOSGBNjm85mN6ffGNDMDEe2rf2xyNloWE1umP5rMtkryNG4iqt~xSPVLirIJqaxBtCEI5c0Zo87JTHvx5N6vrgqHtxnXY37J~Uq4EFQ~twvd8C9lqQ8Tj~UVfDuBmLR-f-XL3KjZbHn29Z6CGcr~dVUaQvhd6P0MMwRoMXkDxySAXS9HOKdt7J0sRYvd9GDVzR0atjF9nTPtvi4nv8VrOiTZnZ1ia2nI~kuc9q8cmVdzmFkNezV2jIybnNrIih6OcQ~Nbe5R2jxiQ0ogTfSzxyQTDjivIrpoCWw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"PhD_Dissertation_Thesis_Abstract_Tuning_of_Human_Mucosal_Associated_Invariant_T_MAIT_Cell_Function_through_Microbiota_Mediated_T_Cell_Receptor_Signals","translated_slug":"","page_count":2,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":55277057,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/55277057/thumbnails/1.jpg","file_name":"PQDT_Open.pdf","download_url":"https://www.academia.edu/attachments/55277057/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"PhD_Dissertation_Thesis_Abstract_Tuning.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/55277057/PQDT_Open-libre.pdf?1513151175=\u0026response-content-disposition=attachment%3B+filename%3DPhD_Dissertation_Thesis_Abstract_Tuning.pdf\u0026Expires=1732557283\u0026Signature=dhUJcX7N~Bzak7QDpSWW6B~pokG9CRnKHG-WmCSzEEVaOpKtagvfOSGBNjm85mN6ffGNDMDEe2rf2xyNloWE1umP5rMtkryNG4iqt~xSPVLirIJqaxBtCEI5c0Zo87JTHvx5N6vrgqHtxnXY37J~Uq4EFQ~twvd8C9lqQ8Tj~UVfDuBmLR-f-XL3KjZbHn29Z6CGcr~dVUaQvhd6P0MMwRoMXkDxySAXS9HOKdt7J0sRYvd9GDVzR0atjF9nTPtvi4nv8VrOiTZnZ1ia2nI~kuc9q8cmVdzmFkNezV2jIybnNrIih6OcQ~Nbe5R2jxiQ0ogTfSzxyQTDjivIrpoCWw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":4814,"name":"HIV/AIDS","url":"https://www.academia.edu/Documents/in/HIV_AIDS"},{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging"},{"id":53948,"name":"Human Microbiome","url":"https://www.academia.edu/Documents/in/Human_Microbiome"},{"id":166587,"name":"Mucosal Immunology","url":"https://www.academia.edu/Documents/in/Mucosal_Immunology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="28098699"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/28098699/HIV_Infected_Children_Have_Lower_Frequencies_of_CD8_Mucosal_Associated_Invariant_T_MAIT_Cells_that_Correlate_with_Innate_Th17_and_Th22_Cell_Subsets"><img alt="Research paper thumbnail of HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets" class="work-thumbnail" src="https://attachments.academia-assets.com/48413308/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/28098699/HIV_Infected_Children_Have_Lower_Frequencies_of_CD8_Mucosal_Associated_Invariant_T_MAIT_Cells_that_Correlate_with_Innate_Th17_and_Th22_Cell_Subsets">HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets</a></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocom-patib...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocom-patibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and-positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity , MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. In this study we evaluated the frequency of MAIT cells in perinatally HIV-infected children, their response to antiretrovi-ral treatment and their associations with HIV clinical status and related innate and adaptive immune cell subsets with potent antibacterial effector functions. We found HIV+ children between ages 3 to 18 years have significantly decreased CD8+ MAIT cell frequencies compared to uninfected healthy children. Remarkably, CD8 MAIT levels gradually increased with antiretroviral therapy, with greater recovery when treatment is initiated at a young age. Moreover, diminished CD8+ MAIT cell frequencies are associated with low CD4:CD8 ratios and elevated sCD14, suggesting a link with HIV disease progression. Last, CD8+ MAIT cell levels tightly correlate with other antibacterial and mucosa-protective immune subsets, namely, neutrophils, innate-like T cells, and Th17 and Th22 cells. Together these findings suggest that low frequencies of MAIT cells in HIV positive children are part of a concerted disruption to the innate and adaptive immune compartments specialized in sensing and responding to pathogenic or commensal bacteria.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3a8772228cc228525a6688d34d4a3b26" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":48413308,"asset_id":28098699,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/48413308/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="28098699"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="28098699"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 28098699; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=28098699]").text(description); $(".js-view-count[data-work-id=28098699]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 28098699; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='28098699']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 28098699, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3a8772228cc228525a6688d34d4a3b26" } } $('.js-work-strip[data-work-id=28098699]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":28098699,"title":"HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets","translated_title":"","metadata":{"doi":"10.1371/journal.pone.0161786","issue":"8","volume":"11","abstract":"Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocom-patibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and-positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity , MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. In this study we evaluated the frequency of MAIT cells in perinatally HIV-infected children, their response to antiretrovi-ral treatment and their associations with HIV clinical status and related innate and adaptive immune cell subsets with potent antibacterial effector functions. We found HIV+ children between ages 3 to 18 years have significantly decreased CD8+ MAIT cell frequencies compared to uninfected healthy children. Remarkably, CD8 MAIT levels gradually increased with antiretroviral therapy, with greater recovery when treatment is initiated at a young age. Moreover, diminished CD8+ MAIT cell frequencies are associated with low CD4:CD8 ratios and elevated sCD14, suggesting a link with HIV disease progression. Last, CD8+ MAIT cell levels tightly correlate with other antibacterial and mucosa-protective immune subsets, namely, neutrophils, innate-like T cells, and Th17 and Th22 cells. Together these findings suggest that low frequencies of MAIT cells in HIV positive children are part of a concerted disruption to the innate and adaptive immune compartments specialized in sensing and responding to pathogenic or commensal bacteria.","page_numbers":"e0161786","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"PLoS ONE"},"translated_abstract":"Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocom-patibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and-positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity , MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. In this study we evaluated the frequency of MAIT cells in perinatally HIV-infected children, their response to antiretrovi-ral treatment and their associations with HIV clinical status and related innate and adaptive immune cell subsets with potent antibacterial effector functions. We found HIV+ children between ages 3 to 18 years have significantly decreased CD8+ MAIT cell frequencies compared to uninfected healthy children. Remarkably, CD8 MAIT levels gradually increased with antiretroviral therapy, with greater recovery when treatment is initiated at a young age. Moreover, diminished CD8+ MAIT cell frequencies are associated with low CD4:CD8 ratios and elevated sCD14, suggesting a link with HIV disease progression. Last, CD8+ MAIT cell levels tightly correlate with other antibacterial and mucosa-protective immune subsets, namely, neutrophils, innate-like T cells, and Th17 and Th22 cells. Together these findings suggest that low frequencies of MAIT cells in HIV positive children are part of a concerted disruption to the innate and adaptive immune compartments specialized in sensing and responding to pathogenic or commensal bacteria.","internal_url":"https://www.academia.edu/28098699/HIV_Infected_Children_Have_Lower_Frequencies_of_CD8_Mucosal_Associated_Invariant_T_MAIT_Cells_that_Correlate_with_Innate_Th17_and_Th22_Cell_Subsets","translated_internal_url":"","created_at":"2016-08-29T09:43:16.889-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[{"id":23838202,"work_id":28098699,"tagging_user_id":20976874,"tagged_user_id":33263908,"co_author_invite_id":null,"email":"a***n@nyumc.org","display_order":1,"name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="37169570"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/37169570/Modifying_astronauts_microbiome_may_enhance_prevention_from_commensal_bacteria_caused_infections_at_microgravity_condition"><img alt="Research paper thumbnail of Modifying astronauts' microbiome may enhance prevention from commensal bacteria caused infections at microgravity condition" class="work-thumbnail" src="https://attachments.academia-assets.com/57120723/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37169570/Modifying_astronauts_microbiome_may_enhance_prevention_from_commensal_bacteria_caused_infections_at_microgravity_condition">Modifying astronauts' microbiome may enhance prevention from commensal bacteria caused infections at microgravity condition</a></div><div class="wp-workCard_item"><span>The Journal of Brief Ideas</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Since in the 2020s, astronauts will be expected to build a colony at Mars without return. And in ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Since in the 2020s, astronauts will be expected to build a colony at Mars without return. And in a report named 'Microbial responses to<br />microgravity and other low-shear environments' by Nickerson et al. at 2004, it is stated that Salmonella typhimurium, known for causing<br />food-borne illness, can change its genome to become more virulent after just a few days in space. That's why modification of astronauts'<br />microbiome gets importance so that infectious diseases might be prevented and antibiotic tolerance might be reduced.<br />On the other hand, a recent report, named 'Salmonella typhimurium intercepts Escherichia coli signaling to enhance antibiotic tolerance',<br />showed that the intestinal pathogen Salmonella typhimurium increases its antibiotic tolerance in response to the bacterial signaling molecule<br />indole, even though Salmonella typhimurium does not natively produce indole. So that this intestinal pathogen can benefit from indole<br />signaling produced by Escherichia coli and/or other intestinal commensal bacteria.<br /><br />To conclude, modifying the indole pathway of Escherichia coli and/or other commensal bacteria of astronauts with the help of synthetic biology techniques may prevent intestinal bacteria caused infections caused by microgravity.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7cfeec238ea3bb7cc084fc42fed9af40" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":57120723,"asset_id":37169570,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/57120723/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37169570"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37169570"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37169570; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37169570]").text(description); $(".js-view-count[data-work-id=37169570]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37169570; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37169570']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37169570, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7cfeec238ea3bb7cc084fc42fed9af40" } } $('.js-work-strip[data-work-id=37169570]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37169570,"title":"Modifying astronauts' microbiome may enhance prevention from commensal bacteria caused infections at microgravity condition","translated_title":"","metadata":{"doi":"10.5281/zenodo.15686","abstract":"Since in the 2020s, astronauts will be expected to build a colony at Mars without return. 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Biological System Design Tool" class="work-thumbnail" src="https://attachments.academia-assets.com/38485954/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/14950138/BioGuide_Biological_System_Design_Tool">BioGuide: Biological System Design Tool</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://etu.academia.edu/MAGCA">Muhammed Akif AGCA</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://metu.academia.edu/TolgaCan">Tolga Can</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://ku.academia.edu/HassanMatar">Hassan Matar</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">BioGuide is the first designed software that organizes over 1000 parts in parts.igem.org as possi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">BioGuide is the first designed software that organizes over 1000 parts in parts.igem.org as possible atomics parts to build new biological device and systems for specific input and outputs based on graph theory.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a29f0bc0ca4e693efabf8bba292919a8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" 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Biology","url":"https://www.academia.edu/Documents/in/Synthetic_Biology"},{"id":5026,"name":"Genetic Algorithms","url":"https://www.academia.edu/Documents/in/Genetic_Algorithms"},{"id":1317092,"name":"IGEM","url":"https://www.academia.edu/Documents/in/IGEM"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="profile--tab_heading_container js-section-heading" data-section="Röportaj & Söyleşi" id="Röportaj & Söyleşi"><h3 class="profile--tab_heading_container">Röportaj & Söyleşi by Cihan Tastan</h3></div><div class="js-work-strip profile--work_container" data-work-id="42688329"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/42688329/Ba%C5%9Flang%C4%B1%C3%A7_Noktas%C4%B1_Covid_19_hakk%C4%B1nda_sanal_bir_s%C3%B6yle%C5%9Fi"><img alt="Research paper thumbnail of Başlangıç Noktası: Covid-19 hakkında sanal bir söyleşi" class="work-thumbnail" src="https://attachments.academia-assets.com/62898795/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/42688329/Ba%C5%9Flang%C4%B1%C3%A7_Noktas%C4%B1_Covid_19_hakk%C4%B1nda_sanal_bir_s%C3%B6yle%C5%9Fi">Başlangıç Noktası: Covid-19 hakkında sanal bir söyleşi</a></div><div class="wp-workCard_item"><span>Başlangıç Noktası</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">COVID-19 salgını, bence dünyayı hızla milenyum çağına zorlamış durumda. Sadece 4-5 aylık bir salg...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">COVID-19 salgını, bence dünyayı hızla milenyum çağına zorlamış durumda. Sadece 4-5 aylık bir salgın olmasına karşın en az 670 bilimsel yayın yayımlanmış ve 120’den fazla klinik deneme başvurusu <br />yapılmış durumda. Bu şimdiye kadar gördüğüm en hızlı bilimsel savaş. Ve kısa zamanda COVID-19’un üstesinden  geleceğimizin  habercisi.  Bilim  dünyasında  gerçekleşen  bu  muazzam  hız,  normal hayatımızda kullandığımız teknolojileri de bir anda çağ atlattı. Artık siparişlerimiz drone’larveya otonom araçlarla ile daha sık getirilmeye dünyada başladı bile. Günlük hayatımızda kullandığımız GPS, Güney Kore’de hastalık kapmış insanların olduğu bölgeleri anlık canlı takip edebilecek şekilde sağlıklı insanları uyarmak için kullanılabiliyor.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f0f05092bf0677399628300219e849d5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":62898795,"asset_id":42688329,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/62898795/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="42688329"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="42688329"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42688329; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42688329]").text(description); $(".js-view-count[data-work-id=42688329]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 42688329; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='42688329']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 42688329, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f0f05092bf0677399628300219e849d5" } } $('.js-work-strip[data-work-id=42688329]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":42688329,"title":"Başlangıç Noktası: Covid-19 hakkında sanal bir söyleşi","translated_title":"","metadata":{"abstract":"COVID-19 salgını, bence dünyayı hızla milenyum çağına zorlamış durumda. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="3388452" id="scientificpapers"><div class="js-work-strip profile--work_container" data-work-id="50712322"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/50712322/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice"><img alt="Research paper thumbnail of Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice" class="work-thumbnail" src="https://attachments.academia-assets.com/68589782/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/50712322/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice">Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice</a></div><div class="wp-workCard_item"><span>Scientific Reports</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development fo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization for prolonged periods. In this study, a gamma-irradiated inactivated virus vaccine does not require an extra purification process, unlike the chemically inactivated vaccines. Hence, the novelty of our vaccine candidate (OZG-38.61.3) is that it is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. Efficiency and safety dose (either 10 13 or 10 14 viral RNA copy per dose) of OZG-38.61.3 was initially determined in BALB/c mice. This was followed by testing the immunogenicity and protective efficacy of the vaccine. Human ACE2-encoding transgenic mice were immunized and then infected with the SARS-CoV-2 virus for the challenge test. This study shows that vaccinated mice have lowered SARS-CoV-2 viral RNA copy numbers both in oropharyngeal specimens and in the histological analysis of the lung tissues along with humoral and cellular immune responses, including the neutralizing antibodies similar to those shown in BALB/c mice without substantial toxicity. Subsequently, plans are being</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c4dd6a30cdb04ebb0971fe7825484c44" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":68589782,"asset_id":50712322,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/68589782/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50712322"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50712322"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50712322; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50712322]").text(description); $(".js-view-count[data-work-id=50712322]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50712322; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='50712322']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 50712322, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c4dd6a30cdb04ebb0971fe7825484c44" } } $('.js-work-strip[data-work-id=50712322]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":50712322,"title":"Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice","translated_title":"","metadata":{"doi":"10.1038/s41598-021-95086-4","abstract":"The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization for prolonged periods. In this study, a gamma-irradiated inactivated virus vaccine does not require an extra purification process, unlike the chemically inactivated vaccines. Hence, the novelty of our vaccine candidate (OZG-38.61.3) is that it is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. Efficiency and safety dose (either 10 13 or 10 14 viral RNA copy per dose) of OZG-38.61.3 was initially determined in BALB/c mice. This was followed by testing the immunogenicity and protective efficacy of the vaccine. Human ACE2-encoding transgenic mice were immunized and then infected with the SARS-CoV-2 virus for the challenge test. This study shows that vaccinated mice have lowered SARS-CoV-2 viral RNA copy numbers both in oropharyngeal specimens and in the histological analysis of the lung tissues along with humoral and cellular immune responses, including the neutralizing antibodies similar to those shown in BALB/c mice without substantial toxicity. Subsequently, plans are being","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"Scientific Reports"},"translated_abstract":"The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization for prolonged periods. In this study, a gamma-irradiated inactivated virus vaccine does not require an extra purification process, unlike the chemically inactivated vaccines. 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COVID-19","url":"https://www.academia.edu/Documents/in/Coronavirus_COVID-19"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="49069265"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/49069265/The_Overall_Consequence_of_Antiviral_Drugs_Given_to_Pregnant_Women_with_COVID_19"><img alt="Research paper thumbnail of The Overall Consequence of Antiviral Drugs Given to Pregnant Women with COVID-19" class="work-thumbnail" src="https://attachments.academia-assets.com/67460627/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/49069265/The_Overall_Consequence_of_Antiviral_Drugs_Given_to_Pregnant_Women_with_COVID_19">The Overall Consequence of Antiviral Drugs Given to Pregnant Women with COVID-19</a></div><div class="wp-workCard_item"><span>Journal of Bacteriology & Parasitology</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">During pregnancy, the anatomical structure of the respiratory system changes, and the virus trans...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">During pregnancy, the anatomical structure of the respiratory system changes, and the virus transmitted by droplets and aerosols are more easily inhaled and difficult to remove by pregnant women. Women are generally more susceptible to various pregnancy-related complications and respiratory pathogens, increasing the risk of developing adverse pregnancy and neonatal outcomes. Anecdotal evidence suggests that pregnant women do not appear to differ from the general population in terms of disease transmission, and to date, there is no evidence of vertical transmission from mother to fetus. However, in another study, it is known that members of the coronavirus family are responsible for serious complications such as miscarriage, fetal growth restriction, and congenital anomalies during pregnancy. To date, only a few studies have reported relatively higher rates of adverse birth outcomes in women affected by SARS-CoV-2 infection in late pregnancy. This literature review presents the use of drugs used for the treatment of COVID-19 disease in pregnancy and pregnancy outcomes. It is also aimed to examine the effective control and management of SARS CoV-2 infection in the pregnancy, delivery, and postpartum period in line with the existing literature and guide health personals. Large-scale epidemiological studies are needed to evaluate the course of the infection during pregnancy and the effects of the drugs used on pregnancy and fetus.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a4c88f1d31633988bf58c6466520dab4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":67460627,"asset_id":49069265,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/67460627/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="49069265"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="49069265"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 49069265; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=49069265]").text(description); $(".js-view-count[data-work-id=49069265]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 49069265; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='49069265']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 49069265, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a4c88f1d31633988bf58c6466520dab4" } } $('.js-work-strip[data-work-id=49069265]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":49069265,"title":"The Overall Consequence of Antiviral Drugs Given to Pregnant Women with COVID-19","translated_title":"","metadata":{"abstract":"During pregnancy, the anatomical structure of the respiratory system changes, and the virus transmitted by droplets and aerosols are more easily inhaled and difficult to remove by pregnant women. Women are generally more susceptible to various pregnancy-related complications and respiratory pathogens, increasing the risk of developing adverse pregnancy and neonatal outcomes. Anecdotal evidence suggests that pregnant women do not appear to differ from the general population in terms of disease transmission, and to date, there is no evidence of vertical transmission from mother to fetus. However, in another study, it is known that members of the coronavirus family are responsible for serious complications such as miscarriage, fetal growth restriction, and congenital anomalies during pregnancy. To date, only a few studies have reported relatively higher rates of adverse birth outcomes in women affected by SARS-CoV-2 infection in late pregnancy. This literature review presents the use of drugs used for the treatment of COVID-19 disease in pregnancy and pregnancy outcomes. It is also aimed to examine the effective control and management of SARS CoV-2 infection in the pregnancy, delivery, and postpartum period in line with the existing literature and guide health personals. Large-scale epidemiological studies are needed to evaluate the course of the infection during pregnancy and the effects of the drugs used on pregnancy and fetus.","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"Journal of Bacteriology \u0026 Parasitology"},"translated_abstract":"During pregnancy, the anatomical structure of the respiratory system changes, and the virus transmitted by droplets and aerosols are more easily inhaled and difficult to remove by pregnant women. Women are generally more susceptible to various pregnancy-related complications and respiratory pathogens, increasing the risk of developing adverse pregnancy and neonatal outcomes. Anecdotal evidence suggests that pregnant women do not appear to differ from the general population in terms of disease transmission, and to date, there is no evidence of vertical transmission from mother to fetus. However, in another study, it is known that members of the coronavirus family are responsible for serious complications such as miscarriage, fetal growth restriction, and congenital anomalies during pregnancy. To date, only a few studies have reported relatively higher rates of adverse birth outcomes in women affected by SARS-CoV-2 infection in late pregnancy. This literature review presents the use of drugs used for the treatment of COVID-19 disease in pregnancy and pregnancy outcomes. It is also aimed to examine the effective control and management of SARS CoV-2 infection in the pregnancy, delivery, and postpartum period in line with the existing literature and guide health personals. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="45488617"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/45488617/Preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates"><img alt="Research paper thumbnail of Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates" class="work-thumbnail" src="https://attachments.academia-assets.com/65985763/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/45488617/Preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates">Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates</a></div><div class="wp-workCard_item"><span>Scientific Reports</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no v...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, SARS-CoV-2 vaccines have become crucial for reducing morbidity and mortality. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. The candidate vaccines in this study were OZG-3861 version 1 (V1), an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1), a GM-CSF adjuvant added vaccine. The candidate vaccines were applied intradermally to BALB/c mice to assess toxicity and immunogenicity. Preliminary results in vaccinated mice are reported in this study. Especially, the vaccine models containing GM-CSF caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature, when considered in terms of T and B cell responses. Another important finding was that the presence of adjuvant was more important in T cell in comparison with B cell response. Vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study shows that the vaccines are effective and leads us to start the challenge test to investigate the gamma-irradiated inactivated vaccine candidates for infective SARS-CoV-2 virus in humanized ACE2 + mice.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8b669e506ed29860174b2156e08ffda1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":65985763,"asset_id":45488617,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/65985763/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="45488617"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="45488617"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 45488617; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=45488617]").text(description); $(".js-view-count[data-work-id=45488617]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 45488617; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='45488617']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 45488617, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8b669e506ed29860174b2156e08ffda1" } } $('.js-work-strip[data-work-id=45488617]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":45488617,"title":"Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates","translated_title":"","metadata":{"doi":"10.1038/s41598-021-83930-6","abstract":"COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. 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wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/44803738/CRISPR_Of_Things_Applications_and_Challenges_of_the_Most_Popular_Gene_Editing_Tool_in_the_Fields_of_Health_Agriculture_and_Environment">CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://akdeniz.academia.edu/KenanTurgut">Kenan Turgut</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://atauni.academia.edu/KamilHAL%C4%B0LO%C4%9ELU">Kamil HALİLOĞLU</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://adnanmenderes.academia.edu/KemalBenlioglu">Kemal Benlioglu</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://comu.academia.edu/KemalMelihTASKIN">Kemal Melih TASKIN</a></span></div><div class="wp-workCard_item"><span>International Journal of Innovative Approaches in Agricultural Research</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Almost all cells of any living organism contain DNA, a hereditary molecule that passes from gener...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Almost all cells of any living organism contain DNA, a hereditary molecule that passes from generation to generation during reproduction. The term "genome" generally refers to the total DNA sequences in an organism. The genome consists of DNA sequences called "gene", which plays a role in the basic biological processes involved in many phenotypic and genotypic characteristics, such as performing cellular functions, controlling numbers and species, regulating energy production, metabolism, and combating diseases. Gene editing is the process of pre-designing and modifying a particular DNA sequence in a targeted gene. The most widely used technique is CRISPR-Cas technology. For this purpose, the DNA helix is cut at a certain point, to form a double-strand break (DSB), and naturally existing cellular repair mechanisms repair the DSB. Modes of the repair mechanisms may affect the gene function. When DSB is formed, gene editing techniques can be applied to remove, insert, or replace a newly modified sequence using a synthetic donor template DNA. In developed and developing countries, CRISPR-Cas studies in addition to research and development studies are rapidly increasing. In addition to increasing population, changing weather conditions, declining farmland, increasing biotic and abiotic stresses are other important barriers to agricultural production, food, and feed supply. In this report, CRISPR-Cas applications are introduced in detail from the studies that carried out gene modifications in the fields of health, animals, plants, microorganisms, and food supply. Besides, these technologies and applications have been examined in terms of world biosafety legislation and the scientific risk assessment of the products developed using the CRISPR-Cas technique.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="463a049aa56c9be6b61c75819c680172" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":65301681,"asset_id":44803738,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/65301681/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44803738"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44803738"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44803738; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44803738]").text(description); $(".js-view-count[data-work-id=44803738]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44803738; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44803738']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44803738, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "463a049aa56c9be6b61c75819c680172" } } $('.js-work-strip[data-work-id=44803738]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44803738,"title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment","translated_title":"","metadata":{"doi":"10.29329/ijiasr.2020.312.6","abstract":"Almost all cells of any living organism contain DNA, a hereditary molecule that passes from generation to generation during reproduction. 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In developed and developing countries, CRISPR-Cas studies in addition to research and development studies are rapidly increasing. In addition to increasing population, changing weather conditions, declining farmland, increasing biotic and abiotic stresses are other important barriers to agricultural production, food, and feed supply. In this report, CRISPR-Cas applications are introduced in detail from the studies that carried out gene modifications in the fields of health, animals, plants, microorganisms, and food supply. 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Gene editing is the process of pre-designing and modifying a particular DNA sequence in a targeted gene. The most widely used technique is CRISPR-Cas technology. For this purpose, the DNA helix is cut at a certain point, to form a double-strand break (DSB), and naturally existing cellular repair mechanisms repair the DSB. Modes of the repair mechanisms may affect the gene function. When DSB is formed, gene editing techniques can be applied to remove, insert, or replace a newly modified sequence using a synthetic donor template DNA. In developed and developing countries, CRISPR-Cas studies in addition to research and development studies are rapidly increasing. In addition to increasing population, changing weather conditions, declining farmland, increasing biotic and abiotic stresses are other important barriers to agricultural production, food, and feed supply. In this report, CRISPR-Cas applications are introduced in detail from the studies that carried out gene modifications in the fields of health, animals, plants, microorganisms, and food supply. Besides, these technologies and applications have been examined in terms of world biosafety legislation and the scientific risk assessment of the products developed using the CRISPR-Cas technique.","internal_url":"https://www.academia.edu/44803738/CRISPR_Of_Things_Applications_and_Challenges_of_the_Most_Popular_Gene_Editing_Tool_in_the_Fields_of_Health_Agriculture_and_Environment","translated_internal_url":"","created_at":"2020-12-30T14:47:08.767-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[{"id":36106908,"work_id":44803738,"tagging_user_id":20976874,"tagged_user_id":46883327,"co_author_invite_id":null,"email":"k***t@akdeniz.edu.tr","affiliation":"Akdeniz University","display_order":2,"name":"Kenan Turgut","title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment"},{"id":36106909,"work_id":44803738,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":7168523,"email":"c***k@iku.edu.tr","display_order":3,"name":"Cimen Atak","title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment"},{"id":36106910,"work_id":44803738,"tagging_user_id":20976874,"tagged_user_id":6021504,"co_author_invite_id":null,"email":"k***l@haliloglu.org","affiliation":"Ataturk University","display_order":4,"name":"Kamil HALİLOĞLU","title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment"},{"id":36106911,"work_id":44803738,"tagging_user_id":20976874,"tagged_user_id":46589724,"co_author_invite_id":null,"email":"k***u@adu.edu.tr","affiliation":"Adnan Menderes University","display_order":5,"name":"Kemal Benlioglu","title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment"},{"id":36106912,"work_id":44803738,"tagging_user_id":20976874,"tagged_user_id":46932606,"co_author_invite_id":null,"email":"k***n@comu.edu.tr","display_order":6,"name":"Kemal Taşkin","title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment"},{"id":36106913,"work_id":44803738,"tagging_user_id":20976874,"tagged_user_id":226001621,"co_author_invite_id":5126937,"email":"b***s@hacettepe.edu.tr","display_order":7,"name":"NURHAYAT BARLAS","title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment"},{"id":36106914,"work_id":44803738,"tagging_user_id":20976874,"tagged_user_id":47323558,"co_author_invite_id":null,"email":"k***n@gmail.com","affiliation":"Canakkale Onsekiz Mart University","display_order":8,"name":"Kemal Melih TASKIN","title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment"},{"id":36108001,"work_id":44803738,"tagging_user_id":6021504,"tagged_user_id":null,"co_author_invite_id":7168771,"email":"g***z@ankara.edu.tr","display_order":4194308,"name":"\u0026Amp; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="44701276"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/44701276/Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics"><img alt="Research paper thumbnail of Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics" class="work-thumbnail" src="https://attachments.academia-assets.com/65178708/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/44701276/Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics">Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/berberburak">burak berber</a></span></div><div class="wp-workCard_item"><span>Gene Therapy</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease cause...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV-2) (Prec Clin Med 2020;3:9-13, Lancet 2020;395:497-506, N. Engl J Med 2020a;382:1199-207, Nature 2020;579:270-3). SARS-CoV-2 surveillance is essential to controlling widespread transmission. However, there are several challenges associated with the diagnostic of the COVID-19 during the current outbreak (Liu and Li (2019), Nature 2020;579:265-9, N. Engl J Med 2020;382:727-33). Firstly, the high number of cases overwhelms diagnostic test capacity and proposes the need for a rapid solution for sample processing (Science 2018;360:444-8). Secondly, SARS-CoV-2 is closely related to other important coronavirus species and subspecies, so detection assays can give false-positive results if they are not efficiently specific to SARS-CoV-2. Thirdly, patients with suspected SARS-CoV-2 infection sometimes have a different respiratory viral infection or co-infections with SARS-CoV-2 and other respiratory viruses (MedRxiv 2020a;1-18). Confirmation of the COVID-19 is performed mainly by virus isolation followed by RT-PCR and sequencing (N. Engl J Med 2020;382:727-33, MedRxiv 2020a, Turkish J Biol 2020;44:192-202). The emergence and outbreak of the novel coronavirus highlighted the urgent need for new therapeutic technologies that are fast, precise, stable, easy to manufacture, and target-specific for surveillance and treatment. Molecular biology tools that include gene-editing approaches such as CRISPR-Cas12/13-based SHERLOCK, DETECTR, CARVER and PAC-MAN, antisense oligonucleotides, antisense peptide nucleic acids, ribozymes, aptamers, and RNAi silencing approaches produced with cutting-edge scientific advances compared to conventional diagnostic or treatment methods could be vital in COVID-19 and other future outbreaks. Thus, in this review, we will discuss potent the molecular biology approaches that can revolutionize diagnostic of viral infections and therapies to fight COVID-19 in a highly specific, stable, and efficient way. Highlights • SARS-CoV-2 is an RNA virus that could be targeted and diagnosed with novel CRISPR approaches. • ASO therapy targeting transcript encoding a viral protein or genomic RNA itself could be developed as a response to the SARS-CoV-2 pandemic.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c22858b6a747dd1fe395733e8dd6e21c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":65178708,"asset_id":44701276,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/65178708/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44701276"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44701276"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44701276; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44701276]").text(description); $(".js-view-count[data-work-id=44701276]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44701276; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44701276']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44701276, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c22858b6a747dd1fe395733e8dd6e21c" } } $('.js-work-strip[data-work-id=44701276]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44701276,"title":"Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics","translated_title":"","metadata":{"doi":"10.1038/s41434-020-00209-7","abstract":"The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV-2) (Prec Clin Med 2020;3:9-13, Lancet 2020;395:497-506, N. Engl J Med 2020a;382:1199-207, Nature 2020;579:270-3). SARS-CoV-2 surveillance is essential to controlling widespread transmission. However, there are several challenges associated with the diagnostic of the COVID-19 during the current outbreak (Liu and Li (2019), Nature 2020;579:265-9, N. Engl J Med 2020;382:727-33). Firstly, the high number of cases overwhelms diagnostic test capacity and proposes the need for a rapid solution for sample processing (Science 2018;360:444-8). Secondly, SARS-CoV-2 is closely related to other important coronavirus species and subspecies, so detection assays can give false-positive results if they are not efficiently specific to SARS-CoV-2. Thirdly, patients with suspected SARS-CoV-2 infection sometimes have a different respiratory viral infection or co-infections with SARS-CoV-2 and other respiratory viruses (MedRxiv 2020a;1-18). Confirmation of the COVID-19 is performed mainly by virus isolation followed by RT-PCR and sequencing (N. Engl J Med 2020;382:727-33, MedRxiv 2020a, Turkish J Biol 2020;44:192-202). The emergence and outbreak of the novel coronavirus highlighted the urgent need for new therapeutic technologies that are fast, precise, stable, easy to manufacture, and target-specific for surveillance and treatment. Molecular biology tools that include gene-editing approaches such as CRISPR-Cas12/13-based SHERLOCK, DETECTR, CARVER and PAC-MAN, antisense oligonucleotides, antisense peptide nucleic acids, ribozymes, aptamers, and RNAi silencing approaches produced with cutting-edge scientific advances compared to conventional diagnostic or treatment methods could be vital in COVID-19 and other future outbreaks. Thus, in this review, we will discuss potent the molecular biology approaches that can revolutionize diagnostic of viral infections and therapies to fight COVID-19 in a highly specific, stable, and efficient way. 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href="https://www.academia.edu/44393166/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice"><img alt="Research paper thumbnail of Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice" class="work-thumbnail" src="https://attachments.academia-assets.com/64799180/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/44393166/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice">Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/DeryaKan%C3%A7a%C4%9Fi">Derya Kançaği</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/GamzeTumentemur">Gamze Tumentemur</a></span></div><div class="wp-workCard_item"><span>BioRxiv </span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The SARS-CoV-2 virus caused one of the severest pandemic around the world. The vaccine developmen...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The SARS-CoV-2 virus caused one of the severest pandemic around the world. The vaccine development for urgent use became more of an issue during the pandemic. An inactivated virus formulated vaccines such as Hepatitis A, inactivated polio, and influenza has been proven to be a reliable approach for immunization for long years. In this pandemic, we produced an inactivated SARS-CoV-2 vaccine candidate by modification of the oldest but the most experienced method that can be produced quickly and tested easily rather than the recombinant vaccines. Here, we optimized an inactivated virus vaccine which includes the gamma irradiation process for the inactivation as an alternative to classical chemical inactivation methods so that there is no extra purification required. Also, we applied the vaccine candidate (OZG-38.61.3) using the intradermal route in mice which decreased the requirement of a higher concentration of inactivated virus for proper immunization unlike most of the classical inactivated vaccine treatments. Thus, the novelty of our vaccine candidate (OZG-38.61.3) is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. We first determined the efficiency and safety dose (either 1013 or 1014 viral copy per dose) of the OZG-38.61.3 in Balb/c mice. Next, to test the immunogenicity and protective efficacy of the OZG-38.61.3, we immunized human ACE2-encoding transgenic mice and infected them with a dose of infective SARS-CoV-2 virus for the challenge test. We showed that the vaccinated mice showed lowered SARS-CoV-2 viral copy number in oropharyngeal specimens along with humoral and cellular immune responses against the SARS-CoV-2, including the neutralizing antibodies similar to those shown in Balb/c mice without substantial toxicity. This study encouraged us towards a new promising strategy for inactivated vaccine development (OZG-38.61.3) and the Phase 1 clinical trial for the COVID-19 pandemic.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d4a40ec4c69425148f4e1407fb7125ad" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64799180,"asset_id":44393166,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64799180/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44393166"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44393166"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44393166; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44393166]").text(description); $(".js-view-count[data-work-id=44393166]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44393166; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44393166']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44393166, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d4a40ec4c69425148f4e1407fb7125ad" } } $('.js-work-strip[data-work-id=44393166]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44393166,"title":"Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice","translated_title":"","metadata":{"doi":"10.1101/2020.10.28.356667","abstract":"The SARS-CoV-2 virus caused one of the severest pandemic around the world. 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data-click-track="profile-work-strip-title" href="https://www.academia.edu/44037453/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection">Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection</a></div><div class="wp-workCard_item"><span>New Microbes and New Infections </span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). It is well known that novel Coronavirus disease 2019 (COVID-19) pneumonia progresses to ARDS and even multiple organ failure. High blood neutrophil levels are an early indicator of COVID-19 and predict severe respiratory diseases. Also it is reported that mucus structure of COVID-19 is very similar to cystic fibrosis due to the accumulation of excessive NET in the lungs. In this study, we showed the recovery of three COVID-19 patients after including Dornase alfa in their treatment. We followed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea, coughing and a decrease in NET formation and SARS-CoV-2 viral load after the treatment. Also here, we share our preliminary results suggesting that Dornase alfa has an anti-viral effect against SARS-CoV-2 infection in a green monkey kidney cell line, Vero, and a bovine kidney cell line, MDBK without determined cytotoxicity on healthy peripheral blood mononuclear cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c4f9f71592db803d5d74dcfe119443d0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64528192,"asset_id":44037453,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64528192/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44037453"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44037453"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44037453; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44037453]").text(description); $(".js-view-count[data-work-id=44037453]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44037453; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44037453']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44037453, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c4f9f71592db803d5d74dcfe119443d0" } } $('.js-work-strip[data-work-id=44037453]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44037453,"title":"Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection","translated_title":"","metadata":{"doi":"10.1016/j.nmni.2020.100756","abstract":"Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="44020845"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/44020845/Preliminary_report_of_preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates_SK_01_version_1_and_OZG_3861_version_1"><img alt="Research paper thumbnail of Preliminary report of preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates, SK-01 version 1 and OZG-3861 version 1" class="work-thumbnail" src="https://attachments.academia-assets.com/64356882/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/44020845/Preliminary_report_of_preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates_SK_01_version_1_and_OZG_3861_version_1">Preliminary report of preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates, SK-01 version 1 and OZG-3861 version 1</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://istanbul.academia.edu/UgurOzbek">Ugur Ozbek</a></span></div><div class="wp-workCard_item"><span>BioRxiv </span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The COVID-19 outbreak caused by SARS-CoV-2 has created an unprecedented health crisis since there...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The COVID-19 outbreak caused by SARS-CoV-2 has created an unprecedented health crisis since there is no coronavirus vaccine in the market due to the novelty of this virus. Therefore, SARS-CoV-2 vaccines have become very important to reduce morbidity and mortality. At this point, inactivated vaccines are important because the straightforward process of existing infrastructure used for several licensed human vaccines can be used for SARS-CoV-2. Inactive vaccines provide an antigenic presentation similar to that when they encounter invasive virus particles of the immune system. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. Our candidate OZG-3861 version 1 (V1) is an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1) is the GM-CSF adjuvant added vaccine candidate. We applied the candidates intradermal to BALB/c mice to assess the toxicity and immunogenicity of the OZG-3861 V1 and SK-01 V1. Here, we report our preliminary results in vaccinated mice. When considered in terms of T and B cell responses, it was observed that especially the vaccine models containing GM-CSF as an adjuvant caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature. Another finding showed that the presence of adjuvant is more important in T cell response rather than B cell. The vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study encouraged us to start the challenge test using infective SARS-CoV-2 viruses and our second version of gamma-irradiated inactivated vaccine candidates in humanized ACE2+ mice.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f798e80c458a6360fd5c053083342415" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64356882,"asset_id":44020845,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64356882/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="44020845"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="44020845"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44020845; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44020845]").text(description); $(".js-view-count[data-work-id=44020845]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44020845; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='44020845']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 44020845, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f798e80c458a6360fd5c053083342415" } } $('.js-work-strip[data-work-id=44020845]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":44020845,"title":"Preliminary report of preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates, SK-01 version 1 and OZG-3861 version 1","translated_title":"","metadata":{"doi":"10.1101/2020.09.04.277426","abstract":"The COVID-19 outbreak caused by SARS-CoV-2 has created an unprecedented health crisis since there is no coronavirus vaccine in the market due to the novelty of this virus. Therefore, SARS-CoV-2 vaccines have become very important to reduce morbidity and mortality. At this point, inactivated vaccines are important because the straightforward process of existing infrastructure used for several licensed human vaccines can be used for SARS-CoV-2. Inactive vaccines provide an antigenic presentation similar to that when they encounter invasive virus particles of the immune system. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. Our candidate OZG-3861 version 1 (V1) is an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1) is the GM-CSF adjuvant added vaccine candidate. We applied the candidates intradermal to BALB/c mice to assess the toxicity and immunogenicity of the OZG-3861 V1 and SK-01 V1. Here, we report our preliminary results in vaccinated mice. When considered in terms of T and B cell responses, it was observed that especially the vaccine models containing GM-CSF as an adjuvant caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature. Another finding showed that the presence of adjuvant is more important in T cell response rather than B cell. The vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study encouraged us to start the challenge test using infective SARS-CoV-2 viruses and our second version of gamma-irradiated inactivated vaccine candidates in humanized ACE2+ mice.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"BioRxiv "},"translated_abstract":"The COVID-19 outbreak caused by SARS-CoV-2 has created an unprecedented health crisis since there is no coronavirus vaccine in the market due to the novelty of this virus. Therefore, SARS-CoV-2 vaccines have become very important to reduce morbidity and mortality. At this point, inactivated vaccines are important because the straightforward process of existing infrastructure used for several licensed human vaccines can be used for SARS-CoV-2. Inactive vaccines provide an antigenic presentation similar to that when they encounter invasive virus particles of the immune system. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. Our candidate OZG-3861 version 1 (V1) is an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1) is the GM-CSF adjuvant added vaccine candidate. We applied the candidates intradermal to BALB/c mice to assess the toxicity and immunogenicity of the OZG-3861 V1 and SK-01 V1. Here, we report our preliminary results in vaccinated mice. When considered in terms of T and B cell responses, it was observed that especially the vaccine models containing GM-CSF as an adjuvant caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature. Another finding showed that the presence of adjuvant is more important in T cell response rather than B cell. The vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study encouraged us to start the challenge test using infective SARS-CoV-2 viruses and our second version of gamma-irradiated inactivated vaccine candidates in humanized ACE2+ mice.","internal_url":"https://www.academia.edu/44020845/Preliminary_report_of_preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates_SK_01_version_1_and_OZG_3861_version_1","translated_internal_url":"","created_at":"2020-09-04T20:43:28.149-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[{"id":35672038,"work_id":44020845,"tagging_user_id":20976874,"tagged_user_id":60081917,"co_author_invite_id":null,"email":"g***r@gmail.com","display_order":1,"name":"Gözde Sır","title":"Preliminary report of preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates, SK-01 version 1 and OZG-3861 version 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Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients" class="work-thumbnail" src="https://attachments.academia-assets.com/64983046/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/43822999/Preclinical_Assessment_of_Efficacy_and_Safety_Analysis_of_CAR_T_Cells_ISIKOK_19_targeting_CD19_expressing_B_Cells_for_the_First_Turkish_Academic_Clinical_Trial_with_Relapsed_refractory_ALL_and_NHL_Patients">Preclinical Assessment of Efficacy and Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Didem%C3%87ak%C4%B1rsoy">Didem Çakırsoy</a></span></div><div class="wp-workCard_item"><span>Turkish Journal of Hematology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Relapsed and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin L...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Relapsed and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin Lymphoma (NHL) are the focus of studies in hematological cancers. Treatment of these malignancies has undergone recent transformation with the development of new gene therapy and molecular biology techniques, which are safer and well-tolerated therapeutic approaches. The CD19 antigen is the most studied therapeutic target in these hematological cancers. For this purpose, we used a lentiviral vector encoding the CD19 antigen-specific antibody head (FMC63) conjugated with CD8-CD28-CD3ζ sequence as a chimeric antigen receptor (CAR) along with a truncated form of EGFR (EGFRt) on human T lymphocytes (CAR-T). In this study, we pre-clinically assessed efficacy and safety of the manufactured CAR-T cells both from healthy donor and ALL/NHL patient’ peripheral blood mononuclear cells, namely ISIKOK-19. Furthermore, we showed significant enhancement of CAR lentivirus transduction efficacy in T cells using BX-795, an inhibitor of the signaling molecule TBK1/IKKƐ in order to cut the cost of CAR-T cell production. In addition, ISIKOK-19 cells demonstrated a significant high level of cytotoxicity specifically against a CD19+ B-lymphocyte cancer model, RAJI cells, in NOD/SCID mice. This is the first report of preclinical assessment of efficacy and safety analysis of CAR-T cells (ISIKOK-19) targeting CD19-expressing B cells in relapsed/refractory ALL and NHL patients in Turkey.  Keywords: Chimeric Antigen Receptor, CD19, CAR-T, Immunotherapy Preclinical Assessment of Efficacy and Safety Analysis of CAR-T cells (ISIKOK-19) targeting CD19-expressing B cells for the first Turkish academic clinical trial with relapsed/refractory ALL and NHL patients</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a631f9a5ae85041038bb5c6d9c88898b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64983046,"asset_id":43822999,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64983046/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="43822999"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="43822999"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 43822999; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=43822999]").text(description); $(".js-view-count[data-work-id=43822999]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 43822999; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='43822999']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 43822999, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a631f9a5ae85041038bb5c6d9c88898b" } } $('.js-work-strip[data-work-id=43822999]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":43822999,"title":"Preclinical Assessment of Efficacy and Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients","translated_title":"","metadata":{"doi":"10.4274/tjh.galenos.2020.2020.0070","abstract":"Relapsed and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin Lymphoma (NHL) are the focus of studies in hematological cancers. Treatment of these malignancies has undergone recent transformation with the development of new gene therapy and molecular biology techniques, which are safer and well-tolerated therapeutic approaches. The CD19 antigen is the most studied therapeutic target in these hematological cancers. For this purpose, we used a lentiviral vector encoding the CD19 antigen-specific antibody head (FMC63) conjugated with CD8-CD28-CD3ζ sequence as a chimeric antigen receptor (CAR) along with a truncated form of EGFR (EGFRt) on human T lymphocytes (CAR-T). In this study, we pre-clinically assessed efficacy and safety of the manufactured CAR-T cells both from healthy donor and ALL/NHL patient’ peripheral blood mononuclear cells, namely ISIKOK-19. Furthermore, we showed significant enhancement of CAR lentivirus transduction efficacy in T cells using BX-795, an inhibitor of the signaling molecule TBK1/IKKƐ in order to cut the cost of CAR-T cell production. In addition, ISIKOK-19 cells demonstrated a significant high level of cytotoxicity specifically against a CD19+ B-lymphocyte cancer model, RAJI cells, in NOD/SCID mice. This is the first report of preclinical assessment of efficacy and safety analysis of CAR-T cells (ISIKOK-19) targeting CD19-expressing B cells in relapsed/refractory ALL and NHL patients in Turkey. Keywords: Chimeric Antigen Receptor, CD19, CAR-T, Immunotherapy Preclinical Assessment of Efficacy and Safety Analysis of CAR-T cells (ISIKOK-19) targeting CD19-expressing B cells for the first Turkish academic clinical trial with relapsed/refractory ALL and NHL patients","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Turkish Journal of Hematology"},"translated_abstract":"Relapsed and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin Lymphoma (NHL) are the focus of studies in hematological cancers. Treatment of these malignancies has undergone recent transformation with the development of new gene therapy and molecular biology techniques, which are safer and well-tolerated therapeutic approaches. The CD19 antigen is the most studied therapeutic target in these hematological cancers. For this purpose, we used a lentiviral vector encoding the CD19 antigen-specific antibody head (FMC63) conjugated with CD8-CD28-CD3ζ sequence as a chimeric antigen receptor (CAR) along with a truncated form of EGFR (EGFRt) on human T lymphocytes (CAR-T). In this study, we pre-clinically assessed efficacy and safety of the manufactured CAR-T cells both from healthy donor and ALL/NHL patient’ peripheral blood mononuclear cells, namely ISIKOK-19. Furthermore, we showed significant enhancement of CAR lentivirus transduction efficacy in T cells using BX-795, an inhibitor of the signaling molecule TBK1/IKKƐ in order to cut the cost of CAR-T cell production. In addition, ISIKOK-19 cells demonstrated a significant high level of cytotoxicity specifically against a CD19+ B-lymphocyte cancer model, RAJI cells, in NOD/SCID mice. This is the first report of preclinical assessment of efficacy and safety analysis of CAR-T cells (ISIKOK-19) targeting CD19-expressing B cells in relapsed/refractory ALL and NHL patients in Turkey. 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Immunotherapy","url":"https://www.academia.edu/Documents/in/Cancer_Immunotherapy"},{"id":126863,"name":"Immunotherapy","url":"https://www.academia.edu/Documents/in/Immunotherapy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="43399947"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/43399947/SARS_CoV_2_isolation_and_propagation_from_Turkish_COVID_19_patients"><img alt="Research paper thumbnail of SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients" class="work-thumbnail" src="https://attachments.academia-assets.com/63697379/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/43399947/SARS_CoV_2_isolation_and_propagation_from_Turkish_COVID_19_patients">SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SelenAbanuz">Selen Abanuz</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KorayYal%C3%A7%C4%B1n">Koray Yalçın</a></span></div><div class="wp-workCard_item"><span>Turkish Journal of Biology </span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6749ad56d35302e4e11058893da5e774" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":63697379,"asset_id":43399947,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/63697379/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="43399947"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="43399947"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 43399947; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=43399947]").text(description); $(".js-view-count[data-work-id=43399947]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 43399947; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='43399947']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 43399947, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6749ad56d35302e4e11058893da5e774" } } $('.js-work-strip[data-work-id=43399947]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":43399947,"title":"SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients","translated_title":"","metadata":{"doi":"10.3906/biy-2004-113","abstract":"The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Turkish Journal of Biology "},"translated_abstract":"The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="43041910"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/43041910/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection"><img alt="Research paper thumbnail of Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection" class="work-thumbnail" src="https://attachments.academia-assets.com/63302019/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/43041910/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection">Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection</a></div><div class="wp-workCard_item"><span>JMIR Preprints</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). Coronavirus disease 2019(COVID-19) pandemic affected more than two million people over the world, resulting in unprecedented health, social and economic crises. The COVID-19, viral pneumonia that progresses to ARDS and even multiple organ failure, is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High blood neutrophil levels are an early indicator of SARS-CoV-2infection and predict severe respiratory diseases. A similar mucus structure is detected in COVID-19patients due to the accumulation of excessive NET in the lungs. Here, we show our preliminary results with dornase alfa that may have an in-vitro anti-viral effect against SARS-CoV-2 infection ina bovine kidney cell line, MDBK without drug toxicity on healthy adult peripheral blood mononuclear cells. In this preliminary study, we also showed that dornase alfa can promote clearance of NET formation in both an in-vitro and three COVID-19 cases who showed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea and coughing.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="172e5e8f5bbac46c06b73a7d42f36ba2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":63302019,"asset_id":43041910,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/63302019/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="43041910"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="43041910"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 43041910; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=43041910]").text(description); $(".js-view-count[data-work-id=43041910]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 43041910; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='43041910']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 43041910, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "172e5e8f5bbac46c06b73a7d42f36ba2" } } $('.js-work-strip[data-work-id=43041910]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":43041910,"title":"Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection","translated_title":"","metadata":{"doi":"10.2196/preprints.20200","abstract":"Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). Coronavirus disease 2019(COVID-19) pandemic affected more than two million people over the world, resulting in unprecedented health, social and economic crises. The COVID-19, viral pneumonia that progresses to ARDS and even multiple organ failure, is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High blood neutrophil levels are an early indicator of SARS-CoV-2infection and predict severe respiratory diseases. A similar mucus structure is detected in COVID-19patients due to the accumulation of excessive NET in the lungs. Here, we show our preliminary results with dornase alfa that may have an in-vitro anti-viral effect against SARS-CoV-2 infection ina bovine kidney cell line, MDBK without drug toxicity on healthy adult peripheral blood mononuclear cells. In this preliminary study, we also showed that dornase alfa can promote clearance of NET formation in both an in-vitro and three COVID-19 cases who showed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea and coughing.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"JMIR Preprints"},"translated_abstract":"Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. 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Here, we show our preliminary results with dornase alfa that may have an in-vitro anti-viral effect against SARS-CoV-2 infection ina bovine kidney cell line, MDBK without drug toxicity on healthy adult peripheral blood mononuclear cells. In this preliminary study, we also showed that dornase alfa can promote clearance of NET formation in both an in-vitro and three COVID-19 cases who showed clinical improvement in the radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea and coughing.","internal_url":"https://www.academia.edu/43041910/Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection","translated_internal_url":"","created_at":"2020-05-13T19:09:49.606-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"draft","co_author_tags":[],"downloadable_attachments":[{"id":63302019,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/63302019/thumbnails/1.jpg","file_name":"preprint-20200-submitted20200513-62035-8879ry.pdf","download_url":"https://www.academia.edu/attachments/63302019/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Preliminary_Report_of_In_vitro_and_In_vi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/63302019/preprint-20200-submitted20200513-62035-8879ry-libre.pdf?1589422926=\u0026response-content-disposition=attachment%3B+filename%3DPreliminary_Report_of_In_vitro_and_In_vi.pdf\u0026Expires=1732704101\u0026Signature=Ur8tDlTNMV2o5luKew2~kIcKrjYLLtVIUuQ0qW1Bf5Po-G55PxlNobgvdlu2Ct7diO~FVc3UyBewXu58~YjYzkbyiLL94VbkLiN-2tV8XsAIageOttFTNKE4gFzzjk1gSHihl3KptghQxVwlzejR6qu0ArheM4lUr-MfSIM3c6aWcY6STwzzp5t9ZYHpmtDAueBLYHx39Vd3XazcHcL5jANIyA3~ow~EAZHqfCO4ob4y0zKQtABFcu3YMiOC8eycjv1aKUN8yKZH7hwqqNmHTHYnjEvixjpwcNP7e1AXZB7K7c9Tb5O6zVUxqVae6p42CoghMcjfJTsjJLKD~RUbfw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Preliminary_Report_of_In_vitro_and_In_vivo_Effectiveness_of_Dornase_Alfa_on_SARS_CoV_2_Infection","translated_slug":"","page_count":29,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"Ylhrc2I2SWIyd0E2QTgyU0JsNXNUdUxzTXVFVXVqNXVUL0NzVzQrdFA0ZG1XNFVhV3FIbHRTVVVxb1EweWI5VC0tZm1XZUVYcURYVndJZEpURnk3WEEvZz09--90a359e66e9f7cba8ab57a242052446933950186"},"attachments":[{"id":63302019,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/63302019/thumbnails/1.jpg","file_name":"preprint-20200-submitted20200513-62035-8879ry.pdf","download_url":"https://www.academia.edu/attachments/63302019/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Preliminary_Report_of_In_vitro_and_In_vi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/63302019/preprint-20200-submitted20200513-62035-8879ry-libre.pdf?1589422926=\u0026response-content-disposition=attachment%3B+filename%3DPreliminary_Report_of_In_vitro_and_In_vi.pdf\u0026Expires=1732704101\u0026Signature=Ur8tDlTNMV2o5luKew2~kIcKrjYLLtVIUuQ0qW1Bf5Po-G55PxlNobgvdlu2Ct7diO~FVc3UyBewXu58~YjYzkbyiLL94VbkLiN-2tV8XsAIageOttFTNKE4gFzzjk1gSHihl3KptghQxVwlzejR6qu0ArheM4lUr-MfSIM3c6aWcY6STwzzp5t9ZYHpmtDAueBLYHx39Vd3XazcHcL5jANIyA3~ow~EAZHqfCO4ob4y0zKQtABFcu3YMiOC8eycjv1aKUN8yKZH7hwqqNmHTHYnjEvixjpwcNP7e1AXZB7K7c9Tb5O6zVUxqVae6p42CoghMcjfJTsjJLKD~RUbfw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="42845858"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/42845858/SARS_CoV_2_Isolation_and_Propagation_from_Turkish_COVID_19_patients"><img alt="Research paper thumbnail of SARS-CoV-2 Isolation and Propagation from Turkish COVID-19 patients" class="work-thumbnail" src="https://attachments.academia-assets.com/63081606/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/42845858/SARS_CoV_2_Isolation_and_Propagation_from_Turkish_COVID_19_patients">SARS-CoV-2 Isolation and Propagation from Turkish COVID-19 patients</a></div><div class="wp-workCard_item"><span>bioRxiv</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The novel coronavirus pneumonia, which was named later as Coronavirus Disease 2019 (COVID-19), is...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The novel coronavirus pneumonia, which was named later as Coronavirus Disease 2019 (COVID-19), is caused by the Severe Acute Respiratory Syndrome Coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. Today, over eight thousand cases in Turkey, and two million cases around the world have been declared. Due to the urgent need for vaccine studies and drug discoveries, isolation of the virus is crucial. Here, we report one of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study suggests replication methodology and cell culture tropism of the virus that will be available to the research communities.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="59c157b4b282a345fd0c9464bfa6a733" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":63081606,"asset_id":42845858,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/63081606/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="42845858"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="42845858"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42845858; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42845858]").text(description); $(".js-view-count[data-work-id=42845858]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 42845858; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='42845858']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 42845858, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "59c157b4b282a345fd0c9464bfa6a733" } } $('.js-work-strip[data-work-id=42845858]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":42845858,"title":"SARS-CoV-2 Isolation and Propagation from Turkish COVID-19 patients","translated_title":"","metadata":{"doi":"10.1101/2020.04.23.056309","abstract":"The novel coronavirus pneumonia, which was named later as Coronavirus Disease 2019 (COVID-19), is caused by the Severe Acute Respiratory Syndrome Coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. Today, over eight thousand cases in Turkey, and two million cases around the world have been declared. Due to the urgent need for vaccine studies and drug discoveries, isolation of the virus is crucial. Here, we report one of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study suggests replication methodology and cell culture tropism of the virus that will be available to the research communities.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"bioRxiv"},"translated_abstract":"The novel coronavirus pneumonia, which was named later as Coronavirus Disease 2019 (COVID-19), is caused by the Severe Acute Respiratory Syndrome Coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. Today, over eight thousand cases in Turkey, and two million cases around the world have been declared. Due to the urgent need for vaccine studies and drug discoveries, isolation of the virus is crucial. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="42666338"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/42666338/Alternative_Therapies_to_Antibiotics_CRISPR_Cas_antimicrobials"><img alt="Research paper thumbnail of Alternative Therapies to Antibiotics: CRISPR-Cas antimicrobials" class="work-thumbnail" src="https://attachments.academia-assets.com/62874038/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/42666338/Alternative_Therapies_to_Antibiotics_CRISPR_Cas_antimicrobials">Alternative Therapies to Antibiotics: CRISPR-Cas antimicrobials</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ege.academia.edu/ay%C5%9Feg%C3%BClate%C5%9F">Ayşegül Ateş</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SafakErmertcan">Safak Ermertcan</a></span></div><div class="wp-workCard_item"><span>Gene Editing</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antibiotics affect specific mechanisms of bacteria by targeting cellular pathways or f...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antibiotics  affect  specific  mechanisms  of  bacteria  by  targeting  cellular  pathways  or  functions  such  as  function  of  cell membrane, blockage of cell wall synthesis, protein or nucleic acid synthesis. They can’t selectively  kill targeted  pathogens  in the  mixed  microbial  population  with  these  mechanisms.  Antibiotics  cause  dysfunction  not  only  of  the  bacteria  that  cause infection but also of the beneficial microbiota members in the host. Currently, there is no specific antibiotic strategy targeting only virulent or antibiotic-resistant bacteria. Current antibiotic strategies aren’t specific; resistant bacteria allow spread of the resistance  genes  in  the  bacterial  population.  Recently,  new  molecular  techniques  have  been  introduced  to deal  with antimicrobial  resistance.  Researchers  could  knock  out  plasmid-mediated  antibiotic  resistance  genes  in  order  to  prevent  the spread of resistance. This review will discuss antibiotic resistance, CRISPR-Cas9 gene editing mechanism and its applications against bacteria itself, which will be an important method to prevent the clonal spread of resistant strains, providing a unique solution to the global problem.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1f5226fb2b20504eed2bc0021bbb70a0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":62874038,"asset_id":42666338,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/62874038/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="42666338"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="42666338"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42666338; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42666338]").text(description); $(".js-view-count[data-work-id=42666338]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 42666338; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='42666338']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 42666338, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1f5226fb2b20504eed2bc0021bbb70a0" } } $('.js-work-strip[data-work-id=42666338]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":42666338,"title":"Alternative Therapies to Antibiotics: CRISPR-Cas antimicrobials","translated_title":"","metadata":{"doi":"10.29228/genediting.41450","abstract":"Antibiotics affect specific mechanisms of bacteria by targeting cellular pathways or functions such as function of cell membrane, blockage of cell wall synthesis, protein or nucleic acid synthesis. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="37253761"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/37253761/Tuning_of_human_MAIT_cell_activation_by_commensal_bacteria_species_and_MR1_dependent_T_cell_presentation"><img alt="Research paper thumbnail of Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation" class="work-thumbnail" src="https://attachments.academia-assets.com/61328942/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37253761/Tuning_of_human_MAIT_cell_activation_by_commensal_bacteria_species_and_MR1_dependent_T_cell_presentation">Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ElizabethFleming11">Elizabeth Fleming</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AnitaVoigt">Anita Voigt</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JuliaOh5">Julia Oh</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/DeryaUnutmaz">Derya Unutmaz</a></span></div><div class="wp-workCard_item"><span>Mucosal Immunology </span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. However, it is unclear whether MAIT cells discriminate between many species of the human microbiota. To address this, we developed an in vitro functional assay through human T cells engineered for MAIT-TCRs (eMAIT-TCRs) stimulated by MR1-expressing antigen-presenting cells (APCs). We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded the riboflavin pathway were stimulatory for MAIT-TCRs. Most species that were high stimulators belonged to Bacteroidetes and Proteobacteria phyla, whereas low/non-stimulator species were primarily Actinobacteria or Firmicutes. Activation of MAIT cells by high-vs low-stimulating bacteria also correlated with the level of riboflavin they secreted or after bacterial infection of macrophages. Remarkably, we found that human T-cell subsets can also present riboflavin metabolites to MAIT cells in a MR1-restricted fashion. This T–T cell-mediated signaling also induced IFNγ, TNF and granzyme B from MAIT cells, albeit at lower level than professional APC. These findings suggest that MAIT cells can discriminate and categorize complex human microbiota through computation of TCR signals depending on antigen load and presenting cells, and fine-tune their functional responses.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e5201d8b9be6d3c94615f81e9b153ffa" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61328942,"asset_id":37253761,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61328942/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37253761"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37253761"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37253761; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37253761]").text(description); $(".js-view-count[data-work-id=37253761]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37253761; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37253761']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37253761, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e5201d8b9be6d3c94615f81e9b153ffa" } } $('.js-work-strip[data-work-id=37253761]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37253761,"title":"Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation","translated_title":"","metadata":{"doi":"10.1038/s41385-018-0072-x","abstract":"Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. However, it is unclear whether MAIT cells discriminate between many species of the human microbiota. To address this, we developed an in vitro functional assay through human T cells engineered for MAIT-TCRs (eMAIT-TCRs) stimulated by MR1-expressing antigen-presenting cells (APCs). We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded the riboflavin pathway were stimulatory for MAIT-TCRs. Most species that were high stimulators belonged to Bacteroidetes and Proteobacteria phyla, whereas low/non-stimulator species were primarily Actinobacteria or Firmicutes. Activation of MAIT cells by high-vs low-stimulating bacteria also correlated with the level of riboflavin they secreted or after bacterial infection of macrophages. Remarkably, we found that human T-cell subsets can also present riboflavin metabolites to MAIT cells in a MR1-restricted fashion. This T–T cell-mediated signaling also induced IFNγ, TNF and granzyme B from MAIT cells, albeit at lower level than professional APC. These findings suggest that MAIT cells can discriminate and categorize complex human microbiota through computation of TCR signals depending on antigen load and presenting cells, and fine-tune their functional responses.","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"Mucosal Immunology "},"translated_abstract":"Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. However, it is unclear whether MAIT cells discriminate between many species of the human microbiota. To address this, we developed an in vitro functional assay through human T cells engineered for MAIT-TCRs (eMAIT-TCRs) stimulated by MR1-expressing antigen-presenting cells (APCs). We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded the riboflavin pathway were stimulatory for MAIT-TCRs. Most species that were high stimulators belonged to Bacteroidetes and Proteobacteria phyla, whereas low/non-stimulator species were primarily Actinobacteria or Firmicutes. Activation of MAIT cells by high-vs low-stimulating bacteria also correlated with the level of riboflavin they secreted or after bacterial infection of macrophages. Remarkably, we found that human T-cell subsets can also present riboflavin metabolites to MAIT cells in a MR1-restricted fashion. This T–T cell-mediated signaling also induced IFNγ, TNF and granzyme B from MAIT cells, albeit at lower level than professional APC. These findings suggest that MAIT cells can discriminate and categorize complex human microbiota through computation of TCR signals depending on antigen load and presenting cells, and fine-tune their functional responses.","internal_url":"https://www.academia.edu/37253761/Tuning_of_human_MAIT_cell_activation_by_commensal_bacteria_species_and_MR1_dependent_T_cell_presentation","translated_internal_url":"","created_at":"2018-08-17T11:41:51.512-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[{"id":31806760,"work_id":37253761,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":6724591,"email":"e***n@jax.org","display_order":1,"name":"Ece Karhan","title":"Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell 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Microbiota","url":"https://www.academia.edu/Documents/in/Gut_Microbiota"},{"id":166587,"name":"Mucosal Immunology","url":"https://www.academia.edu/Documents/in/Mucosal_Immunology"},{"id":1916835,"name":"CRISPR-Cas9 system","url":"https://www.academia.edu/Documents/in/CRISPR-Cas9_system"}],"urls":[{"id":8567513,"url":"https://www.nature.com/articles/s41385-018-0072-x"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="37094832"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/37094832/Functional_Interrogation_of_Primary_Human_T_Cells_via_CRISPR_Genetic_Editing"><img alt="Research paper thumbnail of Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing" class="work-thumbnail" src="https://attachments.academia-assets.com/61328995/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37094832/Functional_Interrogation_of_Primary_Human_T_Cells_via_CRISPR_Genetic_Editing">Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LindseyPlacek">Lindsey Placek</a></span></div><div class="wp-workCard_item"><span>The Journal of Immunology</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Developing precise and efficient gene editing approaches using CRISPR in primary human T cell sub...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (CD4, CD45, CD95) could be simultaneously and stably deleted in naive, memory, effector, or regulatory T cell (Treg) subsets at very high efficiency. Additionally, nuclease-deficient Cas9, associated with a transcriptional activator or repressor, can downregulate or increase expression of genes in T cells. For example, expression of glycoprotein A repetitions predominant (GARP), a gene that is normally and exclusively expressed on activated Tregs, could be induced on non-Treg effector T cells by nuclease-deficient Cas9 fused to transcriptional activators. Further analysis determined that this approach could be used in mapping promoter sequences involved in gene transcription. Through this CRISPR/Cas9–mediated genetic editing we also demonstrated the feasibility of human T cell functional analysis in several examples: 1) CD95 deletion inhibited T cell apoptosis upon reactivation; 2) deletion of ORAI1, a Ca2+ release–activated channel, abolished Ca2+ influx and cytokine secretion, mimicking natural genetic mutations in immune-deficient patients; and 3) transcriptional activation of CD25 or CD127 expression enhanced cytokine signaling by IL-2 or IL-7, respectively. Taken together, application of the CRISPR toolbox to human T cell subsets has important implications for decoding the mechanisms of their functional outputs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="da1ab496a3de8cd2f2431c42397d6603" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61328995,"asset_id":37094832,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61328995/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37094832"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37094832"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37094832; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37094832]").text(description); $(".js-view-count[data-work-id=37094832]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37094832; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37094832']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37094832, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "da1ab496a3de8cd2f2431c42397d6603" } } $('.js-work-strip[data-work-id=37094832]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37094832,"title":"Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing","translated_title":"","metadata":{"doi":"10.4049/jimmunol.1701616","abstract":"Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. 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src="https://attachments.academia-assets.com/55277057/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/35416278/PhD_Dissertation_Thesis_Abstract_Tuning_of_Human_Mucosal_Associated_Invariant_T_MAIT_Cell_Function_through_Microbiota_Mediated_T_Cell_Receptor_Signals">PhD Dissertation/Thesis Abstract: Tuning of Human Mucosal Associated Invariant T (MAIT) Cell Function through Microbiota-Mediated T Cell Receptor Signals</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated"> Mucosal-associated invariant T (MAIT) cells are a subset of the T cell population defined by an ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mucosal-associated invariant T (MAIT) cells are a subset of the T cell population defined by an invariant T cell receptor (TCR) that is stimulated by bacterial metabolites. These cells preferentially migrate to mucosal tissues such as gut, liver and skin. MAIT cells are activated by metabolites from the riboflavin (Vitamin B2) biosynthetic pathway that are presented by MHC class 1-related (MR1) molecules expressed on numerous of cell types including antigen presenting cells (APCs) and epithelial cells. The V alpha segment of MAIT TCRs is invariant and composed of Vα7.2, whereas the V beta portion can be variable. MAIT cells are present at very low levels in neonatal blood but significantly increase in adults and show very high variance in their frequency among individuals. While there is evidence that several pathogenic bacteria can activate MAIT cells and that one of their functions is to kill cells with intracellular bacteria, it remains unknown how they discriminate among thousands of commensal bacteria that can also produce Vitamin B2 metabolites. Further, the role of the TCR variable beta region in antigen recognition is still unclear. In this thesis project, we hypothesized that MAIT cells can be stimulated by bacteria that inhabit human mucosal tissues, and that this microbiota-MAIT cell interaction is one of the driving forces in their expansion and variation in the human population. In support of this hypothesis, we observed a reduced proportion of MAIT cells in the blood of perinatally HIV-infected children, which correlated with other microbiota-associated parameters including Th17 cells and inflammatory markers of microbiota alterations. However, it is unclear whether MAIT cells can discriminate bacterial species that reside in the human microbiota, which can produce the riboflavin intermediates. To address this, we developed an in vitro functional assay using human T cells engineered for MAIT-specific TCRs (eMAIT-TCRs) stimulated by MR1-expressing B cell lines presenting the bacterial metabolites. We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded a riboflavin biosynthesis pathway were stimulatory for MAIT-TCRs. Most species that were high-stimulators belonged to Bacteroidetes and Proteobacteria phyla, although with significant variance, whereas low/non-stimulator species were either Actinobacteria or Firmicutes. Furthermore, we determined a wide range of intra-species variation in eMAIT-TCR stimulation capacities of Staphylococcus epidermidis , a skin commensal, which suggests that bacteria can modulate the production capacities of MAIT-TCR stimulatory antigens. Remarkably, we also discovered that human T cells not only express low-levels of MR1 but can also present bacterial metabolites to MAIT cells in an MR1-restricted fashion and trigger TCR signaling to induce cytokine secretion (IFNγ and TNFα), albeit at lower levels. In conclusion, our findings revealed that MAIT cells could discriminate between MR1-restricted, bacteria-derived metabolites through their TCR signaling thresholds. This knowledge paves the way to elucidate the complexity of MAIT cell recognition and its response to the human microbiota, which establishes a framework to explore mechanistic or therapeutic approaches for maintenance of a healthy immunological equilibrium.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="45a5585d8c000033a8d66c1a8d8cfbf1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":55277057,"asset_id":35416278,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/55277057/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="35416278"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="35416278"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 35416278; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=35416278]").text(description); $(".js-view-count[data-work-id=35416278]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 35416278; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='35416278']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 35416278, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "45a5585d8c000033a8d66c1a8d8cfbf1" } } $('.js-work-strip[data-work-id=35416278]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":35416278,"title":"PhD Dissertation/Thesis Abstract: Tuning of Human Mucosal Associated Invariant T (MAIT) Cell Function through Microbiota-Mediated T Cell Receptor Signals","translated_title":"","metadata":{"abstract":" Mucosal-associated invariant T (MAIT) cells are a subset of the T cell population defined by an invariant T cell receptor (TCR) that is stimulated by bacterial metabolites. These cells preferentially migrate to mucosal tissues such as gut, liver and skin. MAIT cells are activated by metabolites from the riboflavin (Vitamin B2) biosynthetic pathway that are presented by MHC class 1-related (MR1) molecules expressed on numerous of cell types including antigen presenting cells (APCs) and epithelial cells. The V alpha segment of MAIT TCRs is invariant and composed of Vα7.2, whereas the V beta portion can be variable. MAIT cells are present at very low levels in neonatal blood but significantly increase in adults and show very high variance in their frequency among individuals. While there is evidence that several pathogenic bacteria can activate MAIT cells and that one of their functions is to kill cells with intracellular bacteria, it remains unknown how they discriminate among thousands of commensal bacteria that can also produce Vitamin B2 metabolites. Further, the role of the TCR variable beta region in antigen recognition is still unclear. In this thesis project, we hypothesized that MAIT cells can be stimulated by bacteria that inhabit human mucosal tissues, and that this microbiota-MAIT cell interaction is one of the driving forces in their expansion and variation in the human population. In support of this hypothesis, we observed a reduced proportion of MAIT cells in the blood of perinatally HIV-infected children, which correlated with other microbiota-associated parameters including Th17 cells and inflammatory markers of microbiota alterations. However, it is unclear whether MAIT cells can discriminate bacterial species that reside in the human microbiota, which can produce the riboflavin intermediates. To address this, we developed an in vitro functional assay using human T cells engineered for MAIT-specific TCRs (eMAIT-TCRs) stimulated by MR1-expressing B cell lines presenting the bacterial metabolites. We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded a riboflavin biosynthesis pathway were stimulatory for MAIT-TCRs. Most species that were high-stimulators belonged to Bacteroidetes and Proteobacteria phyla, although with significant variance, whereas low/non-stimulator species were either Actinobacteria or Firmicutes. Furthermore, we determined a wide range of intra-species variation in eMAIT-TCR stimulation capacities of Staphylococcus epidermidis , a skin commensal, which suggests that bacteria can modulate the production capacities of MAIT-TCR stimulatory antigens. Remarkably, we also discovered that human T cells not only express low-levels of MR1 but can also present bacterial metabolites to MAIT cells in an MR1-restricted fashion and trigger TCR signaling to induce cytokine secretion (IFNγ and TNFα), albeit at lower levels. In conclusion, our findings revealed that MAIT cells could discriminate between MR1-restricted, bacteria-derived metabolites through their TCR signaling thresholds. This knowledge paves the way to elucidate the complexity of MAIT cell recognition and its response to the human microbiota, which establishes a framework to explore mechanistic or therapeutic approaches for maintenance of a healthy immunological equilibrium. "},"translated_abstract":" Mucosal-associated invariant T (MAIT) cells are a subset of the T cell population defined by an invariant T cell receptor (TCR) that is stimulated by bacterial metabolites. These cells preferentially migrate to mucosal tissues such as gut, liver and skin. MAIT cells are activated by metabolites from the riboflavin (Vitamin B2) biosynthetic pathway that are presented by MHC class 1-related (MR1) molecules expressed on numerous of cell types including antigen presenting cells (APCs) and epithelial cells. The V alpha segment of MAIT TCRs is invariant and composed of Vα7.2, whereas the V beta portion can be variable. MAIT cells are present at very low levels in neonatal blood but significantly increase in adults and show very high variance in their frequency among individuals. While there is evidence that several pathogenic bacteria can activate MAIT cells and that one of their functions is to kill cells with intracellular bacteria, it remains unknown how they discriminate among thousands of commensal bacteria that can also produce Vitamin B2 metabolites. Further, the role of the TCR variable beta region in antigen recognition is still unclear. In this thesis project, we hypothesized that MAIT cells can be stimulated by bacteria that inhabit human mucosal tissues, and that this microbiota-MAIT cell interaction is one of the driving forces in their expansion and variation in the human population. In support of this hypothesis, we observed a reduced proportion of MAIT cells in the blood of perinatally HIV-infected children, which correlated with other microbiota-associated parameters including Th17 cells and inflammatory markers of microbiota alterations. However, it is unclear whether MAIT cells can discriminate bacterial species that reside in the human microbiota, which can produce the riboflavin intermediates. To address this, we developed an in vitro functional assay using human T cells engineered for MAIT-specific TCRs (eMAIT-TCRs) stimulated by MR1-expressing B cell lines presenting the bacterial metabolites. We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded a riboflavin biosynthesis pathway were stimulatory for MAIT-TCRs. Most species that were high-stimulators belonged to Bacteroidetes and Proteobacteria phyla, although with significant variance, whereas low/non-stimulator species were either Actinobacteria or Firmicutes. Furthermore, we determined a wide range of intra-species variation in eMAIT-TCR stimulation capacities of Staphylococcus epidermidis , a skin commensal, which suggests that bacteria can modulate the production capacities of MAIT-TCR stimulatory antigens. Remarkably, we also discovered that human T cells not only express low-levels of MR1 but can also present bacterial metabolites to MAIT cells in an MR1-restricted fashion and trigger TCR signaling to induce cytokine secretion (IFNγ and TNFα), albeit at lower levels. In conclusion, our findings revealed that MAIT cells could discriminate between MR1-restricted, bacteria-derived metabolites through their TCR signaling thresholds. This knowledge paves the way to elucidate the complexity of MAIT cell recognition and its response to the human microbiota, which establishes a framework to explore mechanistic or therapeutic approaches for maintenance of a healthy immunological equilibrium. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="28098699"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/28098699/HIV_Infected_Children_Have_Lower_Frequencies_of_CD8_Mucosal_Associated_Invariant_T_MAIT_Cells_that_Correlate_with_Innate_Th17_and_Th22_Cell_Subsets"><img alt="Research paper thumbnail of HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets" class="work-thumbnail" src="https://attachments.academia-assets.com/48413308/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/28098699/HIV_Infected_Children_Have_Lower_Frequencies_of_CD8_Mucosal_Associated_Invariant_T_MAIT_Cells_that_Correlate_with_Innate_Th17_and_Th22_Cell_Subsets">HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets</a></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocom-patib...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocom-patibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and-positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity , MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. In this study we evaluated the frequency of MAIT cells in perinatally HIV-infected children, their response to antiretrovi-ral treatment and their associations with HIV clinical status and related innate and adaptive immune cell subsets with potent antibacterial effector functions. We found HIV+ children between ages 3 to 18 years have significantly decreased CD8+ MAIT cell frequencies compared to uninfected healthy children. Remarkably, CD8 MAIT levels gradually increased with antiretroviral therapy, with greater recovery when treatment is initiated at a young age. Moreover, diminished CD8+ MAIT cell frequencies are associated with low CD4:CD8 ratios and elevated sCD14, suggesting a link with HIV disease progression. Last, CD8+ MAIT cell levels tightly correlate with other antibacterial and mucosa-protective immune subsets, namely, neutrophils, innate-like T cells, and Th17 and Th22 cells. Together these findings suggest that low frequencies of MAIT cells in HIV positive children are part of a concerted disruption to the innate and adaptive immune compartments specialized in sensing and responding to pathogenic or commensal bacteria.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3a8772228cc228525a6688d34d4a3b26" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":48413308,"asset_id":28098699,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/48413308/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="28098699"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="28098699"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 28098699; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=28098699]").text(description); $(".js-view-count[data-work-id=28098699]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 28098699; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='28098699']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 28098699, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3a8772228cc228525a6688d34d4a3b26" } } $('.js-work-strip[data-work-id=28098699]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":28098699,"title":"HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets","translated_title":"","metadata":{"doi":"10.1371/journal.pone.0161786","issue":"8","volume":"11","abstract":"Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocom-patibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and-positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity , MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. In this study we evaluated the frequency of MAIT cells in perinatally HIV-infected children, their response to antiretrovi-ral treatment and their associations with HIV clinical status and related innate and adaptive immune cell subsets with potent antibacterial effector functions. We found HIV+ children between ages 3 to 18 years have significantly decreased CD8+ MAIT cell frequencies compared to uninfected healthy children. Remarkably, CD8 MAIT levels gradually increased with antiretroviral therapy, with greater recovery when treatment is initiated at a young age. Moreover, diminished CD8+ MAIT cell frequencies are associated with low CD4:CD8 ratios and elevated sCD14, suggesting a link with HIV disease progression. Last, CD8+ MAIT cell levels tightly correlate with other antibacterial and mucosa-protective immune subsets, namely, neutrophils, innate-like T cells, and Th17 and Th22 cells. Together these findings suggest that low frequencies of MAIT cells in HIV positive children are part of a concerted disruption to the innate and adaptive immune compartments specialized in sensing and responding to pathogenic or commensal bacteria.","page_numbers":"e0161786","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"PLoS ONE"},"translated_abstract":"Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocom-patibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and-positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity , MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. In this study we evaluated the frequency of MAIT cells in perinatally HIV-infected children, their response to antiretrovi-ral treatment and their associations with HIV clinical status and related innate and adaptive immune cell subsets with potent antibacterial effector functions. We found HIV+ children between ages 3 to 18 years have significantly decreased CD8+ MAIT cell frequencies compared to uninfected healthy children. Remarkably, CD8 MAIT levels gradually increased with antiretroviral therapy, with greater recovery when treatment is initiated at a young age. Moreover, diminished CD8+ MAIT cell frequencies are associated with low CD4:CD8 ratios and elevated sCD14, suggesting a link with HIV disease progression. Last, CD8+ MAIT cell levels tightly correlate with other antibacterial and mucosa-protective immune subsets, namely, neutrophils, innate-like T cells, and Th17 and Th22 cells. Together these findings suggest that low frequencies of MAIT cells in HIV positive children are part of a concerted disruption to the innate and adaptive immune compartments specialized in sensing and responding to pathogenic or commensal bacteria.","internal_url":"https://www.academia.edu/28098699/HIV_Infected_Children_Have_Lower_Frequencies_of_CD8_Mucosal_Associated_Invariant_T_MAIT_Cells_that_Correlate_with_Innate_Th17_and_Th22_Cell_Subsets","translated_internal_url":"","created_at":"2016-08-29T09:43:16.889-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[{"id":23838202,"work_id":28098699,"tagging_user_id":20976874,"tagged_user_id":33263908,"co_author_invite_id":null,"email":"a***n@nyumc.org","display_order":1,"name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="37169570"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/37169570/Modifying_astronauts_microbiome_may_enhance_prevention_from_commensal_bacteria_caused_infections_at_microgravity_condition"><img alt="Research paper thumbnail of Modifying astronauts' microbiome may enhance prevention from commensal bacteria caused infections at microgravity condition" class="work-thumbnail" src="https://attachments.academia-assets.com/57120723/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37169570/Modifying_astronauts_microbiome_may_enhance_prevention_from_commensal_bacteria_caused_infections_at_microgravity_condition">Modifying astronauts' microbiome may enhance prevention from commensal bacteria caused infections at microgravity condition</a></div><div class="wp-workCard_item"><span>The Journal of Brief Ideas</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Since in the 2020s, astronauts will be expected to build a colony at Mars without return. And in ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Since in the 2020s, astronauts will be expected to build a colony at Mars without return. And in a report named 'Microbial responses to<br />microgravity and other low-shear environments' by Nickerson et al. at 2004, it is stated that Salmonella typhimurium, known for causing<br />food-borne illness, can change its genome to become more virulent after just a few days in space. That's why modification of astronauts'<br />microbiome gets importance so that infectious diseases might be prevented and antibiotic tolerance might be reduced.<br />On the other hand, a recent report, named 'Salmonella typhimurium intercepts Escherichia coli signaling to enhance antibiotic tolerance',<br />showed that the intestinal pathogen Salmonella typhimurium increases its antibiotic tolerance in response to the bacterial signaling molecule<br />indole, even though Salmonella typhimurium does not natively produce indole. So that this intestinal pathogen can benefit from indole<br />signaling produced by Escherichia coli and/or other intestinal commensal bacteria.<br /><br />To conclude, modifying the indole pathway of Escherichia coli and/or other commensal bacteria of astronauts with the help of synthetic biology techniques may prevent intestinal bacteria caused infections caused by microgravity.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7cfeec238ea3bb7cc084fc42fed9af40" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":57120723,"asset_id":37169570,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/57120723/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37169570"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37169570"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37169570; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37169570]").text(description); $(".js-view-count[data-work-id=37169570]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37169570; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37169570']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37169570, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7cfeec238ea3bb7cc084fc42fed9af40" } } $('.js-work-strip[data-work-id=37169570]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37169570,"title":"Modifying astronauts' microbiome may enhance prevention from commensal bacteria caused infections at microgravity condition","translated_title":"","metadata":{"doi":"10.5281/zenodo.15686","abstract":"Since in the 2020s, astronauts will be expected to build a colony at Mars without return. 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href="https://ku.academia.edu/HassanMatar">Hassan Matar</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">BioGuide is the first designed software that organizes over 1000 parts in parts.igem.org as possi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">BioGuide is the first designed software that organizes over 1000 parts in parts.igem.org as possible atomics parts to build new biological device and systems for specific input and outputs based on graph theory.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a29f0bc0ca4e693efabf8bba292919a8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" 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Sadece 4-5 aylık bir salg...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">COVID-19 salgını, bence dünyayı hızla milenyum çağına zorlamış durumda. Sadece 4-5 aylık bir salgın olmasına karşın en az 670 bilimsel yayın yayımlanmış ve 120’den fazla klinik deneme başvurusu <br />yapılmış durumda. Bu şimdiye kadar gördüğüm en hızlı bilimsel savaş. Ve kısa zamanda COVID-19’un üstesinden  geleceğimizin  habercisi.  Bilim  dünyasında  gerçekleşen  bu  muazzam  hız,  normal hayatımızda kullandığımız teknolojileri de bir anda çağ atlattı. Artık siparişlerimiz drone’larveya otonom araçlarla ile daha sık getirilmeye dünyada başladı bile. 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Projenin ayrıntılarını Ovalı’ya sorduk. Geçen hafta okuduğumuz ‘Küba’nın kanser aşısı artık Türkiye’de’ haberini, aşının ülkemize gelmesine öncülük eden isimlerden, Anadolu Sağlık Merkezi Medikal Onkoloji Uzmanı Prof. Dr. Necdet Üskent’le konuştuk. 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Projenin ayrıntılarını Ovalı’ya sorduk. Geçen hafta okuduğumuz ‘Küba’nın kanser aşısı artık Türkiye’de’ haberini, aşının ülkemize gelmesine öncülük eden isimlerden, Anadolu Sağlık Merkezi Medikal Onkoloji Uzmanı Prof. Dr. Necdet Üskent’le konuştuk. Dünyadaki kanser çalışmalarıyla ilgili gelişmeleri New York Üniversitesi’nde Mikrobiyoloji ve İmmünoloji alanında doktora yapan Cihan Taştan anlattı.","publication_name":"Hürriyet Gazetesi"},"translated_abstract":"Ülkenin sağlık cephesinden sevindirici haberler var: Acıbadem Hücre Laboratuvarı’nın yöneticisi Prof. Dr. Ercüment Ovalı, kanserle mücadelede en başarılı yöntemlerden biri kabul edilen ‘immünoterapi’nin bir çeşidi olan ‘car-t hücre tedavisi’ni yakında Türkiye’de uygulamaya başlayacaklarını duyurdu. Projenin ayrıntılarını Ovalı’ya sorduk. 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Dünyadaki kanser çalışmalarıyla ilgili gelişmeleri New York Üniversitesi’nde Mikrobiyoloji ve İmmünoloji alanında doktora yapan Cihan Taştan anlattı.","internal_url":"https://www.academia.edu/33894920/R%C3%B6portaj_Kanserle_m%C3%BCcadelede_umut_veren_geli%C5%9Fmeler_","translated_internal_url":"","created_at":"2017-07-15T19:37:03.486-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":53927866,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/53927866/thumbnails/1.jpg","file_name":"Kanserle_mucadelede_umut_veren_gelismeler..pdf","download_url":"https://www.academia.edu/attachments/53927866/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Roportaj_Kanserle_mucadelede_umut_veren.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/53927866/Kanserle_mucadelede_umut_veren_gelismeler.-libre.pdf?1500584574=\u0026response-content-disposition=attachment%3B+filename%3DRoportaj_Kanserle_mucadelede_umut_veren.pdf\u0026Expires=1732739027\u0026Signature=eYefJcb0rhIOKQMOrpDNf07eH4s-TGg-nHfPMdtHTyKXeOiSTQWm0oHOGl3yVi~Y1NoBDIVx5cl-l5fnVbTDcTFB9WrH9LSVN~5wdvQ0jgZSepslU0kTN~rVj7ZV6Qxo2fZOQGsa8vZ~UUJCqmopmatIxk16au6APm4BOToWDMd4sEA7c1AD9MpgJPKCP-cXRo7wSICOuC7OFX52LC4GwyUJu0sGNyXhwN6h6UJoUz6k5eaK7cB8pf-diLcO0GpDo05T8DGmj7tVy4xPEiqa-v4MMB7n3mYsMyn78R92hSGESkD2decOdZobC7PFJbnJG5vaCxaYvunPtln6vVm8JA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Röportaj_Kanserle_mücadelede_umut_veren_gelişmeler_","translated_slug":"","page_count":4,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":53927866,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/53927866/thumbnails/1.jpg","file_name":"Kanserle_mucadelede_umut_veren_gelismeler..pdf","download_url":"https://www.academia.edu/attachments/53927866/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Roportaj_Kanserle_mucadelede_umut_veren.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/53927866/Kanserle_mucadelede_umut_veren_gelismeler.-libre.pdf?1500584574=\u0026response-content-disposition=attachment%3B+filename%3DRoportaj_Kanserle_mucadelede_umut_veren.pdf\u0026Expires=1732739027\u0026Signature=eYefJcb0rhIOKQMOrpDNf07eH4s-TGg-nHfPMdtHTyKXeOiSTQWm0oHOGl3yVi~Y1NoBDIVx5cl-l5fnVbTDcTFB9WrH9LSVN~5wdvQ0jgZSepslU0kTN~rVj7ZV6Qxo2fZOQGsa8vZ~UUJCqmopmatIxk16au6APm4BOToWDMd4sEA7c1AD9MpgJPKCP-cXRo7wSICOuC7OFX52LC4GwyUJu0sGNyXhwN6h6UJoUz6k5eaK7cB8pf-diLcO0GpDo05T8DGmj7tVy4xPEiqa-v4MMB7n3mYsMyn78R92hSGESkD2decOdZobC7PFJbnJG5vaCxaYvunPtln6vVm8JA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":775604,"name":"Kanser Tedavisi","url":"https://www.academia.edu/Documents/in/Kanser_Tedavisi"},{"id":895498,"name":"SAĞLIK ALTERNATİF KANSER İMMÜN SİSTEM KUVVETLENDİRİCİ","url":"https://www.academia.edu/Documents/in/SAGLIK_ALTERNATIF_KANSER_IMMUN_SISTEM_KUVVETLENDIRICI"}],"urls":[{"id":8220629,"url":"http://www.hurriyet.com.tr/kanserle-mucadelede-umut-veren-gelismeler-40520785"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="41818440"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/41818440/11_B%C3%B6l%C3%BCm_Yapay_Zeka_ve_Genetik_M%C3%BChendisli%C4%9Fi"><img alt="Research paper thumbnail of 11.Bölüm: Yapay Zeka ve Genetik Mühendisliği" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/41818440/11_B%C3%B6l%C3%BCm_Yapay_Zeka_ve_Genetik_M%C3%BChendisli%C4%9Fi">11.Bölüm: Yapay Zeka ve Genetik Mühendisliği</a></div><div class="wp-workCard_item"><span>Sağlık Bilimlerinde Yapay Zeka </span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Kendi kendini süren arabalardan kanser taramasına, Go oynamaya; aynı zamanda Çin’in Orwellian’ınd...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Kendi kendini süren arabalardan kanser taramasına, Go oynamaya; aynı zamanda Çin’in Orwellian’ından ve algoritma güdümlü izleme programından, ırksal profillemeye, kendi şifrelemesini oluşturan bilgisayarlara, yeni teknolojiler hakkında her gün bir yenisi çıkan başlıklar, bize dünyanın hızla ve tahmin edilemez şekilde değiştiğini göstermektedir. Frost & Sullivan danışman firması, 2021 yılına kadar Yapay Zekâ sistemlerinin dünya genelinde sağlık hizmetlerinden 6,7 milyar dolar gelir elde etmesini öngörmektedir. Makine öğreniminin önemli ölçüde geliştiği alanlardan biri de genomik analizdir: örneğin, bir organizma içindeki tüm gen kümesinin incelenmesi. İnsan sağlığı üzerindeki etkilere çok dikkat edilmesine rağmen, genetik dizilim ve analiz teknolojilerinin gelişmesi, ucuzlaması ve kolaylaşması, tarım ve hayvancılık için de çığır açıcı bir nokta olmuştur. Araştırmacılar, yapay zekâ sistemleri ile daha hızlı, daha ucuz ve daha doğru halde DNA’yı çözümleyebilmekte ve analiz edebilmektedir. Böylelikle, o organizmanın tüm faaliyetlerini düzenleyen belirli genetik plan üzerinde de bir perspektif çizebilmektedirler. Bu kavrayışla hayvanların ıslahı konusunda, bir canlının gelecekte neye toleranslı olabileceği, hangi mutasyonların farklı hastalıklara neden olabileceği ve geleceğe nasıl hazırlanacağı konusunda yapay zekâ ve makine öğrenmesi yüksek isabetli kararlar vermemizi sağlayabilir. Yapay zekâ ve makine öğrenimi uygulamalarındaki gelişmeler sayesinde, araştırmacılar genom dizilimi ve gen düzenleme yoluyla genomik verileri daha iyi yorumlayabilecek hale gelmiştir.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="41818440"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="41818440"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 41818440; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=41818440]").text(description); $(".js-view-count[data-work-id=41818440]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 41818440; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='41818440']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 41818440, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=41818440]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":41818440,"title":"11.Bölüm: Yapay Zeka ve Genetik Mühendisliği","translated_title":"","metadata":{"abstract":"Kendi kendini süren arabalardan kanser taramasına, Go oynamaya; aynı zamanda Çin’in Orwellian’ından ve algoritma güdümlü izleme programından, ırksal profillemeye, kendi şifrelemesini oluşturan bilgisayarlara, yeni teknolojiler hakkında her gün bir yenisi çıkan başlıklar, bize dünyanın hızla ve tahmin edilemez şekilde değiştiğini göstermektedir. 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Bu kavrayışla hayvanların ıslahı konusunda, bir canlının gelecekte neye toleranslı olabileceği, hangi mutasyonların farklı hastalıklara neden olabileceği ve geleceğe nasıl hazırlanacağı konusunda yapay zekâ ve makine öğrenmesi yüksek isabetli kararlar vermemizi sağlayabilir. Yapay zekâ ve makine öğrenimi uygulamalarındaki gelişmeler sayesinde, araştırmacılar genom dizilimi ve gen düzenleme yoluyla genomik verileri daha iyi yorumlayabilecek hale gelmiştir.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"Sağlık Bilimlerinde Yapay Zeka "},"translated_abstract":"Kendi kendini süren arabalardan kanser taramasına, Go oynamaya; aynı zamanda Çin’in Orwellian’ından ve algoritma güdümlü izleme programından, ırksal profillemeye, kendi şifrelemesini oluşturan bilgisayarlara, yeni teknolojiler hakkında her gün bir yenisi çıkan başlıklar, bize dünyanın hızla ve tahmin edilemez şekilde değiştiğini göstermektedir. 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Bu kavrayışla hayvanların ıslahı konusunda, bir canlının gelecekte neye toleranslı olabileceği, hangi mutasyonların farklı hastalıklara neden olabileceği ve geleceğe nasıl hazırlanacağı konusunda yapay zekâ ve makine öğrenmesi yüksek isabetli kararlar vermemizi sağlayabilir. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="35721234"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/35721234/CRISPR_Genom_Modifikasyonlar%C4%B1_T101"><img alt="Research paper thumbnail of CRISPR Genom Modifikasyonları T101" class="work-thumbnail" src="https://attachments.academia-assets.com/55594949/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/35721234/CRISPR_Genom_Modifikasyonlar%C4%B1_T101">CRISPR Genom Modifikasyonları T101</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">CRISPR alanında çalışmalarımız ve popüler bilim yazılarımız arttıkça, sık sık bilim insanları...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">CRISPR alanında çalışmalarımız ve popüler bilim yazılarımız arttıkça, sık sık bilim insanlarından Türkçe kaynak sıkıntısı çektiklerine dair mailler almaya başladım. Bu sebeple, biliminsanlarımıza hem bilimsel bir temel kazandırmak; hem de CRISPR tekniğini gerçekleştirmek üzere gerekli araçlara (plazmid DNA vs.) kolayca ulaşabilmelerini sağlamak için, Uluslararası Plazmid Sağlayacısı Addgene’nin misafir yazarlarla derlemiş olduğu CRISPR 101 kitabının tercüme edilmesine karar verdim. Bu noktada ülkemizdeki yirmiye yakın farklı üniversiteden bilime aç ve aşık öğrenci arkadaşlarımın büyük özveri ve çalışmasıyla, 3 aylık kısa bir vakitte, tercüme kitabımızı tamamlamayı başardık.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e91466ca66de807639d5bc60e1c3bfd4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":55594949,"asset_id":35721234,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/55594949/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="35721234"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="35721234"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 35721234; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=35721234]").text(description); $(".js-view-count[data-work-id=35721234]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 35721234; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='35721234']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 35721234, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e91466ca66de807639d5bc60e1c3bfd4" } } $('.js-work-strip[data-work-id=35721234]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":35721234,"title":"CRISPR Genom Modifikasyonları T101","translated_title":"","metadata":{"abstract":"CRISPR alanında çalışmalarımız ve popüler bilim yazılarımız arttıkça, sık sık bilim insanlarından Türkçe kaynak sıkıntısı çektiklerine dair mailler almaya başladım. Bu sebeple, biliminsanlarımıza hem bilimsel bir temel kazandırmak; hem de CRISPR tekniğini gerçekleştirmek üzere gerekli araçlara (plazmid DNA vs.) kolayca ulaşabilmelerini sağlamak için, Uluslararası Plazmid Sağlayacısı Addgene’nin misafir yazarlarla derlemiş olduğu CRISPR 101 kitabının tercüme edilmesine karar verdim. Bu noktada ülkemizdeki yirmiye yakın farklı üniversiteden bilime aç ve aşık öğrenci arkadaşlarımın büyük özveri ve çalışmasıyla, 3 aylık kısa bir vakitte, tercüme kitabımızı tamamlamayı başardık."},"translated_abstract":"CRISPR alanında çalışmalarımız ve popüler bilim yazılarımız arttıkça, sık sık bilim insanlarından Türkçe kaynak sıkıntısı çektiklerine dair mailler almaya başladım. Bu sebeple, biliminsanlarımıza hem bilimsel bir temel kazandırmak; hem de CRISPR tekniğini gerçekleştirmek üzere gerekli araçlara (plazmid DNA vs.) kolayca ulaşabilmelerini sağlamak için, Uluslararası Plazmid Sağlayacısı Addgene’nin misafir yazarlarla derlemiş olduğu CRISPR 101 kitabının tercüme edilmesine karar verdim. Bu noktada ülkemizdeki yirmiye yakın farklı üniversiteden bilime aç ve aşık öğrenci arkadaşlarımın büyük özveri ve çalışmasıyla, 3 aylık kısa bir vakitte, tercüme kitabımızı tamamlamayı başardık.","internal_url":"https://www.academia.edu/35721234/CRISPR_Genom_Modifikasyonlar%C4%B1_T101","translated_internal_url":"","created_at":"2018-01-21T06:01:09.435-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[],"downloadable_attachments":[{"id":55594949,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/55594949/thumbnails/1.jpg","file_name":"CRISPR_Gen_Modifikasyonlari_T101.final.pdf","download_url":"https://www.academia.edu/attachments/55594949/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CRISPR_Genom_Modifikasyonlari_T101.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/55594949/CRISPR_Gen_Modifikasyonlari_T101.final-libre.pdf?1516543828=\u0026response-content-disposition=attachment%3B+filename%3DCRISPR_Genom_Modifikasyonlari_T101.pdf\u0026Expires=1732739028\u0026Signature=gEORiRvnnb9Q5vo23QTlbwWVCFDlo2SljnD~OZErz3nv4qH7LbVm8AKH6ucI3oatGGuEK-Y9lwfu~2jKqe~z5jRSmyPQVgjWSiIXmdQlKcv6jt65XYvlAZBrQ71CVzE3wfH49SADIZXfi8nxbUxWtFSL9vamKNYWf~gMMmlOwoSxJ1kvDXkVqS546NaZw5ENhWpUYPLTQKz2NYOWh7sGAv1lJjzTd0bquotPVOGQqWWuXria5Ko0efICONJCkoqV4GOalMCl87Nhw7s-N9lKbswK6iV3PRZHytM9zsE5ycY2KIsparQ13XpvC27j3JC-0v0Z7Y7SA90dTdqiSnDuxA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"CRISPR_Genom_Modifikasyonları_T101","translated_slug":"","page_count":255,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":55594949,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/55594949/thumbnails/1.jpg","file_name":"CRISPR_Gen_Modifikasyonlari_T101.final.pdf","download_url":"https://www.academia.edu/attachments/55594949/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CRISPR_Genom_Modifikasyonlari_T101.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/55594949/CRISPR_Gen_Modifikasyonlari_T101.final-libre.pdf?1516543828=\u0026response-content-disposition=attachment%3B+filename%3DCRISPR_Genom_Modifikasyonlari_T101.pdf\u0026Expires=1732739028\u0026Signature=gEORiRvnnb9Q5vo23QTlbwWVCFDlo2SljnD~OZErz3nv4qH7LbVm8AKH6ucI3oatGGuEK-Y9lwfu~2jKqe~z5jRSmyPQVgjWSiIXmdQlKcv6jt65XYvlAZBrQ71CVzE3wfH49SADIZXfi8nxbUxWtFSL9vamKNYWf~gMMmlOwoSxJ1kvDXkVqS546NaZw5ENhWpUYPLTQKz2NYOWh7sGAv1lJjzTd0bquotPVOGQqWWuXria5Ko0efICONJCkoqV4GOalMCl87Nhw7s-N9lKbswK6iV3PRZHytM9zsE5ycY2KIsparQ13XpvC27j3JC-0v0Z7Y7SA90dTdqiSnDuxA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":70125,"name":"CRISPR","url":"https://www.academia.edu/Documents/in/CRISPR"},{"id":594414,"name":"CRISPR Systems","url":"https://www.academia.edu/Documents/in/CRISPR_Systems"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="4774881" id="popülerbilimmakaleleri"><div class="js-work-strip profile--work_container" data-work-id="41657317"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/41657317/CRISPR_Gen_M%C3%BChendisli%C4%9Fi_ve_Hen%C3%BCz_G%C3%B6remedi%C4%9Fimiz_Karanl%C4%B1k_Y%C3%BCz%C3%BC"><img alt="Research paper thumbnail of CRISPR Gen Mühendisliği ve Henüz Göremediğimiz Karanlık Yüzü" class="work-thumbnail" src="https://attachments.academia-assets.com/61810922/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/41657317/CRISPR_Gen_M%C3%BChendisli%C4%9Fi_ve_Hen%C3%BCz_G%C3%B6remedi%C4%9Fimiz_Karanl%C4%B1k_Y%C3%BCz%C3%BC">CRISPR Gen Mühendisliği ve Henüz Göremediğimiz Karanlık Yüzü</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/CemLeonMenase">Cem Leon Menase</a></span></div><div class="wp-workCard_item"><span>Başlangıç Noktası (Blog)</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Üzerinde daha çok durulması gereken bilinmezliklerin başında gelenlerden biri CRISPR. Biyoteknolo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Üzerinde daha çok durulması gereken bilinmezliklerin başında gelenlerden biri CRISPR. Biyoteknolojinin büyük umut vadeden çıktılarından CRISPR, Mars’a gitmeye benziyor. Doğal kaynaklardan insan ırkının devamlılığına kadar birçok faydası olabilir. Fakat aynı zamanda henüz gitmediğimiz için bir bilinmez. Bu bilinmezliğin getirdiği birçok risk ve muhtemelen tehlike var. Üstelik konu sağlık olunca, bileşenleri ve etkileşimleri daha iyi anlamak ve takip etmek daha da önemli hale geliyor. Yoksa ağlar bize bir “salgın” ile geri dönebiliyor. CRISPR da böyle altını kazımamız gereken bir alan. Bu konuyu, alanın uzmanı Dr. Cihan Taştan’dan dinlemek en doğrusu.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d129fd1f44b51393ff91ac4f35a97186" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61810922,"asset_id":41657317,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61810922/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="41657317"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="41657317"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 41657317; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=41657317]").text(description); $(".js-view-count[data-work-id=41657317]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 41657317; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='41657317']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 41657317, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d129fd1f44b51393ff91ac4f35a97186" } } $('.js-work-strip[data-work-id=41657317]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":41657317,"title":"CRISPR Gen Mühendisliği ve Henüz Göremediğimiz Karanlık Yüzü","translated_title":"","metadata":{"abstract":"Üzerinde daha çok durulması gereken bilinmezliklerin başında gelenlerden biri CRISPR. Biyoteknolojinin büyük umut vadeden çıktılarından CRISPR, Mars’a gitmeye benziyor. Doğal kaynaklardan insan ırkının devamlılığına kadar birçok faydası olabilir. Fakat aynı zamanda henüz gitmediğimiz için bir bilinmez. Bu bilinmezliğin getirdiği birçok risk ve muhtemelen tehlike var. Üstelik konu sağlık olunca, bileşenleri ve etkileşimleri daha iyi anlamak ve takip etmek daha da önemli hale geliyor. Yoksa ağlar bize bir “salgın” ile geri dönebiliyor. CRISPR da böyle altını kazımamız gereken bir alan. Bu konuyu, alanın uzmanı Dr. Cihan Taştan’dan dinlemek en doğrusu.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Başlangıç Noktası (Blog)"},"translated_abstract":"Üzerinde daha çok durulması gereken bilinmezliklerin başında gelenlerden biri CRISPR. Biyoteknolojinin büyük umut vadeden çıktılarından CRISPR, Mars’a gitmeye benziyor. Doğal kaynaklardan insan ırkının devamlılığına kadar birçok faydası olabilir. Fakat aynı zamanda henüz gitmediğimiz için bir bilinmez. Bu bilinmezliğin getirdiği birçok risk ve muhtemelen tehlike var. Üstelik konu sağlık olunca, bileşenleri ve etkileşimleri daha iyi anlamak ve takip etmek daha da önemli hale geliyor. Yoksa ağlar bize bir “salgın” ile geri dönebiliyor. CRISPR da böyle altını kazımamız gereken bir alan. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="41326576"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/41326576/Aral%C4%B1k_2019_Nature_Makale_%C3%87evirisi_Onayl%C4%B1_Genetik_Tedavi_%C4%B0la%C3%A7lar%C4%B1nda_Son_Durum"><img alt="Research paper thumbnail of Aralık 2019 Nature Makale Çevirisi: Onaylı Genetik Tedavi İlaçlarında Son Durum" class="work-thumbnail" src="https://attachments.academia-assets.com/61531209/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/41326576/Aral%C4%B1k_2019_Nature_Makale_%C3%87evirisi_Onayl%C4%B1_Genetik_Tedavi_%C4%B0la%C3%A7lar%C4%B1nda_Son_Durum">Aralık 2019 Nature Makale Çevirisi: Onaylı Genetik Tedavi İlaçlarında Son Durum</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Gen tedavisi fikri - mutasyona uğramış, hastalığa neden olan bir değişkeni değiştirmek için hücre...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Gen tedavisi fikri - mutasyona uğramış, hastalığa neden olan bir değişkeni değiştirmek için hücrelere sağlıklı bir gen ekleyen bir DNA tabanlı ilaç türü - olarak ilk 1972'de yayınlandı. Onlarca yıl süren tartışmalardan, deneysel denemelerdeki tedavi başarısızlıklardan ve bazı ölümlerden sonra, bir tür cilt kanseri için ilk gen terapisi ilacı, 2003 yılında Çin'de onaylandı. Dünyanın geri kalanı, bu faydalardan kolayca ikna olmadı ve ta ki 2017'de, ABD bu genetik tedavi ilaçlarından birini onaylayana dek. O zamandan beri, onaylama hızı çok hızlı bir şekilde arttı. Bazı kanser türleri, bazı virüs enfeksiyonları ve kalıtsal rahatsızlıklar için en az dokuz gen terapisi onaylandı. İlgili bir ilaç tipi, çalışmalarını engellemek için DNA veya RNA uzantıları kullanarak hatalı genlere müdahale eder. Neredeyse yarım yüzyıl sonra, genetik tıp kavramı bir gerçek haline geldi.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1dc72def6ae95175cc15e5f91461159b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61531209,"asset_id":41326576,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61531209/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="41326576"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="41326576"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 41326576; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=41326576]").text(description); $(".js-view-count[data-work-id=41326576]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 41326576; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='41326576']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 41326576, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1dc72def6ae95175cc15e5f91461159b" } } $('.js-work-strip[data-work-id=41326576]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":41326576,"title":"Aralık 2019 Nature Makale Çevirisi: Onaylı Genetik Tedavi İlaçlarında Son Durum","translated_title":"","metadata":{"abstract":"Gen tedavisi fikri - mutasyona uğramış, hastalığa neden olan bir değişkeni değiştirmek için hücrelere sağlıklı bir gen ekleyen bir DNA tabanlı ilaç türü - olarak ilk 1972'de yayınlandı. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="41231767"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/41231767/Yeni_Nesil_CRISPR_D%C3%BCzenleme_Arac%C4%B1_Prime_Editing_"><img alt="Research paper thumbnail of Yeni Nesil CRISPR Düzenleme Aracı “Prime Editing”" class="work-thumbnail" src="https://attachments.academia-assets.com/61462058/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/41231767/Yeni_Nesil_CRISPR_D%C3%BCzenleme_Arac%C4%B1_Prime_Editing_">Yeni Nesil CRISPR Düzenleme Aracı “Prime Editing”</a></div><div class="wp-workCard_item"><span>İDEAPORT BLOG</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">2012 yılı itibariyle geliştirilmeye başlanan CRISPR-Cas gen düzenleme sisteminin, geçen yedi yıld...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">2012 yılı itibariyle geliştirilmeye başlanan CRISPR-Cas gen düzenleme sisteminin, geçen yedi yılda birçok yeni nesil versiyonları üretildi. Ancak şimdiye kadar bu sistemin hiçbir versiyonu, "Prime Editing" (PM) adı verilen yeni gen düzenleme teknolojisi kadar heyecan vermedi.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="04d095615b1ca4fc2fcf924952cc13f7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61462058,"asset_id":41231767,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61462058/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="41231767"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="41231767"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 41231767; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=41231767]").text(description); $(".js-view-count[data-work-id=41231767]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 41231767; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='41231767']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 41231767, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "04d095615b1ca4fc2fcf924952cc13f7" } } $('.js-work-strip[data-work-id=41231767]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":41231767,"title":"Yeni Nesil CRISPR Düzenleme Aracı “Prime Editing”","translated_title":"","metadata":{"abstract":"2012 yılı itibariyle geliştirilmeye başlanan CRISPR-Cas gen düzenleme sisteminin, geçen yedi yılda birçok yeni nesil versiyonları üretildi. Ancak şimdiye kadar bu sistemin hiçbir versiyonu, \"Prime Editing\" (PM) adı verilen yeni gen düzenleme teknolojisi kadar heyecan vermedi. ","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"İDEAPORT BLOG"},"translated_abstract":"2012 yılı itibariyle geliştirilmeye başlanan CRISPR-Cas gen düzenleme sisteminin, geçen yedi yılda birçok yeni nesil versiyonları üretildi. Ancak şimdiye kadar bu sistemin hiçbir versiyonu, \"Prime Editing\" (PM) adı verilen yeni gen düzenleme teknolojisi kadar heyecan vermedi. 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HiDNA'nın Doğuşu: Kalem Ucu Kadar DNA'da Anılar, Banka Hesapları ve Saklamak istediğiniz Her Şeyi depolayın!" class="work-thumbnail" src="https://attachments.academia-assets.com/59257994/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/39129541/1_0_3_HiDNAn%C4%B1n_Do%C4%9Fu%C5%9Fu_Kalem_Ucu_Kadar_DNAda_An%C4%B1lar_Banka_Hesaplar%C4%B1_ve_Saklamak_istedi%C4%9Finiz_Her_%C5%9Eeyi_depolay%C4%B1n_">1.0.3. HiDNA'nın Doğuşu: Kalem Ucu Kadar DNA'da Anılar, Banka Hesapları ve Saklamak istediğiniz Her Şeyi depolayın!</a></div><div class="wp-workCard_item"><span>Bezelye Dergi Blog</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Günümüzde dijital veri üretimi (ve veri depolama), örneğin video içerikleri, nesnelerin interneti...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Günümüzde dijital veri üretimi (ve veri depolama), örneğin video içerikleri, nesnelerin interneti ve <br />sosyal medya gibi, olağan üstü bir hızla artıyor. 2017’de dünya nüfusunun %51’i internete <br />ulaşabiliyorken; tahminler, dünya nüfusunun tamamına yakınının 2030 yılına kadar on-line <br />olacağına işaret ediyor. 2018'de, küresel veri depolaması, 2010'da 1 zettabayttan 33 zettabayta <br />ulaştı. Tahminler, bunun 2040 yılına kadar 6-19 yottabyte’a ulaşabileceğini gösteriyor. Bu gidişle <br />mevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi <br />bekleniyor. Aynı zamanda, yarı iletken üretimi zaten Moore Yasasının gösterdiği limitlere ulaşmış <br />durumda. Dijital veri depolamada ulaşabileceğimiz fiziksel sınırlar, enerji tüketimi ve potansiyel <br />hammadde zorlukları, daha fazla depolama için tarihsel olarak azalan fiyatlara rağmen, <br />önümüzdeki 10-20 yıl içinde daha farklı veri depolama yöntemlerine ihtiyacımız olduğunu <br />gösteriyor. Dünya, mevcut teknolojiler ile üretilen verilerin etkin depolanmasını logaritmik <br />iyileştirmelerle sağlayabilir; yine de daha geniş depolama ortamlarının hızla geliştirilmesi yalın bir <br />gerçek olarak önümüzde duruyor. DNA, işte bu dünya kadar verinin hepsini depolamanın son <br />derece kompakt bir yolu olarak ortaya çıktı.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e5fddf5cc73bcc0df510f0e1ccab02ac" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":59257994,"asset_id":39129541,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/59257994/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="39129541"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="39129541"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 39129541; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=39129541]").text(description); $(".js-view-count[data-work-id=39129541]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 39129541; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='39129541']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 39129541, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e5fddf5cc73bcc0df510f0e1ccab02ac" } } $('.js-work-strip[data-work-id=39129541]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":39129541,"title":"1.0.3. HiDNA'nın Doğuşu: Kalem Ucu Kadar DNA'da Anılar, Banka Hesapları ve Saklamak istediğiniz Her Şeyi depolayın!","translated_title":"","metadata":{"abstract":"Günümüzde dijital veri üretimi (ve veri depolama), örneğin video içerikleri, nesnelerin interneti ve \r\nsosyal medya gibi, olağan üstü bir hızla artıyor. 2017’de dünya nüfusunun %51’i internete \r\nulaşabiliyorken; tahminler, dünya nüfusunun tamamına yakınının 2030 yılına kadar on-line \r\nolacağına işaret ediyor. 2018'de, küresel veri depolaması, 2010'da 1 zettabayttan 33 zettabayta \r\nulaştı. Tahminler, bunun 2040 yılına kadar 6-19 yottabyte’a ulaşabileceğini gösteriyor. Bu gidişle\r\nmevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi \r\nbekleniyor. Aynı zamanda, yarı iletken üretimi zaten Moore Yasasının gösterdiği limitlere ulaşmış \r\ndurumda. Dijital veri depolamada ulaşabileceğimiz fiziksel sınırlar, enerji tüketimi ve potansiyel \r\nhammadde zorlukları, daha fazla depolama için tarihsel olarak azalan fiyatlara rağmen,\r\nönümüzdeki 10-20 yıl içinde daha farklı veri depolama yöntemlerine ihtiyacımız olduğunu\r\ngösteriyor. Dünya, mevcut teknolojiler ile üretilen verilerin etkin depolanmasını logaritmik \r\niyileştirmelerle sağlayabilir; yine de daha geniş depolama ortamlarının hızla geliştirilmesi yalın bir \r\ngerçek olarak önümüzde duruyor. DNA, işte bu dünya kadar verinin hepsini depolamanın son \r\nderece kompakt bir yolu olarak ortaya çıktı. ","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"Bezelye Dergi Blog"},"translated_abstract":"Günümüzde dijital veri üretimi (ve veri depolama), örneğin video içerikleri, nesnelerin interneti ve \r\nsosyal medya gibi, olağan üstü bir hızla artıyor. 2017’de dünya nüfusunun %51’i internete \r\nulaşabiliyorken; tahminler, dünya nüfusunun tamamına yakınının 2030 yılına kadar on-line \r\nolacağına işaret ediyor. 2018'de, küresel veri depolaması, 2010'da 1 zettabayttan 33 zettabayta \r\nulaştı. Tahminler, bunun 2040 yılına kadar 6-19 yottabyte’a ulaşabileceğini gösteriyor. Bu gidişle\r\nmevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi \r\nbekleniyor. Aynı zamanda, yarı iletken üretimi zaten Moore Yasasının gösterdiği limitlere ulaşmış \r\ndurumda. Dijital veri depolamada ulaşabileceğimiz fiziksel sınırlar, enerji tüketimi ve potansiyel \r\nhammadde zorlukları, daha fazla depolama için tarihsel olarak azalan fiyatlara rağmen,\r\nönümüzdeki 10-20 yıl içinde daha farklı veri depolama yöntemlerine ihtiyacımız olduğunu\r\ngösteriyor. Dünya, mevcut teknolojiler ile üretilen verilerin etkin depolanmasını logaritmik \r\niyileştirmelerle sağlayabilir; yine de daha geniş depolama ortamlarının hızla geliştirilmesi yalın bir \r\ngerçek olarak önümüzde duruyor. DNA, işte bu dünya kadar verinin hepsini depolamanın son \r\nderece kompakt bir yolu olarak ortaya çıktı. 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HiDNA'nın Doğuşu: DNA'da Veri Depolama Teknolojisinin Dünü, Bugünü ve Yarını" class="work-thumbnail" src="https://attachments.academia-assets.com/59109731/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/39002176/1_0_2_HiDNAn%C4%B1n_Do%C4%9Fu%C5%9Fu_DNAda_Veri_Depolama_Teknolojisinin_D%C3%BCn%C3%BC_Bug%C3%BCn%C3%BC_ve_Yar%C4%B1n%C4%B1">1.0.2. HiDNA'nın Doğuşu: DNA'da Veri Depolama Teknolojisinin Dünü, Bugünü ve Yarını</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Modern dünya, her gün tsunami kadar veriyle karşı karşıya. 2013’te insanlar 4.4 zettabayt veri ür...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Modern dünya, her gün tsunami kadar veriyle karşı karşıya. 2013’te insanlar 4.4 zettabayt veri üretmişti; 2025'te ise 160 zettabayt ile bilgi patlaması olacağı hesaplanıyor. Bu gidişle mevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi bekleniyor. Bu nedenle, mevcut teknolojik altyapının bu veri tufanının sadece bir kısmına yetebileceği öngörülüyor. DNA işte bu dünya kadar verinin hepsini depolamanın son derece kompakt bir yolu olarak ortaya çıktı.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ae632f032f1ac8798513941b180974f4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":59109731,"asset_id":39002176,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/59109731/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="39002176"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="39002176"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 39002176; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=39002176]").text(description); $(".js-view-count[data-work-id=39002176]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 39002176; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='39002176']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 39002176, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ae632f032f1ac8798513941b180974f4" } } $('.js-work-strip[data-work-id=39002176]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":39002176,"title":"1.0.2. HiDNA'nın Doğuşu: DNA'da Veri Depolama Teknolojisinin Dünü, Bugünü ve Yarını","translated_title":"","metadata":{"abstract":"Modern dünya, her gün tsunami kadar veriyle karşı karşıya. 2013’te insanlar 4.4 zettabayt veri üretmişti; 2025'te ise 160 zettabayt ile bilgi patlaması olacağı hesaplanıyor. Bu gidişle mevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi bekleniyor. Bu nedenle, mevcut teknolojik altyapının bu veri tufanının sadece bir kısmına yetebileceği öngörülüyor. DNA işte bu dünya kadar verinin hepsini depolamanın son derece kompakt bir yolu olarak ortaya çıktı."},"translated_abstract":"Modern dünya, her gün tsunami kadar veriyle karşı karşıya. 2013’te insanlar 4.4 zettabayt veri üretmişti; 2025'te ise 160 zettabayt ile bilgi patlaması olacağı hesaplanıyor. Bu gidişle mevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi bekleniyor. Bu nedenle, mevcut teknolojik altyapının bu veri tufanının sadece bir kısmına yetebileceği öngörülüyor. DNA işte bu dünya kadar verinin hepsini depolamanın son derece kompakt bir yolu olarak ortaya çıktı.","internal_url":"https://www.academia.edu/39002176/1_0_2_HiDNAn%C4%B1n_Do%C4%9Fu%C5%9Fu_DNAda_Veri_Depolama_Teknolojisinin_D%C3%BCn%C3%BC_Bug%C3%BCn%C3%BC_ve_Yar%C4%B1n%C4%B1","translated_internal_url":"","created_at":"2019-05-02T07:06:03.435-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[],"downloadable_attachments":[{"id":59109731,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/59109731/thumbnails/1.jpg","file_name":"HiDNA_1.0.2.DNAda_Veri_Depolama_Teknolojisi.30.04.1920190502-90297-1l21yq1.pdf","download_url":"https://www.academia.edu/attachments/59109731/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"1_0_2_HiDNAnin_Dogusu_DNAda_Veri_Depolam.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/59109731/HiDNA_1.0.2.DNAda_Veri_Depolama_Teknolojisi.30.04.1920190502-90297-1l21yq1-libre.pdf?1556807210=\u0026response-content-disposition=attachment%3B+filename%3D1_0_2_HiDNAnin_Dogusu_DNAda_Veri_Depolam.pdf\u0026Expires=1732739028\u0026Signature=Jk6GVHFkNA701QHSxx5PYPnGJuvIcul8gUVXY34q6flcL852hi9c7WbXgZjUZLkqITv8oIBNyp~78COXY8nvNP75Na8uZLWD9Z-Z~REQB4-pZQL9DdnHXc~Eo5R35HirXPGQ2pIPH3FYUYK~X4WgtXwbTtY2eVA8vTulQiUjCZchWfOSbP~ggh3aTwOq3i3flASIbSdBCkXuZMp4deAonX1vhNrAuVLqz7ykjJSkGLahT5Y1n2D9UKaIqEKXFYRAgMsDeE-fW1-I0CwaktyjkTo8hx4Vwk0SSefNGT9~UlXELGdpXX5mSjPzifCsze43bN5IuYpc8~fF61pHe1d-FA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"1_0_2_HiDNAnın_Doğuşu_DNAda_Veri_Depolama_Teknolojisinin_Dünü_Bugünü_ve_Yarını","translated_slug":"","page_count":6,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":59109731,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/59109731/thumbnails/1.jpg","file_name":"HiDNA_1.0.2.DNAda_Veri_Depolama_Teknolojisi.30.04.1920190502-90297-1l21yq1.pdf","download_url":"https://www.academia.edu/attachments/59109731/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"1_0_2_HiDNAnin_Dogusu_DNAda_Veri_Depolam.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/59109731/HiDNA_1.0.2.DNAda_Veri_Depolama_Teknolojisi.30.04.1920190502-90297-1l21yq1-libre.pdf?1556807210=\u0026response-content-disposition=attachment%3B+filename%3D1_0_2_HiDNAnin_Dogusu_DNAda_Veri_Depolam.pdf\u0026Expires=1732739028\u0026Signature=Jk6GVHFkNA701QHSxx5PYPnGJuvIcul8gUVXY34q6flcL852hi9c7WbXgZjUZLkqITv8oIBNyp~78COXY8nvNP75Na8uZLWD9Z-Z~REQB4-pZQL9DdnHXc~Eo5R35HirXPGQ2pIPH3FYUYK~X4WgtXwbTtY2eVA8vTulQiUjCZchWfOSbP~ggh3aTwOq3i3flASIbSdBCkXuZMp4deAonX1vhNrAuVLqz7ykjJSkGLahT5Y1n2D9UKaIqEKXFYRAgMsDeE-fW1-I0CwaktyjkTo8hx4Vwk0SSefNGT9~UlXELGdpXX5mSjPzifCsze43bN5IuYpc8~fF61pHe1d-FA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":41012,"name":"Recombinant DNA Technology","url":"https://www.academia.edu/Documents/in/Recombinant_DNA_Technology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="39002167"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/39002167/1_0_1_HiDNAn%C4%B1n_Do%C4%9Fu%C5%9Fu_DNAda_Veri_Depolama_Teknolojisi"><img alt="Research paper thumbnail of 1.0.1. HiDNA'nın Doğuşu: DNA'da Veri Depolama Teknolojisi" class="work-thumbnail" src="https://attachments.academia-assets.com/59420100/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/39002167/1_0_1_HiDNAn%C4%B1n_Do%C4%9Fu%C5%9Fu_DNAda_Veri_Depolama_Teknolojisi">1.0.1. HiDNA'nın Doğuşu: DNA'da Veri Depolama Teknolojisi</a></div><div class="wp-workCard_item"><span>Gen İzi Dergisi</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Modern dünya, her gün tsunami kadar veriyle karşı karşıya. 2013’te insanlar 4.4 zettabayt veri ür...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Modern dünya, her gün tsunami kadar veriyle karşı karşıya. 2013’te insanlar 4.4 zettabayt veri üretmişti; 2025'te ise 160 zettabayt ile bilgi patlaması olacağı hesaplanıyor. Ancak mevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi bekleniyor. Bu nedenle, mevcut teknolojik altyapının bu veri tufanının sadece bir kısmına yetebileceği öngörülüyor. DNA işte bu dünya kadar verinin hepsini depolamanın son derece kompakt bir yolu olarak ortaya çıktı.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b1a424d798c941884196acfdac44e729" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":59420100,"asset_id":39002167,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/59420100/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="39002167"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="39002167"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 39002167; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=39002167]").text(description); $(".js-view-count[data-work-id=39002167]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 39002167; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='39002167']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 39002167, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b1a424d798c941884196acfdac44e729" } } $('.js-work-strip[data-work-id=39002167]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":39002167,"title":"1.0.1. HiDNA'nın Doğuşu: DNA'da Veri Depolama Teknolojisi","translated_title":"","metadata":{"volume":"1","abstract":"Modern dünya, her gün tsunami kadar veriyle karşı karşıya. 2013’te insanlar 4.4 zettabayt veri üretmişti; 2025'te ise 160 zettabayt ile bilgi patlaması olacağı hesaplanıyor. Ancak mevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi bekleniyor. Bu nedenle, mevcut teknolojik altyapının bu veri tufanının sadece bir kısmına yetebileceği öngörülüyor. DNA işte bu dünya kadar verinin hepsini depolamanın son derece kompakt bir yolu olarak ortaya çıktı.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"Gen İzi Dergisi"},"translated_abstract":"Modern dünya, her gün tsunami kadar veriyle karşı karşıya. 2013’te insanlar 4.4 zettabayt veri üretmişti; 2025'te ise 160 zettabayt ile bilgi patlaması olacağı hesaplanıyor. Ancak mevcut altyapı ile 2040 yılına kadar tüm dünyadaki mikroçip dereceli silikonun tükenmesi bekleniyor. Bu nedenle, mevcut teknolojik altyapının bu veri tufanının sadece bir kısmına yetebileceği öngörülüyor. 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Hastalıklar üzerine ilaç geliştirme çalışmalarını anlattıktan sonra katılımcılardan dikkat çeken bir soru yöneldi: Afrika'da birçok hastalık mevcut ve daha bunlar üzerine hiçbir ilaç araçtırma – geliştirme çalışması yapılmamış. Neden ilaç şirketleri hastalık portföyünü ve ilaç yatırımlarını genişletmiyor? Yöneticinin verdiği cevap etik görünmese de şirketlerin hastalıklardan muzdarip insanlara yaklaşımını özetlediği için trajikti: Birçok hastalık için araştırma yatırımı yapılsa Afrika'daki bu hastalıklara da ilaçlar geliştirilebilir. Ancak satılacak bu ilaçların alıcılarının maddi imkanlarını ve satılabilecek ilaç miktarının küçüklüğünden ötürü birçok hastalık için herhangi bir çalışma gerçekleştirilmiyor. Bu durum özellikle genetik/ailesel/kalıtsal hastalıklar için kaçınılmaz bir gerçekti. Ta ki yine 2013 yılında kolay, ucuz ve yüksek ulaşılabilirlikte CRISPR genom modifikasyon tekniğinin geliştirilmesiyle, insan hücrelerinin genetiğinin tamir edilebileceğini gösteren bilimsel makaleler yayınlanıncaya dek. Bu vakitten sonra ilaç şirketleri AR-GE çalışmalarını genetik tedaviler üzerine yoğunlaştırmaya başladı. Birçok biyoteknoloji şirketi nadir ve halk arasında tedavi edilmesi olanaksız hastalıkların gen terapi metotlarıyla tamir etmek üzerine amansız bir yarışa atıldı. Aslına bakarsanız CRISPR genom modifikasyon mekanizması 1987'de bakteri genomunda keşfedildi ve tekrarlı DNA dizilerinden oluşmalarından ötürü CRISPR olarak adlandırıldı. Bu dizilerin tam olarak fonksiyonunun anlaşılması için yirmi yıl geçmesi gerekti ve 2007'de bakterilerin virüslere karşı sahip olduğu bağışıklık sistemi olduğu anlaşıldı. 2013'te Nobel ödülünün olası en büyük adaylarından Feng Zhang ve Jennifer Doudna, CRISPR sistemini insan genetiğini değiştirmek hatta tamir edebilmek için optimize ettiğini duyurdu. Takip eden beş yıllık süreçte dünya üzerinde bu tekniği kullanarak insan, hayvan, bitki ve böcek olmak üzere birçok canlı türünün genetiğini başarıyla modifiye edebildiğini rapor eden binlerce bilimsel makale yayınlandı.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c74e5ad02821e421f7384e2a1156c7bb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56829839,"asset_id":36845381,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56829839/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36845381"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36845381"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36845381; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36845381]").text(description); $(".js-view-count[data-work-id=36845381]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36845381; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36845381']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36845381, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c74e5ad02821e421f7384e2a1156c7bb" } } $('.js-work-strip[data-work-id=36845381]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36845381,"title":"Tamiri İmkânsız Sanılan Genetik Hastalıklara Gen Terapi Olanağı!","translated_title":"","metadata":{"abstract":"2013 yılının ilk aylarında New York Üniversitesinde katıldığım bir derse ilaç şirketlerinden yöneticiler davet edilmişti. Hastalıklar üzerine ilaç geliştirme çalışmalarını anlattıktan sonra katılımcılardan dikkat çeken bir soru yöneldi: Afrika'da birçok hastalık mevcut ve daha bunlar üzerine hiçbir ilaç araçtırma – geliştirme çalışması yapılmamış. Neden ilaç şirketleri hastalık portföyünü ve ilaç yatırımlarını genişletmiyor? Yöneticinin verdiği cevap etik görünmese de şirketlerin hastalıklardan muzdarip insanlara yaklaşımını özetlediği için trajikti: Birçok hastalık için araştırma yatırımı yapılsa Afrika'daki bu hastalıklara da ilaçlar geliştirilebilir. Ancak satılacak bu ilaçların alıcılarının maddi imkanlarını ve satılabilecek ilaç miktarının küçüklüğünden ötürü birçok hastalık için herhangi bir çalışma gerçekleştirilmiyor. Bu durum özellikle genetik/ailesel/kalıtsal hastalıklar için kaçınılmaz bir gerçekti. Ta ki yine 2013 yılında kolay, ucuz ve yüksek ulaşılabilirlikte CRISPR genom modifikasyon tekniğinin geliştirilmesiyle, insan hücrelerinin genetiğinin tamir edilebileceğini gösteren bilimsel makaleler yayınlanıncaya dek. Bu vakitten sonra ilaç şirketleri AR-GE çalışmalarını genetik tedaviler üzerine yoğunlaştırmaya başladı. Birçok biyoteknoloji şirketi nadir ve halk arasında tedavi edilmesi olanaksız hastalıkların gen terapi metotlarıyla tamir etmek üzerine amansız bir yarışa atıldı. Aslına bakarsanız CRISPR genom modifikasyon mekanizması 1987'de bakteri genomunda keşfedildi ve tekrarlı DNA dizilerinden oluşmalarından ötürü CRISPR olarak adlandırıldı. Bu dizilerin tam olarak fonksiyonunun anlaşılması için yirmi yıl geçmesi gerekti ve 2007'de bakterilerin virüslere karşı sahip olduğu bağışıklık sistemi olduğu anlaşıldı. 2013'te Nobel ödülünün olası en büyük adaylarından Feng Zhang ve Jennifer Doudna, CRISPR sistemini insan genetiğini değiştirmek hatta tamir edebilmek için optimize ettiğini duyurdu. Takip eden beş yıllık süreçte dünya üzerinde bu tekniği kullanarak insan, hayvan, bitki ve böcek olmak üzere birçok canlı türünün genetiğini başarıyla modifiye edebildiğini rapor eden binlerce bilimsel makale yayınlandı.","page_numbers":"46-47","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"Hospital Manager"},"translated_abstract":"2013 yılının ilk aylarında New York Üniversitesinde katıldığım bir derse ilaç şirketlerinden yöneticiler davet edilmişti. Hastalıklar üzerine ilaç geliştirme çalışmalarını anlattıktan sonra katılımcılardan dikkat çeken bir soru yöneldi: Afrika'da birçok hastalık mevcut ve daha bunlar üzerine hiçbir ilaç araçtırma – geliştirme çalışması yapılmamış. Neden ilaç şirketleri hastalık portföyünü ve ilaç yatırımlarını genişletmiyor? Yöneticinin verdiği cevap etik görünmese de şirketlerin hastalıklardan muzdarip insanlara yaklaşımını özetlediği için trajikti: Birçok hastalık için araştırma yatırımı yapılsa Afrika'daki bu hastalıklara da ilaçlar geliştirilebilir. Ancak satılacak bu ilaçların alıcılarının maddi imkanlarını ve satılabilecek ilaç miktarının küçüklüğünden ötürü birçok hastalık için herhangi bir çalışma gerçekleştirilmiyor. Bu durum özellikle genetik/ailesel/kalıtsal hastalıklar için kaçınılmaz bir gerçekti. Ta ki yine 2013 yılında kolay, ucuz ve yüksek ulaşılabilirlikte CRISPR genom modifikasyon tekniğinin geliştirilmesiyle, insan hücrelerinin genetiğinin tamir edilebileceğini gösteren bilimsel makaleler yayınlanıncaya dek. Bu vakitten sonra ilaç şirketleri AR-GE çalışmalarını genetik tedaviler üzerine yoğunlaştırmaya başladı. Birçok biyoteknoloji şirketi nadir ve halk arasında tedavi edilmesi olanaksız hastalıkların gen terapi metotlarıyla tamir etmek üzerine amansız bir yarışa atıldı. Aslına bakarsanız CRISPR genom modifikasyon mekanizması 1987'de bakteri genomunda keşfedildi ve tekrarlı DNA dizilerinden oluşmalarından ötürü CRISPR olarak adlandırıldı. Bu dizilerin tam olarak fonksiyonunun anlaşılması için yirmi yıl geçmesi gerekti ve 2007'de bakterilerin virüslere karşı sahip olduğu bağışıklık sistemi olduğu anlaşıldı. 2013'te Nobel ödülünün olası en büyük adaylarından Feng Zhang ve Jennifer Doudna, CRISPR sistemini insan genetiğini değiştirmek hatta tamir edebilmek için optimize ettiğini duyurdu. Takip eden beş yıllık süreçte dünya üzerinde bu tekniği kullanarak insan, hayvan, bitki ve böcek olmak üzere birçok canlı türünün genetiğini başarıyla modifiye edebildiğini rapor eden binlerce bilimsel makale yayınlandı.","internal_url":"https://www.academia.edu/36845381/Tamiri_%C4%B0mk%C3%A2ns%C4%B1z_San%C4%B1lan_Genetik_Hastal%C4%B1klara_Gen_Terapi_Olana%C4%9F%C4%B1_","translated_internal_url":"","created_at":"2018-06-14T13:45:53.678-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[],"downloadable_attachments":[{"id":56829839,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56829839/thumbnails/1.jpg","file_name":"CIHAN_TASTAN.pdf","download_url":"https://www.academia.edu/attachments/56829839/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Tamiri_Imkansiz_Sanilan_Genetik_Hastalik.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56829839/CIHAN_TASTAN-libre.pdf?1529484662=\u0026response-content-disposition=attachment%3B+filename%3DTamiri_Imkansiz_Sanilan_Genetik_Hastalik.pdf\u0026Expires=1732704105\u0026Signature=SugbpWt28W0N7-P6ZDHSz15mKBF65jjC8g5SjCU6Gbye6JngSK0wJA2nmHzjFSnGt73PeoUd8bV98glememvfz7SHBw3gsIqgIuHwupkJJfrPnyQCYOrGNSxOAZKc3tz7rUPO4IMhfN2piMI7xfa7T1lWZkVsz52vT0U5VFGLQrhoZQn~4wytYnUGbDOEfS3pRtPhQFi3WoqNQltEpy3UwKNnPunIDMeVUdeAc2A~bcZDou~xitOm1OpJh0Fi0s5nfpfloZUddt98HoA2Q6HY8hNJCgTrx19NWqWkgmBkFJckIOu-28BmC9H0lEJkeuFa7l~CgbG7adzfNq-mhtI~Q__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Tamiri_İmkânsız_Sanılan_Genetik_Hastalıklara_Gen_Terapi_Olanağı_","translated_slug":"","page_count":2,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"WG1IYzFjL01hSTFrOGxYb2hib1AzRkI5YmtvbzZZUVBLQjF4Y2pKdFRoamcrU1doYVNiR29HYjk1bjZtcmd2UC0tTHcyYmF3bHpXQVpjeTFjR2RYRzBiZz09--7a3656aa51aab0ea635c38b7e089d105ae37a8e9"},"attachments":[{"id":56829839,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56829839/thumbnails/1.jpg","file_name":"CIHAN_TASTAN.pdf","download_url":"https://www.academia.edu/attachments/56829839/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Tamiri_Imkansiz_Sanilan_Genetik_Hastalik.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56829839/CIHAN_TASTAN-libre.pdf?1529484662=\u0026response-content-disposition=attachment%3B+filename%3DTamiri_Imkansiz_Sanilan_Genetik_Hastalik.pdf\u0026Expires=1732704105\u0026Signature=SugbpWt28W0N7-P6ZDHSz15mKBF65jjC8g5SjCU6Gbye6JngSK0wJA2nmHzjFSnGt73PeoUd8bV98glememvfz7SHBw3gsIqgIuHwupkJJfrPnyQCYOrGNSxOAZKc3tz7rUPO4IMhfN2piMI7xfa7T1lWZkVsz52vT0U5VFGLQrhoZQn~4wytYnUGbDOEfS3pRtPhQFi3WoqNQltEpy3UwKNnPunIDMeVUdeAc2A~bcZDou~xitOm1OpJh0Fi0s5nfpfloZUddt98HoA2Q6HY8hNJCgTrx19NWqWkgmBkFJckIOu-28BmC9H0lEJkeuFa7l~CgbG7adzfNq-mhtI~Q__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1170194,"name":"Genetik Hastalıklar","url":"https://www.academia.edu/Documents/in/Genetik_Hastaliklar"},{"id":1614390,"name":"Biyokimya Bio Teknoloji Genetik Mühendisliği Genom Projeleri","url":"https://www.academia.edu/Documents/in/Biyokimya_Bio_Teknoloji_Genetik_Muhendisligi_Genom_Projeleri"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36499702"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36499702/Vir%C3%BCs_Benzeri_Haf%C4%B1za_Arc_Proteinleriyle_N%C3%B6rolojik_Hastal%C4%B1klara_Genetik_Tedavi_%C3%9Cmidi"><img alt="Research paper thumbnail of Virüs Benzeri Hafıza Arc Proteinleriyle Nörolojik Hastalıklara Genetik Tedavi Ümidi" class="work-thumbnail" src="https://attachments.academia-assets.com/56419720/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36499702/Vir%C3%BCs_Benzeri_Haf%C4%B1za_Arc_Proteinleriyle_N%C3%B6rolojik_Hastal%C4%B1klara_Genetik_Tedavi_%C3%9Cmidi">Virüs Benzeri Hafıza Arc Proteinleriyle Nörolojik Hastalıklara Genetik Tedavi Ümidi</a></div><div class="wp-workCard_item"><span>Bezelye Dergi</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Hafızada yer alan ve Alzheimer hastalığı gibi nörolojik rahatsızlıklara karışan bir genin davranı...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Hafızada yer alan ve Alzheimer hastalığı gibi nörolojik rahatsızlıklara karışan bir genin davranışını incelemek için yapı-lan araştırmalar beklenmedik bir şekilde nöronların genetik bilgi-yi  birbirlerine  göndermek  için  kullanabilecekleri  tamamen  yeni  bir mekanizmanın keşfedilmesine yol açtı.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9640282b229b2217edfbc4286ae56769" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56419720,"asset_id":36499702,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56419720/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36499702"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36499702"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36499702; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36322512"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36322512/Yerli_H%C3%BCcresel_Terapiler_T%C3%BCrkiye_Sa%C4%9Fl%C4%B1k_Turizmine_Yeni_Boyut_Kazand%C4%B1racak"><img alt="Research paper thumbnail of Yerli Hücresel Terapiler, Türkiye Sağlık Turizmine Yeni Boyut Kazandıracak" class="work-thumbnail" src="https://attachments.academia-assets.com/56229812/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36322512/Yerli_H%C3%BCcresel_Terapiler_T%C3%BCrkiye_Sa%C4%9Fl%C4%B1k_Turizmine_Yeni_Boyut_Kazand%C4%B1racak">Yerli Hücresel Terapiler, Türkiye Sağlık Turizmine Yeni Boyut Kazandıracak</a></div><div class="wp-workCard_item"><span>Biomedya </span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Türkiye, Orta Doğu ve Arap ülkeleri gibi Avrupa ülkelerinden gelen tıbbi turistler için de sıklık...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Türkiye, Orta Doğu ve Arap ülkeleri gibi Avrupa ülkelerinden gelen tıbbi turistler için de sıklıkla tercih edilen bir yer olmuştur. Türkiye'de bulunan birçok hastanenin JCI (Uluslararası Ortak Komisyonlar), JACHO (Sağlık Kuruluşları Akreditasyon Ortak Komisyonu), ISO (Uluslararası Standardizasyon Örgütü) dahil olmak üzere yerel ve uluslararası akreditasyonları bulunuyor. Türkiye özellikle bazı tıp alanlarında ABD ve birçok Avrupa ülkesine nazaran çok daha ucuz fiyatlarıyla sağlık turizminde  cezbedici durumda bulunuyor. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="35849410"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/35849410/Yeni_Nesil_%C4%B0la%C3%A7_End%C3%BCstrisinde_Genetik_M%C3%BChendisli%C4%9Finin_%C3%96nemi"><img alt="Research paper thumbnail of Yeni Nesil İlaç Endüstrisinde Genetik Mühendisliğinin Önemi" class="work-thumbnail" src="https://attachments.academia-assets.com/55727558/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/35849410/Yeni_Nesil_%C4%B0la%C3%A7_End%C3%BCstrisinde_Genetik_M%C3%BChendisli%C4%9Finin_%C3%96nemi">Yeni Nesil İlaç Endüstrisinde Genetik Mühendisliğinin Önemi</a></div><div class="wp-workCard_item"><span>Biomedya</span><span>, 2018</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2b94199c3c82439bd1baead2c3d176ce" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":55727558,"asset_id":35849410,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/55727558/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="35849410"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="35849410"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 35849410; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="35352615"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/35352615/Mikrobiyom_and_Probiyotik_Pazar%C4%B1_Biyoteknoloji_yi_10_Y%C4%B1l_%C4%B0%C3%A7inde_Yakalayacak_"><img alt="Research paper thumbnail of Mikrobiyom & Probiyotik Pazarı, Biyoteknoloji’yi 10 Yıl İçinde Yakalayacak!" class="work-thumbnail" src="https://attachments.academia-assets.com/55213001/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/35352615/Mikrobiyom_and_Probiyotik_Pazar%C4%B1_Biyoteknoloji_yi_10_Y%C4%B1l_%C4%B0%C3%A7inde_Yakalayacak_">Mikrobiyom & Probiyotik Pazarı, Biyoteknoloji’yi 10 Yıl İçinde Yakalayacak!</a></div><div class="wp-workCard_item"><span>Biomedya</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mikrobiyom, gıda ve aynı zamanda bulaşıcı hastalıklar üzerinde tamamen egemen olan kronik ve meta...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mikrobiyom, gıda ve aynı zamanda bulaşıcı hastalıklar üzerinde tamamen egemen olan kronik ve metabolik hastalıklarla bağlantılıdır. Bağırsak mikroplarının sekanslanması 2008'de uygun fiyatlı hale geldiğinde, bu alan patladı ve ‘mikrobiyom’ olarak markalaştı.<br /><br />Seventure Partners'ın CEO'su olan Isabelle de Cremoux, esasen mikrobiyoloji üzerine yoğunlaşıyor - bunu yapmak için 160 milyon avroluk bir fon sağladı. Mikrobiyom pazarının 10 yıl içinde Biyoteknoloji pazarını yakalayacağını öngörüyor ve şu tespitte bulunuyor: ‘CRISPR, CAR-T ve gen terapisi bir genç insan sayılırsa, mikrobiyom daha bebeklik döneminde’.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b8a5489d8afd8b5857a5223556df2888" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":55213001,"asset_id":35352615,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/55213001/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="35352615"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="35352615"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 35352615; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=35352615]").text(description); $(".js-view-count[data-work-id=35352615]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 35352615; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='35352615']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 35352615, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b8a5489d8afd8b5857a5223556df2888" } } $('.js-work-strip[data-work-id=35352615]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":35352615,"title":"Mikrobiyom \u0026 Probiyotik Pazarı, Biyoteknoloji’yi 10 Yıl İçinde Yakalayacak!","translated_title":"","metadata":{"abstract":"Mikrobiyom, gıda ve aynı zamanda bulaşıcı hastalıklar üzerinde tamamen egemen olan kronik ve metabolik hastalıklarla bağlantılıdır. Bağırsak mikroplarının sekanslanması 2008'de uygun fiyatlı hale geldiğinde, bu alan patladı ve ‘mikrobiyom’ olarak markalaştı.\n\nSeventure Partners'ın CEO'su olan Isabelle de Cremoux, esasen mikrobiyoloji üzerine yoğunlaşıyor - bunu yapmak için 160 milyon avroluk bir fon sağladı. Mikrobiyom pazarının 10 yıl içinde Biyoteknoloji pazarını yakalayacağını öngörüyor ve şu tespitte bulunuyor: ‘CRISPR, CAR-T ve gen terapisi bir genç insan sayılırsa, mikrobiyom daha bebeklik döneminde’. \n","publication_name":"Biomedya"},"translated_abstract":"Mikrobiyom, gıda ve aynı zamanda bulaşıcı hastalıklar üzerinde tamamen egemen olan kronik ve metabolik hastalıklarla bağlantılıdır. Bağırsak mikroplarının sekanslanması 2008'de uygun fiyatlı hale geldiğinde, bu alan patladı ve ‘mikrobiyom’ olarak markalaştı.\n\nSeventure Partners'ın CEO'su olan Isabelle de Cremoux, esasen mikrobiyoloji üzerine yoğunlaşıyor - bunu yapmak için 160 milyon avroluk bir fon sağladı. Mikrobiyom pazarının 10 yıl içinde Biyoteknoloji pazarını yakalayacağını öngörüyor ve şu tespitte bulunuyor: ‘CRISPR, CAR-T ve gen terapisi bir genç insan sayılırsa, mikrobiyom daha bebeklik döneminde’. \n","internal_url":"https://www.academia.edu/35352615/Mikrobiyom_and_Probiyotik_Pazar%C4%B1_Biyoteknoloji_yi_10_Y%C4%B1l_%C4%B0%C3%A7inde_Yakalayacak_","translated_internal_url":"","created_at":"2017-12-05T22:12:09.108-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":55213001,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/55213001/thumbnails/1.jpg","file_name":"BioMedya_11_2.pdf","download_url":"https://www.academia.edu/attachments/55213001/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Mikrobiyom_and_Probiyotik_Pazari_Biyotek.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/55213001/BioMedya_11_2-libre.pdf?1512545810=\u0026response-content-disposition=attachment%3B+filename%3DMikrobiyom_and_Probiyotik_Pazari_Biyotek.pdf\u0026Expires=1732739028\u0026Signature=aRjedvi23-vWua8zFJUC3O3jsXBraz6iblzbrBeQsraP0v66lUq~jM-FyPfIeNY4B5t6V5FJtSkNRh8SpNm43I2v3LSsQ8WrygOwtHD~7ekufNKjN5l5u67EAknPXzAgOITC7LSCuMuGtjxJvVi3xTonEAJWEI8aQKG0c79NQR2hgFQEwXCoC8w7GE9DkT0x1gXPYljR8-19Mq2ZhXTpts7yO1zsk3F2HqyobvRFClI~vmput8oOwW581lOuIiD8ZgBnGIU-D07Sl4sVulk~7J3fr~NIeHh~tlO6w-4zH0ghK07mZioiWF7e8Nfu~hFG3gFKarnAVdKRLs48sTIxAg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Mikrobiyom_and_Probiyotik_Pazarı_Biyoteknoloji_yi_10_Yıl_İçinde_Yakalayacak_","translated_slug":"","page_count":1,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":55213001,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/55213001/thumbnails/1.jpg","file_name":"BioMedya_11_2.pdf","download_url":"https://www.academia.edu/attachments/55213001/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Mikrobiyom_and_Probiyotik_Pazari_Biyotek.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/55213001/BioMedya_11_2-libre.pdf?1512545810=\u0026response-content-disposition=attachment%3B+filename%3DMikrobiyom_and_Probiyotik_Pazari_Biyotek.pdf\u0026Expires=1732739028\u0026Signature=aRjedvi23-vWua8zFJUC3O3jsXBraz6iblzbrBeQsraP0v66lUq~jM-FyPfIeNY4B5t6V5FJtSkNRh8SpNm43I2v3LSsQ8WrygOwtHD~7ekufNKjN5l5u67EAknPXzAgOITC7LSCuMuGtjxJvVi3xTonEAJWEI8aQKG0c79NQR2hgFQEwXCoC8w7GE9DkT0x1gXPYljR8-19Mq2ZhXTpts7yO1zsk3F2HqyobvRFClI~vmput8oOwW581lOuIiD8ZgBnGIU-D07Sl4sVulk~7J3fr~NIeHh~tlO6w-4zH0ghK07mZioiWF7e8Nfu~hFG3gFKarnAVdKRLs48sTIxAg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology"},{"id":159316,"name":"Mikrobiyoloji","url":"https://www.academia.edu/Documents/in/Mikrobiyoloji"},{"id":537754,"name":"Microbiota","url":"https://www.academia.edu/Documents/in/Microbiota"},{"id":976609,"name":"Probiotic Microbiology","url":"https://www.academia.edu/Documents/in/Probiotic_Microbiology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34089156"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34089156/_Kanser_%C4%B0mmunoterapi_de_ge%C3%A7mi%C5%9F_ve_gelecek"><img alt="Research paper thumbnail of "Kanser İmmunoterapi" de geçmiş ve gelecek" class="work-thumbnail" src="https://attachments.academia-assets.com/54022015/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34089156/_Kanser_%C4%B0mmunoterapi_de_ge%C3%A7mi%C5%9F_ve_gelecek">"Kanser İmmunoterapi" de geçmiş ve gelecek</a></div><div class="wp-workCard_item"><span>Biomedya</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">CAR-T hücre tedavisini Türkiye’de üretmek ve başarmak genetik bilimimizin uygulanmaya dönüştürülm...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">CAR-T hücre tedavisini Türkiye’de üretmek ve başarmak genetik bilimimizin uygulanmaya dönüştürülmesi ve biyoteknolojik tıp konusunda yerlileşme ve dışa bağımlılığımızı azaltma noktasında çok mühim. Bunun yanında ülkemiz sağlık turizminde Amerika ve diğer ülkelere kıyasla çok ucuz olmasından ötürü çekici bir özelliğe sahip. CAR-T hücre tedavisinin ucuza yerli imkanlarla gerçekleştirilmesi, bu tedavinin yüz binlerce doları bulduğu göz önüne alındığında sağlık turizminde elimizi güçlendirecektir.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="eb5c9216e076141d6b8d3d3572247cb7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54022015,"asset_id":34089156,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54022015/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34089156"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34089156"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34089156; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34089156]").text(description); $(".js-view-count[data-work-id=34089156]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34089156; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34089156']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34089156, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "eb5c9216e076141d6b8d3d3572247cb7" } } $('.js-work-strip[data-work-id=34089156]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34089156,"title":"\"Kanser İmmunoterapi\" de geçmiş ve gelecek","translated_title":"","metadata":{"abstract":"CAR-T hücre tedavisini Türkiye’de üretmek ve başarmak genetik bilimimizin uygulanmaya dönüştürülmesi ve biyoteknolojik tıp konusunda yerlileşme ve dışa bağımlılığımızı azaltma noktasında çok mühim. Bunun yanında ülkemiz sağlık turizminde Amerika ve diğer ülkelere kıyasla çok ucuz olmasından ötürü çekici bir özelliğe sahip. CAR-T hücre tedavisinin ucuza yerli imkanlarla gerçekleştirilmesi, bu tedavinin yüz binlerce doları bulduğu göz önüne alındığında sağlık turizminde elimizi güçlendirecektir.","publication_name":"Biomedya"},"translated_abstract":"CAR-T hücre tedavisini Türkiye’de üretmek ve başarmak genetik bilimimizin uygulanmaya dönüştürülmesi ve biyoteknolojik tıp konusunda yerlileşme ve dışa bağımlılığımızı azaltma noktasında çok mühim. Bunun yanında ülkemiz sağlık turizminde Amerika ve diğer ülkelere kıyasla çok ucuz olmasından ötürü çekici bir özelliğe sahip. CAR-T hücre tedavisinin ucuza yerli imkanlarla gerçekleştirilmesi, bu tedavinin yüz binlerce doları bulduğu göz önüne alındığında sağlık turizminde elimizi güçlendirecektir.","internal_url":"https://www.academia.edu/34089156/_Kanser_%C4%B0mmunoterapi_de_ge%C3%A7mi%C5%9F_ve_gelecek","translated_internal_url":"","created_at":"2017-07-31T09:16:56.532-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":54022015,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54022015/thumbnails/1.jpg","file_name":"document_2.pdf","download_url":"https://www.academia.edu/attachments/54022015/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Kanser_Immunoterapi_de_gecmis_ve_gelece.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54022015/document_2-libre.pdf?1501518354=\u0026response-content-disposition=attachment%3B+filename%3DKanser_Immunoterapi_de_gecmis_ve_gelece.pdf\u0026Expires=1732739028\u0026Signature=DVvBeYRNoclfwd0iHK14CAm0rAqwH1GbNoaPIVE4MlSlut2odJ1IORyqrC6J7DCSgHXkbIVf-DidWf9WMCsd46mqCWE8LbkPq0SvywGxHa1aTwUEoRl52bwt9fbGcakTzJ3TWfLaGXjm59hF19wJg2QMWeaB-1cs3BIbXJzUQAkPLkfM9zv0OePUFwu8LCil0yrUa-YxMQFoRgBpN6HVDKTDKapuoNk91GzjWfNQTiqXD1ukCb5Km9yJVmThdbGcFMe1xNDXY5f4d0UlLIS3GVP7L3tFAHn5JAzuWtVgUq3Yvak8LTcsvglNe7zs-rQ8~QbuL9FmjXmEgM9AY8RfgQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"_Kanser_İmmunoterapi_de_geçmiş_ve_gelecek","translated_slug":"","page_count":2,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":54022015,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54022015/thumbnails/1.jpg","file_name":"document_2.pdf","download_url":"https://www.academia.edu/attachments/54022015/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Kanser_Immunoterapi_de_gecmis_ve_gelece.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54022015/document_2-libre.pdf?1501518354=\u0026response-content-disposition=attachment%3B+filename%3DKanser_Immunoterapi_de_gecmis_ve_gelece.pdf\u0026Expires=1732739028\u0026Signature=DVvBeYRNoclfwd0iHK14CAm0rAqwH1GbNoaPIVE4MlSlut2odJ1IORyqrC6J7DCSgHXkbIVf-DidWf9WMCsd46mqCWE8LbkPq0SvywGxHa1aTwUEoRl52bwt9fbGcakTzJ3TWfLaGXjm59hF19wJg2QMWeaB-1cs3BIbXJzUQAkPLkfM9zv0OePUFwu8LCil0yrUa-YxMQFoRgBpN6HVDKTDKapuoNk91GzjWfNQTiqXD1ukCb5Km9yJVmThdbGcFMe1xNDXY5f4d0UlLIS3GVP7L3tFAHn5JAzuWtVgUq3Yvak8LTcsvglNe7zs-rQ8~QbuL9FmjXmEgM9AY8RfgQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":22103,"name":"Cancer Immunotherapy","url":"https://www.academia.edu/Documents/in/Cancer_Immunotherapy"},{"id":895498,"name":"SAĞLIK ALTERNATİF KANSER İMMÜN SİSTEM KUVVETLENDİRİCİ","url":"https://www.academia.edu/Documents/in/SAGLIK_ALTERNATIF_KANSER_IMMUN_SISTEM_KUVVETLENDIRICI"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="33366663"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/33366663/Kanseri_Yok_Edebilen_Yapay_Organ"><img alt="Research paper thumbnail of Kanseri Yok Edebilen Yapay Organ" class="work-thumbnail" src="https://attachments.academia-assets.com/53423558/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/33366663/Kanseri_Yok_Edebilen_Yapay_Organ">Kanseri Yok Edebilen Yapay Organ</a></div><div class="wp-workCard_item"><span>BioMedya</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Biyomedikal teknolojilerdeki son gelişmelerle, nakil isteyenler artık böbrek ve kan damarları gib...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Biyomedikal teknolojilerdeki son<br />gelişmelerle, nakil isteyenler artık<br />böbrek ve kan damarları gibi organları<br />değiştirmek için bağış listelerinde<br />sıralarını beklemek zorunda<br />kalmayabilirler. Bu geliştirilmekte<br />olan sentetik organların içerisine<br />artık bağışıklık sistemi hücrelerini<br />üretebilecek yapay timüs organları da<br />dahil oldu.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e89395d0e0f64338de0a25aedda7cb73" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":53423558,"asset_id":33366663,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/53423558/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="33366663"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="33366663"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 33366663; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=33366663]").text(description); $(".js-view-count[data-work-id=33366663]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 33366663; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='33366663']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 33366663, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e89395d0e0f64338de0a25aedda7cb73" } } $('.js-work-strip[data-work-id=33366663]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":33366663,"title":"Kanseri Yok Edebilen Yapay Organ","translated_title":"","metadata":{"abstract":"Biyomedikal teknolojilerdeki son\ngelişmelerle, nakil isteyenler artık\nböbrek ve kan damarları gibi organları\ndeğiştirmek için bağış listelerinde\nsıralarını beklemek zorunda\nkalmayabilirler. Bu geliştirilmekte\nolan sentetik organların içerisine\nartık bağışıklık sistemi hücrelerini\nüretebilecek yapay timüs organları da\ndahil oldu.","publication_name":"BioMedya"},"translated_abstract":"Biyomedikal teknolojilerdeki son\ngelişmelerle, nakil isteyenler artık\nböbrek ve kan damarları gibi organları\ndeğiştirmek için bağış listelerinde\nsıralarını beklemek zorunda\nkalmayabilirler. Bu geliştirilmekte\nolan sentetik organların içerisine\nartık bağışıklık sistemi hücrelerini\nüretebilecek yapay timüs organları da\ndahil oldu.","internal_url":"https://www.academia.edu/33366663/Kanseri_Yok_Edebilen_Yapay_Organ","translated_internal_url":"","created_at":"2017-06-07T09:49:52.765-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":53423558,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/53423558/thumbnails/1.jpg","file_name":"document.pdf","download_url":"https://www.academia.edu/attachments/53423558/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Kanseri_Yok_Edebilen_Yapay_Organ.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/53423558/document-libre.pdf?1496854309=\u0026response-content-disposition=attachment%3B+filename%3DKanseri_Yok_Edebilen_Yapay_Organ.pdf\u0026Expires=1732739028\u0026Signature=Etxjq660pMMFRPGudrdvBo~XoObc39CcNvsH3RyFGUVXr6ingYbdYLZqLgnUxfioGYiayBdaIOrm92c2R6UGpnoeLh1UKe7JBP~bfEhTL~0~tcy7-3vzKrkvIJIWmCQUxMY8VcoECpRI4HqB4q9NfH-Na5bY8zzHa9-5NPw9o2Uv9IIEdFEEGN9GalQD3GvaZ9UPdzUvVf~8ifZKzupGgJtVwC8Rpf8yQepSy7Hmr5KcuayBbBqiRPeZ3hKSR5Q5TVIEofBbitomiOASTezuS3DuaQK1HuWpC9G2aOL78PoJkAHX-7JEHyvrKjdDbb2~OO9KsN9wZtIEXHwUJErw3w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Kanseri_Yok_Edebilen_Yapay_Organ","translated_slug":"","page_count":1,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":53423558,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/53423558/thumbnails/1.jpg","file_name":"document.pdf","download_url":"https://www.academia.edu/attachments/53423558/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Kanseri_Yok_Edebilen_Yapay_Organ.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/53423558/document-libre.pdf?1496854309=\u0026response-content-disposition=attachment%3B+filename%3DKanseri_Yok_Edebilen_Yapay_Organ.pdf\u0026Expires=1732739028\u0026Signature=Etxjq660pMMFRPGudrdvBo~XoObc39CcNvsH3RyFGUVXr6ingYbdYLZqLgnUxfioGYiayBdaIOrm92c2R6UGpnoeLh1UKe7JBP~bfEhTL~0~tcy7-3vzKrkvIJIWmCQUxMY8VcoECpRI4HqB4q9NfH-Na5bY8zzHa9-5NPw9o2Uv9IIEdFEEGN9GalQD3GvaZ9UPdzUvVf~8ifZKzupGgJtVwC8Rpf8yQepSy7Hmr5KcuayBbBqiRPeZ3hKSR5Q5TVIEofBbitomiOASTezuS3DuaQK1HuWpC9G2aOL78PoJkAHX-7JEHyvrKjdDbb2~OO9KsN9wZtIEXHwUJErw3w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":122648,"name":"Kök Hücre","url":"https://www.academia.edu/Documents/in/Kok_Hucre"},{"id":775604,"name":"Kanser Tedavisi","url":"https://www.academia.edu/Documents/in/Kanser_Tedavisi"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="32720582"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/32720582/Nadir_Hastal%C4%B1klara_Umut_olan_CRISPR_Gen_Terapisi"><img alt="Research paper thumbnail of Nadir Hastalıklara Umut olan CRISPR Gen Terapisi" class="work-thumbnail" src="https://attachments.academia-assets.com/52880628/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/32720582/Nadir_Hastal%C4%B1klara_Umut_olan_CRISPR_Gen_Terapisi">Nadir Hastalıklara Umut olan CRISPR Gen Terapisi</a></div><div class="wp-workCard_item"><span>Bezelye dergi</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Dünya üzerinde tahmini 300-350 milyon nadir hastalığa sahip insanın bulunmasına rağmen, hastalık ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Dünya üzerinde tahmini 300-350 milyon nadir hastalığa sahip insanın bulunmasına rağmen, hastalık türlerinin %95'i için hala tedavi edici onaylı bir ilaç bulunmuyor. Hali hazırda satılmakta olan ilaçların da çok pahalı olması, ilaç şirketlerinin bu alana itibar etmemesinin başlıca nedenleri arasında sayılıyordu. Ta ki son birkaç yıla dek.<br /><br />Halk arasında ‘genetik mühendisliği’ olarak bilinen genetik çalışmalar, ‘tedavi edilmesi olanaksız’ görülen genetik tabanlı hastalıklar için artık bir umut ışığı olmaya başladı.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fe93a02d5c37c1ce6c08b67976b88dc9" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":52880628,"asset_id":32720582,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/52880628/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="32720582"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="32720582"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 32720582; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=32720582]").text(description); $(".js-view-count[data-work-id=32720582]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 32720582; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='32720582']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 32720582, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fe93a02d5c37c1ce6c08b67976b88dc9" } } $('.js-work-strip[data-work-id=32720582]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":32720582,"title":"Nadir Hastalıklara Umut olan CRISPR Gen Terapisi","translated_title":"","metadata":{"abstract":"Dünya üzerinde tahmini 300-350 milyon nadir hastalığa sahip insanın bulunmasına rağmen, hastalık türlerinin %95'i için hala tedavi edici onaylı bir ilaç bulunmuyor. Hali hazırda satılmakta olan ilaçların da çok pahalı olması, ilaç şirketlerinin bu alana itibar etmemesinin başlıca nedenleri arasında sayılıyordu. Ta ki son birkaç yıla dek.\n\nHalk arasında ‘genetik mühendisliği’ olarak bilinen genetik çalışmalar, ‘tedavi edilmesi olanaksız’ görülen genetik tabanlı hastalıklar için artık bir umut ışığı olmaya başladı.\n","publication_name":"Bezelye dergi"},"translated_abstract":"Dünya üzerinde tahmini 300-350 milyon nadir hastalığa sahip insanın bulunmasına rağmen, hastalık türlerinin %95'i için hala tedavi edici onaylı bir ilaç bulunmuyor. Hali hazırda satılmakta olan ilaçların da çok pahalı olması, ilaç şirketlerinin bu alana itibar etmemesinin başlıca nedenleri arasında sayılıyordu. Ta ki son birkaç yıla dek.\n\nHalk arasında ‘genetik mühendisliği’ olarak bilinen genetik çalışmalar, ‘tedavi edilmesi olanaksız’ görülen genetik tabanlı hastalıklar için artık bir umut ışığı olmaya başladı.\n","internal_url":"https://www.academia.edu/32720582/Nadir_Hastal%C4%B1klara_Umut_olan_CRISPR_Gen_Terapisi","translated_internal_url":"","created_at":"2017-04-29T14:55:32.663-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":52880628,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/52880628/thumbnails/1.jpg","file_name":"Nadir_Hastaliklara_CRISPR.pdf","download_url":"https://www.academia.edu/attachments/52880628/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Nadir_Hastaliklara_Umut_olan_CRISPR_Gen.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/52880628/Nadir_Hastaliklara_CRISPR-libre.pdf?1493503057=\u0026response-content-disposition=attachment%3B+filename%3DNadir_Hastaliklara_Umut_olan_CRISPR_Gen.pdf\u0026Expires=1732739028\u0026Signature=BzFMXo59J3qYsjyPonTxWTiIcX~DWgt8a-1p29AgXNlUNm5X5ltR~GZtWwWmU6DXFuwOhAhCHCovz-yrNLdYlNB3BViQaOQg~mmUWE~38mjJ1HsXR05HBXDUAZGxl2pzkSRkJbCZR6RPjlTO-A1GuJsnKVg-VBCauprkbvEGl8juTfvDOy1PoOor-HsIergu-Qc-PW2aTeg2NkD20TubJLT7gnPKCeux49SADxxMRM1jfK3cBJDGvTtmKO~rzfmwZ66o10vmfGVSUgLmt-T1hlDOlDQD-pcHOdNSXI878Qq9l0QHRymhHHb3a3E~4tsuf0fV-zLQ4akAenS3ozd8Ag__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Nadir_Hastalıklara_Umut_olan_CRISPR_Gen_Terapisi","translated_slug":"","page_count":1,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":52880628,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/52880628/thumbnails/1.jpg","file_name":"Nadir_Hastaliklara_CRISPR.pdf","download_url":"https://www.academia.edu/attachments/52880628/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Nadir_Hastaliklara_Umut_olan_CRISPR_Gen.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/52880628/Nadir_Hastaliklara_CRISPR-libre.pdf?1493503057=\u0026response-content-disposition=attachment%3B+filename%3DNadir_Hastaliklara_Umut_olan_CRISPR_Gen.pdf\u0026Expires=1732739028\u0026Signature=BzFMXo59J3qYsjyPonTxWTiIcX~DWgt8a-1p29AgXNlUNm5X5ltR~GZtWwWmU6DXFuwOhAhCHCovz-yrNLdYlNB3BViQaOQg~mmUWE~38mjJ1HsXR05HBXDUAZGxl2pzkSRkJbCZR6RPjlTO-A1GuJsnKVg-VBCauprkbvEGl8juTfvDOy1PoOor-HsIergu-Qc-PW2aTeg2NkD20TubJLT7gnPKCeux49SADxxMRM1jfK3cBJDGvTtmKO~rzfmwZ66o10vmfGVSUgLmt-T1hlDOlDQD-pcHOdNSXI878Qq9l0QHRymhHHb3a3E~4tsuf0fV-zLQ4akAenS3ozd8Ag__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":22379,"name":"Rare diseases","url":"https://www.academia.edu/Documents/in/Rare_diseases"},{"id":70125,"name":"CRISPR","url":"https://www.academia.edu/Documents/in/CRISPR"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="32280769"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/32280769/Hedef_Doku_ve_H%C3%BCcrelere_Gen_Terapi_Sistemlerinin_Ta%C5%9F%C4%B1nma_Teknolojileri"><img alt="Research paper thumbnail of Hedef Doku ve Hücrelere Gen Terapi Sistemlerinin Taşınma Teknolojileri" class="work-thumbnail" src="https://attachments.academia-assets.com/52497701/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/32280769/Hedef_Doku_ve_H%C3%BCcrelere_Gen_Terapi_Sistemlerinin_Ta%C5%9F%C4%B1nma_Teknolojileri">Hedef Doku ve Hücrelere Gen Terapi Sistemlerinin Taşınma Teknolojileri</a></div><div class="wp-workCard_item"><span>Biomedya</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Yakın zamanda CRISPR-Cas9 genom modifikasyon teknolojisinin geliştirilmesiyle, biyomedikal araştı...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Yakın zamanda CRISPR-Cas9 genom modifikasyon teknolojisinin geliştirilmesiyle, biyomedikal araştırma, ilaç keşfi ve geliştirme, hatta gen terapisi için değerli bir teknoloji olmaya başladı.<br /><br />Bununla birlikte, bu gibi sistemlerin her biri hücrelere etkili bir şekilde girebilmek ve işlevlerini yerine getirebilmek için verimli ve güvenli taşıma teknolojilerine ihtiyaç duyuyor. Gen terapilerinde hücre içerisine bu makromoleküllerin taşınması veya enjekte edilebilmesi için viral ve viral olmayan paketleme yöntemleri üzerinde çalışmalar olanca hızıyla devam ediyor.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0d460a16ee21a8ad13d01580dd26b84d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":52497701,"asset_id":32280769,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/52497701/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="32280769"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="32280769"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 32280769; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=32280769]").text(description); $(".js-view-count[data-work-id=32280769]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 32280769; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='32280769']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 32280769, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0d460a16ee21a8ad13d01580dd26b84d" } } $('.js-work-strip[data-work-id=32280769]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":32280769,"title":"Hedef Doku ve Hücrelere Gen Terapi Sistemlerinin Taşınma Teknolojileri","translated_title":"","metadata":{"abstract":"Yakın zamanda CRISPR-Cas9 genom modifikasyon teknolojisinin geliştirilmesiyle, biyomedikal araştırma, ilaç keşfi ve geliştirme, hatta gen terapisi için değerli bir teknoloji olmaya başladı.\n\nBununla birlikte, bu gibi sistemlerin her biri hücrelere etkili bir şekilde girebilmek ve işlevlerini yerine getirebilmek için verimli ve güvenli taşıma teknolojilerine ihtiyaç duyuyor. Gen terapilerinde hücre içerisine bu makromoleküllerin taşınması veya enjekte edilebilmesi için viral ve viral olmayan paketleme yöntemleri üzerinde çalışmalar olanca hızıyla devam ediyor.","publication_name":"Biomedya"},"translated_abstract":"Yakın zamanda CRISPR-Cas9 genom modifikasyon teknolojisinin geliştirilmesiyle, biyomedikal araştırma, ilaç keşfi ve geliştirme, hatta gen terapisi için değerli bir teknoloji olmaya başladı.\n\nBununla birlikte, bu gibi sistemlerin her biri hücrelere etkili bir şekilde girebilmek ve işlevlerini yerine getirebilmek için verimli ve güvenli taşıma teknolojilerine ihtiyaç duyuyor. Gen terapilerinde hücre içerisine bu makromoleküllerin taşınması veya enjekte edilebilmesi için viral ve viral olmayan paketleme yöntemleri üzerinde çalışmalar olanca hızıyla devam ediyor.","internal_url":"https://www.academia.edu/32280769/Hedef_Doku_ve_H%C3%BCcrelere_Gen_Terapi_Sistemlerinin_Ta%C5%9F%C4%B1nma_Teknolojileri","translated_internal_url":"","created_at":"2017-04-05T13:42:00.000-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":52497701,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/52497701/thumbnails/1.jpg","file_name":"BioMedya_7._Sayi.pdf","download_url":"https://www.academia.edu/attachments/52497701/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Hedef_Doku_ve_Hucrelere_Gen_Terapi_Siste.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/52497701/BioMedya_7._Sayi-libre.pdf?1491425801=\u0026response-content-disposition=attachment%3B+filename%3DHedef_Doku_ve_Hucrelere_Gen_Terapi_Siste.pdf\u0026Expires=1732739028\u0026Signature=XovjZLkBWnyHIJYb3E5jfJOLWwxWlI5od1jmVqzY4xUaAbwJabeEvUGiXFd-J5e6W~BadgSz38DmznYM27UZD8RsM2CAcp6oQEbdJDE91z43RZ~Ap8Ymxg0N~srWBBbnatV8Jlu~vcdWWdNWMkSHPSEp6l6i1O63bCHOvXJsywixEBEYIizDCl8wdT4P-m7za7St-I8PgEOzTjMwPg5-sAud5OZTaTAMTdK4ZFzceQq-lyntm0l~6pqgzsysYYF~VFz0tFujfwdB63wofDU29kYMFRDPBqXX4xwMdhu~ZmPk1SU4v8R6oy-tgsfNXZGAgXzs1Dpap83C~ehUmxdDag__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Hedef_Doku_ve_Hücrelere_Gen_Terapi_Sistemlerinin_Taşınma_Teknolojileri","translated_slug":"","page_count":1,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":52497701,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/52497701/thumbnails/1.jpg","file_name":"BioMedya_7._Sayi.pdf","download_url":"https://www.academia.edu/attachments/52497701/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Hedef_Doku_ve_Hucrelere_Gen_Terapi_Siste.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/52497701/BioMedya_7._Sayi-libre.pdf?1491425801=\u0026response-content-disposition=attachment%3B+filename%3DHedef_Doku_ve_Hucrelere_Gen_Terapi_Siste.pdf\u0026Expires=1732739029\u0026Signature=MJuxma6q-NQBJ5IVV~CunBDMeR4U60qo0Ea5znMpHKNfsANELHVpd0suIUAPWhvWLhIZiRa-EydTCHEurXO14NCxROAG12xZ6BSfvhgu1n-94sRb1IETbWKh9Rd7Nx50S-6X0wpGYXOWHSN9PUwLMWXDcrLRU0l7ewbBXeuvOuJ0wHlscsD5hXfIU-aEUG108QwvZhAL0c7-Lwn7HQukZcjxh46DRRapg4QG0z4u5jGZsH4k-dc8kq9JqCkJsanQIHGKwK~HQj~UZ5BrvNbPDSkdxLqu~lfNsCgvkc2EvoHX~R1vfjblu8wJjt4H6FIOL8jfj16bPuDaWRFBwAI7IQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy"},{"id":1916835,"name":"CRISPR-Cas9 system","url":"https://www.academia.edu/Documents/in/CRISPR-Cas9_system"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="32194886"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/32194886/PD_1_Geni_Hedefli_CRISPR_Modifikasyonu_ile_Kanseri_Yenebilen_Savas_c_%C4%B1_Hu_creler"><img alt="Research paper thumbnail of PD-1 Geni Hedefli CRISPR Modifikasyonu ile Kanseri Yenebilen Savaşçı Hücreler" class="work-thumbnail" src="https://attachments.academia-assets.com/52426984/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/32194886/PD_1_Geni_Hedefli_CRISPR_Modifikasyonu_ile_Kanseri_Yenebilen_Savas_c_%C4%B1_Hu_creler">PD-1 Geni Hedefli CRISPR Modifikasyonu ile Kanseri Yenebilen Savaşçı Hücreler</a></div><div class="wp-workCard_item"><span>Biomedya</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Son iki yılda neredeyse biyoloji laboratuvarlarının tümünü kasıp kavuran CRISPR genom modifikasyo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Son iki yılda neredeyse biyoloji laboratuvarlarının tümünü kasıp kavuran CRISPR genom modifikasyon tekniği, artık yeni bir aşamaya geçmiş bulunmakta. Bakterilerin virüslere (bakteriyofaj) karşı bağışıklık sistemi olarak kullandığı CRISPR sistemi, bakteriye bulaşan virüslerin DNA'sını tanıyarak mutasyonlar oluşturmasına dayanıyor. Böylelikle virüsün çoğalmasına, büyümesine veya başka bakterilere bulaşmasına olanak sağlayan genlerinde oluşan kalıcı hasar, bakteriye bulaşan virüsleri inaktif hale getirilebiliyor. Bu özelliğin modifiye dilerek geliştirilmesi sonucu, CRISPR tekniği ile artık birçok hücre türünde genom modifikasyonu yapabiliyoruz. CRISPR metoduyla hedeflenilen genler, DNA'nın diğer bölgelerine zarar verilmeden, mutasyon oluşturularak inaktif hale getirilebiliyor (knockout , deletion mutation). Hali hazırda CRISPR alanında hakimiyetini sürdüren Çin'li araştırmacılar, bilhassa kanser immunoterapi'de de bu tekniği insanlar üzerinde ilk kullanan insanlar olmayı başardılar. Onkolojist Lu You ve ekibinin gerçekleştirdiği çalışmada, kanserli hastadan izole ettikleri kendi bağışıklık sistemi hücrelerini CRISPR sistemi ile modifiye ettiler. Daha sonra, tekrar hastaya naklederek kanserli hücrelere karşı daha etkili bir saldırı gerçekleştirmeyi amaçlamaktalar. Metastatik akciğer kanseri olan hasta üzerinde denenen terapi yöntemi şu an için klinik aşamada. Yine de, kanser immunoterapi alanında ilaçlar yerine kullanılacak alternatif bir yöntem olmasından dolayı dikkatle takip ediliyor.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e74b55281dc61738b6ed5da1f5490b5d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":52426984,"asset_id":32194886,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/52426984/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="32194886"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="32194886"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 32194886; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=32194886]").text(description); $(".js-view-count[data-work-id=32194886]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 32194886; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='32194886']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 32194886, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e74b55281dc61738b6ed5da1f5490b5d" } } $('.js-work-strip[data-work-id=32194886]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":32194886,"title":"PD-1 Geni Hedefli CRISPR Modifikasyonu ile Kanseri Yenebilen Savaşçı Hücreler","translated_title":"","metadata":{"abstract":"Son iki yılda neredeyse biyoloji laboratuvarlarının tümünü kasıp kavuran CRISPR genom modifikasyon tekniği, artık yeni bir aşamaya geçmiş bulunmakta. Bakterilerin virüslere (bakteriyofaj) karşı bağışıklık sistemi olarak kullandığı CRISPR sistemi, bakteriye bulaşan virüslerin DNA'sını tanıyarak mutasyonlar oluşturmasına dayanıyor. Böylelikle virüsün çoğalmasına, büyümesine veya başka bakterilere bulaşmasına olanak sağlayan genlerinde oluşan kalıcı hasar, bakteriye bulaşan virüsleri inaktif hale getirilebiliyor. Bu özelliğin modifiye dilerek geliştirilmesi sonucu, CRISPR tekniği ile artık birçok hücre türünde genom modifikasyonu yapabiliyoruz. CRISPR metoduyla hedeflenilen genler, DNA'nın diğer bölgelerine zarar verilmeden, mutasyon oluşturularak inaktif hale getirilebiliyor (knockout , deletion mutation). Hali hazırda CRISPR alanında hakimiyetini sürdüren Çin'li araştırmacılar, bilhassa kanser immunoterapi'de de bu tekniği insanlar üzerinde ilk kullanan insanlar olmayı başardılar. Onkolojist Lu You ve ekibinin gerçekleştirdiği çalışmada, kanserli hastadan izole ettikleri kendi bağışıklık sistemi hücrelerini CRISPR sistemi ile modifiye ettiler. Daha sonra, tekrar hastaya naklederek kanserli hücrelere karşı daha etkili bir saldırı gerçekleştirmeyi amaçlamaktalar. Metastatik akciğer kanseri olan hasta üzerinde denenen terapi yöntemi şu an için klinik aşamada. Yine de, kanser immunoterapi alanında ilaçlar yerine kullanılacak alternatif bir yöntem olmasından dolayı dikkatle takip ediliyor.","publication_name":"Biomedya"},"translated_abstract":"Son iki yılda neredeyse biyoloji laboratuvarlarının tümünü kasıp kavuran CRISPR genom modifikasyon tekniği, artık yeni bir aşamaya geçmiş bulunmakta. Bakterilerin virüslere (bakteriyofaj) karşı bağışıklık sistemi olarak kullandığı CRISPR sistemi, bakteriye bulaşan virüslerin DNA'sını tanıyarak mutasyonlar oluşturmasına dayanıyor. Böylelikle virüsün çoğalmasına, büyümesine veya başka bakterilere bulaşmasına olanak sağlayan genlerinde oluşan kalıcı hasar, bakteriye bulaşan virüsleri inaktif hale getirilebiliyor. Bu özelliğin modifiye dilerek geliştirilmesi sonucu, CRISPR tekniği ile artık birçok hücre türünde genom modifikasyonu yapabiliyoruz. CRISPR metoduyla hedeflenilen genler, DNA'nın diğer bölgelerine zarar verilmeden, mutasyon oluşturularak inaktif hale getirilebiliyor (knockout , deletion mutation). Hali hazırda CRISPR alanında hakimiyetini sürdüren Çin'li araştırmacılar, bilhassa kanser immunoterapi'de de bu tekniği insanlar üzerinde ilk kullanan insanlar olmayı başardılar. Onkolojist Lu You ve ekibinin gerçekleştirdiği çalışmada, kanserli hastadan izole ettikleri kendi bağışıklık sistemi hücrelerini CRISPR sistemi ile modifiye ettiler. Daha sonra, tekrar hastaya naklederek kanserli hücrelere karşı daha etkili bir saldırı gerçekleştirmeyi amaçlamaktalar. Metastatik akciğer kanseri olan hasta üzerinde denenen terapi yöntemi şu an için klinik aşamada. Yine de, kanser immunoterapi alanında ilaçlar yerine kullanılacak alternatif bir yöntem olmasından dolayı dikkatle takip ediliyor.","internal_url":"https://www.academia.edu/32194886/PD_1_Geni_Hedefli_CRISPR_Modifikasyonu_ile_Kanseri_Yenebilen_Savas_c_%C4%B1_Hu_creler","translated_internal_url":"","created_at":"2017-04-01T23:27:08.723-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":52426984,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/52426984/thumbnails/1.jpg","file_name":"CRISPR_PD-1_Deletion_Immunoterapy.pdf","download_url":"https://www.academia.edu/attachments/52426984/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"PD_1_Geni_Hedefli_CRISPR_Modifikasyonu_i.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/52426984/CRISPR_PD-1_Deletion_Immunoterapy-libre.pdf?1491114886=\u0026response-content-disposition=attachment%3B+filename%3DPD_1_Geni_Hedefli_CRISPR_Modifikasyonu_i.pdf\u0026Expires=1732739029\u0026Signature=HnEsqfJ-NDdKJBDkHSUSWVoZ4LV0fjNxaWNW-rOLHZtf9Fs12ElXJbtC3tA0TzQKUIycyKilrz72sqNJz4eoAo5MrWKc0ESw7wc75SDrgP7bQjF5gQ8Nvvc8tIWr~Z5~3XMG3luhniasJjDFvPYj~fisqDRQbpKntHBrjPmT1mCkFU-w4LD5Tpp61MYJVPLC43wP6vzbSqERrbQ72sQiZMxMvnwYqdcmtCiI0WZ34rDSEyqrOVi8FXTWfdobiOT0G14mLdm~GqJTekqjHOyu1dwuOTGaMfhIv-QHtGaG1lEZJFFbOcZ8hzFV7OmgVJ0-3ntOfbLz~QQ-q8lSpe5ZGA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"PD_1_Geni_Hedefli_CRISPR_Modifikasyonu_ile_Kanseri_Yenebilen_Savas_c_ı_Hu_creler","translated_slug":"","page_count":2,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":52426984,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/52426984/thumbnails/1.jpg","file_name":"CRISPR_PD-1_Deletion_Immunoterapy.pdf","download_url":"https://www.academia.edu/attachments/52426984/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"PD_1_Geni_Hedefli_CRISPR_Modifikasyonu_i.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/52426984/CRISPR_PD-1_Deletion_Immunoterapy-libre.pdf?1491114886=\u0026response-content-disposition=attachment%3B+filename%3DPD_1_Geni_Hedefli_CRISPR_Modifikasyonu_i.pdf\u0026Expires=1732739029\u0026Signature=HnEsqfJ-NDdKJBDkHSUSWVoZ4LV0fjNxaWNW-rOLHZtf9Fs12ElXJbtC3tA0TzQKUIycyKilrz72sqNJz4eoAo5MrWKc0ESw7wc75SDrgP7bQjF5gQ8Nvvc8tIWr~Z5~3XMG3luhniasJjDFvPYj~fisqDRQbpKntHBrjPmT1mCkFU-w4LD5Tpp61MYJVPLC43wP6vzbSqERrbQ72sQiZMxMvnwYqdcmtCiI0WZ34rDSEyqrOVi8FXTWfdobiOT0G14mLdm~GqJTekqjHOyu1dwuOTGaMfhIv-QHtGaG1lEZJFFbOcZ8hzFV7OmgVJ0-3ntOfbLz~QQ-q8lSpe5ZGA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":22103,"name":"Cancer Immunotherapy","url":"https://www.academia.edu/Documents/in/Cancer_Immunotherapy"},{"id":1916835,"name":"CRISPR-Cas9 system","url":"https://www.academia.edu/Documents/in/CRISPR-Cas9_system"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="32046939"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/32046939/Biyoendu_stri_2_0_CRISPR_Genom_Modifikasyonu"><img alt="Research paper thumbnail of Biyoendüstri 2.0: CRISPR Genom Modifikasyonu" class="work-thumbnail" src="https://attachments.academia-assets.com/52308442/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/32046939/Biyoendu_stri_2_0_CRISPR_Genom_Modifikasyonu">Biyoendüstri 2.0: CRISPR Genom Modifikasyonu</a></div><div class="wp-workCard_item"><span>Popular Science-Türkiye</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">18. yüzyılı takiben artan bilimsel gelişmeler ve buhar gücüyle çalışan makinelerin artması, dünya...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">18. yüzyılı takiben artan bilimsel gelişmeler ve buhar gücüyle çalışan makinelerin artması, dünya-mızda sanayi devrimi olarak adlandırdığımız yeni bir çağın başlamasına sebep oldu. Makinelerin gelişmesi ve bilgisayarın hayatımızın her anına daha etkin nü-fuz etmesiyle artık Endüstri 4.0'ı yaşamaya başladık. Biyoloji ve genetik alanında, her ne kadar aynı zaman dilimi içerisinde bilimsel çalışmalar yapılsa da Biyoendüstri diye adlandırabileceğimiz bir çağın ikinci versiyonuna geçmek için 2000'li yılları beklemek zorunda kaldık. Zira canlıları daha iyi anlayabilmek ve onlar-dan beklentilerimizi de yönlendirebilmek için mikro dünyaya inebilmemiz ve etki etme potansiyelimizi arttırabilmemiz gerekmekteydi. 2001'de üç milyar harften oluşan insan genomu tamamıyla deşifre edildiğinde çok az insan bunun aslında başlangıç olduğunu öngörüyordu. Çünkü DNA kitabımızı okuyabil-mek, kalıtımsal hastalıklarımızın altında yatan nedenleri anlamak ve tedavi edebilmek için yeterli değildi. Genom modifikasyon teknikle-rinin geliştirilmesi için on yıla yakın bir zaman beklememiz gerekti.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7e166a61e4ac68864231fa96db3475b9" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":52308442,"asset_id":32046939,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/52308442/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="32046939"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="32046939"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 32046939; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=32046939]").text(description); $(".js-view-count[data-work-id=32046939]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 32046939; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='32046939']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 32046939, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7e166a61e4ac68864231fa96db3475b9" } } $('.js-work-strip[data-work-id=32046939]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":32046939,"title":"Biyoendüstri 2.0: CRISPR Genom Modifikasyonu","translated_title":"","metadata":{"abstract":"18. yüzyılı takiben artan bilimsel gelişmeler ve buhar gücüyle çalışan makinelerin artması, dünya-mızda sanayi devrimi olarak adlandırdığımız yeni bir çağın başlamasına sebep oldu. Makinelerin gelişmesi ve bilgisayarın hayatımızın her anına daha etkin nü-fuz etmesiyle artık Endüstri 4.0'ı yaşamaya başladık. Biyoloji ve genetik alanında, her ne kadar aynı zaman dilimi içerisinde bilimsel çalışmalar yapılsa da Biyoendüstri diye adlandırabileceğimiz bir çağın ikinci versiyonuna geçmek için 2000'li yılları beklemek zorunda kaldık. Zira canlıları daha iyi anlayabilmek ve onlar-dan beklentilerimizi de yönlendirebilmek için mikro dünyaya inebilmemiz ve etki etme potansiyelimizi arttırabilmemiz gerekmekteydi. 2001'de üç milyar harften oluşan insan genomu tamamıyla deşifre edildiğinde çok az insan bunun aslında başlangıç olduğunu öngörüyordu. Çünkü DNA kitabımızı okuyabil-mek, kalıtımsal hastalıklarımızın altında yatan nedenleri anlamak ve tedavi edebilmek için yeterli değildi. Genom modifikasyon teknikle-rinin geliştirilmesi için on yıla yakın bir zaman beklememiz gerekti.","publication_name":"Popular Science-Türkiye"},"translated_abstract":"18. yüzyılı takiben artan bilimsel gelişmeler ve buhar gücüyle çalışan makinelerin artması, dünya-mızda sanayi devrimi olarak adlandırdığımız yeni bir çağın başlamasına sebep oldu. Makinelerin gelişmesi ve bilgisayarın hayatımızın her anına daha etkin nü-fuz etmesiyle artık Endüstri 4.0'ı yaşamaya başladık. Biyoloji ve genetik alanında, her ne kadar aynı zaman dilimi içerisinde bilimsel çalışmalar yapılsa da Biyoendüstri diye adlandırabileceğimiz bir çağın ikinci versiyonuna geçmek için 2000'li yılları beklemek zorunda kaldık. Zira canlıları daha iyi anlayabilmek ve onlar-dan beklentilerimizi de yönlendirebilmek için mikro dünyaya inebilmemiz ve etki etme potansiyelimizi arttırabilmemiz gerekmekteydi. 2001'de üç milyar harften oluşan insan genomu tamamıyla deşifre edildiğinde çok az insan bunun aslında başlangıç olduğunu öngörüyordu. Çünkü DNA kitabımızı okuyabil-mek, kalıtımsal hastalıklarımızın altında yatan nedenleri anlamak ve tedavi edebilmek için yeterli değildi. Genom modifikasyon teknikle-rinin geliştirilmesi için on yıla yakın bir zaman beklememiz gerekti.","internal_url":"https://www.academia.edu/32046939/Biyoendu_stri_2_0_CRISPR_Genom_Modifikasyonu","translated_internal_url":"","created_at":"2017-03-26T00:16:37.280-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":52308442,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/52308442/thumbnails/1.jpg","file_name":"Biyoendustri.pdf","download_url":"https://www.academia.edu/attachments/52308442/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Biyoendu_stri_2_0_CRISPR_Genom_Modifikas.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/52308442/Biyoendustri-libre.pdf?1490513808=\u0026response-content-disposition=attachment%3B+filename%3DBiyoendu_stri_2_0_CRISPR_Genom_Modifikas.pdf\u0026Expires=1732739029\u0026Signature=YOM4mgUAMduu3w5lPsGYAShSrDfZbiV1ETf5yL3EdNhi9Cnfr1Xinrbv5vltd3x8xTH2UC0jvI1utdVQaecj-IPuOHfyhrGKkbQHwtCfUeSOB8OIFsizfslYQB2rhP2n8dibccc8oXKe~hV2Klo2s1VlHA6mwfu~HQQR~s~DypCwHr4zpEuc13tnRBax7uyCOYwE47Hcs4oqmy-h16uulY-CLniixgkXloDh9GaBmr23dhTYT9T26BcvOixH8aIRAu7dmROq0jnAdlZPgI~KCcYARmKUjsMGSQ~lGwQlGXuFwRnDR0R1FfJorwMZmxqVMV84YB88FPKOj4dl2nXTuw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Biyoendu_stri_2_0_CRISPR_Genom_Modifikasyonu","translated_slug":"","page_count":3,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":52308442,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/52308442/thumbnails/1.jpg","file_name":"Biyoendustri.pdf","download_url":"https://www.academia.edu/attachments/52308442/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Biyoendu_stri_2_0_CRISPR_Genom_Modifikas.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/52308442/Biyoendustri-libre.pdf?1490513808=\u0026response-content-disposition=attachment%3B+filename%3DBiyoendu_stri_2_0_CRISPR_Genom_Modifikas.pdf\u0026Expires=1732739029\u0026Signature=YOM4mgUAMduu3w5lPsGYAShSrDfZbiV1ETf5yL3EdNhi9Cnfr1Xinrbv5vltd3x8xTH2UC0jvI1utdVQaecj-IPuOHfyhrGKkbQHwtCfUeSOB8OIFsizfslYQB2rhP2n8dibccc8oXKe~hV2Klo2s1VlHA6mwfu~HQQR~s~DypCwHr4zpEuc13tnRBax7uyCOYwE47Hcs4oqmy-h16uulY-CLniixgkXloDh9GaBmr23dhTYT9T26BcvOixH8aIRAu7dmROq0jnAdlZPgI~KCcYARmKUjsMGSQ~lGwQlGXuFwRnDR0R1FfJorwMZmxqVMV84YB88FPKOj4dl2nXTuw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1916835,"name":"CRISPR-Cas9 system","url":"https://www.academia.edu/Documents/in/CRISPR-Cas9_system"}],"urls":[]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="25658109"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/25658109/Ki%C5%9Fiye_%C3%96zel_Modern_Kanser_Tedavisi_Geneti%C4%9Fi_De%C4%9Fi%C5%9Ftirilmi%C5%9F_CAR_T_H%C3%BCcre_Terapisi"><img alt="Research paper thumbnail of Kişiye Özel Modern Kanser Tedavisi: Genetiği Değiştirilmiş CAR-T Hücre Terapisi" class="work-thumbnail" src="https://attachments.academia-assets.com/45995199/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/25658109/Ki%C5%9Fiye_%C3%96zel_Modern_Kanser_Tedavisi_Geneti%C4%9Fi_De%C4%9Fi%C5%9Ftirilmi%C5%9F_CAR_T_H%C3%BCcre_Terapisi">Kişiye Özel Modern Kanser Tedavisi: Genetiği Değiştirilmiş CAR-T Hücre Terapisi</a></div><div class="wp-workCard_item"><span>Biomedya</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Moleküler biyoloji ve genetik modifikasyon teknoloji ve olanaklarının gelişmesi ve ucuzlaması yıl...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Moleküler biyoloji ve genetik modifikasyon teknoloji ve olanaklarının gelişmesi ve ucuzlaması yıllardır kemoterapiye sınırlı olan kanser tedavilerinde bir çağın kapanıp yeni bir çağın açılmasına sebep oldu. Kemoterapide kullanılan ilaçlar ile sadece kanserli hücrelerin değil; vücuttaki sağlıklı hücrelerinde ölmesinden dolayı bilim insanları kanserli hastaya özel kişisel terapi teknikleri geliştirmek üzere yoğun çaba gösteriyorlar. Bu girişimlerden en umut verici sonuçlar alınan biri de ‘Genetiği Değiştirilmiş CAR-T Hücre Terapisi’...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="121a09fc43c118e905050400584fbe58" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45995199,"asset_id":25658109,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45995199/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="25658109"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="25658109"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 25658109; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=25658109]").text(description); $(".js-view-count[data-work-id=25658109]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 25658109; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='25658109']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 25658109, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "121a09fc43c118e905050400584fbe58" } } $('.js-work-strip[data-work-id=25658109]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":25658109,"title":"Kişiye Özel Modern Kanser Tedavisi: Genetiği Değiştirilmiş CAR-T Hücre Terapisi","translated_title":"","metadata":{"abstract":"Moleküler biyoloji ve genetik modifikasyon teknoloji ve olanaklarının gelişmesi ve ucuzlaması yıllardır kemoterapiye sınırlı olan kanser tedavilerinde bir çağın kapanıp yeni bir çağın açılmasına sebep oldu. Kemoterapide kullanılan ilaçlar ile sadece kanserli hücrelerin değil; vücuttaki sağlıklı hücrelerinde ölmesinden dolayı bilim insanları kanserli hastaya özel kişisel terapi teknikleri geliştirmek üzere yoğun çaba gösteriyorlar. Bu girişimlerden en umut verici sonuçlar alınan biri de ‘Genetiği Değiştirilmiş CAR-T Hücre Terapisi’...","publication_name":"Biomedya"},"translated_abstract":"Moleküler biyoloji ve genetik modifikasyon teknoloji ve olanaklarının gelişmesi ve ucuzlaması yıllardır kemoterapiye sınırlı olan kanser tedavilerinde bir çağın kapanıp yeni bir çağın açılmasına sebep oldu. Kemoterapide kullanılan ilaçlar ile sadece kanserli hücrelerin değil; vücuttaki sağlıklı hücrelerinde ölmesinden dolayı bilim insanları kanserli hastaya özel kişisel terapi teknikleri geliştirmek üzere yoğun çaba gösteriyorlar. 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İnsan genetik materyali on yıllar boyunca bilim insanlarının ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">İnsan Genomu Okuma OUT; Oynama IN!<br /><br />İnsan genetik materyali on yıllar boyunca bilim insanlarının sayısız sorularının kaynağı oldu. Ancak biyologların aradıkları cevapları bulabilmeleri için moleküler biyoloji teknolojilerinin gelişmesini beklemekten başka çareleri yoktu. Simdi DNA kitabımızı okuyabiliyoruz; ancak bu yetenek, kalıtımsal hastalıklarımızı anlamak ve tedavi edebilmek için yeterli değil. Geliştirilen birçok genom modifikasyon tekniklerine (örneğin; Zinc-finger ve TALEN (Transcription Activator-Like Effector Nuclase) nükleazları) rağmen, hiçbiri 2013'de yaygınlık kazanan CRISPR tekniğinin sağladığı hız, kolaylık ve fiyat ucuzluğu ile boy ölçüşemedi.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c27576562a5605e0da24ac55a41803c7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":47132922,"asset_id":22956016,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/47132922/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="22956016"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="22956016"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 22956016; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=22956016]").text(description); $(".js-view-count[data-work-id=22956016]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 22956016; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='22956016']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 22956016, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c27576562a5605e0da24ac55a41803c7" } } $('.js-work-strip[data-work-id=22956016]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":22956016,"title":"CRISPR Devrimi","translated_title":"","metadata":{"abstract":"İnsan Genomu Okuma OUT; Oynama IN!\n\nİnsan genetik materyali on yıllar boyunca bilim insanlarının sayısız sorularının kaynağı oldu. Ancak biyologların aradıkları cevapları bulabilmeleri için moleküler biyoloji teknolojilerinin gelişmesini beklemekten başka çareleri yoktu. Simdi DNA kitabımızı okuyabiliyoruz; ancak bu yetenek, kalıtımsal hastalıklarımızı anlamak ve tedavi edebilmek için yeterli değil. Geliştirilen birçok genom modifikasyon tekniklerine (örneğin; Zinc-finger ve TALEN (Transcription Activator-Like Effector Nuclase) nükleazları) rağmen, hiçbiri 2013'de yaygınlık kazanan CRISPR tekniğinin sağladığı hız, kolaylık ve fiyat ucuzluğu ile boy ölçüşemedi.","publication_name":"Biomedya"},"translated_abstract":"İnsan Genomu Okuma OUT; Oynama IN!\n\nİnsan genetik materyali on yıllar boyunca bilim insanlarının sayısız sorularının kaynağı oldu. Ancak biyologların aradıkları cevapları bulabilmeleri için moleküler biyoloji teknolojilerinin gelişmesini beklemekten başka çareleri yoktu. Simdi DNA kitabımızı okuyabiliyoruz; ancak bu yetenek, kalıtımsal hastalıklarımızı anlamak ve tedavi edebilmek için yeterli değil. 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Bu hasta...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Dünya genelinde yaklaşık 7000’den fazla nadir hastalık çeşitliliği olduğu bilinmektedir. Bu hastalıkların özellikle ülkemizde görülme oranı diğer ülkelere göre oldukça fazladır. Bunun en büyük sebebi olarak yaygın akraba evlilikleri gösterilebilir. Genetik sorunlar sonucu oluşan bu hastalıkların birçoğunun tedavisi günümüzde bulunmamaktadır. Olan tedaviler ise oldukça pahalıdır. Bu ve bunun gibi sorunlar görüldüğünde nadir hastalık sahibi olan bireylere bir çıkış yolu aramak ve en nihayetinde bir tedavi bulabilmek için farklı çalışmalara öncülük edebilmek amacı ile RaDiChal kurucu direktörümüz Dr. Cihan Taştan (Acıbadem Labcell Laboratuvarı Ar&Ge Sorumlusu) öncülüğünde lisans düzeyindeki öğrenciler ile RaDiChal ekibi oluşturuldu. Bu ekip doğrultusunda başlatılan yarışmamız nadir hastalıklar ve tedavileri konulu Türkiye genelinde genetik araştırmaların yapılması hedeflenen bir yarışmadır.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7fff2823240f709afd9e028fef87763e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":62218047,"asset_id":42088364,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/62218047/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="42088364"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="42088364"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42088364; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42088364]").text(description); $(".js-view-count[data-work-id=42088364]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 42088364; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='42088364']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 42088364, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7fff2823240f709afd9e028fef87763e" } } $('.js-work-strip[data-work-id=42088364]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":42088364,"title":"RARE DISEASES CHALLANGE 2020 Tanıtım Dosyası","translated_title":"","metadata":{"abstract":"Dünya genelinde yaklaşık 7000’den fazla nadir hastalık çeşitliliği olduğu bilinmektedir. Bu hastalıkların özellikle ülkemizde görülme oranı diğer ülkelere göre oldukça fazladır. Bunun en büyük sebebi olarak yaygın akraba evlilikleri gösterilebilir. Genetik sorunlar sonucu oluşan bu hastalıkların birçoğunun tedavisi günümüzde bulunmamaktadır. Olan tedaviler ise oldukça pahalıdır. Bu ve bunun gibi sorunlar görüldüğünde nadir hastalık sahibi olan bireylere bir çıkış yolu aramak ve en nihayetinde bir tedavi bulabilmek için farklı çalışmalara öncülük edebilmek amacı ile RaDiChal kurucu direktörümüz Dr. Cihan Taştan (Acıbadem Labcell Laboratuvarı Ar\u0026Ge Sorumlusu) öncülüğünde lisans düzeyindeki öğrenciler ile RaDiChal ekibi oluşturuldu. Bu ekip doğrultusunda başlatılan yarışmamız nadir hastalıklar ve tedavileri konulu Türkiye genelinde genetik araştırmaların yapılması hedeflenen bir yarışmadır."},"translated_abstract":"Dünya genelinde yaklaşık 7000’den fazla nadir hastalık çeşitliliği olduğu bilinmektedir. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="37203315"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/37203315/CRISPR_mediated_knock_out_of_Immune_Checkpoint_Inhibitors_for_Cancer_Therapy_in_Turkey"><img alt="Research paper thumbnail of CRISPR mediated knock-out of Immune Checkpoint Inhibitors for Cancer Therapy in Turkey" class="work-thumbnail" src="https://attachments.academia-assets.com/57154114/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37203315/CRISPR_mediated_knock_out_of_Immune_Checkpoint_Inhibitors_for_Cancer_Therapy_in_Turkey">CRISPR mediated knock-out of Immune Checkpoint Inhibitors for Cancer Therapy in Turkey</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/OsmanDoluca">Osman Doluca</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/kaany%C4%B1lanc%C4%B1o%C4%9Flu">kaan yılancıoğlu</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cancer is a group of diseases characterized by abnormal cell growth, and is the first leading cau...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cancer is a group of diseases characterized by abnormal cell growth, and is the first leading cause of human deaths in underdeveloped countries and the second in the developed world. According to 2015 statistics from the World Health Organization, approximately 9 million people die from cancer every year, and millions of new cancer cases are being diagnosed annually, revealing the frightening dimensions of the situation. Cancer treatment costs billions of dollars each year, aiming to improve the quality of cancer patient's life. The current available treatments are not enough to cure the most types of this disease and effective in only a fraction of the cancer cases.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="483ef58aaa2858ece605c96f412e8d96" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":57154114,"asset_id":37203315,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/57154114/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37203315"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37203315"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37203315; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37203315]").text(description); $(".js-view-count[data-work-id=37203315]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37203315; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37203315']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37203315, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "483ef58aaa2858ece605c96f412e8d96" } } $('.js-work-strip[data-work-id=37203315]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37203315,"title":"CRISPR mediated knock-out of Immune Checkpoint Inhibitors for Cancer Therapy in Turkey","translated_title":"","metadata":{"abstract":"Cancer is a group of diseases characterized by abnormal cell growth, and is the first leading cause of human deaths in underdeveloped countries and the second in the developed world. 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At the beginning of the 2000s, upon the introduction of the human genome project, genetic disorders that have caused many diseases have come to light and the development of gene therapy methods has been accelerated to repair these damaged DNA sequences.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="103c309c76d12aacf76eafc4a97f58e6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":60179518,"asset_id":39987607,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/60179518/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="39987607"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="39987607"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 39987607; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=39987607]").text(description); $(".js-view-count[data-work-id=39987607]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 39987607; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='39987607']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 39987607, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "103c309c76d12aacf76eafc4a97f58e6" } } $('.js-work-strip[data-work-id=39987607]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":39987607,"title":"The Future of Medicine with Genetic Engineering Techniques: Gene Therapies","translated_title":"","metadata":{"abstract":"Gene therapy is a technique that modifies a part of a person's genome, suffering from hereditary/familial/rare diseases, to repair a defective gene to maintain its functional phenotype. 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Ulusal Kan Merkezleri ve Transfüzyon Tıbbı Kongresi</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Genetik tedavi (gen terapisi), kalıtsal/ailesel/nadir hastalıklardan muzdarip bir kişinin genomun...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Genetik tedavi (gen terapisi), kalıtsal/ailesel/nadir hastalıklardan muzdarip bir kişinin genomundaki bozuk bir geni<br />tamir etmek veya fonksiyonel özelliğini devam ettirmek için kişinin genetiğinin bir bölümünü değiştiren bir tekniktir.<br />2000’li yılların başında sağlıklı insan genom şifresinin ortaya konulmasıyla birçok hastalığın altında yatan genetik bozukluklar<br />gün yüzüne çıkmış ve bu hasarlı DNA dizilerini tamir etmek üzere gen terapi yöntemlerinin geliştirilmesi hız<br />kazanmıştır.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d079c52292e4a44be42847289fec6988" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":59311265,"asset_id":39184331,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/59311265/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="39184331"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="39184331"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 39184331; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=39184331]").text(description); $(".js-view-count[data-work-id=39184331]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 39184331; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='39184331']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 39184331, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d079c52292e4a44be42847289fec6988" } } $('.js-work-strip[data-work-id=39184331]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":39184331,"title":"Gen Mühendisliği Ürünleri ile Genetik Terapi","translated_title":"","metadata":{"abstract":"Genetik tedavi (gen terapisi), kalıtsal/ailesel/nadir hastalıklardan muzdarip bir kişinin genomundaki bozuk bir geni\ntamir etmek veya fonksiyonel özelliğini devam ettirmek için kişinin genetiğinin bir bölümünü değiştiren bir tekniktir.\n2000’li yılların başında sağlıklı insan genom şifresinin ortaya konulmasıyla birçok hastalığın altında yatan genetik bozukluklar\ngün yüzüne çıkmış ve bu hasarlı DNA dizilerini tamir etmek üzere gen terapi yöntemlerinin geliştirilmesi hız\nkazanmıştır.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"XII. 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Nasıl Çalışır?" class="work-thumbnail" src="https://attachments.academia-assets.com/59014656/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/38914874/Genetik_Tedaviler_Nelerdir_Nas%C4%B1l_%C3%87al%C4%B1%C5%9F%C4%B1r">Genetik Tedaviler Nelerdir? Nasıl Çalışır?</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Gen tedavisi, genetik hastalıklardan muzdarip bir kişinin genomundaki bozuk bir geni tamir etmek ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Gen tedavisi, genetik hastalıklardan muzdarip bir kişinin genomundaki bozuk bir geni tamir etmek veya<br />fonksiyonel özelliğini sağlamak için kişinin genetiğinin bir bölümünü değiştiren bir tekniktir. 2000’li<br />yılların başında sağlıklı insan genom şifresinin ortaya konulmasıyla birçok hastalığın altında yatan<br />genetik bozukluklar gün yüzüne çıkmış ve bu hasarlı DNA dizilerini tamir etmek veya işlevsel bir<br />formuyla telafi etmek üzere gen tedavi yöntemlerinin geliştirilmesi hız kazanmıştır.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0469594cfa8d9d429bbf6f10bf811782" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":59014656,"asset_id":38914874,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/59014656/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="38914874"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="38914874"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 38914874; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=38914874]").text(description); $(".js-view-count[data-work-id=38914874]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 38914874; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='38914874']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 38914874, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0469594cfa8d9d429bbf6f10bf811782" } } $('.js-work-strip[data-work-id=38914874]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":38914874,"title":"Genetik Tedaviler Nelerdir? Nasıl Çalışır?","translated_title":"","metadata":{"abstract":"Gen tedavisi, genetik hastalıklardan muzdarip bir kişinin genomundaki bozuk bir geni tamir etmek veya\nfonksiyonel özelliğini sağlamak için kişinin genetiğinin bir bölümünü değiştiren bir tekniktir. 2000’li\nyılların başında sağlıklı insan genom şifresinin ortaya konulmasıyla birçok hastalığın altında yatan\ngenetik bozukluklar gün yüzüne çıkmış ve bu hasarlı DNA dizilerini tamir etmek veya işlevsel bir\nformuyla telafi etmek üzere gen tedavi yöntemlerinin geliştirilmesi hız kazanmıştır.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}}},"translated_abstract":"Gen tedavisi, genetik hastalıklardan muzdarip bir kişinin genomundaki bozuk bir geni tamir etmek veya\nfonksiyonel özelliğini sağlamak için kişinin genetiğinin bir bölümünü değiştiren bir tekniktir. 2000’li\nyılların başında sağlıklı insan genom şifresinin ortaya konulmasıyla birçok hastalığın altında yatan\ngenetik bozukluklar gün yüzüne çıkmış ve bu hasarlı DNA dizilerini tamir etmek veya işlevsel bir\nformuyla telafi etmek üzere gen tedavi yöntemlerinin geliştirilmesi hız kazanmıştır.","internal_url":"https://www.academia.edu/38914874/Genetik_Tedaviler_Nelerdir_Nas%C4%B1l_%C3%87al%C4%B1%C5%9F%C4%B1r","translated_internal_url":"","created_at":"2019-04-24T03:55:38.903-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[],"downloadable_attachments":[{"id":59014656,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/59014656/thumbnails/1.jpg","file_name":"Gen_tedavileri20190424-101382-13v778b.pdf","download_url":"https://www.academia.edu/attachments/59014656/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genetik_Tedaviler_Nelerdir_Nasil_Calisir.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/59014656/Gen_tedavileri20190424-101382-13v778b-libre.pdf?1556116110=\u0026response-content-disposition=attachment%3B+filename%3DGenetik_Tedaviler_Nelerdir_Nasil_Calisir.pdf\u0026Expires=1732739029\u0026Signature=Iu-9ItSKtHIlMb5Qew1QkPWu3o3bmXidHhp6EPoSgGZzOLntAoKgneQb3frKK3VsiEfVFld39HqWgvj3TB~AiuBY8nkvAcgQVG5f0YPdKAnPiOfqMx7gvdYxWz85VpS1ZN~PHgvvfMLaiE5enukaI6h574T2oGRuvxyuhOtB~tJp57OA7bQ7T1y3Xea5vzkuy1A5vQE~5iXroIt8~QawkQ0qHpQSqh5~8IDq-tbityrigbvPCI2aTdwIHL-jmE1oLZ2U-tRh-i~-jurn~QfqErtgT-SdwWZPb0o7ick-cCnfup5zon~5h31QczSsbTSqrxXH5AHJkBzQDyZXnjMkPQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Genetik_Tedaviler_Nelerdir_Nasıl_Çalışır","translated_slug":"","page_count":10,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":59014656,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/59014656/thumbnails/1.jpg","file_name":"Gen_tedavileri20190424-101382-13v778b.pdf","download_url":"https://www.academia.edu/attachments/59014656/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genetik_Tedaviler_Nelerdir_Nasil_Calisir.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/59014656/Gen_tedavileri20190424-101382-13v778b-libre.pdf?1556116110=\u0026response-content-disposition=attachment%3B+filename%3DGenetik_Tedaviler_Nelerdir_Nasil_Calisir.pdf\u0026Expires=1732739029\u0026Signature=Iu-9ItSKtHIlMb5Qew1QkPWu3o3bmXidHhp6EPoSgGZzOLntAoKgneQb3frKK3VsiEfVFld39HqWgvj3TB~AiuBY8nkvAcgQVG5f0YPdKAnPiOfqMx7gvdYxWz85VpS1ZN~PHgvvfMLaiE5enukaI6h574T2oGRuvxyuhOtB~tJp57OA7bQ7T1y3Xea5vzkuy1A5vQE~5iXroIt8~QawkQ0qHpQSqh5~8IDq-tbityrigbvPCI2aTdwIHL-jmE1oLZ2U-tRh-i~-jurn~QfqErtgT-SdwWZPb0o7ick-cCnfup5zon~5h31QczSsbTSqrxXH5AHJkBzQDyZXnjMkPQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="38914863"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/38914863/CD19_Spesifik_CAR_T_H%C3%BCcreler_ISIKOK_19_Ac%C4%B1badem_Labcell_Preklinik_in_vivo_%C3%87al%C4%B1%C5%9Fma_Verileri"><img alt="Research paper thumbnail of CD19 Spesifik CAR T Hücreler (ISIKOK 19) – Acıbadem Labcell Preklinik in vivo Çalışma Verileri" class="work-thumbnail" src="https://attachments.academia-assets.com/59014645/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/38914863/CD19_Spesifik_CAR_T_H%C3%BCcreler_ISIKOK_19_Ac%C4%B1badem_Labcell_Preklinik_in_vivo_%C3%87al%C4%B1%C5%9Fma_Verileri">CD19 Spesifik CAR T Hücreler (ISIKOK 19) – Acıbadem Labcell Preklinik in vivo Çalışma Verileri</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/DeryaDilekKanca%C4%9F%C4%B1">Derya Dilek Kancağı</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://yeditepe.academia.edu/RaifeDilekTURAN">Raife Dilek TURAN</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Moleküler biyoloji ve genetik modifikasyon olanaklarının gelişmesi ve ucuzlaması yıllardır kemote...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Moleküler biyoloji ve genetik modifikasyon olanaklarının gelişmesi ve ucuzlaması yıllardır<br />kemoterapi ile sınırlı olan kanser tedavilerinde bir çağın kapanıp yeni bir çağın açılmasına sebep<br />olmuştur. Kemoterapide kullanılan ilaçlar ile sadece kanserli hücrelerin değil; vücuttaki sağlıklı<br />hücrelerin de zarar görmesinden dolayı kanserli hastaya özel kişisel tedavi teknikleri geliştirmek<br />üzere yoğun çaba sarf edilmektedir. Bu girişimlerden en umut verici sonuçlardan biri genetiği<br />değiştirilmiş CAR-T Hücre tedavisidir. Bu amaçla çalışmamızda lentiviral vektörler kullanılarak<br />FMC63-CD8-CD28-CD3z-EGFRt sekansına sahip CD19 antijenine spesifik antikor başlığı içeren<br />kimerik antijen reseptör eksprese eden T hücreler üretilmiş ve in vitro analizleri tamamlanarak NOD<br />SCID fare gruplarında preklinik in vivo çalışmalar başarıyla tamamlanmıştır (ACU-DEHAM).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d73fc3246fdc459e81464af57600f42c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":59014645,"asset_id":38914863,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/59014645/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="38914863"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="38914863"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 38914863; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=38914863]").text(description); $(".js-view-count[data-work-id=38914863]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 38914863; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='38914863']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 38914863, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d73fc3246fdc459e81464af57600f42c" } } $('.js-work-strip[data-work-id=38914863]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":38914863,"title":"CD19 Spesifik CAR T Hücreler (ISIKOK 19) – Acıbadem Labcell Preklinik in vivo Çalışma Verileri","translated_title":"","metadata":{"abstract":"Moleküler biyoloji ve genetik modifikasyon olanaklarının gelişmesi ve ucuzlaması yıllardır\nkemoterapi ile sınırlı olan kanser tedavilerinde bir çağın kapanıp yeni bir çağın açılmasına sebep\nolmuştur. Kemoterapide kullanılan ilaçlar ile sadece kanserli hücrelerin değil; vücuttaki sağlıklı\nhücrelerin de zarar görmesinden dolayı kanserli hastaya özel kişisel tedavi teknikleri geliştirmek\nüzere yoğun çaba sarf edilmektedir. Bu girişimlerden en umut verici sonuçlardan biri genetiği\ndeğiştirilmiş CAR-T Hücre tedavisidir. Bu amaçla çalışmamızda lentiviral vektörler kullanılarak\nFMC63-CD8-CD28-CD3z-EGFRt sekansına sahip CD19 antijenine spesifik antikor başlığı içeren\nkimerik antijen reseptör eksprese eden T hücreler üretilmiş ve in vitro analizleri tamamlanarak NOD\nSCID fare gruplarında preklinik in vivo çalışmalar başarıyla tamamlanmıştır (ACU-DEHAM).","publication_date":{"day":null,"month":null,"year":2019,"errors":{}}},"translated_abstract":"Moleküler biyoloji ve genetik modifikasyon olanaklarının gelişmesi ve ucuzlaması yıllardır\nkemoterapi ile sınırlı olan kanser tedavilerinde bir çağın kapanıp yeni bir çağın açılmasına sebep\nolmuştur. Kemoterapide kullanılan ilaçlar ile sadece kanserli hücrelerin değil; vücuttaki sağlıklı\nhücrelerin de zarar görmesinden dolayı kanserli hastaya özel kişisel tedavi teknikleri geliştirmek\nüzere yoğun çaba sarf edilmektedir. Bu girişimlerden en umut verici sonuçlardan biri genetiği\ndeğiştirilmiş CAR-T Hücre tedavisidir. Bu amaçla çalışmamızda lentiviral vektörler kullanılarak\nFMC63-CD8-CD28-CD3z-EGFRt sekansına sahip CD19 antijenine spesifik antikor başlığı içeren\nkimerik antijen reseptör eksprese eden T hücreler üretilmiş ve in vitro analizleri tamamlanarak NOD\nSCID fare gruplarında preklinik in vivo çalışmalar başarıyla tamamlanmıştır (ACU-DEHAM).","internal_url":"https://www.academia.edu/38914863/CD19_Spesifik_CAR_T_H%C3%BCcreler_ISIKOK_19_Ac%C4%B1badem_Labcell_Preklinik_in_vivo_%C3%87al%C4%B1%C5%9Fma_Verileri","translated_internal_url":"","created_at":"2019-04-24T03:54:19.321-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[{"id":32483054,"work_id":38914863,"tagging_user_id":20976874,"tagged_user_id":80459237,"co_author_invite_id":null,"email":"d***i@acibademlabcell.com.tr","display_order":-1,"name":"Derya Dilek Kancağı","title":"CD19 Spesifik CAR T Hücreler (ISIKOK 19) – Acıbadem Labcell Preklinik in vivo Çalışma Verileri"},{"id":32483055,"work_id":38914863,"tagging_user_id":20976874,"tagged_user_id":67013666,"co_author_invite_id":null,"email":"d***1@gmail.com","affiliation":"Yeditepe University","display_order":1,"name":"Raife Dilek TURAN","title":"CD19 Spesifik CAR T Hücreler (ISIKOK 19) – Acıbadem Labcell Preklinik in vivo Çalışma Verileri"},{"id":32483056,"work_id":38914863,"tagging_user_id":20976874,"tagged_user_id":49124051,"co_author_invite_id":null,"email":"e***i@superonline.com","display_order":2,"name":"Ercument Ovali","title":"CD19 Spesifik CAR T Hücreler (ISIKOK 19) – Acıbadem Labcell Preklinik in vivo Çalışma Verileri"}],"downloadable_attachments":[{"id":59014645,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/59014645/thumbnails/1.jpg","file_name":"CAR-T_in_vivo_bildiri20190424-14980-v0qdqx.pdf","download_url":"https://www.academia.edu/attachments/59014645/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CD19_Spesifik_CAR_T_Hucreler_ISIKOK_19_A.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/59014645/CAR-T_in_vivo_bildiri20190424-14980-v0qdqx-libre.pdf?1556131527=\u0026response-content-disposition=attachment%3B+filename%3DCD19_Spesifik_CAR_T_Hucreler_ISIKOK_19_A.pdf\u0026Expires=1732704107\u0026Signature=H7hmPHm98TLMcEQysm-~AY1P4Kl5WL553UP7N83cRnbkAum3squbjEeZXjhc4v2bUi9l1AX-mCzGyh3zE~Ck8t6Mt8koMLOfHskgj6V~8LtMIkQRpyAKuaJ6dcCxuZaX5Y8XkIU5upPEy59j~QtwxHGxP6T3r9lpCC~V~NeiFy9hFG3MCnzfI9XuV5VHWoxoABgIOZZJIBfRrmcRDj6WbkuTf05O3gv8ae5l8CXHfUJLdtB92MXCHw0fA2WRzTG1ZAy15QNnrGh8N4hL2J6jkc3Q40QK-3ZeSNLYHIplnDRFjQ6xfivKDU0d4PcebFCP9EEiQVmsegI87E8YVwWtnQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"CD19_Spesifik_CAR_T_Hücreler_ISIKOK_19_Acıbadem_Labcell_Preklinik_in_vivo_Çalışma_Verileri","translated_slug":"","page_count":7,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"Nm8vZHc2OW1wZ292T3l0TkhzN1RoTjlYaEdaZTEwZ1BUYjVDRVZGNUoxM1FPUTEySTFYRGlJTSt5Nm8xRkJBZy0tWEZ4VmFKaFN4bHZldzdkT0VzSjFUdz09--87b63bc8d4a8c6020256a4845f1891718ff62256"},"attachments":[{"id":59014645,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/59014645/thumbnails/1.jpg","file_name":"CAR-T_in_vivo_bildiri20190424-14980-v0qdqx.pdf","download_url":"https://www.academia.edu/attachments/59014645/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CD19_Spesifik_CAR_T_Hucreler_ISIKOK_19_A.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/59014645/CAR-T_in_vivo_bildiri20190424-14980-v0qdqx-libre.pdf?1556131527=\u0026response-content-disposition=attachment%3B+filename%3DCD19_Spesifik_CAR_T_Hucreler_ISIKOK_19_A.pdf\u0026Expires=1732704107\u0026Signature=H7hmPHm98TLMcEQysm-~AY1P4Kl5WL553UP7N83cRnbkAum3squbjEeZXjhc4v2bUi9l1AX-mCzGyh3zE~Ck8t6Mt8koMLOfHskgj6V~8LtMIkQRpyAKuaJ6dcCxuZaX5Y8XkIU5upPEy59j~QtwxHGxP6T3r9lpCC~V~NeiFy9hFG3MCnzfI9XuV5VHWoxoABgIOZZJIBfRrmcRDj6WbkuTf05O3gv8ae5l8CXHfUJLdtB92MXCHw0fA2WRzTG1ZAy15QNnrGh8N4hL2J6jkc3Q40QK-3ZeSNLYHIplnDRFjQ6xfivKDU0d4PcebFCP9EEiQVmsegI87E8YVwWtnQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":22103,"name":"Cancer Immunotherapy","url":"https://www.academia.edu/Documents/in/Cancer_Immunotherapy"},{"id":991844,"name":"Chimeric Antigen Receptor","url":"https://www.academia.edu/Documents/in/Chimeric_Antigen_Receptor"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="38914850"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/38914850/CD19_Pozitif_Kanser_T%C3%BCrlerine_Spesifik_Transgenik_CAR_T_H%C3%BCcre_ISIKOK_19_%C4%B0n_Vitro_Etkinlik_Sonu%C3%A7lar%C4%B1"><img alt="Research paper thumbnail of CD19 Pozitif Kanser Türlerine Spesifik Transgenik CAR-T Hücre (ISIKOK- 19©) İn Vitro Etkinlik Sonuçları" class="work-thumbnail" src="https://attachments.academia-assets.com/59014631/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/38914850/CD19_Pozitif_Kanser_T%C3%BCrlerine_Spesifik_Transgenik_CAR_T_H%C3%BCcre_ISIKOK_19_%C4%B0n_Vitro_Etkinlik_Sonu%C3%A7lar%C4%B1">CD19 Pozitif Kanser Türlerine Spesifik Transgenik CAR-T Hücre (ISIKOK- 19©) İn Vitro Etkinlik Sonuçları</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/DeryaDilekKanca%C4%9F%C4%B1">Derya Dilek Kancağı</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://yeditepe.academia.edu/RaifeDilekTURAN">Raife Dilek TURAN</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Hematolojik ALL, NHL ve MM kanserlerinde relaps oranı yüksek olmakla birlikte, mevcut tedaviler t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Hematolojik ALL, NHL ve MM kanserlerinde relaps oranı yüksek olmakla birlikte, mevcut<br />tedaviler tam remisyon oluşturmada küratif değildir. Temel nedeni, kanser dokusuna karşı mevcut<br />immün hücrelerinin düşük aktiviteli reseptörler tarafından yeterince uyarılamamış olmasıdır. Bu<br />kanser türlerinin bir bölümü CD19 yüzey proteini ürettiğinden teröpatik hedef olarak<br />kullanılabilmektedir. Bu çalışmada, insan T lenfositlerinden CD19 pozitif kanserleri hedef alabilen<br />yüksek afiniteli kimerik antijen reseptörler (CAR) üreten transgenik CAR-T hücrelerinin (ISIKOK-<br />19©) üretilmesi ve pre-klinik modelde yüksek etkinlik seviyesinde sitotoksik sonuçlar elde edilmesi<br />amaçlanmıştır.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="028b0064c52753c89d10dd46462a6487" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":59014631,"asset_id":38914850,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/59014631/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="38914850"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="38914850"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 38914850; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=38914850]").text(description); $(".js-view-count[data-work-id=38914850]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 38914850; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='38914850']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 38914850, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "028b0064c52753c89d10dd46462a6487" } } $('.js-work-strip[data-work-id=38914850]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":38914850,"title":"CD19 Pozitif Kanser Türlerine Spesifik Transgenik CAR-T Hücre (ISIKOK- 19©) İn Vitro Etkinlik Sonuçları","translated_title":"","metadata":{"abstract":"Hematolojik ALL, NHL ve MM kanserlerinde relaps oranı yüksek olmakla birlikte, mevcut\ntedaviler tam remisyon oluşturmada küratif değildir. Temel nedeni, kanser dokusuna karşı mevcut\nimmün hücrelerinin düşük aktiviteli reseptörler tarafından yeterince uyarılamamış olmasıdır. Bu\nkanser türlerinin bir bölümü CD19 yüzey proteini ürettiğinden teröpatik hedef olarak\nkullanılabilmektedir. Bu çalışmada, insan T lenfositlerinden CD19 pozitif kanserleri hedef alabilen\nyüksek afiniteli kimerik antijen reseptörler (CAR) üreten transgenik CAR-T hücrelerinin (ISIKOK-\n19©) üretilmesi ve pre-klinik modelde yüksek etkinlik seviyesinde sitotoksik sonuçlar elde edilmesi\namaçlanmıştır.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}}},"translated_abstract":"Hematolojik ALL, NHL ve MM kanserlerinde relaps oranı yüksek olmakla birlikte, mevcut\ntedaviler tam remisyon oluşturmada küratif değildir. Temel nedeni, kanser dokusuna karşı mevcut\nimmün hücrelerinin düşük aktiviteli reseptörler tarafından yeterince uyarılamamış olmasıdır. 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Bu çalışmada, insan T lenfositlerinden CD19 pozitif kanserleri hedef alabilen\nyüksek afiniteli kimerik antijen reseptörler (CAR) üreten transgenik CAR-T hücrelerinin (ISIKOK-\n19©) üretilmesi ve pre-klinik modelde yüksek etkinlik seviyesinde sitotoksik sonuçlar elde edilmesi\namaçlanmıştır.","internal_url":"https://www.academia.edu/38914850/CD19_Pozitif_Kanser_T%C3%BCrlerine_Spesifik_Transgenik_CAR_T_H%C3%BCcre_ISIKOK_19_%C4%B0n_Vitro_Etkinlik_Sonu%C3%A7lar%C4%B1","translated_internal_url":"","created_at":"2019-04-24T03:52:20.419-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[{"id":32483043,"work_id":38914850,"tagging_user_id":20976874,"tagged_user_id":80459237,"co_author_invite_id":null,"email":"d***i@acibademlabcell.com.tr","display_order":1,"name":"Derya Dilek Kancağı","title":"CD19 Pozitif Kanser Türlerine Spesifik Transgenik CAR-T Hücre (ISIKOK- 19©) İn Vitro Etkinlik Sonuçları"},{"id":32483044,"work_id":38914850,"tagging_user_id":20976874,"tagged_user_id":67013666,"co_author_invite_id":null,"email":"d***1@gmail.com","affiliation":"Yeditepe University","display_order":2,"name":"Raife Dilek TURAN","title":"CD19 Pozitif Kanser Türlerine Spesifik Transgenik CAR-T Hücre (ISIKOK- 19©) İn Vitro Etkinlik Sonuçları"},{"id":32483045,"work_id":38914850,"tagging_user_id":20976874,"tagged_user_id":49124051,"co_author_invite_id":null,"email":"e***i@superonline.com","display_order":3,"name":"Ercument Ovali","title":"CD19 Pozitif Kanser Türlerine Spesifik Transgenik CAR-T Hücre (ISIKOK- 19©) İn Vitro Etkinlik Sonuçları"}],"downloadable_attachments":[{"id":59014631,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/59014631/thumbnails/1.jpg","file_name":"CAR-T_in_vitro_bildiri20190424-15524-19mhkdr.pdf","download_url":"https://www.academia.edu/attachments/59014631/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CD19_Pozitif_Kanser_Turlerine_Spesifik_T.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/59014631/CAR-T_in_vitro_bildiri20190424-15524-19mhkdr-libre.pdf?1556116133=\u0026response-content-disposition=attachment%3B+filename%3DCD19_Pozitif_Kanser_Turlerine_Spesifik_T.pdf\u0026Expires=1732704107\u0026Signature=Ma91N6tGD9kYxUQ-7-fQHd0lrc173MYqKxxJY-RfG~AdfvTERFi4w-40CwC2D4aTYRncEjb-ek0iRZmbNKv2W0nlboe6aHqdMaXECWUmO6bwa-C1o5loMRYhytsONAEmkgFcGvwGig2GRW~a2Op0dfs5OqZJK9fGRH4CUHWhh~9sDik35TiJcb1aq~vE~0Un-pKaKs3LXhbsnAFhFQiFaBkT3iIetcEIQFIkKYVVxgCs9FqebvWqwaBAYAs9z4TKTVUVz-ABx9pgZeX-QxJ60iKfMmm4WiZEYqLvKjsXjcUC4SbNdW0vb0UoZKQkBYxDVBExmXo1yAN~gIp5D8vXcQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"CD19_Pozitif_Kanser_Türlerine_Spesifik_Transgenik_CAR_T_Hücre_ISIKOK_19_İn_Vitro_Etkinlik_Sonuçları","translated_slug":"","page_count":6,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"LzF4NndIaGRDU2p5N1o4ZzF6ZzdYQWYrOW0vTUhPcExNZHhyeENNNUF3Z0lmOGJKZkJ6T25YKysveTRWY0ZubC0tOFBmWUp3ZE82L1NmSkllaFArUTJOQT09--63f272022077bab945a2dbeb2a0c4e7abf5dd566"},"attachments":[{"id":59014631,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/59014631/thumbnails/1.jpg","file_name":"CAR-T_in_vitro_bildiri20190424-15524-19mhkdr.pdf","download_url":"https://www.academia.edu/attachments/59014631/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CD19_Pozitif_Kanser_Turlerine_Spesifik_T.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/59014631/CAR-T_in_vitro_bildiri20190424-15524-19mhkdr-libre.pdf?1556116133=\u0026response-content-disposition=attachment%3B+filename%3DCD19_Pozitif_Kanser_Turlerine_Spesifik_T.pdf\u0026Expires=1732704107\u0026Signature=Ma91N6tGD9kYxUQ-7-fQHd0lrc173MYqKxxJY-RfG~AdfvTERFi4w-40CwC2D4aTYRncEjb-ek0iRZmbNKv2W0nlboe6aHqdMaXECWUmO6bwa-C1o5loMRYhytsONAEmkgFcGvwGig2GRW~a2Op0dfs5OqZJK9fGRH4CUHWhh~9sDik35TiJcb1aq~vE~0Un-pKaKs3LXhbsnAFhFQiFaBkT3iIetcEIQFIkKYVVxgCs9FqebvWqwaBAYAs9z4TKTVUVz-ABx9pgZeX-QxJ60iKfMmm4WiZEYqLvKjsXjcUC4SbNdW0vb0UoZKQkBYxDVBExmXo1yAN~gIp5D8vXcQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":991844,"name":"Chimeric Antigen Receptor","url":"https://www.academia.edu/Documents/in/Chimeric_Antigen_Receptor"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="37562975"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/37562975/Hope_and_Cure_for_Treatment_of_Genetic_Rare_Diseases_with_CRISPR_Genome_Repair_Technology"><img alt="Research paper thumbnail of Hope and Cure for Treatment of Genetic / Rare Diseases with CRISPR Genome Repair Technology" class="work-thumbnail" src="https://attachments.academia-assets.com/57539840/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37562975/Hope_and_Cure_for_Treatment_of_Genetic_Rare_Diseases_with_CRISPR_Genome_Repair_Technology">Hope and Cure for Treatment of Genetic / Rare Diseases with CRISPR Genome Repair Technology</a></div><div class="wp-workCard_item"><span>The 5th International and Interdisciplinary Conference onHealth, Culture and the Human Body</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mostly genetic-based, approximately 7000 rare diseases have been identified globally. Most of the...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mostly genetic-based, approximately 7000 rare diseases have been identified globally. Most of the rare diseases affect the quality of life considerably. In this respect, studies on the development of trustable and efficient genetically-based treatment methods are very critical. New designed nuclease-targeted genomic regulation techniques are exciting for the biomedical research field and providing a hope for clinical applications as never before. The CRISPR/Cas system paved the way for the manipulation of gene expression or regulation and led to the questioning of gene functions in mammalian cell lines in vitro and in many different organisms in vivo including insects, plants, animals and now, humans. This genetic engineering approach is a highly efficient and scalable biotechnological tool that generates genome-wide mutations through sequence-specific target guide RNAs to induce double-stranded DNA breaks. These man-made mutations enable to fix or repair the site of disease-related genetic disorders with the help of homology-directed repair approach. Thousands of scientific publications have been recently published on CRISPR/Cas technology that demonstrates successfully repaired or altered DNA sequence in cells. Here, I will illustrate by examples how tens of genetically-based diseases are treated in the laboratory or in animal models using CRISPR/Cas genome repair technology, which pushes biotechnology and therapeutic companies to the genetic therapies.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8b7770aa69900327a7ce0d3b5b7f5688" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":57539840,"asset_id":37562975,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/57539840/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37562975"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37562975"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37562975; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37562975]").text(description); $(".js-view-count[data-work-id=37562975]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37562975; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37562975']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37562975, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8b7770aa69900327a7ce0d3b5b7f5688" } } $('.js-work-strip[data-work-id=37562975]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37562975,"title":"Hope and Cure for Treatment of Genetic / Rare Diseases with CRISPR Genome Repair Technology","translated_title":"","metadata":{"abstract":"Mostly genetic-based, approximately 7000 rare diseases have been identified globally. Most of the rare diseases affect the quality of life considerably. In this respect, studies on the development of trustable and efficient genetically-based treatment methods are very critical. New designed nuclease-targeted genomic regulation techniques are exciting for the biomedical research field and providing a hope for clinical applications as never before. The CRISPR/Cas system paved the way for the manipulation of gene expression or regulation and led to the questioning of gene functions in mammalian cell lines in vitro and in many different organisms in vivo including insects, plants, animals and now, humans. This genetic engineering approach is a highly efficient and scalable biotechnological tool that generates genome-wide mutations through sequence-specific target guide RNAs to induce double-stranded DNA breaks. These man-made mutations enable to fix or repair the site of disease-related genetic disorders with the help of homology-directed repair approach. Thousands of scientific publications have been recently published on CRISPR/Cas technology that demonstrates successfully repaired or altered DNA sequence in cells. Here, I will illustrate by examples how tens of genetically-based diseases are treated in the laboratory or in animal models using CRISPR/Cas genome repair technology, which pushes biotechnology and therapeutic companies to the genetic therapies.","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"The 5th International and Interdisciplinary Conference onHealth, Culture and the Human Body"},"translated_abstract":"Mostly genetic-based, approximately 7000 rare diseases have been identified globally. Most of the rare diseases affect the quality of life considerably. In this respect, studies on the development of trustable and efficient genetically-based treatment methods are very critical. 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Here, I will illustrate by examples how tens of genetically-based diseases are treated in the laboratory or in animal models using CRISPR/Cas genome repair technology, which pushes biotechnology and therapeutic companies to the genetic therapies.","internal_url":"https://www.academia.edu/37562975/Hope_and_Cure_for_Treatment_of_Genetic_Rare_Diseases_with_CRISPR_Genome_Repair_Technology","translated_internal_url":"","created_at":"2018-10-10T07:56:56.280-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[],"downloadable_attachments":[{"id":57539840,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/57539840/thumbnails/1.jpg","file_name":"abstract_4-6_Oct_2018.pdf","download_url":"https://www.academia.edu/attachments/57539840/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Hope_and_Cure_for_Treatment_of_Genetic_R.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/57539840/abstract_4-6_Oct_2018-libre.pdf?1539183649=\u0026response-content-disposition=attachment%3B+filename%3DHope_and_Cure_for_Treatment_of_Genetic_R.pdf\u0026Expires=1732704108\u0026Signature=XHvPEybc9sDe0afOo3Q-7qwMRTa~vzjfPzKO3inmjuyjeP~stmfDtOqX6T9-CJ7joIhIv6~XnlmfV1r0uD1vGyJWx~8q2PaPn9Mq3jAzTqnkixarVMAXOXlduOD98ZujQjc13MPCyAcNP7r0~rHfC~XZuMc-pvW0aGjKzCf~ZnyuOwcTWPQV2ZA3fWBCDF-RsPtBEx6dhcXSUOOpKlF4Rzj6kkFv~zj0~gZxNWkwrwSW4abDZE6n7A5gaYRoEKknWnFTvcbfSiK55juC9Id--KHbxMknv3aQvHHFrCR5RvBvjlRY9Stx~3N5v0OlR9CjpSO0I9I1-xBd28HWUWFXaQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Hope_and_Cure_for_Treatment_of_Genetic_Rare_Diseases_with_CRISPR_Genome_Repair_Technology","translated_slug":"","page_count":1,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"WkJTQ2pyL1hVMzhrcDV1bHBCTEdTTVY4d3M5ZWV3Nktwc2JJbW43ZVNVYlh3d3BhTTVwejZCcGVlang5TjlYQy0tQVdvK25NOFdKaG1JQkdaQkY2TVJ4QT09--e1d57bf64ea6b3846bf290d7e4bf6fc48109c877"},"attachments":[{"id":57539840,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/57539840/thumbnails/1.jpg","file_name":"abstract_4-6_Oct_2018.pdf","download_url":"https://www.academia.edu/attachments/57539840/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Hope_and_Cure_for_Treatment_of_Genetic_R.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/57539840/abstract_4-6_Oct_2018-libre.pdf?1539183649=\u0026response-content-disposition=attachment%3B+filename%3DHope_and_Cure_for_Treatment_of_Genetic_R.pdf\u0026Expires=1732704108\u0026Signature=XHvPEybc9sDe0afOo3Q-7qwMRTa~vzjfPzKO3inmjuyjeP~stmfDtOqX6T9-CJ7joIhIv6~XnlmfV1r0uD1vGyJWx~8q2PaPn9Mq3jAzTqnkixarVMAXOXlduOD98ZujQjc13MPCyAcNP7r0~rHfC~XZuMc-pvW0aGjKzCf~ZnyuOwcTWPQV2ZA3fWBCDF-RsPtBEx6dhcXSUOOpKlF4Rzj6kkFv~zj0~gZxNWkwrwSW4abDZE6n7A5gaYRoEKknWnFTvcbfSiK55juC9Id--KHbxMknv3aQvHHFrCR5RvBvjlRY9Stx~3N5v0OlR9CjpSO0I9I1-xBd28HWUWFXaQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy"},{"id":22379,"name":"Rare diseases","url":"https://www.academia.edu/Documents/in/Rare_diseases"},{"id":70125,"name":"CRISPR","url":"https://www.academia.edu/Documents/in/CRISPR"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="37226604"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/37226604/Fabrication_of_a_Vascularized_Tumor_Microenvironment_for_Immunotherapy"><img alt="Research paper thumbnail of Fabrication of a Vascularized Tumor Microenvironment for Immunotherapy" class="work-thumbnail" src="https://attachments.academia-assets.com/57177770/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/37226604/Fabrication_of_a_Vascularized_Tumor_Microenvironment_for_Immunotherapy">Fabrication of a Vascularized Tumor Microenvironment for Immunotherapy</a></div><div class="wp-workCard_item"><span>SOCIETY FOR BIOMATERIALS, ANNUAL MEETING & EXPOSITION</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Despite the plethora of research and advances in treatment, cancer remainsone of the le...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Despite  the  plethora  of  research and  advances  in  treatment,  cancer remainsone  of  the leading  causes  of  mortality  worldwide.  Immunotherapy for  cancer  treatment  is  an  emerging  field  of  research  but lacks  a  proper  understanding  of  cancer-immune  cell interaction  which  is  vital  to  tumor progression1. In  vitro tissue  models  that  can  recapitulatethe dynamic  immune-cancer microenvironmentare    greatly    needed    to understand these complex interactions. In this study, a 3D organoid based vascularized breast tumor microenvironment  has  been  fabricated  using  a  highly metastatic  breast  cancer  cell  line,  MDA-MB-231.  Pre-vascularized  mini  tumors  encapsulated  in  a  fibrin hydrogel  matrix  exhibited  not  only  robust  vascular network  formation  around  the  growing  tumor  but  also enabled  contiguous  internal  vascularization  of  the  tumor itself,  mimicking  native  physiology2.  Additionally, a novel method was developed using primary human T cells engineered with T  cell  receptors  (TCRs) that  can recognize  bacterial  metabolites  in  the  context  of  MR1 moleculeexpressedon the  surface  of cancer  cells, therefore  bypassing  MHC  and  antigen  requirements. These  engineered TCR+  T  cells highly effectivelyeradicated  the3D-fabricated  tumor  mass  over  three  days of  culture.  Thus,  this  system  creates  a  platform  for studying  immune-cancer  cell  interactions,  as  well  as  for anti-cancer  drug  screening  therapies  for  breast  cancer  in future</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9cc293af1ee2a02d297074b123e01ef7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":57177770,"asset_id":37226604,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/57177770/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="37226604"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="37226604"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 37226604; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=37226604]").text(description); $(".js-view-count[data-work-id=37226604]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 37226604; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='37226604']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 37226604, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9cc293af1ee2a02d297074b123e01ef7" } } $('.js-work-strip[data-work-id=37226604]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":37226604,"title":"Fabrication of a Vascularized Tumor Microenvironment for Immunotherapy","translated_title":"","metadata":{"abstract":"Despite the plethora of research and advances in treatment, cancer remainsone of the leading causes of mortality worldwide. 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Additionally, a novel method was developed using primary human T cells engineered with T cell receptors (TCRs) that can recognize bacterial metabolites in the context of MR1 moleculeexpressedon the surface of cancer cells, therefore bypassing MHC and antigen requirements. These engineered TCR+ T cells highly effectivelyeradicated the3D-fabricated tumor mass over three days of culture. Thus, this system creates a platform for studying immune-cancer cell interactions, as well as for anti-cancer drug screening therapies for breast cancer in future","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"SOCIETY FOR BIOMATERIALS, ANNUAL MEETING \u0026 EXPOSITION"},"translated_abstract":"Despite the plethora of research and advances in treatment, cancer remainsone of the leading causes of mortality worldwide. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36534418"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36534418/Hematolojik_Solid_Kanser_T%C3%BCrlerine_Kar%C5%9F%C4%B1_Do%C4%9Fal_%C3%96ld%C3%BCr%C3%BCc%C3%BC_NK92_H%C3%BCcrelerinin_Sito_Toksik_Etkinli%C4%9Fi_ve_Transgenik_Anti_CD19_CAR_NK92_%C3%9Cretimi"><img alt="Research paper thumbnail of Hematolojik/Solid Kanser Türlerine Karşı Doğal Öldürücü NK92 Hücrelerinin Sito-Toksik Etkinliği ve Transgenik Anti-CD19 CAR-NK92 Üretimi" class="work-thumbnail" src="https://attachments.academia-assets.com/56453794/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36534418/Hematolojik_Solid_Kanser_T%C3%BCrlerine_Kar%C5%9F%C4%B1_Do%C4%9Fal_%C3%96ld%C3%BCr%C3%BCc%C3%BC_NK92_H%C3%BCcrelerinin_Sito_Toksik_Etkinli%C4%9Fi_ve_Transgenik_Anti_CD19_CAR_NK92_%C3%9Cretimi">Hematolojik/Solid Kanser Türlerine Karşı Doğal Öldürücü NK92 Hücrelerinin Sito-Toksik Etkinliği ve Transgenik Anti-CD19 CAR-NK92 Üretimi</a></div><div class="wp-workCard_item"><span>V. ULUSLARARASI KATILIMLI DENEYSEL HEMATOLOJİ KONGRESİ</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Doğal Öldürücü (Naturel killer, NK) hücreler, malignant hücreleri öldürebilme özelliğinden ötürü ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Doğal Öldürücü (Naturel killer, NK) hücreler, malignant hücreleri öldürebilme özelliğinden ötürü hücresel immünoterapi için oldukça cazip hale gelmiştir. Çalışmalar allojenik NK hücre hatlarının in vivo sito-toksik fonksiyonlarını gerçekleştirebildiğini göstermiştir. GMP standartlarında kültüre edilebilir ve yüksek sayılara ulaşılabildiğinden immunoterapide birçok hasta için devamlı kaynak olarak kullanılabilmektedir (Klingemann, Frontiers in Immunology, 2016). Hâlihazırda, NK92 veya kimerik antijen reseptörü kodlayan transgenik CAR-NK92 hücreleri, anti-tümör aktivitesi için kanser hastalarında klinik çalışmalarda kullanılmaktadır (NCT00900809, NCT03383978, NCT02465957, NCT02944162, NCT00990717). Bu nedenle NK-92 aktif sito-toksik hücre hattı, satışa hazır ve standart immünoterapi ajanı olarak görülmektedir (Oelsner, Cytotherapy, 2017). Bu çalışmada NK-92 hücrelerinin hematolojik ve solid kanser türlerine karşı sito-toksik etkinliğinin değerlendirilmesi ve CD19 pozitif kanser türlerini tanıyabilecek transgenik CAR-NK92 hücreler üretilmesi amaçlanmıştır.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1641d786aad6fc21f00bbc4232569c26" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56453794,"asset_id":36534418,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56453794/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36534418"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36534418"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36534418; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36534418]").text(description); $(".js-view-count[data-work-id=36534418]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36534418; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36534418']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36534418, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1641d786aad6fc21f00bbc4232569c26" } } $('.js-work-strip[data-work-id=36534418]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36534418,"title":"Hematolojik/Solid Kanser Türlerine Karşı Doğal Öldürücü NK92 Hücrelerinin Sito-Toksik Etkinliği ve Transgenik Anti-CD19 CAR-NK92 Üretimi","translated_title":"","metadata":{"abstract":"Doğal Öldürücü (Naturel killer, NK) hücreler, malignant hücreleri öldürebilme özelliğinden ötürü hücresel immünoterapi için oldukça cazip hale gelmiştir. Çalışmalar allojenik NK hücre hatlarının in vivo sito-toksik fonksiyonlarını gerçekleştirebildiğini göstermiştir. GMP standartlarında kültüre edilebilir ve yüksek sayılara ulaşılabildiğinden immunoterapide birçok hasta için devamlı kaynak olarak kullanılabilmektedir (Klingemann, Frontiers in Immunology, 2016). Hâlihazırda, NK92 veya kimerik antijen reseptörü kodlayan transgenik CAR-NK92 hücreleri, anti-tümör aktivitesi için kanser hastalarında klinik çalışmalarda kullanılmaktadır (NCT00900809, NCT03383978, NCT02465957, NCT02944162, NCT00990717). Bu nedenle NK-92 aktif sito-toksik hücre hattı, satışa hazır ve standart immünoterapi ajanı olarak görülmektedir (Oelsner, Cytotherapy, 2017). Bu çalışmada NK-92 hücrelerinin hematolojik ve solid kanser türlerine karşı sito-toksik etkinliğinin değerlendirilmesi ve CD19 pozitif kanser türlerini tanıyabilecek transgenik CAR-NK92 hücreler üretilmesi amaçlanmıştır.","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"V. ULUSLARARASI KATILIMLI DENEYSEL HEMATOLOJİ KONGRESİ"},"translated_abstract":"Doğal Öldürücü (Naturel killer, NK) hücreler, malignant hücreleri öldürebilme özelliğinden ötürü hücresel immünoterapi için oldukça cazip hale gelmiştir. Çalışmalar allojenik NK hücre hatlarının in vivo sito-toksik fonksiyonlarını gerçekleştirebildiğini göstermiştir. GMP standartlarında kültüre edilebilir ve yüksek sayılara ulaşılabildiğinden immunoterapide birçok hasta için devamlı kaynak olarak kullanılabilmektedir (Klingemann, Frontiers in Immunology, 2016). Hâlihazırda, NK92 veya kimerik antijen reseptörü kodlayan transgenik CAR-NK92 hücreleri, anti-tümör aktivitesi için kanser hastalarında klinik çalışmalarda kullanılmaktadır (NCT00900809, NCT03383978, NCT02465957, NCT02944162, NCT00990717). Bu nedenle NK-92 aktif sito-toksik hücre hattı, satışa hazır ve standart immünoterapi ajanı olarak görülmektedir (Oelsner, Cytotherapy, 2017). Bu çalışmada NK-92 hücrelerinin hematolojik ve solid kanser türlerine karşı sito-toksik etkinliğinin değerlendirilmesi ve CD19 pozitif kanser türlerini tanıyabilecek transgenik CAR-NK92 hücreler üretilmesi amaçlanmıştır.","internal_url":"https://www.academia.edu/36534418/Hematolojik_Solid_Kanser_T%C3%BCrlerine_Kar%C5%9F%C4%B1_Do%C4%9Fal_%C3%96ld%C3%BCr%C3%BCc%C3%BC_NK92_H%C3%BCcrelerinin_Sito_Toksik_Etkinli%C4%9Fi_ve_Transgenik_Anti_CD19_CAR_NK92_%C3%9Cretimi","translated_internal_url":"","created_at":"2018-04-29T23:33:42.090-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[{"id":31374048,"work_id":36534418,"tagging_user_id":20976874,"tagged_user_id":80459237,"co_author_invite_id":null,"email":"d***i@acibademlabcell.com.tr","display_order":1,"name":"Derya Dilek Kancağı","title":"Hematolojik/Solid Kanser Türlerine Karşı Doğal Öldürücü NK92 Hücrelerinin Sito-Toksik Etkinliği ve Transgenik Anti-CD19 CAR-NK92 Üretimi"},{"id":31374049,"work_id":36534418,"tagging_user_id":20976874,"tagged_user_id":49124051,"co_author_invite_id":null,"email":"e***i@superonline.com","display_order":2,"name":"Ercument Ovali","title":"Hematolojik/Solid Kanser Türlerine Karşı Doğal Öldürücü NK92 Hücrelerinin Sito-Toksik Etkinliği ve Transgenik Anti-CD19 CAR-NK92 Üretimi"}],"downloadable_attachments":[{"id":56453794,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56453794/thumbnails/1.jpg","file_name":"Deneysel_Hematoloji_Bildiri_-_DH2018-16.pdf","download_url":"https://www.academia.edu/attachments/56453794/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Hematolojik_Solid_Kanser_Turlerine_Karsi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56453794/Deneysel_Hematoloji_Bildiri_-_DH2018-16-libre.pdf?1525070346=\u0026response-content-disposition=attachment%3B+filename%3DHematolojik_Solid_Kanser_Turlerine_Karsi.pdf\u0026Expires=1732739029\u0026Signature=H45GImrKPGjPItRuiJc-I1hVnU0caqWk5-kaB5xMZBIObmN9bzXYTazIWe8Z9yDUZFVlZyiahUo5rvBKNEtZZjdITWmNfFdMWlIKP2zgzoE1hlBSwVbHOWHHGN6AFOl0l0eUW0kBuNcN2Nak0Rj2U2mskO1AZbcnm4l4hfHISzRZXZ3SiwgR8Fno5hvTIhXUxLy7MegxLLPGygRVdDmi2naerShZdmm-x3rZkty1RuTA8JK7QPi8QqpnxZat40LtsB28LdX5y~xBhijLU5gNN6AWnsrkwZ~2-qRQvhVe5xGZ~SWnpPDaFbnidup4TW68F~~otkPRj3pWenKBHsKOtg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Hematolojik_Solid_Kanser_Türlerine_Karşı_Doğal_Öldürücü_NK92_Hücrelerinin_Sito_Toksik_Etkinliği_ve_Transgenik_Anti_CD19_CAR_NK92_Üretimi","translated_slug":"","page_count":2,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":56453794,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56453794/thumbnails/1.jpg","file_name":"Deneysel_Hematoloji_Bildiri_-_DH2018-16.pdf","download_url":"https://www.academia.edu/attachments/56453794/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Hematolojik_Solid_Kanser_Turlerine_Karsi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56453794/Deneysel_Hematoloji_Bildiri_-_DH2018-16-libre.pdf?1525070346=\u0026response-content-disposition=attachment%3B+filename%3DHematolojik_Solid_Kanser_Turlerine_Karsi.pdf\u0026Expires=1732739029\u0026Signature=H45GImrKPGjPItRuiJc-I1hVnU0caqWk5-kaB5xMZBIObmN9bzXYTazIWe8Z9yDUZFVlZyiahUo5rvBKNEtZZjdITWmNfFdMWlIKP2zgzoE1hlBSwVbHOWHHGN6AFOl0l0eUW0kBuNcN2Nak0Rj2U2mskO1AZbcnm4l4hfHISzRZXZ3SiwgR8Fno5hvTIhXUxLy7MegxLLPGygRVdDmi2naerShZdmm-x3rZkty1RuTA8JK7QPi8QqpnxZat40LtsB28LdX5y~xBhijLU5gNN6AWnsrkwZ~2-qRQvhVe5xGZ~SWnpPDaFbnidup4TW68F~~otkPRj3pWenKBHsKOtg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":22103,"name":"Cancer Immunotherapy","url":"https://www.academia.edu/Documents/in/Cancer_Immunotherapy"},{"id":555904,"name":"natural killer (NK) cell","url":"https://www.academia.edu/Documents/in/natural_killer_NK_cell"},{"id":991844,"name":"Chimeric Antigen Receptor","url":"https://www.academia.edu/Documents/in/Chimeric_Antigen_Receptor"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36299311"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36299311/CD19_Spesifik_Transgenik_CAR_T_H%C3%BCcre_U_retimi_Labcell_T%C3%BCrkiye_Verileri"><img alt="Research paper thumbnail of CD19 Spesifik Transgenik CAR-T Hücre Üretimi Labcell/Türkiye Verileri" class="work-thumbnail" src="https://attachments.academia-assets.com/56205894/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36299311/CD19_Spesifik_Transgenik_CAR_T_H%C3%BCcre_U_retimi_Labcell_T%C3%BCrkiye_Verileri">CD19 Spesifik Transgenik CAR-T Hücre Üretimi Labcell/Türkiye Verileri</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/DeryaDilekKanca%C4%9F%C4%B1">Derya Dilek Kancağı</a></span></div><div class="wp-workCard_item"><span>3. Ulusal Kan ve Kemik İliği Nakli Kongresi</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Kansere karşı hücresel immünoterapide mevcut ürünler tam remisyon oluşturmada etkili değil...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Kansere karşı hücresel immünoterapide mevcut ürünler tam remisyon oluşturmada etkili değildir. Bunun da temel nedeni, mevcut immün hücrelerin kanser dokusuna karşı uyarılmış olsalar dahi, düşük aktivite ve sensitiviteli reseptör taşımalarından ötürüdür. Bu çalışmada, modern tıpta kullanılmaya başlanan transgenik kimerik antijen reseptör (CAR)-T hücre (1- 3) üretmeyi hedefledik. Genetiği değiştirilerek hazırlanacak otolog T lenfositlerin yüksek afiniteli CD19 spesifik CAR reseptörleri taşıması sağlanarak pre- klinik/klinik anlamda daha etkin sonuçlar elde edilebilecek bir ürün geliştirmeye çalıştık.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2be85ea89e8ee098895af215958c5e2c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56205894,"asset_id":36299311,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56205894/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36299311"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36299311"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36299311; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36299311]").text(description); $(".js-view-count[data-work-id=36299311]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36299311; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36299311']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36299311, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2be85ea89e8ee098895af215958c5e2c" } } $('.js-work-strip[data-work-id=36299311]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36299311,"title":"CD19 Spesifik Transgenik CAR-T Hücre Üretimi Labcell/Türkiye Verileri","translated_title":"","metadata":{"abstract":"Kansere karşı hücresel immünoterapide mevcut ürünler tam remisyon oluşturmada etkili değildir. Bunun da temel nedeni, mevcut immün hücrelerin kanser dokusuna karşı uyarılmış olsalar dahi, düşük aktivite ve sensitiviteli reseptör taşımalarından ötürüdür. Bu çalışmada, modern tıpta kullanılmaya başlanan transgenik kimerik antijen reseptör (CAR)-T hücre (1- 3) üretmeyi hedefledik. Genetiği değiştirilerek hazırlanacak otolog T lenfositlerin yüksek afiniteli CD19 spesifik CAR reseptörleri taşıması sağlanarak pre- klinik/klinik anlamda daha etkin sonuçlar elde edilebilecek bir ürün geliştirmeye çalıştık.","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"3. Ulusal Kan ve Kemik İliği Nakli Kongresi"},"translated_abstract":"Kansere karşı hücresel immünoterapide mevcut ürünler tam remisyon oluşturmada etkili değildir. Bunun da temel nedeni, mevcut immün hücrelerin kanser dokusuna karşı uyarılmış olsalar dahi, düşük aktivite ve sensitiviteli reseptör taşımalarından ötürüdür. Bu çalışmada, modern tıpta kullanılmaya başlanan transgenik kimerik antijen reseptör (CAR)-T hücre (1- 3) üretmeyi hedefledik. Genetiği değiştirilerek hazırlanacak otolog T lenfositlerin yüksek afiniteli CD19 spesifik CAR reseptörleri taşıması sağlanarak pre- klinik/klinik anlamda daha etkin sonuçlar elde edilebilecek bir ürün geliştirmeye çalıştık.","internal_url":"https://www.academia.edu/36299311/CD19_Spesifik_Transgenik_CAR_T_H%C3%BCcre_U_retimi_Labcell_T%C3%BCrkiye_Verileri","translated_internal_url":"","created_at":"2018-03-31T13:18:39.498-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"conference_presentation","co_author_tags":[{"id":31260945,"work_id":36299311,"tagging_user_id":20976874,"tagged_user_id":80459237,"co_author_invite_id":6656758,"email":"d***i@acibademlabcell.com.tr","display_order":1,"name":"Derya Dilek Kancağı","title":"CD19 Spesifik Transgenik CAR-T Hücre Üretimi Labcell/Türkiye Verileri"},{"id":31269356,"work_id":36299311,"tagging_user_id":20976874,"tagged_user_id":49124051,"co_author_invite_id":null,"email":"e***i@superonline.com","display_order":2,"name":"Ercument Ovali","title":"CD19 Spesifik Transgenik CAR-T Hücre Üretimi Labcell/Türkiye Verileri"}],"downloadable_attachments":[{"id":56205894,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56205894/thumbnails/1.jpg","file_name":"Bildiri_Kitabi.pdf","download_url":"https://www.academia.edu/attachments/56205894/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CD19_Spesifik_Transgenik_CAR_T_Hucre_U_r.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56205894/Bildiri_Kitabi-libre.pdf?1522528028=\u0026response-content-disposition=attachment%3B+filename%3DCD19_Spesifik_Transgenik_CAR_T_Hucre_U_r.pdf\u0026Expires=1732739029\u0026Signature=MexKAmqAF9qVObMSp464l0nnDjgPslj4TGMzrUJnd5z-EJjTvQ1NpNkz9CAJJt6vDx~kK02BIvAdIPsC2kDDDV5BJ7XQXrjLUrGGJFStqWIXyMSPYDJ0vFzgmk31jnz3W0moYqLByjWrNQs3LxKySpGaFuBw3tkYAIMsEyupcVD1V4qCPyF2DJ1yzIoOKD8lOBnfOUr~Aa1eCTq4-FvJk063ecTnU1z2IGepnCW~LB8jqXNTSAXXlQhSzTz9hv5yxlynA0OGZ44HJMgGlvrbfTah1W6lTiL5OZ~VgnZGXGwHPs0vjlC4JbUlfZ2oR1q69DzUfNkl65Phi4A4E8ygwg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"CD19_Spesifik_Transgenik_CAR_T_Hücre_U_retimi_Labcell_Türkiye_Verileri","translated_slug":"","page_count":3,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":56205894,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56205894/thumbnails/1.jpg","file_name":"Bildiri_Kitabi.pdf","download_url":"https://www.academia.edu/attachments/56205894/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CD19_Spesifik_Transgenik_CAR_T_Hucre_U_r.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56205894/Bildiri_Kitabi-libre.pdf?1522528028=\u0026response-content-disposition=attachment%3B+filename%3DCD19_Spesifik_Transgenik_CAR_T_Hucre_U_r.pdf\u0026Expires=1732739029\u0026Signature=MexKAmqAF9qVObMSp464l0nnDjgPslj4TGMzrUJnd5z-EJjTvQ1NpNkz9CAJJt6vDx~kK02BIvAdIPsC2kDDDV5BJ7XQXrjLUrGGJFStqWIXyMSPYDJ0vFzgmk31jnz3W0moYqLByjWrNQs3LxKySpGaFuBw3tkYAIMsEyupcVD1V4qCPyF2DJ1yzIoOKD8lOBnfOUr~Aa1eCTq4-FvJk063ecTnU1z2IGepnCW~LB8jqXNTSAXXlQhSzTz9hv5yxlynA0OGZ44HJMgGlvrbfTah1W6lTiL5OZ~VgnZGXGwHPs0vjlC4JbUlfZ2oR1q69DzUfNkl65Phi4A4E8ygwg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":22103,"name":"Cancer Immunotherapy","url":"https://www.academia.edu/Documents/in/Cancer_Immunotherapy"},{"id":991844,"name":"Chimeric Antigen Receptor","url":"https://www.academia.edu/Documents/in/Chimeric_Antigen_Receptor"}],"urls":[]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="27873713"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/27873713/Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children"><img alt="Research paper thumbnail of Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children" class="work-thumbnail" src="https://attachments.academia-assets.com/48204095/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/27873713/Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children">Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated"> A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and<br />recognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal<br />derived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children.<br />We sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e6706a0fd1dc80c6707f16b83f91082b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":48204095,"asset_id":27873713,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/48204095/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="27873713"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="27873713"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27873713; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27873713]").text(description); $(".js-view-count[data-work-id=27873713]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27873713; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='27873713']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 27873713, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e6706a0fd1dc80c6707f16b83f91082b" } } $('.js-work-strip[data-work-id=27873713]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":27873713,"title":"Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children","translated_title":"","metadata":{"abstract":" A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and\nrecognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal\nderived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children.\nWe sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children."},"translated_abstract":" A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and\nrecognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal\nderived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children.\nWe sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ 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href="https://www.academia.edu/44766824/DNA_Tabanl%C4%B1_Gelecek_and_Biyoend%C3%BCstri_2_0">DNA Tabanlı Gelecek & Biyoendüstri 2.0</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">İEEE YTÜ EMB BİYOFORM 2020</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d2330d882182685b07776cc1b29e4ad0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":65257295,"asset_id":44766824,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/65257295/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" 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/></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/40855670/CRISPR_Cas_Antimikrobiyaller">CRISPR-Cas Antimikrobiyaller</a></div><div class="wp-workCard_item"><span>5.Ulusal Klinik Mikrobiyoloji Kongresi</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mevcut antibiyotik stratejileri spesifik olmayıp; dirençli bakteriler, direnç geni olmayan bakter...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mevcut antibiyotik stratejileri spesifik olmayıp; dirençli bakteriler, direnç geni olmayan bakterilerin çoğalmasına izin vererek direnç genlerinin bakteri popülasyonunda yayılmalarını sağlamaktadır. Ancak, genetik mühendislik teknolojilerinin gelişmesiyle birlikte özellikle virülans veya antibiyotiğe dirençli bakterileri hedef alabilecek yeni antimikrobiyal silahlar üretilebilir. Bunların başında, bakterilerin bakteriyofaj virüslerine karşı sahip oldukları adaptif immün sistem olarak tanımlanan CRISPR-Cas mekanizması gelmektedir. Bu nedenle, CRISPR-Cas antimikrobiyalleri, bakterilere karşı en yeni savunma hattı olabileceği düşünülmektedir.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="12a151c4e3d9d62e4c80f85057de858d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61141945,"asset_id":40855670,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61141945/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="40855670"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="40855670"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 40855670; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=40855670]").text(description); $(".js-view-count[data-work-id=40855670]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 40855670; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='40855670']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 40855670, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "12a151c4e3d9d62e4c80f85057de858d" } } $('.js-work-strip[data-work-id=40855670]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":40855670,"title":"CRISPR-Cas Antimikrobiyaller","translated_title":"","metadata":{"abstract":"Mevcut antibiyotik stratejileri spesifik olmayıp; dirençli bakteriler, direnç geni olmayan bakterilerin çoğalmasına izin vererek direnç genlerinin bakteri popülasyonunda yayılmalarını sağlamaktadır. Ancak, genetik mühendislik teknolojilerinin gelişmesiyle birlikte özellikle virülans veya antibiyotiğe dirençli bakterileri hedef alabilecek yeni antimikrobiyal silahlar üretilebilir. Bunların başında, bakterilerin bakteriyofaj virüslerine karşı sahip oldukları adaptif immün sistem olarak tanımlanan CRISPR-Cas mekanizması gelmektedir. Bu nedenle, CRISPR-Cas antimikrobiyalleri, bakterilere karşı en yeni savunma hattı olabileceği düşünülmektedir.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"5.Ulusal Klinik Mikrobiyoloji Kongresi"},"translated_abstract":"Mevcut antibiyotik stratejileri spesifik olmayıp; dirençli bakteriler, direnç geni olmayan bakterilerin çoğalmasına izin vererek direnç genlerinin bakteri popülasyonunda yayılmalarını sağlamaktadır. Ancak, genetik mühendislik teknolojilerinin gelişmesiyle birlikte özellikle virülans veya antibiyotiğe dirençli bakterileri hedef alabilecek yeni antimikrobiyal silahlar üretilebilir. Bunların başında, bakterilerin bakteriyofaj virüslerine karşı sahip oldukları adaptif immün sistem olarak tanımlanan CRISPR-Cas mekanizması gelmektedir. Bu nedenle, CRISPR-Cas antimikrobiyalleri, bakterilere karşı en yeni savunma hattı olabileceği düşünülmektedir.","internal_url":"https://www.academia.edu/40855670/CRISPR_Cas_Antimikrobiyaller","translated_internal_url":"","created_at":"2019-11-06T01:10:35.994-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"conference_presentation","co_author_tags":[],"downloadable_attachments":[{"id":61141945,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/61141945/thumbnails/1.jpg","file_name":"CRISPR-Cas_Antimikrobiyaller.sunum.v420191106-90032-zvvm1w.pdf","download_url":"https://www.academia.edu/attachments/61141945/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CRISPR_Cas_Antimikrobiyaller.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/61141945/CRISPR-Cas_Antimikrobiyaller.sunum.v420191106-90032-zvvm1w-libre.pdf?1573033164=\u0026response-content-disposition=attachment%3B+filename%3DCRISPR_Cas_Antimikrobiyaller.pdf\u0026Expires=1732739030\u0026Signature=C1GWEjIjhUnGGfxktRaPhxZPcQRPOVZ5KszrC8KP6aRrEJ3uvdmIi~W4vHsfYa4Yz8hn0DjBmM3lPWrCIJxamwNZ4iUVpzAukjA6MZeVNuAcPYfxVg2EPkxoxg6SLHr7aqbsCYqUwIoVUALzAeHtIIKVWqBrXIPSMsSd6bNK4GtMvtg9notzWDNh5JjpBYvTyci15qK3nyaDXXLcYgxY3-5JHz1Qk5fCzf8W77onABGR4Bv2P6tG5rkP-JXKEmG6daqifq565MAog30N1qrE8AAlUR0hZ0bbRxlgLJIeqbeB9xwfwj041FqhqgyDMJThRkK~a3yEnMdRmVxgkZFMwQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"CRISPR_Cas_Antimikrobiyaller","translated_slug":"","page_count":14,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":61141945,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/61141945/thumbnails/1.jpg","file_name":"CRISPR-Cas_Antimikrobiyaller.sunum.v420191106-90032-zvvm1w.pdf","download_url":"https://www.academia.edu/attachments/61141945/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"CRISPR_Cas_Antimikrobiyaller.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/61141945/CRISPR-Cas_Antimikrobiyaller.sunum.v420191106-90032-zvvm1w-libre.pdf?1573033164=\u0026response-content-disposition=attachment%3B+filename%3DCRISPR_Cas_Antimikrobiyaller.pdf\u0026Expires=1732739030\u0026Signature=C1GWEjIjhUnGGfxktRaPhxZPcQRPOVZ5KszrC8KP6aRrEJ3uvdmIi~W4vHsfYa4Yz8hn0DjBmM3lPWrCIJxamwNZ4iUVpzAukjA6MZeVNuAcPYfxVg2EPkxoxg6SLHr7aqbsCYqUwIoVUALzAeHtIIKVWqBrXIPSMsSd6bNK4GtMvtg9notzWDNh5JjpBYvTyci15qK3nyaDXXLcYgxY3-5JHz1Qk5fCzf8W77onABGR4Bv2P6tG5rkP-JXKEmG6daqifq565MAog30N1qrE8AAlUR0hZ0bbRxlgLJIeqbeB9xwfwj041FqhqgyDMJThRkK~a3yEnMdRmVxgkZFMwQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":70125,"name":"CRISPR","url":"https://www.academia.edu/Documents/in/CRISPR"},{"id":1916835,"name":"CRISPR-Cas9 system","url":"https://www.academia.edu/Documents/in/CRISPR-Cas9_system"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="40855755"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/40855755/Yapay_Zeka_ve_Genetik_M%C3%BChendisli%C4%9Fi_Biyotekillik"><img alt="Research paper thumbnail of Yapay Zeka ve Genetik Mühendisliği: Biyotekillik" class="work-thumbnail" src="https://attachments.academia-assets.com/61141995/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/40855755/Yapay_Zeka_ve_Genetik_M%C3%BChendisli%C4%9Fi_Biyotekillik">Yapay Zeka ve Genetik Mühendisliği: Biyotekillik</a></div><div class="wp-workCard_item"><span>GELECEĞİN BİLİMİ FORUMU </span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mevcut makine öğrenme sistemlerinde YZ’nin kullanımı henüz dar olmasına rağmen, bu alandaki ilerl...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mevcut makine öğrenme sistemlerinde YZ’nin kullanımı henüz dar olmasına rağmen, bu alandaki ilerlemelerin hızı en iyimser tahminleri bile geride bırakıyor. Bilim insanları ve araştırmacılar, popüler bir tür makine öğrenme algoritmasının -sinir ağlarının- (Neural Network) daha önce hiç görülmemiş bir özelliğe sahip olduğunu keşfetti. Fütüristler ve bilim kurgu yazarları, ütopya mı yoksa distopya mı olacağına bakmaksızın YZ ve genetik mühendisliği ile doğabilecek yeni bir geleceği konu alan bolca konuları var artık. YZ ve Genetik mühendisliği, teknolojik ‘singularity’nin (tekilliğin) ne kadar heyecan verici, ürkütücü ve katma değeri yüksek bir gelişme olacağını gözler önüne sermektedir.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="eaf97c938e53c1a40522a2558888d7e7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":61141995,"asset_id":40855755,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/61141995/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="40855755"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="40855755"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 40855755; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=40855755]").text(description); $(".js-view-count[data-work-id=40855755]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 40855755; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='40855755']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 40855755, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "eaf97c938e53c1a40522a2558888d7e7" } } $('.js-work-strip[data-work-id=40855755]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":40855755,"title":"Yapay Zeka ve Genetik Mühendisliği: Biyotekillik","translated_title":"","metadata":{"abstract":"Mevcut makine öğrenme sistemlerinde YZ’nin kullanımı henüz dar olmasına rağmen, bu alandaki ilerlemelerin hızı en iyimser tahminleri bile geride bırakıyor. Bilim insanları ve araştırmacılar, popüler bir tür makine öğrenme algoritmasının -sinir ağlarının- (Neural Network) daha önce hiç görülmemiş bir özelliğe sahip olduğunu keşfetti. Fütüristler ve bilim kurgu yazarları, ütopya mı yoksa distopya mı olacağına bakmaksızın YZ ve genetik mühendisliği ile doğabilecek yeni bir geleceği konu alan bolca konuları var artık. YZ ve Genetik mühendisliği, teknolojik ‘singularity’nin (tekilliğin) ne kadar heyecan verici, ürkütücü ve katma değeri yüksek bir gelişme olacağını gözler önüne sermektedir.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"GELECEĞİN BİLİMİ FORUMU "},"translated_abstract":"Mevcut makine öğrenme sistemlerinde YZ’nin kullanımı henüz dar olmasına rağmen, bu alandaki ilerlemelerin hızı en iyimser tahminleri bile geride bırakıyor. Bilim insanları ve araştırmacılar, popüler bir tür makine öğrenme algoritmasının -sinir ağlarının- (Neural Network) daha önce hiç görülmemiş bir özelliğe sahip olduğunu keşfetti. Fütüristler ve bilim kurgu yazarları, ütopya mı yoksa distopya mı olacağına bakmaksızın YZ ve genetik mühendisliği ile doğabilecek yeni bir geleceği konu alan bolca konuları var artık. YZ ve Genetik mühendisliği, teknolojik ‘singularity’nin (tekilliğin) ne kadar heyecan verici, ürkütücü ve katma değeri yüksek bir gelişme olacağını gözler önüne sermektedir.","internal_url":"https://www.academia.edu/40855755/Yapay_Zeka_ve_Genetik_M%C3%BChendisli%C4%9Fi_Biyotekillik","translated_internal_url":"","created_at":"2019-11-06T01:15:10.510-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"conference_presentation","co_author_tags":[],"downloadable_attachments":[{"id":61141995,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/61141995/thumbnails/1.jpg","file_name":"Yapay_Zeka_ve_GM.v220191106-58454-f5gp3c.pdf","download_url":"https://www.academia.edu/attachments/61141995/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Yapay_Zeka_ve_Genetik_Muhendisligi_Biyot.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/61141995/Yapay_Zeka_ve_GM.v220191106-58454-f5gp3c-libre.pdf?1573033176=\u0026response-content-disposition=attachment%3B+filename%3DYapay_Zeka_ve_Genetik_Muhendisligi_Biyot.pdf\u0026Expires=1732739030\u0026Signature=OZsU4DTqHvOK6hxPydj0Xft3Z7Y13qimVJTdeOygNCbJRshCN2kkJPM0P60JZwFzmZ5yyviN-4KhBUy4v4e5hBuBgipkqvQJCbvhAScf2ZSNdA1cedH9N0~k2InNpQOUSgzr7kypMgVw9mvkib3OAIm8krKRC4yu0hwd4KpEjYN5vWHKzdM4vipLrZapWqE5Lxk-lvkG1fvK9SOGxfg7HoMyiUY48Qqfd0mTi6ftrmGuV3t9aIKv-ehc77jHyGnRQyYxFOtZ896ToJu9ycjUzdowoAAGnjTBsYUDT~nCbNayWn-QR-yhJK7u8kNFbQKKUIydb2bWP7rmq5BgLP~Tag__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Yapay_Zeka_ve_Genetik_Mühendisliği_Biyotekillik","translated_slug":"","page_count":20,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":61141995,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/61141995/thumbnails/1.jpg","file_name":"Yapay_Zeka_ve_GM.v220191106-58454-f5gp3c.pdf","download_url":"https://www.academia.edu/attachments/61141995/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Yapay_Zeka_ve_Genetik_Muhendisligi_Biyot.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/61141995/Yapay_Zeka_ve_GM.v220191106-58454-f5gp3c-libre.pdf?1573033176=\u0026response-content-disposition=attachment%3B+filename%3DYapay_Zeka_ve_Genetik_Muhendisligi_Biyot.pdf\u0026Expires=1732739030\u0026Signature=OZsU4DTqHvOK6hxPydj0Xft3Z7Y13qimVJTdeOygNCbJRshCN2kkJPM0P60JZwFzmZ5yyviN-4KhBUy4v4e5hBuBgipkqvQJCbvhAScf2ZSNdA1cedH9N0~k2InNpQOUSgzr7kypMgVw9mvkib3OAIm8krKRC4yu0hwd4KpEjYN5vWHKzdM4vipLrZapWqE5Lxk-lvkG1fvK9SOGxfg7HoMyiUY48Qqfd0mTi6ftrmGuV3t9aIKv-ehc77jHyGnRQyYxFOtZ896ToJu9ycjUzdowoAAGnjTBsYUDT~nCbNayWn-QR-yhJK7u8kNFbQKKUIydb2bWP7rmq5BgLP~Tag__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":70125,"name":"CRISPR","url":"https://www.academia.edu/Documents/in/CRISPR"},{"id":1916835,"name":"CRISPR-Cas9 system","url":"https://www.academia.edu/Documents/in/CRISPR-Cas9_system"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36764350"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36764350/Genetik_Hastal%C4%B1klarda_CRISPR_Genom_Tamiri"><img alt="Research paper thumbnail of Genetik Hastalıklarda CRISPR Genom Tamiri" class="work-thumbnail" src="https://attachments.academia-assets.com/56711452/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36764350/Genetik_Hastal%C4%B1klarda_CRISPR_Genom_Tamiri">Genetik Hastalıklarda CRISPR Genom Tamiri</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">2013 yılında kolay, ucuz ve yüksek ulaşılabilirlikte CRISPR genom modifikasyon tekniğinin gelişti...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">2013 yılında kolay, ucuz ve yüksek ulaşılabilirlikte CRISPR genom modifikasyon tekniğinin geliştirilmesiyle, insan hücrelerinin genetiğinin tamir edilebileceğini gösteren bilimsel makaleler yayınlanıncaya dek. Bu vakitten sonra ilaç şirketleri AR-GE çalışmalarını genetik tedaviler üzerine yoğunlaştırmaya başladı. Birçok biyoteknoloji şirketi nadir ve halk arasında tedavi edilmesi olanaksız hastalıkların gen terapi metotlarıyla tamir etmek üzerine amansız bir yarışa atıldı.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d39db35b1aaec09950b05e665af8e313" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56711452,"asset_id":36764350,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56711452/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36764350"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36764350"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36764350; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36764350]").text(description); $(".js-view-count[data-work-id=36764350]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36764350; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36764350']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36764350, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d39db35b1aaec09950b05e665af8e313" } } $('.js-work-strip[data-work-id=36764350]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36764350,"title":"Genetik Hastalıklarda CRISPR Genom Tamiri","translated_title":"","metadata":{"abstract":"2013 yılında kolay, ucuz ve yüksek ulaşılabilirlikte CRISPR genom modifikasyon tekniğinin geliştirilmesiyle, insan hücrelerinin genetiğinin tamir edilebileceğini gösteren bilimsel makaleler yayınlanıncaya dek. Bu vakitten sonra ilaç şirketleri AR-GE çalışmalarını genetik tedaviler üzerine yoğunlaştırmaya başladı. Birçok biyoteknoloji şirketi nadir ve halk arasında tedavi edilmesi olanaksız hastalıkların gen terapi metotlarıyla tamir etmek üzerine amansız bir yarışa atıldı."},"translated_abstract":"2013 yılında kolay, ucuz ve yüksek ulaşılabilirlikte CRISPR genom modifikasyon tekniğinin geliştirilmesiyle, insan hücrelerinin genetiğinin tamir edilebileceğini gösteren bilimsel makaleler yayınlanıncaya dek. Bu vakitten sonra ilaç şirketleri AR-GE çalışmalarını genetik tedaviler üzerine yoğunlaştırmaya başladı. Birçok biyoteknoloji şirketi nadir ve halk arasında tedavi edilmesi olanaksız hastalıkların gen terapi metotlarıyla tamir etmek üzerine amansız bir yarışa atıldı.","internal_url":"https://www.academia.edu/36764350/Genetik_Hastal%C4%B1klarda_CRISPR_Genom_Tamiri","translated_internal_url":"","created_at":"2018-06-02T06:09:46.688-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"book","co_author_tags":[],"downloadable_attachments":[{"id":56711452,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56711452/thumbnails/1.jpg","file_name":"CRISPR_Solutions_.pdf","download_url":"https://www.academia.edu/attachments/56711452/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genetik_Hastaliklarda_CRISPR_Genom_Tamir.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56711452/CRISPR_Solutions_-libre.pdf?1527945286=\u0026response-content-disposition=attachment%3B+filename%3DGenetik_Hastaliklarda_CRISPR_Genom_Tamir.pdf\u0026Expires=1732739030\u0026Signature=EaerGNUlc06Jzx6QQLDtQOtPdQLe8BDiPh18dWaydxV8qCXXhwZ06SUbubclIhHsbjFcHU5Q~WaiqX~y4ZW1ILM1gF3vkxKIgmjHnYKp593Wq9lLS8nJBSbV9z4rK64bVY6JvAHhL9QAY-atzaJAEXvmxfnuP-wkX1gu0WkNMvnRZEH-nRclUNRKri9Q-bV3bQZOkTo8r0kt3nV9Ph6SlpKSaknmV4rhu7UV86GoVK1fh9zzqCFRZheAWZb9c7OABtAQdoRoZWGpr7vuiPkuy67x0-XqinzSV7tCA3aKxOlKks21sRF2BAN2l0Pt9D~WaMc9PxohxL5TrD3bKeRwyg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Genetik_Hastalıklarda_CRISPR_Genom_Tamiri","translated_slug":"","page_count":25,"language":"tr","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":56711452,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56711452/thumbnails/1.jpg","file_name":"CRISPR_Solutions_.pdf","download_url":"https://www.academia.edu/attachments/56711452/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genetik_Hastaliklarda_CRISPR_Genom_Tamir.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56711452/CRISPR_Solutions_-libre.pdf?1527945286=\u0026response-content-disposition=attachment%3B+filename%3DGenetik_Hastaliklarda_CRISPR_Genom_Tamir.pdf\u0026Expires=1732739030\u0026Signature=EaerGNUlc06Jzx6QQLDtQOtPdQLe8BDiPh18dWaydxV8qCXXhwZ06SUbubclIhHsbjFcHU5Q~WaiqX~y4ZW1ILM1gF3vkxKIgmjHnYKp593Wq9lLS8nJBSbV9z4rK64bVY6JvAHhL9QAY-atzaJAEXvmxfnuP-wkX1gu0WkNMvnRZEH-nRclUNRKri9Q-bV3bQZOkTo8r0kt3nV9Ph6SlpKSaknmV4rhu7UV86GoVK1fh9zzqCFRZheAWZb9c7OABtAQdoRoZWGpr7vuiPkuy67x0-XqinzSV7tCA3aKxOlKks21sRF2BAN2l0Pt9D~WaMc9PxohxL5TrD3bKeRwyg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy"},{"id":1170194,"name":"Genetik Hastalıklar","url":"https://www.academia.edu/Documents/in/Genetik_Hastaliklar"},{"id":1614390,"name":"Biyokimya Bio Teknoloji Genetik Mühendisliği Genom Projeleri","url":"https://www.academia.edu/Documents/in/Biyokimya_Bio_Teknoloji_Genetik_Muhendisligi_Genom_Projeleri"},{"id":1916835,"name":"CRISPR-Cas9 system","url":"https://www.academia.edu/Documents/in/CRISPR-Cas9_system"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15337740"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15337740/E_CO_Sensor_with_Enhanced_Dynamic_Range_EDR_"><img alt="Research paper thumbnail of E-CO Sensor with Enhanced Dynamic Range (EDR)" class="work-thumbnail" src="https://attachments.academia-assets.com/38641008/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15337740/E_CO_Sensor_with_Enhanced_Dynamic_Range_EDR_">E-CO Sensor with Enhanced Dynamic Range (EDR)</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://monumentslab.academia.edu/SelinAkkuzu">Selin Akkuzu</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://metu.academia.edu/FundaGuzey">Funda Guzey</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://uni-bonn.academia.edu/OzkanIs">Ozkan Is</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cells can sense and respond to the presence of various gas molecules such as oxygen, nitrogen and...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cells can sense and respond to the presence of various gas molecules such as oxygen, nitrogen and carbon monoxide using gas sensor proteins. <br /><br />CooA is a carbon monoxide (CO) sensing transcription factor. It is a member of the cAMP receptor protein (CRP)/fumavate nitrate reduction (FNR) family of transcriptional regulators. CooA switches on oxidation enzymes in Rhodospirillum rubrum (a purple, nonsulfur, phototrophic bacterium) which enables the bacterium to use CO as a carbon source. <br /><br />CO is an odorless and colorless gas which can be extremely lethal. Our aim is to develop a cell sensor which can detect a wide range of CO concentration in the environment. <br /><br />We are building CooA and CooA-responsive promoter biobricks which will be transformed into E.coli. Fluorescent proteins (GFP and RFP) will be utilized as dose-responsive signals of ambient CO.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b79b9c22a42b94c38d98cfc1ad8114a1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":38641008,"asset_id":15337740,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/38641008/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15337740"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15337740"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15337740; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15337740]").text(description); $(".js-view-count[data-work-id=15337740]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15337740; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15337740']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15337740, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b79b9c22a42b94c38d98cfc1ad8114a1" } } $('.js-work-strip[data-work-id=15337740]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15337740,"title":"E-CO Sensor with Enhanced Dynamic Range (EDR)","translated_title":"","metadata":{"abstract":"Cells can sense and respond to the presence of various gas molecules such as oxygen, nitrogen and carbon monoxide using gas sensor proteins. \n\nCooA is a carbon monoxide (CO) sensing transcription factor. It is a member of the cAMP receptor protein (CRP)/fumavate nitrate reduction (FNR) family of transcriptional regulators. CooA switches on oxidation enzymes in Rhodospirillum rubrum (a purple, nonsulfur, phototrophic bacterium) which enables the bacterium to use CO as a carbon source. \n\nCO is an odorless and colorless gas which can be extremely lethal. Our aim is to develop a cell sensor which can detect a wide range of CO concentration in the environment. \n\nWe are building CooA and CooA-responsive promoter biobricks which will be transformed into E.coli. Fluorescent proteins (GFP and RFP) will be utilized as dose-responsive signals of ambient CO. "},"translated_abstract":"Cells can sense and respond to the presence of various gas molecules such as oxygen, nitrogen and carbon monoxide using gas sensor proteins. \n\nCooA is a carbon monoxide (CO) sensing transcription factor. It is a member of the cAMP receptor protein (CRP)/fumavate nitrate reduction (FNR) family of transcriptional regulators. CooA switches on oxidation enzymes in Rhodospirillum rubrum (a purple, nonsulfur, phototrophic bacterium) which enables the bacterium to use CO as a carbon source. \n\nCO is an odorless and colorless gas which can be extremely lethal. Our aim is to develop a cell sensor which can detect a wide range of CO concentration in the environment. \n\nWe are building CooA and CooA-responsive promoter biobricks which will be transformed into E.coli. Fluorescent proteins (GFP and RFP) will be utilized as dose-responsive signals of ambient CO. ","internal_url":"https://www.academia.edu/15337740/E_CO_Sensor_with_Enhanced_Dynamic_Range_EDR_","translated_internal_url":"","created_at":"2015-09-01T21:59:54.279-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"conference_presentation","co_author_tags":[{"id":7966981,"work_id":15337740,"tagging_user_id":20976874,"tagged_user_id":44307760,"co_author_invite_id":1778975,"email":"s***8@gmail.com","display_order":0,"name":"Sibel Türkkan","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"},{"id":7966982,"work_id":15337740,"tagging_user_id":20976874,"tagged_user_id":37341201,"co_author_invite_id":1778976,"email":"s***u@gmail.com","affiliation":"METU","display_order":4194304,"name":"Selin Akkuzu","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"},{"id":7966983,"work_id":15337740,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":1778977,"email":"e***7@metu.edu.tr","display_order":6291456,"name":"Serap Memik","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"},{"id":7966984,"work_id":15337740,"tagging_user_id":20976874,"tagged_user_id":52442289,"co_author_invite_id":1778978,"email":"f***y@gmail.com","affiliation":"Middle East Technical University","display_order":7340032,"name":"Funda Guzey","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"},{"id":7966986,"work_id":15337740,"tagging_user_id":20976874,"tagged_user_id":52398146,"co_author_invite_id":1778980,"email":"o***7@gmail.com","affiliation":"Rheinische Friedrich-Wilhelms-Universität Bonn","display_order":7864320,"name":"Ozkan Is","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"},{"id":17476281,"work_id":15337740,"tagging_user_id":20976874,"tagged_user_id":null,"co_author_invite_id":4007812,"email":"a***2@hotmail.com","display_order":8126464,"name":"Sibel Ataol","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"}],"downloadable_attachments":[{"id":38641008,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/38641008/thumbnails/1.jpg","file_name":"METU_Turkey.pdf","download_url":"https://www.academia.edu/attachments/38641008/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"E_CO_Sensor_with_Enhanced_Dynamic_Range.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/38641008/METU_Turkey-libre.pdf?1441224408=\u0026response-content-disposition=attachment%3B+filename%3DE_CO_Sensor_with_Enhanced_Dynamic_Range.pdf\u0026Expires=1732701728\u0026Signature=cTzjFF99VFHjkfc73mHXTwLbsxkIZMkYkfUvhxIp-U5dCZqZmXgq97~ehTjXIWMy3y1Sfp2FIXDj9NiEFIhatwYO5wm32UJWydemk-gq9n259ZvUpfSziraAq-iOmdut8eXtSWw8bVrPLB82T1mtcucjGmmrIWeAJpM1HcbuGrTWfAdE1dPM3tKg4uE2ClEeQOpt~Tip9MHTzo9tzPA1lZ2z87aLJXDJO~9ocosjDPl4uhjgu4xIkLIJbuKh2GyZNzcbyJ3xf41gl3OdQcNv9oMoSW-OrIjnTLHX7c6qfS~L34M1Fs~R~5Yz7sAbAve4ByH5VAtPlQd9ewWtwHJCQw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"E_CO_Sensor_with_Enhanced_Dynamic_Range_EDR_","translated_slug":"","page_count":26,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":38641008,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/38641008/thumbnails/1.jpg","file_name":"METU_Turkey.pdf","download_url":"https://www.academia.edu/attachments/38641008/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"E_CO_Sensor_with_Enhanced_Dynamic_Range.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/38641008/METU_Turkey-libre.pdf?1441224408=\u0026response-content-disposition=attachment%3B+filename%3DE_CO_Sensor_with_Enhanced_Dynamic_Range.pdf\u0026Expires=1732701728\u0026Signature=cTzjFF99VFHjkfc73mHXTwLbsxkIZMkYkfUvhxIp-U5dCZqZmXgq97~ehTjXIWMy3y1Sfp2FIXDj9NiEFIhatwYO5wm32UJWydemk-gq9n259ZvUpfSziraAq-iOmdut8eXtSWw8bVrPLB82T1mtcucjGmmrIWeAJpM1HcbuGrTWfAdE1dPM3tKg4uE2ClEeQOpt~Tip9MHTzo9tzPA1lZ2z87aLJXDJO~9ocosjDPl4uhjgu4xIkLIJbuKh2GyZNzcbyJ3xf41gl3OdQcNv9oMoSW-OrIjnTLHX7c6qfS~L34M1Fs~R~5Yz7sAbAve4ByH5VAtPlQd9ewWtwHJCQw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3629,"name":"Synthetic Biology","url":"https://www.academia.edu/Documents/in/Synthetic_Biology"},{"id":1317092,"name":"IGEM","url":"https://www.academia.edu/Documents/in/IGEM"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="15337812"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/15337812/Bioguide_For_Searhing_Biobricks"><img alt="Research paper thumbnail of Bioguide: For Searhing Biobricks" class="work-thumbnail" src="https://attachments.academia-assets.com/38641029/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/15337812/Bioguide_For_Searhing_Biobricks">Bioguide: For Searhing Biobricks</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">As Synthetic Biology field is on the rise, iGEM also grows up and number of parts in parts regist...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">As Synthetic Biology field is on the rise, iGEM also grows up and number of parts in parts registry increase with submission of more complex constructs each year. Our first milestone was to perform more efficient standardization on parts entry due to facing some difficulty while running our algorithms on the parts registry. We also used Software Requirements Specification, Software Design Description and Quality Plan approaches to define requirements for each part and building blocks, risks and design art elements of the designed software program.<br />Next, we have used graph theoretic modeling to visualize relations between parts and to standardize representation of the parts as much as possible. It will help us when we try to find input-output relations between either biobrick parts or constructs. By this way, our program BIOGUIDE will act as a guide for searhing biobricks and will provide alternative pathway choices to users for construction of the most reliable devices with respect to given inputs and expected outputs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="432d22966202c128fe9e52b376a35e74" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":38641029,"asset_id":15337812,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/38641029/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15337812"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15337812"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15337812; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15337812]").text(description); $(".js-view-count[data-work-id=15337812]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15337812; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15337812']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15337812, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "432d22966202c128fe9e52b376a35e74" } } $('.js-work-strip[data-work-id=15337812]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15337812,"title":"Bioguide: For Searhing Biobricks","translated_title":"","metadata":{"abstract":"As Synthetic Biology field is on the rise, iGEM also grows up and number of parts in parts registry increase with submission of more complex constructs each year. 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By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose.By this wound covering, we will fasten the healing process, and protect the wounded area from infectious agents.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8e7d926f96d824b6d8887838259b27dc" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":38641036,"asset_id":15337828,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/38641036/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="15337828"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="15337828"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15337828; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15337828]").text(description); $(".js-view-count[data-work-id=15337828]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 15337828; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='15337828']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 15337828, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8e7d926f96d824b6d8887838259b27dc" } } $('.js-work-strip[data-work-id=15337828]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":15337828,"title":"WOUND DRESSING : A FAST HEALING PROJECT","translated_title":"","metadata":{"abstract":"In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose.By this wound covering, we will fasten the healing process, and protect the wounded area from infectious agents."},"translated_abstract":"In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="5680785" id="posters"><div class="js-work-strip profile--work_container" data-work-id="43823066"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/43823066/Preclinical_Assessment_of_Efficacy_and_Safety_Analysis_of_CAR_T_Cells_ISIKOK_19_targeting_CD19_expressing_B_Cells_for_the_First_Turkish_Academic_Clinical_Trial_with_Relapsed_refractory_ALL_and_NHL_Patients"><img alt="Research paper thumbnail of Preclinical Assessment of Efficacy and Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients" class="work-thumbnail" src="https://attachments.academia-assets.com/64141589/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/43823066/Preclinical_Assessment_of_Efficacy_and_Safety_Analysis_of_CAR_T_Cells_ISIKOK_19_targeting_CD19_expressing_B_Cells_for_the_First_Turkish_Academic_Clinical_Trial_with_Relapsed_refractory_ALL_and_NHL_Patients">Preclinical Assessment of Efficacy and Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients</a></div><div class="wp-workCard_item"><span>2nd European CAR T Cell Meeting</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Relapse and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin Ly...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Relapse and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin Lymphoma (NHL) are the most studied areas in hematological cancers. Treatment of these malignancies has undergone recent transformation with the development of new gene therapy and molecular biology techniques, which are safer and well-tolerated therapeutic approaches. The CD19 antigen is the most studied therapeutic target in these hematologic cancers. For this purpose, we used a lentiviral vector encoding the CD19 antigenspecific antibody head (FMC63) conjugated with CD8-CD28-CD3ζ sequence as a chimeric antigen receptor (CAR) along with a truncated form of EGFR (EGFRt) on human T lymphocytes (CAR-T). Here, we pre-clinically assessed efficacy and safety analysis of our CART cells, namely ISIKOK-19, both in vitro and in vivo. Also, the CAR T cells demonstrated a significantly high level of cytotoxicity specifically against a CD19+ B-lymphocyte cancer model, RAJI cells, in NOD SCID mice, which lead us to the first academic practice as the clinical trial with ALL and NHL patients in Turkey.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5ef40209e302e7befc1cf747c5a8ac47" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":64141589,"asset_id":43823066,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/64141589/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="43823066"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="43823066"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 43823066; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=43823066]").text(description); $(".js-view-count[data-work-id=43823066]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 43823066; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='43823066']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 43823066, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5ef40209e302e7befc1cf747c5a8ac47" } } $('.js-work-strip[data-work-id=43823066]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":43823066,"title":"Preclinical Assessment of Efficacy and Safety Analysis of CAR-T Cells (ISIKOK-19) targeting CD19-expressing B Cells for the First Turkish Academic Clinical Trial with Relapsed/refractory ALL and NHL Patients","translated_title":"","metadata":{"doi":"10.13140/RG.2.2.12187.69921","abstract":"Relapse and refractory CD19 positive B-cell acute lymphoblastic leukemia (ALL) and Non-Hodgkin Lymphoma (NHL) are the most studied areas in hematological cancers. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="27913594"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/27913594/Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children"><img alt="Research paper thumbnail of Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children" class="work-thumbnail" src="https://attachments.academia-assets.com/48204545/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/27913594/Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children">Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), a...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and<br />recognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal<br />derived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children.<br />We sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="521d16002701505baa73460260701895" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":48204545,"asset_id":27913594,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/48204545/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="27913594"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="27913594"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27913594; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27913594]").text(description); $(".js-view-count[data-work-id=27913594]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27913594; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='27913594']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 27913594, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "521d16002701505baa73460260701895" } } $('.js-work-strip[data-work-id=27913594]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":27913594,"title":"Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children","translated_title":"","metadata":{"abstract":"A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and\nrecognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal\nderived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children.\nWe sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children."},"translated_abstract":"A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and\nrecognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal\nderived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children.\nWe sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children.","internal_url":"https://www.academia.edu/27913594/Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children","translated_internal_url":"","created_at":"2016-08-20T13:18:55.899-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":48204545,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/48204545/thumbnails/1.jpg","file_name":"AKhaitan.CROI2015.poster.final.pdf","download_url":"https://www.academia.edu/attachments/48204545/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Disruption_of_Innate_Like_Unconventional.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/48204545/AKhaitan.CROI2015.poster.final-libre.pdf?1471724727=\u0026response-content-disposition=attachment%3B+filename%3DDisruption_of_Innate_Like_Unconventional.pdf\u0026Expires=1732739030\u0026Signature=TxxBb10XUG2G3i6G3uaflORw4evhXCXiskABADUVCWtHFkKJ-2PM6RR-L1afNytMK5mYP1wpblc65u2neqVLWvvZvxFR~jNO9eK82~D4SlAH5J1VimoZU64P~Yoq8BC~Q4MFju~yKuctM0scDMBu~CAPDqqROJ88ZPMjTEPSDL0RBJe6uuuttZO53dVTT0rVznGbdtSo8lMVXiJ4dTV-kxIkED6JwL9JgbTqvjRjV16yh6dhhsEsI0QQYiVAZXHsZCTLaNeueiQDH6AaIPcQ-~2C-HQTcqIaXvCuvBng6PECKPSPKwjHCvSTmP3OEjm0O458ok5a4tsJut6Ak5XQMQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children","translated_slug":"","page_count":1,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":48204545,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/48204545/thumbnails/1.jpg","file_name":"AKhaitan.CROI2015.poster.final.pdf","download_url":"https://www.academia.edu/attachments/48204545/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Disruption_of_Innate_Like_Unconventional.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/48204545/AKhaitan.CROI2015.poster.final-libre.pdf?1471724727=\u0026response-content-disposition=attachment%3B+filename%3DDisruption_of_Innate_Like_Unconventional.pdf\u0026Expires=1732739030\u0026Signature=TxxBb10XUG2G3i6G3uaflORw4evhXCXiskABADUVCWtHFkKJ-2PM6RR-L1afNytMK5mYP1wpblc65u2neqVLWvvZvxFR~jNO9eK82~D4SlAH5J1VimoZU64P~Yoq8BC~Q4MFju~yKuctM0scDMBu~CAPDqqROJ88ZPMjTEPSDL0RBJe6uuuttZO53dVTT0rVznGbdtSo8lMVXiJ4dTV-kxIkED6JwL9JgbTqvjRjV16yh6dhhsEsI0QQYiVAZXHsZCTLaNeueiQDH6AaIPcQ-~2C-HQTcqIaXvCuvBng6PECKPSPKwjHCvSTmP3OEjm0O458ok5a4tsJut6Ak5XQMQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":4814,"name":"HIV/AIDS","url":"https://www.academia.edu/Documents/in/HIV_AIDS"},{"id":49484,"name":"Clinical immunology","url":"https://www.academia.edu/Documents/in/Clinical_immunology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="27913657"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/27913657/E_CO_Sensor_with_Enhanced_Dynamic_Range_EDR_"><img alt="Research paper thumbnail of E-CO Sensor with Enhanced Dynamic Range (EDR)" class="work-thumbnail" src="https://attachments.academia-assets.com/48204603/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/27913657/E_CO_Sensor_with_Enhanced_Dynamic_Range_EDR_">E-CO Sensor with Enhanced Dynamic Range (EDR)</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://uskudar.academia.edu/CihanTastan">Cihan Tastan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://metu.academia.edu/FundaGuzey">Funda Guzey</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://uni-bonn.academia.edu/OzkanIs">Ozkan Is</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cells can sense and respond to the presence of various gas molecules such as oxygen, nitrogen and...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cells can sense and respond to the presence of various gas molecules such as oxygen, nitrogen and carbon monoxide using gas sensor proteins. CooA is a carbon monoxide (CO) sensing transcription factor. It is a member of the cAMP receptor protein (CRP)/fumavate nitrate reduction (FNR) family of transcriptional regulators. CooA switches on oxidation enzymes in Rhodospirillum rubrum which enables the bacterium to use CO as a carbon source. CO is an odorless and colorless gas which can be extremely lethal. Our aim is to develop a cell sensor which can detect a wide range of CO concentration in the environment. We are building CooA and CooA-responsive promoter biobricks which will be transformed into E.coli. Fluorescent proteins (GFP and RFP) will be utilized as dose-responsive signals of ambient CO. Crystal structure of R.rubrum carbon monoxide sensing transcriptional activator; CooA (PDB: 1FT9)</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5a5b2abe51c2cfb0c2365638681e6934" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":48204603,"asset_id":27913657,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/48204603/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="27913657"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="27913657"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27913657; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27913657]").text(description); $(".js-view-count[data-work-id=27913657]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27913657; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='27913657']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 27913657, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5a5b2abe51c2cfb0c2365638681e6934" } } $('.js-work-strip[data-work-id=27913657]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":27913657,"title":"E-CO Sensor with Enhanced Dynamic Range (EDR)","translated_title":"","metadata":{"abstract":"Cells can sense and respond to the presence of various gas molecules such as oxygen, nitrogen and carbon monoxide using gas sensor proteins. CooA is a carbon monoxide (CO) sensing transcription factor. It is a member of the cAMP receptor protein (CRP)/fumavate nitrate reduction (FNR) family of transcriptional regulators. CooA switches on oxidation enzymes in Rhodospirillum rubrum which enables the bacterium to use CO as a carbon source. CO is an odorless and colorless gas which can be extremely lethal. Our aim is to develop a cell sensor which can detect a wide range of CO concentration in the environment. We are building CooA and CooA-responsive promoter biobricks which will be transformed into E.coli. Fluorescent proteins (GFP and RFP) will be utilized as dose-responsive signals of ambient CO. Crystal structure of R.rubrum carbon monoxide sensing transcriptional activator; CooA (PDB: 1FT9)"},"translated_abstract":"Cells can sense and respond to the presence of various gas molecules such as oxygen, nitrogen and carbon monoxide using gas sensor proteins. CooA is a carbon monoxide (CO) sensing transcription factor. It is a member of the cAMP receptor protein (CRP)/fumavate nitrate reduction (FNR) family of transcriptional regulators. CooA switches on oxidation enzymes in Rhodospirillum rubrum which enables the bacterium to use CO as a carbon source. CO is an odorless and colorless gas which can be extremely lethal. Our aim is to develop a cell sensor which can detect a wide range of CO concentration in the environment. We are building CooA and CooA-responsive promoter biobricks which will be transformed into E.coli. Fluorescent proteins (GFP and RFP) will be utilized as dose-responsive signals of ambient CO. Crystal structure of R.rubrum carbon monoxide sensing transcriptional activator; CooA (PDB: 1FT9)","internal_url":"https://www.academia.edu/27913657/E_CO_Sensor_with_Enhanced_Dynamic_Range_EDR_","translated_internal_url":"","created_at":"2016-08-20T13:28:14.047-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[{"id":23605255,"work_id":27913657,"tagging_user_id":20976874,"tagged_user_id":37341201,"co_author_invite_id":null,"email":"s***u@gmail.com","affiliation":"METU","display_order":0,"name":"Selin Akkuzu","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"},{"id":23605256,"work_id":27913657,"tagging_user_id":20976874,"tagged_user_id":52442289,"co_author_invite_id":1778978,"email":"f***y@gmail.com","affiliation":"Middle East Technical University","display_order":4194304,"name":"Funda Guzey","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"},{"id":23605257,"work_id":27913657,"tagging_user_id":20976874,"tagged_user_id":52398146,"co_author_invite_id":1778980,"email":"o***7@gmail.com","affiliation":"Rheinische Friedrich-Wilhelms-Universität Bonn","display_order":6291456,"name":"Ozkan Is","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"},{"id":23605258,"work_id":27913657,"tagging_user_id":20976874,"tagged_user_id":76533522,"co_author_invite_id":4063793,"email":"s***k@gmail.com","display_order":7340032,"name":"Serap Memik","title":"E-CO Sensor with Enhanced Dynamic Range (EDR)"}],"downloadable_attachments":[{"id":48204603,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/48204603/thumbnails/1.jpg","file_name":"METU_Turkey.pdf","download_url":"https://www.academia.edu/attachments/48204603/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"E_CO_Sensor_with_Enhanced_Dynamic_Range.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/48204603/METU_Turkey-libre.pdf?1471725074=\u0026response-content-disposition=attachment%3B+filename%3DE_CO_Sensor_with_Enhanced_Dynamic_Range.pdf\u0026Expires=1732739030\u0026Signature=gE9PxEKiS4ORb7YcdiBOEyUNUOFGblImHfwWFcHNw9N4lZijr4GwNDs5LB0y2g9mZ3W7dzSbP6lbE~WvGULDcbBmH2kgzrKCWegfFnYvRoxSk-Oi5ag7zflCW-DKCUdrD-JEW60VLdd1tfuRhXrBTjA5yCrSEkj7~KdZ5Ymcbv8GgBd4mpHrK9navbSrtJBk2NS2P4otOjMoWXWVFGDkzVGXlgXuOulqjAnTKP5A~UK9RP-DytF0JX3yErlK47A1qSWkyxWp6VQyz-kBI3~rfMIx-~plv2P6yhwCj9go-Rlg7nvn6~TJn616uu4SepO7lwcnAFWe5yadsGPW70rg7g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"E_CO_Sensor_with_Enhanced_Dynamic_Range_EDR_","translated_slug":"","page_count":1,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":48204603,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/48204603/thumbnails/1.jpg","file_name":"METU_Turkey.pdf","download_url":"https://www.academia.edu/attachments/48204603/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"E_CO_Sensor_with_Enhanced_Dynamic_Range.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/48204603/METU_Turkey-libre.pdf?1471725074=\u0026response-content-disposition=attachment%3B+filename%3DE_CO_Sensor_with_Enhanced_Dynamic_Range.pdf\u0026Expires=1732739031\u0026Signature=HcIwcETM9BM7QCw0p8MqNWwLaQs7xpD4dONtJV4Jsf3m1633cHmUpzuEDpyDy4sZwm0Js-ZtYMoPZCr0px~o9RGt-QwzHnClZokdLTs2rXUx6wW8u5ckx9jkc8Gbao5FOHuOPRQk3cjLwZN-yfv6Pj1rFqhREUqs3QZQThOcTVv6cqc~zR~muk7zaL84E8LsF20RhYEAnqVU9AlOo0CEwImV0f684YiMUr9d1ZuBggxkWoX-AiX28CN5VgvpI7Ku70rQStqCN7bfdXdX~dkG~1UDSfZB7wCT89Ic7kCw7IkhNKphRVGr1F4T79cynCaKFK1C4d0qzuxyiHPPvK0J0Q__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3629,"name":"Synthetic Biology","url":"https://www.academia.edu/Documents/in/Synthetic_Biology"},{"id":1317092,"name":"IGEM","url":"https://www.academia.edu/Documents/in/IGEM"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="27913619"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/27913619/Wound_Dressing_A_Fast_Healing_Mechanism"><img alt="Research paper thumbnail of Wound Dressing; A Fast Healing Mechanism" class="work-thumbnail" src="https://attachments.academia-assets.com/48204571/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/27913619/Wound_Dressing_A_Fast_Healing_Mechanism">Wound Dressing; A Fast Healing Mechanism</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus a...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible activate the cell proliferation and stimulate<br />the healing process. By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose. By this wound covering, we<br />will fasten the healing process, and protect the wounded area from infectious agents. In this wound dressing, there will be four layers including polyurethane layers and our bacteria colonies. Constructed bacteria will be capable of synthesizing human epidermal growth<br />factor and keratinocyte growth factor as benefited from the communication ensured by quorum sensing molecules.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e688cbdecd06770cf64b2b803413dd50" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":48204571,"asset_id":27913619,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/48204571/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="27913619"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="27913619"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27913619; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27913619]").text(description); $(".js-view-count[data-work-id=27913619]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27913619; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='27913619']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 27913619, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e688cbdecd06770cf64b2b803413dd50" } } $('.js-work-strip[data-work-id=27913619]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":27913619,"title":"Wound Dressing; A Fast Healing Mechanism","translated_title":"","metadata":{"abstract":"In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible activate the cell proliferation and stimulate\nthe healing process. By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose. By this wound covering, we\nwill fasten the healing process, and protect the wounded area from infectious agents. In this wound dressing, there will be four layers including polyurethane layers and our bacteria colonies. Constructed bacteria will be capable of synthesizing human epidermal growth\nfactor and keratinocyte growth factor as benefited from the communication ensured by quorum sensing molecules."},"translated_abstract":"In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible activate the cell proliferation and stimulate\nthe healing process. By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose. By this wound covering, we\nwill fasten the healing process, and protect the wounded area from infectious agents. In this wound dressing, there will be four layers including polyurethane layers and our bacteria colonies. Constructed bacteria will be capable of synthesizing human epidermal growth\nfactor and keratinocyte growth factor as benefited from the communication ensured by quorum sensing molecules.","internal_url":"https://www.academia.edu/27913619/Wound_Dressing_A_Fast_Healing_Mechanism","translated_internal_url":"","created_at":"2016-08-20T13:22:31.509-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"other","co_author_tags":[],"downloadable_attachments":[{"id":48204571,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/48204571/thumbnails/1.jpg","file_name":"METU-Gene.pdf","download_url":"https://www.academia.edu/attachments/48204571/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Wound_Dressing_A_Fast_Healing_Mechanism.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/48204571/METU-Gene-libre.pdf?1471724885=\u0026response-content-disposition=attachment%3B+filename%3DWound_Dressing_A_Fast_Healing_Mechanism.pdf\u0026Expires=1732739031\u0026Signature=U0nmHEOw7ykRd3Kwqbdaizc7lkWl0roes0ojssQgL614uCM~jWhbcM1JFWGu-7jqtWW3qlRUIt5PodDlt9pY4xxZA21egTwB7fIpaS7CN1M4PHAC~LvpGU0C6s61mBMhHLCsOHcZttgHaZkL~-4GD~Lyuvn027ya6H~szhm~3zvv-8qH3Xvfgb3tr47PQlLSG1Cl8MESM8x4Wzdqo1H8niHm1a5ac4gPa-CLtO6az1GEeczz1MvV7D7SOl6BJBuR~mYPozzGTSRKKaVg2t6D1WQpWWm38CnuQF~pHzj9hVyO7d0kgTWPv22HvRSi5ZowyJr17GrZ03aZmgxQM4KnYw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Wound_Dressing_A_Fast_Healing_Mechanism","translated_slug":"","page_count":1,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":48204571,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/48204571/thumbnails/1.jpg","file_name":"METU-Gene.pdf","download_url":"https://www.academia.edu/attachments/48204571/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Wound_Dressing_A_Fast_Healing_Mechanism.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/48204571/METU-Gene-libre.pdf?1471724885=\u0026response-content-disposition=attachment%3B+filename%3DWound_Dressing_A_Fast_Healing_Mechanism.pdf\u0026Expires=1732739031\u0026Signature=U0nmHEOw7ykRd3Kwqbdaizc7lkWl0roes0ojssQgL614uCM~jWhbcM1JFWGu-7jqtWW3qlRUIt5PodDlt9pY4xxZA21egTwB7fIpaS7CN1M4PHAC~LvpGU0C6s61mBMhHLCsOHcZttgHaZkL~-4GD~Lyuvn027ya6H~szhm~3zvv-8qH3Xvfgb3tr47PQlLSG1Cl8MESM8x4Wzdqo1H8niHm1a5ac4gPa-CLtO6az1GEeczz1MvV7D7SOl6BJBuR~mYPozzGTSRKKaVg2t6D1WQpWWm38CnuQF~pHzj9hVyO7d0kgTWPv22HvRSi5ZowyJr17GrZ03aZmgxQM4KnYw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3629,"name":"Synthetic Biology","url":"https://www.academia.edu/Documents/in/Synthetic_Biology"},{"id":1317092,"name":"IGEM","url":"https://www.academia.edu/Documents/in/IGEM"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="13810924" id="papers"><div class="js-work-strip profile--work_container" data-work-id="98931369"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/98931369/Autonomous_Remotely_Controlled_Closed_System_Transgenic_Cell_Technologies_Robot_CRISPR_BOT"><img alt="Research paper thumbnail of Autonomous Remotely Controlled Closed System Transgenic Cell Technologies Robot: CRISPR.BOT" class="work-thumbnail" src="https://attachments.academia-assets.com/100151360/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/98931369/Autonomous_Remotely_Controlled_Closed_System_Transgenic_Cell_Technologies_Robot_CRISPR_BOT">Autonomous Remotely Controlled Closed System Transgenic Cell Technologies Robot: CRISPR.BOT</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In manually advancing experimental processes, the stages may be long-term and need to be repeated...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In manually advancing experimental processes, the stages may be long-term and need to be repeated. Human errors with the repetition of the steps turn into a time-consuming and high-cost for the experiment processes. For this reason, autonomous liquid processing systems are promising technologies. However, in addition to the high cost of fully automatic systems, their maintenance is also quite expensive. Furthermore, conventional systems usually require system-specific protocols and laboratory equipment. Here, we aimed to show that the autonomous robotic systems may provide a closed and error-free molecular biology bench to perform genetic engineering automatically, quickly, and practically 7-24. In this way, researchers can save time from repetitive experiment processes and perform BSL3 experiments including pathogens without human contact. In this study, we built CRISPR.BOT robotic systems to perform Green Fluorescent Protein (GFP) encoding plasmid DNA transfer into bacteria, lenti...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a60c4d4c1a4366a52cf5045c9f4d84d7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":100151360,"asset_id":98931369,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/100151360/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="98931369"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="98931369"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 98931369; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=98931369]").text(description); $(".js-view-count[data-work-id=98931369]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 98931369; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='98931369']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 98931369, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "a60c4d4c1a4366a52cf5045c9f4d84d7" } } $('.js-work-strip[data-work-id=98931369]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":98931369,"title":"Autonomous Remotely Controlled Closed System Transgenic Cell Technologies Robot: CRISPR.BOT","translated_title":"","metadata":{"abstract":"In manually advancing experimental processes, the stages may be long-term and need to be repeated. Human errors with the repetition of the steps turn into a time-consuming and high-cost for the experiment processes. For this reason, autonomous liquid processing systems are promising technologies. However, in addition to the high cost of fully automatic systems, their maintenance is also quite expensive. Furthermore, conventional systems usually require system-specific protocols and laboratory equipment. Here, we aimed to show that the autonomous robotic systems may provide a closed and error-free molecular biology bench to perform genetic engineering automatically, quickly, and practically 7-24. In this way, researchers can save time from repetitive experiment processes and perform BSL3 experiments including pathogens without human contact. In this study, we built CRISPR.BOT robotic systems to perform Green Fluorescent Protein (GFP) encoding plasmid DNA transfer into bacteria, lenti...","publisher":"Cold Spring Harbor Laboratory"},"translated_abstract":"In manually advancing experimental processes, the stages may be long-term and need to be repeated. Human errors with the repetition of the steps turn into a time-consuming and high-cost for the experiment processes. For this reason, autonomous liquid processing systems are promising technologies. However, in addition to the high cost of fully automatic systems, their maintenance is also quite expensive. Furthermore, conventional systems usually require system-specific protocols and laboratory equipment. Here, we aimed to show that the autonomous robotic systems may provide a closed and error-free molecular biology bench to perform genetic engineering automatically, quickly, and practically 7-24. In this way, researchers can save time from repetitive experiment processes and perform BSL3 experiments including pathogens without human contact. In this study, we built CRISPR.BOT robotic systems to perform Green Fluorescent Protein (GFP) encoding plasmid DNA transfer into bacteria, lenti...","internal_url":"https://www.academia.edu/98931369/Autonomous_Remotely_Controlled_Closed_System_Transgenic_Cell_Technologies_Robot_CRISPR_BOT","translated_internal_url":"","created_at":"2023-03-22T01:30:58.181-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":100151360,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/100151360/thumbnails/1.jpg","file_name":"2022.07.22.497959.full.pdf","download_url":"https://www.academia.edu/attachments/100151360/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Autonomous_Remotely_Controlled_Closed_Sy.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/100151360/2022.07.22.497959.full-libre.pdf?1679474932=\u0026response-content-disposition=attachment%3B+filename%3DAutonomous_Remotely_Controlled_Closed_Sy.pdf\u0026Expires=1732739031\u0026Signature=Xl94jNG7H93PEl3zi--LfljjPzLbCY5Pvn2vmzy6nS6AuB6o0CrV7CRpUw~C30IHAWvJ4hrjZb68dS7EI2pjMEkgV7qDHv9RUho~qGbo3RItHHHr62EwyEz1vCqjcX-8LkzJ2iYtomB4CAgJQNTbbe1H1qiEE7Rp2agAqokH~1ARn2tnvEcMnPylMTLHIfpy1yccVMfLXMrkj9Kj8~3It-CEr~CGZgtyZjKqwzjliexFMJZ2sWBMkUkiTmDXHLB63ZuAuWcJ74vSvSY-3ql9ybXLiwMXPINo~Y7S1zZsjvO5EPd5W7PEmzpA8VyHEvhyFoxznq6qATt-YPXjUtokXg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Autonomous_Remotely_Controlled_Closed_System_Transgenic_Cell_Technologies_Robot_CRISPR_BOT","translated_slug":"","page_count":38,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[{"id":100151360,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/100151360/thumbnails/1.jpg","file_name":"2022.07.22.497959.full.pdf","download_url":"https://www.academia.edu/attachments/100151360/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Autonomous_Remotely_Controlled_Closed_Sy.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/100151360/2022.07.22.497959.full-libre.pdf?1679474932=\u0026response-content-disposition=attachment%3B+filename%3DAutonomous_Remotely_Controlled_Closed_Sy.pdf\u0026Expires=1732739031\u0026Signature=Xl94jNG7H93PEl3zi--LfljjPzLbCY5Pvn2vmzy6nS6AuB6o0CrV7CRpUw~C30IHAWvJ4hrjZb68dS7EI2pjMEkgV7qDHv9RUho~qGbo3RItHHHr62EwyEz1vCqjcX-8LkzJ2iYtomB4CAgJQNTbbe1H1qiEE7Rp2agAqokH~1ARn2tnvEcMnPylMTLHIfpy1yccVMfLXMrkj9Kj8~3It-CEr~CGZgtyZjKqwzjliexFMJZ2sWBMkUkiTmDXHLB63ZuAuWcJ74vSvSY-3ql9ybXLiwMXPINo~Y7S1zZsjvO5EPd5W7PEmzpA8VyHEvhyFoxznq6qATt-YPXjUtokXg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":422,"name":"Computer Science","url":"https://www.academia.edu/Documents/in/Computer_Science"},{"id":4892,"name":"Autonomous Robotics","url":"https://www.academia.edu/Documents/in/Autonomous_Robotics"},{"id":70125,"name":"CRISPR","url":"https://www.academia.edu/Documents/in/CRISPR"},{"id":157731,"name":"CAS","url":"https://www.academia.edu/Documents/in/CAS"}],"urls":[{"id":29989129,"url":"https://syndication.highwire.org/content/doi/10.1101/2022.07.22.497959"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="92196510"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/92196510/CAR_T_Cells_with_Phytohemagglutinin_PHA_Provide_Anti_Cancer_Capacity_with_Better_Proliferation_Rejuvenated_Effector_Memory_and_Reduced_Exhausted_T_Cell_Frequencies"><img alt="Research paper thumbnail of CAR-T Cells with Phytohemagglutinin (PHA) Provide Anti-Cancer Capacity with Better Proliferation, Rejuvenated Effector Memory, and Reduced Exhausted T Cell Frequencies" class="work-thumbnail" src="https://attachments.academia-assets.com/95269493/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/92196510/CAR_T_Cells_with_Phytohemagglutinin_PHA_Provide_Anti_Cancer_Capacity_with_Better_Proliferation_Rejuvenated_Effector_Memory_and_Reduced_Exhausted_T_Cell_Frequencies">CAR-T Cells with Phytohemagglutinin (PHA) Provide Anti-Cancer Capacity with Better Proliferation, Rejuvenated Effector Memory, and Reduced Exhausted T Cell Frequencies</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACTThe development of genetic modification techniques has led to the opening of a new era in...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACTThe development of genetic modification techniques has led to the opening of a new era in cancer treatments that have been limited to conventional treatments such as chemotherapy. Since not only cancerous cells but also healthy cells are damaged by the drugs, intensive efforts are made to develop cancer-targeted techniques. The most promising approach is genetically modified CAR-T cell therapy. The high central memory T cell (Tcm) and stem cell-like memory T cell (Tscm) ratios in the CAR-T cell population increase the effectiveness of immunotherapy. Therefore, it is important to increase the populations of CAR-expressing Tcm and Tscm cells to ensure that CAR-T cells remain long-term and have cytotoxic (anti-tumor) efficacy. In this study, we aimed to improve CAR-T cell therapy’s time-dependent efficacy and stability, increasing the survival time and reducing the probability of cancer cell growth. To increase the subpopulation of Tcm and Tscm in CAR-T cells, we investigated t...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="25271a633a4e1b2e153744d60e71bccc" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":95269493,"asset_id":92196510,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/95269493/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="92196510"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="92196510"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 92196510; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=92196510]").text(description); $(".js-view-count[data-work-id=92196510]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 92196510; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='92196510']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 92196510, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "25271a633a4e1b2e153744d60e71bccc" } } $('.js-work-strip[data-work-id=92196510]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":92196510,"title":"CAR-T Cells with Phytohemagglutinin (PHA) Provide Anti-Cancer Capacity with Better Proliferation, Rejuvenated Effector Memory, and Reduced Exhausted T Cell Frequencies","translated_title":"","metadata":{"abstract":"ABSTRACTThe development of genetic modification techniques has led to the opening of a new era in cancer treatments that have been limited to conventional treatments such as chemotherapy. Since not only cancerous cells but also healthy cells are damaged by the drugs, intensive efforts are made to develop cancer-targeted techniques. The most promising approach is genetically modified CAR-T cell therapy. The high central memory T cell (Tcm) and stem cell-like memory T cell (Tscm) ratios in the CAR-T cell population increase the effectiveness of immunotherapy. Therefore, it is important to increase the populations of CAR-expressing Tcm and Tscm cells to ensure that CAR-T cells remain long-term and have cytotoxic (anti-tumor) efficacy. In this study, we aimed to improve CAR-T cell therapy’s time-dependent efficacy and stability, increasing the survival time and reducing the probability of cancer cell growth. To increase the subpopulation of Tcm and Tscm in CAR-T cells, we investigated t...","publisher":"Cold Spring Harbor Laboratory"},"translated_abstract":"ABSTRACTThe development of genetic modification techniques has led to the opening of a new era in cancer treatments that have been limited to conventional treatments such as chemotherapy. Since not only cancerous cells but also healthy cells are damaged by the drugs, intensive efforts are made to develop cancer-targeted techniques. The most promising approach is genetically modified CAR-T cell therapy. The high central memory T cell (Tcm) and stem cell-like memory T cell (Tscm) ratios in the CAR-T cell population increase the effectiveness of immunotherapy. Therefore, it is important to increase the populations of CAR-expressing Tcm and Tscm cells to ensure that CAR-T cells remain long-term and have cytotoxic (anti-tumor) efficacy. In this study, we aimed to improve CAR-T cell therapy’s time-dependent efficacy and stability, increasing the survival time and reducing the probability of cancer cell growth. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243491"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243491/Bioguide_For_Searhing_Biobricks"><img alt="Research paper thumbnail of Bioguide For Searhing Biobricks" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243491/Bioguide_For_Searhing_Biobricks">Bioguide For Searhing Biobricks</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">As Synthetic Biology field is on the rise, iGEM also grows up and number of parts in parts regist...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">As Synthetic Biology field is on the rise, iGEM also grows up and number of parts in parts registry increase with submission of more complex constructs each year. Our first milestone was to perform more efficient standardization on parts entry due to facing some difficulty while running our algorithms on the parts registry. We also used Software Requirements Specification, Software Design Description and Quality Plan approaches to define requirements for each part and building blocks, risks and design art elements of the designed software program. Next, we have used graph theoretic modeling to visualize relations between parts and to standardize representation of the parts as much as possible. It will help us when we try to find input-output relations between either biobrick parts or constructs. 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By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose. By this wound covering, we will fasten the healing process, and protect the wounded area from infectious agents. In this wound dressing, there will be four layers including polyurethane layers and our bacteria colonies. Constructed bacteria will be capable of synthesizing human epidermal growth factor and keratinocyte growth factor as benefited from the communication ensured by quorum sensing molecules.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243490"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243490"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243490; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243490]").text(description); $(".js-view-count[data-work-id=81243490]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243490; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243490']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243490, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=81243490]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243490,"title":"Wound Dressing; a Fast Healing Mechanism","translated_title":"","metadata":{"abstract":"In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible activate the cell proliferation and stimulate the healing process. By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose. By this wound covering, we will fasten the healing process, and protect the wounded area from infectious agents. In this wound dressing, there will be four layers including polyurethane layers and our bacteria colonies. Constructed bacteria will be capable of synthesizing human epidermal growth factor and keratinocyte growth factor as benefited from the communication ensured by quorum sensing molecules.","publication_date":{"day":null,"month":null,"year":2009,"errors":{}}},"translated_abstract":"In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible activate the cell proliferation and stimulate the healing process. By this purpose, designing a wound dressing which is natural, non-toxic, and biodegradable and imitating the actual wound healing mechanism which is forming on open wounds in mammalian tissues is our main purpose. By this wound covering, we will fasten the healing process, and protect the wounded area from infectious agents. In this wound dressing, there will be four layers including polyurethane layers and our bacteria colonies. Constructed bacteria will be capable of synthesizing human epidermal growth factor and keratinocyte growth factor as benefited from the communication ensured by quorum sensing molecules.","internal_url":"https://www.academia.edu/81243490/Wound_Dressing_a_Fast_Healing_Mechanism","translated_internal_url":"","created_at":"2022-06-11T09:33:55.694-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Wound_Dressing_a_Fast_Healing_Mechanism","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[],"research_interests":[{"id":3629,"name":"Synthetic Biology","url":"https://www.academia.edu/Documents/in/Synthetic_Biology"},{"id":1317092,"name":"IGEM","url":"https://www.academia.edu/Documents/in/IGEM"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243488"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243488/Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children"><img alt="Research paper thumbnail of Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243488/Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children">Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), a...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and recognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal derived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children. We sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243488"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243488"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243488; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243488]").text(description); $(".js-view-count[data-work-id=81243488]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243488; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243488']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243488, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=81243488]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243488,"title":"Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children","translated_title":"","metadata":{"abstract":"A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and recognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal derived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children. We sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children.","publication_date":{"day":null,"month":null,"year":2015,"errors":{}}},"translated_abstract":"A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and recognition of non-peptide antigens. These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal derived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children. 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These unconventional T cell subsets can secrete pro-inflammatory cytokines or display cytotoxic activity in response to bacterial and fungal derived glycolipids or metabolites. HIV+ adults have lower MAIT and NKT and higher γδ T cells in the peripheral blood. Less is known about their changes in HIV-infected children. We sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243451"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243451"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243451; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243451]").text(description); $(".js-view-count[data-work-id=81243451]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243451; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243451']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243451, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=81243451]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243451,"title":"Disruption of Innate-Like Unconventional T-Cell Subsets in HIV-Infected Children","translated_title":"","metadata":{"abstract":"A subset of T cells comprised of mucosal associated invariant T (MAIT), natural killer T (NKT), and γδ T cells exhibit features of innate cells such as invariant TCR and recognition of non-peptide antigens. 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Less is known about their changes in HIV-infected children. We sought to determine whether innate-like T cells are disrupted in treated and untreated HIV+ children.","internal_url":"https://www.academia.edu/81243451/Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children","translated_internal_url":"","created_at":"2022-06-11T09:32:31.173-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Disruption_of_Innate_Like_Unconventional_T_Cell_Subsets_in_HIV_Infected_Children","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology"},{"id":49484,"name":"Clinical immunology","url":"https://www.academia.edu/Documents/in/Clinical_immunology"},{"id":359887,"name":"Hiv Aids","url":"https://www.academia.edu/Documents/in/Hiv_Aids-14"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243392"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243392/The_Overall_Consequence_of_Antiviral_Drugs_Given_to_Pregnant_Women_with_COVID_19"><img alt="Research paper thumbnail of The Overall Consequence of Antiviral Drugs Given to Pregnant Women with COVID-19" class="work-thumbnail" src="https://attachments.academia-assets.com/87356141/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243392/The_Overall_Consequence_of_Antiviral_Drugs_Given_to_Pregnant_Women_with_COVID_19">The Overall Consequence of Antiviral Drugs Given to Pregnant Women with COVID-19</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">During pregnancy, the anatomical structure of the respiratory system changes, and the virus trans...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">During pregnancy, the anatomical structure of the respiratory system changes, and the virus transmitted by droplets and aerosols are more easily inhaled and difficult to remove by pregnant women. Women are generally more susceptible to various pregnancy-related complications and respiratory pathogens, increasing the risk of developing adverse pregnancy and neonatal outcomes. Anecdotal evidence suggests that pregnant women do not appear to differ from the general population in terms of disease transmission, and to date, there is no evidence of vertical transmission from mother to fetus. However, in another study, it is known that members of the coronavirus family are responsible for serious complications such as miscarriage, fetal growth restriction, and congenital anomalies during pregnancy. To date, only a few studies have reported relatively higher rates of adverse birth outcomes in women affected by SARS-CoV-2 infection in late pregnancy. This literature review presents the use o...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6cc8ee2d6a1f59a3b2dc80f61ba8dec1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":87356141,"asset_id":81243392,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/87356141/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243392"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243392"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243392; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243392]").text(description); $(".js-view-count[data-work-id=81243392]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243392; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243392']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243392, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6cc8ee2d6a1f59a3b2dc80f61ba8dec1" } } $('.js-work-strip[data-work-id=81243392]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243392,"title":"The Overall Consequence of Antiviral Drugs Given to Pregnant Women with COVID-19","translated_title":"","metadata":{"abstract":"During pregnancy, the anatomical structure of the respiratory system changes, and the virus transmitted by droplets and aerosols are more easily inhaled and difficult to remove by pregnant women. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243355"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243355/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice"><img alt="Research paper thumbnail of Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice" class="work-thumbnail" src="https://attachments.academia-assets.com/87356110/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243355/Gamma_irradiated_SARS_CoV_2_vaccine_candidate_OZG_38_61_3_confers_protection_from_SARS_CoV_2_challenge_in_human_ACEII_transgenic_mice">Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development fo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A, oral polio vaccine, and smallpox proved to be reliable approaches for immunization for prolonged periods. During the pandemic, we produced an inactivated SARS-CoV-2 vaccine candidate, having the advantages of being manufactured rapidly and tested easily in comparison with recombinant vaccines. In this study, an inactivated virus vaccine that includes a gamma irradiation process for the inactivation as an alternative to classical chemical inactivation methods so that there is no extra purification required has been optimized. The vaccine candidate (OZG-38.61.3) was then applied in mice by employing the intradermal route, which decreased the requirement of a higher concentration of inactivated virus for proper immunization, unlike most of the classical inactivated vaccine treatments. Hence, the ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9f9eb05bb02605d7a7915ec6a10d7c0a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":87356110,"asset_id":81243355,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/87356110/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243355"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243355"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243355; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243355]").text(description); $(".js-view-count[data-work-id=81243355]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243355; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243355']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243355, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9f9eb05bb02605d7a7915ec6a10d7c0a" } } $('.js-work-strip[data-work-id=81243355]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243355,"title":"Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice","translated_title":"","metadata":{"abstract":"The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A, oral polio vaccine, and smallpox proved to be reliable approaches for immunization for prolonged periods. During the pandemic, we produced an inactivated SARS-CoV-2 vaccine candidate, having the advantages of being manufactured rapidly and tested easily in comparison with recombinant vaccines. In this study, an inactivated virus vaccine that includes a gamma irradiation process for the inactivation as an alternative to classical chemical inactivation methods so that there is no extra purification required has been optimized. The vaccine candidate (OZG-38.61.3) was then applied in mice by employing the intradermal route, which decreased the requirement of a higher concentration of inactivated virus for proper immunization, unlike most of the classical inactivated vaccine treatments. Hence, the ...","publisher":"Cold Spring Harbor Laboratory","publication_date":{"day":null,"month":null,"year":2020,"errors":{}}},"translated_abstract":"The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A, oral polio vaccine, and smallpox proved to be reliable approaches for immunization for prolonged periods. During the pandemic, we produced an inactivated SARS-CoV-2 vaccine candidate, having the advantages of being manufactured rapidly and tested easily in comparison with recombinant vaccines. In this study, an inactivated virus vaccine that includes a gamma irradiation process for the inactivation as an alternative to classical chemical inactivation methods so that there is no extra purification required has been optimized. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243310"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243310/Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics"><img alt="Research paper thumbnail of Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics" class="work-thumbnail" src="https://attachments.academia-assets.com/87356050/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243310/Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics">Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics</a></div><div class="wp-workCard_item"><span>Gene Therapy</span><span>, 2020</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="738af19c183092f1070e681d97dd0168" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":87356050,"asset_id":81243310,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/87356050/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243310"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243310"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243310; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "738af19c183092f1070e681d97dd0168" } } $('.js-work-strip[data-work-id=81243310]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243310,"title":"Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics","translated_title":"","metadata":{"publisher":"Springer Science and Business Media LLC","grobid_abstract":"The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV-2) (","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Gene Therapy","grobid_abstract_attachment_id":87356050},"translated_abstract":null,"internal_url":"https://www.academia.edu/81243310/Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics","translated_internal_url":"","created_at":"2022-06-11T09:30:29.883-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":87356050,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/87356050/thumbnails/1.jpg","file_name":"s41434-020-00209-7.pdf","download_url":"https://www.academia.edu/attachments/87356050/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Gene_editing_and_RNAi_approaches_for_COV.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/87356050/s41434-020-00209-7-libre.pdf?1654965761=\u0026response-content-disposition=attachment%3B+filename%3DGene_editing_and_RNAi_approaches_for_COV.pdf\u0026Expires=1732704110\u0026Signature=QKJBhwndIjhBi7pzLjxcKvDMG4rnqryavKztavE82mjQ8j~T-UfLQ-QGeUNmP8qKF~oZzrKjbfbmNYrI2yyBYdPo4cxtJyZ44uvT4~OccjhrBPeEiRfyAkp31xYGP-rxlnBfcVNkY2Toyd0hoqWRbIanK7FPHViXhkauP1ZcmPANE6wYf5Q9og5fCUcQW8LiSJ9gAztbTm8DNbDO-7aMcTcVf1akIDpuiL2jrLJhZs2kcfYbG8LalSoV3vscV~qRK0wLleARGIrMQGbV2TbnZ0KvV-iBKnUCoQi7wvdNvoIQ7ruLtDXdOyOBDmQBaQ2-PXlhAMALW3Espq95gPrqgw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Gene_editing_and_RNAi_approaches_for_COVID_19_diagnostics_and_therapeutics","translated_slug":"","page_count":16,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"Z1NkR29ITmdtM1Z1RUNVRGgraHJ1UU42Wk9idzZrd1ExdzhPbWdsNVF1a2pjNk5qSEllcmgwUXJaVVo5UUlXSS0tTkxyTXVvUkcvbS9mRk9EdUxreHpudz09--9002396d453ddd512513dd1e959c2a95394f5a60"},"attachments":[{"id":87356050,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/87356050/thumbnails/1.jpg","file_name":"s41434-020-00209-7.pdf","download_url":"https://www.academia.edu/attachments/87356050/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Gene_editing_and_RNAi_approaches_for_COV.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/87356050/s41434-020-00209-7-libre.pdf?1654965761=\u0026response-content-disposition=attachment%3B+filename%3DGene_editing_and_RNAi_approaches_for_COV.pdf\u0026Expires=1732704110\u0026Signature=QKJBhwndIjhBi7pzLjxcKvDMG4rnqryavKztavE82mjQ8j~T-UfLQ-QGeUNmP8qKF~oZzrKjbfbmNYrI2yyBYdPo4cxtJyZ44uvT4~OccjhrBPeEiRfyAkp31xYGP-rxlnBfcVNkY2Toyd0hoqWRbIanK7FPHViXhkauP1ZcmPANE6wYf5Q9og5fCUcQW8LiSJ9gAztbTm8DNbDO-7aMcTcVf1akIDpuiL2jrLJhZs2kcfYbG8LalSoV3vscV~qRK0wLleARGIrMQGbV2TbnZ0KvV-iBKnUCoQi7wvdNvoIQ7ruLtDXdOyOBDmQBaQ2-PXlhAMALW3Espq95gPrqgw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":51121,"name":"RNA interference","url":"https://www.academia.edu/Documents/in/RNA_interference"},{"id":70125,"name":"CRISPR","url":"https://www.academia.edu/Documents/in/CRISPR"},{"id":107108,"name":"Aptamers","url":"https://www.academia.edu/Documents/in/Aptamers"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":21325934,"url":"http://www.nature.com/articles/s41434-020-00209-7.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243299"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/81243299/Anti_cancer_effect_of_metformin_on_the_metastasis_and_invasion_of_primary_breast_cancer_cells_through_mediating_NF_kB_activity"><img alt="Research paper thumbnail of Anti-cancer effect of metformin on the metastasis and invasion of primary breast cancer cells through mediating NF-kB activity" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/81243299/Anti_cancer_effect_of_metformin_on_the_metastasis_and_invasion_of_primary_breast_cancer_cells_through_mediating_NF_kB_activity">Anti-cancer effect of metformin on the metastasis and invasion of primary breast cancer cells through mediating NF-kB activity</a></div><div class="wp-workCard_item"><span>Acta Histochemica</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Current evidence strongly suggests that aberrant activation of the nuclear factor kappa B (NF-kB)...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Current evidence strongly suggests that aberrant activation of the nuclear factor kappa B (NF-kB) signaling cascade is connected to carcinogenesis. The matrix metalloproteinases (MMP) which are also the key agents for tumor metastasis may be potent candidates for tumor diagnosis in clinics. In this in vitro study, we hypothesized that metformin with an effective dose can inhibit tumor cell proliferation and metastasis by modulating the expressions of MMP-2 and -9 and interfering with NF-kB signaling in primary breast cancer cells (PBCCs). 300 000 cells per ml were obtained from biopsies of breast tumors from five human donors. The cell viability and proliferation were tested. Immunocytochemistry was performed for MMP-2, MMP-9, and NF-kB, and enzyme-linked immunosorbent assay for NF-kB activity, quantitative real-time PCR for RELA/p65, IkBα, MMP-2, and MMP-9. Three different doses of metformin (5, 10, and 25 mM) (Met) reduced the viability and proliferation of PBCCs in a dose-dependent manner, maximum inhibition was observed at 25 mM Met. The expression of RELA/p65 was not affected by 25 mM Met. Nuclear immunoreactivity and activity of NF-kB reduced while cytoplasmic NF-kB (p65) elevated by 25 mM Met compared to non-treatment (P &lt;  0.05). The expression and immunoreactivity of MMP-9 but not MMP-2 were decreased by 25 mM Met treatment, compared with the non-treatment (P &lt;  0.05). Metformin may have an essential antitumor role in the invasion and metastasis pathways of PBCCs by downregulating the MMP-9 expression blocking both the activity and nuclear translocation of NF-kB.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243299"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243299"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243299; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243299]").text(description); $(".js-view-count[data-work-id=81243299]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243299; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243299']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243299, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=81243299]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243299,"title":"Anti-cancer effect of metformin on the metastasis and invasion of primary breast cancer cells through mediating NF-kB activity","translated_title":"","metadata":{"abstract":"Current evidence strongly suggests that aberrant activation of the nuclear factor kappa B (NF-kB) signaling cascade is connected to carcinogenesis. The matrix metalloproteinases (MMP) which are also the key agents for tumor metastasis may be potent candidates for tumor diagnosis in clinics. In this in vitro study, we hypothesized that metformin with an effective dose can inhibit tumor cell proliferation and metastasis by modulating the expressions of MMP-2 and -9 and interfering with NF-kB signaling in primary breast cancer cells (PBCCs). 300 000 cells per ml were obtained from biopsies of breast tumors from five human donors. The cell viability and proliferation were tested. Immunocytochemistry was performed for MMP-2, MMP-9, and NF-kB, and enzyme-linked immunosorbent assay for NF-kB activity, quantitative real-time PCR for RELA/p65, IkBα, MMP-2, and MMP-9. Three different doses of metformin (5, 10, and 25 mM) (Met) reduced the viability and proliferation of PBCCs in a dose-dependent manner, maximum inhibition was observed at 25 mM Met. The expression of RELA/p65 was not affected by 25 mM Met. Nuclear immunoreactivity and activity of NF-kB reduced while cytoplasmic NF-kB (p65) elevated by 25 mM Met compared to non-treatment (P \u0026lt;  0.05). The expression and immunoreactivity of MMP-9 but not MMP-2 were decreased by 25 mM Met treatment, compared with the non-treatment (P \u0026lt;  0.05). Metformin may have an essential antitumor role in the invasion and metastasis pathways of PBCCs by downregulating the MMP-9 expression blocking both the activity and nuclear translocation of NF-kB.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"Acta Histochemica"},"translated_abstract":"Current evidence strongly suggests that aberrant activation of the nuclear factor kappa B (NF-kB) signaling cascade is connected to carcinogenesis. The matrix metalloproteinases (MMP) which are also the key agents for tumor metastasis may be potent candidates for tumor diagnosis in clinics. In this in vitro study, we hypothesized that metformin with an effective dose can inhibit tumor cell proliferation and metastasis by modulating the expressions of MMP-2 and -9 and interfering with NF-kB signaling in primary breast cancer cells (PBCCs). 300 000 cells per ml were obtained from biopsies of breast tumors from five human donors. The cell viability and proliferation were tested. Immunocytochemistry was performed for MMP-2, MMP-9, and NF-kB, and enzyme-linked immunosorbent assay for NF-kB activity, quantitative real-time PCR for RELA/p65, IkBα, MMP-2, and MMP-9. Three different doses of metformin (5, 10, and 25 mM) (Met) reduced the viability and proliferation of PBCCs in a dose-dependent manner, maximum inhibition was observed at 25 mM Met. The expression of RELA/p65 was not affected by 25 mM Met. Nuclear immunoreactivity and activity of NF-kB reduced while cytoplasmic NF-kB (p65) elevated by 25 mM Met compared to non-treatment (P \u0026lt;  0.05). The expression and immunoreactivity of MMP-9 but not MMP-2 were decreased by 25 mM Met treatment, compared with the non-treatment (P \u0026lt;  0.05). Metformin may have an essential antitumor role in the invasion and metastasis pathways of PBCCs by downregulating the MMP-9 expression blocking both the activity and nuclear translocation of NF-kB.","internal_url":"https://www.academia.edu/81243299/Anti_cancer_effect_of_metformin_on_the_metastasis_and_invasion_of_primary_breast_cancer_cells_through_mediating_NF_kB_activity","translated_internal_url":"","created_at":"2022-06-11T09:30:10.282-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Anti_cancer_effect_of_metformin_on_the_metastasis_and_invasion_of_primary_breast_cancer_cells_through_mediating_NF_kB_activity","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[],"research_interests":[{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology"},{"id":6021,"name":"Cancer","url":"https://www.academia.edu/Documents/in/Cancer"},{"id":6802,"name":"Breast Cancer","url":"https://www.academia.edu/Documents/in/Breast_Cancer"},{"id":14195,"name":"Cancer Biology","url":"https://www.academia.edu/Documents/in/Cancer_Biology"},{"id":21936,"name":"Metastasis","url":"https://www.academia.edu/Documents/in/Metastasis"},{"id":22255,"name":"Cancer Research","url":"https://www.academia.edu/Documents/in/Cancer_Research"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":71297,"name":"Metformin","url":"https://www.academia.edu/Documents/in/Metformin"},{"id":527895,"name":"MMP","url":"https://www.academia.edu/Documents/in/MMP"},{"id":3789880,"name":"Medical biochemistry and metabolomics","url":"https://www.academia.edu/Documents/in/Medical_biochemistry_and_metabolomics"}],"urls":[{"id":21325931,"url":"https://api.elsevier.com/content/article/PII:S0065128121000313?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243276"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243276/Preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates"><img alt="Research paper thumbnail of Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates" class="work-thumbnail" src="https://attachments.academia-assets.com/87356058/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243276/Preclinical_efficacy_and_safety_analysis_of_gamma_irradiated_inactivated_SARS_CoV_2_vaccine_candidates">Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no v...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, SARS-CoV-2 vaccines have become crucial for reducing morbidity and mortality. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. The candidate vaccines in this study were OZG-3861 version 1 (V1), an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1), a GM-CSF adjuvant added vaccine. The candidate vaccines were applied intradermally to BALB/c mice to assess toxicity and immunogenicity. Preliminary results in vaccinated mice are reported in this study. Especially, the vaccine models containing GM-CSF caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature, when considered in terms of T and B cell responses. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243240"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243240/Tuning_of_human_MAIT_cell_activation_by_commensal_bacteria_species_and_MR1_dependent_T_cell_presentation"><img alt="Research paper thumbnail of Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation" class="work-thumbnail" src="https://attachments.academia-assets.com/87356028/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243240/Tuning_of_human_MAIT_cell_activation_by_commensal_bacteria_species_and_MR1_dependent_T_cell_presentation">Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation</a></div><div class="wp-workCard_item"><span>Mucosal immunology</span><span>, Jan 16, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. However, it is unclear whether MAIT cells discriminate between many species of the human microbiota. To address this, we developed an in vitro functional assay through human T cells engineered for MAIT-TCRs (eMAIT-TCRs) stimulated by MR1-expressing antigen-presenting cells (APCs). We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded the riboflavin pathway were stimulatory for MAIT-TCRs. Most species that were high stimulators belonged to Bacteroidetes and Proteobacteria phyla, whereas low/non-stimulator species were primarily Actinobacteria or Firmicutes. Activation of MAIT cells by high- vs low-stimulating bacteria also correlated with the level of riboflavin they secreted or after bacterial infection of macrophages...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5cbea960f7746cc4f529172930b9ff31" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":87356028,"asset_id":81243240,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/87356028/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243240"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243240"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243240; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243240]").text(description); $(".js-view-count[data-work-id=81243240]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243240; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243240']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243240, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5cbea960f7746cc4f529172930b9ff31" } } $('.js-work-strip[data-work-id=81243240]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243240,"title":"Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation","translated_title":"","metadata":{"abstract":"Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243231"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243231/Functional_Interrogation_of_Primary_Human_T_Cells_via_CRISPR_Genetic_Editing"><img alt="Research paper thumbnail of Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing" class="work-thumbnail" src="https://attachments.academia-assets.com/87356021/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243231/Functional_Interrogation_of_Primary_Human_T_Cells_via_CRISPR_Genetic_Editing">Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing</a></div><div class="wp-workCard_item"><span>Journal of immunology (Baltimore, Md. : 1950)</span><span>, Jan 18, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Developing precise and efficient gene editing approaches using CRISPR in primary human T cell sub...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (, , ) could be simultaneously and stably deleted in naive, memory, effector, or regulatory T cell (Treg) subsets at very high efficiency. Additionally, nuclease-deficient Cas9, associated with a transcriptional activator or repressor, can downregulate or increase expression of genes in T cells. For example, expression of glycoprotein A repetitions predominant (GARP), a gene that is normally and exclusively expressed on activated Tregs, could be induced on non-Treg effector T cells by nuclease-deficient Cas9 fused to transcriptional activators. Further analysis determined that this appro...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="024103fc143c5a392e4ce0fc4bac971b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":87356021,"asset_id":81243231,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/87356021/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243231"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243231"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243231; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243231]").text(description); $(".js-view-count[data-work-id=81243231]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243231; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243231']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243231, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "024103fc143c5a392e4ce0fc4bac971b" } } $('.js-work-strip[data-work-id=81243231]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243231,"title":"Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing","translated_title":"","metadata":{"abstract":"Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (, , ) could be simultaneously and stably deleted in naive, memory, effector, or regulatory T cell (Treg) subsets at very high efficiency. Additionally, nuclease-deficient Cas9, associated with a transcriptional activator or repressor, can downregulate or increase expression of genes in T cells. For example, expression of glycoprotein A repetitions predominant (GARP), a gene that is normally and exclusively expressed on activated Tregs, could be induced on non-Treg effector T cells by nuclease-deficient Cas9 fused to transcriptional activators. Further analysis determined that this appro...","publication_date":{"day":18,"month":1,"year":2018,"errors":{}},"publication_name":"Journal of immunology (Baltimore, Md. : 1950)"},"translated_abstract":"Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (, , ) could be simultaneously and stably deleted in naive, memory, effector, or regulatory T cell (Treg) subsets at very high efficiency. Additionally, nuclease-deficient Cas9, associated with a transcriptional activator or repressor, can downregulate or increase expression of genes in T cells. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243219"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243219/HIV_Infected_Children_Have_Lower_Frequencies_of_CD8_Mucosal_Associated_Invariant_T_MAIT_Cells_that_Correlate_with_Innate_Th17_and_Th22_Cell_Subsets"><img alt="Research paper thumbnail of HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets" class="work-thumbnail" src="https://attachments.academia-assets.com/87356013/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243219/HIV_Infected_Children_Have_Lower_Frequencies_of_CD8_Mucosal_Associated_Invariant_T_MAIT_Cells_that_Correlate_with_Innate_Th17_and_Th22_Cell_Subsets">HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets</a></div><div class="wp-workCard_item"><span>PloS one</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocompatibi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocompatibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and -positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity, MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. In this study we evaluated the frequency of MAIT cells in perinatally HIV-infected children, their response to antiretroviral treatment and their associations with HIV clinical status and related innate and adaptive ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cafaa9983471b710b5192c89fa1324ef" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":87356013,"asset_id":81243219,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/87356013/download_file?st=MTczMjgwNTA5OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243219"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243219"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243219; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243219]").text(description); $(".js-view-count[data-work-id=81243219]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243219; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243219']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243219, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cafaa9983471b710b5192c89fa1324ef" } } $('.js-work-strip[data-work-id=81243219]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243219,"title":"HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets","translated_title":"","metadata":{"abstract":"Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocompatibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and -positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity, MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. In this study we evaluated the frequency of MAIT cells in perinatally HIV-infected children, their response to antiretroviral treatment and their associations with HIV clinical status and related innate and adaptive ...","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"PloS one"},"translated_abstract":"Mucosal-associated invariant T cells (MAIT) are innate T cells restricted by major histocompatibility related molecule 1 (MR1) presenting riboflavin metabolite ligands derived from microbes. Specificity to riboflavin metabolites confers MAIT cells a broad array of host-protective activity against gram-negative and -positive bacteria, mycobacteria, and fungal pathogens. MAIT cells are present at low levels in the peripheral blood of neonates and gradually expand to relatively abundant levels during childhood. Despite no anti-viral activity, MAIT cells are depleted early and irreversibly in HIV infected adults. Such loss or impaired expansion of MAIT cells in HIV-positive children may render them more susceptible to common childhood illnesses and opportunistic infections. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243156"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243156/BioGuide_Biological_System_Design_Tool"><img alt="Research paper thumbnail of BioGuide: Biological System Design Tool" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243156/BioGuide_Biological_System_Design_Tool">BioGuide: Biological System Design Tool</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">As the field of Synthetic Biology is on the rise, iGEM is growing up very fast and the number of ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">As the field of Synthetic Biology is on the rise, iGEM is growing up very fast and the number of parts in the parts registry is increasing with the addition of more complex parts each day. Finding a part according to desired purposes is becoming more and more difficult. After facing some difficulty while running our algorithms on the parts registry and looking the parts for desired purposes, the need for more effective standardization of parts become more apparent. We have decided to investigate the information on parts in iGEM’s 2010 distribution and reorganize the information on the parts registry forms according to the needs of our algorithm and the needs of the other parts registry users. Furthermore, to provide alternative pathways to construct the most reliable and functional Biobrick devices we have used Graph Theoretic modeling to be able to refine and traverse the parts according to their input and output information by using BioGuide application</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243156"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243156"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243156; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243156]").text(description); $(".js-view-count[data-work-id=81243156]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243156; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243156']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243156, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=81243156]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243156,"title":"BioGuide: Biological System Design Tool","translated_title":"","metadata":{"abstract":"As the field of Synthetic Biology is on the rise, iGEM is growing up very fast and the number of parts in the parts registry is increasing with the addition of more complex parts each day. Finding a part according to desired purposes is becoming more and more difficult. After facing some difficulty while running our algorithms on the parts registry and looking the parts for desired purposes, the need for more effective standardization of parts become more apparent. We have decided to investigate the information on parts in iGEM’s 2010 distribution and reorganize the information on the parts registry forms according to the needs of our algorithm and the needs of the other parts registry users. Furthermore, to provide alternative pathways to construct the most reliable and functional Biobrick devices we have used Graph Theoretic modeling to be able to refine and traverse the parts according to their input and output information by using BioGuide application"},"translated_abstract":"As the field of Synthetic Biology is on the rise, iGEM is growing up very fast and the number of parts in the parts registry is increasing with the addition of more complex parts each day. Finding a part according to desired purposes is becoming more and more difficult. After facing some difficulty while running our algorithms on the parts registry and looking the parts for desired purposes, the need for more effective standardization of parts become more apparent. We have decided to investigate the information on parts in iGEM’s 2010 distribution and reorganize the information on the parts registry forms according to the needs of our algorithm and the needs of the other parts registry users. Furthermore, to provide alternative pathways to construct the most reliable and functional Biobrick devices we have used Graph Theoretic modeling to be able to refine and traverse the parts according to their input and output information by using BioGuide application","internal_url":"https://www.academia.edu/81243156/BioGuide_Biological_System_Design_Tool","translated_internal_url":"","created_at":"2022-06-11T09:27:09.070-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"BioGuide_Biological_System_Design_Tool","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan"},"attachments":[],"research_interests":[{"id":3629,"name":"Synthetic Biology","url":"https://www.academia.edu/Documents/in/Synthetic_Biology"},{"id":5026,"name":"Genetic Algorithms","url":"https://www.academia.edu/Documents/in/Genetic_Algorithms"},{"id":1317092,"name":"IGEM","url":"https://www.academia.edu/Documents/in/IGEM"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="81243146"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/81243146/CRISPR_Of_Things_Applications_and_Challenges_of_the_Most_Popular_Gene_Editing_Tool_in_the_Fields_of_Health_Agriculture_and_Environment"><img alt="Research paper thumbnail of CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/81243146/CRISPR_Of_Things_Applications_and_Challenges_of_the_Most_Popular_Gene_Editing_Tool_in_the_Fields_of_Health_Agriculture_and_Environment">CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment</a></div><div class="wp-workCard_item"><span>International Journal of Innovative Approaches in Science Research</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Almost all cells of any living organism contain DNA, a hereditary molecule that passes from gener...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Almost all cells of any living organism contain DNA, a hereditary molecule that passes from generation to generation during reproduction. The term &quot;genome&quot; generally refers to the total DNA sequences in an organism. The genome consists of DNA sequences called “gene”, which plays a role in the basic biological processes involved in many phenotypic and genotypic characteristics, such as performing cellular functions, controlling numbers and species, regulating energy production, metabolism, and combating diseases. Gene editing is the process of pre-designing and modifying a particular DNA sequence in a targeted gene. The most widely used technique is CRISPR-Cas technology. For this purpose, the DNA helix is cut at a certain point, to form a double-strand break (DSB), and naturally existing cellular repair mechanisms repair the DSB. Modes of the repair mechanisms may affect the gene function. When DSB is formed, gene editing techniques can be applied to remove, insert, or replace a newly modified sequence using a synthetic donor template DNA. In developed and developing countries, CRISPR-Cas studies in addition to research and development studies are rapidly increasing. In addition to increasing population, changing weather conditions, declining farmland, increasing biotic and abiotic stresses are other important barriers to agricultural production, food, and feed supply. In this report, CRISPR-Cas applications are introduced in detail from the studies that carried out gene modifications in the fields of health, animals, plants, microorganisms, and food supply. Besides, these technologies and applications have been examined in terms of world biosafety legislation and the scientific risk assessment of the products developed using the CRISPR-Cas technique.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="81243146"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="81243146"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 81243146; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=81243146]").text(description); $(".js-view-count[data-work-id=81243146]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 81243146; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='81243146']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 81243146, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=81243146]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":81243146,"title":"CRISPR-Of-Things: Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment","translated_title":"","metadata":{"abstract":"Almost all cells of any living organism contain DNA, a hereditary molecule that passes from generation to generation during reproduction. The term \u0026quot;genome\u0026quot; generally refers to the total DNA sequences in an organism. The genome consists of DNA sequences called “gene”, which plays a role in the basic biological processes involved in many phenotypic and genotypic characteristics, such as performing cellular functions, controlling numbers and species, regulating energy production, metabolism, and combating diseases. Gene editing is the process of pre-designing and modifying a particular DNA sequence in a targeted gene. The most widely used technique is CRISPR-Cas technology. For this purpose, the DNA helix is cut at a certain point, to form a double-strand break (DSB), and naturally existing cellular repair mechanisms repair the DSB. Modes of the repair mechanisms may affect the gene function. When DSB is formed, gene editing techniques can be applied to remove, insert, or replace a newly modified sequence using a synthetic donor template DNA. In developed and developing countries, CRISPR-Cas studies in addition to research and development studies are rapidly increasing. In addition to increasing population, changing weather conditions, declining farmland, increasing biotic and abiotic stresses are other important barriers to agricultural production, food, and feed supply. In this report, CRISPR-Cas applications are introduced in detail from the studies that carried out gene modifications in the fields of health, animals, plants, microorganisms, and food supply. Besides, these technologies and applications have been examined in terms of world biosafety legislation and the scientific risk assessment of the products developed using the CRISPR-Cas technique.","publisher":"Pen Academic Publishing","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"International Journal of Innovative Approaches in Science Research"},"translated_abstract":"Almost all cells of any living organism contain DNA, a hereditary molecule that passes from generation to generation during reproduction. The term \u0026quot;genome\u0026quot; generally refers to the total DNA sequences in an organism. The genome consists of DNA sequences called “gene”, which plays a role in the basic biological processes involved in many phenotypic and genotypic characteristics, such as performing cellular functions, controlling numbers and species, regulating energy production, metabolism, and combating diseases. Gene editing is the process of pre-designing and modifying a particular DNA sequence in a targeted gene. The most widely used technique is CRISPR-Cas technology. For this purpose, the DNA helix is cut at a certain point, to form a double-strand break (DSB), and naturally existing cellular repair mechanisms repair the DSB. Modes of the repair mechanisms may affect the gene function. When DSB is formed, gene editing techniques can be applied to remove, insert, or replace a newly modified sequence using a synthetic donor template DNA. In developed and developing countries, CRISPR-Cas studies in addition to research and development studies are rapidly increasing. In addition to increasing population, changing weather conditions, declining farmland, increasing biotic and abiotic stresses are other important barriers to agricultural production, food, and feed supply. In this report, CRISPR-Cas applications are introduced in detail from the studies that carried out gene modifications in the fields of health, animals, plants, microorganisms, and food supply. Besides, these technologies and applications have been examined in terms of world biosafety legislation and the scientific risk assessment of the products developed using the CRISPR-Cas technique.","internal_url":"https://www.academia.edu/81243146/CRISPR_Of_Things_Applications_and_Challenges_of_the_Most_Popular_Gene_Editing_Tool_in_the_Fields_of_Health_Agriculture_and_Environment","translated_internal_url":"","created_at":"2022-06-11T09:26:39.198-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":20976874,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"CRISPR_Of_Things_Applications_and_Challenges_of_the_Most_Popular_Gene_Editing_Tool_in_the_Fields_of_Health_Agriculture_and_Environment","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":20976874,"first_name":"Cihan","middle_initials":null,"last_name":"Tastan","page_name":"CihanTastan","domain_name":"uskudar","created_at":"2014-11-07T08:02:06.675-08:00","display_name":"Cihan Tastan","url":"https://uskudar.academia.edu/CihanTastan","email":"M0p5eUtzWnV4YnJlM0xoVStBRFBOVHRrTWlSeGpzRThDdUh4bjhKSkxxMHM3UXI0ditjb3dMN3hzVWpWaDNVdC0tWnFEZXYyZlFwdkFNVEwzTHQ2THVkQT09--7c49a4e4c583cb5e4d87cf39cb696002de392acc"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":70125,"name":"CRISPR","url":"https://www.academia.edu/Documents/in/CRISPR"},{"id":1253071,"name":"Crispr-cas System","url":"https://www.academia.edu/Documents/in/Crispr-cas_System"},{"id":1261518,"name":"Genome Editing","url":"https://www.academia.edu/Documents/in/Genome_Editing"}],"urls":[]}, dispatcherData: dispatcherData }); 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