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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="heading section"><h3> GlobalFiler STR kits offer superior discriminatory power for international data sharing </h3></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div class="text parbase section"><p>Around the world, forensic DNA labs are being asked to do more with less. This is why the Applied Biosystems GlobalFiler IQC, GlobalFiler, and GlobalFiler Express PCR amplification kits combine reduced amplification time with exceptional discrimination power, even on the most challenging sample types.</p></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p>As global forensic DNA databases rapidly expand, so does the need for more discriminating STR multiplexes that can maximize loci overlap. That’s why GlobalFiler kits incorporate the most commonly used loci—all in a single multiplex, 6-dye configuration kit. GlobalFiler kits contain all markers recommended for inclusion by the CODIS Core Loci Working Group and those markers commonly used in Europe. The multiplex selection of markers reduces the risk of adventitious matches while enabling more effective cross-border data sharing. And the kits are backed by training, service, and support from Thermo Fisher Scientific.</p></div><div class="text parbase section"><p><a href="https://link.springer.com/article/10.1007/s00414-018-1817-5" target="_blank"><span class="btn btn-featured">Read the paper</span></a>    <a href="/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler.html#order" target="_blank"></a><b><a href="/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler.html?cid=fl-globalfiler#order">Ordering information for GlobalFiler kits ›</a></b></p></div><div class="heading section"><h2><br>Key benefits </h2></div><div class="accordion section"><div class="accordion" id="98aa4145bee84ff1992fc901e8f87de1" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#98aa4145bee84ff1992fc901e8f87de1" href="#qualitycontrolsystem"><span class="caret"></span> Internal Quality Control System </a></div><div class="accordion-body collapse " id="qualitycontrolsystem"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>The GlobalFiler kit is also available with an Internal Quality Control system, or IQC, as part of a fully integrated and validated forensic workflow. The IQC system consists of two synthetic sequences with specific primers for each of the targets (IQCS and IQCL) and provides positive confirmation of sample amplification, but also indicates adverse conditions with compromised amplification, such as the presence of PCR inhibitors. The IQC system provides additional confidence in genotyping results and can help forensic scientists distinguish, for example, between inhibited and degraded DNA samples.</p></div><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_418citemParsysimage_e865"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_418c/itemParsys/image_e865/foregroundimg.img.full.high.png/1686642720912.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_418c/itemParsys/image_e865/foregroundimg.img.full.high.png/1686642720912.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_418c/itemParsys/image_e865/foregroundimg.img.320.high.png/1686642720912.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_418c/itemParsys/image_e865/foregroundimg.img.480.high.png/1686642720912.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_418c/itemParsys/image_e865/foregroundimg.img.320.medium.png/1686642720912.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_418c/itemParsys/image_e865/foregroundimg.img.620.high.png/1686642720912.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_418c/itemParsys/image_e865/foregroundimg.img.320.low.png/1686642720912.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_418c/itemParsys/image_e865/foregroundimg.img.480.medium.png/1686642720912.png" alt='GFpage' title='GFpage'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_418citemParsysimage_e865 img{ width:100%; max-width: 366px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_418citemParsysimage_e865 .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_418citemParsysimage_e865 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#98aa4145bee84ff1992fc901e8f87de1" href="#Forensic"><span class="caret"></span> Forensic DNA grade manufacturing </a></div><div class="accordion-body collapse " id="Forensic"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div container-id="cq-colctrl-lt1" comp-id="A1"><div class="text parbase section"><p>GlobalFiler kits are manufactured at our location in Warrington, UK, at a facility that meets the guidelines for ISO 18385 certification. We have made significant investments across all aspects of production to minimize human DNA contamination. The result: powerful forensic DNA-grade solutions that enable you to provide answers with certainty and confidence.</p></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"><div container-id="cq-colctrl-lt1" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_de39itemParsysimage_b037"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_de39/itemParsys/image_b037/foregroundimg.img.full.high.jpg/1686642721018.jpg' media="(min-width: 1px)"><img 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alt='iso-badge' title='iso-badge'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_de39itemParsysimage_b037 img{ width:100%; max-width: 150px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_de39itemParsysimage_b037 .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_de39itemParsysimage_b037 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#98aa4145bee84ff1992fc901e8f87de1" href="#faster"><span class="caret"></span> Faster results </a></div><div class="accordion-body collapse " id="faster"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h3> Turn samples into answers in less than 2 hours </h3></div><div class="text parbase section"><p>This breakthrough chemistry enables up to 5 times faster amplification time than previous-generation kits:</p><ul><li>GlobalFiler Express Kit for single source samples: ~40 minute amplification</li><li>GlobalFiler Kit for casework samples: ~80 minute amplification</li></ul></div><div 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alt='hid-images.par.84500.image.678.346.1' title='hid-images.par.84500.image.678.346.1'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_9087itemParsysimage_ae29 img{ width:100%; max-width: 678px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_9087itemParsysimage_ae29 .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_9087itemParsysimage_ae29 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="text parbase section"><p>Typical workflow for up to 48 samples using Applied Biosystems direct PCR amplification kits, genetic analyzers and expert system analysis software.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#98aa4145bee84ff1992fc901e8f87de1" href="#greater"><span class="caret"></span> Greater discrimation </a></div><div class="accordion-body collapse " id="greater"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h3> Up to 9 orders of magnitude more discrimination power </h3></div><div class="text parbase section"><ul><li>24 loci multiplex enables up to 9 orders of magnitude more discrimination power than previous-generation kits</li><li>Includes 10 powerful mini-STR loci for increased information recovery from heavily degraded samples</li><li>Enhanced buffer system enables superior performance on inhibited samples</li><li>Improved sensitivity and flexibility to add up to 15 μL of sample input volume enables increased allele recovery from low level samples</li></ul></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_1917itemParsysimage_3145"><picture><source 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alt='COL110245-RUSH-GFIQC-launch-Broch-Global-GRAPH' title='COL110245-RUSH-GFIQC-launch-Broch-Global-GRAPH'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_1917itemParsysimage_3145 img{ width:100%; max-width: 340px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_1917itemParsysimage_3145 .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_1917itemParsysimage_3145 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><p><small><span class="muted">The GlobalFiler Kit multiplex configuration includes all 24 loci with only 1 locus partially exceeding 400 base pairs. 10 mini-STR loci lie completely below 220 base pairs for enhanced performance on degraded samples, and all gender-specific markers are located in the green VIC channel for convenience of interpretation. The IQCS and IQCL markers are only present in GlobalFiler IQC kit.</span></small></p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_1917itemParsysimage_de8a"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_1917/itemParsys/image_de8a/foregroundimg.img.full.high.gif/1686642721331.gif' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_1917/itemParsys/image_de8a/foregroundimg.img.full.high.gif/1686642721331.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_1917/itemParsys/image_de8a/foregroundimg.img.320.high.gif/1686642721331.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_1917/itemParsys/image_de8a/foregroundimg.img.480.high.gif/1686642721331.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_1917/itemParsys/image_de8a/foregroundimg.img.320.medium.gif/1686642721331.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_1917/itemParsys/image_de8a/foregroundimg.img.620.high.gif/1686642721331.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_1917/itemParsys/image_de8a/foregroundimg.img.320.low.gif/1686642721331.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_1917/itemParsys/image_de8a/foregroundimg.img.480.medium.gif/1686642721331.gif" alt='hid-images.par.49999.image.322.180.1.' title='hid-images.par.49999.image.322.180.1.'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_1917itemParsysimage_de8a img{ width:100%; max-width: 322px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_1917itemParsysimage_de8a .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_1917itemParsysimage_de8a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><p><small><span class="muted">The GlobalFiler Kit allelic ladder includes 343 alleles featuring expanded marker ranges for many loci while maintaining interlocus spacing. The expanded panel and virtual bins (589 total alleles) minimize OL allele calls and facilitate more accurate and efficient automated genotyping.</span></small></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#98aa4145bee84ff1992fc901e8f87de1" href="#global"><span class="caret"></span> Global standards </a></div><div class="accordion-body collapse " id="global"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>As global forensic DNA databases rapidly expand, so does the need for more discriminating STR multiplexes that can maximize loci overlap. That’s why GlobalFiler kits incorporate as many commonly used loci as possible—all in a single multiplex.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div container-id="cq-colctrl-lt1" comp-id="A1"><div class="text parbase section"><ul><li>GlobalFiler kits contain all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe</li><li>Reduces the risk of adventitious matches while enabling more effective cross-border data sharing</li><li>The GlobalFiler and GlobalFiler Express kits are  approved by the FBI’s National DNA Index System (NDIS) Board for use by laboratories generating offender DNA profiles for inclusion in the U.S. National NDIS database</li></ul></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"><div container-id="cq-colctrl-lt1" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_6a0aitemParsysimage_f95f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_f95f/foregroundimg.img.full.high.png/1686642721540.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_f95f/foregroundimg.img.full.high.png/1686642721540.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_f95f/foregroundimg.img.320.high.png/1686642721540.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_f95f/foregroundimg.img.480.high.png/1686642721540.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_f95f/foregroundimg.img.320.medium.png/1686642721540.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_f95f/foregroundimg.img.620.high.png/1686642721540.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_f95f/foregroundimg.img.320.low.png/1686642721540.png,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_f95f/foregroundimg.img.480.medium.png/1686642721540.png" alt='hid-images.par.4141.image.534.328.1.' title='hid-images.par.4141.image.534.328.1.'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_6a0aitemParsysimage_f95f img{ width:100%; max-width: 160px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_6a0aitemParsysimage_f95f .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_6a0aitemParsysimage_f95f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><p><i>“Improving quality control processes and performance checks is a continuous challenge in forensics, more particularly for rapid, high-throughput, and reliable generation of DNA profiles from casework samples. The Internal Quality Control (IQC) system combined into GlobalFiler kits generates a powerful and controlled STR multiplex PCR assay for better and faster decision making and data interpretation.”</i></p><p>—<b>Dr Sylvain Hubac </b><br> Research and innovation manager<br> High throughput DNA unit for casework samples<br> Forensic Science laboratory of the French Gendarmerie, France</p></div></div></div></div></div></div><div class="heading section"><h3><a href="http://www.fsigenetics.com/article/S1872-4973(14)00138-0/fulltext">Improving DNA data exchange: Validation studies on a single 6 dye STR kit with 24 loci</a></h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_6a0aitemParsysimage_9032"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_9032/foregroundimg.img.full.high.jpg/1686642721640.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_9032/foregroundimg.img.full.high.jpg/1686642721640.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_9032/foregroundimg.img.320.high.jpg/1686642721640.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_9032/foregroundimg.img.480.high.jpg/1686642721640.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_9032/foregroundimg.img.320.medium.jpg/1686642721640.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_9032/foregroundimg.img.620.high.jpg/1686642721640.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_9032/foregroundimg.img.320.low.jpg/1686642721640.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_6a0a/itemParsys/image_9032/foregroundimg.img.480.medium.jpg/1686642721640.jpg" alt='improving-dna-data' title='improving-dna-data'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_6a0aitemParsysimage_9032 img{ width:100%; max-width: 195px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_6a0aitemParsysimage_9032 .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_6a0aitemParsysimage_9032 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><p><b>Pablo Martín, Lourdes Fernández de Simón, Gracia Luque, María José Farfán, Antonio Alonso</b></p><p>DOI:<a href="http://dx.doi.org/10.1016/j.fsigen.2014.07.002" target="_blank"> http://www.fsigenetics.com/article/S1872-4973(14)00138-0/fulltext</a><u><br></u>p68–78<br> Published online: July 15, 2014<br> For Forensic or Paternity Use Only.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#98aa4145bee84ff1992fc901e8f87de1" href="#6dye"><span class="caret"></span> 6-dye chemistry </a></div><div class="accordion-body collapse " id="6dye"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>For the past 16 years, Thermo Fisher Scientific has led the way in the evolution of STR technology. And today, GlobalFiler kits utilize breakthrough 6-dye chemistry to enable optimal performance and unprecedented discrimination.</p></div><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_db69itemParsysimage_4b1d"><a class="image-enlarge-link" data-lightbox="image_4b1d" href="/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/_jcr_content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/backgroundimg.img.jpg/1686642721818.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.320.low.jpg/1686642721791.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.320.medium.jpg/1686642721791.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.480.medium.jpg/1686642721791.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.620.high.jpg/1686642721791.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.full.high.jpg/1686642721791.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.320.high.jpg/1686642721791.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.480.high.jpg/1686642721791.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.320.medium.jpg/1686642721791.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.620.high.jpg/1686642721791.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.320.low.jpg/1686642721791.jpg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_4b1d/foregroundimg.img.480.medium.jpg/1686642721791.jpg" alt='hid-images.par.94135.image.711.287.1' title='hid-images.par.94135.image.711.287.1'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_db69itemParsysimage_4b1d img{ width:100%; max-width: 860px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_db69itemParsysimage_4b1d .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_db69itemParsysimage_4b1d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><div align="right"><FONT COLOR="#007ED5">&CirclePlus; Enlarge</FONT></div></small></div></div></div><div class="text parbase section"><ul><li><b>Minimizes the number of loci</b> that are<b> </b>>400 bp, which decreases drop-outs with degraded and challenging samples</li><li><b>Increases the number of  miniSTRs</b>—the GlobalFiler Kits include 10 STRs completely under 220 bp</li><li><b>Allows for better spacing</b> between adjacent markers and expansion of  marker ranges to minimize off-ladder allele calls</li></ul></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_db69itemParsysimage_a1f2"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_a1f2/foregroundimg.img.full.high.gif/1686642721856.gif' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_a1f2/foregroundimg.img.full.high.gif/1686642721856.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_a1f2/foregroundimg.img.320.high.gif/1686642721856.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_a1f2/foregroundimg.img.480.high.gif/1686642721856.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_a1f2/foregroundimg.img.320.medium.gif/1686642721856.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_a1f2/foregroundimg.img.620.high.gif/1686642721856.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_a1f2/foregroundimg.img.320.low.gif/1686642721856.gif,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/accordion_a40c/itemspar/accordionitem_db69/itemParsys/image_a1f2/foregroundimg.img.480.medium.gif/1686642721856.gif" alt='data.par.98299.image..1' title='data.par.98299.image..1'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_db69itemParsysimage_a1f2 img{ width:100%; 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max-width: 417px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_db69itemParsysimage_e95 .image-contain" + "," + "#cq-image-jsp-"+"3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysaccordion_a40citemsparaccordionitem_db69itemParsysimage_e95 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p>Using a 6-dye configuration enables optimal performance, efficiency, data recovery and genotyping accuracy.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#98aa4145bee84ff1992fc901e8f87de1').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#98aa4145bee84ff1992fc901e8f87de1').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="text parbase section"><h2>Key features of the GlobalFiler kits</h2><div class="table-component-wrapper"><table border="1" cellspacing="0" cellpadding="0" class="rte-table" id="table-1633911276"><tbody><tr><td width="196" valign="top"> </td><td width="127" valign="top"><b>GlobalFiler kit</b></td><td width="134" valign="top"><b>GlobalFiler IQC kit</b></td><td width="130" valign="top"><b>GlobalFiler Express kit</b></td></tr><tr><td width="196" valign="top"><b>High discrimination power</b></td><td width="392" colspan="3" valign="top">24-marker multiplex assay including 3 gender markers and the highly discriminatory SE33 locus</td></tr><tr><td width="196" valign="top"><b>Number of dyes</b></td><td width="392" colspan="3" valign="top">6</td></tr><tr><td width="196" valign="top"><b>Mini-STR (<220 bp)</b></td><td width="392" colspan="3" valign="top">10</td></tr><tr><td width="196" valign="top"><b>Gender markers</b></td><td width="392" colspan="3" valign="top">Y-indel, amelogenin, and DYS391</td></tr><tr><td width="196" valign="top"><b>IQC markers</b></td><td width="127" valign="top">No</td><td width="134" valign="top">Yes: for distinguishing inhibited and degraded sample; positive control for PCR amplification</td><td width="130" valign="top">No</td></tr><tr><td width="196" valign="top"><b>Identical primer sequences</b></td><td width="127" valign="top">✓</td><td width="134" valign="top">✓ (except for the IQC)</td><td width="130" valign="top">✓</td></tr><tr><td width="196" rowspan="4" valign="top"><b>Probability of identity (PI) value</b></td><td width="392" colspan="3" valign="top">African American: 6.8 x 10<sup>-27</sup></td></tr><tr><td width="392" colspan="3" valign="top">US Caucasian: 3.71 x 10<sup>-26</sup></td></tr><tr><td width="392" colspan="3" valign="top">US Hispanic: 3.09 x 10<sup>-26</sup></td></tr><tr><td width="392" colspan="3" valign="top">Asian: 3.24 x 10<sup>-24</sup></td></tr><tr><td width="196" valign="top"><b>Required ESS markers</b></td><td width="127" valign="top">✓</td><td width="134" valign="top">✓</td><td width="130" valign="top">✓</td></tr><tr><td width="196" valign="top"><b>Required CODIS markers</b></td><td width="127" valign="top">✓</td><td width="134" valign="top">✓</td><td width="130" valign="top">✓</td></tr><tr><td width="196" valign="top"><b>NDIS* approved</b></td><td width="127" valign="top">✓</td><td width="134" valign="top">In progress</td><td width="130" valign="top">✓</td></tr><tr><td width="196" valign="top"><b>DNA</b><b>input</b></td><td width="261" colspan="2" valign="top">15 μL/1 ng target</td><td width="130" valign="top">Treated or untrested paper: 1.2 punch Swab: 3 μL (of 400 L)<br> Applied Biosystem Prep-n-Go Buffer</td></tr><tr><td width="196" valign="top"><b>Final PCR volume</b></td><td width="261" colspan="2" valign="top">25 μL</td><td width="130" valign="top">15 μL</td></tr><tr><td width="196" valign="top"><b>Technical note supporting <br> direct amplification</b></td><td width="127" valign="top">✓</td><td width="134" valign="top">No</td><td width="130" valign="top">✓</td></tr><tr><td width="196" valign="top"><b>Supported sample types</b></td><td width="127" valign="top" colspan="2">Optimized chemistry for challenging samples types: touched, inhibited, or degraded samples</td><td width="130" valign="top">Verified with multiple sample collection devices such as treated paper, untreated paper, and swabs; designed to work with the most commonly used substrates</td></tr></tbody></table></div></div><div class="heading section"><h2><br>Featured video </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="brightcoveplayer section"><div data-video="e4fcaba4a91a"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-e4fcaba4a91a', 10); </script><div id="e4fcaba4a91a-Modal" class="modal modal-video" tabindex="-1" role="dialog" aria-labelledby="e4fcaba4a91a-Modal-Label" aria-hidden="true" style="display: none;"><div class="modal-header"><button type="button" class="close" data-dismiss="modal" aria-hidden="true">x</button></div><div data-video-modal-body="e4fcaba4a91a" class="modal-body"></div></div><script> $(document).ready(function(){ $('#e4fcaba4a91a').bind('click', function () { $.ajax({url: '/content/lifetech/ipac/en-sg/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/brightcoveplayer_707.ajax.html?cached=false', data: { account: '3663210762001', videoPlayer: '5026905809001', playerPath: '/etc/brightcovetools/cbd-video-players/cbd-gwi-test-player' }, success: function(result) { $('[data-video-modal-body*=e4fcaba4a91a]').html(result); 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", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/52dcccf8-2427-4be2-8a84-c1e93efa1e95/d23a6b23-354c-4293-9c77-5945fc5a3fc6/160x90/match/image.jpg"], "uploadDate": "2016-07-08T00:11:14.130Z", "duration": "PT7M11.915S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/5026905809001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=5026905809001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=5026905809001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="heading section"><div class="h3"> High discrimination power of GlobalFiler kit impresses Dr. Antonio Alonso </div></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div class="text parbase section"><p>Dr. Antonio Alonso discusses how forensic laboratories can make the transition to 6-dye chemistries in order to gain workflow efficiency and reduce operating costs.</p></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2> Featured blog post and study </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysimage_6eeb"><a href="http://acceleratingscience.com/behindthebench/flexibility-of-6-dye-chemistries-in-recovering-maximum-information"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/image_6eeb.img.full.high.jpeg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/image_6eeb.img.full.high.jpeg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/image_6eeb.img.320.high.jpeg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/image_6eeb.img.480.high.jpeg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/image_6eeb.img.320.medium.jpeg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/image_6eeb.img.620.high.jpeg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/image_6eeb.img.320.low.jpeg,https://www.thermofisher.com/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/image_6eeb.img.480.medium.jpeg" alt='Bone samples' title='Bone samples'></picture><style> #cq-image-jsp-3d5cb54c-de5d-4121-ab57-82b56fe88fd7MainParsysimage_6eeb img{ width:100%; max-width: 100%; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><p style="font-size: 15px;"><b>Recovering maximum information from degraded samples</b></p><p>In order to enhance information recovery from challenging bone samples, a recent degradation study was conducted by the University of North Texas Health Science Center, Department of Forensic and Investigative Genetics, US.</p><p><a class="btn btn-primary" href="http://acceleratingscience.com/behindthebench/flexibility-of-6-dye-chemistries-in-recovering-maximum-information" target="_blank">Read more</a></p><ul class="the-icons clearfix"><li><span class="icon icon-pdf"></span><a href="/content/dam/LifeTech/Documents/PDFs/Degradation-Study-STRForce-PhaseI.pdf" target="_blank">Performance results</a></li></ul></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2> Featured documents </h2></div><div class="text parbase section"><ul class="the-icons clearfix"><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/GSD/Application-Notes/improve-interpretation-results-bone-samples-application-note.pdf" target="_blank">Application note: Improve interpretation results from bone samples with GlobalFilerIQC Kit</a></li><li><span class="icon-lt-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/GSD/Application-Notes/get-more-answers-from-challenging-bone-samples-app-note.pdf" target="_blank">Application Note: Get more answers from challenging bone samples</a></li><li><span class="icon-lt-pdf"></span><a href="/content/dam/LifeTech/Documents/PDFs/Technical-Bulletin-GlobalFiler-3130xL-decreased-cycle.pdf" target="_blank">Technical Bulletin: GlobalFiler 3130xL 28-cycle protocol</a></li><li><span class="icon-lt-pdf"></span><a href="/content/dam/LifeTech/Documents/PDFs/Technical-Note-GlobalFiler-direct-amp.pdf" target="_blank">Technical Note: GlobalFiler Direct kit</a></li><li><span class="icon-lt-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/GSD/brochures/globalfiler-str-brochure.pdf">Brochure: Globalfiler kits</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/globalfiler-swgdam-validation-certificate.pdf" target="_blank"><span class="icon-lt-pdf"></span>Validation Certificate</a></li><li><a href="http://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/globalfileriqc-swgdam-validation-certificate.pdf" target="_blank"><span class="icon-lt-pdf"></span>Validation certificate: GlobalFiler IQC kit</a></li></ul></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Need help bringing products online? </h3></div><div class="text parbase section"><p>Look no further than our<a href="/sg/en/home/industrial/forensics/human-identification/human-identification-professional-services.html" target="_blank"> Human Identification Professional Services (HPS) <span class="icon icon-outbound"> </span></a>team. Since 2007, we have completed over 400 successful validation projects worldwide with a team of more than 20 technical support specialists, each averaging 8 years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.</p><p><a href="/sg/en/home/industrial/forensics/human-identification/human-identification-professional-services.html"><span class="btn btn-primary">Learn more</span></a></p></div></div></div></div></div></div><div class="heading section"><div class="h2"><br>Resources & FAQs </div></div><div class="accordion section"><div class="accordion" id="d3cb9277eda44fc1b0e32a76e56cc729" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d3cb9277eda44fc1b0e32a76e56cc729" href="#presentations"><span class="caret"></span> GlobalFiler presentations </a></div><div class="accordion-body collapse " id="presentations"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><ul><li><a href="/sg/en/home/global/forms/globalfiler-fsi-genetics-article-download.html">National Institute of Toxicology & Forensic Sciences, Madrid: Globalfiler Kit Validation</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/saint-louis-globalfiler-kit-validation-presentation.pdf" target="_blank">Saint Louis County Police Crime Lab: GlobalFiler Kit Validation</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/GSD/posters/st-louis-county-police-interesting-cases-poster.pdf" target="_blank">Saint Louis County Police Crime Lab: Interesting Cases</a></li><li><a href="/content/dam/LifeTech/Documents/PDFs/kansas-city-police-crime-lab.pdf" target="_blank">Kansas City Police Crime Laboratory: Validation of GlobalFiler, Quantifiler on the 3500 GA</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/globalfiler-hps-tip-tricks-2015-presentation.pdf" target="_blank">HID Professional Services (HPS) tips and tricks for validations of the GlobalFiler™ Kit</a></li></ul></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d3cb9277eda44fc1b0e32a76e56cc729" href="#globalfilerpublications"><span class="caret"></span> GlobalFiler publications </a></div><div class="accordion-body collapse " id="globalfilerpublications"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p><a href="https://link.springer.com/article/10.1007/s00414-018-1817-5" target="_blank">Ludeman, M., et al (2018).Developmental validation of GlobalFiler™ PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples <span class="icon icon-outbound"></span></a></p><p><a href="http://www.fsigenetics.com/article/S1872-4973(17)30041-8/abstract">Taylor, D.,et al (2017). Allele frequency database for GlobalFiler™ STR loci in Australian and New Zealand populations <span class="icon icon-outbound"></span></a></p><p>Wu, L., et al (2017). Population genetic analysis of Xiamen Han population on 21 short tandem repeat loci  </p><p><a href="https://link.springer.com/article/10.1007/s13258-016-0511-6">Choi, E.-J., et al (2017). Forensic and population genetic analyses of the GlobalFiler STR loci in the Mongolian population <span class="icon icon-outbound"></span></a></p><p><a href="https://link.springer.com/article/10.1007/s00414-016-1497-y">Guerreiro, S., et al (2017). Characterization of GlobalFiler loci in Angolan and Guinean populations inhabiting Southern Portugal <span class="icon icon-outbound"></span></a></p><p><a href="https://link.springer.com/article/10.1007/s00414-016-1319-2">Zhang, H., et al (2016). Population genetic analysis of the GlobalFiler STR loci in 748 individuals from the Kazakh population of Xinjiang in northwest China <span class="icon icon-outbound"></span></a></p><p><a href="http://www.fsigenetics.com/article/S1872-4973(16)30127-2/abstract">Ristow, P.G., et al (2016). GlobalFiler<sup>®</sup> Express DNA amplification kit in South Africa: Extracting the past from the present <span class="icon icon-outbound"></span></a></p><p><a href="http://www.fsigeneticssup.com/article/S1875-1768(15)30093-7/abstract">Bogas, V., et al (2015).Testing the behavior of GlobalFiler<sup>®</sup> PCR amplification kit with degraded and/or inhibited biological samples <span class="icon icon-outbound"></span></a></p><p><a href="http://www.fsigeneticssup.com/article/S1875-1768(15)30092-5/abstract">Gouveia, N., et al (2015). Direct amplification of reference samples with GlobalFiler<sup>®</sup> PCR Amplification Kit <span class="icon icon-outbound"></span></a></p><p><a href="http://www.fsigeneticssup.com/article/S1875-1768(15)30078-0/abstract">Brito, P., et al (2015). Direct amplification of casework samples in fabrics using GlobalFiler<sup>®</sup> PCR Amplification Kit <span class="icon icon-outbound"></span></a></p><p><a href="http://www.fsigenetics.com/article/S1872-4973(14)00280-4/abstract">Vernarecci, S., et al (2015). Quantifiler® Trio Kit and forensic samples management: A matter of degradation <span class="icon icon-outbound"></span></a></p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d3cb9277eda44fc1b0e32a76e56cc729" href="#brochure"><span class="caret"></span> GlobalFiler brochure </a></div><div class="accordion-body collapse " id="brochure"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p><span class="icon icon-pdf"></span><a href="http://assets.thermofisher.com/TFS-Assets/GSD/brochures/globalfiler-str-brochure.pdf"> Brochure: GlobalFiler kits</a></p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#d3cb9277eda44fc1b0e32a76e56cc729').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#d3cb9277eda44fc1b0e32a76e56cc729').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="productlist parbase section"><div id="skulist" style="display:none">4476609,4474665,4479649,4479648,4476135,4482815,4425042, A43565</div><div id='ltajax-8048817335422025756'></div><script type="text/javascript">(function() {$('#ltajax-8048817335422025756').load('/sg/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling/globalfiler/jcr:content/MainParsys/productlist_cd68.ajax.html?skulist=');})(); </script></div><div class="text parbase section"><p style="text-align: right;"><span><a href="#top">Top</a></span></p></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="heading section"><h3> Customer support </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><p>If you have additional comments or questions, please contact the <a href="mailto:hid.info@lifetech.com">customer service team via email</a>.</p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="heading section"><h3> Technical support </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><p>If you are a current customer and need technical support, please contact the <a href="mailto:hidtechsupport@appliedbiosystems.com">global service team via email</a>, or phone us at 1-888-821-4HID (4443). Select option #1 for immediate assistance.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="text parbase section"><p class="legal-text">For Research Forensic or Paternity Use Only. Not for use in diagnostic procedures. For licensing and limited use restrictions visit <a href="/HIDlicensing">thermofisher.com/HIDlicensing</a></p></div><div class="LTRawHTML section"><style> .modal { width: 785px; } </style><div id="myModal" class="modal hide fade" tabindex="-1" role="dialog" aria-labelledby="myModalLabel" aria-hidden="true"><div class="modal-header"><button type="button" class="close" data-dismiss="modal" aria-hidden="true">×</button><h3 style="border-top:none;background:none;" id="myModalLabel">Faster results</h3></div><div class="modal-body"><div class="parsys MainParsys"><div class="text parbase section"><p><strong>Turn samples Into answers in less than 2 hours</strong></p><p>This breakthrough chemistry enables up to 5 times faster amplification time than previous-generation kits:</p><ul><li>GlobalFiler™ Express Kit for single source samples: ~40 minute amplification</li><li>GlobalFiler™ Kit for casework samples: ~80 minute amplification</li></ul></div><div class="text parbase section"><p><img width="678" height="346" alt="" id="/content/dam/LifeTech/migration/images/human-identification/hid-imagespar.84500.image " src="/content/dam/LifeTech/migration/images/human-identification/hid-images.par.84500.image.678.346.1..gif"></p><p>Typical workflow for up to 48 samples using Applied Biosystems™ direct PCR amplification kits, genetic analyzers and expert system analysis software.</p><p class="legal-text">For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.</p></div></div></div><div class="modal-footer"><button class="btn btn-secondary" data-dismiss="modal">Close</button></div></div><div id="myModal2" class="modal hide fade" tabindex="-1" role="dialog" aria-labelledby="myModalLabel" aria-hidden="true"><div class="modal-header"><button type="button" class="close" data-dismiss="modal" aria-hidden="true">×</button><h3 style="border-top:none;background:none;" id="myModalLabel">Greater discrimination</h3></div><div class="modal-body"><div class="parsys MainParsys"><div class="text parbase section"><p><strong>Up to 9 orders of magnitude more discrimination power</strong></p><ul><li> 24 loci multiplex enables up to 9 orders of magnitude more discrimination power than previous-generation kits </li><li> Includes 10 powerful mini-STR loci for increased information recovery from heavily degraded samples </li><li> Enhanced buffer system enables superior performance on inhibited samples </li><li> Improved sensitivity and flexibility to add up to 15 μL of sample input volume enables increased allele recovery from low level samples </li></ul></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="text parbase section"><p>   </p><div><img width="315" height="180" src="/content/dam/LifeTech/migration/images/human-identification/hid-images.par.93551.image.315.180.1..gif"></div><p class="caption-text"> The GlobalFiler™ Kit multiplex configuration includes all 24 loci with only 1 locus partially exceeding 400 base pairs. 10 mini-STR loci lie completely below 220 base pairs for enhanced performance on degraded samples, and all gender-specific markers are located in the green VIC® channel for convenience of interpretation. </p></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p>   </p><div><img width="322" height="180" src="/content/dam/LifeTech/migration/images/human-identification/hid-images.par.49999.image.322.180.1..gif"></div><p class="caption-text"> The GlobalFiler™ Kit allelic ladder includes 343 alleles featuring expanded marker ranges for many loci while maintaining interlocus spacing. The expanded panel and virtual bins (589 total alleles) minimize OL allele calls and facilitate more accurate and efficient automated genotyping. </p></div></div></div><div style="clear:both"></div><div class="text parbase section"><p class="legal-text"> For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures. </p></div></div><div class="modal-footer"><button class="btn btn-secondary" data-dismiss="modal">Close</button></div></div></div><div id="myModal3" class="modal hide fade" tabindex="-1" role="dialog" aria-labelledby="myModalLabel" aria-hidden="true"><div class="modal-header"><button type="button" class="close" data-dismiss="modal" aria-hidden="true">×</button><h3 style="border-top:none;background:none;" id="myModalLabel">Global standards</h3></div><div class="modal-body"><div class="parsys MainParsys"><div class="text parbase section"><p>As global forensic DNA databases rapidly expand, so does the need for more discriminating STR multiplexes that can maximize loci overlap. That’s why GlobalFiler™ Kits incorporate as many commonly used loci as possible—all in a single multiplex.</p><ul><li>Only the GlobalFiler™ Kits contain <i>all</i> markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe.</li><li>Reduces the risk of adventitious matches while enabling more effective cross-border data sharing.</li></ul><hr><blockquote class="blockquote"><i>“One of the great values of GlobalFiler™ is the intelligent design that looks to the future —enhancing discrimination power while also offering the best compatibility and concordance with the historical set of 40 million STR profiles registered in many national DNA databases around the world.“</i></blockquote><blockquote>—<b>Antonio Alonso</b><br><b></b>Facultative (DNA expert), Biology Department, National Institute of Toxicology and Forensic Sciences, Spain<br> Project Manager, IDNADEX (Improving DNA Data EXchange)</blockquote><hr><p><br></p><p></p></div><div class="text parbase section"><p><a href="/us/en/home/global/forms/globalfiler-fsi-genetics-article-download.html"><img class="img-left" src="/content/dam/LifeTech/Images/content/improving-dna-data.jpg"></a><a href="/us/en/home/global/forms/globalfiler-fsi-genetics-article-download.html">Improving DNA data exchange: Validation studies on a single 6 dye STR kit with 24 loci</a><u><br></u>Pablo Martín, Lourdes Fernández de Simón, Gracia Luque, María José Farfán, Antonio Alonso<br> DOI: <a href="http://dx.doi.org/10.1016/j.fsigen.2014.07.002" target="_blank">http://dx.doi.org/10.1016/j.fsigen.2014.07.002</a><u><br></u>p68–78<br> Published online: July 15, 2014<br> For Forensic or Paternity Use Only.</p><p><a href="/us/en/home/global/forms/globalfiler-fsi-genetics-article-download.html" class="button primary-button">Request article</a></p></div><div class="text parbase section"><div style="text-align: center;"><p><img width="534" height="328" src="/content/dam/LifeTech/migration/images/human-identification/hid-images.par.4141.image.534.328.1..gif"></p><p class="legal-text" style="text-align: left;"></p><p class="legal-text" style="text-align: left;">For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.</p></div></div><div class="text parbase section"><p><img width="740" height="400" alt="" id="/content/dam/LifeTech/migration/images/human-identification/datapar.17178.image " src="/content/dam/LifeTech/migration/images/human-identification/data.par.17178.image.740.400.1..gif"></p><p> Countries with active nationwide offender DNA database programs (as of August 2012). There are 44 countries worldwide, with over 40 million total profiles estimated to be uploaded. Note: this does not include nations that have passed databasing laws/policies, but have yet to implement them. </p><p><a class="headline" target="_blank" href="http://www.dnaresource.com/">www.dnaresource.com</a></p></div></div></div><div class="modal-footer"><button class="btn btn-secondary" data-dismiss="modal">Close</button></div></div><div id="myModal4" class="modal hide fade" tabindex="-1" role="dialog" aria-labelledby="myModalLabel" aria-hidden="true"><div class="modal-header"><button type="button" class="close" data-dismiss="modal" aria-hidden="true">×</button><h3 style="border-top:none;background:none;" id="myModalLabel">6-dye Chemistry</h3></div><div class="modal-body"><div class="parsys MainParsys"><div class="text parbase section"><p> For the past 16 years, Thermo Fisher Scientific has led the way in the evolution of STR technology. And today, GlobalFiler™ Kits <br> utilize breakthrough 6-dye chemistry to enable optimal performance and unprecedented discrimination power. </p></div><div class="text parbase section"><p class="legal-text"><img width="711" height="287" alt="" id="/content/dam/LifeTech/migration/images/human-identification/hid-imagespar.94135.image " src="/content/dam/LifeTech/migration/images/human-identification/hid-images.par.94135.image.711.287.1..gif"></p><p><strong>Why 6-dye? </strong><br> Squeezing all the additional loci into a 5-dye configuration would result in multiple tradeoffs, including: <br><br><u>Tradeoff #1</u>: Multiple loci in the high molecular weight size range (>400 bp), increasing drop out with degraded and challenging samples. <br><br><u>Tradeoff #2</u>: Several loci extending almost to 500 bp, which may cause issues with sizing and resolution, i.e. alleles labeled “OL” or failure to resolve microvariant alleles. <br><br><u>Tradeoff #3</u>: Fewer miniSTRs. The GlobalFiler™ Kits include 10 STRs completely under 220 bp! This would not be possible in a 5-dye configuration. <br><br><u>Tradeoff #4</u>: Insufficient spacing between adjacent markers; more difficult to expand marker ranges to minimize “OL” allele calls. <br><br><u>Tradeoff #5</u>: Many loci would require redesigned primer sequences, leading to less concordance with data generated using the original primer sequences and with the vast majority of profiles in worldwide databases. </p><p class="legal-text"></p><div class="figure"><table width="200" cellspacing="5" cellpadding="5"><tbody><tr><td><img width="360" height="280" src="/content/dam/LifeTech/migration/images/human-identification/data.par.98299.image..1..gif?direct=1"></td><td> </td><td><img width="360" height="280" src="/content/dam/LifeTech/migration/images/human-identification/data.par.3074.image..1..gif?direct=1"></td></tr></tbody></table></div> Using a 6-dye configuration enables optimal performance, efficiency, data recovery and genotyping accuracy <p></p><p class="legal-text"> For Research, Forensic, or Paternity Use Only. 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