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class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Susan KIDSON</h3></div><div class="js-work-strip profile--work_container" data-work-id="115467566"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/115467566/Properties_and_Functions_of_the_Vessels_of_the_Ciliary_Body"><img alt="Research paper thumbnail of Properties and Functions of the Vessels of the Ciliary Body" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/115467566/Properties_and_Functions_of_the_Vessels_of_the_Ciliary_Body">Properties and Functions of the Vessels of the Ciliary Body</a></div><div class="wp-workCard_item"><span>Encyclopedia of the Eye</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from the four recti muscle arteries) and the long posterior ciliary arteries which traverse the interior. A complex arrangement of branches and anastomoses from these cells culminates circularly arranged vessels, including the episcleral arterial circle, the intramuscular circle of the ciliary muscle, and the major arterial circle (MAC). Each ciliary process receives blood from branches off the MAC, which follow a tortuous route through three vascular territories. Venules drain through the pars plana and exit via the vortex veins. CB vessels have wide lumens and are fenestrated, allowing production of aqueous humor. The complexity, density, and redundancy of vessels ensures consistent perfusion of the anterior segment.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="115467566"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="115467566"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 115467566; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=115467566]").text(description); $(".js-view-count[data-work-id=115467566]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 115467566; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='115467566']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=115467566]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":115467566,"title":"Properties and Functions of the Vessels of the Ciliary Body","translated_title":"","metadata":{"abstract":"The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from the four recti muscle arteries) and the long posterior ciliary arteries which traverse the interior. A complex arrangement of branches and anastomoses from these cells culminates circularly arranged vessels, including the episcleral arterial circle, the intramuscular circle of the ciliary muscle, and the major arterial circle (MAC). Each ciliary process receives blood from branches off the MAC, which follow a tortuous route through three vascular territories. Venules drain through the pars plana and exit via the vortex veins. CB vessels have wide lumens and are fenestrated, allowing production of aqueous humor. The complexity, density, and redundancy of vessels ensures consistent perfusion of the anterior segment.","publisher":"Elsevier","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Encyclopedia of the Eye"},"translated_abstract":"The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from the four recti muscle arteries) and the long posterior ciliary arteries which traverse the interior. A complex arrangement of branches and anastomoses from these cells culminates circularly arranged vessels, including the episcleral arterial circle, the intramuscular circle of the ciliary muscle, and the major arterial circle (MAC). Each ciliary process receives blood from branches off the MAC, which follow a tortuous route through three vascular territories. Venules drain through the pars plana and exit via the vortex veins. CB vessels have wide lumens and are fenestrated, allowing production of aqueous humor. The complexity, density, and redundancy of vessels ensures consistent perfusion of the anterior segment.","internal_url":"https://www.academia.edu/115467566/Properties_and_Functions_of_the_Vessels_of_the_Ciliary_Body","translated_internal_url":"","created_at":"2024-02-26T15:01:25.040-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Properties_and_Functions_of_the_Vessels_of_the_Ciliary_Body","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from the four recti muscle arteries) and the long posterior ciliary arteries which traverse the interior. A complex arrangement of branches and anastomoses from these cells culminates circularly arranged vessels, including the episcleral arterial circle, the intramuscular circle of the ciliary muscle, and the major arterial circle (MAC). Each ciliary process receives blood from branches off the MAC, which follow a tortuous route through three vascular territories. Venules drain through the pars plana and exit via the vortex veins. CB vessels have wide lumens and are fenestrated, allowing production of aqueous humor. The complexity, density, and redundancy of vessels ensures consistent perfusion of the anterior segment.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":54589,"name":"Anatomy","url":"https://www.academia.edu/Documents/in/Anatomy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="115467565"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/115467565/Wholemount_imaging_reveals_abnormalities_of_the_aqueous_outflow_pathway_and_corneal_vascularity_in_Foxc1_and_Bmp4_heterozygous_mice"><img alt="Research paper thumbnail of Wholemount imaging reveals abnormalities of the aqueous outflow pathway and corneal vascularity in Foxc1 and Bmp4 heterozygous mice" class="work-thumbnail" src="https://attachments.academia-assets.com/111866192/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/115467565/Wholemount_imaging_reveals_abnormalities_of_the_aqueous_outflow_pathway_and_corneal_vascularity_in_Foxc1_and_Bmp4_heterozygous_mice">Wholemount imaging reveals abnormalities of the aqueous outflow pathway and corneal vascularity in Foxc1 and Bmp4 heterozygous mice</a></div><div class="wp-workCard_item"><span>Experimental eye research</span><span>, Jan 9, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congeni...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congenital anterior segment dysgenesis (ASD) and the development of the aqueous outflow structures throughout the limbus. The aim of this study was to advance our understanding of anterior segment abnormalities in mouse models of ASD using a 3-D imaging approach. Holistic imaging information combined with quantitative measurements were carried out on PECAM-1 stained individual components of the aqueous outflow vessels and corneal vasculature of Foxc1(+/-) on the C57BL/6Jx129 and ICR backgrounds, Bmp4(+/-) ICR mice, and wildtype mice from each background. In both wildtype and heterozygotes, singular, bifurcated and plexus forms of Schlemm&#39;s canal were noted. Of note, missing portions of the canal were seen in the heterozygous groups but not in wildtype animals. In general, we found the number of collector channels to be reduced in both heterozygotes. Lastly, we found a significant increase i...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8e17f0abf58003777964770c09151494" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":111866192,"asset_id":115467565,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/111866192/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="115467565"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="115467565"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 115467565; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=115467565]").text(description); $(".js-view-count[data-work-id=115467565]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 115467565; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='115467565']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8e17f0abf58003777964770c09151494" } } $('.js-work-strip[data-work-id=115467565]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":115467565,"title":"Wholemount imaging reveals abnormalities of the aqueous outflow pathway and corneal vascularity in Foxc1 and Bmp4 heterozygous mice","translated_title":"","metadata":{"abstract":"Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congenital anterior segment dysgenesis (ASD) and the development of the aqueous outflow structures throughout the limbus. The aim of this study was to advance our understanding of anterior segment abnormalities in mouse models of ASD using a 3-D imaging approach. Holistic imaging information combined with quantitative measurements were carried out on PECAM-1 stained individual components of the aqueous outflow vessels and corneal vasculature of Foxc1(+/-) on the C57BL/6Jx129 and ICR backgrounds, Bmp4(+/-) ICR mice, and wildtype mice from each background. In both wildtype and heterozygotes, singular, bifurcated and plexus forms of Schlemm\u0026#39;s canal were noted. Of note, missing portions of the canal were seen in the heterozygous groups but not in wildtype animals. In general, we found the number of collector channels to be reduced in both heterozygotes. Lastly, we found a significant increase i...","ai_title_tag":"Corneal and Aqueous Pathway Abnormalities in Mice","publication_date":{"day":9,"month":1,"year":2016,"errors":{}},"publication_name":"Experimental eye research"},"translated_abstract":"Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congenital anterior segment dysgenesis (ASD) and the development of the aqueous outflow structures throughout the limbus. The aim of this study was to advance our understanding of anterior segment abnormalities in mouse models of ASD using a 3-D imaging approach. Holistic imaging information combined with quantitative measurements were carried out on PECAM-1 stained individual components of the aqueous outflow vessels and corneal vasculature of Foxc1(+/-) on the C57BL/6Jx129 and ICR backgrounds, Bmp4(+/-) ICR mice, and wildtype mice from each background. In both wildtype and heterozygotes, singular, bifurcated and plexus forms of Schlemm\u0026#39;s canal were noted. Of note, missing portions of the canal were seen in the heterozygous groups but not in wildtype animals. In general, we found the number of collector channels to be reduced in both heterozygotes. Lastly, we found a significant increase i...","internal_url":"https://www.academia.edu/115467565/Wholemount_imaging_reveals_abnormalities_of_the_aqueous_outflow_pathway_and_corneal_vascularity_in_Foxc1_and_Bmp4_heterozygous_mice","translated_internal_url":"","created_at":"2024-02-26T15:01:24.779-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":111866192,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/111866192/thumbnails/1.jpg","file_name":"j.exer.2016.04.00320240226-1-lkt20n.pdf","download_url":"https://www.academia.edu/attachments/111866192/download_file","bulk_download_file_name":"Wholemount_imaging_reveals_abnormalities.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/111866192/j.exer.2016.04.00320240226-1-lkt20n-libre.pdf?1708990844=\u0026response-content-disposition=attachment%3B+filename%3DWholemount_imaging_reveals_abnormalities.pdf\u0026Expires=1742176506\u0026Signature=gMOQrCII7eiHVCNLw4wrGNFlbUlrNwpxgv09c00lfsUOtW5iOQ1h~jkVht8yyJz8r6SnPjoBpgSsIC8DHdf~fef0UnGOzWjty~0zHMNx0r3gsLrInbbQpEyXP236vZWf28Y4bWEXPTWeb~BsIviCtUmJWTbjwmqgyNCsEa~lfUbMDS~Dr4CZy129q16XCRED~genoq-T415uEwD4ARneY768v7-p8HNqGqFnpYvS3qxMH~F3lSuNdhNIvpqSG7JAhM59wbSSlVzcNH2aQ6sBaXYcggssal7FXI9rIW788UXApvUmeERBYADlrHfGWbvB5pOuDziC85kfIQ0gRPK1fA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Wholemount_imaging_reveals_abnormalities_of_the_aqueous_outflow_pathway_and_corneal_vascularity_in_Foxc1_and_Bmp4_heterozygous_mice","translated_slug":"","page_count":11,"language":"en","content_type":"Work","summary":"Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congenital anterior segment dysgenesis (ASD) and the development of the aqueous outflow structures throughout the limbus. The aim of this study was to advance our understanding of anterior segment abnormalities in mouse models of ASD using a 3-D imaging approach. Holistic imaging information combined with quantitative measurements were carried out on PECAM-1 stained individual components of the aqueous outflow vessels and corneal vasculature of Foxc1(+/-) on the C57BL/6Jx129 and ICR backgrounds, Bmp4(+/-) ICR mice, and wildtype mice from each background. In both wildtype and heterozygotes, singular, bifurcated and plexus forms of Schlemm\u0026#39;s canal were noted. Of note, missing portions of the canal were seen in the heterozygous groups but not in wildtype animals. In general, we found the number of collector channels to be reduced in both heterozygotes. Lastly, we found a significant increase i...","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":111866192,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/111866192/thumbnails/1.jpg","file_name":"j.exer.2016.04.00320240226-1-lkt20n.pdf","download_url":"https://www.academia.edu/attachments/111866192/download_file","bulk_download_file_name":"Wholemount_imaging_reveals_abnormalities.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/111866192/j.exer.2016.04.00320240226-1-lkt20n-libre.pdf?1708990844=\u0026response-content-disposition=attachment%3B+filename%3DWholemount_imaging_reveals_abnormalities.pdf\u0026Expires=1742176506\u0026Signature=gMOQrCII7eiHVCNLw4wrGNFlbUlrNwpxgv09c00lfsUOtW5iOQ1h~jkVht8yyJz8r6SnPjoBpgSsIC8DHdf~fef0UnGOzWjty~0zHMNx0r3gsLrInbbQpEyXP236vZWf28Y4bWEXPTWeb~BsIviCtUmJWTbjwmqgyNCsEa~lfUbMDS~Dr4CZy129q16XCRED~genoq-T415uEwD4ARneY768v7-p8HNqGqFnpYvS3qxMH~F3lSuNdhNIvpqSG7JAhM59wbSSlVzcNH2aQ6sBaXYcggssal7FXI9rIW788UXApvUmeERBYADlrHfGWbvB5pOuDziC85kfIQ0gRPK1fA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":227709,"name":"Outflow","url":"https://www.academia.edu/Documents/in/Outflow"},{"id":359001,"name":"Optometry and Ophthalmology","url":"https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology"},{"id":1198614,"name":"Vascularity","url":"https://www.academia.edu/Documents/in/Vascularity"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences"},{"id":3789880,"name":"Medical biochemistry and metabolomics","url":"https://www.academia.edu/Documents/in/Medical_biochemistry_and_metabolomics"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="115467563"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/115467563/Postnatal_development_of_the_eye_in_the_naked_mole_rat_Heterocephalus_glaber_"><img alt="Research paper thumbnail of Postnatal development of the eye in the naked mole rat (Heterocephalus glaber)" class="work-thumbnail" src="https://attachments.academia-assets.com/111866191/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/115467563/Postnatal_development_of_the_eye_in_the_naked_mole_rat_Heterocephalus_glaber_">Postnatal development of the eye in the naked mole rat (Heterocephalus glaber)</a></div><div class="wp-workCard_item"><span>The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology</span><span>, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be visually nonfunctional and as such is an ideal animal with which to pursue questions in evolutionary developmental biology. This report is the first in-depth study on the development and morphology of the naked mole rat eye. Using standard histological analysis and scanning and transmission electron microscopy, we describe the structural features of the eye. We further report on the morphological changes that accompany the development of this eye from neonate to adult and compare them with those that occur during mouse eye development. We observed numerous abnormalities in the shape and cellular arrangement of the structures of the anterior chamber, with notable malformations of the lens. Cell proliferation and cell death assays were conducted to investigate the possible causes of lens malformation. We found that neither of these processes appeared abnormal, indicating that they were not...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9ab3e0fe54d649d02861642d45edb995" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":111866191,"asset_id":115467563,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/111866191/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="115467563"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="115467563"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 115467563; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=115467563]").text(description); $(".js-view-count[data-work-id=115467563]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 115467563; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='115467563']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9ab3e0fe54d649d02861642d45edb995" } } $('.js-work-strip[data-work-id=115467563]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":115467563,"title":"Postnatal development of the eye in the naked mole rat (Heterocephalus glaber)","translated_title":"","metadata":{"abstract":"The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be visually nonfunctional and as such is an ideal animal with which to pursue questions in evolutionary developmental biology. This report is the first in-depth study on the development and morphology of the naked mole rat eye. Using standard histological analysis and scanning and transmission electron microscopy, we describe the structural features of the eye. We further report on the morphological changes that accompany the development of this eye from neonate to adult and compare them with those that occur during mouse eye development. We observed numerous abnormalities in the shape and cellular arrangement of the structures of the anterior chamber, with notable malformations of the lens. Cell proliferation and cell death assays were conducted to investigate the possible causes of lens malformation. We found that neither of these processes appeared abnormal, indicating that they were not...","publication_date":{"day":null,"month":null,"year":2004,"errors":{}},"publication_name":"The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology"},"translated_abstract":"The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be visually nonfunctional and as such is an ideal animal with which to pursue questions in evolutionary developmental biology. This report is the first in-depth study on the development and morphology of the naked mole rat eye. Using standard histological analysis and scanning and transmission electron microscopy, we describe the structural features of the eye. We further report on the morphological changes that accompany the development of this eye from neonate to adult and compare them with those that occur during mouse eye development. We observed numerous abnormalities in the shape and cellular arrangement of the structures of the anterior chamber, with notable malformations of the lens. Cell proliferation and cell death assays were conducted to investigate the possible causes of lens malformation. We found that neither of these processes appeared abnormal, indicating that they were not...","internal_url":"https://www.academia.edu/115467563/Postnatal_development_of_the_eye_in_the_naked_mole_rat_Heterocephalus_glaber_","translated_internal_url":"","created_at":"2024-02-26T15:01:09.111-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":111866191,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/111866191/thumbnails/1.jpg","file_name":"ar.a.2002520240226-1-ln9civ.pdf","download_url":"https://www.academia.edu/attachments/111866191/download_file","bulk_download_file_name":"Postnatal_development_of_the_eye_in_the.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/111866191/ar.a.2002520240226-1-ln9civ-libre.pdf?1708990846=\u0026response-content-disposition=attachment%3B+filename%3DPostnatal_development_of_the_eye_in_the.pdf\u0026Expires=1742176506\u0026Signature=NYz4ozeJ37jntqDn8xszXzlH4Q90IPA3p4zE1JK59qf6GSgj520J8~maeZej3fRN5UOlEP6RamrNL22Z~T3Glswv0JtY~id9tH2skMyBZYBAJcZaRLdQODe8b43m1Q9ShOgoik37zUKoohPa81bReYlL5RsTNtc~Fb9oszshgiNLYbbIlgM5UsP8Bpo51xIPox~~VCLDgHG899f0NhcojLemtl~~P1fNH3PW29~lfCl8rlf3BScTUoqlQHzqoHpbgk00DO19CKuq95auW5~43jXQi-tEBqDh5Bll6HcaUKZnc0mKA1pbQgMx1UwOlx0dJdl4HxHKxJdTwhSfEpjxjg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Postnatal_development_of_the_eye_in_the_naked_mole_rat_Heterocephalus_glaber_","translated_slug":"","page_count":21,"language":"en","content_type":"Work","summary":"The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be visually nonfunctional and as such is an ideal animal with which to pursue questions in evolutionary developmental biology. This report is the first in-depth study on the development and morphology of the naked mole rat eye. Using standard histological analysis and scanning and transmission electron microscopy, we describe the structural features of the eye. We further report on the morphological changes that accompany the development of this eye from neonate to adult and compare them with those that occur during mouse eye development. We observed numerous abnormalities in the shape and cellular arrangement of the structures of the anterior chamber, with notable malformations of the lens. Cell proliferation and cell death assays were conducted to investigate the possible causes of lens malformation. 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The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a DCT transcript of 3.5 kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken DCT gene consists of several introns and spans between 15 and 30 kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="056dd8cf6d27385405d9fc35d997277a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":107544899,"asset_id":109411763,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/107544899/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="109411763"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="109411763"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 109411763; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=109411763]").text(description); $(".js-view-count[data-work-id=109411763]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 109411763; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='109411763']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "056dd8cf6d27385405d9fc35d997277a" } } $('.js-work-strip[data-work-id=109411763]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":109411763,"title":"Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"We have cloned and sequenced a chicken cDNA encoding an -DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a DCT transcript of 3.5 kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken DCT gene consists of several introns and spans between 15 and 30 kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.","publication_date":{"day":null,"month":null,"year":1998,"errors":{}},"publication_name":"Gene","grobid_abstract_attachment_id":107544899},"translated_abstract":null,"internal_url":"https://www.academia.edu/109411763/Molecular_cloning_and_sequence_analysis_of_a_chicken_cDNA_encoding_tyrosinase_related_protein_2_DOPAchrome_tautomerase","translated_internal_url":"","created_at":"2023-11-19T14:06:08.484-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":107544899,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/107544899/thumbnails/1.jpg","file_name":"350b3b78c420f6cd8bfa2ab10d011a9b9422.pdf","download_url":"https://www.academia.edu/attachments/107544899/download_file","bulk_download_file_name":"Molecular_cloning_and_sequence_analysis.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/107544899/350b3b78c420f6cd8bfa2ab10d011a9b9422-libre.pdf?1700434318=\u0026response-content-disposition=attachment%3B+filename%3DMolecular_cloning_and_sequence_analysis.pdf\u0026Expires=1742176506\u0026Signature=FdJP8HGehgzSMbqSW84oIDk57KotoDwq-QzxCUC-a-knPAINDGV0AzqyWQmFOvePW04uIzNOwerfcW1zkgKuLxv8kb8oEsZlseOKAOwJOYeqEoXCgxV9mt7wGYNrFgMfIIMYauXV-y74V-mGI0ioB6AtEFfiEnqNK46HMtfZPgd6m3DW5Y2v1ECrv70i0A5YrNsoAbFyhTe3Hq5IF256xLxRwlh4UvNlPpxKGGwWEHnNa5XjAMsmorrk42UhnPV5mWpMRuSvBoswB6d60LG~pJLUx1YQgmLhxp8MdI5C7atGlGWmtMotuX2dgFS26K~zpkShn0-h9NiVIpXaCZJtCQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Molecular_cloning_and_sequence_analysis_of_a_chicken_cDNA_encoding_tyrosinase_related_protein_2_DOPAchrome_tautomerase","translated_slug":"","page_count":9,"language":"en","content_type":"Work","summary":"We have cloned and sequenced a chicken cDNA encoding an -DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a DCT transcript of 3.5 kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken DCT gene consists of several introns and spans between 15 and 30 kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":107544899,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/107544899/thumbnails/1.jpg","file_name":"350b3b78c420f6cd8bfa2ab10d011a9b9422.pdf","download_url":"https://www.academia.edu/attachments/107544899/download_file","bulk_download_file_name":"Molecular_cloning_and_sequence_analysis.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/107544899/350b3b78c420f6cd8bfa2ab10d011a9b9422-libre.pdf?1700434318=\u0026response-content-disposition=attachment%3B+filename%3DMolecular_cloning_and_sequence_analysis.pdf\u0026Expires=1742176506\u0026Signature=FdJP8HGehgzSMbqSW84oIDk57KotoDwq-QzxCUC-a-knPAINDGV0AzqyWQmFOvePW04uIzNOwerfcW1zkgKuLxv8kb8oEsZlseOKAOwJOYeqEoXCgxV9mt7wGYNrFgMfIIMYauXV-y74V-mGI0ioB6AtEFfiEnqNK46HMtfZPgd6m3DW5Y2v1ECrv70i0A5YrNsoAbFyhTe3Hq5IF256xLxRwlh4UvNlPpxKGGwWEHnNa5XjAMsmorrk42UhnPV5mWpMRuSvBoswB6d60LG~pJLUx1YQgmLhxp8MdI5C7atGlGWmtMotuX2dgFS26K~zpkShn0-h9NiVIpXaCZJtCQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning"},{"id":329263,"name":"Epidermal Growth Factor","url":"https://www.academia.edu/Documents/in/Epidermal_Growth_Factor"},{"id":371424,"name":"Genomic DNA","url":"https://www.academia.edu/Documents/in/Genomic_DNA"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":569436,"name":"Genomic Library","url":"https://www.academia.edu/Documents/in/Genomic_Library"},{"id":602609,"name":"Intron","url":"https://www.academia.edu/Documents/in/Intron"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":999803,"name":"Gene Family","url":"https://www.academia.edu/Documents/in/Gene_Family"},{"id":1578793,"name":"cDNA library","url":"https://www.academia.edu/Documents/in/cDNA_library"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"},{"id":3675689,"name":"Chick embryo","url":"https://www.academia.edu/Documents/in/Chick_embryo"}],"urls":[{"id":35559960,"url":"https://api.elsevier.com/content/article/PII:S037811199800403X?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="109411761"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/109411761/The_cloning_and_sequencing_of_a_cDNA_coding_for_chick_tyrosinase_related_protein_1"><img alt="Research paper thumbnail of The cloning and sequencing of a cDNA coding for chick tyrosinase-related protein-1" class="work-thumbnail" src="https://attachments.academia-assets.com/107544889/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/109411761/The_cloning_and_sequencing_of_a_cDNA_coding_for_chick_tyrosinase_related_protein_1">The cloning and sequencing of a cDNA coding for chick tyrosinase-related protein-1</a></div><div class="wp-workCard_item"><span>Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression</span><span>, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have cloned a cDNA encoding an avian homologue of the mammalian brownr TYRP1 locus protein. Th...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have cloned a cDNA encoding an avian homologue of the mammalian brownr TYRP1 locus protein. The chick Ž. tyrosinase-related protein-1 TRP-1 gene encodes a deduced protein of 535 amino acids, shares) 65% amino acid sequence identity with fish and mammalian TRP-1 proteins, and spans 5-11 kb of the chick genome. q 1998 Elsevier Science B.V.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="053bf98b3f4b1533506c81f367add8cf" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":107544889,"asset_id":109411761,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/107544889/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="109411761"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="109411761"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 109411761; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=109411761]").text(description); $(".js-view-count[data-work-id=109411761]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 109411761; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='109411761']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "053bf98b3f4b1533506c81f367add8cf" } } $('.js-work-strip[data-work-id=109411761]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":109411761,"title":"The cloning and sequencing of a cDNA coding for chick tyrosinase-related protein-1","translated_title":"","metadata":{"publisher":"Elsevier BV","ai_title_tag":"Cloning and Sequencing of Chick TRP-1 cDNA","grobid_abstract":"We have cloned a cDNA encoding an avian homologue of the mammalian brownr TYRP1 locus protein. The chick Ž. tyrosinase-related protein-1 TRP-1 gene encodes a deduced protein of 535 amino acids, shares) 65% amino acid sequence identity with fish and mammalian TRP-1 proteins, and spans 5-11 kb of the chick genome. q 1998 Elsevier Science B.V.","publication_date":{"day":null,"month":null,"year":1998,"errors":{}},"publication_name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","grobid_abstract_attachment_id":107544889},"translated_abstract":null,"internal_url":"https://www.academia.edu/109411761/The_cloning_and_sequencing_of_a_cDNA_coding_for_chick_tyrosinase_related_protein_1","translated_internal_url":"","created_at":"2023-11-19T14:06:07.292-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":107544889,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/107544889/thumbnails/1.jpg","file_name":"s0167-478128972900144-920231119-1-lj9rcq.pdf","download_url":"https://www.academia.edu/attachments/107544889/download_file","bulk_download_file_name":"The_cloning_and_sequencing_of_a_cDNA_cod.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/107544889/s0167-478128972900144-920231119-1-lj9rcq-libre.pdf?1700434315=\u0026response-content-disposition=attachment%3B+filename%3DThe_cloning_and_sequencing_of_a_cDNA_cod.pdf\u0026Expires=1742176506\u0026Signature=KqM8o1OnfiEbMx8EKY2gZu2pZTFj~rOz9PPSv11pD2bOKnIf25DGsDv965wDMNqolTAOKlPtPpurkD7eTC30FNu7XUadiMpPRSbMaq4Ag0BsZDRAvSk833Oh76rNuVw8WO2E~4SE8CVp-AcdN~o5zroQbW0GPYj4Y7dVHlrZwND6PNRuPCmVqCprRBdOV7KPBGaXOTWqYGA4Leycp4mv8veKaJ2ZmaBhMXeK~cSQQ1xIBfzm7zcMRhbvVAt4yMtugy6tbXgIx~tZSMYdl0271SIeT~G8OKQd7lYQZrl4SS8EeTdUB8dLN5-Gwjo~PZHRLSNTpNgt5Eez0E9HuA7LZg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_cloning_and_sequencing_of_a_cDNA_coding_for_chick_tyrosinase_related_protein_1","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"We have cloned a cDNA encoding an avian homologue of the mammalian brownr TYRP1 locus protein. The chick Ž. tyrosinase-related protein-1 TRP-1 gene encodes a deduced protein of 535 amino acids, shares) 65% amino acid sequence identity with fish and mammalian TRP-1 proteins, and spans 5-11 kb of the chick genome. q 1998 Elsevier Science B.V.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":107544889,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/107544889/thumbnails/1.jpg","file_name":"s0167-478128972900144-920231119-1-lj9rcq.pdf","download_url":"https://www.academia.edu/attachments/107544889/download_file","bulk_download_file_name":"The_cloning_and_sequencing_of_a_cDNA_cod.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/107544889/s0167-478128972900144-920231119-1-lj9rcq-libre.pdf?1700434315=\u0026response-content-disposition=attachment%3B+filename%3DThe_cloning_and_sequencing_of_a_cDNA_cod.pdf\u0026Expires=1742176506\u0026Signature=KqM8o1OnfiEbMx8EKY2gZu2pZTFj~rOz9PPSv11pD2bOKnIf25DGsDv965wDMNqolTAOKlPtPpurkD7eTC30FNu7XUadiMpPRSbMaq4Ag0BsZDRAvSk833Oh76rNuVw8WO2E~4SE8CVp-AcdN~o5zroQbW0GPYj4Y7dVHlrZwND6PNRuPCmVqCprRBdOV7KPBGaXOTWqYGA4Leycp4mv8veKaJ2ZmaBhMXeK~cSQQ1xIBfzm7zcMRhbvVAt4yMtugy6tbXgIx~tZSMYdl0271SIeT~G8OKQd7lYQZrl4SS8EeTdUB8dLN5-Gwjo~PZHRLSNTpNgt5Eez0E9HuA7LZg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA"},{"id":112338,"name":"Oxidoreductases","url":"https://www.academia.edu/Documents/in/Oxidoreductases"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene"},{"id":233229,"name":"Genes","url":"https://www.academia.edu/Documents/in/Genes"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":585573,"name":"Amino Acid Profile","url":"https://www.academia.edu/Documents/in/Amino_Acid_Profile"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":899568,"name":"Tyrosinase","url":"https://www.academia.edu/Documents/in/Tyrosinase"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"}],"urls":[{"id":35559959,"url":"https://api.elsevier.com/content/article/PII:S0167478197001449?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="109411758"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/109411758/Transcriptional_and_electrophysiological_aberrations_in_an_induced_pluripotent_stem_cell_derived_model_of_spinocerebellar_ataxia_type_7"><img alt="Research paper thumbnail of Transcriptional and electrophysiological aberrations in an induced pluripotent stem cell-derived model of spinocerebellar ataxia type 7" class="work-thumbnail" src="https://attachments.academia-assets.com/107544887/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/109411758/Transcriptional_and_electrophysiological_aberrations_in_an_induced_pluripotent_stem_cell_derived_model_of_spinocerebellar_ataxia_type_7">Transcriptional and electrophysiological aberrations in an induced pluripotent stem cell-derived model of spinocerebellar ataxia type 7</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Spinocerebellar ataxia type 7 (SCA7) is an inherited neurodegenerative disease that is characteri...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Spinocerebellar ataxia type 7 (SCA7) is an inherited neurodegenerative disease that is characterised by ataxia and visual loss. It results from a degeneration of cerebellar Purkinje neurons and retinal photoreceptors caused by a polyglutamine repeat expansion in the ATXN7 gene, a component of the STAGA transcription co-activator complex. As with many neurodegenerative diseases, studies of pathogenesis have been hindered by a lack of disease-relevant models. To this end, we have generated the first induced pluripotent stem cells (iPSCs) from South African SCA7 patients, where the disease occurs at an unusually high frequency as a result of a founder effect. These iPSCs were capable of differentiation into neural and retinal cells, and showed evidence of a transcriptional phenotype affecting components of STAGA (ATXN7 and KAT2A) and the heat shock protein pathway (DNAJA1 and HSP70). Functionally, SCA7 iPSC-derived neurons exhibited more negative resting membrane potentials and increas...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6bed4b0c761d150a2d94789afc808a58" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":107544887,"asset_id":109411758,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/107544887/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="109411758"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="109411758"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 109411758; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=109411758]").text(description); $(".js-view-count[data-work-id=109411758]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 109411758; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='109411758']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6bed4b0c761d150a2d94789afc808a58" } } $('.js-work-strip[data-work-id=109411758]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":109411758,"title":"Transcriptional and electrophysiological aberrations in an induced pluripotent stem cell-derived model of spinocerebellar ataxia type 7","translated_title":"","metadata":{"abstract":"Spinocerebellar ataxia type 7 (SCA7) is an inherited neurodegenerative disease that is characterised by ataxia and visual loss. 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Although photodynamic mechanisms (i.e. through endogenous photosensitizers) play a role in UVA phototherapy for the treatment of skin disorders such as eczema and psoriasis, photodynamic therapy employing exogenous photosensitizers are currently being used only for the treatment of certain forms of non-melanoma skin cancers and actinic keratoses. There are few reports however on its use in treating melanomas. This in vitro study analyses the phototoxic effect of UVA (400-315 nm)-activated hypericin in human pigmented and unpigmented melanomas and immortalised keratinocytes and melanocytes. We show that neither hypericin exposure nor UV irradiation alone reduces cell viability. We show that an exposure to 1 lM UVA-activated hypericin does not bring about cell death, while 3 lM activated hypericin induces a necrotic mode of cell death in pigmented melanoma cells and melanocytes and an apoptotic mode of cell death in non-pigmented melanoma cells and keratinocytes. We hypothesis that the necrotic mode of cell death in the pigmented cells is possibly related to the presence of melanin-containing melanosomes in these cells and that the hypericin-induced increase in reactive oxygen species leads to an increase in permeability of melanosomes. This would result in toxic melanin precursors (of an indolic and phenolic nature) leaking into the cytoplasm which in turn leads to cell death. Hypericin localisation in the endoplasmic reticulum in these cells shown by fluorescent microscopy, further support a disruption in cellular processing and induction of cell death. In contrast, this study shows that cells that do not contain melanosomes (non-pigmented melanoma cells and keratinocytes) die by apoptosis. Further, using a mitochondrial-specific fluorescent dye, we show that intracellular accumulation of hypericin induces a mitochondrial-associated caspase-dependent apoptotic mode of cell death. This work suggests that UVA is effective in activating hypericin and that this phototoxicity may be considered as treatment option in some cases of lentigo maligna or lentigo maligna melanoma that are too large for surgical resection.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ee5c0b1a96cf13e4337dc9d305922df5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":96615731,"asset_id":94047031,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/96615731/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94047031"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94047031"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94047031; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94047031]").text(description); $(".js-view-count[data-work-id=94047031]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94047031; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94047031']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ee5c0b1a96cf13e4337dc9d305922df5" } } $('.js-work-strip[data-work-id=94047031]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94047031,"title":"Hypericin phototoxicity induces different modes of cell death in melanoma and human skin cells","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Hypericin, the major component of St. John's Wort, absorbs light in the UV and visible ranges whereupon it becomes phototoxic through the production of reactive oxygen species. Although photodynamic mechanisms (i.e. through endogenous photosensitizers) play a role in UVA phototherapy for the treatment of skin disorders such as eczema and psoriasis, photodynamic therapy employing exogenous photosensitizers are currently being used only for the treatment of certain forms of non-melanoma skin cancers and actinic keratoses. There are few reports however on its use in treating melanomas. This in vitro study analyses the phototoxic effect of UVA (400-315 nm)-activated hypericin in human pigmented and unpigmented melanomas and immortalised keratinocytes and melanocytes. We show that neither hypericin exposure nor UV irradiation alone reduces cell viability. We show that an exposure to 1 lM UVA-activated hypericin does not bring about cell death, while 3 lM activated hypericin induces a necrotic mode of cell death in pigmented melanoma cells and melanocytes and an apoptotic mode of cell death in non-pigmented melanoma cells and keratinocytes. We hypothesis that the necrotic mode of cell death in the pigmented cells is possibly related to the presence of melanin-containing melanosomes in these cells and that the hypericin-induced increase in reactive oxygen species leads to an increase in permeability of melanosomes. This would result in toxic melanin precursors (of an indolic and phenolic nature) leaking into the cytoplasm which in turn leads to cell death. Hypericin localisation in the endoplasmic reticulum in these cells shown by fluorescent microscopy, further support a disruption in cellular processing and induction of cell death. In contrast, this study shows that cells that do not contain melanosomes (non-pigmented melanoma cells and keratinocytes) die by apoptosis. Further, using a mitochondrial-specific fluorescent dye, we show that intracellular accumulation of hypericin induces a mitochondrial-associated caspase-dependent apoptotic mode of cell death. 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Although photodynamic mechanisms (i.e. through endogenous photosensitizers) play a role in UVA phototherapy for the treatment of skin disorders such as eczema and psoriasis, photodynamic therapy employing exogenous photosensitizers are currently being used only for the treatment of certain forms of non-melanoma skin cancers and actinic keratoses. There are few reports however on its use in treating melanomas. This in vitro study analyses the phototoxic effect of UVA (400-315 nm)-activated hypericin in human pigmented and unpigmented melanomas and immortalised keratinocytes and melanocytes. We show that neither hypericin exposure nor UV irradiation alone reduces cell viability. We show that an exposure to 1 lM UVA-activated hypericin does not bring about cell death, while 3 lM activated hypericin induces a necrotic mode of cell death in pigmented melanoma cells and melanocytes and an apoptotic mode of cell death in non-pigmented melanoma cells and keratinocytes. We hypothesis that the necrotic mode of cell death in the pigmented cells is possibly related to the presence of melanin-containing melanosomes in these cells and that the hypericin-induced increase in reactive oxygen species leads to an increase in permeability of melanosomes. This would result in toxic melanin precursors (of an indolic and phenolic nature) leaking into the cytoplasm which in turn leads to cell death. Hypericin localisation in the endoplasmic reticulum in these cells shown by fluorescent microscopy, further support a disruption in cellular processing and induction of cell death. In contrast, this study shows that cells that do not contain melanosomes (non-pigmented melanoma cells and keratinocytes) die by apoptosis. Further, using a mitochondrial-specific fluorescent dye, we show that intracellular accumulation of hypericin induces a mitochondrial-associated caspase-dependent apoptotic mode of cell death. This work suggests that UVA is effective in activating hypericin and that this phototoxicity may be considered as treatment option in some cases of lentigo maligna or lentigo maligna melanoma that are too large for surgical resection.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":96615731,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96615731/thumbnails/1.jpg","file_name":"j.jphotobiol.2008.01.01120221231-1-1aiv87j.pdf","download_url":"https://www.academia.edu/attachments/96615731/download_file","bulk_download_file_name":"Hypericin_phototoxicity_induces_differen.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96615731/j.jphotobiol.2008.01.01120221231-1-1aiv87j-libre.pdf?1672492207=\u0026response-content-disposition=attachment%3B+filename%3DHypericin_phototoxicity_induces_differen.pdf\u0026Expires=1742176506\u0026Signature=XSuI8b56cnr64Mua3-kudRQzO1sbyjx75pR5JQe7c0231QdsKR-KcjYImuL~9sz2oL~ab2wx1k9o1figXMv~bkgNIbQxST~8iMEdJBrBNpoTNjJHLXJibkeXPr6RwyK2CFdo0Qm50Kk6iSha9v6JSwdfZi~uHnFvzoGpwgDGYHibsrp98YVKq4bROjOZM90pXW6~j8RGzvvRzkyCD-GNRH2ENM~Az3ECT4Wwp1ZlEb0k0OFG2YGUYDtbGVtnrP5ioQUOvLsYcDSuWqFx9lJIYuQFG2ck0u5SrmtWVFEN199T6mmQSN0S~27MAQgxGYvDFiEIjxo2J1kAcj3CcbkXxA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7631,"name":"Melanin","url":"https://www.academia.edu/Documents/in/Melanin"},{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":70552,"name":"Keratinocytes","url":"https://www.academia.edu/Documents/in/Keratinocytes"},{"id":99023,"name":"Melanoma","url":"https://www.academia.edu/Documents/in/Melanoma"},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death"},{"id":122187,"name":"Endoplasmic Reticulum","url":"https://www.academia.edu/Documents/in/Endoplasmic_Reticulum"},{"id":165002,"name":"Cell Viability","url":"https://www.academia.edu/Documents/in/Cell_Viability"},{"id":247477,"name":"Caspase","url":"https://www.academia.edu/Documents/in/Caspase"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":780594,"name":"In Vitro Studies","url":"https://www.academia.edu/Documents/in/In_Vitro_Studies"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":1311259,"name":"Intracellular Space","url":"https://www.academia.edu/Documents/in/Intracellular_Space"},{"id":1344396,"name":"Hypericin","url":"https://www.academia.edu/Documents/in/Hypericin"},{"id":1348005,"name":"Melanosome","url":"https://www.academia.edu/Documents/in/Melanosome"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"},{"id":3826324,"name":"Human skin","url":"https://www.academia.edu/Documents/in/Human_skin-1"}],"urls":[{"id":27605768,"url":"https://api.elsevier.com/content/article/PII:S101113440800033X?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94047028"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94047028/Immunofluorescent_Identification_of_Melanocytes_in_Murine_Hair_Follicles"><img alt="Research paper thumbnail of Immunofluorescent Identification of Melanocytes in Murine Hair Follicles" class="work-thumbnail" src="https://attachments.academia-assets.com/96615709/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94047028/Immunofluorescent_Identification_of_Melanocytes_in_Murine_Hair_Follicles">Immunofluorescent Identification of Melanocytes in Murine Hair Follicles</a></div><div class="wp-workCard_item"><span>Journal of Molecular Histology</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluorescent components within the cell and the environment. This is particularly evident in hair follicles. This paper reports on a serendipitous modification to an existing method which results in a drastically reduced background fluorescence. Immediately after antigen retrieval, sections exposed to 0.3% hydrogen peroxide in methanol for 30 min at room temperature exhibited low background fluorescence, increased antigenicity and revealed quantifiable numbers of melanocytes. This method is applicable to both human and mouse melanocytes particularly in the hair follicle.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="df6379294e603c5581003b8e09f7807a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":96615709,"asset_id":94047028,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/96615709/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94047028"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94047028"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94047028; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94047028]").text(description); $(".js-view-count[data-work-id=94047028]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94047028; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94047028']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "df6379294e603c5581003b8e09f7807a" } } $('.js-work-strip[data-work-id=94047028]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94047028,"title":"Immunofluorescent Identification of Melanocytes in Murine Hair Follicles","translated_title":"","metadata":{"publisher":"Springer Science and Business Media LLC","ai_title_tag":"Reduced Background Immunofluorescence in Hair Follicles","grobid_abstract":"Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluorescent components within the cell and the environment. 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This method is applicable to both human and mouse melanocytes particularly in the hair follicle.","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Journal of Molecular Histology","grobid_abstract_attachment_id":96615709},"translated_abstract":null,"internal_url":"https://www.academia.edu/94047028/Immunofluorescent_Identification_of_Melanocytes_in_Murine_Hair_Follicles","translated_internal_url":"","created_at":"2022-12-31T05:07:06.254-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":96615709,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96615709/thumbnails/1.jpg","file_name":"s10735-005-9011-820221231-1-1c3byhy.pdf","download_url":"https://www.academia.edu/attachments/96615709/download_file","bulk_download_file_name":"Immunofluorescent_Identification_of_Mela.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96615709/s10735-005-9011-820221231-1-1c3byhy-libre.pdf?1672492204=\u0026response-content-disposition=attachment%3B+filename%3DImmunofluorescent_Identification_of_Mela.pdf\u0026Expires=1742176506\u0026Signature=SwER-YyyUhRmOOPVdxg2IAGdmsYvZLEfOEYmpm4di~mBXBOS7Jrd8~8S0hkXiEivruCUqTQ2~dd7W1egz6fBWvybMBIIfV~kaegX-Od~c7GeNNCNXrTCY-kJ8LqiRKyKRHX-3MJ0qQ7zl34reManhAtUfACMYsfOugHCWe-oT8XuIYpW0nYpZ0ovOKmfZUtauSRKYJidD1i8K8BGOUfvBN222x0moXhf4r-R~GJ6vKCYAbBInGS-gqKo1SX3nSLb-rsy25p9bA7SfEMqyhMzcTcv2FQB4Gc1ktTS-WuzjHSRRQ9FtzUCcGeZQYMU-PW1y6aGpQ8TwGdKrILdhEhXXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Immunofluorescent_Identification_of_Melanocytes_in_Murine_Hair_Follicles","translated_slug":"","page_count":3,"language":"en","content_type":"Work","summary":"Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluorescent components within the cell and the environment. This is particularly evident in hair follicles. This paper reports on a serendipitous modification to an existing method which results in a drastically reduced background fluorescence. Immediately after antigen retrieval, sections exposed to 0.3% hydrogen peroxide in methanol for 30 min at room temperature exhibited low background fluorescence, increased antigenicity and revealed quantifiable numbers of melanocytes. This method is applicable to both human and mouse melanocytes particularly in the hair follicle.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":96615709,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96615709/thumbnails/1.jpg","file_name":"s10735-005-9011-820221231-1-1c3byhy.pdf","download_url":"https://www.academia.edu/attachments/96615709/download_file","bulk_download_file_name":"Immunofluorescent_Identification_of_Mela.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96615709/s10735-005-9011-820221231-1-1c3byhy-libre.pdf?1672492204=\u0026response-content-disposition=attachment%3B+filename%3DImmunofluorescent_Identification_of_Mela.pdf\u0026Expires=1742176506\u0026Signature=SwER-YyyUhRmOOPVdxg2IAGdmsYvZLEfOEYmpm4di~mBXBOS7Jrd8~8S0hkXiEivruCUqTQ2~dd7W1egz6fBWvybMBIIfV~kaegX-Od~c7GeNNCNXrTCY-kJ8LqiRKyKRHX-3MJ0qQ7zl34reManhAtUfACMYsfOugHCWe-oT8XuIYpW0nYpZ0ovOKmfZUtauSRKYJidD1i8K8BGOUfvBN222x0moXhf4r-R~GJ6vKCYAbBInGS-gqKo1SX3nSLb-rsy25p9bA7SfEMqyhMzcTcv2FQB4Gc1ktTS-WuzjHSRRQ9FtzUCcGeZQYMU-PW1y6aGpQ8TwGdKrILdhEhXXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide"},{"id":391252,"name":"Room Temperature","url":"https://www.academia.edu/Documents/in/Room_Temperature"},{"id":568482,"name":"Biological markers","url":"https://www.academia.edu/Documents/in/Biological_markers"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":1123231,"name":"Hair Follicle","url":"https://www.academia.edu/Documents/in/Hair_Follicle"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"},{"id":2007902,"name":"Antigenicity","url":"https://www.academia.edu/Documents/in/Antigenicity"},{"id":3789879,"name":"Cardiovascular medicine and haematology","url":"https://www.academia.edu/Documents/in/Cardiovascular_medicine_and_haematology"}],"urls":[{"id":27605765,"url":"http://link.springer.com/content/pdf/10.1007/s10735-005-9011-8.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94046999"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94046999/The_effect_of_temperature_on_tyrosinase_activity_in_Himalayan_mouse_skin"><img alt="Research paper thumbnail of The effect of temperature on tyrosinase activity in Himalayan mouse skin" class="work-thumbnail" src="https://attachments.academia-assets.com/96615673/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94046999/The_effect_of_temperature_on_tyrosinase_activity_in_Himalayan_mouse_skin">The effect of temperature on tyrosinase activity in Himalayan mouse skin</a></div><div class="wp-workCard_item"><span>The Journal of experimental zoology</span><span>, 1981</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The tyrosinase activity of Himalayan mouse skin homogenates was measured over a range of temperat...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The tyrosinase activity of Himalayan mouse skin homogenates was measured over a range of temperatures using two sensitive radiometric assay--namely, (1) the measurement of 14C-tyrosine incorporation into melanin, and (2) the measurement of 3HOH released as a by-product of 3H-tyrosine hydroxylation. Results show that Himalayan tyrosinase is maximally active at temperatures well below normal body temperature (15 degree C to 25 degree C). These results are in support of Danneel&#39;s visual observations (&#39;41) that &quot;ferment&quot; activity of Himalayan rabbit skin is absent at temperatures above 25 degree C. Further results suggest the presence of a tyrosinase inhibitor in Himalayan mouse skin. First, removal of a low molecular weight fraction from Himalayan skin homogenates resulted in an increase in tyrosinase activity. Second, recombination of the low molecular weight fraction to the homogenate from which it was originally separated resulted in a decrease in tyrosinase activi...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0e81a4a3865353bdf8ad06eaf9a45959" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":96615673,"asset_id":94046999,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/96615673/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94046999"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94046999"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94046999; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94046999]").text(description); $(".js-view-count[data-work-id=94046999]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94046999; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94046999']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0e81a4a3865353bdf8ad06eaf9a45959" } } $('.js-work-strip[data-work-id=94046999]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94046999,"title":"The effect of temperature on tyrosinase activity in Himalayan mouse skin","translated_title":"","metadata":{"abstract":"The tyrosinase activity of Himalayan mouse skin homogenates was measured over a range of temperatures using two sensitive radiometric assay--namely, (1) the measurement of 14C-tyrosine incorporation into melanin, and (2) the measurement of 3HOH released as a by-product of 3H-tyrosine hydroxylation. Results show that Himalayan tyrosinase is maximally active at temperatures well below normal body temperature (15 degree C to 25 degree C). These results are in support of Danneel\u0026#39;s visual observations (\u0026#39;41) that \u0026quot;ferment\u0026quot; activity of Himalayan rabbit skin is absent at temperatures above 25 degree C. Further results suggest the presence of a tyrosinase inhibitor in Himalayan mouse skin. First, removal of a low molecular weight fraction from Himalayan skin homogenates resulted in an increase in tyrosinase activity. 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These results are in support of Danneel\u0026#39;s visual observations (\u0026#39;41) that \u0026quot;ferment\u0026quot; activity of Himalayan rabbit skin is absent at temperatures above 25 degree C. Further results suggest the presence of a tyrosinase inhibitor in Himalayan mouse skin. First, removal of a low molecular weight fraction from Himalayan skin homogenates resulted in an increase in tyrosinase activity. 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Although accepted as dogma, t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Vitiligo is a depigmenting disease of uncertain aetio-pathogenesis. Although accepted as dogma, the question of whether melanocytes survive in vitiligo lesions has not been adequately resolved. Defining with greater accuracy the melanocyte status of lesions would contribute greatly towards the understanding of the etiology, progression and treatment of this disorder. We have therefore revisited this issue by carrying out a molecular screen for melanocytes in lesional skin using the sensitive and specific technique of reverse transcription PCR (RT-PCR) followed by Southern blotting. Biopsies from vitiligo lesions and normal skin were obtained from 15 patients. The RT-PCR was carried out using primers for tyrosinase and dopa-chrome tautomerase (DCT). To increase the sensitivity of detection, Southern-blot analysis of all PCR products was conducted. Southern-blot analysis indicated that three lesional samples were positive: one for tyrosinase, one for DCT, and one for both. Lesions yielding positive results had been present for between 2-5 years and were inactive, as defined by no disease progression within the last 3 months. Some vitiligo lesions showed evidence of melanocyte survival, even after some years. These results open the way for the possibility of using a range of melanocyte-specific markers for molecular staging of lesional status by quantitative RT-PCR. Such information would be extremely valuable for the appropriate selection and potential success of medical therapies.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546066"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546066"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546066; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546066]").text(description); $(".js-view-count[data-work-id=83546066]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546066; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546066']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=83546066]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546066,"title":"Molecular analysis of vitiligo lesions reveals sporadic melanocyte survival","translated_title":"","metadata":{"abstract":"Vitiligo is a depigmenting disease of uncertain aetio-pathogenesis. 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Some vitiligo lesions showed evidence of melanocyte survival, even after some years. These results open the way for the possibility of using a range of melanocyte-specific markers for molecular staging of lesional status by quantitative RT-PCR. Such information would be extremely valuable for the appropriate selection and potential success of medical therapies.","internal_url":"https://www.academia.edu/83546066/Molecular_analysis_of_vitiligo_lesions_reveals_sporadic_melanocyte_survival","translated_internal_url":"","created_at":"2022-07-22T02:43:47.220-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Molecular_analysis_of_vitiligo_lesions_reveals_sporadic_melanocyte_survival","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Vitiligo is a depigmenting disease of uncertain aetio-pathogenesis. Although accepted as dogma, the question of whether melanocytes survive in vitiligo lesions has not been adequately resolved. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546064"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/83546064/The_three_dimensional_organisation_of_the_post_trabecular_aqueous_outflow_pathway_and_limbal_vasculature_in_the_mouse"><img alt="Research paper thumbnail of The three-dimensional organisation of the post-trabecular aqueous outflow pathway and limbal vasculature in the mouse" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/83546064/The_three_dimensional_organisation_of_the_post_trabecular_aqueous_outflow_pathway_and_limbal_vasculature_in_the_mouse">The three-dimensional organisation of the post-trabecular aqueous outflow pathway and limbal vasculature in the mouse</a></div><div class="wp-workCard_item"><span>Experimental eye research</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The mouse eye has been used as a model for studies on the microanatomy of the outflow pathways bu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The mouse eye has been used as a model for studies on the microanatomy of the outflow pathways but most of what is known comes from histological sections. These studies have focused mainly on the morphological features of the trabecular meshwork, Schlemm&#39;s canal and aqueous channels that link to the superficial episcleral vasculature. However, the anatomical architecture of the aqueous outflow vessels and their relationship to each other and to the general vascular circulation is not well understood. The aim of this study was to provide a detailed description of the microarchitecture of the aqueous outflow vessels and their relationship to the superficial limbal/episcleral vasculature throughout the entire limbus. The aqueous outflow vessels and blood and lymphatic vessels were imaged in PECAM-1 and LYVE-1 immunostained whole anterior segments of adult mice and three-dimensional (3-D) reconstructions of the optical sections were generated to reveal the aqueous, blood and lymphat...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546064"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546064"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546064; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546064]").text(description); $(".js-view-count[data-work-id=83546064]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546064; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546064']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=83546064]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546064,"title":"The three-dimensional organisation of the post-trabecular aqueous outflow pathway and limbal vasculature in the mouse","translated_title":"","metadata":{"abstract":"The mouse eye has been used as a model for studies on the microanatomy of the outflow pathways but most of what is known comes from histological sections. 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The aim of this study was to provide a detailed description of the microarchitecture of the aqueous outflow vessels and their relationship to the superficial limbal/episcleral vasculature throughout the entire limbus. 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Since pigment synthesis only occurs in actively growing hair, adult mice were plucked to induce hair growth. The extent of darkening of the hair was recorded by photography against a reference scale. The presence of pigment granules in hair follicles was investigated histologically. Housing adult and juvenile mice at 15 degrees C results in the synthesis of pigment in growing hair follicles whereas housing at 30 degrees C results in the absence of pigment granules in the growing hair follicles.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b942c9a047e8ec04851c8a7c00d0c7ba" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854364,"asset_id":83546062,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854364/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546062"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546062"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546062; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546062]").text(description); $(".js-view-count[data-work-id=83546062]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546062; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546062']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b942c9a047e8ec04851c8a7c00d0c7ba" } } $('.js-work-strip[data-work-id=83546062]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546062,"title":"Pigment synthesis in the Himalayan mouse","translated_title":"","metadata":{"abstract":"The effect of temperature on pigment synthesis in adult and juvenile Himalayan mice was investigated. 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Housing adult and juvenile mice at 15 degrees C results in the synthesis of pigment in growing hair follicles whereas housing at 30 degrees C results in the absence of pigment granules in the growing hair follicles.","internal_url":"https://www.academia.edu/83546062/Pigment_synthesis_in_the_Himalayan_mouse","translated_internal_url":"","created_at":"2022-07-22T02:43:46.893-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854364,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854364/thumbnails/1.jpg","file_name":"jez.140210011620220722-1-l69ny2.pdf","download_url":"https://www.academia.edu/attachments/88854364/download_file","bulk_download_file_name":"Pigment_synthesis_in_the_Himalayan_mouse.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854364/jez.140210011620220722-1-l69ny2-libre.pdf?1658483330=\u0026response-content-disposition=attachment%3B+filename%3DPigment_synthesis_in_the_Himalayan_mouse.pdf\u0026Expires=1742176506\u0026Signature=HwNZ0xSPu9bRU7~GyeizAu~CzqefzTWZ1YcSz8Qr4tb1pzltPaINJq1AWRRL5LVdLKTrAqzHWPTb1h29gk9Y3JxQmbwegju8bDYO9qLgwS-1c0029LtQzaaSD3NlRhS-yvxXiFxMpXFl2tWxnFbYsDnHwSl1wZGoBP2gWOi~kGh-VJys1W5vSJne1MAyUxQFTWdBQn3C1AJCXfhaXtL3-c1mDiqmNHkLAxNvirYEtjLXIh8CKx9qxyTN1Yzrb0BT7MqvzCdQJxAllgAfU-hqgvWAAtbAWz~VUv~cyXgPErvsN4HcgzKvc3CNJDjSgrL6rNf7EOm8eM0NrQx5xVY12A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Pigment_synthesis_in_the_Himalayan_mouse","translated_slug":"","page_count":8,"language":"en","content_type":"Work","summary":"The effect of temperature on pigment synthesis in adult and juvenile Himalayan mice was investigated. Since pigment synthesis only occurs in actively growing hair, adult mice were plucked to induce hair growth. The extent of darkening of the hair was recorded by photography against a reference scale. The presence of pigment granules in hair follicles was investigated histologically. Housing adult and juvenile mice at 15 degrees C results in the synthesis of pigment in growing hair follicles whereas housing at 30 degrees C results in the absence of pigment granules in the growing hair follicles.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854364,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854364/thumbnails/1.jpg","file_name":"jez.140210011620220722-1-l69ny2.pdf","download_url":"https://www.academia.edu/attachments/88854364/download_file","bulk_download_file_name":"Pigment_synthesis_in_the_Himalayan_mouse.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854364/jez.140210011620220722-1-l69ny2-libre.pdf?1658483330=\u0026response-content-disposition=attachment%3B+filename%3DPigment_synthesis_in_the_Himalayan_mouse.pdf\u0026Expires=1742176506\u0026Signature=HwNZ0xSPu9bRU7~GyeizAu~CzqefzTWZ1YcSz8Qr4tb1pzltPaINJq1AWRRL5LVdLKTrAqzHWPTb1h29gk9Y3JxQmbwegju8bDYO9qLgwS-1c0029LtQzaaSD3NlRhS-yvxXiFxMpXFl2tWxnFbYsDnHwSl1wZGoBP2gWOi~kGh-VJys1W5vSJne1MAyUxQFTWdBQn3C1AJCXfhaXtL3-c1mDiqmNHkLAxNvirYEtjLXIh8CKx9qxyTN1Yzrb0BT7MqvzCdQJxAllgAfU-hqgvWAAtbAWz~VUv~cyXgPErvsN4HcgzKvc3CNJDjSgrL6rNf7EOm8eM0NrQx5xVY12A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry"},{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology"},{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":3770,"name":"Metabolism","url":"https://www.academia.edu/Documents/in/Metabolism"},{"id":7666,"name":"Life history","url":"https://www.academia.edu/Documents/in/Life_history"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":10866,"name":"Morphology","url":"https://www.academia.edu/Documents/in/Morphology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":83996,"name":"Hair","url":"https://www.academia.edu/Documents/in/Hair"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature"},{"id":162553,"name":"Skin","url":"https://www.academia.edu/Documents/in/Skin"},{"id":233229,"name":"Genes","url":"https://www.academia.edu/Documents/in/Genes"},{"id":260028,"name":"Unknown","url":"https://www.academia.edu/Documents/in/Unknown"},{"id":493957,"name":"Organs","url":"https://www.academia.edu/Documents/in/Organs"},{"id":576640,"name":"Hair Color","url":"https://www.academia.edu/Documents/in/Hair_Color"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":976256,"name":"Skin Temperature","url":"https://www.academia.edu/Documents/in/Skin_Temperature"},{"id":1312021,"name":"Environmental Exposure","url":"https://www.academia.edu/Documents/in/Environmental_Exposure"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546061"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546061/A_congenital_defect_of_the_distal_forelimb_of_a_cat_and_of_a_dog"><img alt="Research paper thumbnail of A congenital defect of the distal forelimb of a cat and of a dog" class="work-thumbnail" src="https://attachments.academia-assets.com/88854355/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546061/A_congenital_defect_of_the_distal_forelimb_of_a_cat_and_of_a_dog">A congenital defect of the distal forelimb of a cat and of a dog</a></div><div class="wp-workCard_item"><span>Journal of the South African Veterinary Association</span><span>, 2002</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f84f577ebe3e35e311dcebd06931e9e4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854355,"asset_id":83546061,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854355/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546061"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546061"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546061; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546061]").text(description); $(".js-view-count[data-work-id=83546061]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546061; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546061']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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The cases illustrate that the defects are likely the result of in utero damage leading to limb bifurcation rather than complete duplication, with molecular interactions influencing limb growth. The report provides radiographic evidence and discusses the implications on overall limb function and development.","publication_date":{"day":null,"month":null,"year":2002,"errors":{}},"publication_name":"Journal of the South African Veterinary Association"},"translated_abstract":null,"internal_url":"https://www.academia.edu/83546061/A_congenital_defect_of_the_distal_forelimb_of_a_cat_and_of_a_dog","translated_internal_url":"","created_at":"2022-07-22T02:43:46.760-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854355,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854355/thumbnails/1.jpg","file_name":"1d105c5ae20b617ddcd90bf06ca3a8d93bb3.pdf","download_url":"https://www.academia.edu/attachments/88854355/download_file","bulk_download_file_name":"A_congenital_defect_of_the_distal_foreli.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854355/1d105c5ae20b617ddcd90bf06ca3a8d93bb3-libre.pdf?1658483327=\u0026response-content-disposition=attachment%3B+filename%3DA_congenital_defect_of_the_distal_foreli.pdf\u0026Expires=1742176506\u0026Signature=IfHBCO0ara1401QvwrmFJXe3OHdkSjz7JKv0CfLFTnSaY4AX0NPhNB48V-WDvGkxR0uJbO3W0OXeJVZA4mklZBerP9xOHs-kIf0f4~iW2E8EOUrmKIbsjgDpL00wl5VX30WF4EPVjyumBQyUloOEuavWpX98kzXUJQtcIEA-dvz-z7MM-dhoZC0FOKc9PIzAi2hpq1~EfIa1h5xVN4B7jUK3I0tLRxzU34IDVBdboW1St266vsUCU~Jeas~coJcVoVUGaiiUOxowYESSXK1WTOa5J0iqJlwHQYWfSvlBKwC6xPailn5uYzqla1Tqr8OhSzVmqrM4Kn2bwjBWHdFwXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_congenital_defect_of_the_distal_forelimb_of_a_cat_and_of_a_dog","translated_slug":"","page_count":2,"language":"en","content_type":"Work","summary":null,"owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854355,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854355/thumbnails/1.jpg","file_name":"1d105c5ae20b617ddcd90bf06ca3a8d93bb3.pdf","download_url":"https://www.academia.edu/attachments/88854355/download_file","bulk_download_file_name":"A_congenital_defect_of_the_distal_foreli.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854355/1d105c5ae20b617ddcd90bf06ca3a8d93bb3-libre.pdf?1658483327=\u0026response-content-disposition=attachment%3B+filename%3DA_congenital_defect_of_the_distal_foreli.pdf\u0026Expires=1742176506\u0026Signature=IfHBCO0ara1401QvwrmFJXe3OHdkSjz7JKv0CfLFTnSaY4AX0NPhNB48V-WDvGkxR0uJbO3W0OXeJVZA4mklZBerP9xOHs-kIf0f4~iW2E8EOUrmKIbsjgDpL00wl5VX30WF4EPVjyumBQyUloOEuavWpX98kzXUJQtcIEA-dvz-z7MM-dhoZC0FOKc9PIzAi2hpq1~EfIa1h5xVN4B7jUK3I0tLRxzU34IDVBdboW1St266vsUCU~Jeas~coJcVoVUGaiiUOxowYESSXK1WTOa5J0iqJlwHQYWfSvlBKwC6xPailn5uYzqla1Tqr8OhSzVmqrM4Kn2bwjBWHdFwXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":41088,"name":"Cats","url":"https://www.academia.edu/Documents/in/Cats"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs"},{"id":54589,"name":"Anatomy","url":"https://www.academia.edu/Documents/in/Anatomy"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":4055611,"name":"Fatal outcome","url":"https://www.academia.edu/Documents/in/Fatal_outcome"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546060"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546060/A_New_Class_of_Stem_Cells_in_South_Africa_Introducing_Induced_Pluripotent_Stem_cells_iPS_cells_"><img alt="Research paper thumbnail of A New Class of Stem Cells in South Africa: Introducing Induced Pluripotent Stem cells (iPS cells)" class="work-thumbnail" src="https://attachments.academia-assets.com/88854356/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546060/A_New_Class_of_Stem_Cells_in_South_Africa_Introducing_Induced_Pluripotent_Stem_cells_iPS_cells_">A New Class of Stem Cells in South Africa: Introducing Induced Pluripotent Stem cells (iPS cells)</a></div><div class="wp-workCard_item"><span>South African Medical Journal</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="040bb7eeeefb485c5914e38d374b9f8b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854356,"asset_id":83546060,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854356/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546060"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546060"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546060; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546059"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546059/Closely_linked_early_and_late_histone_H2B_genes_are_differentially_expressed_after_microinjection_into_sea_urchin_zygotes"><img alt="Research paper thumbnail of Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes" class="work-thumbnail" src="https://attachments.academia-assets.com/88854358/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546059/Closely_linked_early_and_late_histone_H2B_genes_are_differentially_expressed_after_microinjection_into_sea_urchin_zygotes">Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes</a></div><div class="wp-workCard_item"><span>Proceedings of the National Academy of Sciences</span><span>, 1988</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linke...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linked in a single plasmid and injected into the eggs of the sea urchin Lytechinus pictus. The levels of transcripts of injected early and late genes and of endogenous early genes were monitored during development by a ribonuclease protection assay. Transcripts of both the injected and endogenous early genes peaked during the blastula stage and decreased severalfold by the mesenchyme blastula stage. Transcripts of the injected late gene became detectable at the blastula stage and increased in amount subsequently, until at least the early gastrula stage, 28 hr after fertilization. Thus, the pattern of expression of the injected early and late H2B genes is similar to that of their endogenous counterparts. These results show that DNA sequences regulating the temporal pattern of early and late H2B gene expression must lie within the cloned DNA segments; i.e., within 600 base pairs of the early H2...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4227c1bec1e0a648e7e39efac3812de6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854358,"asset_id":83546059,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854358/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546059"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546059"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546059; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546059]").text(description); $(".js-view-count[data-work-id=83546059]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546059; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546059']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4227c1bec1e0a648e7e39efac3812de6" } } $('.js-work-strip[data-work-id=83546059]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546059,"title":"Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes","translated_title":"","metadata":{"abstract":"An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linked in a single plasmid and injected into the eggs of the sea urchin Lytechinus pictus. 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These results show that DNA sequences regulating the temporal pattern of early and late H2B gene expression must lie within the cloned DNA segments; i.e., within 600 base pairs of the early H2...","publisher":"Proceedings of the National Academy of Sciences","publication_date":{"day":null,"month":null,"year":1988,"errors":{}},"publication_name":"Proceedings of the National Academy of Sciences"},"translated_abstract":"An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linked in a single plasmid and injected into the eggs of the sea urchin Lytechinus pictus. The levels of transcripts of injected early and late genes and of endogenous early genes were monitored during development by a ribonuclease protection assay. Transcripts of both the injected and endogenous early genes peaked during the blastula stage and decreased severalfold by the mesenchyme blastula stage. Transcripts of the injected late gene became detectable at the blastula stage and increased in amount subsequently, until at least the early gastrula stage, 28 hr after fertilization. Thus, the pattern of expression of the injected early and late H2B genes is similar to that of their endogenous counterparts. These results show that DNA sequences regulating the temporal pattern of early and late H2B gene expression must lie within the cloned DNA segments; i.e., within 600 base pairs of the early H2...","internal_url":"https://www.academia.edu/83546059/Closely_linked_early_and_late_histone_H2B_genes_are_differentially_expressed_after_microinjection_into_sea_urchin_zygotes","translated_internal_url":"","created_at":"2022-07-22T02:43:46.486-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854358,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854358/thumbnails/1.jpg","file_name":"507.full.pdf","download_url":"https://www.academia.edu/attachments/88854358/download_file","bulk_download_file_name":"Closely_linked_early_and_late_histone_H2.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854358/507.full-libre.pdf?1658483329=\u0026response-content-disposition=attachment%3B+filename%3DClosely_linked_early_and_late_histone_H2.pdf\u0026Expires=1742176507\u0026Signature=CfUCTWk7TrqCFmY3-81AWqiWC3NaK1vxfmUoGlOGvcI4bVjWOCsBDp-3b4R-O6pA93YTMW9JcY5hIW2STNXtCrxyKN3luUiReeRJPsy3iSk9klL7-~RfXSYrjFnymgvJhLOks9lxryMwGzXlMWHNHi7V8aKphynfhXqIp0FTAyhyv60tTyh4YXEQibOcIX4orvxFP6MEXYnkPK3z0CJsFU1maa67wK14IIe67K2-YQ7EhmNcH0EJhpg94ZbqLaSycjpj7qDHsHkykBx4hKs2gIVz8s0JFuA2k-Q6liDYIUe9TvtqpHqEvkrRY4L6utlPCOVJM3slJ8u0-8RW~DWbwA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Closely_linked_early_and_late_histone_H2B_genes_are_differentially_expressed_after_microinjection_into_sea_urchin_zygotes","translated_slug":"","page_count":4,"language":"en","content_type":"Work","summary":"An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linked in a single plasmid and injected into the eggs of the sea urchin Lytechinus pictus. 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These results show that DNA sequences regulating the temporal pattern of early and late H2B gene expression must lie within the cloned DNA segments; i.e., within 600 base pairs of the early H2...","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854358,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854358/thumbnails/1.jpg","file_name":"507.full.pdf","download_url":"https://www.academia.edu/attachments/88854358/download_file","bulk_download_file_name":"Closely_linked_early_and_late_histone_H2.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854358/507.full-libre.pdf?1658483329=\u0026response-content-disposition=attachment%3B+filename%3DClosely_linked_early_and_late_histone_H2.pdf\u0026Expires=1742176507\u0026Signature=CfUCTWk7TrqCFmY3-81AWqiWC3NaK1vxfmUoGlOGvcI4bVjWOCsBDp-3b4R-O6pA93YTMW9JcY5hIW2STNXtCrxyKN3luUiReeRJPsy3iSk9klL7-~RfXSYrjFnymgvJhLOks9lxryMwGzXlMWHNHi7V8aKphynfhXqIp0FTAyhyv60tTyh4YXEQibOcIX4orvxFP6MEXYnkPK3z0CJsFU1maa67wK14IIe67K2-YQ7EhmNcH0EJhpg94ZbqLaSycjpj7qDHsHkykBx4hKs2gIVz8s0JFuA2k-Q6liDYIUe9TvtqpHqEvkrRY4L6utlPCOVJM3slJ8u0-8RW~DWbwA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":11035,"name":"Regulation","url":"https://www.academia.edu/Documents/in/Regulation"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":190363,"name":"Plasmids","url":"https://www.academia.edu/Documents/in/Plasmids"},{"id":229847,"name":"Sea Urchins","url":"https://www.academia.edu/Documents/in/Sea_Urchins"},{"id":233229,"name":"Genes","url":"https://www.academia.edu/Documents/in/Genes"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning"},{"id":317185,"name":"Histones","url":"https://www.academia.edu/Documents/in/Histones"},{"id":560354,"name":"Sea Urchin","url":"https://www.academia.edu/Documents/in/Sea_Urchin"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes"},{"id":2274872,"name":"DNA sequence","url":"https://www.academia.edu/Documents/in/DNA_sequence"},{"id":2280042,"name":"Differential expression","url":"https://www.academia.edu/Documents/in/Differential_expression"},{"id":3647178,"name":"DNA Restriction Enzymes","url":"https://www.academia.edu/Documents/in/DNA_Restriction_Enzymes"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546058"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/83546058/The_Regulation_of_Tyrosinase_Gene_Transcription"><img alt="Research paper thumbnail of The Regulation of Tyrosinase Gene Transcription" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/83546058/The_Regulation_of_Tyrosinase_Gene_Transcription">The Regulation of Tyrosinase Gene Transcription</a></div><div class="wp-workCard_item"><span>Pigment Cell Research</span><span>, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity res...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity rests in part at the level of transcriptional regulation. The 5&amp;#39; promoter regions of the human, mouse, chicken, quail, snapping turtle, and frog tyrosinase sequences have been isolated and the mechanisms regulating the activity of these sequences are beginning to be elucidated. This review provides an update on the following aspects of tyrosinase gene regulation: basal promoter elements that determine the site of transcription initiation for RNA polymerase II; the cis-acting elements and DNA-binding factors that mediate melanocyte-specific expression of the tyrosinase gene; promoter elements involved in the temporal control of tyrosinase gene expression; additional elements that may be required to achieve wild-type levels of gene expression; and specific elements that may be required for modulation of tyrosinase gene expression in response to humoral factors or external stimuli that are known to influence the amounts of melanin synthesized by fully differentiated melanocytes. The wild type expression of tyrosinase is the result of the interaction of many different factors and it is becoming evident that certain elements and factors play more than one role in this process.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546058"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546058"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546058; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546058]").text(description); $(".js-view-count[data-work-id=83546058]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546058; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546058']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=83546058]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546058,"title":"The Regulation of Tyrosinase Gene Transcription","translated_title":"","metadata":{"abstract":"Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity rests in part at the level of transcriptional regulation. The 5\u0026amp;#39; promoter regions of the human, mouse, chicken, quail, snapping turtle, and frog tyrosinase sequences have been isolated and the mechanisms regulating the activity of these sequences are beginning to be elucidated. This review provides an update on the following aspects of tyrosinase gene regulation: basal promoter elements that determine the site of transcription initiation for RNA polymerase II; the cis-acting elements and DNA-binding factors that mediate melanocyte-specific expression of the tyrosinase gene; promoter elements involved in the temporal control of tyrosinase gene expression; additional elements that may be required to achieve wild-type levels of gene expression; and specific elements that may be required for modulation of tyrosinase gene expression in response to humoral factors or external stimuli that are known to influence the amounts of melanin synthesized by fully differentiated melanocytes. The wild type expression of tyrosinase is the result of the interaction of many different factors and it is becoming evident that certain elements and factors play more than one role in this process.","publisher":"Wiley","publication_date":{"day":null,"month":null,"year":1997,"errors":{}},"publication_name":"Pigment Cell Research"},"translated_abstract":"Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity rests in part at the level of transcriptional regulation. The 5\u0026amp;#39; promoter regions of the human, mouse, chicken, quail, snapping turtle, and frog tyrosinase sequences have been isolated and the mechanisms regulating the activity of these sequences are beginning to be elucidated. This review provides an update on the following aspects of tyrosinase gene regulation: basal promoter elements that determine the site of transcription initiation for RNA polymerase II; the cis-acting elements and DNA-binding factors that mediate melanocyte-specific expression of the tyrosinase gene; promoter elements involved in the temporal control of tyrosinase gene expression; additional elements that may be required to achieve wild-type levels of gene expression; and specific elements that may be required for modulation of tyrosinase gene expression in response to humoral factors or external stimuli that are known to influence the amounts of melanin synthesized by fully differentiated melanocytes. The wild type expression of tyrosinase is the result of the interaction of many different factors and it is becoming evident that certain elements and factors play more than one role in this process.","internal_url":"https://www.academia.edu/83546058/The_Regulation_of_Tyrosinase_Gene_Transcription","translated_internal_url":"","created_at":"2022-07-22T02:43:46.356-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_Regulation_of_Tyrosinase_Gene_Transcription","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity rests in part at the level of transcriptional regulation. The 5\u0026amp;#39; promoter regions of the human, mouse, chicken, quail, snapping turtle, and frog tyrosinase sequences have been isolated and the mechanisms regulating the activity of these sequences are beginning to be elucidated. This review provides an update on the following aspects of tyrosinase gene regulation: basal promoter elements that determine the site of transcription initiation for RNA polymerase II; the cis-acting elements and DNA-binding factors that mediate melanocyte-specific expression of the tyrosinase gene; promoter elements involved in the temporal control of tyrosinase gene expression; additional elements that may be required to achieve wild-type levels of gene expression; and specific elements that may be required for modulation of tyrosinase gene expression in response to humoral factors or external stimuli that are known to influence the amounts of melanin synthesized by fully differentiated melanocytes. The wild type expression of tyrosinase is the result of the interaction of many different factors and it is becoming evident that certain elements and factors play more than one role in this process.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":23323,"name":"Transcription Factors","url":"https://www.academia.edu/Documents/in/Transcription_Factors"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":92680,"name":"cyclic AMP","url":"https://www.academia.edu/Documents/in/cyclic_AMP"},{"id":103254,"name":"Vertebrates","url":"https://www.academia.edu/Documents/in/Vertebrates"},{"id":162553,"name":"Skin","url":"https://www.academia.edu/Documents/in/Skin"},{"id":349211,"name":"Skin Pigmentation","url":"https://www.academia.edu/Documents/in/Skin_Pigmentation"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":591594,"name":"Tretinoin","url":"https://www.academia.edu/Documents/in/Tretinoin"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":924535,"name":"Quail","url":"https://www.academia.edu/Documents/in/Quail"},{"id":1293308,"name":"Glucocorticoids","url":"https://www.academia.edu/Documents/in/Glucocorticoids"},{"id":1405352,"name":"Pigment Cell","url":"https://www.academia.edu/Documents/in/Pigment_Cell"},{"id":2153835,"name":"Enzyme Induction","url":"https://www.academia.edu/Documents/in/Enzyme_Induction"},{"id":3005391,"name":"Ultraviolet Rays","url":"https://www.academia.edu/Documents/in/Ultraviolet_Rays"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":22355844,"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0749.1997.tb00474.x"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546057"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546057/Red_or_rufous_albinism_in_Southern_Africa"><img alt="Research paper thumbnail of Red or rufous albinism in Southern Africa" class="work-thumbnail" src="https://attachments.academia-assets.com/88854354/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546057/Red_or_rufous_albinism_in_Southern_Africa">Red or rufous albinism in Southern Africa</a></div><div class="wp-workCard_item"><span>Ophthalmic Paediatrics and Genetics</span><span>, 1990</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">As the Alzheimer's disease process progresses in time measurements of cognitive functioning exhib...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">As the Alzheimer's disease process progresses in time measurements of cognitive functioning exhibit nonlinearity. Multiphase models were used to quantify this nonlinearity for thirty-six well characterized individuals(∼12 observations per individual over ∼15 years in the study) by partitioning each into a healthy aging phase and a diseased phase. This enabled us to detail both the magnitude and timing that Alzheimer's disease alters different aspects of cognitive function. Estimation of these models was done using Bayesian methods. Eight different outcomes representing three areas of memory functioning(visual, verbal, working) were used to define a pattern of cognitive decline. The earliest phase change was found to be visual memory(∼6 years before diagnosis) and was followed by changes in verbal and working memory beginning roughly four years later.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cc7e9d9043c929c6a5d101012aa11519" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854354,"asset_id":83546057,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854354/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546057"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546057"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546057; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546057]").text(description); $(".js-view-count[data-work-id=83546057]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546057; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546057']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cc7e9d9043c929c6a5d101012aa11519" } } $('.js-work-strip[data-work-id=83546057]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546057,"title":"Red or rufous albinism in Southern Africa","translated_title":"","metadata":{"publisher":"Informa UK Limited","grobid_abstract":"As the Alzheimer's disease process progresses in time measurements of cognitive functioning exhibit nonlinearity. Multiphase models were used to quantify this nonlinearity for thirty-six well characterized individuals(∼12 observations per individual over ∼15 years in the study) by partitioning each into a healthy aging phase and a diseased phase. This enabled us to detail both the magnitude and timing that Alzheimer's disease alters different aspects of cognitive function. Estimation of these models was done using Bayesian methods. Eight different outcomes representing three areas of memory functioning(visual, verbal, working) were used to define a pattern of cognitive decline. The earliest phase change was found to be visual memory(∼6 years before diagnosis) and was followed by changes in verbal and working memory beginning roughly four years later.","publication_date":{"day":null,"month":null,"year":1990,"errors":{}},"publication_name":"Ophthalmic Paediatrics and Genetics","grobid_abstract_attachment_id":88854354},"translated_abstract":null,"internal_url":"https://www.academia.edu/83546057/Red_or_rufous_albinism_in_Southern_Africa","translated_internal_url":"","created_at":"2022-07-22T02:43:46.175-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854354,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854354/thumbnails/1.jpg","file_name":"langford_ku_0099m_12512_data_1.pdf","download_url":"https://www.academia.edu/attachments/88854354/download_file","bulk_download_file_name":"Red_or_rufous_albinism_in_Southern_Afric.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854354/langford_ku_0099m_12512_data_1-libre.pdf?1658483333=\u0026response-content-disposition=attachment%3B+filename%3DRed_or_rufous_albinism_in_Southern_Afric.pdf\u0026Expires=1742176507\u0026Signature=br2~ZchkgrsPVejkBVkW2tUvvkdzFHHGKiYSpP-V1TnzkvCnFRDZijq0WdEQvoJFAA2~UB7t6Pce~GBUibzVj9fe5NbZOYSaR5gsqeqHEtJuvqWhW641IL0HHeQdXG9i-N4MBsjpmXQC1Jj~N3Lz6WGOTtw-C5mR3iGZRzhyTf6TiIwBk1YoUIn9lIgXNrfa6OO4tsZ4tN6At4ryRJiFGSO0zOCIFc5bpx6a0caXJuQHVv2~aWcZKlJK9BtV9RvjR7FZRDQGyeTpfw4~RywaRIIyfuckZeRWxY2rFexecpAvCkLn6gTyY-HLxFrQcjGhEA535f5gxGWncVsfyT5wdg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Red_or_rufous_albinism_in_Southern_Africa","translated_slug":"","page_count":38,"language":"en","content_type":"Work","summary":"As the Alzheimer's disease process progresses in time measurements of cognitive functioning exhibit nonlinearity. Multiphase models were used to quantify this nonlinearity for thirty-six well characterized individuals(∼12 observations per individual over ∼15 years in the study) by partitioning each into a healthy aging phase and a diseased phase. This enabled us to detail both the magnitude and timing that Alzheimer's disease alters different aspects of cognitive function. Estimation of these models was done using Bayesian methods. Eight different outcomes representing three areas of memory functioning(visual, verbal, working) were used to define a pattern of cognitive decline. The earliest phase change was found to be visual memory(∼6 years before diagnosis) and was followed by changes in verbal and working memory beginning roughly four years later.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854354,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854354/thumbnails/1.jpg","file_name":"langford_ku_0099m_12512_data_1.pdf","download_url":"https://www.academia.edu/attachments/88854354/download_file","bulk_download_file_name":"Red_or_rufous_albinism_in_Southern_Afric.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854354/langford_ku_0099m_12512_data_1-libre.pdf?1658483333=\u0026response-content-disposition=attachment%3B+filename%3DRed_or_rufous_albinism_in_Southern_Afric.pdf\u0026Expires=1742176507\u0026Signature=br2~ZchkgrsPVejkBVkW2tUvvkdzFHHGKiYSpP-V1TnzkvCnFRDZijq0WdEQvoJFAA2~UB7t6Pce~GBUibzVj9fe5NbZOYSaR5gsqeqHEtJuvqWhW641IL0HHeQdXG9i-N4MBsjpmXQC1Jj~N3Lz6WGOTtw-C5mR3iGZRzhyTf6TiIwBk1YoUIn9lIgXNrfa6OO4tsZ4tN6At4ryRJiFGSO0zOCIFc5bpx6a0caXJuQHVv2~aWcZKlJK9BtV9RvjR7FZRDQGyeTpfw4~RywaRIIyfuckZeRWxY2rFexecpAvCkLn6gTyY-HLxFrQcjGhEA535f5gxGWncVsfyT5wdg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":13135,"name":"Southern Africa","url":"https://www.academia.edu/Documents/in/Southern_Africa"},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent"},{"id":23267,"name":"Ophthalmic","url":"https://www.academia.edu/Documents/in/Ophthalmic"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":61866,"name":"South Africa","url":"https://www.academia.edu/Documents/in/South_Africa"},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child"},{"id":289330,"name":"Prevalence","url":"https://www.academia.edu/Documents/in/Prevalence"},{"id":349211,"name":"Skin Pigmentation","url":"https://www.academia.edu/Documents/in/Skin_Pigmentation"},{"id":359001,"name":"Optometry and Ophthalmology","url":"https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology"},{"id":576640,"name":"Hair Color","url":"https://www.academia.edu/Documents/in/Hair_Color"},{"id":665851,"name":"OPHTHALMIC GENETICS","url":"https://www.academia.edu/Documents/in/OPHTHALMIC_GENETICS"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":968586,"name":"Visual Evoked Potentials","url":"https://www.academia.edu/Documents/in/Visual_Evoked_Potentials"}],"urls":[{"id":22355843,"url":"http://www.tandfonline.com/doi/pdf/10.3109/13816819009020984"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546056"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546056/Corticotropin_releasing_factor_A_possible_key_to_gut_dysfunction_in_the_critically_ill"><img alt="Research paper thumbnail of Corticotropin-releasing factor: A possible key to gut dysfunction in the critically ill" class="work-thumbnail" src="https://attachments.academia-assets.com/88854353/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546056/Corticotropin_releasing_factor_A_possible_key_to_gut_dysfunction_in_the_critically_ill">Corticotropin-releasing factor: A possible key to gut dysfunction in the critically ill</a></div><div class="wp-workCard_item"><span>Nutrition</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Critically ill patients frequently display unexplained or incompletely explained features of gast...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Critically ill patients frequently display unexplained or incompletely explained features of gastrointestinal (GI) dysfunction, including gastric stasis, ileus, and diarrhea. This makes nutrition delivery challenging, and may contribute to poor outcomes. The typical bowel dysfunction seen in severely ill patients includes retarded gastric emptying, unsynchronized intestinal motility, and intestinal hyperpermeability. These functional changes appear similar to the corticotropinreleasing factor (CRF)-mediated bowel dysfunctions associated with stress of various types and some GI disorders and diseases. CRF has been shown to be present within the GI tract and its action on CRF receptors within the gut have been shown to reduce gastric emptying, alter intestinal motility, and increase intestinal permeability. However, the precise role of CRF in the GI dysfunction in critical illness remains unclear. In this short review, we provide an update on GI dysfunction during stress and review the possible role of CRF in the aetiology of gut dysfunction. We suggest that activation of CRF signaling pathways in critical illness might be key to understanding the mechanisms underlying the gut dysfunction that impairs enteral feeding in the intensive care unit.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fe64d6ee903d5a2bf17fce1ffd01faac" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854353,"asset_id":83546056,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854353/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546056"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546056"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546056; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546056]").text(description); $(".js-view-count[data-work-id=83546056]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546056; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546056']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fe64d6ee903d5a2bf17fce1ffd01faac" } } $('.js-work-strip[data-work-id=83546056]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546056,"title":"Corticotropin-releasing factor: A possible key to gut dysfunction in the critically ill","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Critically ill patients frequently display unexplained or incompletely explained features of gastrointestinal (GI) dysfunction, including gastric stasis, ileus, and diarrhea. 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We suggest that activation of CRF signaling pathways in critical illness might be key to understanding the mechanisms underlying the gut dysfunction that impairs enteral feeding in the intensive care unit.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Nutrition","grobid_abstract_attachment_id":88854353},"translated_abstract":null,"internal_url":"https://www.academia.edu/83546056/Corticotropin_releasing_factor_A_possible_key_to_gut_dysfunction_in_the_critically_ill","translated_internal_url":"","created_at":"2022-07-22T02:43:46.076-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854353,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854353/thumbnails/1.jpg","file_name":"j.nut.2012.12.02320220722-1-uprmv6.pdf","download_url":"https://www.academia.edu/attachments/88854353/download_file","bulk_download_file_name":"Corticotropin_releasing_factor_A_possibl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854353/j.nut.2012.12.02320220722-1-uprmv6-libre.pdf?1658483330=\u0026response-content-disposition=attachment%3B+filename%3DCorticotropin_releasing_factor_A_possibl.pdf\u0026Expires=1742176507\u0026Signature=GtlaWMSZ7onn7y9auLQarJ9N60q57HHZIEvJ0NwYHi6GWSBBAFWpBdlw622M117haYeBlrcHUwt5hQ~b70ktcXQCg5oNKPSVfuY4yw~eN2SZf-0HhE4j6FbNM78FrCk~2~QDe5f4RyKTHkiUt4R0-zhw6hBtBX6xsZ5j2TiDcLAYf5qCdV-IpzekroO~X3asijGSJx-Rg2hsTcUFL8s3VwdQLS5D2WtRDcY2W3cTBkaXkLyewrQtvnZUlhvcBBo7~PRaFJ4CGFGj7gMcoFHtRrKumCG6k-cyHrq1FprWwCTdfKxPLZbwkoArCSw4~jDGhzMqHCezW8UFEeYortLm~g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Corticotropin_releasing_factor_A_possible_key_to_gut_dysfunction_in_the_critically_ill","translated_slug":"","page_count":5,"language":"en","content_type":"Work","summary":"Critically ill patients frequently display unexplained or incompletely explained features of gastrointestinal (GI) dysfunction, including gastric stasis, ileus, and diarrhea. This makes nutrition delivery challenging, and may contribute to poor outcomes. The typical bowel dysfunction seen in severely ill patients includes retarded gastric emptying, unsynchronized intestinal motility, and intestinal hyperpermeability. These functional changes appear similar to the corticotropinreleasing factor (CRF)-mediated bowel dysfunctions associated with stress of various types and some GI disorders and diseases. CRF has been shown to be present within the GI tract and its action on CRF receptors within the gut have been shown to reduce gastric emptying, alter intestinal motility, and increase intestinal permeability. However, the precise role of CRF in the GI dysfunction in critical illness remains unclear. In this short review, we provide an update on GI dysfunction during stress and review the possible role of CRF in the aetiology of gut dysfunction. We suggest that activation of CRF signaling pathways in critical illness might be key to understanding the mechanisms underlying the gut dysfunction that impairs enteral feeding in the intensive care unit.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854353,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854353/thumbnails/1.jpg","file_name":"j.nut.2012.12.02320220722-1-uprmv6.pdf","download_url":"https://www.academia.edu/attachments/88854353/download_file","bulk_download_file_name":"Corticotropin_releasing_factor_A_possibl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854353/j.nut.2012.12.02320220722-1-uprmv6-libre.pdf?1658483330=\u0026response-content-disposition=attachment%3B+filename%3DCorticotropin_releasing_factor_A_possibl.pdf\u0026Expires=1742176507\u0026Signature=GtlaWMSZ7onn7y9auLQarJ9N60q57HHZIEvJ0NwYHi6GWSBBAFWpBdlw622M117haYeBlrcHUwt5hQ~b70ktcXQCg5oNKPSVfuY4yw~eN2SZf-0HhE4j6FbNM78FrCk~2~QDe5f4RyKTHkiUt4R0-zhw6hBtBX6xsZ5j2TiDcLAYf5qCdV-IpzekroO~X3asijGSJx-Rg2hsTcUFL8s3VwdQLS5D2WtRDcY2W3cTBkaXkLyewrQtvnZUlhvcBBo7~PRaFJ4CGFGj7gMcoFHtRrKumCG6k-cyHrq1FprWwCTdfKxPLZbwkoArCSw4~jDGhzMqHCezW8UFEeYortLm~g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1907,"name":"Nutrition","url":"https://www.academia.edu/Documents/in/Nutrition"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":31276,"name":"Corticotropin Releasing Hormone","url":"https://www.academia.edu/Documents/in/Corticotropin_Releasing_Hormone"},{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction"},{"id":537759,"name":"Gastrointestinal Tract","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Tract"},{"id":722449,"name":"Physiological Stress Markers","url":"https://www.academia.edu/Documents/in/Physiological_Stress_Markers"},{"id":1548059,"name":"Critical Illness","url":"https://www.academia.edu/Documents/in/Critical_Illness"},{"id":1595009,"name":"Gastric Emptying","url":"https://www.academia.edu/Documents/in/Gastric_Emptying"},{"id":1758627,"name":"Enteral Nutrition","url":"https://www.academia.edu/Documents/in/Enteral_Nutrition"},{"id":2430299,"name":"Gastrointestinal Diseases","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Diseases"},{"id":3647007,"name":"Stress Physiological","url":"https://www.academia.edu/Documents/in/Stress_Physiological"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546055"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/83546055/Corticotropin_releasing_factor_is_present_in_intestinal_tissue_of_patients_with_gastrointestinal_dysfunction_following_shock_and_abdominal_surgery"><img alt="Research paper thumbnail of Corticotropin-releasing factor is present in intestinal tissue of patients with gastrointestinal dysfunction following shock and abdominal surgery" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/83546055/Corticotropin_releasing_factor_is_present_in_intestinal_tissue_of_patients_with_gastrointestinal_dysfunction_following_shock_and_abdominal_surgery">Corticotropin-releasing factor is present in intestinal tissue of patients with gastrointestinal dysfunction following shock and abdominal surgery</a></div><div class="wp-workCard_item"><span>Nutrition</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction, possibly causing gut dysfunction following trauma and surgery. We investigated plasma and intestinal tissue CRF levels and gut function in patients with traumatic shock or those undergoing elective abdominal surgery. In a prospective, parallel, observational study in a university hospital surgical intensive care unit (ICU), 8 shocked patients (systolic blood pressure &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;90 mmHg and/or acidosis and/or urine output &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 mL/kg/hr and/or requiring &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;2 L of intravenous fluid resuscitation) undergoing small bowel resection during emergency laparotomy following abdominal injury and 17 stable patients undergoing elective hepatobiliary surgery were included if they required postoperative ICU management. Serial plasma and intestinal CRF was measured and postoperative gastric emptying and intestinal permeability were evaluated. Plasma CRF was significantly increased in the shocked patients compared with the elective surgery patients at all times. CRF peptide was quantified in intestinal tissue at similar levels in both groups. Intestinal permeability was increased and associated with shock and resuscitation fluid volume. Gastric emptying was retarded and correlated significantly with shock but not with plasma CRF. Delayed gastric emptying in shocked patients was associated with longer ICU stay. The novel finding is the presence of CRF in the small bowel of both elective and emergency gastrointestinal surgery patients with concomitant gastrointestinal dysfunction. Circulating CRF is associated with poor gastric emptying, which prolongs ICU stay, whereas shock significantly impairs gastric emptying and gut permeability.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546055"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546055"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546055; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546055]").text(description); $(".js-view-count[data-work-id=83546055]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546055; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546055']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=83546055]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546055,"title":"Corticotropin-releasing factor is present in intestinal tissue of patients with gastrointestinal dysfunction following shock and abdominal surgery","translated_title":"","metadata":{"abstract":"Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction, possibly causing gut dysfunction following trauma and surgery. We investigated plasma and intestinal tissue CRF levels and gut function in patients with traumatic shock or those undergoing elective abdominal surgery. In a prospective, parallel, observational study in a university hospital surgical intensive care unit (ICU), 8 shocked patients (systolic blood pressure \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;90 mmHg and/or acidosis and/or urine output \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 mL/kg/hr and/or requiring \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;2 L of intravenous fluid resuscitation) undergoing small bowel resection during emergency laparotomy following abdominal injury and 17 stable patients undergoing elective hepatobiliary surgery were included if they required postoperative ICU management. Serial plasma and intestinal CRF was measured and postoperative gastric emptying and intestinal permeability were evaluated. Plasma CRF was significantly increased in the shocked patients compared with the elective surgery patients at all times. CRF peptide was quantified in intestinal tissue at similar levels in both groups. Intestinal permeability was increased and associated with shock and resuscitation fluid volume. Gastric emptying was retarded and correlated significantly with shock but not with plasma CRF. Delayed gastric emptying in shocked patients was associated with longer ICU stay. The novel finding is the presence of CRF in the small bowel of both elective and emergency gastrointestinal surgery patients with concomitant gastrointestinal dysfunction. Circulating CRF is associated with poor gastric emptying, which prolongs ICU stay, whereas shock significantly impairs gastric emptying and gut permeability.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Nutrition"},"translated_abstract":"Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction, possibly causing gut dysfunction following trauma and surgery. We investigated plasma and intestinal tissue CRF levels and gut function in patients with traumatic shock or those undergoing elective abdominal surgery. In a prospective, parallel, observational study in a university hospital surgical intensive care unit (ICU), 8 shocked patients (systolic blood pressure \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;90 mmHg and/or acidosis and/or urine output \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 mL/kg/hr and/or requiring \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;2 L of intravenous fluid resuscitation) undergoing small bowel resection during emergency laparotomy following abdominal injury and 17 stable patients undergoing elective hepatobiliary surgery were included if they required postoperative ICU management. Serial plasma and intestinal CRF was measured and postoperative gastric emptying and intestinal permeability were evaluated. Plasma CRF was significantly increased in the shocked patients compared with the elective surgery patients at all times. CRF peptide was quantified in intestinal tissue at similar levels in both groups. Intestinal permeability was increased and associated with shock and resuscitation fluid volume. Gastric emptying was retarded and correlated significantly with shock but not with plasma CRF. Delayed gastric emptying in shocked patients was associated with longer ICU stay. The novel finding is the presence of CRF in the small bowel of both elective and emergency gastrointestinal surgery patients with concomitant gastrointestinal dysfunction. Circulating CRF is associated with poor gastric emptying, which prolongs ICU stay, whereas shock significantly impairs gastric emptying and gut permeability.","internal_url":"https://www.academia.edu/83546055/Corticotropin_releasing_factor_is_present_in_intestinal_tissue_of_patients_with_gastrointestinal_dysfunction_following_shock_and_abdominal_surgery","translated_internal_url":"","created_at":"2022-07-22T02:43:45.773-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Corticotropin_releasing_factor_is_present_in_intestinal_tissue_of_patients_with_gastrointestinal_dysfunction_following_shock_and_abdominal_surgery","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction, possibly causing gut dysfunction following trauma and surgery. We investigated plasma and intestinal tissue CRF levels and gut function in patients with traumatic shock or those undergoing elective abdominal surgery. In a prospective, parallel, observational study in a university hospital surgical intensive care unit (ICU), 8 shocked patients (systolic blood pressure \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;90 mmHg and/or acidosis and/or urine output \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 mL/kg/hr and/or requiring \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;2 L of intravenous fluid resuscitation) undergoing small bowel resection during emergency laparotomy following abdominal injury and 17 stable patients undergoing elective hepatobiliary surgery were included if they required postoperative ICU management. Serial plasma and intestinal CRF was measured and postoperative gastric emptying and intestinal permeability were evaluated. Plasma CRF was significantly increased in the shocked patients compared with the elective surgery patients at all times. CRF peptide was quantified in intestinal tissue at similar levels in both groups. Intestinal permeability was increased and associated with shock and resuscitation fluid volume. Gastric emptying was retarded and correlated significantly with shock but not with plasma CRF. Delayed gastric emptying in shocked patients was associated with longer ICU stay. The novel finding is the presence of CRF in the small bowel of both elective and emergency gastrointestinal surgery patients with concomitant gastrointestinal dysfunction. Circulating CRF is associated with poor gastric emptying, which prolongs ICU stay, whereas shock significantly impairs gastric emptying and gut permeability.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[],"research_interests":[{"id":1907,"name":"Nutrition","url":"https://www.academia.edu/Documents/in/Nutrition"},{"id":31276,"name":"Corticotropin Releasing Hormone","url":"https://www.academia.edu/Documents/in/Corticotropin_Releasing_Hormone"},{"id":55155,"name":"Emergency Medical Services","url":"https://www.academia.edu/Documents/in/Emergency_Medical_Services"},{"id":211917,"name":"Length of Stay","url":"https://www.academia.edu/Documents/in/Length_of_Stay"},{"id":288383,"name":"Intestinal absorption","url":"https://www.academia.edu/Documents/in/Intestinal_absorption"},{"id":291136,"name":"Intestines","url":"https://www.academia.edu/Documents/in/Intestines"},{"id":550383,"name":"Gastrointestinal motility","url":"https://www.academia.edu/Documents/in/Gastrointestinal_motility"},{"id":647820,"name":"Fluid Therapy","url":"https://www.academia.edu/Documents/in/Fluid_Therapy"},{"id":897823,"name":"Elsevier","url":"https://www.academia.edu/Documents/in/Elsevier"},{"id":1595009,"name":"Gastric Emptying","url":"https://www.academia.edu/Documents/in/Gastric_Emptying"},{"id":1653983,"name":"Abdomen","url":"https://www.academia.edu/Documents/in/Abdomen"},{"id":2430299,"name":"Gastrointestinal Diseases","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Diseases"},{"id":2463621,"name":"Postoperative Complications","url":"https://www.academia.edu/Documents/in/Postoperative_Complications"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"},{"id":3834135,"name":"abdominal Injuries","url":"https://www.academia.edu/Documents/in/abdominal_Injuries"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="14729973" id="papers"><div class="js-work-strip profile--work_container" data-work-id="115467566"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/115467566/Properties_and_Functions_of_the_Vessels_of_the_Ciliary_Body"><img alt="Research paper thumbnail of Properties and Functions of the Vessels of the Ciliary Body" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/115467566/Properties_and_Functions_of_the_Vessels_of_the_Ciliary_Body">Properties and Functions of the Vessels of the Ciliary Body</a></div><div class="wp-workCard_item"><span>Encyclopedia of the Eye</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from the four recti muscle arteries) and the long posterior ciliary arteries which traverse the interior. A complex arrangement of branches and anastomoses from these cells culminates circularly arranged vessels, including the episcleral arterial circle, the intramuscular circle of the ciliary muscle, and the major arterial circle (MAC). Each ciliary process receives blood from branches off the MAC, which follow a tortuous route through three vascular territories. Venules drain through the pars plana and exit via the vortex veins. CB vessels have wide lumens and are fenestrated, allowing production of aqueous humor. The complexity, density, and redundancy of vessels ensures consistent perfusion of the anterior segment.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="115467566"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="115467566"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 115467566; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=115467566]").text(description); $(".js-view-count[data-work-id=115467566]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 115467566; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='115467566']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=115467566]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":115467566,"title":"Properties and Functions of the Vessels of the Ciliary Body","translated_title":"","metadata":{"abstract":"The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from the four recti muscle arteries) and the long posterior ciliary arteries which traverse the interior. A complex arrangement of branches and anastomoses from these cells culminates circularly arranged vessels, including the episcleral arterial circle, the intramuscular circle of the ciliary muscle, and the major arterial circle (MAC). Each ciliary process receives blood from branches off the MAC, which follow a tortuous route through three vascular territories. Venules drain through the pars plana and exit via the vortex veins. CB vessels have wide lumens and are fenestrated, allowing production of aqueous humor. The complexity, density, and redundancy of vessels ensures consistent perfusion of the anterior segment.","publisher":"Elsevier","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Encyclopedia of the Eye"},"translated_abstract":"The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from the four recti muscle arteries) and the long posterior ciliary arteries which traverse the interior. A complex arrangement of branches and anastomoses from these cells culminates circularly arranged vessels, including the episcleral arterial circle, the intramuscular circle of the ciliary muscle, and the major arterial circle (MAC). Each ciliary process receives blood from branches off the MAC, which follow a tortuous route through three vascular territories. Venules drain through the pars plana and exit via the vortex veins. CB vessels have wide lumens and are fenestrated, allowing production of aqueous humor. The complexity, density, and redundancy of vessels ensures consistent perfusion of the anterior segment.","internal_url":"https://www.academia.edu/115467566/Properties_and_Functions_of_the_Vessels_of_the_Ciliary_Body","translated_internal_url":"","created_at":"2024-02-26T15:01:25.040-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Properties_and_Functions_of_the_Vessels_of_the_Ciliary_Body","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The ciliary body (CB) is supplied with blood from the anterior ciliary arteries (which arise from the four recti muscle arteries) and the long posterior ciliary arteries which traverse the interior. A complex arrangement of branches and anastomoses from these cells culminates circularly arranged vessels, including the episcleral arterial circle, the intramuscular circle of the ciliary muscle, and the major arterial circle (MAC). Each ciliary process receives blood from branches off the MAC, which follow a tortuous route through three vascular territories. Venules drain through the pars plana and exit via the vortex veins. CB vessels have wide lumens and are fenestrated, allowing production of aqueous humor. The complexity, density, and redundancy of vessels ensures consistent perfusion of the anterior segment.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":54589,"name":"Anatomy","url":"https://www.academia.edu/Documents/in/Anatomy"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="115467565"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/115467565/Wholemount_imaging_reveals_abnormalities_of_the_aqueous_outflow_pathway_and_corneal_vascularity_in_Foxc1_and_Bmp4_heterozygous_mice"><img alt="Research paper thumbnail of Wholemount imaging reveals abnormalities of the aqueous outflow pathway and corneal vascularity in Foxc1 and Bmp4 heterozygous mice" class="work-thumbnail" src="https://attachments.academia-assets.com/111866192/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/115467565/Wholemount_imaging_reveals_abnormalities_of_the_aqueous_outflow_pathway_and_corneal_vascularity_in_Foxc1_and_Bmp4_heterozygous_mice">Wholemount imaging reveals abnormalities of the aqueous outflow pathway and corneal vascularity in Foxc1 and Bmp4 heterozygous mice</a></div><div class="wp-workCard_item"><span>Experimental eye research</span><span>, Jan 9, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congeni...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congenital anterior segment dysgenesis (ASD) and the development of the aqueous outflow structures throughout the limbus. The aim of this study was to advance our understanding of anterior segment abnormalities in mouse models of ASD using a 3-D imaging approach. Holistic imaging information combined with quantitative measurements were carried out on PECAM-1 stained individual components of the aqueous outflow vessels and corneal vasculature of Foxc1(+/-) on the C57BL/6Jx129 and ICR backgrounds, Bmp4(+/-) ICR mice, and wildtype mice from each background. In both wildtype and heterozygotes, singular, bifurcated and plexus forms of Schlemm&#39;s canal were noted. Of note, missing portions of the canal were seen in the heterozygous groups but not in wildtype animals. In general, we found the number of collector channels to be reduced in both heterozygotes. Lastly, we found a significant increase i...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8e17f0abf58003777964770c09151494" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":111866192,"asset_id":115467565,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/111866192/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="115467565"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="115467565"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 115467565; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=115467565]").text(description); $(".js-view-count[data-work-id=115467565]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 115467565; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='115467565']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8e17f0abf58003777964770c09151494" } } $('.js-work-strip[data-work-id=115467565]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":115467565,"title":"Wholemount imaging reveals abnormalities of the aqueous outflow pathway and corneal vascularity in Foxc1 and Bmp4 heterozygous mice","translated_title":"","metadata":{"abstract":"Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congenital anterior segment dysgenesis (ASD) and the development of the aqueous outflow structures throughout the limbus. The aim of this study was to advance our understanding of anterior segment abnormalities in mouse models of ASD using a 3-D imaging approach. Holistic imaging information combined with quantitative measurements were carried out on PECAM-1 stained individual components of the aqueous outflow vessels and corneal vasculature of Foxc1(+/-) on the C57BL/6Jx129 and ICR backgrounds, Bmp4(+/-) ICR mice, and wildtype mice from each background. In both wildtype and heterozygotes, singular, bifurcated and plexus forms of Schlemm\u0026#39;s canal were noted. Of note, missing portions of the canal were seen in the heterozygous groups but not in wildtype animals. In general, we found the number of collector channels to be reduced in both heterozygotes. Lastly, we found a significant increase i...","ai_title_tag":"Corneal and Aqueous Pathway Abnormalities in Mice","publication_date":{"day":9,"month":1,"year":2016,"errors":{}},"publication_name":"Experimental eye research"},"translated_abstract":"Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congenital anterior segment dysgenesis (ASD) and the development of the aqueous outflow structures throughout the limbus. The aim of this study was to advance our understanding of anterior segment abnormalities in mouse models of ASD using a 3-D imaging approach. Holistic imaging information combined with quantitative measurements were carried out on PECAM-1 stained individual components of the aqueous outflow vessels and corneal vasculature of Foxc1(+/-) on the C57BL/6Jx129 and ICR backgrounds, Bmp4(+/-) ICR mice, and wildtype mice from each background. In both wildtype and heterozygotes, singular, bifurcated and plexus forms of Schlemm\u0026#39;s canal were noted. Of note, missing portions of the canal were seen in the heterozygous groups but not in wildtype animals. In general, we found the number of collector channels to be reduced in both heterozygotes. Lastly, we found a significant increase i...","internal_url":"https://www.academia.edu/115467565/Wholemount_imaging_reveals_abnormalities_of_the_aqueous_outflow_pathway_and_corneal_vascularity_in_Foxc1_and_Bmp4_heterozygous_mice","translated_internal_url":"","created_at":"2024-02-26T15:01:24.779-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":111866192,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/111866192/thumbnails/1.jpg","file_name":"j.exer.2016.04.00320240226-1-lkt20n.pdf","download_url":"https://www.academia.edu/attachments/111866192/download_file","bulk_download_file_name":"Wholemount_imaging_reveals_abnormalities.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/111866192/j.exer.2016.04.00320240226-1-lkt20n-libre.pdf?1708990844=\u0026response-content-disposition=attachment%3B+filename%3DWholemount_imaging_reveals_abnormalities.pdf\u0026Expires=1742176506\u0026Signature=gMOQrCII7eiHVCNLw4wrGNFlbUlrNwpxgv09c00lfsUOtW5iOQ1h~jkVht8yyJz8r6SnPjoBpgSsIC8DHdf~fef0UnGOzWjty~0zHMNx0r3gsLrInbbQpEyXP236vZWf28Y4bWEXPTWeb~BsIviCtUmJWTbjwmqgyNCsEa~lfUbMDS~Dr4CZy129q16XCRED~genoq-T415uEwD4ARneY768v7-p8HNqGqFnpYvS3qxMH~F3lSuNdhNIvpqSG7JAhM59wbSSlVzcNH2aQ6sBaXYcggssal7FXI9rIW788UXApvUmeERBYADlrHfGWbvB5pOuDziC85kfIQ0gRPK1fA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Wholemount_imaging_reveals_abnormalities_of_the_aqueous_outflow_pathway_and_corneal_vascularity_in_Foxc1_and_Bmp4_heterozygous_mice","translated_slug":"","page_count":11,"language":"en","content_type":"Work","summary":"Mutations in the FOXC1/Foxc1 gene in humans and mice and Bmp4 in mice are associated with congenital anterior segment dysgenesis (ASD) and the development of the aqueous outflow structures throughout the limbus. The aim of this study was to advance our understanding of anterior segment abnormalities in mouse models of ASD using a 3-D imaging approach. Holistic imaging information combined with quantitative measurements were carried out on PECAM-1 stained individual components of the aqueous outflow vessels and corneal vasculature of Foxc1(+/-) on the C57BL/6Jx129 and ICR backgrounds, Bmp4(+/-) ICR mice, and wildtype mice from each background. In both wildtype and heterozygotes, singular, bifurcated and plexus forms of Schlemm\u0026#39;s canal were noted. Of note, missing portions of the canal were seen in the heterozygous groups but not in wildtype animals. In general, we found the number of collector channels to be reduced in both heterozygotes. Lastly, we found a significant increase i...","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":111866192,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/111866192/thumbnails/1.jpg","file_name":"j.exer.2016.04.00320240226-1-lkt20n.pdf","download_url":"https://www.academia.edu/attachments/111866192/download_file","bulk_download_file_name":"Wholemount_imaging_reveals_abnormalities.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/111866192/j.exer.2016.04.00320240226-1-lkt20n-libre.pdf?1708990844=\u0026response-content-disposition=attachment%3B+filename%3DWholemount_imaging_reveals_abnormalities.pdf\u0026Expires=1742176506\u0026Signature=gMOQrCII7eiHVCNLw4wrGNFlbUlrNwpxgv09c00lfsUOtW5iOQ1h~jkVht8yyJz8r6SnPjoBpgSsIC8DHdf~fef0UnGOzWjty~0zHMNx0r3gsLrInbbQpEyXP236vZWf28Y4bWEXPTWeb~BsIviCtUmJWTbjwmqgyNCsEa~lfUbMDS~Dr4CZy129q16XCRED~genoq-T415uEwD4ARneY768v7-p8HNqGqFnpYvS3qxMH~F3lSuNdhNIvpqSG7JAhM59wbSSlVzcNH2aQ6sBaXYcggssal7FXI9rIW788UXApvUmeERBYADlrHfGWbvB5pOuDziC85kfIQ0gRPK1fA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":227709,"name":"Outflow","url":"https://www.academia.edu/Documents/in/Outflow"},{"id":359001,"name":"Optometry and Ophthalmology","url":"https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology"},{"id":1198614,"name":"Vascularity","url":"https://www.academia.edu/Documents/in/Vascularity"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences"},{"id":3789880,"name":"Medical biochemistry and metabolomics","url":"https://www.academia.edu/Documents/in/Medical_biochemistry_and_metabolomics"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="115467563"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/115467563/Postnatal_development_of_the_eye_in_the_naked_mole_rat_Heterocephalus_glaber_"><img alt="Research paper thumbnail of Postnatal development of the eye in the naked mole rat (Heterocephalus glaber)" class="work-thumbnail" src="https://attachments.academia-assets.com/111866191/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/115467563/Postnatal_development_of_the_eye_in_the_naked_mole_rat_Heterocephalus_glaber_">Postnatal development of the eye in the naked mole rat (Heterocephalus glaber)</a></div><div class="wp-workCard_item"><span>The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology</span><span>, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be visually nonfunctional and as such is an ideal animal with which to pursue questions in evolutionary developmental biology. This report is the first in-depth study on the development and morphology of the naked mole rat eye. Using standard histological analysis and scanning and transmission electron microscopy, we describe the structural features of the eye. We further report on the morphological changes that accompany the development of this eye from neonate to adult and compare them with those that occur during mouse eye development. We observed numerous abnormalities in the shape and cellular arrangement of the structures of the anterior chamber, with notable malformations of the lens. Cell proliferation and cell death assays were conducted to investigate the possible causes of lens malformation. We found that neither of these processes appeared abnormal, indicating that they were not...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9ab3e0fe54d649d02861642d45edb995" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":111866191,"asset_id":115467563,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/111866191/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="115467563"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="115467563"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 115467563; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=115467563]").text(description); $(".js-view-count[data-work-id=115467563]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 115467563; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='115467563']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9ab3e0fe54d649d02861642d45edb995" } } $('.js-work-strip[data-work-id=115467563]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":115467563,"title":"Postnatal development of the eye in the naked mole rat (Heterocephalus glaber)","translated_title":"","metadata":{"abstract":"The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be visually nonfunctional and as such is an ideal animal with which to pursue questions in evolutionary developmental biology. This report is the first in-depth study on the development and morphology of the naked mole rat eye. Using standard histological analysis and scanning and transmission electron microscopy, we describe the structural features of the eye. We further report on the morphological changes that accompany the development of this eye from neonate to adult and compare them with those that occur during mouse eye development. We observed numerous abnormalities in the shape and cellular arrangement of the structures of the anterior chamber, with notable malformations of the lens. Cell proliferation and cell death assays were conducted to investigate the possible causes of lens malformation. We found that neither of these processes appeared abnormal, indicating that they were not...","publication_date":{"day":null,"month":null,"year":2004,"errors":{}},"publication_name":"The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology"},"translated_abstract":"The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be visually nonfunctional and as such is an ideal animal with which to pursue questions in evolutionary developmental biology. This report is the first in-depth study on the development and morphology of the naked mole rat eye. Using standard histological analysis and scanning and transmission electron microscopy, we describe the structural features of the eye. We further report on the morphological changes that accompany the development of this eye from neonate to adult and compare them with those that occur during mouse eye development. We observed numerous abnormalities in the shape and cellular arrangement of the structures of the anterior chamber, with notable malformations of the lens. Cell proliferation and cell death assays were conducted to investigate the possible causes of lens malformation. We found that neither of these processes appeared abnormal, indicating that they were not...","internal_url":"https://www.academia.edu/115467563/Postnatal_development_of_the_eye_in_the_naked_mole_rat_Heterocephalus_glaber_","translated_internal_url":"","created_at":"2024-02-26T15:01:09.111-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":111866191,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/111866191/thumbnails/1.jpg","file_name":"ar.a.2002520240226-1-ln9civ.pdf","download_url":"https://www.academia.edu/attachments/111866191/download_file","bulk_download_file_name":"Postnatal_development_of_the_eye_in_the.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/111866191/ar.a.2002520240226-1-ln9civ-libre.pdf?1708990846=\u0026response-content-disposition=attachment%3B+filename%3DPostnatal_development_of_the_eye_in_the.pdf\u0026Expires=1742176506\u0026Signature=NYz4ozeJ37jntqDn8xszXzlH4Q90IPA3p4zE1JK59qf6GSgj520J8~maeZej3fRN5UOlEP6RamrNL22Z~T3Glswv0JtY~id9tH2skMyBZYBAJcZaRLdQODe8b43m1Q9ShOgoik37zUKoohPa81bReYlL5RsTNtc~Fb9oszshgiNLYbbIlgM5UsP8Bpo51xIPox~~VCLDgHG899f0NhcojLemtl~~P1fNH3PW29~lfCl8rlf3BScTUoqlQHzqoHpbgk00DO19CKuq95auW5~43jXQi-tEBqDh5Bll6HcaUKZnc0mKA1pbQgMx1UwOlx0dJdl4HxHKxJdTwhSfEpjxjg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Postnatal_development_of_the_eye_in_the_naked_mole_rat_Heterocephalus_glaber_","translated_slug":"","page_count":21,"language":"en","content_type":"Work","summary":"The naked mole rat (Heterocephalus glaber) is a subterranean rodent whose eyes are thought to be visually nonfunctional and as such is an ideal animal with which to pursue questions in evolutionary developmental biology. This report is the first in-depth study on the development and morphology of the naked mole rat eye. Using standard histological analysis and scanning and transmission electron microscopy, we describe the structural features of the eye. We further report on the morphological changes that accompany the development of this eye from neonate to adult and compare them with those that occur during mouse eye development. We observed numerous abnormalities in the shape and cellular arrangement of the structures of the anterior chamber, with notable malformations of the lens. Cell proliferation and cell death assays were conducted to investigate the possible causes of lens malformation. We found that neither of these processes appeared abnormal, indicating that they were not...","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":111866191,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/111866191/thumbnails/1.jpg","file_name":"ar.a.2002520240226-1-ln9civ.pdf","download_url":"https://www.academia.edu/attachments/111866191/download_file","bulk_download_file_name":"Postnatal_development_of_the_eye_in_the.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/111866191/ar.a.2002520240226-1-ln9civ-libre.pdf?1708990846=\u0026response-content-disposition=attachment%3B+filename%3DPostnatal_development_of_the_eye_in_the.pdf\u0026Expires=1742176506\u0026Signature=NYz4ozeJ37jntqDn8xszXzlH4Q90IPA3p4zE1JK59qf6GSgj520J8~maeZej3fRN5UOlEP6RamrNL22Z~T3Glswv0JtY~id9tH2skMyBZYBAJcZaRLdQODe8b43m1Q9ShOgoik37zUKoohPa81bReYlL5RsTNtc~Fb9oszshgiNLYbbIlgM5UsP8Bpo51xIPox~~VCLDgHG899f0NhcojLemtl~~P1fNH3PW29~lfCl8rlf3BScTUoqlQHzqoHpbgk00DO19CKuq95auW5~43jXQi-tEBqDh5Bll6HcaUKZnc0mKA1pbQgMx1UwOlx0dJdl4HxHKxJdTwhSfEpjxjg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":24124,"name":"Morphogenesis","url":"https://www.academia.edu/Documents/in/Morphogenesis"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":37895,"name":"Immunocytochemistry","url":"https://www.academia.edu/Documents/in/Immunocytochemistry"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":115226,"name":"Eye Development","url":"https://www.academia.edu/Documents/in/Eye_Development"},{"id":245634,"name":"Eye","url":"https://www.academia.edu/Documents/in/Eye"},{"id":401978,"name":"Crystallin","url":"https://www.academia.edu/Documents/in/Crystallin"},{"id":408559,"name":"Mole","url":"https://www.academia.edu/Documents/in/Mole"},{"id":510714,"name":"Postnatal development","url":"https://www.academia.edu/Documents/in/Postnatal_development"},{"id":1463425,"name":"Mole-Rats","url":"https://www.academia.edu/Documents/in/Mole-Rats"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="109411763"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/109411763/Molecular_cloning_and_sequence_analysis_of_a_chicken_cDNA_encoding_tyrosinase_related_protein_2_DOPAchrome_tautomerase"><img alt="Research paper thumbnail of Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase" class="work-thumbnail" src="https://attachments.academia-assets.com/107544899/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/109411763/Molecular_cloning_and_sequence_analysis_of_a_chicken_cDNA_encoding_tyrosinase_related_protein_2_DOPAchrome_tautomerase">Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase</a></div><div class="wp-workCard_item"><span>Gene</span><span>, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have cloned and sequenced a chicken cDNA encoding an -DOPAchrome tautomerase (DCT) from an em...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have cloned and sequenced a chicken cDNA encoding an -DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a DCT transcript of 3.5 kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken DCT gene consists of several introns and spans between 15 and 30 kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="056dd8cf6d27385405d9fc35d997277a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":107544899,"asset_id":109411763,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/107544899/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="109411763"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="109411763"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 109411763; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=109411763]").text(description); $(".js-view-count[data-work-id=109411763]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 109411763; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='109411763']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "056dd8cf6d27385405d9fc35d997277a" } } $('.js-work-strip[data-work-id=109411763]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":109411763,"title":"Molecular cloning and sequence analysis of a chicken cDNA encoding tyrosinase-related protein-2/DOPAchrome tautomerase","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"We have cloned and sequenced a chicken cDNA encoding an -DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a DCT transcript of 3.5 kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken DCT gene consists of several introns and spans between 15 and 30 kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.","publication_date":{"day":null,"month":null,"year":1998,"errors":{}},"publication_name":"Gene","grobid_abstract_attachment_id":107544899},"translated_abstract":null,"internal_url":"https://www.academia.edu/109411763/Molecular_cloning_and_sequence_analysis_of_a_chicken_cDNA_encoding_tyrosinase_related_protein_2_DOPAchrome_tautomerase","translated_internal_url":"","created_at":"2023-11-19T14:06:08.484-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":107544899,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/107544899/thumbnails/1.jpg","file_name":"350b3b78c420f6cd8bfa2ab10d011a9b9422.pdf","download_url":"https://www.academia.edu/attachments/107544899/download_file","bulk_download_file_name":"Molecular_cloning_and_sequence_analysis.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/107544899/350b3b78c420f6cd8bfa2ab10d011a9b9422-libre.pdf?1700434318=\u0026response-content-disposition=attachment%3B+filename%3DMolecular_cloning_and_sequence_analysis.pdf\u0026Expires=1742176506\u0026Signature=FdJP8HGehgzSMbqSW84oIDk57KotoDwq-QzxCUC-a-knPAINDGV0AzqyWQmFOvePW04uIzNOwerfcW1zkgKuLxv8kb8oEsZlseOKAOwJOYeqEoXCgxV9mt7wGYNrFgMfIIMYauXV-y74V-mGI0ioB6AtEFfiEnqNK46HMtfZPgd6m3DW5Y2v1ECrv70i0A5YrNsoAbFyhTe3Hq5IF256xLxRwlh4UvNlPpxKGGwWEHnNa5XjAMsmorrk42UhnPV5mWpMRuSvBoswB6d60LG~pJLUx1YQgmLhxp8MdI5C7atGlGWmtMotuX2dgFS26K~zpkShn0-h9NiVIpXaCZJtCQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Molecular_cloning_and_sequence_analysis_of_a_chicken_cDNA_encoding_tyrosinase_related_protein_2_DOPAchrome_tautomerase","translated_slug":"","page_count":9,"language":"en","content_type":"Work","summary":"We have cloned and sequenced a chicken cDNA encoding an -DOPAchrome tautomerase (DCT) from an embryonic melanocyte cDNA library. The chicken DCT gene encodes a deduced protein of 516 amino acids (aas) and shares 69.2% and 69.9% aa sequence identity with the deduced mouse and human DCT proteins, respectively. Northern blot hybridisation analysis reveals a DCT transcript of 3.5 kb in RNA from the retinal pigment epithelium (RPE) of chick embryos. Genomic Southern blot hybridisation analysis suggests that the chicken DCT gene consists of several introns and spans between 15 and 30 kb of the chicken genome. This study completes the sequencing of all the members of the chicken tyrosinase-related protein gene family and provides evidence that this gene family is conserved between avians and mammals.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":107544899,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/107544899/thumbnails/1.jpg","file_name":"350b3b78c420f6cd8bfa2ab10d011a9b9422.pdf","download_url":"https://www.academia.edu/attachments/107544899/download_file","bulk_download_file_name":"Molecular_cloning_and_sequence_analysis.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/107544899/350b3b78c420f6cd8bfa2ab10d011a9b9422-libre.pdf?1700434318=\u0026response-content-disposition=attachment%3B+filename%3DMolecular_cloning_and_sequence_analysis.pdf\u0026Expires=1742176506\u0026Signature=FdJP8HGehgzSMbqSW84oIDk57KotoDwq-QzxCUC-a-knPAINDGV0AzqyWQmFOvePW04uIzNOwerfcW1zkgKuLxv8kb8oEsZlseOKAOwJOYeqEoXCgxV9mt7wGYNrFgMfIIMYauXV-y74V-mGI0ioB6AtEFfiEnqNK46HMtfZPgd6m3DW5Y2v1ECrv70i0A5YrNsoAbFyhTe3Hq5IF256xLxRwlh4UvNlPpxKGGwWEHnNa5XjAMsmorrk42UhnPV5mWpMRuSvBoswB6d60LG~pJLUx1YQgmLhxp8MdI5C7atGlGWmtMotuX2dgFS26K~zpkShn0-h9NiVIpXaCZJtCQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning"},{"id":329263,"name":"Epidermal Growth Factor","url":"https://www.academia.edu/Documents/in/Epidermal_Growth_Factor"},{"id":371424,"name":"Genomic DNA","url":"https://www.academia.edu/Documents/in/Genomic_DNA"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":569436,"name":"Genomic Library","url":"https://www.academia.edu/Documents/in/Genomic_Library"},{"id":602609,"name":"Intron","url":"https://www.academia.edu/Documents/in/Intron"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":999803,"name":"Gene Family","url":"https://www.academia.edu/Documents/in/Gene_Family"},{"id":1578793,"name":"cDNA library","url":"https://www.academia.edu/Documents/in/cDNA_library"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"},{"id":3675689,"name":"Chick embryo","url":"https://www.academia.edu/Documents/in/Chick_embryo"}],"urls":[{"id":35559960,"url":"https://api.elsevier.com/content/article/PII:S037811199800403X?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="109411761"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/109411761/The_cloning_and_sequencing_of_a_cDNA_coding_for_chick_tyrosinase_related_protein_1"><img alt="Research paper thumbnail of The cloning and sequencing of a cDNA coding for chick tyrosinase-related protein-1" class="work-thumbnail" src="https://attachments.academia-assets.com/107544889/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/109411761/The_cloning_and_sequencing_of_a_cDNA_coding_for_chick_tyrosinase_related_protein_1">The cloning and sequencing of a cDNA coding for chick tyrosinase-related protein-1</a></div><div class="wp-workCard_item"><span>Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression</span><span>, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have cloned a cDNA encoding an avian homologue of the mammalian brownr TYRP1 locus protein. Th...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have cloned a cDNA encoding an avian homologue of the mammalian brownr TYRP1 locus protein. The chick Ž. tyrosinase-related protein-1 TRP-1 gene encodes a deduced protein of 535 amino acids, shares) 65% amino acid sequence identity with fish and mammalian TRP-1 proteins, and spans 5-11 kb of the chick genome. q 1998 Elsevier Science B.V.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="053bf98b3f4b1533506c81f367add8cf" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":107544889,"asset_id":109411761,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/107544889/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="109411761"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="109411761"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 109411761; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=109411761]").text(description); $(".js-view-count[data-work-id=109411761]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 109411761; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='109411761']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "053bf98b3f4b1533506c81f367add8cf" } } $('.js-work-strip[data-work-id=109411761]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":109411761,"title":"The cloning and sequencing of a cDNA coding for chick tyrosinase-related protein-1","translated_title":"","metadata":{"publisher":"Elsevier BV","ai_title_tag":"Cloning and Sequencing of Chick TRP-1 cDNA","grobid_abstract":"We have cloned a cDNA encoding an avian homologue of the mammalian brownr TYRP1 locus protein. The chick Ž. tyrosinase-related protein-1 TRP-1 gene encodes a deduced protein of 535 amino acids, shares) 65% amino acid sequence identity with fish and mammalian TRP-1 proteins, and spans 5-11 kb of the chick genome. q 1998 Elsevier Science B.V.","publication_date":{"day":null,"month":null,"year":1998,"errors":{}},"publication_name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","grobid_abstract_attachment_id":107544889},"translated_abstract":null,"internal_url":"https://www.academia.edu/109411761/The_cloning_and_sequencing_of_a_cDNA_coding_for_chick_tyrosinase_related_protein_1","translated_internal_url":"","created_at":"2023-11-19T14:06:07.292-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":107544889,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/107544889/thumbnails/1.jpg","file_name":"s0167-478128972900144-920231119-1-lj9rcq.pdf","download_url":"https://www.academia.edu/attachments/107544889/download_file","bulk_download_file_name":"The_cloning_and_sequencing_of_a_cDNA_cod.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/107544889/s0167-478128972900144-920231119-1-lj9rcq-libre.pdf?1700434315=\u0026response-content-disposition=attachment%3B+filename%3DThe_cloning_and_sequencing_of_a_cDNA_cod.pdf\u0026Expires=1742176506\u0026Signature=KqM8o1OnfiEbMx8EKY2gZu2pZTFj~rOz9PPSv11pD2bOKnIf25DGsDv965wDMNqolTAOKlPtPpurkD7eTC30FNu7XUadiMpPRSbMaq4Ag0BsZDRAvSk833Oh76rNuVw8WO2E~4SE8CVp-AcdN~o5zroQbW0GPYj4Y7dVHlrZwND6PNRuPCmVqCprRBdOV7KPBGaXOTWqYGA4Leycp4mv8veKaJ2ZmaBhMXeK~cSQQ1xIBfzm7zcMRhbvVAt4yMtugy6tbXgIx~tZSMYdl0271SIeT~G8OKQd7lYQZrl4SS8EeTdUB8dLN5-Gwjo~PZHRLSNTpNgt5Eez0E9HuA7LZg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_cloning_and_sequencing_of_a_cDNA_coding_for_chick_tyrosinase_related_protein_1","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"We have cloned a cDNA encoding an avian homologue of the mammalian brownr TYRP1 locus protein. The chick Ž. tyrosinase-related protein-1 TRP-1 gene encodes a deduced protein of 535 amino acids, shares) 65% amino acid sequence identity with fish and mammalian TRP-1 proteins, and spans 5-11 kb of the chick genome. q 1998 Elsevier Science B.V.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":107544889,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/107544889/thumbnails/1.jpg","file_name":"s0167-478128972900144-920231119-1-lj9rcq.pdf","download_url":"https://www.academia.edu/attachments/107544889/download_file","bulk_download_file_name":"The_cloning_and_sequencing_of_a_cDNA_cod.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/107544889/s0167-478128972900144-920231119-1-lj9rcq-libre.pdf?1700434315=\u0026response-content-disposition=attachment%3B+filename%3DThe_cloning_and_sequencing_of_a_cDNA_cod.pdf\u0026Expires=1742176506\u0026Signature=KqM8o1OnfiEbMx8EKY2gZu2pZTFj~rOz9PPSv11pD2bOKnIf25DGsDv965wDMNqolTAOKlPtPpurkD7eTC30FNu7XUadiMpPRSbMaq4Ag0BsZDRAvSk833Oh76rNuVw8WO2E~4SE8CVp-AcdN~o5zroQbW0GPYj4Y7dVHlrZwND6PNRuPCmVqCprRBdOV7KPBGaXOTWqYGA4Leycp4mv8veKaJ2ZmaBhMXeK~cSQQ1xIBfzm7zcMRhbvVAt4yMtugy6tbXgIx~tZSMYdl0271SIeT~G8OKQd7lYQZrl4SS8EeTdUB8dLN5-Gwjo~PZHRLSNTpNgt5Eez0E9HuA7LZg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA"},{"id":112338,"name":"Oxidoreductases","url":"https://www.academia.edu/Documents/in/Oxidoreductases"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene"},{"id":233229,"name":"Genes","url":"https://www.academia.edu/Documents/in/Genes"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":585573,"name":"Amino Acid Profile","url":"https://www.academia.edu/Documents/in/Amino_Acid_Profile"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":899568,"name":"Tyrosinase","url":"https://www.academia.edu/Documents/in/Tyrosinase"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"}],"urls":[{"id":35559959,"url":"https://api.elsevier.com/content/article/PII:S0167478197001449?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="109411758"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/109411758/Transcriptional_and_electrophysiological_aberrations_in_an_induced_pluripotent_stem_cell_derived_model_of_spinocerebellar_ataxia_type_7"><img alt="Research paper thumbnail of Transcriptional and electrophysiological aberrations in an induced pluripotent stem cell-derived model of spinocerebellar ataxia type 7" class="work-thumbnail" src="https://attachments.academia-assets.com/107544887/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/109411758/Transcriptional_and_electrophysiological_aberrations_in_an_induced_pluripotent_stem_cell_derived_model_of_spinocerebellar_ataxia_type_7">Transcriptional and electrophysiological aberrations in an induced pluripotent stem cell-derived model of spinocerebellar ataxia type 7</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Spinocerebellar ataxia type 7 (SCA7) is an inherited neurodegenerative disease that is characteri...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Spinocerebellar ataxia type 7 (SCA7) is an inherited neurodegenerative disease that is characterised by ataxia and visual loss. It results from a degeneration of cerebellar Purkinje neurons and retinal photoreceptors caused by a polyglutamine repeat expansion in the ATXN7 gene, a component of the STAGA transcription co-activator complex. As with many neurodegenerative diseases, studies of pathogenesis have been hindered by a lack of disease-relevant models. To this end, we have generated the first induced pluripotent stem cells (iPSCs) from South African SCA7 patients, where the disease occurs at an unusually high frequency as a result of a founder effect. These iPSCs were capable of differentiation into neural and retinal cells, and showed evidence of a transcriptional phenotype affecting components of STAGA (ATXN7 and KAT2A) and the heat shock protein pathway (DNAJA1 and HSP70). Functionally, SCA7 iPSC-derived neurons exhibited more negative resting membrane potentials and increas...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6bed4b0c761d150a2d94789afc808a58" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":107544887,"asset_id":109411758,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/107544887/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="109411758"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="109411758"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 109411758; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=109411758]").text(description); $(".js-view-count[data-work-id=109411758]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 109411758; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='109411758']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6bed4b0c761d150a2d94789afc808a58" } } $('.js-work-strip[data-work-id=109411758]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":109411758,"title":"Transcriptional and electrophysiological aberrations in an induced pluripotent stem cell-derived model of spinocerebellar ataxia type 7","translated_title":"","metadata":{"abstract":"Spinocerebellar ataxia type 7 (SCA7) is an inherited neurodegenerative disease that is characterised by ataxia and visual loss. 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Although photodynamic mechanisms (i.e. through endogenous photosensitizers) play a role in UVA phototherapy for the treatment of skin disorders such as eczema and psoriasis, photodynamic therapy employing exogenous photosensitizers are currently being used only for the treatment of certain forms of non-melanoma skin cancers and actinic keratoses. There are few reports however on its use in treating melanomas. This in vitro study analyses the phototoxic effect of UVA (400-315 nm)-activated hypericin in human pigmented and unpigmented melanomas and immortalised keratinocytes and melanocytes. We show that neither hypericin exposure nor UV irradiation alone reduces cell viability. We show that an exposure to 1 lM UVA-activated hypericin does not bring about cell death, while 3 lM activated hypericin induces a necrotic mode of cell death in pigmented melanoma cells and melanocytes and an apoptotic mode of cell death in non-pigmented melanoma cells and keratinocytes. We hypothesis that the necrotic mode of cell death in the pigmented cells is possibly related to the presence of melanin-containing melanosomes in these cells and that the hypericin-induced increase in reactive oxygen species leads to an increase in permeability of melanosomes. This would result in toxic melanin precursors (of an indolic and phenolic nature) leaking into the cytoplasm which in turn leads to cell death. Hypericin localisation in the endoplasmic reticulum in these cells shown by fluorescent microscopy, further support a disruption in cellular processing and induction of cell death. In contrast, this study shows that cells that do not contain melanosomes (non-pigmented melanoma cells and keratinocytes) die by apoptosis. Further, using a mitochondrial-specific fluorescent dye, we show that intracellular accumulation of hypericin induces a mitochondrial-associated caspase-dependent apoptotic mode of cell death. This work suggests that UVA is effective in activating hypericin and that this phototoxicity may be considered as treatment option in some cases of lentigo maligna or lentigo maligna melanoma that are too large for surgical resection.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ee5c0b1a96cf13e4337dc9d305922df5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":96615731,"asset_id":94047031,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/96615731/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94047031"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94047031"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94047031; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94047031]").text(description); $(".js-view-count[data-work-id=94047031]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94047031; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94047031']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ee5c0b1a96cf13e4337dc9d305922df5" } } $('.js-work-strip[data-work-id=94047031]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94047031,"title":"Hypericin phototoxicity induces different modes of cell death in melanoma and human skin cells","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Hypericin, the major component of St. John's Wort, absorbs light in the UV and visible ranges whereupon it becomes phototoxic through the production of reactive oxygen species. Although photodynamic mechanisms (i.e. through endogenous photosensitizers) play a role in UVA phototherapy for the treatment of skin disorders such as eczema and psoriasis, photodynamic therapy employing exogenous photosensitizers are currently being used only for the treatment of certain forms of non-melanoma skin cancers and actinic keratoses. There are few reports however on its use in treating melanomas. This in vitro study analyses the phototoxic effect of UVA (400-315 nm)-activated hypericin in human pigmented and unpigmented melanomas and immortalised keratinocytes and melanocytes. We show that neither hypericin exposure nor UV irradiation alone reduces cell viability. We show that an exposure to 1 lM UVA-activated hypericin does not bring about cell death, while 3 lM activated hypericin induces a necrotic mode of cell death in pigmented melanoma cells and melanocytes and an apoptotic mode of cell death in non-pigmented melanoma cells and keratinocytes. We hypothesis that the necrotic mode of cell death in the pigmented cells is possibly related to the presence of melanin-containing melanosomes in these cells and that the hypericin-induced increase in reactive oxygen species leads to an increase in permeability of melanosomes. This would result in toxic melanin precursors (of an indolic and phenolic nature) leaking into the cytoplasm which in turn leads to cell death. Hypericin localisation in the endoplasmic reticulum in these cells shown by fluorescent microscopy, further support a disruption in cellular processing and induction of cell death. In contrast, this study shows that cells that do not contain melanosomes (non-pigmented melanoma cells and keratinocytes) die by apoptosis. Further, using a mitochondrial-specific fluorescent dye, we show that intracellular accumulation of hypericin induces a mitochondrial-associated caspase-dependent apoptotic mode of cell death. 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Although photodynamic mechanisms (i.e. through endogenous photosensitizers) play a role in UVA phototherapy for the treatment of skin disorders such as eczema and psoriasis, photodynamic therapy employing exogenous photosensitizers are currently being used only for the treatment of certain forms of non-melanoma skin cancers and actinic keratoses. There are few reports however on its use in treating melanomas. This in vitro study analyses the phototoxic effect of UVA (400-315 nm)-activated hypericin in human pigmented and unpigmented melanomas and immortalised keratinocytes and melanocytes. We show that neither hypericin exposure nor UV irradiation alone reduces cell viability. We show that an exposure to 1 lM UVA-activated hypericin does not bring about cell death, while 3 lM activated hypericin induces a necrotic mode of cell death in pigmented melanoma cells and melanocytes and an apoptotic mode of cell death in non-pigmented melanoma cells and keratinocytes. We hypothesis that the necrotic mode of cell death in the pigmented cells is possibly related to the presence of melanin-containing melanosomes in these cells and that the hypericin-induced increase in reactive oxygen species leads to an increase in permeability of melanosomes. This would result in toxic melanin precursors (of an indolic and phenolic nature) leaking into the cytoplasm which in turn leads to cell death. Hypericin localisation in the endoplasmic reticulum in these cells shown by fluorescent microscopy, further support a disruption in cellular processing and induction of cell death. In contrast, this study shows that cells that do not contain melanosomes (non-pigmented melanoma cells and keratinocytes) die by apoptosis. Further, using a mitochondrial-specific fluorescent dye, we show that intracellular accumulation of hypericin induces a mitochondrial-associated caspase-dependent apoptotic mode of cell death. This work suggests that UVA is effective in activating hypericin and that this phototoxicity may be considered as treatment option in some cases of lentigo maligna or lentigo maligna melanoma that are too large for surgical resection.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":96615731,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96615731/thumbnails/1.jpg","file_name":"j.jphotobiol.2008.01.01120221231-1-1aiv87j.pdf","download_url":"https://www.academia.edu/attachments/96615731/download_file","bulk_download_file_name":"Hypericin_phototoxicity_induces_differen.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96615731/j.jphotobiol.2008.01.01120221231-1-1aiv87j-libre.pdf?1672492207=\u0026response-content-disposition=attachment%3B+filename%3DHypericin_phototoxicity_induces_differen.pdf\u0026Expires=1742176506\u0026Signature=XSuI8b56cnr64Mua3-kudRQzO1sbyjx75pR5JQe7c0231QdsKR-KcjYImuL~9sz2oL~ab2wx1k9o1figXMv~bkgNIbQxST~8iMEdJBrBNpoTNjJHLXJibkeXPr6RwyK2CFdo0Qm50Kk6iSha9v6JSwdfZi~uHnFvzoGpwgDGYHibsrp98YVKq4bROjOZM90pXW6~j8RGzvvRzkyCD-GNRH2ENM~Az3ECT4Wwp1ZlEb0k0OFG2YGUYDtbGVtnrP5ioQUOvLsYcDSuWqFx9lJIYuQFG2ck0u5SrmtWVFEN199T6mmQSN0S~27MAQgxGYvDFiEIjxo2J1kAcj3CcbkXxA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7631,"name":"Melanin","url":"https://www.academia.edu/Documents/in/Melanin"},{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":70552,"name":"Keratinocytes","url":"https://www.academia.edu/Documents/in/Keratinocytes"},{"id":99023,"name":"Melanoma","url":"https://www.academia.edu/Documents/in/Melanoma"},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death"},{"id":122187,"name":"Endoplasmic Reticulum","url":"https://www.academia.edu/Documents/in/Endoplasmic_Reticulum"},{"id":165002,"name":"Cell Viability","url":"https://www.academia.edu/Documents/in/Cell_Viability"},{"id":247477,"name":"Caspase","url":"https://www.academia.edu/Documents/in/Caspase"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":780594,"name":"In Vitro Studies","url":"https://www.academia.edu/Documents/in/In_Vitro_Studies"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival"},{"id":1311259,"name":"Intracellular Space","url":"https://www.academia.edu/Documents/in/Intracellular_Space"},{"id":1344396,"name":"Hypericin","url":"https://www.academia.edu/Documents/in/Hypericin"},{"id":1348005,"name":"Melanosome","url":"https://www.academia.edu/Documents/in/Melanosome"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"},{"id":3826324,"name":"Human skin","url":"https://www.academia.edu/Documents/in/Human_skin-1"}],"urls":[{"id":27605768,"url":"https://api.elsevier.com/content/article/PII:S101113440800033X?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94047028"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94047028/Immunofluorescent_Identification_of_Melanocytes_in_Murine_Hair_Follicles"><img alt="Research paper thumbnail of Immunofluorescent Identification of Melanocytes in Murine Hair Follicles" class="work-thumbnail" src="https://attachments.academia-assets.com/96615709/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94047028/Immunofluorescent_Identification_of_Melanocytes_in_Murine_Hair_Follicles">Immunofluorescent Identification of Melanocytes in Murine Hair Follicles</a></div><div class="wp-workCard_item"><span>Journal of Molecular Histology</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluorescent components within the cell and the environment. This is particularly evident in hair follicles. This paper reports on a serendipitous modification to an existing method which results in a drastically reduced background fluorescence. Immediately after antigen retrieval, sections exposed to 0.3% hydrogen peroxide in methanol for 30 min at room temperature exhibited low background fluorescence, increased antigenicity and revealed quantifiable numbers of melanocytes. This method is applicable to both human and mouse melanocytes particularly in the hair follicle.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="df6379294e603c5581003b8e09f7807a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":96615709,"asset_id":94047028,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/96615709/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94047028"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94047028"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94047028; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94047028]").text(description); $(".js-view-count[data-work-id=94047028]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94047028; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94047028']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "df6379294e603c5581003b8e09f7807a" } } $('.js-work-strip[data-work-id=94047028]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94047028,"title":"Immunofluorescent Identification of Melanocytes in Murine Hair Follicles","translated_title":"","metadata":{"publisher":"Springer Science and Business Media LLC","ai_title_tag":"Reduced Background Immunofluorescence in Hair Follicles","grobid_abstract":"Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluorescent components within the cell and the environment. 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This method is applicable to both human and mouse melanocytes particularly in the hair follicle.","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Journal of Molecular Histology","grobid_abstract_attachment_id":96615709},"translated_abstract":null,"internal_url":"https://www.academia.edu/94047028/Immunofluorescent_Identification_of_Melanocytes_in_Murine_Hair_Follicles","translated_internal_url":"","created_at":"2022-12-31T05:07:06.254-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":96615709,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96615709/thumbnails/1.jpg","file_name":"s10735-005-9011-820221231-1-1c3byhy.pdf","download_url":"https://www.academia.edu/attachments/96615709/download_file","bulk_download_file_name":"Immunofluorescent_Identification_of_Mela.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96615709/s10735-005-9011-820221231-1-1c3byhy-libre.pdf?1672492204=\u0026response-content-disposition=attachment%3B+filename%3DImmunofluorescent_Identification_of_Mela.pdf\u0026Expires=1742176506\u0026Signature=SwER-YyyUhRmOOPVdxg2IAGdmsYvZLEfOEYmpm4di~mBXBOS7Jrd8~8S0hkXiEivruCUqTQ2~dd7W1egz6fBWvybMBIIfV~kaegX-Od~c7GeNNCNXrTCY-kJ8LqiRKyKRHX-3MJ0qQ7zl34reManhAtUfACMYsfOugHCWe-oT8XuIYpW0nYpZ0ovOKmfZUtauSRKYJidD1i8K8BGOUfvBN222x0moXhf4r-R~GJ6vKCYAbBInGS-gqKo1SX3nSLb-rsy25p9bA7SfEMqyhMzcTcv2FQB4Gc1ktTS-WuzjHSRRQ9FtzUCcGeZQYMU-PW1y6aGpQ8TwGdKrILdhEhXXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Immunofluorescent_Identification_of_Melanocytes_in_Murine_Hair_Follicles","translated_slug":"","page_count":3,"language":"en","content_type":"Work","summary":"Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluorescent components within the cell and the environment. This is particularly evident in hair follicles. This paper reports on a serendipitous modification to an existing method which results in a drastically reduced background fluorescence. Immediately after antigen retrieval, sections exposed to 0.3% hydrogen peroxide in methanol for 30 min at room temperature exhibited low background fluorescence, increased antigenicity and revealed quantifiable numbers of melanocytes. This method is applicable to both human and mouse melanocytes particularly in the hair follicle.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":96615709,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/96615709/thumbnails/1.jpg","file_name":"s10735-005-9011-820221231-1-1c3byhy.pdf","download_url":"https://www.academia.edu/attachments/96615709/download_file","bulk_download_file_name":"Immunofluorescent_Identification_of_Mela.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/96615709/s10735-005-9011-820221231-1-1c3byhy-libre.pdf?1672492204=\u0026response-content-disposition=attachment%3B+filename%3DImmunofluorescent_Identification_of_Mela.pdf\u0026Expires=1742176506\u0026Signature=SwER-YyyUhRmOOPVdxg2IAGdmsYvZLEfOEYmpm4di~mBXBOS7Jrd8~8S0hkXiEivruCUqTQ2~dd7W1egz6fBWvybMBIIfV~kaegX-Od~c7GeNNCNXrTCY-kJ8LqiRKyKRHX-3MJ0qQ7zl34reManhAtUfACMYsfOugHCWe-oT8XuIYpW0nYpZ0ovOKmfZUtauSRKYJidD1i8K8BGOUfvBN222x0moXhf4r-R~GJ6vKCYAbBInGS-gqKo1SX3nSLb-rsy25p9bA7SfEMqyhMzcTcv2FQB4Gc1ktTS-WuzjHSRRQ9FtzUCcGeZQYMU-PW1y6aGpQ8TwGdKrILdhEhXXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide"},{"id":391252,"name":"Room Temperature","url":"https://www.academia.edu/Documents/in/Room_Temperature"},{"id":568482,"name":"Biological markers","url":"https://www.academia.edu/Documents/in/Biological_markers"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":1123231,"name":"Hair Follicle","url":"https://www.academia.edu/Documents/in/Hair_Follicle"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"},{"id":2007902,"name":"Antigenicity","url":"https://www.academia.edu/Documents/in/Antigenicity"},{"id":3789879,"name":"Cardiovascular medicine and haematology","url":"https://www.academia.edu/Documents/in/Cardiovascular_medicine_and_haematology"}],"urls":[{"id":27605765,"url":"http://link.springer.com/content/pdf/10.1007/s10735-005-9011-8.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="94046999"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/94046999/The_effect_of_temperature_on_tyrosinase_activity_in_Himalayan_mouse_skin"><img alt="Research paper thumbnail of The effect of temperature on tyrosinase activity in Himalayan mouse skin" class="work-thumbnail" src="https://attachments.academia-assets.com/96615673/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/94046999/The_effect_of_temperature_on_tyrosinase_activity_in_Himalayan_mouse_skin">The effect of temperature on tyrosinase activity in Himalayan mouse skin</a></div><div class="wp-workCard_item"><span>The Journal of experimental zoology</span><span>, 1981</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The tyrosinase activity of Himalayan mouse skin homogenates was measured over a range of temperat...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The tyrosinase activity of Himalayan mouse skin homogenates was measured over a range of temperatures using two sensitive radiometric assay--namely, (1) the measurement of 14C-tyrosine incorporation into melanin, and (2) the measurement of 3HOH released as a by-product of 3H-tyrosine hydroxylation. Results show that Himalayan tyrosinase is maximally active at temperatures well below normal body temperature (15 degree C to 25 degree C). These results are in support of Danneel&#39;s visual observations (&#39;41) that &quot;ferment&quot; activity of Himalayan rabbit skin is absent at temperatures above 25 degree C. Further results suggest the presence of a tyrosinase inhibitor in Himalayan mouse skin. First, removal of a low molecular weight fraction from Himalayan skin homogenates resulted in an increase in tyrosinase activity. Second, recombination of the low molecular weight fraction to the homogenate from which it was originally separated resulted in a decrease in tyrosinase activi...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0e81a4a3865353bdf8ad06eaf9a45959" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":96615673,"asset_id":94046999,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/96615673/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="94046999"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="94046999"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 94046999; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=94046999]").text(description); $(".js-view-count[data-work-id=94046999]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 94046999; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='94046999']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0e81a4a3865353bdf8ad06eaf9a45959" } } $('.js-work-strip[data-work-id=94046999]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":94046999,"title":"The effect of temperature on tyrosinase activity in Himalayan mouse skin","translated_title":"","metadata":{"abstract":"The tyrosinase activity of Himalayan mouse skin homogenates was measured over a range of temperatures using two sensitive radiometric assay--namely, (1) the measurement of 14C-tyrosine incorporation into melanin, and (2) the measurement of 3HOH released as a by-product of 3H-tyrosine hydroxylation. Results show that Himalayan tyrosinase is maximally active at temperatures well below normal body temperature (15 degree C to 25 degree C). These results are in support of Danneel\u0026#39;s visual observations (\u0026#39;41) that \u0026quot;ferment\u0026quot; activity of Himalayan rabbit skin is absent at temperatures above 25 degree C. Further results suggest the presence of a tyrosinase inhibitor in Himalayan mouse skin. First, removal of a low molecular weight fraction from Himalayan skin homogenates resulted in an increase in tyrosinase activity. 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These results are in support of Danneel\u0026#39;s visual observations (\u0026#39;41) that \u0026quot;ferment\u0026quot; activity of Himalayan rabbit skin is absent at temperatures above 25 degree C. Further results suggest the presence of a tyrosinase inhibitor in Himalayan mouse skin. First, removal of a low molecular weight fraction from Himalayan skin homogenates resulted in an increase in tyrosinase activity. 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Although accepted as dogma, t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Vitiligo is a depigmenting disease of uncertain aetio-pathogenesis. Although accepted as dogma, the question of whether melanocytes survive in vitiligo lesions has not been adequately resolved. Defining with greater accuracy the melanocyte status of lesions would contribute greatly towards the understanding of the etiology, progression and treatment of this disorder. We have therefore revisited this issue by carrying out a molecular screen for melanocytes in lesional skin using the sensitive and specific technique of reverse transcription PCR (RT-PCR) followed by Southern blotting. Biopsies from vitiligo lesions and normal skin were obtained from 15 patients. The RT-PCR was carried out using primers for tyrosinase and dopa-chrome tautomerase (DCT). To increase the sensitivity of detection, Southern-blot analysis of all PCR products was conducted. Southern-blot analysis indicated that three lesional samples were positive: one for tyrosinase, one for DCT, and one for both. Lesions yielding positive results had been present for between 2-5 years and were inactive, as defined by no disease progression within the last 3 months. Some vitiligo lesions showed evidence of melanocyte survival, even after some years. These results open the way for the possibility of using a range of melanocyte-specific markers for molecular staging of lesional status by quantitative RT-PCR. Such information would be extremely valuable for the appropriate selection and potential success of medical therapies.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546066"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546066"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546066; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546066]").text(description); $(".js-view-count[data-work-id=83546066]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546066; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546066']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=83546066]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546066,"title":"Molecular analysis of vitiligo lesions reveals sporadic melanocyte survival","translated_title":"","metadata":{"abstract":"Vitiligo is a depigmenting disease of uncertain aetio-pathogenesis. 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Some vitiligo lesions showed evidence of melanocyte survival, even after some years. These results open the way for the possibility of using a range of melanocyte-specific markers for molecular staging of lesional status by quantitative RT-PCR. Such information would be extremely valuable for the appropriate selection and potential success of medical therapies.","internal_url":"https://www.academia.edu/83546066/Molecular_analysis_of_vitiligo_lesions_reveals_sporadic_melanocyte_survival","translated_internal_url":"","created_at":"2022-07-22T02:43:47.220-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Molecular_analysis_of_vitiligo_lesions_reveals_sporadic_melanocyte_survival","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Vitiligo is a depigmenting disease of uncertain aetio-pathogenesis. Although accepted as dogma, the question of whether melanocytes survive in vitiligo lesions has not been adequately resolved. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546064"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/83546064/The_three_dimensional_organisation_of_the_post_trabecular_aqueous_outflow_pathway_and_limbal_vasculature_in_the_mouse"><img alt="Research paper thumbnail of The three-dimensional organisation of the post-trabecular aqueous outflow pathway and limbal vasculature in the mouse" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/83546064/The_three_dimensional_organisation_of_the_post_trabecular_aqueous_outflow_pathway_and_limbal_vasculature_in_the_mouse">The three-dimensional organisation of the post-trabecular aqueous outflow pathway and limbal vasculature in the mouse</a></div><div class="wp-workCard_item"><span>Experimental eye research</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The mouse eye has been used as a model for studies on the microanatomy of the outflow pathways bu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The mouse eye has been used as a model for studies on the microanatomy of the outflow pathways but most of what is known comes from histological sections. These studies have focused mainly on the morphological features of the trabecular meshwork, Schlemm&#39;s canal and aqueous channels that link to the superficial episcleral vasculature. However, the anatomical architecture of the aqueous outflow vessels and their relationship to each other and to the general vascular circulation is not well understood. The aim of this study was to provide a detailed description of the microarchitecture of the aqueous outflow vessels and their relationship to the superficial limbal/episcleral vasculature throughout the entire limbus. The aqueous outflow vessels and blood and lymphatic vessels were imaged in PECAM-1 and LYVE-1 immunostained whole anterior segments of adult mice and three-dimensional (3-D) reconstructions of the optical sections were generated to reveal the aqueous, blood and lymphat...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546064"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546064"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546064; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546064]").text(description); $(".js-view-count[data-work-id=83546064]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546064; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546064']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=83546064]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546064,"title":"The three-dimensional organisation of the post-trabecular aqueous outflow pathway and limbal vasculature in the mouse","translated_title":"","metadata":{"abstract":"The mouse eye has been used as a model for studies on the microanatomy of the outflow pathways but most of what is known comes from histological sections. 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The aim of this study was to provide a detailed description of the microarchitecture of the aqueous outflow vessels and their relationship to the superficial limbal/episcleral vasculature throughout the entire limbus. 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Since pigment synthesis only occurs in actively growing hair, adult mice were plucked to induce hair growth. The extent of darkening of the hair was recorded by photography against a reference scale. The presence of pigment granules in hair follicles was investigated histologically. Housing adult and juvenile mice at 15 degrees C results in the synthesis of pigment in growing hair follicles whereas housing at 30 degrees C results in the absence of pigment granules in the growing hair follicles.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b942c9a047e8ec04851c8a7c00d0c7ba" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854364,"asset_id":83546062,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854364/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546062"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546062"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546062; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546062]").text(description); $(".js-view-count[data-work-id=83546062]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546062; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546062']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b942c9a047e8ec04851c8a7c00d0c7ba" } } $('.js-work-strip[data-work-id=83546062]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546062,"title":"Pigment synthesis in the Himalayan mouse","translated_title":"","metadata":{"abstract":"The effect of temperature on pigment synthesis in adult and juvenile Himalayan mice was investigated. 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Housing adult and juvenile mice at 15 degrees C results in the synthesis of pigment in growing hair follicles whereas housing at 30 degrees C results in the absence of pigment granules in the growing hair follicles.","internal_url":"https://www.academia.edu/83546062/Pigment_synthesis_in_the_Himalayan_mouse","translated_internal_url":"","created_at":"2022-07-22T02:43:46.893-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854364,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854364/thumbnails/1.jpg","file_name":"jez.140210011620220722-1-l69ny2.pdf","download_url":"https://www.academia.edu/attachments/88854364/download_file","bulk_download_file_name":"Pigment_synthesis_in_the_Himalayan_mouse.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854364/jez.140210011620220722-1-l69ny2-libre.pdf?1658483330=\u0026response-content-disposition=attachment%3B+filename%3DPigment_synthesis_in_the_Himalayan_mouse.pdf\u0026Expires=1742176506\u0026Signature=HwNZ0xSPu9bRU7~GyeizAu~CzqefzTWZ1YcSz8Qr4tb1pzltPaINJq1AWRRL5LVdLKTrAqzHWPTb1h29gk9Y3JxQmbwegju8bDYO9qLgwS-1c0029LtQzaaSD3NlRhS-yvxXiFxMpXFl2tWxnFbYsDnHwSl1wZGoBP2gWOi~kGh-VJys1W5vSJne1MAyUxQFTWdBQn3C1AJCXfhaXtL3-c1mDiqmNHkLAxNvirYEtjLXIh8CKx9qxyTN1Yzrb0BT7MqvzCdQJxAllgAfU-hqgvWAAtbAWz~VUv~cyXgPErvsN4HcgzKvc3CNJDjSgrL6rNf7EOm8eM0NrQx5xVY12A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Pigment_synthesis_in_the_Himalayan_mouse","translated_slug":"","page_count":8,"language":"en","content_type":"Work","summary":"The effect of temperature on pigment synthesis in adult and juvenile Himalayan mice was investigated. Since pigment synthesis only occurs in actively growing hair, adult mice were plucked to induce hair growth. The extent of darkening of the hair was recorded by photography against a reference scale. The presence of pigment granules in hair follicles was investigated histologically. Housing adult and juvenile mice at 15 degrees C results in the synthesis of pigment in growing hair follicles whereas housing at 30 degrees C results in the absence of pigment granules in the growing hair follicles.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854364,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854364/thumbnails/1.jpg","file_name":"jez.140210011620220722-1-l69ny2.pdf","download_url":"https://www.academia.edu/attachments/88854364/download_file","bulk_download_file_name":"Pigment_synthesis_in_the_Himalayan_mouse.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854364/jez.140210011620220722-1-l69ny2-libre.pdf?1658483330=\u0026response-content-disposition=attachment%3B+filename%3DPigment_synthesis_in_the_Himalayan_mouse.pdf\u0026Expires=1742176506\u0026Signature=HwNZ0xSPu9bRU7~GyeizAu~CzqefzTWZ1YcSz8Qr4tb1pzltPaINJq1AWRRL5LVdLKTrAqzHWPTb1h29gk9Y3JxQmbwegju8bDYO9qLgwS-1c0029LtQzaaSD3NlRhS-yvxXiFxMpXFl2tWxnFbYsDnHwSl1wZGoBP2gWOi~kGh-VJys1W5vSJne1MAyUxQFTWdBQn3C1AJCXfhaXtL3-c1mDiqmNHkLAxNvirYEtjLXIh8CKx9qxyTN1Yzrb0BT7MqvzCdQJxAllgAfU-hqgvWAAtbAWz~VUv~cyXgPErvsN4HcgzKvc3CNJDjSgrL6rNf7EOm8eM0NrQx5xVY12A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry"},{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology"},{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":3770,"name":"Metabolism","url":"https://www.academia.edu/Documents/in/Metabolism"},{"id":7666,"name":"Life history","url":"https://www.academia.edu/Documents/in/Life_history"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":10866,"name":"Morphology","url":"https://www.academia.edu/Documents/in/Morphology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":83996,"name":"Hair","url":"https://www.academia.edu/Documents/in/Hair"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature"},{"id":162553,"name":"Skin","url":"https://www.academia.edu/Documents/in/Skin"},{"id":233229,"name":"Genes","url":"https://www.academia.edu/Documents/in/Genes"},{"id":260028,"name":"Unknown","url":"https://www.academia.edu/Documents/in/Unknown"},{"id":493957,"name":"Organs","url":"https://www.academia.edu/Documents/in/Organs"},{"id":576640,"name":"Hair Color","url":"https://www.academia.edu/Documents/in/Hair_Color"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":976256,"name":"Skin Temperature","url":"https://www.academia.edu/Documents/in/Skin_Temperature"},{"id":1312021,"name":"Environmental Exposure","url":"https://www.academia.edu/Documents/in/Environmental_Exposure"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546061"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546061/A_congenital_defect_of_the_distal_forelimb_of_a_cat_and_of_a_dog"><img alt="Research paper thumbnail of A congenital defect of the distal forelimb of a cat and of a dog" class="work-thumbnail" src="https://attachments.academia-assets.com/88854355/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546061/A_congenital_defect_of_the_distal_forelimb_of_a_cat_and_of_a_dog">A congenital defect of the distal forelimb of a cat and of a dog</a></div><div class="wp-workCard_item"><span>Journal of the South African Veterinary Association</span><span>, 2002</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f84f577ebe3e35e311dcebd06931e9e4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854355,"asset_id":83546061,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854355/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546061"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546061"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546061; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546061]").text(description); $(".js-view-count[data-work-id=83546061]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546061; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546061']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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The cases illustrate that the defects are likely the result of in utero damage leading to limb bifurcation rather than complete duplication, with molecular interactions influencing limb growth. The report provides radiographic evidence and discusses the implications on overall limb function and development.","publication_date":{"day":null,"month":null,"year":2002,"errors":{}},"publication_name":"Journal of the South African Veterinary Association"},"translated_abstract":null,"internal_url":"https://www.academia.edu/83546061/A_congenital_defect_of_the_distal_forelimb_of_a_cat_and_of_a_dog","translated_internal_url":"","created_at":"2022-07-22T02:43:46.760-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854355,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854355/thumbnails/1.jpg","file_name":"1d105c5ae20b617ddcd90bf06ca3a8d93bb3.pdf","download_url":"https://www.academia.edu/attachments/88854355/download_file","bulk_download_file_name":"A_congenital_defect_of_the_distal_foreli.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854355/1d105c5ae20b617ddcd90bf06ca3a8d93bb3-libre.pdf?1658483327=\u0026response-content-disposition=attachment%3B+filename%3DA_congenital_defect_of_the_distal_foreli.pdf\u0026Expires=1742176506\u0026Signature=IfHBCO0ara1401QvwrmFJXe3OHdkSjz7JKv0CfLFTnSaY4AX0NPhNB48V-WDvGkxR0uJbO3W0OXeJVZA4mklZBerP9xOHs-kIf0f4~iW2E8EOUrmKIbsjgDpL00wl5VX30WF4EPVjyumBQyUloOEuavWpX98kzXUJQtcIEA-dvz-z7MM-dhoZC0FOKc9PIzAi2hpq1~EfIa1h5xVN4B7jUK3I0tLRxzU34IDVBdboW1St266vsUCU~Jeas~coJcVoVUGaiiUOxowYESSXK1WTOa5J0iqJlwHQYWfSvlBKwC6xPailn5uYzqla1Tqr8OhSzVmqrM4Kn2bwjBWHdFwXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"A_congenital_defect_of_the_distal_forelimb_of_a_cat_and_of_a_dog","translated_slug":"","page_count":2,"language":"en","content_type":"Work","summary":null,"owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854355,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854355/thumbnails/1.jpg","file_name":"1d105c5ae20b617ddcd90bf06ca3a8d93bb3.pdf","download_url":"https://www.academia.edu/attachments/88854355/download_file","bulk_download_file_name":"A_congenital_defect_of_the_distal_foreli.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854355/1d105c5ae20b617ddcd90bf06ca3a8d93bb3-libre.pdf?1658483327=\u0026response-content-disposition=attachment%3B+filename%3DA_congenital_defect_of_the_distal_foreli.pdf\u0026Expires=1742176506\u0026Signature=IfHBCO0ara1401QvwrmFJXe3OHdkSjz7JKv0CfLFTnSaY4AX0NPhNB48V-WDvGkxR0uJbO3W0OXeJVZA4mklZBerP9xOHs-kIf0f4~iW2E8EOUrmKIbsjgDpL00wl5VX30WF4EPVjyumBQyUloOEuavWpX98kzXUJQtcIEA-dvz-z7MM-dhoZC0FOKc9PIzAi2hpq1~EfIa1h5xVN4B7jUK3I0tLRxzU34IDVBdboW1St266vsUCU~Jeas~coJcVoVUGaiiUOxowYESSXK1WTOa5J0iqJlwHQYWfSvlBKwC6xPailn5uYzqla1Tqr8OhSzVmqrM4Kn2bwjBWHdFwXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":41088,"name":"Cats","url":"https://www.academia.edu/Documents/in/Cats"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs"},{"id":54589,"name":"Anatomy","url":"https://www.academia.edu/Documents/in/Anatomy"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":4055611,"name":"Fatal outcome","url":"https://www.academia.edu/Documents/in/Fatal_outcome"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546060"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546060/A_New_Class_of_Stem_Cells_in_South_Africa_Introducing_Induced_Pluripotent_Stem_cells_iPS_cells_"><img alt="Research paper thumbnail of A New Class of Stem Cells in South Africa: Introducing Induced Pluripotent Stem cells (iPS cells)" class="work-thumbnail" src="https://attachments.academia-assets.com/88854356/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546060/A_New_Class_of_Stem_Cells_in_South_Africa_Introducing_Induced_Pluripotent_Stem_cells_iPS_cells_">A New Class of Stem Cells in South Africa: Introducing Induced Pluripotent Stem cells (iPS cells)</a></div><div class="wp-workCard_item"><span>South African Medical Journal</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="040bb7eeeefb485c5914e38d374b9f8b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854356,"asset_id":83546060,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854356/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546060"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546060"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546060; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546059"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546059/Closely_linked_early_and_late_histone_H2B_genes_are_differentially_expressed_after_microinjection_into_sea_urchin_zygotes"><img alt="Research paper thumbnail of Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes" class="work-thumbnail" src="https://attachments.academia-assets.com/88854358/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546059/Closely_linked_early_and_late_histone_H2B_genes_are_differentially_expressed_after_microinjection_into_sea_urchin_zygotes">Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes</a></div><div class="wp-workCard_item"><span>Proceedings of the National Academy of Sciences</span><span>, 1988</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linke...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linked in a single plasmid and injected into the eggs of the sea urchin Lytechinus pictus. The levels of transcripts of injected early and late genes and of endogenous early genes were monitored during development by a ribonuclease protection assay. Transcripts of both the injected and endogenous early genes peaked during the blastula stage and decreased severalfold by the mesenchyme blastula stage. Transcripts of the injected late gene became detectable at the blastula stage and increased in amount subsequently, until at least the early gastrula stage, 28 hr after fertilization. Thus, the pattern of expression of the injected early and late H2B genes is similar to that of their endogenous counterparts. These results show that DNA sequences regulating the temporal pattern of early and late H2B gene expression must lie within the cloned DNA segments; i.e., within 600 base pairs of the early H2...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4227c1bec1e0a648e7e39efac3812de6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854358,"asset_id":83546059,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854358/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546059"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546059"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546059; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546059]").text(description); $(".js-view-count[data-work-id=83546059]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546059; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546059']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4227c1bec1e0a648e7e39efac3812de6" } } $('.js-work-strip[data-work-id=83546059]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546059,"title":"Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes","translated_title":"","metadata":{"abstract":"An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linked in a single plasmid and injected into the eggs of the sea urchin Lytechinus pictus. 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These results show that DNA sequences regulating the temporal pattern of early and late H2B gene expression must lie within the cloned DNA segments; i.e., within 600 base pairs of the early H2...","publisher":"Proceedings of the National Academy of Sciences","publication_date":{"day":null,"month":null,"year":1988,"errors":{}},"publication_name":"Proceedings of the National Academy of Sciences"},"translated_abstract":"An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linked in a single plasmid and injected into the eggs of the sea urchin Lytechinus pictus. The levels of transcripts of injected early and late genes and of endogenous early genes were monitored during development by a ribonuclease protection assay. Transcripts of both the injected and endogenous early genes peaked during the blastula stage and decreased severalfold by the mesenchyme blastula stage. Transcripts of the injected late gene became detectable at the blastula stage and increased in amount subsequently, until at least the early gastrula stage, 28 hr after fertilization. Thus, the pattern of expression of the injected early and late H2B genes is similar to that of their endogenous counterparts. These results show that DNA sequences regulating the temporal pattern of early and late H2B gene expression must lie within the cloned DNA segments; i.e., within 600 base pairs of the early H2...","internal_url":"https://www.academia.edu/83546059/Closely_linked_early_and_late_histone_H2B_genes_are_differentially_expressed_after_microinjection_into_sea_urchin_zygotes","translated_internal_url":"","created_at":"2022-07-22T02:43:46.486-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854358,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854358/thumbnails/1.jpg","file_name":"507.full.pdf","download_url":"https://www.academia.edu/attachments/88854358/download_file","bulk_download_file_name":"Closely_linked_early_and_late_histone_H2.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854358/507.full-libre.pdf?1658483329=\u0026response-content-disposition=attachment%3B+filename%3DClosely_linked_early_and_late_histone_H2.pdf\u0026Expires=1742176507\u0026Signature=CfUCTWk7TrqCFmY3-81AWqiWC3NaK1vxfmUoGlOGvcI4bVjWOCsBDp-3b4R-O6pA93YTMW9JcY5hIW2STNXtCrxyKN3luUiReeRJPsy3iSk9klL7-~RfXSYrjFnymgvJhLOks9lxryMwGzXlMWHNHi7V8aKphynfhXqIp0FTAyhyv60tTyh4YXEQibOcIX4orvxFP6MEXYnkPK3z0CJsFU1maa67wK14IIe67K2-YQ7EhmNcH0EJhpg94ZbqLaSycjpj7qDHsHkykBx4hKs2gIVz8s0JFuA2k-Q6liDYIUe9TvtqpHqEvkrRY4L6utlPCOVJM3slJ8u0-8RW~DWbwA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Closely_linked_early_and_late_histone_H2B_genes_are_differentially_expressed_after_microinjection_into_sea_urchin_zygotes","translated_slug":"","page_count":4,"language":"en","content_type":"Work","summary":"An early and a late histone H2B gene from the sea urchin Stronglyocentrotus purpuratus were linked in a single plasmid and injected into the eggs of the sea urchin Lytechinus pictus. 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These results show that DNA sequences regulating the temporal pattern of early and late H2B gene expression must lie within the cloned DNA segments; i.e., within 600 base pairs of the early H2...","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854358,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854358/thumbnails/1.jpg","file_name":"507.full.pdf","download_url":"https://www.academia.edu/attachments/88854358/download_file","bulk_download_file_name":"Closely_linked_early_and_late_histone_H2.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854358/507.full-libre.pdf?1658483329=\u0026response-content-disposition=attachment%3B+filename%3DClosely_linked_early_and_late_histone_H2.pdf\u0026Expires=1742176507\u0026Signature=CfUCTWk7TrqCFmY3-81AWqiWC3NaK1vxfmUoGlOGvcI4bVjWOCsBDp-3b4R-O6pA93YTMW9JcY5hIW2STNXtCrxyKN3luUiReeRJPsy3iSk9klL7-~RfXSYrjFnymgvJhLOks9lxryMwGzXlMWHNHi7V8aKphynfhXqIp0FTAyhyv60tTyh4YXEQibOcIX4orvxFP6MEXYnkPK3z0CJsFU1maa67wK14IIe67K2-YQ7EhmNcH0EJhpg94ZbqLaSycjpj7qDHsHkykBx4hKs2gIVz8s0JFuA2k-Q6liDYIUe9TvtqpHqEvkrRY4L6utlPCOVJM3slJ8u0-8RW~DWbwA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":11035,"name":"Regulation","url":"https://www.academia.edu/Documents/in/Regulation"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":190363,"name":"Plasmids","url":"https://www.academia.edu/Documents/in/Plasmids"},{"id":229847,"name":"Sea Urchins","url":"https://www.academia.edu/Documents/in/Sea_Urchins"},{"id":233229,"name":"Genes","url":"https://www.academia.edu/Documents/in/Genes"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning"},{"id":317185,"name":"Histones","url":"https://www.academia.edu/Documents/in/Histones"},{"id":560354,"name":"Sea Urchin","url":"https://www.academia.edu/Documents/in/Sea_Urchin"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes"},{"id":2274872,"name":"DNA sequence","url":"https://www.academia.edu/Documents/in/DNA_sequence"},{"id":2280042,"name":"Differential expression","url":"https://www.academia.edu/Documents/in/Differential_expression"},{"id":3647178,"name":"DNA Restriction Enzymes","url":"https://www.academia.edu/Documents/in/DNA_Restriction_Enzymes"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546058"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/83546058/The_Regulation_of_Tyrosinase_Gene_Transcription"><img alt="Research paper thumbnail of The Regulation of Tyrosinase Gene Transcription" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/83546058/The_Regulation_of_Tyrosinase_Gene_Transcription">The Regulation of Tyrosinase Gene Transcription</a></div><div class="wp-workCard_item"><span>Pigment Cell Research</span><span>, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity res...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity rests in part at the level of transcriptional regulation. The 5&amp;#39; promoter regions of the human, mouse, chicken, quail, snapping turtle, and frog tyrosinase sequences have been isolated and the mechanisms regulating the activity of these sequences are beginning to be elucidated. This review provides an update on the following aspects of tyrosinase gene regulation: basal promoter elements that determine the site of transcription initiation for RNA polymerase II; the cis-acting elements and DNA-binding factors that mediate melanocyte-specific expression of the tyrosinase gene; promoter elements involved in the temporal control of tyrosinase gene expression; additional elements that may be required to achieve wild-type levels of gene expression; and specific elements that may be required for modulation of tyrosinase gene expression in response to humoral factors or external stimuli that are known to influence the amounts of melanin synthesized by fully differentiated melanocytes. The wild type expression of tyrosinase is the result of the interaction of many different factors and it is becoming evident that certain elements and factors play more than one role in this process.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546058"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546058"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546058; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546058]").text(description); $(".js-view-count[data-work-id=83546058]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546058; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546058']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=83546058]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546058,"title":"The Regulation of Tyrosinase Gene Transcription","translated_title":"","metadata":{"abstract":"Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity rests in part at the level of transcriptional regulation. The 5\u0026amp;#39; promoter regions of the human, mouse, chicken, quail, snapping turtle, and frog tyrosinase sequences have been isolated and the mechanisms regulating the activity of these sequences are beginning to be elucidated. This review provides an update on the following aspects of tyrosinase gene regulation: basal promoter elements that determine the site of transcription initiation for RNA polymerase II; the cis-acting elements and DNA-binding factors that mediate melanocyte-specific expression of the tyrosinase gene; promoter elements involved in the temporal control of tyrosinase gene expression; additional elements that may be required to achieve wild-type levels of gene expression; and specific elements that may be required for modulation of tyrosinase gene expression in response to humoral factors or external stimuli that are known to influence the amounts of melanin synthesized by fully differentiated melanocytes. The wild type expression of tyrosinase is the result of the interaction of many different factors and it is becoming evident that certain elements and factors play more than one role in this process.","publisher":"Wiley","publication_date":{"day":null,"month":null,"year":1997,"errors":{}},"publication_name":"Pigment Cell Research"},"translated_abstract":"Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity rests in part at the level of transcriptional regulation. The 5\u0026amp;#39; promoter regions of the human, mouse, chicken, quail, snapping turtle, and frog tyrosinase sequences have been isolated and the mechanisms regulating the activity of these sequences are beginning to be elucidated. This review provides an update on the following aspects of tyrosinase gene regulation: basal promoter elements that determine the site of transcription initiation for RNA polymerase II; the cis-acting elements and DNA-binding factors that mediate melanocyte-specific expression of the tyrosinase gene; promoter elements involved in the temporal control of tyrosinase gene expression; additional elements that may be required to achieve wild-type levels of gene expression; and specific elements that may be required for modulation of tyrosinase gene expression in response to humoral factors or external stimuli that are known to influence the amounts of melanin synthesized by fully differentiated melanocytes. The wild type expression of tyrosinase is the result of the interaction of many different factors and it is becoming evident that certain elements and factors play more than one role in this process.","internal_url":"https://www.academia.edu/83546058/The_Regulation_of_Tyrosinase_Gene_Transcription","translated_internal_url":"","created_at":"2022-07-22T02:43:46.356-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_Regulation_of_Tyrosinase_Gene_Transcription","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Tyrosinase is one of the key enzymes essential for melanogenesis. The control of its activity rests in part at the level of transcriptional regulation. The 5\u0026amp;#39; promoter regions of the human, mouse, chicken, quail, snapping turtle, and frog tyrosinase sequences have been isolated and the mechanisms regulating the activity of these sequences are beginning to be elucidated. This review provides an update on the following aspects of tyrosinase gene regulation: basal promoter elements that determine the site of transcription initiation for RNA polymerase II; the cis-acting elements and DNA-binding factors that mediate melanocyte-specific expression of the tyrosinase gene; promoter elements involved in the temporal control of tyrosinase gene expression; additional elements that may be required to achieve wild-type levels of gene expression; and specific elements that may be required for modulation of tyrosinase gene expression in response to humoral factors or external stimuli that are known to influence the amounts of melanin synthesized by fully differentiated melanocytes. The wild type expression of tyrosinase is the result of the interaction of many different factors and it is becoming evident that certain elements and factors play more than one role in this process.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":23323,"name":"Transcription Factors","url":"https://www.academia.edu/Documents/in/Transcription_Factors"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":92680,"name":"cyclic AMP","url":"https://www.academia.edu/Documents/in/cyclic_AMP"},{"id":103254,"name":"Vertebrates","url":"https://www.academia.edu/Documents/in/Vertebrates"},{"id":162553,"name":"Skin","url":"https://www.academia.edu/Documents/in/Skin"},{"id":349211,"name":"Skin Pigmentation","url":"https://www.academia.edu/Documents/in/Skin_Pigmentation"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":591594,"name":"Tretinoin","url":"https://www.academia.edu/Documents/in/Tretinoin"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":924535,"name":"Quail","url":"https://www.academia.edu/Documents/in/Quail"},{"id":1293308,"name":"Glucocorticoids","url":"https://www.academia.edu/Documents/in/Glucocorticoids"},{"id":1405352,"name":"Pigment Cell","url":"https://www.academia.edu/Documents/in/Pigment_Cell"},{"id":2153835,"name":"Enzyme Induction","url":"https://www.academia.edu/Documents/in/Enzyme_Induction"},{"id":3005391,"name":"Ultraviolet Rays","url":"https://www.academia.edu/Documents/in/Ultraviolet_Rays"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":22355844,"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0749.1997.tb00474.x"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546057"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546057/Red_or_rufous_albinism_in_Southern_Africa"><img alt="Research paper thumbnail of Red or rufous albinism in Southern Africa" class="work-thumbnail" src="https://attachments.academia-assets.com/88854354/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546057/Red_or_rufous_albinism_in_Southern_Africa">Red or rufous albinism in Southern Africa</a></div><div class="wp-workCard_item"><span>Ophthalmic Paediatrics and Genetics</span><span>, 1990</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">As the Alzheimer's disease process progresses in time measurements of cognitive functioning exhib...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">As the Alzheimer's disease process progresses in time measurements of cognitive functioning exhibit nonlinearity. Multiphase models were used to quantify this nonlinearity for thirty-six well characterized individuals(∼12 observations per individual over ∼15 years in the study) by partitioning each into a healthy aging phase and a diseased phase. This enabled us to detail both the magnitude and timing that Alzheimer's disease alters different aspects of cognitive function. Estimation of these models was done using Bayesian methods. Eight different outcomes representing three areas of memory functioning(visual, verbal, working) were used to define a pattern of cognitive decline. The earliest phase change was found to be visual memory(∼6 years before diagnosis) and was followed by changes in verbal and working memory beginning roughly four years later.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cc7e9d9043c929c6a5d101012aa11519" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854354,"asset_id":83546057,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854354/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546057"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546057"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546057; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546057]").text(description); $(".js-view-count[data-work-id=83546057]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546057; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546057']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cc7e9d9043c929c6a5d101012aa11519" } } $('.js-work-strip[data-work-id=83546057]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546057,"title":"Red or rufous albinism in Southern Africa","translated_title":"","metadata":{"publisher":"Informa UK Limited","grobid_abstract":"As the Alzheimer's disease process progresses in time measurements of cognitive functioning exhibit nonlinearity. Multiphase models were used to quantify this nonlinearity for thirty-six well characterized individuals(∼12 observations per individual over ∼15 years in the study) by partitioning each into a healthy aging phase and a diseased phase. This enabled us to detail both the magnitude and timing that Alzheimer's disease alters different aspects of cognitive function. Estimation of these models was done using Bayesian methods. Eight different outcomes representing three areas of memory functioning(visual, verbal, working) were used to define a pattern of cognitive decline. The earliest phase change was found to be visual memory(∼6 years before diagnosis) and was followed by changes in verbal and working memory beginning roughly four years later.","publication_date":{"day":null,"month":null,"year":1990,"errors":{}},"publication_name":"Ophthalmic Paediatrics and Genetics","grobid_abstract_attachment_id":88854354},"translated_abstract":null,"internal_url":"https://www.academia.edu/83546057/Red_or_rufous_albinism_in_Southern_Africa","translated_internal_url":"","created_at":"2022-07-22T02:43:46.175-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854354,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854354/thumbnails/1.jpg","file_name":"langford_ku_0099m_12512_data_1.pdf","download_url":"https://www.academia.edu/attachments/88854354/download_file","bulk_download_file_name":"Red_or_rufous_albinism_in_Southern_Afric.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854354/langford_ku_0099m_12512_data_1-libre.pdf?1658483333=\u0026response-content-disposition=attachment%3B+filename%3DRed_or_rufous_albinism_in_Southern_Afric.pdf\u0026Expires=1742176507\u0026Signature=br2~ZchkgrsPVejkBVkW2tUvvkdzFHHGKiYSpP-V1TnzkvCnFRDZijq0WdEQvoJFAA2~UB7t6Pce~GBUibzVj9fe5NbZOYSaR5gsqeqHEtJuvqWhW641IL0HHeQdXG9i-N4MBsjpmXQC1Jj~N3Lz6WGOTtw-C5mR3iGZRzhyTf6TiIwBk1YoUIn9lIgXNrfa6OO4tsZ4tN6At4ryRJiFGSO0zOCIFc5bpx6a0caXJuQHVv2~aWcZKlJK9BtV9RvjR7FZRDQGyeTpfw4~RywaRIIyfuckZeRWxY2rFexecpAvCkLn6gTyY-HLxFrQcjGhEA535f5gxGWncVsfyT5wdg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Red_or_rufous_albinism_in_Southern_Africa","translated_slug":"","page_count":38,"language":"en","content_type":"Work","summary":"As the Alzheimer's disease process progresses in time measurements of cognitive functioning exhibit nonlinearity. Multiphase models were used to quantify this nonlinearity for thirty-six well characterized individuals(∼12 observations per individual over ∼15 years in the study) by partitioning each into a healthy aging phase and a diseased phase. This enabled us to detail both the magnitude and timing that Alzheimer's disease alters different aspects of cognitive function. Estimation of these models was done using Bayesian methods. Eight different outcomes representing three areas of memory functioning(visual, verbal, working) were used to define a pattern of cognitive decline. The earliest phase change was found to be visual memory(∼6 years before diagnosis) and was followed by changes in verbal and working memory beginning roughly four years later.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854354,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854354/thumbnails/1.jpg","file_name":"langford_ku_0099m_12512_data_1.pdf","download_url":"https://www.academia.edu/attachments/88854354/download_file","bulk_download_file_name":"Red_or_rufous_albinism_in_Southern_Afric.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854354/langford_ku_0099m_12512_data_1-libre.pdf?1658483333=\u0026response-content-disposition=attachment%3B+filename%3DRed_or_rufous_albinism_in_Southern_Afric.pdf\u0026Expires=1742176507\u0026Signature=br2~ZchkgrsPVejkBVkW2tUvvkdzFHHGKiYSpP-V1TnzkvCnFRDZijq0WdEQvoJFAA2~UB7t6Pce~GBUibzVj9fe5NbZOYSaR5gsqeqHEtJuvqWhW641IL0HHeQdXG9i-N4MBsjpmXQC1Jj~N3Lz6WGOTtw-C5mR3iGZRzhyTf6TiIwBk1YoUIn9lIgXNrfa6OO4tsZ4tN6At4ryRJiFGSO0zOCIFc5bpx6a0caXJuQHVv2~aWcZKlJK9BtV9RvjR7FZRDQGyeTpfw4~RywaRIIyfuckZeRWxY2rFexecpAvCkLn6gTyY-HLxFrQcjGhEA535f5gxGWncVsfyT5wdg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":13135,"name":"Southern Africa","url":"https://www.academia.edu/Documents/in/Southern_Africa"},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent"},{"id":23267,"name":"Ophthalmic","url":"https://www.academia.edu/Documents/in/Ophthalmic"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":61866,"name":"South Africa","url":"https://www.academia.edu/Documents/in/South_Africa"},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child"},{"id":289330,"name":"Prevalence","url":"https://www.academia.edu/Documents/in/Prevalence"},{"id":349211,"name":"Skin Pigmentation","url":"https://www.academia.edu/Documents/in/Skin_Pigmentation"},{"id":359001,"name":"Optometry and Ophthalmology","url":"https://www.academia.edu/Documents/in/Optometry_and_Ophthalmology"},{"id":576640,"name":"Hair Color","url":"https://www.academia.edu/Documents/in/Hair_Color"},{"id":665851,"name":"OPHTHALMIC GENETICS","url":"https://www.academia.edu/Documents/in/OPHTHALMIC_GENETICS"},{"id":701219,"name":"Melanocytes","url":"https://www.academia.edu/Documents/in/Melanocytes"},{"id":968586,"name":"Visual Evoked Potentials","url":"https://www.academia.edu/Documents/in/Visual_Evoked_Potentials"}],"urls":[{"id":22355843,"url":"http://www.tandfonline.com/doi/pdf/10.3109/13816819009020984"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546056"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/83546056/Corticotropin_releasing_factor_A_possible_key_to_gut_dysfunction_in_the_critically_ill"><img alt="Research paper thumbnail of Corticotropin-releasing factor: A possible key to gut dysfunction in the critically ill" class="work-thumbnail" src="https://attachments.academia-assets.com/88854353/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/83546056/Corticotropin_releasing_factor_A_possible_key_to_gut_dysfunction_in_the_critically_ill">Corticotropin-releasing factor: A possible key to gut dysfunction in the critically ill</a></div><div class="wp-workCard_item"><span>Nutrition</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Critically ill patients frequently display unexplained or incompletely explained features of gast...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Critically ill patients frequently display unexplained or incompletely explained features of gastrointestinal (GI) dysfunction, including gastric stasis, ileus, and diarrhea. This makes nutrition delivery challenging, and may contribute to poor outcomes. The typical bowel dysfunction seen in severely ill patients includes retarded gastric emptying, unsynchronized intestinal motility, and intestinal hyperpermeability. These functional changes appear similar to the corticotropinreleasing factor (CRF)-mediated bowel dysfunctions associated with stress of various types and some GI disorders and diseases. CRF has been shown to be present within the GI tract and its action on CRF receptors within the gut have been shown to reduce gastric emptying, alter intestinal motility, and increase intestinal permeability. However, the precise role of CRF in the GI dysfunction in critical illness remains unclear. In this short review, we provide an update on GI dysfunction during stress and review the possible role of CRF in the aetiology of gut dysfunction. We suggest that activation of CRF signaling pathways in critical illness might be key to understanding the mechanisms underlying the gut dysfunction that impairs enteral feeding in the intensive care unit.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fe64d6ee903d5a2bf17fce1ffd01faac" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":88854353,"asset_id":83546056,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/88854353/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546056"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546056"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546056; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546056]").text(description); $(".js-view-count[data-work-id=83546056]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546056; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546056']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fe64d6ee903d5a2bf17fce1ffd01faac" } } $('.js-work-strip[data-work-id=83546056]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546056,"title":"Corticotropin-releasing factor: A possible key to gut dysfunction in the critically ill","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Critically ill patients frequently display unexplained or incompletely explained features of gastrointestinal (GI) dysfunction, including gastric stasis, ileus, and diarrhea. 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We suggest that activation of CRF signaling pathways in critical illness might be key to understanding the mechanisms underlying the gut dysfunction that impairs enteral feeding in the intensive care unit.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Nutrition","grobid_abstract_attachment_id":88854353},"translated_abstract":null,"internal_url":"https://www.academia.edu/83546056/Corticotropin_releasing_factor_A_possible_key_to_gut_dysfunction_in_the_critically_ill","translated_internal_url":"","created_at":"2022-07-22T02:43:46.076-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":88854353,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854353/thumbnails/1.jpg","file_name":"j.nut.2012.12.02320220722-1-uprmv6.pdf","download_url":"https://www.academia.edu/attachments/88854353/download_file","bulk_download_file_name":"Corticotropin_releasing_factor_A_possibl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854353/j.nut.2012.12.02320220722-1-uprmv6-libre.pdf?1658483330=\u0026response-content-disposition=attachment%3B+filename%3DCorticotropin_releasing_factor_A_possibl.pdf\u0026Expires=1742176507\u0026Signature=GtlaWMSZ7onn7y9auLQarJ9N60q57HHZIEvJ0NwYHi6GWSBBAFWpBdlw622M117haYeBlrcHUwt5hQ~b70ktcXQCg5oNKPSVfuY4yw~eN2SZf-0HhE4j6FbNM78FrCk~2~QDe5f4RyKTHkiUt4R0-zhw6hBtBX6xsZ5j2TiDcLAYf5qCdV-IpzekroO~X3asijGSJx-Rg2hsTcUFL8s3VwdQLS5D2WtRDcY2W3cTBkaXkLyewrQtvnZUlhvcBBo7~PRaFJ4CGFGj7gMcoFHtRrKumCG6k-cyHrq1FprWwCTdfKxPLZbwkoArCSw4~jDGhzMqHCezW8UFEeYortLm~g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Corticotropin_releasing_factor_A_possible_key_to_gut_dysfunction_in_the_critically_ill","translated_slug":"","page_count":5,"language":"en","content_type":"Work","summary":"Critically ill patients frequently display unexplained or incompletely explained features of gastrointestinal (GI) dysfunction, including gastric stasis, ileus, and diarrhea. This makes nutrition delivery challenging, and may contribute to poor outcomes. The typical bowel dysfunction seen in severely ill patients includes retarded gastric emptying, unsynchronized intestinal motility, and intestinal hyperpermeability. These functional changes appear similar to the corticotropinreleasing factor (CRF)-mediated bowel dysfunctions associated with stress of various types and some GI disorders and diseases. CRF has been shown to be present within the GI tract and its action on CRF receptors within the gut have been shown to reduce gastric emptying, alter intestinal motility, and increase intestinal permeability. However, the precise role of CRF in the GI dysfunction in critical illness remains unclear. In this short review, we provide an update on GI dysfunction during stress and review the possible role of CRF in the aetiology of gut dysfunction. We suggest that activation of CRF signaling pathways in critical illness might be key to understanding the mechanisms underlying the gut dysfunction that impairs enteral feeding in the intensive care unit.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[{"id":88854353,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/88854353/thumbnails/1.jpg","file_name":"j.nut.2012.12.02320220722-1-uprmv6.pdf","download_url":"https://www.academia.edu/attachments/88854353/download_file","bulk_download_file_name":"Corticotropin_releasing_factor_A_possibl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/88854353/j.nut.2012.12.02320220722-1-uprmv6-libre.pdf?1658483330=\u0026response-content-disposition=attachment%3B+filename%3DCorticotropin_releasing_factor_A_possibl.pdf\u0026Expires=1742176507\u0026Signature=GtlaWMSZ7onn7y9auLQarJ9N60q57HHZIEvJ0NwYHi6GWSBBAFWpBdlw622M117haYeBlrcHUwt5hQ~b70ktcXQCg5oNKPSVfuY4yw~eN2SZf-0HhE4j6FbNM78FrCk~2~QDe5f4RyKTHkiUt4R0-zhw6hBtBX6xsZ5j2TiDcLAYf5qCdV-IpzekroO~X3asijGSJx-Rg2hsTcUFL8s3VwdQLS5D2WtRDcY2W3cTBkaXkLyewrQtvnZUlhvcBBo7~PRaFJ4CGFGj7gMcoFHtRrKumCG6k-cyHrq1FprWwCTdfKxPLZbwkoArCSw4~jDGhzMqHCezW8UFEeYortLm~g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1907,"name":"Nutrition","url":"https://www.academia.edu/Documents/in/Nutrition"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":31276,"name":"Corticotropin Releasing Hormone","url":"https://www.academia.edu/Documents/in/Corticotropin_Releasing_Hormone"},{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction"},{"id":537759,"name":"Gastrointestinal Tract","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Tract"},{"id":722449,"name":"Physiological Stress Markers","url":"https://www.academia.edu/Documents/in/Physiological_Stress_Markers"},{"id":1548059,"name":"Critical Illness","url":"https://www.academia.edu/Documents/in/Critical_Illness"},{"id":1595009,"name":"Gastric Emptying","url":"https://www.academia.edu/Documents/in/Gastric_Emptying"},{"id":1758627,"name":"Enteral Nutrition","url":"https://www.academia.edu/Documents/in/Enteral_Nutrition"},{"id":2430299,"name":"Gastrointestinal Diseases","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Diseases"},{"id":3647007,"name":"Stress Physiological","url":"https://www.academia.edu/Documents/in/Stress_Physiological"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="83546055"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/83546055/Corticotropin_releasing_factor_is_present_in_intestinal_tissue_of_patients_with_gastrointestinal_dysfunction_following_shock_and_abdominal_surgery"><img alt="Research paper thumbnail of Corticotropin-releasing factor is present in intestinal tissue of patients with gastrointestinal dysfunction following shock and abdominal surgery" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/83546055/Corticotropin_releasing_factor_is_present_in_intestinal_tissue_of_patients_with_gastrointestinal_dysfunction_following_shock_and_abdominal_surgery">Corticotropin-releasing factor is present in intestinal tissue of patients with gastrointestinal dysfunction following shock and abdominal surgery</a></div><div class="wp-workCard_item"><span>Nutrition</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction, possibly causing gut dysfunction following trauma and surgery. We investigated plasma and intestinal tissue CRF levels and gut function in patients with traumatic shock or those undergoing elective abdominal surgery. In a prospective, parallel, observational study in a university hospital surgical intensive care unit (ICU), 8 shocked patients (systolic blood pressure &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;90 mmHg and/or acidosis and/or urine output &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 mL/kg/hr and/or requiring &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;2 L of intravenous fluid resuscitation) undergoing small bowel resection during emergency laparotomy following abdominal injury and 17 stable patients undergoing elective hepatobiliary surgery were included if they required postoperative ICU management. Serial plasma and intestinal CRF was measured and postoperative gastric emptying and intestinal permeability were evaluated. Plasma CRF was significantly increased in the shocked patients compared with the elective surgery patients at all times. CRF peptide was quantified in intestinal tissue at similar levels in both groups. Intestinal permeability was increased and associated with shock and resuscitation fluid volume. Gastric emptying was retarded and correlated significantly with shock but not with plasma CRF. Delayed gastric emptying in shocked patients was associated with longer ICU stay. The novel finding is the presence of CRF in the small bowel of both elective and emergency gastrointestinal surgery patients with concomitant gastrointestinal dysfunction. Circulating CRF is associated with poor gastric emptying, which prolongs ICU stay, whereas shock significantly impairs gastric emptying and gut permeability.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="83546055"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="83546055"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 83546055; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=83546055]").text(description); $(".js-view-count[data-work-id=83546055]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 83546055; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='83546055']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=83546055]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":83546055,"title":"Corticotropin-releasing factor is present in intestinal tissue of patients with gastrointestinal dysfunction following shock and abdominal surgery","translated_title":"","metadata":{"abstract":"Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction, possibly causing gut dysfunction following trauma and surgery. We investigated plasma and intestinal tissue CRF levels and gut function in patients with traumatic shock or those undergoing elective abdominal surgery. In a prospective, parallel, observational study in a university hospital surgical intensive care unit (ICU), 8 shocked patients (systolic blood pressure \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;90 mmHg and/or acidosis and/or urine output \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 mL/kg/hr and/or requiring \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;2 L of intravenous fluid resuscitation) undergoing small bowel resection during emergency laparotomy following abdominal injury and 17 stable patients undergoing elective hepatobiliary surgery were included if they required postoperative ICU management. Serial plasma and intestinal CRF was measured and postoperative gastric emptying and intestinal permeability were evaluated. Plasma CRF was significantly increased in the shocked patients compared with the elective surgery patients at all times. CRF peptide was quantified in intestinal tissue at similar levels in both groups. Intestinal permeability was increased and associated with shock and resuscitation fluid volume. Gastric emptying was retarded and correlated significantly with shock but not with plasma CRF. Delayed gastric emptying in shocked patients was associated with longer ICU stay. The novel finding is the presence of CRF in the small bowel of both elective and emergency gastrointestinal surgery patients with concomitant gastrointestinal dysfunction. Circulating CRF is associated with poor gastric emptying, which prolongs ICU stay, whereas shock significantly impairs gastric emptying and gut permeability.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Nutrition"},"translated_abstract":"Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction, possibly causing gut dysfunction following trauma and surgery. We investigated plasma and intestinal tissue CRF levels and gut function in patients with traumatic shock or those undergoing elective abdominal surgery. In a prospective, parallel, observational study in a university hospital surgical intensive care unit (ICU), 8 shocked patients (systolic blood pressure \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;90 mmHg and/or acidosis and/or urine output \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 mL/kg/hr and/or requiring \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;2 L of intravenous fluid resuscitation) undergoing small bowel resection during emergency laparotomy following abdominal injury and 17 stable patients undergoing elective hepatobiliary surgery were included if they required postoperative ICU management. Serial plasma and intestinal CRF was measured and postoperative gastric emptying and intestinal permeability were evaluated. Plasma CRF was significantly increased in the shocked patients compared with the elective surgery patients at all times. CRF peptide was quantified in intestinal tissue at similar levels in both groups. Intestinal permeability was increased and associated with shock and resuscitation fluid volume. Gastric emptying was retarded and correlated significantly with shock but not with plasma CRF. Delayed gastric emptying in shocked patients was associated with longer ICU stay. The novel finding is the presence of CRF in the small bowel of both elective and emergency gastrointestinal surgery patients with concomitant gastrointestinal dysfunction. Circulating CRF is associated with poor gastric emptying, which prolongs ICU stay, whereas shock significantly impairs gastric emptying and gut permeability.","internal_url":"https://www.academia.edu/83546055/Corticotropin_releasing_factor_is_present_in_intestinal_tissue_of_patients_with_gastrointestinal_dysfunction_following_shock_and_abdominal_surgery","translated_internal_url":"","created_at":"2022-07-22T02:43:45.773-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":212799908,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Corticotropin_releasing_factor_is_present_in_intestinal_tissue_of_patients_with_gastrointestinal_dysfunction_following_shock_and_abdominal_surgery","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Corticotropin-releasing factor (CRF) is implicated in stress-related gastrointestinal dysfunction, possibly causing gut dysfunction following trauma and surgery. We investigated plasma and intestinal tissue CRF levels and gut function in patients with traumatic shock or those undergoing elective abdominal surgery. In a prospective, parallel, observational study in a university hospital surgical intensive care unit (ICU), 8 shocked patients (systolic blood pressure \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;90 mmHg and/or acidosis and/or urine output \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 mL/kg/hr and/or requiring \u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;2 L of intravenous fluid resuscitation) undergoing small bowel resection during emergency laparotomy following abdominal injury and 17 stable patients undergoing elective hepatobiliary surgery were included if they required postoperative ICU management. Serial plasma and intestinal CRF was measured and postoperative gastric emptying and intestinal permeability were evaluated. Plasma CRF was significantly increased in the shocked patients compared with the elective surgery patients at all times. CRF peptide was quantified in intestinal tissue at similar levels in both groups. Intestinal permeability was increased and associated with shock and resuscitation fluid volume. Gastric emptying was retarded and correlated significantly with shock but not with plasma CRF. Delayed gastric emptying in shocked patients was associated with longer ICU stay. The novel finding is the presence of CRF in the small bowel of both elective and emergency gastrointestinal surgery patients with concomitant gastrointestinal dysfunction. Circulating CRF is associated with poor gastric emptying, which prolongs ICU stay, whereas shock significantly impairs gastric emptying and gut permeability.","owner":{"id":212799908,"first_name":"Susan","middle_initials":null,"last_name":"KIDSON","page_name":"KIDSONS","domain_name":"independent","created_at":"2022-01-20T15:02:27.893-08:00","display_name":"Susan KIDSON","url":"https://independent.academia.edu/KIDSONS"},"attachments":[],"research_interests":[{"id":1907,"name":"Nutrition","url":"https://www.academia.edu/Documents/in/Nutrition"},{"id":31276,"name":"Corticotropin Releasing Hormone","url":"https://www.academia.edu/Documents/in/Corticotropin_Releasing_Hormone"},{"id":55155,"name":"Emergency Medical Services","url":"https://www.academia.edu/Documents/in/Emergency_Medical_Services"},{"id":211917,"name":"Length of Stay","url":"https://www.academia.edu/Documents/in/Length_of_Stay"},{"id":288383,"name":"Intestinal absorption","url":"https://www.academia.edu/Documents/in/Intestinal_absorption"},{"id":291136,"name":"Intestines","url":"https://www.academia.edu/Documents/in/Intestines"},{"id":550383,"name":"Gastrointestinal motility","url":"https://www.academia.edu/Documents/in/Gastrointestinal_motility"},{"id":647820,"name":"Fluid Therapy","url":"https://www.academia.edu/Documents/in/Fluid_Therapy"},{"id":897823,"name":"Elsevier","url":"https://www.academia.edu/Documents/in/Elsevier"},{"id":1595009,"name":"Gastric Emptying","url":"https://www.academia.edu/Documents/in/Gastric_Emptying"},{"id":1653983,"name":"Abdomen","url":"https://www.academia.edu/Documents/in/Abdomen"},{"id":2430299,"name":"Gastrointestinal Diseases","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Diseases"},{"id":2463621,"name":"Postoperative Complications","url":"https://www.academia.edu/Documents/in/Postoperative_Complications"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"},{"id":3834135,"name":"abdominal Injuries","url":"https://www.academia.edu/Documents/in/abdominal_Injuries"}],"urls":[]}, dispatcherData: dispatcherData }); 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