Cell Science Tools & Funding - Chan Zuckerberg Initiative

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class="container--global-search"> <div class="container--search-items"> <form class="search" method="get" action="https://chanzuckerberg.com" role="search" > <div class="search-icon"> <svg xmlns="http://www.w3.org/2000/svg" width="23" height="23" viewBox="0 0 23 23" fill="none" > <style> .c { stroke-width: 2; stroke: #ff414b; } </style> <path d="M15.5 15.7L21.4 21.6" class="c" /> <path d="M9.7 18.1C14.2 18.1 17.9 14.4 17.9 9.9 17.9 5.3 14.2 1.6 9.7 1.6 5.1 1.6 1.4 5.3 1.4 9.9 1.4 14.4 5.1 18.1 9.7 18.1Z" class="c" /> </svg> </div> <input class="search--textfield" type="text" name="s" id="search" value="" placeholder="Search" aria-label="Search" /> <button type="submit"> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </button> </form> <div class="close-icon"> <svg xmlns="http://www.w3.org/2000/svg" width="44" height="44" viewBox="0 0 44 44" fill="none" > <style> .b { stroke-width: 2; stroke: black; } </style> <circle cx="22" cy="22" r="22" fill="#FAFAFA" /> <g opacity="0.8"> <path d="M15.1 14.9L29.2 29.1" class="b" /> <path d="M15.1 29.1L29.2 14.9" class="b" /> </g> </svg> </div> </div> </div> </div> </header> <main id="content" role="main" class="content-wrapper with-subnav ">  <div class="search-overlay "></div> <div class="dimmed-overlay"></div> <section class="section--hero hero--2-3"> <div class="hero__wrapper section--border-bottom"> <div class="hero__bkg"></div> <div class="hero__block"> <h1 class="hero__title t-h2--bold color--red">Cell Science</h1> <div class="t-body--large section--border-txt-left"> <p>The CZI Cell Science program (formerly the Single-Cell Biology program) supports and enables the generation and application of technologies for the engineering and research of complex cellular systems. Despite an ever-increasing knowledge of cell types and states, the vast majority of primary cells remain remarkably difficult to culture and experiment with. The Cell Science program seeks to change this by building on their established interdisciplinary collaborations to derive molecular definitions of cells and dig deeper into their biology. At its core, the program is focused on scalable technology that enables deeper insight into cells in health and disease states via experimentation and modeling.</p> </div> </div> </div> <div class="hero__wrapper section--border-bottom"> <div class="hero__block--img"> <div class="hero__image hero__image--2-3-first frame frame--red"> <div class="fit-photo fit-photo "> <img src="https://chanzuckerberg.com/wp-content/uploads/2020/04/immunofluorescence-image-of-the-small-intestine-emma-lundberg-1.jpg" alt="An immunofluorescence image of the first part of the small intestine, called the duodenum (CZI Single-Cell Biology)." object-fit-polyfill /> <div class="caption--overlay t-caption "></div> <figcaption class="caption t-caption "> An immunofluorescence image of the first part of the small intestine, called the duodenum. Photo provided by Emma Lundberg, KTH Royal Institute of Technology, Sweden.</figcaption> </div> <div class="caption--overlay t-caption is-left color--white "></div> <figcaption class="caption t-caption is-left color--white "> An immunofluorescence image of the first part of the small intestine, called the duodenum. Photo provided by Emma Lundberg, KTH Royal Institute of Technology, Sweden.</figcaption> </div> </div> </div> </section> <div class="funded-module"> <div class="funded-module--title"> <h6 class="t-h6--semibold">The Cell Science program has funded the following RFAs:</h6> </div> <div class="funded-module--col"> <div class="funded-module--col-left"> <div class="funded-module--col-left-content"> <ul> <li><a href="https://chanzuckerberg.com/rfa/measuring-metabolism-across-scales/"><span style="font-weight: 400">Measuring Metabolism Across Scales</span></a>, $8.6 million</li> <li><span style="font-weight: 400">Single-Cell Biology Data Insights (</span><a href="https://chanzuckerberg.com/science/programs-resources/cell-science/data-insights/?cycle=1"><span style="font-weight: 400">Cycle 1</span></a><span style="font-weight: 400">, $5.2 million and </span><a href="https://chanzuckerberg.com/science/programs-resources/cell-science/data-insights/?cycle=2"><span style="font-weight: 400">Cycle 2</span></a><span style="font-weight: 400">, $4.9 million, <a href="https://chanzuckerberg.com/science/programs-resources/cell-science/data-insights/?cycle=3">Cycle 3</a> $4.8 million)</span></li> <li><a href="https://chanzuckerberg.com/patient-partnered-collaborations-for-single-cell-analysis-of-rare-inflammatory-pediatric-disease-rfa-grantees/"><span style="font-weight: 400">Patient-Partnered Single-Cell Analysis of Rare Pediatric Disease</span></a><span style="font-weight: 400">, $10 million</span></li> </ul> </div> </div> <div class="funded-module--col-right"> <div class="funded-module--col-right-content"> <ul> <li><a href="https://chanzuckerberg.com/science/programs-resources/cell-science/ancestry-networks/"><span style="font-weight: 400">Ancestry Networks for the Human Cell Atlas</span></a><span style="font-weight: 400">, $27.9 million</span></li> <li><a href="https://chanzuckerberg.com/science/programs-resources/cell-science/pediatric-networks/"><span style="font-weight: 400">Pediatric Networks for the Human Cell Atlas</span></a><span style="font-weight: 400">, $34.2 million</span></li> <li><a href="https://chanzuckerberg.com/science/programs-resources/cell-science/inflammation-projects/"><span style="font-weight: 400">Single-Cell Analysis of Inflammation</span></a><span style="font-weight: 400">, $14 million</span></li> <li><a href="https://chanzuckerberg.com/science/programs-resources/cell-science/seednetworks/"><span style="font-weight: 400">Seed Networks for the Human Cell Atlas</span></a><span style="font-weight: 400">, $67.7 million</span></li> <li><a href="https://chanzuckerberg.com/human-cell-atlas-collab/"><span style="font-weight: 400">Collaborative Computational Tools for a Human Cell Atlas</span></a><span style="font-weight: 400">, $14.8 million</span></li> <li><a href="https://chanzuckerberg.com/human-cell-atlas-pilot-projects/"><span style="font-weight: 400">Pilot Projects for a Human Cell Atlas</span></a><span style="font-weight: 400">, $14.9 million</span></li> </ul> </div> </div> </div> </div> <div class="header--rao-cards content__centered"> <h2 class="t-h2--semibold color--red">Single-Cell Technology</h1> </div> <section class="section--intro"> <div class="section--intro__container"> <div class="section--intro__container-body"> <p>We build tools to support and accelerate the work of biologists who are uncovering new insights about healthy and diseased cells. Our platform, <a href="https://cellxgene.cziscience.com/" target="_blank" rel="noopener">Chan Zuckerberg CELL by GENE</a> (CZ CELLxGENE), allows scientists to access, analyze, and annotate high-dimensional single-cell data at scale, so they can inform their experiments or quickly surface new insights that could lead to discoveries in treating disease. Learn more at <a href="https://cellxgene.cziscience.com/" target="_blank" rel="noopener">czcellxgene.org</a> and read our <a href="https://www.biorxiv.org/content/10.1101/2023.10.30.563174v1" target="_blank" rel="noopener">pre-print</a>.</p> </div> <div class="section--intro__rows"> <div class="section--intro__rowItem"> <div class="section--intro__rowItem-showcase"> <img src="https://chanzuckerberg.com/wp-content/uploads/2023/11/cellxgene-discover-logo.png" alt="" /> <div> <h3 class="section--intro__rowItem-title t-body--large">CZ CELL×GENE Discover</h3> <p><span style="font-weight: 300">CZ CELLxGENE Discover provides scientists with fast, easy access to the largest collection of standardized single-cell data — 50+ million cells and growing. Scientists can access the data in different ways based on their research needs:</span></p> </div> </div> <div class="section--intro__rowItem-cardGrid"> <div class="section--intro__rowItem-card"> <img src="https://chanzuckerberg.com/wp-content/uploads/2023/11/cellxgene-discover-census.png" alt="" /> <div class="section--intro__rowItem-cardDescription"> <h4>Census</h4> <p>Access any custom slice of standardized cell data available on CZ CELLxGENE Discover via R and Python. <br><br><a href="https://chanzuckerberg.github.io/cellxgene-census/" target="_blank"><b>Learn More</b></a></p> </div> </div> <div class="section--intro__rowItem-card"> <img src="https://chanzuckerberg.com/wp-content/uploads/2023/11/cellxgene-discover-cellguide.png" alt="" /> <div class="section--intro__rowItem-cardDescription"> <h4>CellGuide</h4> <p>Explore an interactive encyclopedia of 700+ cell types that provides detailed definitions, marker genes, lineage, and relevant datasets in one place.<br><br><a href="https://cellxgene.cziscience.com/cellguide" target="_blank"><b>Learn More</b></a></p> </div> </div> <div class="section--intro__rowItem-card"> <img src="https://chanzuckerberg.com/wp-content/uploads/2023/11/cellxgene-discover-geneexpression.png" alt="" /> <div class="section--intro__rowItem-cardDescription"> <h4>Gene Expression</h4> <p>Uncover expression levels of any gene across datasets of cell types and tissues with advanced visualization tools. <br><br><a href="https://cellxgene.cziscience.com/gene-expression" target="_blank"><b>Learn More</b></a></p> </div> </div> </div> </div> <div class="section--intro__rowItem"> <div class="section--intro__rowItem-showcase"> <img src="https://chanzuckerberg.com/wp-content/uploads/2023/11/cellxgene-annotate-logo.png" alt="" /> <div> <h3 class="section--intro__rowItem-title t-body--large">CZ CELL×GENE Annotate</h3> <p>CZ CELLxGENE Annotate is a self-hosting interactive data explorer for single-cell datasets. Our desktop tool is a powerful interface that allows you to annotate, analyze, and explore your own single-cell data. Learn more <a href="https://cellxgene.cziscience.com/docs/05__Annotate%20and%20Analyze%20Your%20Data/5_0__Get%20Started" target="_blank" rel="noopener">here</a>.</p> </div> </div> </div> </div> </div> </section> <div class="header--rao-cards content__centered"> <h2 class="t-h2--semibold color--red">Cellular Measurement</h1> </div> <section class="section--intro"> <div class="section--intro__container"> <div class="section--intro__container-body"> <p>Advancements in technology offer an unprecedented potential to profile and visualize cells, RNA, proteins, and metabolites. This work aims to generate comprehensive data references spanning modalities, ancestries, and developmental stages that can be used to rapidly understand the molecular mechanisms that define cells.</p> </div> </div> </section> <section class="section--cards-grid section--padding-t-2 section--strategic-grid carousel cards-grid--carousel cards-grid--related cards-grid--highlights-carousel" carousel-container data-number-moving="1" data-stop-offset-mobile="0" data-stop-offset="2" id="" > <div class="carousel__slider highlights-carousel section-- " carousel-slider> <div class="cards-section cards-section--related cards-section--3-cols section--"> <div class="card hover-single-card list__row is-active" carousel-item card> <a href="https://chanzuckerberg.com/science/programs-resources/cell-science/ancestry-networks/" class="hover-card hover-single-card bg--pink" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2021/11/20180514HumanBPHDonor20KRT5KRT13KRT8-Doug-Strand.jpeg" alt="" object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--pink"></div> <div class="hover-card__copy"> <h4 class="t-h5--semibold t--hc"> Ancestry Networks for the Human Cell Atlas </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--pink"></div> <p class="t-body--x-small"> These projects support researchers to contribute healthy, single-cell reference data from tissue samples from ancestrally diverse donors to the Human Cell Atlas, with the aim of creating a more globally representative resource to understand disease. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title"></p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--pink"> <span class="color--black"> Ancestry Networks for the Human Cell Atlas </span> </div> </a> </div> <div class="card hover-single-card list__row is-active" carousel-item card> <a href="https://chanzuckerberg.com/science/programs-resources/cell-science/pediatric-networks/" class="hover-card hover-single-card bg--blue" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2021/08/pediatrics-1.jpeg" alt="Pediatric Networks for the Human Cell Atlas photo of nine cells (CZI Single-Cell Biology)." object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--blue"></div> <div class="hover-card__copy"> <h4 class="t-h5--semibold t--hc"> Pediatric Networks for the Human Cell Atlas </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--blue"></div> <p class="t-body--x-small"> These projects support researchers to contribute pediatric tissue samples to the Human Cell Atlas as a foundation for understanding how cells and organs develop and relate to disease onset in children. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title"></p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--blue"> <span class="color--black"> Pediatric Networks for the Human Cell Atlas </span> </div> </a> </div> <div class="card hover-single-card list__row is-active" carousel-item card> <a href="https://chanzuckerberg.com/science/programs-resources/cell-science/seednetworks/" class="hover-card hover-single-card bg--red" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2020/04/human-tendon-cells-scaffolds-sarah-snelling-1.jpg" alt="Seed Networks for the Human Cell Atlas photo of human tendon cell scaffolds (CZI Single-Cell Biology)." object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--red"></div> <div class="hover-card__copy"> <h4 class="t-h5--semibold t--hc"> Seed Networks </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--red"></div> <p class="t-body--x-small"> The projects below bring together experimental scientists, computational biologists, software engineers, and physicians to support the development of the first draft Human Cell Atlas. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title"></p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--red"> <span class="color--black"> Seed Networks </span> </div> </a> </div> <div class="card hover-single-card list__row is-active" carousel-item card> <a href="https://chanzuckerberg.com/human-cell-atlas-pilot-projects/" class="hover-card hover-single-card bg--yellow" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2020/04/poster-session-pilot-projectss-human-cell-atlas-1.jpeg" alt="Pilot Projects photo of people with nametags standing at a poster session, paired together and engaging in discussions (CZI Single-Cell Biology)." object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--yellow"></div> <div class="hover-card__copy"> <h4 class="t-h5--semibold t--hc"> Pilot Projects </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--yellow"></div> <p class="t-body--x-small"> 38 projects to help build new technologies, best practices, and data analysis techniques. The projects delivered technology improvements and benchmarking to help international scientists determine optimal protocols and methods for atlas generation. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title"></p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--yellow"> <span class="color--black"> Pilot Projects </span> </div> </a> </div> <div class="card hover-single-card list__row is-active" carousel-item card> <a href="https://chanzuckerberg.com/science/programs-resources/cell-science/metabolism/" class="hover-card hover-single-card bg--teal" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2023/10/cortical-organoid-3-1.jpg" alt="" object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--teal"></div> <div class="hover-card__copy"> <h4 class="t-h5--semibold t--hc"> Measuring Metabolism Across Scales </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--teal"></div> <p class="t-body--x-small"> These projects focus on measuring metabolism across organelles and cells to accelerate innovative discoveries in metabolism and metabolic physiology in health and disease. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title"></p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--teal"> <span class="color--black"> Measuring Metabolism Across Scales </span> </div> </a> </div> </div> </div> <div class="carousel__controls" carousel-controls> <div class="carousel__controls-inner"> <button class="button-carousel button-prev" aria-label="previous" carousel-prev><div class="cta-arrow"></div></button> <button class="button-carousel button-next" aria-label="next" carousel-next><div class="cta-arrow"></div></button> </div> <span class="carousel__index-number t-body--medium mobile-only"> <span class="carousel__index-number"><span carousel-active-index></span> / 5</span> </span> </div> </section> <div class="header--rao-cards content__centered"> <h2 class="t-h2--semibold color--red">Prediction Engines</h2> </div> <section class="section--intro"> <div class="section--intro__container"> <div class="section--intro__container-body"> <p>There remain many opportunities to use and reuse single-cell data to more deeply interrogate cellular mechanisms and disease. This work aims to equip leading-edge computational scientists with large data resources and unlock untapped power to describe cellular function.</p> </div> </div> </section> <section class="section--cards-grid section--cs-hover-cards " id="" > <div class="cards-section cards-section-- cards-section--2-cols-1-2 section--"> <div class="card hover-single-card list__row is-active" card> <a href="/human-cell-atlas-collab/" class="hover-card hover-single-card bg--yellow" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/04/Collaborative-Computational-Tools-for-a-Human-Cell-Atlas.jpg" alt="" object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--yellow"></div> <div class="hover-card__copy"> <h4 class="t-h6--semibold t--hc"> Collaborative Computational Tools for a Human Cell Atlas </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--yellow"></div> <p class="t-body--x-small"> 85 projects to develop computational technology, extend foundational single-cell analysis tools, algorithms, visualizations, and benchmark datasets. This program sought to bring together computational researchers working with single-cell data but also encourage experts in domains such as machine learning, applied physics and modeling to work on cell-based measurements. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title">Collaborative Computational Tools for a Human Cell Atlas</p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--yellow"> <span class="color--black"> Collaborative Computational Tools for a Human Cell Atlas </span> </div> </a> </div> <div class="card hover-single-card list__row is-active" card> <a href="/science/programs-resources/cell-science/data-insights/" class="hover-card hover-single-card bg--blue" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/04/Single-Cell-Biology-Data-Insights.jpg" alt="" object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--blue"></div> <div class="hover-card__copy"> <h4 class="t-h6--semibold t--hc"> Single-Cell Biology Data Insights </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--blue"></div> <p class="t-body--x-small"> These projects support researchers and computational experts to perform meta-analyses, advance computational tools, and develop cutting edge methods and resources that make it possible to gain greater insights into health and disease from existing single-cell biology datasets. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title">Single-Cell Biology Data Insights</p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--blue"> <span class="color--black"> Single-Cell Biology Data Insights </span> </div> </a> </div> </div></section> <div class="header--rao-cards content__centered"> <h2 class="t-h2--semibold color--red">Perturbation and Validation</h2> </div> <section class="section--intro"> <div class="section--intro__container"> <div class="section--intro__container-body"> <p>Perturbation and manipulation of complex cellular systems are critical to extending and validating inferences made by current cell atlases. This work focuses on the development of new technologies that enable scientists to perturb cells and systems at scale across organisms, modalities, and diseases.</p> </div> </div> </section> <section class="section--cards-grid section--cs-hover-cards " id="" > <div class="cards-section cards-section-- cards-section--2-cols-1-2 section--"> <div class="card hover-single-card list__row is-active" card> <a href="/patient-partnered-collaborations-for-single-cell-analysis-of-rare-inflammatory-pediatric-disease-rfa-grantees/" class="hover-card hover-single-card bg--green" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/04/Partnered.jpg" alt="" object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--green"></div> <div class="hover-card__copy"> <h4 class="t-h6--semibold t--hc"> Patient-Partnered Single-Cell Analysis of Rare Pediatric Disease </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--green"></div> <p class="t-body--x-small"> These projects support applying single-cell biology methods to rare inflammatory pediatric diseases to clarify cellular mechanisms of disease, understand disease heterogeneity, and improve biomarker identification or diagnostics. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title">Patient-Partnered Single-Cell Analysis of Rare Pediatric Disease</p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--green"> <span class="color--black"> Patient-Partnered Single-Cell Analysis of Rare Pediatric Disease </span> </div> </a> </div> <div class="card hover-single-card list__row is-active" card> <a href="/science/programs-resources/cell-science/inflammation-projects/" class="hover-card hover-single-card bg--pink" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/04/Single-Cell-Analysis-of-Inflammation.jpg" alt="" object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--pink"></div> <div class="hover-card__copy"> <h4 class="t-h6--semibold t--hc"> Single-Cell Analysis of Inflammation </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--pink"></div> <p class="t-body--x-small"> Inflammation is a natural defense that helps our bodies maintain a healthy state, but chronic inflammation results in harmful diseases like asthma, arthritis, and heart disease. We fund scientists to study the role of inflammation in maintaining health and triggering diseases so that we can better diagnose, treat, and manage them. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title">Single-Cell Analysis of Inflammation</p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--pink"> <span class="color--black"> Single-Cell Analysis of Inflammation </span> </div> </a> </div> </div></section> <div class="header--rao-cards content__centered"> <h2 class="t-h2--semibold color--red">Cell Engineering</h2> </div> <section class="section--intro"> <div class="section--intro__container"> <div class="section--intro__container-body"> <p>This work moves beyond functional interrogation and extends into engineering cell identity, fate, or behavior using synthetic biology, with the goal of validating our molecular knowledge of cells.</p> </div> </div> </section> <section class="section--cards-grid section--cs-hover-cards " id="" > <div class="cards-section cards-section-- cards-section--2-cols-1-2 section--"> <div class="card hover-single-card list__row is-active" card> <a href="/newsroom/seattle-hub-for-synthetic-biology-launches/" class="hover-card hover-single-card bg--blue" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/04/Seattle-Hub-for-Synthetic-Biology.jpg" alt="" object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--blue"></div> <div class="hover-card__copy"> <h4 class="t-h6--semibold t--hc"> Seattle Hub for Synthetic Biology </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--blue"></div> <p class="t-body--x-small"> The Seattle Hub for Synthetic Biology is a first-of-its-kind research initiative that will develop technologies to reveal how changes in cells and genes over time influence human health and disease. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title">Seattle Hub for Synthetic Biology</p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--blue"> <span class="color--black"> Seattle Hub for Synthetic Biology </span> </div> </a> </div> <div class="card hover-single-card list__row is-active" card> <a href="/science/programs-resources/cell-science/single-cell-exploratory-cell-networks-projects/" class="hover-card hover-single-card bg--yellow" target="" > <div class="hover-card__inner"> <div class="fit-photo"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/04/Exploratory-Cell-Networks.jpg" alt="" object-fit-polyfill /> </div> <div class="hover-card__content"> <div class="hover-card__bg background--yellow"></div> <div class="hover-card__copy"> <h4 class="t-h6--semibold t--hc"> Exploratory Cell Networks </h4> <span class="t-smallcaps-plain--small" ></span > <div class="hover-card__dash background--yellow"></div> <p class="t-body--x-small"> These multi-institution projects will develop technologies to engineer cells and tissues by applying cell biology, scalable measurement of molecules, and the application of synthetic biology methods to explore poorly understood interactions in the cell from metabolites, proteins, organelles to tissue to better understand their role in health and disease. </p> <div class="cta--block"> <span class="hover-card__cta t-smallcaps-plain--small"> <p class="cta--title">Exploratory Cell Networks</p> <div class="button-cta__arrow cta-arrow cta-arrow--red"> <div class="cta-arrow__bkg"></div> </div> </span> </div> </div> </div> </div> <div class="hover-card__hover-title background--yellow"> <span class="color--black"> 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t-body--x-small td-searchable"> - $14.9 Million</span> </li> </ul> </div> </div> </div> </div> <div class="card rfa-card list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/rfa/measuring-metabolism-across-scales/" class="card__link" title="Visit Measuring Metabolism Across Scales" aria-labelledby="card-title-27179"></a> <div class="card__pattern background--pink"> <svg viewBox="0 0 315 315" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><clipPath id="a"><path d="m0 0h315v315h-315z"/></clipPath><g style="clip-path:url(#a);opacity:0.35;fill:none;stroke:black;stroke-linecap:round;stroke-miterlimit:10"><path d="m-77.51-77.51h470.02v470.02h-470.02z" transform="matrix(.70710678 -.70710678 .70710678 .70710678 -65.24 157.5)"/><path d="m-64.34-64.34h443.68v443.68h-443.68z" transform="matrix(.70710678 -.70710678 .70710678 .70710678 -65.24 157.5)"/><path d="m-51.17-51.17h417.34v417.34h-417.34z" 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class="card__alert t-smallcaps-plain--large background--gray-dark">Closed</div> <div class="card__header"> <h5 class="card__surtitle t-smallcaps-plain--small color--red">Request for Applications</h5> <h5 class="card__surtitle t-smallcaps-plain--small color--off-black td-searchable">Cell Science</h5> <h4 class="card__title t-h5--semibold td-searchable" id="card-title-27179">Measuring Metabolism Across Scales</h4> </div> <p class="card__text t-body--x-small td-searchable"> This RFA aims to accelerate innovative discoveries in the field of metabolism and metabolic physiology in health and disease. </p> <div class="card__footer"> <ul class="card__infos"> <li> <span class="card__info__title color--red t-body--x-small">Total Grant Resources </span> <span class="card__info__desc t-body--x-small td-searchable">$8.6 Million</span> </li> </ul> <a href="https://chanzuckerberg.com/rfa/measuring-metabolism-across-scales/" target="_self" class="button-cta button-cta-- button-cta-- " title="Visit Measuring Metabolism Across Scales page" aria-label="" > <div class="button-cta__text " >RFA Details</div> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </a> </div> </div> </div> </div> <div class="card rfa-card list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/rfa/patient-partnered-collaborations-ppc-for-single-cell-analysis-of-rare-inflammatory-pediatric-disease/" class="card__link" title="Visit Patient-Partnered Single-Cell Analysis of Rare Pediatric Disease RFA" aria-labelledby="card-title-21196"></a> <div class="card__pattern background--pink"> <svg viewBox="0 0 315 315" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><clipPath id="a"><path d="m0 0h315v315h-315z"/></clipPath><g style="clip-path:url(#a);opacity:0.35;fill:none;stroke:black;stroke-linecap:round;stroke-miterlimit:10"><rect height="470.02" rx="235.01" transform="matrix(.70710678 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td-searchable" id="card-title-21196">Patient-Partnered Single-Cell Analysis of Rare Pediatric Disease RFA</h4> </div> <p class="card__text t-body--x-small td-searchable"> This RFA aims to support the application of single-cell biology methods to rare inflammatory pediatric diseases that will clarify cellular mechanisms of disease, understand disease heterogeneity, and improve biomarker identification or diagnostics. </p> <div class="card__footer"> <ul class="card__infos"> <li> <span class="card__info__title color--red t-body--x-small">Total Grant Resources </span> <span class="card__info__desc t-body--x-small td-searchable">$10 Million</span> </li> </ul> <a href="https://chanzuckerberg.com/rfa/patient-partnered-collaborations-ppc-for-single-cell-analysis-of-rare-inflammatory-pediatric-disease/" target="_self" class="button-cta button-cta-- button-cta-- " title="Visit Patient-Partnered Single-Cell Analysis of Rare Pediatric Disease RFA page" aria-label="" > <div class="button-cta__text " >RFA Details</div> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </a> </div> </div> </div> </div> </div> <div class="cards-section cards-section--carousel cards-section--3-cols section--border-top"> <div class="card rfa-card list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/rfa/ancestry-networks-human-cell-atlas/" class="card__link" title="Visit Ancestry Networks for the Human Cell Atlas" aria-labelledby="card-title-15450"></a> <div class="card__pattern background--pink"> <svg viewBox="0 0 315 315" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><clipPath id="a"><path d="m0 0h315v315h-315z"/></clipPath><g style="clip-path:url(#a);opacity:0.35;fill:none;stroke:black;stroke-linecap:round;stroke-miterlimit:10"><path d="m-77.51-77.51h470.02v470.02h-470.02z" transform="matrix(.70710678 -.70710678 .70710678 .70710678 -65.24 157.5)"/><path 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color--red t-body--x-small">Total Grant Resources </span> <span class="card__info__desc t-body--x-small td-searchable">$28 Million</span> </li> </ul> <a href="https://chanzuckerberg.com/rfa/ancestry-networks-human-cell-atlas/" target="_self" class="button-cta button-cta-- button-cta-- " title="Visit Ancestry Networks for the Human Cell Atlas page" aria-label="" > <div class="button-cta__text " >RFA Details</div> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </a> </div> </div> </div> </div> <div class="card rfa-card list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/rfa/single-cell-pediatrics/" class="card__link" title="Visit Pediatric Networks for the Human Cell Atlas" aria-labelledby="card-title-14646"></a> <div class="card__pattern background--teal"> <svg viewBox="0 0 315 315" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><clipPath id="a"><path 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t-smallcaps-plain--small color--red">Request for Applications</h5> <h5 class="card__surtitle t-smallcaps-plain--small color--off-black td-searchable">Cell Science</h5> <h4 class="card__title t-h5--semibold td-searchable" id="card-title-14646">Pediatric Networks for the Human Cell Atlas</h4> </div> <p class="card__text t-body--x-small td-searchable"> This grant program supports researchers to contribute pediatric tissue samples to the global Human Cell Atlas as a foundation for understanding how cells and organs progress and relate to disease onset in children. </p> <div class="card__footer"> <ul class="card__infos"> <li> <span class="card__info__title color--red t-body--x-small">Total Grant Resources </span> <span class="card__info__desc t-body--x-small td-searchable">$33 million</span> </li> </ul> <a href="https://chanzuckerberg.com/rfa/single-cell-pediatrics/" target="_self" class="button-cta button-cta-- button-cta-- " title="Visit Pediatric Networks for the Human Cell Atlas page" aria-label="" > <div class="button-cta__text " >RFA Details</div> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </a> </div> </div> </div> </div> <div class="card rfa-card list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/rfa/single-cell-analysis-inflammation/" class="card__link" title="Visit Single-Cell Analysis of Inflammation" aria-labelledby="card-title-6257"></a> <div class="card__pattern background--teal"> <svg viewBox="0 0 315 315" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><clipPath id="a"><path d="m0 0h315v315h-315z"/></clipPath><g style="clip-path:url(#a);opacity:0.35;fill:none;stroke:black;stroke-linecap:round;stroke-miterlimit:10"><path d="m-77.51-77.51h470.02v470.02h-470.02z" transform="matrix(.70710678 -.70710678 .70710678 .70710678 -65.24 157.5)"/><path d="m-64.34-64.34h443.68v443.68h-443.68z" transform="matrix(.70710678 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class="card__background background--off-gray"></div> <div class="card__container"> <div class="card__alert t-smallcaps-plain--large background--gray-dark">Closed</div> <div class="card__header"> <h5 class="card__surtitle t-smallcaps-plain--small color--red">Request for Applications</h5> <h5 class="card__surtitle t-smallcaps-plain--small color--off-black td-searchable">Cell Science</h5> <h4 class="card__title t-h5--semibold td-searchable" id="card-title-6257">Single-Cell Analysis of Inflammation</h4> </div> <p class="card__text t-body--x-small td-searchable"> This RFA supports interdisciplinary scientists as they apply new tools and work together in accelerating inflammation research. 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class="card__infos"> <li> <span class="card__info__title color--red t-body--x-small">Total Grant Resources </span> <span class="card__info__desc t-body--x-small td-searchable">$15 million </span> </li> </ul> <a href="https://chanzuckerberg.com/rfa/pilot-projects-for-a-human-cell-atlas/" target="_self" class="button-cta button-cta-- button-cta-- " title="Visit Pilot Projects for a Human Cell Atlas page" aria-label="" > <div class="button-cta__text " >RFA Details</div> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </a> </div> </div> </div> </div> </div> </div> <footer class="carousel__footer"> <div class="carousel__buttons" carousel-controls> <button tabindex="-1" class="button-carousel button-prev" aria-label="prev" carousel-prev><div class="cta-arrow"></div></button> <button tabindex="-1" class="button-carousel button-next" aria-label="next" carousel-next><div class="cta-arrow"></div></button> </div> <span 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Get the latest information from the links below.</p> </header> <div class="carousel__slider highlights-carousel section-- " carousel-slider> <div class="cards-section cards-section--latest cards-section--3-cols section--"> <div class="card background-image-card card--align-top card--align-center card--button-bottom list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/blog/series/advancing-science-ai/" class="card__link" title="Explore Series - Advancing Scientific Discovery With AI" aria-labelledby="card-title-33251"></a> <div class="card__background "> <div class="fit-photo fit-photo "> <picture> <source media="(max-width: 768px)" srcset="https://chanzuckerberg.com/wp-content/uploads/2024/11/24_11-CZI-BlogSeries-Advancing-Scientific-Discovery-with-AI-Mobile-BLANK.jpg"> <source media="(min-width: 769px)" srcset="https://chanzuckerberg.com/wp-content/uploads/2024/11/24_11-CZI-BlogSeries-Advancing-Scientific-Discovery-with-AI-Desktop-BLANK.jpg"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/11/24_11-CZI-BlogSeries-Advancing-Scientific-Discovery-with-AI-Desktop-BLANK.jpg" alt="A colorful microscopic image shows cellular structures in shades of blue and green, illustrating cellular imaging work." object-fit-polyfill /> </picture> </div> </div> <div class="card__container"> <div class="card__surtitle t-category-label background--green color--white">Science & Technology</div> <div class="card__title t-h4--bold color--white" id="card-title-33251">Advancing Scientific Discovery With AI</div> <a href="https://chanzuckerberg.com/blog/series/advancing-science-ai/" target="_self" class="button-cta button-cta-- button-cta--light-fill " title="Explore Series" aria-label="" > <div class="button-cta__text " >Explore Series</div> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </a> </div> </div> </div> <div class="card background-image-card card--align-top card--align-center card--button-bottom list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/blog/priscilla-chan-update-2024-biohub-progress/" class="card__link" title="Read Story - How Biohub Collaboration Unraveled a Medical Mystery" aria-labelledby="card-title-32924"></a> <div class="card__background "> <div class="fit-photo fit-photo "> <picture> <source media="(max-width: 768px)" srcset="https://chanzuckerberg.com/wp-content/uploads/2024/10/24_1024-CZI-The-Power-CZBN-Collaborative-Science-Mobile-BLANK.jpg"> <source media="(min-width: 769px)" srcset="https://chanzuckerberg.com/wp-content/uploads/2024/10/24_1024-CZI-The-Power-CZBN-Collaborative-Science-Desktop-BLANK-opt.jpg"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/10/24_1024-CZI-The-Power-CZBN-Collaborative-Science-Desktop-BLANK-opt.jpg" alt="Priscilla Chan looks into a microscope while a colleague observes her in a well-lit laboratory at the CZ Imaging Institute." object-fit-polyfill /> </picture> </div> </div> <div class="card__container"> <div class="card__surtitle t-category-label background--green color--white">Science & Technology</div> <div class="card__title t-h4--bold color--black" id="card-title-32924">How Biohub Collaboration Unraveled a Medical Mystery</div> <a href="https://chanzuckerberg.com/blog/priscilla-chan-update-2024-biohub-progress/" target="_self" class="button-cta button-cta-- button-cta--light-fill " title="Read Story" aria-label="" > <div class="button-cta__text " >Read Story</div> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </a> </div> </div> </div> <div class="card background-image-card card--align-top card--align-center card--button-bottom list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/blog/priscilla-chan-update-advancing-science-education-ai/" class="card__link" title="Read Story - Our Progress Leveraging AI To Speed Up Biomedical Research" aria-labelledby="card-title-32185"></a> <div class="card__background "> <div class="fit-photo fit-photo "> <picture> <source media="(max-width: 768px)" srcset="https://chanzuckerberg.com/wp-content/uploads/2024/08/24_0731-CZI-Exploring-the-Intersection-of-AI-and-Biology-Mobile-BLANK.jpg"> <source media="(min-width: 769px)" srcset="https://chanzuckerberg.com/wp-content/uploads/2024/08/24_0731-CZI-Exploring-the-Intersection-of-AI-and-Biology-Desktop-BLANK.jpg"> <img src="https://chanzuckerberg.com/wp-content/uploads/2024/08/24_0731-CZI-Exploring-the-Intersection-of-AI-and-Biology-Desktop-BLANK.jpg" alt="Priscilla Chan is speaking into a microphone, smiling, and set against a light blue background." object-fit-polyfill /> </picture> </div> </div> <div class="card__container"> <div class="card__surtitle t-category-label background--green color--white">Science & Technology</div> <div class="card__title t-h4--bold color--black" id="card-title-32185">Our Progress Leveraging AI To Speed Up Biomedical Research</div> <a href="https://chanzuckerberg.com/blog/priscilla-chan-update-advancing-science-education-ai/" target="_self" class="button-cta button-cta-- button-cta--light-fill " title="Read Story" aria-label="" > <div class="button-cta__text " >Read Story</div> <div class="button-cta__arrow cta-arrow cta-arrow--red "> <div class="cta-arrow__bkg"></div> </div> </a> </div> </div> </div> <div class="card background-image-card card--align-top card--align-center card--button-bottom list__row is-active" carousel-item card> <div class="card__wrapper"> <a href="https://chanzuckerberg.com/blog/priscilla-chan-update-ai-biomedical-research/" class="card__link" title="Read Story - 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class="t-h6--semibold">Cherie Butts</span> <span class="members__job t-smallcaps-plain--med">Biogen</span> </div> <div class="members__single"> <div class="members__photo"> <div class="fit-photo fit-photo "> <img src="https://chanzuckerberg.com/wp-content/uploads/2021/04/Advisory-Board_Vivian-Cheung.png" alt="Vivian Cheung, University of Michigan (Advisory Board, CZI Single-Cell Biology)." object-fit-polyfill /> </div> </div> <span class="t-h6--semibold">Vivian Cheung</span> <span class="members__job t-smallcaps-plain--med">University of Michigan</span> </div> <div class="members__single"> <div class="members__photo"> <div class="fit-photo fit-photo "> <img src="https://chanzuckerberg.com/wp-content/uploads/2021/04/Advisory-Board_Keolu-Fox.png" alt="Keolu Fox, University of California, San Diego (Advisory Board, CZI Single-Cell Biology)." object-fit-polyfill /> </div> </div> <span class="t-h6--semibold">Keolu Fox</span> <span class="members__job t-smallcaps-plain--med">University of 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Goodman, New York University (Advisory Board, CZI Single-Cell Biology)." object-fit-polyfill /> </div> </div> <span class="t-h6--semibold">Melody S. Goodman</span> <span class="members__job t-smallcaps-plain--med">New York University </span> </div> <div class="members__single"> <div class="members__photo"> <div class="fit-photo fit-photo "> <img src="https://chanzuckerberg.com/wp-content/uploads/2021/04/Advisory-Board_Latifa-Jackson.png" alt="Latifa Jackson, Howard University (Advisory Board, CZI Single-Cell Biology)." object-fit-polyfill /> </div> </div> <span class="t-h6--semibold">Latifa Jackson</span> <span class="members__job t-smallcaps-plain--med">Howard University</span> </div> <div class="members__single"> <div class="members__photo"> <div class="fit-photo fit-photo "> <img src="https://chanzuckerberg.com/wp-content/uploads/2021/04/Molly-Maleckar.png" alt="Molly Maleckar, Simula Research Laboratory (Advisory Board, CZI Single-Cell Biology)." object-fit-polyfill /> </div> </div> <span class="t-h6--semibold">Molly Maleckar</span> <span class="members__job t-smallcaps-plain--med">Simula Research Laboratory</span> </div> <div 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