Custom Membrane Protein QC Service

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However, the production procedure of membrane proteins is tedious and their structures are intrinsically complicated, which could potentially lead to loss of native activity. Therefore, prior to further application, information regarding basic functional activity is of great essence for proper evaluation of the membrane protein quality and the proceedings and directions of the project. Creative Biolabs offers a comprehensive set of functional analysis services to assist our clients in determining the <em>in vitro</em> characteristics of various membrane proteins. </p> <p class="show-center"> <img loading="lazy" src="static/img/4-1-1-6-custom-membrane-protein-qc-service-1.jpg" width="692" height="227" alt="Membrane Protein (Creative Biolabs Original)" /> </p> <h3> Traditional QC Services </h3> <ul class="ullist"> <li> Enzyme-linked immunosorbent assay (ELISA): Sandwich ELISA represents the most classic method for ligand binding profiling. It is label-free, easy-to-operate, and compatible with a high throughput format. </li> <li> SPR/BLI: Both surface plasmon resonance (SPR) and bio-layer interferometry (BLI) are label-free and capable of measuring real-time quantitative binding affinities and kinetics for membrane proteins interacting with ligands. </li> <li> Tag-lite assay: Tag-lite ligand-binding assay is non-radioactive and widely applicable to a whole range of multi-pass membrane proteins. This strategy is based on the combination of fluorescence detection methods with a covalent labeling technology. Tag-lite also offers adequate freedom and flexibility to build up your assays from target construction to assay development. </li> <li> Radioactive ligand-binding assay (RBA): As the current standard method for the validation of ligand interaction with GPCRs, it is relatively simple but powerful. It can provide sensitive and quantitative information about GPCR affinity for a wide variety of ligands. Three basic types of radioligand-binding experiments are available: saturation, inhibition, and kinetic assays. </li> <li> Fluorescence-based assay: With the wide spectrum of wavelengths, multiple colors can be applied for the detection of a specific target. With more stable probes and more sensitive instruments, we can perform various experiments such as fluorescent intensity measurement, fluorescence polarization, and energy transfer between probes to evaluate ligand binding properties. </li> </ul> <h3> Special QC Services </h3> <ul class="ullist"> <li> GPCRs functional assay -- particularly suited for the investigation of GPCRs function. </li> </ul> <p> There are four subfamilies of G proteins: Gs, Gi/o, Gq/11, and G12/13. The various subfamilies' members use a variety of secondary messengers and signaling routes to carry out their actions. Originally believed to be mainly involved in membrane attachment and complex formation with inactive GDP-bound 𝜶 subunits, the β𝛾 complexes also regulate a number of effector proteins. We offer functional assays for GPCRs, including cAMP, calcium, inositol phosphate accumulation, and so on. </p> <ul class="ullist"> <li> Electrophysiology -- particularly suited for the investigation of transporter and ion channel function. </li> </ul> <p> This label-free, highly sensitive method is generally considered as the 'gold standard' for assessing ion channel functions. It can measure the biophysical and pharmacological properties of voltage-gated and ligand-gated ion channels present in cells, membrane patches, or tissues with unmatched temporal resolution. </p> <p> The information gathered from the initial step of analysis provides a guideline for membrane protein quality assurance and downstream applications. Aided by this versatile platform, scientists of <strong>Creative Biolabs</strong> are glad to tailor specific functional validation service packages to fit your timeline and R&D budget. Please <a class="inquiry-button">contact us</a> for more information and a detailed quote. </p><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. 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