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Syed</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">University of the Punjab, Lahore, Pakistan</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://sakarya.academia.edu/MBilen"><img class="profile-avatar u-positionAbsolute" alt="Mahmut Bilen" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/4244377/1680642/95173809/s200_mahmut.bilen.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://sakarya.academia.edu/MBilen">Mahmut Bilen</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Sakarya University</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://weillcornell.academia.edu/DikomaShungu"><img class="profile-avatar u-positionAbsolute" alt="Dikoma C Shungu" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/10236294/3137818/19401748/s200_dikoma.shungu.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://weillcornell.academia.edu/DikomaShungu">Dikoma C Shungu</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Weill Cornell Medicine</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://djasljsa.academia.edu/AdnanAwad"><img class="profile-avatar u-positionAbsolute" alt="Adnan Awad" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/17255937/5084286/5829848/s200_adnan.awad.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://djasljsa.academia.edu/AdnanAwad">Adnan Awad</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">The University Of Jordan</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://sheffield.academia.edu/LyudmilaSMihaylova"><img class="profile-avatar u-positionAbsolute" alt="Lyudmila S Mihaylova" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/25231054/6866449/31438740/s200_lyudmila_s.mihaylova.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://sheffield.academia.edu/LyudmilaSMihaylova">Lyudmila S Mihaylova</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">The University of Sheffield</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://129nowoursynowskastr.academia.edu/KOracz"><img class="profile-avatar u-positionAbsolute" alt="Krystyna Oracz" border="0" onerror="if (this.src != '//a.academia-assets.com/images/s200_no_pic.png') this.src = '//a.academia-assets.com/images/s200_no_pic.png';" width="200" height="200" src="https://0.academia-photos.com/38281181/10918276/17335819/s200_krystyna.oracz.jpg" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://129nowoursynowskastr.academia.edu/KOracz">Krystyna Oracz</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Warsaw University of Life Sciences</p></div></div><div class="suggested-user-card"><div class="suggested-user-card__avatar social-profile-avatar-container"><a href="https://wlu-ca.academia.edu/FGuinel"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></a></div><div class="suggested-user-card__user-info"><a class="suggested-user-card__user-info__header ds2-5-body-sm-bold ds2-5-body-link" href="https://wlu-ca.academia.edu/FGuinel">F. Guinel</a><p class="suggested-user-card__user-info__subheader ds2-5-body-xs">Wilfrid Laurier University</p></div></div></ul></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="43864210" href="https://www.academia.edu/Documents/in/Sustainable_Developments"><div id="js-react-on-rails-context" style="display:none" data-rails-context="{"inMailer":false,"i18nLocale":"en","i18nDefaultLocale":"en","href":"https://independent.academia.edu/RalphObendorf","location":"/RalphObendorf","scheme":"https","host":"independent.academia.edu","port":null,"pathname":"/RalphObendorf","search":null,"httpAcceptLanguage":null,"serverSide":false}"></div> <div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Sustainable Developments"]}" data-trace="false" data-dom-id="Pill-react-component-7091bdc9-0490-4d85-ab6e-d65558eb9121"></div> <div id="Pill-react-component-7091bdc9-0490-4d85-ab6e-d65558eb9121"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="43864210" href="https://www.academia.edu/Documents/in/Magnetic_Resonance_Spectroscopy"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Magnetic Resonance Spectroscopy"]}" data-trace="false" data-dom-id="Pill-react-component-2160163c-86e4-4096-b54e-055b48ecb55f"></div> <div id="Pill-react-component-2160163c-86e4-4096-b54e-055b48ecb55f"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="43864210" href="https://www.academia.edu/Documents/in/Seeds"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Seeds"]}" data-trace="false" 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data-dom-id="Pill-react-component-d6d1f477-46fb-4ddd-8d04-6de4c23e6172"></div> <div id="Pill-react-component-d6d1f477-46fb-4ddd-8d04-6de4c23e6172"></div> </a></div></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Ralph Obendorf</h3></div><div class="js-work-strip profile--work_container" data-work-id="122074310"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074310/Expression_of_the_maize_GALACTINOL_SYNTHASE_gene_family_I_Expression_of_two_different_genes_during_seed_development_and_germination"><img alt="Research paper thumbnail of Expression of the maize GALACTINOL SYNTHASE gene family: (I) Expression of two different genes during seed development and germination" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074310/Expression_of_the_maize_GALACTINOL_SYNTHASE_gene_family_I_Expression_of_two_different_genes_during_seed_development_and_germination">Expression of the maize GALACTINOL SYNTHASE gene family: (I) Expression of two different genes during seed development and germination</a></div><div class="wp-workCard_item"><span>Physiologia Plantarum</span><span>, Aug 1, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT GALACTINOL SYNTHASE (GOLS) acts on UDP-galactose and myo-inositol to form galactinol. Th...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT GALACTINOL SYNTHASE (GOLS) acts on UDP-galactose and myo-inositol to form galactinol. The production of galactinol is thought to be the committed step in the biosynthesis of the raffinose family oligosaccharides (RFOs), a group of carbohydrates hypothesized to play a number of roles in plant development. Three cDNAs for maize (Zea mays L. inbred line B73) GOLS (ZmGOLS) were identified from the Pioneer Hi-Bred expressed sequence database. The recombinant proteins from these cDNAs synthesize galactinol. Transcript amounts in developing and germinating seeds were determined using northern blot, ribonuclease protection assays, and multiplex RT-PCR. ZmGOLS1 mRNA was not detected in any of the tissues examined. ZmGOLS3 transcripts accumulated late during seed development and were present in mature seeds but declined quickly to undetectable amounts following imbibition. Experiments in which germination was interrupted by desiccation, but not by cold, heat, or salt stress, detected ZmGOLS2 transcripts. Soluble sugar amounts and GALACTINOL SYNTHASE activity were determined for developing maize seeds as well as for mature seeds during germination and upon stress during germination. Neither enzyme activity nor galactinol or raffinose quantities could be related to ZmGOLS transcript abundance. Galactinol amounts were most closely related to myo-inositol quantities during development and early germination. Maturation desiccation appears to be a switch delineating a realm of expression for ZmGOLS3, predominant during seed development, from that of ZmGOLS2, principally associated with post-imbibition dehydration stress.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074310"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074310"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074310; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074309"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074309/Galactosyl_Cyclitols_and_Raffinose_Family_Oligosaccharides_in_Relation_to_Desiccation_Tolerance_of_Pea_and_Soyabean_Seedlings"><img alt="Research paper thumbnail of Galactosyl Cyclitols and Raffinose Family Oligosaccharides in Relation to Desiccation Tolerance of Pea and Soyabean Seedlings" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074309/Galactosyl_Cyclitols_and_Raffinose_Family_Oligosaccharides_in_Relation_to_Desiccation_Tolerance_of_Pea_and_Soyabean_Seedlings">Galactosyl Cyclitols and Raffinose Family Oligosaccharides in Relation to Desiccation Tolerance of Pea and Soyabean Seedlings</a></div><div class="wp-workCard_item"><span>Current plant science and biotechnology in agriculture</span><span>, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The loss of desiccation tolerance of pea and soyabean seedlings and associated changes in their s...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The loss of desiccation tolerance of pea and soyabean seedlings and associated changes in their soluble carbohydrates were studied. For the desiccation tolerance test, intact seedlings were dried rapidly at 12% relative humidity, or slowly in a series of decreasing relative humidities, followed by rehydration. Soluble carbohydrates were assayed before and after drying. Survival and regrowth of shoot, hypocotyl and root tissues were recorded. Soyabean hypocotyl and root tissues los t desiccation tolerance within 36 h during germination in association with the loss of raffinose, stachyose, galactopinitol A, galactopinitol B, ciceritol, and fagopyritol B1. In contrast, fructose, glucose and sorbitol accumulated. Sucrose levels increased during desiccation, but there was no effect of drying on changes of other soluble carbohydrates. Pea root tissues lost desiccation tolerance during the first 36 h of germination. Unlike roots, 80% of pea epicotyls survived the slow drying treatment and 40% survived the fast drying treatment of seedlings at up to 96 h after imbibition. Sucrose levels increased 5 to 10 fold in root and hypocotyl tissues and even more in epicotyls during desiccation. Glucose and fructose increased during germination and remained elevated after drying. The data indicate that monosaccharides may induce desiccation damage whereas oligosaccharides are not a prerequisite for desiccation tolerance of pea seedling epicotyls.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074309"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074309"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074309; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074309]").text(description); $(".js-view-count[data-work-id=122074309]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074309; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074309']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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</script> <div class="js-work-strip profile--work_container" data-work-id="122074308"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074308/Damage_and_tolerance_in_retrospect_and_prospect"><img alt="Research paper thumbnail of Damage and tolerance in retrospect and prospect" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074308/Damage_and_tolerance_in_retrospect_and_prospect">Damage and tolerance in retrospect and prospect</a></div><div class="wp-workCard_item"><span>CABI Publishing eBooks</span><span>, 2002</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074308"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074308"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074308; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074308]").text(description); $(".js-view-count[data-work-id=122074308]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074308; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074308']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074308]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074308,"title":"Damage and tolerance in retrospect and prospect","internal_url":"https://www.academia.edu/122074308/Damage_and_tolerance_in_retrospect_and_prospect","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074306"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074306/Recent_accomplishments_and_new_opportunities_in_seed_research"><img alt="Research paper thumbnail of Recent accomplishments and new opportunities in seed research" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074306/Recent_accomplishments_and_new_opportunities_in_seed_research">Recent accomplishments and new opportunities in seed research</a></div><div class="wp-workCard_item"><span>CABI Publishing eBooks</span><span>, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This chapter provides a brief introduction on the different breakthroughs regarding studies relat...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This chapter provides a brief introduction on the different breakthroughs regarding studies relating to seed research in Salamanca, Spain. These include: seed development; seed germination and dormancy; desiccation and other stress tolerance and conservation; seed ecology; seed banks and preservation of germplasm; and seed biotechnology. Also included are new opportunities for studies on seed biology.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074306"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074306"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074306; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074306]").text(description); $(".js-view-count[data-work-id=122074306]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074306; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074306']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074306]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074306,"title":"Recent accomplishments and new opportunities in seed research","internal_url":"https://www.academia.edu/122074306/Recent_accomplishments_and_new_opportunities_in_seed_research","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074305"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074305/Imbibitional_surface_damage_in_isolated_hypocotyl_root_axes_of_soybean_Glycine_max_L_Merr_cv_Chippewa_64_"><img alt="Research paper thumbnail of Imbibitional surface damage in isolated hypocotyl-root axes of soybean (Glycine max (L.) Merr. cv. Chippewa 64)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074305/Imbibitional_surface_damage_in_isolated_hypocotyl_root_axes_of_soybean_Glycine_max_L_Merr_cv_Chippewa_64_">Imbibitional surface damage in isolated hypocotyl-root axes of soybean (Glycine max (L.) Merr. cv. Chippewa 64)</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074305"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074305"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074305; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074305]").text(description); $(".js-view-count[data-work-id=122074305]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074305; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074305']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074305]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074305,"title":"Imbibitional surface damage in isolated hypocotyl-root axes of soybean (Glycine max (L.) Merr. cv. Chippewa 64)","internal_url":"https://www.academia.edu/122074305/Imbibitional_surface_damage_in_isolated_hypocotyl_root_axes_of_soybean_Glycine_max_L_Merr_cv_Chippewa_64_","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074304"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074304/Buckwheat_seed_set_in_planta_and_during_in_vitro_inflorescence_culture_evaluation_of_temperature_and_water_deficit_stress"><img alt="Research paper thumbnail of Buckwheat seed set in planta and during in vitro inflorescence culture: evaluation of temperature and water deficit stress" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074304/Buckwheat_seed_set_in_planta_and_during_in_vitro_inflorescence_culture_evaluation_of_temperature_and_water_deficit_stress">Buckwheat seed set in planta and during in vitro inflorescence culture: evaluation of temperature and water deficit stress</a></div><div class="wp-workCard_item"><span>Seed Science Research</span><span>, Apr 25, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT Common buckwheat (Fagopyrum esculentum Moench) plants produce many flowers, but fewer se...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT Common buckwheat (Fagopyrum esculentum Moench) plants produce many flowers, but fewer seeds. Seed set is highly variable among years, between plants, and during the period of flowering within a plant or raceme. Seasonal variations suggest that temperature and water-deficit stresses are important factors for seed set. The effects of mild temperature and water-deficit stresses on seed set and seed filling were determined in planta and in vitro. An in vitro method to culture matched sets of racemes from individual plants was used for precise comparisons between experimental treatments. Buckwheat racemes form new flowers continuously during several weeks in planta. Seed set resulting in yield occurs during the first 2–3 weeks of flowering and then rapidly declines in planta independently of mild stresses. Plants grown at 18°C have 40% increased seed set, set seeds over a longer duration, and produce 40% more dry matter per seed than plants grown at 25°C. Similar patterns occurred in vitro. A 3-day water-deficit stress during the first week of flowering reduced the number of seeds by 50%without a reduction in seed size and dry weight, or the number of flowers formed in planta or in vitro. The effect of water-deficit stress continued after rewatering and subsequently was expressed as a reduction in fertility in newly formed flowers, both in planta and in vitro. Mild temperature and water-deficit stresses affected both female and male components of seed set in common buckwheat, resulting in a persistent but non-additive reduction in sink strength.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074304"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074304"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074304; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074304]").text(description); $(".js-view-count[data-work-id=122074304]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074304; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074304']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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</script> <div class="js-work-strip profile--work_container" data-work-id="122074303"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074303/Free_cyclitol_unloading_from_seed_coats_on_stem_leaf_pod_explants_of_low_raffinose_low_stachyose_low_phytin_soybean"><img alt="Research paper thumbnail of Free cyclitol unloading from seed coats on stem–leaf–pod explants of low-raffinose, low-stachyose, low-phytin soybean" class="work-thumbnail" src="https://attachments.academia-assets.com/116809875/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074303/Free_cyclitol_unloading_from_seed_coats_on_stem_leaf_pod_explants_of_low_raffinose_low_stachyose_low_phytin_soybean">Free cyclitol unloading from seed coats on stem–leaf–pod explants of low-raffinose, low-stachyose, low-phytin soybean</a></div><div class="wp-workCard_item"><span>Seed Science Research</span><span>, Sep 24, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Raffinose, stachyose and phytin are undesirable compounds for soybean food and animal feed produc...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Raffinose, stachyose and phytin are undesirable compounds for soybean food and animal feed products. In seeds, raffinose and stachyose are believed to contribute to desiccation and cold stress tolerance. Thus, removal of these compounds from soybean by genetic mutation has resulted in a more commercially desirable composition, but potentially less physiologically viable seeds. In an effort to develop a method to improve viability and seed storability in soybean, stem-leaf-pod explants of three low raffinose, low stachyose lines, two of which were also low in phytin, and a check line were fed solutions containing D-chiro-inositol, myo-inositol or D-pinitol, free cyclitols which unload through the seed coat to the developing embryo where they accumulate as fagopyritols, galactinol and galactopinitols, respectively, during seed maturation. Increased galactopinitol and fagopyritol accumulation may substitute for the roles of raffinose and stachyose in low raffinose, stachyose and phytin seeds. Explants of all lines unloaded D-chiro-inositol, myo-inositol and D-pinitol. Fed D-chiro-inositol accumulated in leaf tissues demonstrating uptake into explants. Fed D-chiro-inositol and myo-inositol accumulated in pod wall and seed coat tissues of one or more lines. The results indicate that D-chiro-inositol was unloaded from the seed coat to the embryo in increased amounts after feeding. The potential use of increased maternal D-chiro-inositol for synthesis of fagopyritols in embryos to improve seed performance in low-stachyose and low-phytin soybean seeds is supported. The seed coat cup unloading of fed free cyclitols may provide a model system to test effective unloading of upregulated maternally synthesized cyclitols.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8759712d9adbea034df2de6ebe932f4e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809875,"asset_id":122074303,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809875/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074303"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074303"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074303; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074302"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074302/Soybean_Seed_Water_Relations_during_i_in_Situ_i_and_i_in_Vitro_i_Growth_and_Maturation"><img alt="Research paper thumbnail of Soybean Seed Water Relations during <i>in Situ</i> and <i>in Vitro</i> Growth and Maturation" class="work-thumbnail" src="https://attachments.academia-assets.com/116809847/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074302/Soybean_Seed_Water_Relations_during_i_in_Situ_i_and_i_in_Vitro_i_Growth_and_Maturation">Soybean Seed Water Relations during <i>in Situ</i> and <i>in Vitro</i> Growth and Maturation</a></div><div class="wp-workCard_item"><span>Plant Physiology</span><span>, Feb 1, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Water, osmotic, and pressure potentials of soybean (Glycine max [L.] Merrill) embryos and related...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Water, osmotic, and pressure potentials of soybean (Glycine max [L.] Merrill) embryos and related matemal tissues were measured during periods of seed growth and maturation to test the involvement of embryo water relations in seed maturation. Seeds were matured in situ or in an in vitro liquid culture medium in detached pods or as isolated seeds. Changes in water relations of embryo tissues were independent of maternal tissues. During seed maturation in situ, water and osmotic potentials in both embryo and maternal tissues declined sharply near the time of maximum dry weight. During in vitro seed culture with and without pods, water and osmotic potentials in axis and cotyledon tissues declined continuously during growth. Water and osmotic potentials of the seed coat, which was present only during in vitro seed culture with pods, changed little during the culture period. Positive turgor in the embryo was maintained beyond maximum dry weight and the loss of green color during in vitro culture but declined to zero at maturity in situ. The osmotic potential in embryo tissues declined from-1.1 megapascals at early pod fill to between-1.65 and-2.2 megapascals at maximum seed dry weight across all maturation environments. It is suggested that the decreasing osmotic potential in the growing soybean embryo reaches a threshold level that is associated with cessation of growth and onset of seed maturation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f9a74fb54553b8ef96ac44b1b883800f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809847,"asset_id":122074302,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809847/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074302"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074302"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074302; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074301"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074301/Polyribosome_formation_and_mRNA_synthesis_in_soybean_embryonic_axes_during_early_germination"><img alt="Research paper thumbnail of Polyribosome formation and mRNA synthesis in soybean embryonic axes during early germination" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074301/Polyribosome_formation_and_mRNA_synthesis_in_soybean_embryonic_axes_during_early_germination">Polyribosome formation and mRNA synthesis in soybean embryonic axes during early germination</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074301"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074301"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074301; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074301]").text(description); $(".js-view-count[data-work-id=122074301]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074301; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074301']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074301]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074301,"title":"Polyribosome formation and mRNA synthesis in soybean embryonic axes during early germination","internal_url":"https://www.academia.edu/122074301/Polyribosome_formation_and_mRNA_synthesis_in_soybean_embryonic_axes_during_early_germination","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074300"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074300/Relationship_of_Stelar_Lesions_to_Radicle_Growth_in_Corn_Seedlings_1"><img alt="Research paper thumbnail of Relationship of Stelar Lesions to Radicle Growth in Corn Seedlings 1" class="work-thumbnail" src="https://attachments.academia-assets.com/116923618/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074300/Relationship_of_Stelar_Lesions_to_Radicle_Growth_in_Corn_Seedlings_1">Relationship of Stelar Lesions to Radicle Growth in Corn Seedlings 1</a></div><div class="wp-workCard_item"><span>Agronomy Journal</span><span>, Nov 1, 1979</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Chilling during imbibition of Zcu mays L. kernels resulted in anatomical lesions acros the stele ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Chilling during imbibition of Zcu mays L. kernels resulted in anatomical lesions acros the stele of radicles, and subsequently in reduced growth of radicles. The objective was to determine if stelar lesion formation accounted for the radicle growth reduction. Inheritance patterns, 'source of seedstodrs, maturity of kernels, and surgical treatments of pericarp and endosperm were used to compare lesion occurrence and radicle growth in l a b ratory evaluations of field-grown seedstocks. Chilling injury of 5% (low) moisture kernels was measured as radicle growth reduction after a 48 hour hydration treatment at 5 C followed by 4 days at 25 C when compared to Similarly treated controls with 13% moisture kernels.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="72ef01276a3d55b1a7b02e9d8bf45e76" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116923618,"asset_id":122074300,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116923618/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074300"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074300"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074300; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074299"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074299/Purification_and_molecular_structure_of_digalactosyl_myo_inositol_DGMI_trigalactosyl_myo_inositol_TGMI_and_fagopyritol_B3_from_common_buckwheat_seeds_by_NMR"><img alt="Research paper thumbnail of Purification and molecular structure of digalactosyl myo-inositol (DGMI), trigalactosyl myo-inositol (TGMI), and fagopyritol B3 from common buckwheat seeds by NMR" class="work-thumbnail" src="https://attachments.academia-assets.com/116809876/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074299/Purification_and_molecular_structure_of_digalactosyl_myo_inositol_DGMI_trigalactosyl_myo_inositol_TGMI_and_fagopyritol_B3_from_common_buckwheat_seeds_by_NMR">Purification and molecular structure of digalactosyl myo-inositol (DGMI), trigalactosyl myo-inositol (TGMI), and fagopyritol B3 from common buckwheat seeds by NMR</a></div><div class="wp-workCard_item"><span>Carbohydrate Research</span><span>, Oct 1, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Three galactosyl cyclitols, digalactosyl myo-inositol (assigned the trivial name DGMI), trigalact...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Three galactosyl cyclitols, digalactosyl myo-inositol (assigned the trivial name DGMI), trigalactosyl myoinositol (assigned the trivial name TGMI), and trigalactosyl D-chiro-inositol (fagopyritol B3), were isolated from common buckwheat (Fagopyrum esculentum Moench) seeds. Structures of the three compounds were determined by 2D NMR spectroscopy. DGMI is aD -galactopyranosyl-(1?6)-aD -galactopyranosyl-(1?1)-1L-myo-inositol, TGMI is aD -galactopyranosyl-(1?6)-aD -galactopyranosyl-(1?6)-aD -galactopyranosyl-(1?1)-1L-myo-inositol, and fagopyritol B3 is aD -galactopyranosyl-(1?6)-aD -galactopyranosyl-(1? 6)-aD -galactopyranosyl-(1?2)-1D-chiro-inositol. DGMI and TGMI are higher oligomers of galactinol, a major galactosyl donor in plant seeds. Fagopyritol B3 is a higher oligomer of the fagopyritol B series and one of six fagopyritols found in buckwheat seeds and in the bran milling fraction. Samples of TGMI and fagopyritol B3 also contained the compounds N-(b-glucopyranosyl)-nicotinic acid and b-D-apiofuranosyl-(1?6)-b-D-(1-O-methyl)-glucopyranoside.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5014c340c177d8b61086d0acbd49b692" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809876,"asset_id":122074299,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809876/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074299"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074299"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074299; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074298"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074298/Soybean_seed_pectinesterase"><img alt="Research paper thumbnail of Soybean seed pectinesterase" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074298/Soybean_seed_pectinesterase">Soybean seed pectinesterase</a></div><div class="wp-workCard_item"><span>Seed Science Research</span><span>, Dec 1, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Methanol accumulates in axis tissues of maturing soybean seeds, correlating with preharvest seed ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Methanol accumulates in axis tissues of maturing soybean seeds, correlating with preharvest seed deterioration. Accumulation of methanol appears to be associated with the enzymic demethylation of pectin methyl esters by pectinesterase (PE; EC 3.1.1.11). To characterize PE in developing and maturing soybean (Glycine max (L.) Merrill) seeds, enzyme activity was assayed in axis and cotyledon tissues. Activity per g fresh weight was 20–25 times higher in axes than in cotyledons with highest activities between 45 and 60 days after flowering (DAF). Twenty to 33% of the total PE activity was in the ‘soluble’ form (extracted with water, 0.5 M sucrose, 1 M sucrose and water). Soluble and cell-wall-bound PE (subsequently extracted with 1 M NaCI) were purified and characterized from axes of seeds at 45–60 DAF. Purification of PE was achieved through concentration of extracts by ultra-filtration, precipitation with ammonium sulfate (30–80% saturation), dialysis, gel filtration on Sephadex G-75 columns, and ion exchange chromatography on CM Sepharose CL-6B. Further purification of both soluble and bound PE was by isoelectric focusing (IEF) on ultrathin layers of polyacrylamide gel with simultaneous detection of protein and PE activity. It was possible to follow seven bands exhibiting PE activity with pl values between 6.0 and 9.5 in 1 M NaCI-extracts of total homogenates. Differences in the IEF patterns of bound and soluble PE were observed. Whereas the bound enzyme exhibited more basic PE bands (pl 8–9.5), the soluble enzyme had more active bands at pl 6.5, 7.0 and 7.5. The Mr was close to 33 000 and the pH optimum was 7.8 for both soluble and bound PE.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074298"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074298"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074298; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074298]").text(description); $(".js-view-count[data-work-id=122074298]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074298; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074298']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074298]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074298,"title":"Soybean seed pectinesterase","internal_url":"https://www.academia.edu/122074298/Soybean_seed_pectinesterase","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074297"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074297/Soluble_sugars_and_flatulence_producing_oligosaccharides_in_maturing_yellow_lupin_i_Lupinus_luteus_i_L_seeds"><img alt="Research paper thumbnail of Soluble sugars and flatulence-producing oligosaccharides in maturing yellow lupin (<i>Lupinus luteus</i>L.) seeds" class="work-thumbnail" src="https://attachments.academia-assets.com/116809873/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074297/Soluble_sugars_and_flatulence_producing_oligosaccharides_in_maturing_yellow_lupin_i_Lupinus_luteus_i_L_seeds">Soluble sugars and flatulence-producing oligosaccharides in maturing yellow lupin (<i>Lupinus luteus</i>L.) seeds</a></div><div class="wp-workCard_item"><span>Seed Science Research</span><span>, Jun 1, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The flatulence-producing soluble oligosaccharides are an important component of lupin seeds and w...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The flatulence-producing soluble oligosaccharides are an important component of lupin seeds and were assayed to establish the pattern of their accumulation in relation to germinability during seed development and maturation. Maturing yellow lupin cv. Juno seeds were harvested at 5-day intervals from 15 to 45 days after flowering (DAF). Seed fresh mass increased to a maximum at 35 DAF followed by a decrease when axis and cotyledon tissues changed colour from green to yellow. Maximum seed fresh mass corresponded to the maximum seed size. Seed dry mass continuously increased until 40 DAF. About 75% of mature seed dry mass was in cotyledons, 22% in testa, and 3% in axis. Maximum seed germinability occurred at 45 DAF after maximum seed dry mass and desiccation. Maturing yellow lupin seeds were desiccation tolerant. Mature dry seeds contained 10.9% oligosaccharides and 1.5% sucrose. During seed growth stachyose accumulation preceded accumulation of raffinose and verbascose. The highest rate of ohgosaccharide accumulation appeared during seed desiccation and correlated with the acquisition of the ability to germinate. The sucrose :oligosaccharide ratio continuously decreased reaching 0.13 in mature seeds. Accumulation of oligosaccharides in maturing yellow lupin seeds is associated with seed germinability and seed desiccation tolerance.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d1b27e0af8737ddd6e78c306988321ff" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809873,"asset_id":122074297,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809873/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074297"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074297"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074297; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074296"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074296/Purification_and_molecular_structure_of_two_digalactosyl_d_chiro_inositols_and_two_trigalactosyl_d_chiro_inositols_from_buckwheat_seeds"><img alt="Research paper thumbnail of Purification and molecular structure of two digalactosyl d-chiro-inositols and two trigalactosyl d-chiro-inositols from buckwheat seeds" class="work-thumbnail" src="https://attachments.academia-assets.com/116809878/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074296/Purification_and_molecular_structure_of_two_digalactosyl_d_chiro_inositols_and_two_trigalactosyl_d_chiro_inositols_from_buckwheat_seeds">Purification and molecular structure of two digalactosyl d-chiro-inositols and two trigalactosyl d-chiro-inositols from buckwheat seeds</a></div><div class="wp-workCard_item"><span>Carbohydrate Research</span><span>, Mar 1, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Two digalactosyl D-chiro-inositols and two trigalactosyl D-chiro-inositols, members of the fagopy...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Two digalactosyl D-chiro-inositols and two trigalactosyl D-chiro-inositols, members of the fagopyritol A series and fagopyritol B series, were isolated from buckwheat (Fagopyrum esculentum Moench) seeds. Structures of the first three were determined by 1 H and 13 C NMR. Fagopyritol B2 is aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 2)-1D-chiro-inositol, and fagopyritol A2 is aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 3)-1D-chiroinositol. Fagopyritol A3, a trigalactosyl D-chiro-inositol, is aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 3)-1D-chiro-inositol. From analysis of hydrolysis products, the second trigalactosyl D-chiro-inositol, fagopyritol B3, is aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 2)-1D-chiro-inositol.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="094b88997d774882ebef9706c20d1f3e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809878,"asset_id":122074296,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809878/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074296"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074296"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074296; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074295"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074295/Quantitative_Assessment_of_Some_Factors_Limiting_Seed_Set_in_Buckwheat"><img alt="Research paper thumbnail of Quantitative Assessment of Some Factors Limiting Seed Set in Buckwheat" class="work-thumbnail" src="https://attachments.academia-assets.com/116809872/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074295/Quantitative_Assessment_of_Some_Factors_Limiting_Seed_Set_in_Buckwheat">Quantitative Assessment of Some Factors Limiting Seed Set in Buckwheat</a></div><div class="wp-workCard_item"><span>Crop Science</span><span>, Nov 1, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">estimate of fitness, for example, seed set. Likewise, the theory of reproductive strategies of pl...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">estimate of fitness, for example, seed set. Likewise, the theory of reproductive strategies of plants has been in-A healthy buckwheat (Fagopyrum esculentum Moench) plant profluenced by our ability to determine the mechanisms by duces numerous flowers but few seeds. The objective of this study was to investigate mechanisms responsible for the low frequency of which selection may occur. seed set. Newly open and receptive flowers were pollinated at weekly Reduced seed number can be caused by: (i) insuffiintervals from 4 to 12 wk after seeding (WAS) on selected racemes cient pollination, (ii) sexual selection of male and female of greenhouse-grown plants. Flower number, seed set, abortion, and gametophytes, and (iii) resource limitation of the materanatomical observations of egg sacs and proembryos were analyzed. nal plant (Bawa and Webb, 1984). The male component Although flowers formed continuously for 7 wk, the period of maxiof fitness has been assessed in Campsis radicans (Bertin, mum seed set under controlled conditions was brief (1-2 wk). Compe-1982), Calathea ovandensis (Horvitz and Schemske, 1988), tition within the same nutritional unit (raceme) did not account for Cassia fasiculata (Lee and Bazzaz, 1982), Agave mckellow average seed set per raceme. Neither the number of flowers nor veyana (Sutherland and Delph, 1984), and Lithopserfrequency of seed set was decreased when developing seeds were on mum caroliniense (Weller, 1980). In some cases the evithe same raceme. Abortion (≈10%) was not a major factor limiting seed set. Eighty percent of the flowers failed to initiate seeds even dence indicates that excess flowers contribute to male under controlled growing conditions when hand pollinated. Approxi-fitness by acting as pollen donors (Agave), while in mately 20% of megagametophytes appeared to be defective at antheother cases (Cassia, Lithospermum, Calathea, Campsis) sis. The proportion of ovules containing a proembryo at 24 h after it does not. Sutherland (1987) pooled published obserpollination (HAP) paralleled the proportion initiating seeds, indicatvations of 445 species to test hypotheses related to male ing that fertilization is a limiting process. Pollen tube remnants were fitness. He concluded that excess flowers that fail to observed near the micropyle in 65% of the unfertilized ovules at 24 form mature fruits do not contribute to female fitness HAP. Lack of fertilization accounts for most of the reduction in seed (fruit or seed set) because they abort regardless of their set, appears to be caused by failure of the pollen tube to grow into pollination history. They may act as pollen donors, howthe micropyle, and increases with plant age at pollination. Flowers ever, and thus contribute to male fitness. Conditions for that form late in the plant cycle contribute little to seed set and may be ignored in yield management decisions.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="86ae08eb3149a87fb3b554358c05b16d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809872,"asset_id":122074295,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809872/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074295"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074295"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074295; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074294"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074294/Raffinose_accumulation_in_maize_embryos_in_the_absence_of_a_fully_functional_Vp1_gene_product"><img alt="Research paper thumbnail of Raffinose accumulation in maize embryos in the absence of a fully functional Vp1 gene product" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074294/Raffinose_accumulation_in_maize_embryos_in_the_absence_of_a_fully_functional_Vp1_gene_product">Raffinose accumulation in maize embryos in the absence of a fully functional Vp1 gene product</a></div><div class="wp-workCard_item"><span>Planta</span><span>, Sep 26, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">... type kernels segre-gated on the same ears (Vp1-1695, purple) also were desiccation tolerant i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... type kernels segre-gated on the same ears (Vp1-1695, purple) also were desiccation tolerant in association with a sucrose:raffi-nose mass ... vp1-McWhirter, vp1-A1, and vp1-C821708 were produced at Homestead, Fla., USA during winter 1993/1994 and at Aurora, NY, USA in ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074294"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074294"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074294; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074294]").text(description); $(".js-view-count[data-work-id=122074294]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074294; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074294']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074294]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074294,"title":"Raffinose accumulation in maize embryos in the absence of a fully functional Vp1 gene product","internal_url":"https://www.academia.edu/122074294/Raffinose_accumulation_in_maize_embryos_in_the_absence_of_a_fully_functional_Vp1_gene_product","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074293"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074293/Influence_of_Age_and_Illumination_on_Distribution_of_Several_Calvin_Cycle_Enzymes_in_Greening_Barley_Leaves"><img alt="Research paper thumbnail of Influence of Age and Illumination on Distribution of Several Calvin Cycle Enzymes in Greening Barley Leaves" class="work-thumbnail" src="https://attachments.academia-assets.com/116809871/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074293/Influence_of_Age_and_Illumination_on_Distribution_of_Several_Calvin_Cycle_Enzymes_in_Greening_Barley_Leaves">Influence of Age and Illumination on Distribution of Several Calvin Cycle Enzymes in Greening Barley Leaves</a></div><div class="wp-workCard_item"><span>Plant Physiology</span><span>, May 1, 1970</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Activities of phosphoriboisomerase, phosphoribulokinase, and ribulose 1,5-diphosphate carboxylase...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Activities of phosphoriboisomerase, phosphoribulokinase, and ribulose 1,5-diphosphate carboxylase, protein content, and chlorophyll accumulation in dark-grown barley seedlings were measured before and after illumination. Enzymatic activities, levels of soluble protein, and accumulation (upon illumination) of chlorophyll in leaves declined from tips toward the base. In response to increasing time of illumination, chlorophyll accumulation and activities of phosphoribulokinase and ribulose 1 ,5-diphosphate carboxylase (enzymes located in chloroplasts) increased most in tip portions whereas activity of phosphoriboisomerase and levels of soluble protein (constituents not confined to chloroplasts) increased similarly in all sections of the leaf. Maximum activity of phosphoribulokinase and maximum accumulation of chlorophyll shifted toward median portions of the leaf blade with increased age of seedling before illumination. Maximum activity of ribulose 1 ,5-diphosphate carboxylase and maximum level of soluble protein occurred in all leaf sections when the seedlings were 7 days of age before illumination.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="396db83af3286d4989364dcb6b86a299" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809871,"asset_id":122074293,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809871/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074293"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074293"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074293; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074292"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074292/Soluble_carbohydrates_in_mature_soybean_seed_after_feeding_d_chiro_inositol_myo_inositol_or_d_pinitol_to_stem_leaf_pod_explants_of_low_raffinose_low_stachyose_lines"><img alt="Research paper thumbnail of Soluble carbohydrates in mature soybean seed after feeding d-chiro-inositol, myo-inositol, or d-pinitol to stem-leaf-pod explants of low-raffinose, low-stachyose lines" class="work-thumbnail" src="https://attachments.academia-assets.com/116809874/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074292/Soluble_carbohydrates_in_mature_soybean_seed_after_feeding_d_chiro_inositol_myo_inositol_or_d_pinitol_to_stem_leaf_pod_explants_of_low_raffinose_low_stachyose_lines">Soluble carbohydrates in mature soybean seed after feeding d-chiro-inositol, myo-inositol, or d-pinitol to stem-leaf-pod explants of low-raffinose, low-stachyose lines</a></div><div class="wp-workCard_item"><span>Plant Science</span><span>, Nov 1, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="03689b79b703f5ab9a35e534583abfe8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809874,"asset_id":122074292,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809874/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074292"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074292"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074292; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074226"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074226/Effect_of_Seed_Moisture_on_Temperature_Sensitivity_During_Imbibition_of_Soybean_sup_1_sup_"><img alt="Research paper thumbnail of Effect of Seed Moisture on Temperature Sensitivity During Imbibition of Soybean <sup>1</sup>" class="work-thumbnail" src="https://attachments.academia-assets.com/116809802/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074226/Effect_of_Seed_Moisture_on_Temperature_Sensitivity_During_Imbibition_of_Soybean_sup_1_sup_">Effect of Seed Moisture on Temperature Sensitivity During Imbibition of Soybean <sup>1</sup></a></div><div class="wp-workCard_item"><span>Crop Science</span><span>, Sep 1, 1970</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Seeds of 12 soybean {Glycine max (L.) Merr.} varieties were equilibrated to 6% or 16% moisture be...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Seeds of 12 soybean {Glycine max (L.) Merr.} varieties were equilibrated to 6% or 16% moisture before imbibition at cold (5 C) temperature, warm (25 C) temperature, or warm followed by exposure to cold. After imbibition, seeds were transferred to prewetted autoclaved soil mix in a greenhouse for 2 weeks. The number of seedlings surviving, height to apical bud, and dry weight of aerial parts without cotyledons were determined. Imbibition of low moisture seeds at 5 C caused reduction in survival, dry matter accumulation, and height of seedlings, but no reduction was observed with high moisture seeds. When low moisture seeds were imbibed at warm temperature before exposure to cold, no reduction was noted. The effect of initial seed moisture on sensitivity to chilling during imbibition was reversible. While some varieties were tolerant to cold imbibition of low moisture seeds, other varieties had up to 75% reduction in survival, 38% reduction in dry matter accumulation per seedling, and 50% reduction in plant height when compared to warm imbibition. Imbibition treatments which resulted in greatest seedling injury also decreased the capacity of seedlings to utilize cotyledonary reserves. Our results suggest that adjusting seed moisture to 16% before seeding could improve seedling establishment of soybean in cold soil.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="33bd126ecc084d1a679ddc49f6b9d3fd" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809802,"asset_id":122074226,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809802/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074226"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074226"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074226; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="117551496"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/117551496/Cyanide_insensitive_respiration_during_soybean_seed_formation_and_germination"><img alt="Research paper thumbnail of Cyanide-insensitive respiration during soybean seed formation and germination" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/117551496/Cyanide_insensitive_respiration_during_soybean_seed_formation_and_germination">Cyanide-insensitive respiration during soybean seed formation and germination</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="117551496"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="117551496"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 117551496; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=117551496]").text(description); $(".js-view-count[data-work-id=117551496]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 117551496; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='117551496']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=117551496]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":117551496,"title":"Cyanide-insensitive respiration during soybean seed formation and germination","internal_url":"https://www.academia.edu/117551496/Cyanide_insensitive_respiration_during_soybean_seed_formation_and_germination","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="4678927" id="papers"><div class="js-work-strip profile--work_container" data-work-id="122074310"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074310/Expression_of_the_maize_GALACTINOL_SYNTHASE_gene_family_I_Expression_of_two_different_genes_during_seed_development_and_germination"><img alt="Research paper thumbnail of Expression of the maize GALACTINOL SYNTHASE gene family: (I) Expression of two different genes during seed development and germination" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074310/Expression_of_the_maize_GALACTINOL_SYNTHASE_gene_family_I_Expression_of_two_different_genes_during_seed_development_and_germination">Expression of the maize GALACTINOL SYNTHASE gene family: (I) Expression of two different genes during seed development and germination</a></div><div class="wp-workCard_item"><span>Physiologia Plantarum</span><span>, Aug 1, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT GALACTINOL SYNTHASE (GOLS) acts on UDP-galactose and myo-inositol to form galactinol. Th...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT GALACTINOL SYNTHASE (GOLS) acts on UDP-galactose and myo-inositol to form galactinol. The production of galactinol is thought to be the committed step in the biosynthesis of the raffinose family oligosaccharides (RFOs), a group of carbohydrates hypothesized to play a number of roles in plant development. Three cDNAs for maize (Zea mays L. inbred line B73) GOLS (ZmGOLS) were identified from the Pioneer Hi-Bred expressed sequence database. The recombinant proteins from these cDNAs synthesize galactinol. Transcript amounts in developing and germinating seeds were determined using northern blot, ribonuclease protection assays, and multiplex RT-PCR. ZmGOLS1 mRNA was not detected in any of the tissues examined. ZmGOLS3 transcripts accumulated late during seed development and were present in mature seeds but declined quickly to undetectable amounts following imbibition. Experiments in which germination was interrupted by desiccation, but not by cold, heat, or salt stress, detected ZmGOLS2 transcripts. Soluble sugar amounts and GALACTINOL SYNTHASE activity were determined for developing maize seeds as well as for mature seeds during germination and upon stress during germination. Neither enzyme activity nor galactinol or raffinose quantities could be related to ZmGOLS transcript abundance. Galactinol amounts were most closely related to myo-inositol quantities during development and early germination. Maturation desiccation appears to be a switch delineating a realm of expression for ZmGOLS3, predominant during seed development, from that of ZmGOLS2, principally associated with post-imbibition dehydration stress.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074310"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074310"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074310; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074309"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074309/Galactosyl_Cyclitols_and_Raffinose_Family_Oligosaccharides_in_Relation_to_Desiccation_Tolerance_of_Pea_and_Soyabean_Seedlings"><img alt="Research paper thumbnail of Galactosyl Cyclitols and Raffinose Family Oligosaccharides in Relation to Desiccation Tolerance of Pea and Soyabean Seedlings" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074309/Galactosyl_Cyclitols_and_Raffinose_Family_Oligosaccharides_in_Relation_to_Desiccation_Tolerance_of_Pea_and_Soyabean_Seedlings">Galactosyl Cyclitols and Raffinose Family Oligosaccharides in Relation to Desiccation Tolerance of Pea and Soyabean Seedlings</a></div><div class="wp-workCard_item"><span>Current plant science and biotechnology in agriculture</span><span>, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The loss of desiccation tolerance of pea and soyabean seedlings and associated changes in their s...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The loss of desiccation tolerance of pea and soyabean seedlings and associated changes in their soluble carbohydrates were studied. For the desiccation tolerance test, intact seedlings were dried rapidly at 12% relative humidity, or slowly in a series of decreasing relative humidities, followed by rehydration. Soluble carbohydrates were assayed before and after drying. Survival and regrowth of shoot, hypocotyl and root tissues were recorded. Soyabean hypocotyl and root tissues los t desiccation tolerance within 36 h during germination in association with the loss of raffinose, stachyose, galactopinitol A, galactopinitol B, ciceritol, and fagopyritol B1. In contrast, fructose, glucose and sorbitol accumulated. Sucrose levels increased during desiccation, but there was no effect of drying on changes of other soluble carbohydrates. Pea root tissues lost desiccation tolerance during the first 36 h of germination. Unlike roots, 80% of pea epicotyls survived the slow drying treatment and 40% survived the fast drying treatment of seedlings at up to 96 h after imbibition. Sucrose levels increased 5 to 10 fold in root and hypocotyl tissues and even more in epicotyls during desiccation. Glucose and fructose increased during germination and remained elevated after drying. The data indicate that monosaccharides may induce desiccation damage whereas oligosaccharides are not a prerequisite for desiccation tolerance of pea seedling epicotyls.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074309"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074309"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074309; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); 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</script> <div class="js-work-strip profile--work_container" data-work-id="122074308"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074308/Damage_and_tolerance_in_retrospect_and_prospect"><img alt="Research paper thumbnail of Damage and tolerance in retrospect and prospect" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074308/Damage_and_tolerance_in_retrospect_and_prospect">Damage and tolerance in retrospect and prospect</a></div><div class="wp-workCard_item"><span>CABI Publishing eBooks</span><span>, 2002</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074308"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074308"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074308; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074308]").text(description); $(".js-view-count[data-work-id=122074308]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074308; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074308']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074308]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074308,"title":"Damage and tolerance in retrospect and prospect","internal_url":"https://www.academia.edu/122074308/Damage_and_tolerance_in_retrospect_and_prospect","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074306"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074306/Recent_accomplishments_and_new_opportunities_in_seed_research"><img alt="Research paper thumbnail of Recent accomplishments and new opportunities in seed research" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074306/Recent_accomplishments_and_new_opportunities_in_seed_research">Recent accomplishments and new opportunities in seed research</a></div><div class="wp-workCard_item"><span>CABI Publishing eBooks</span><span>, 2003</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This chapter provides a brief introduction on the different breakthroughs regarding studies relat...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This chapter provides a brief introduction on the different breakthroughs regarding studies relating to seed research in Salamanca, Spain. These include: seed development; seed germination and dormancy; desiccation and other stress tolerance and conservation; seed ecology; seed banks and preservation of germplasm; and seed biotechnology. Also included are new opportunities for studies on seed biology.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074306"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074306"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074306; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074306]").text(description); $(".js-view-count[data-work-id=122074306]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074306; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074306']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074306]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074306,"title":"Recent accomplishments and new opportunities in seed research","internal_url":"https://www.academia.edu/122074306/Recent_accomplishments_and_new_opportunities_in_seed_research","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074305"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074305/Imbibitional_surface_damage_in_isolated_hypocotyl_root_axes_of_soybean_Glycine_max_L_Merr_cv_Chippewa_64_"><img alt="Research paper thumbnail of Imbibitional surface damage in isolated hypocotyl-root axes of soybean (Glycine max (L.) Merr. cv. Chippewa 64)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074305/Imbibitional_surface_damage_in_isolated_hypocotyl_root_axes_of_soybean_Glycine_max_L_Merr_cv_Chippewa_64_">Imbibitional surface damage in isolated hypocotyl-root axes of soybean (Glycine max (L.) Merr. cv. Chippewa 64)</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074305"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074305"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074305; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074305]").text(description); $(".js-view-count[data-work-id=122074305]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074305; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074305']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074305]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074305,"title":"Imbibitional surface damage in isolated hypocotyl-root axes of soybean (Glycine max (L.) Merr. cv. Chippewa 64)","internal_url":"https://www.academia.edu/122074305/Imbibitional_surface_damage_in_isolated_hypocotyl_root_axes_of_soybean_Glycine_max_L_Merr_cv_Chippewa_64_","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074304"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074304/Buckwheat_seed_set_in_planta_and_during_in_vitro_inflorescence_culture_evaluation_of_temperature_and_water_deficit_stress"><img alt="Research paper thumbnail of Buckwheat seed set in planta and during in vitro inflorescence culture: evaluation of temperature and water deficit stress" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074304/Buckwheat_seed_set_in_planta_and_during_in_vitro_inflorescence_culture_evaluation_of_temperature_and_water_deficit_stress">Buckwheat seed set in planta and during in vitro inflorescence culture: evaluation of temperature and water deficit stress</a></div><div class="wp-workCard_item"><span>Seed Science Research</span><span>, Apr 25, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT Common buckwheat (Fagopyrum esculentum Moench) plants produce many flowers, but fewer se...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT Common buckwheat (Fagopyrum esculentum Moench) plants produce many flowers, but fewer seeds. Seed set is highly variable among years, between plants, and during the period of flowering within a plant or raceme. Seasonal variations suggest that temperature and water-deficit stresses are important factors for seed set. The effects of mild temperature and water-deficit stresses on seed set and seed filling were determined in planta and in vitro. An in vitro method to culture matched sets of racemes from individual plants was used for precise comparisons between experimental treatments. Buckwheat racemes form new flowers continuously during several weeks in planta. Seed set resulting in yield occurs during the first 2–3 weeks of flowering and then rapidly declines in planta independently of mild stresses. Plants grown at 18°C have 40% increased seed set, set seeds over a longer duration, and produce 40% more dry matter per seed than plants grown at 25°C. Similar patterns occurred in vitro. A 3-day water-deficit stress during the first week of flowering reduced the number of seeds by 50%without a reduction in seed size and dry weight, or the number of flowers formed in planta or in vitro. The effect of water-deficit stress continued after rewatering and subsequently was expressed as a reduction in fertility in newly formed flowers, both in planta and in vitro. Mild temperature and water-deficit stresses affected both female and male components of seed set in common buckwheat, resulting in a persistent but non-additive reduction in sink strength.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074304"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074304"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074304; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074304]").text(description); $(".js-view-count[data-work-id=122074304]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074304; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074304']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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</script> <div class="js-work-strip profile--work_container" data-work-id="122074303"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074303/Free_cyclitol_unloading_from_seed_coats_on_stem_leaf_pod_explants_of_low_raffinose_low_stachyose_low_phytin_soybean"><img alt="Research paper thumbnail of Free cyclitol unloading from seed coats on stem–leaf–pod explants of low-raffinose, low-stachyose, low-phytin soybean" class="work-thumbnail" src="https://attachments.academia-assets.com/116809875/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074303/Free_cyclitol_unloading_from_seed_coats_on_stem_leaf_pod_explants_of_low_raffinose_low_stachyose_low_phytin_soybean">Free cyclitol unloading from seed coats on stem–leaf–pod explants of low-raffinose, low-stachyose, low-phytin soybean</a></div><div class="wp-workCard_item"><span>Seed Science Research</span><span>, Sep 24, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Raffinose, stachyose and phytin are undesirable compounds for soybean food and animal feed produc...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Raffinose, stachyose and phytin are undesirable compounds for soybean food and animal feed products. In seeds, raffinose and stachyose are believed to contribute to desiccation and cold stress tolerance. Thus, removal of these compounds from soybean by genetic mutation has resulted in a more commercially desirable composition, but potentially less physiologically viable seeds. In an effort to develop a method to improve viability and seed storability in soybean, stem-leaf-pod explants of three low raffinose, low stachyose lines, two of which were also low in phytin, and a check line were fed solutions containing D-chiro-inositol, myo-inositol or D-pinitol, free cyclitols which unload through the seed coat to the developing embryo where they accumulate as fagopyritols, galactinol and galactopinitols, respectively, during seed maturation. Increased galactopinitol and fagopyritol accumulation may substitute for the roles of raffinose and stachyose in low raffinose, stachyose and phytin seeds. Explants of all lines unloaded D-chiro-inositol, myo-inositol and D-pinitol. Fed D-chiro-inositol accumulated in leaf tissues demonstrating uptake into explants. Fed D-chiro-inositol and myo-inositol accumulated in pod wall and seed coat tissues of one or more lines. The results indicate that D-chiro-inositol was unloaded from the seed coat to the embryo in increased amounts after feeding. The potential use of increased maternal D-chiro-inositol for synthesis of fagopyritols in embryos to improve seed performance in low-stachyose and low-phytin soybean seeds is supported. The seed coat cup unloading of fed free cyclitols may provide a model system to test effective unloading of upregulated maternally synthesized cyclitols.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8759712d9adbea034df2de6ebe932f4e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809875,"asset_id":122074303,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809875/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074303"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074303"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074303; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074302"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074302/Soybean_Seed_Water_Relations_during_i_in_Situ_i_and_i_in_Vitro_i_Growth_and_Maturation"><img alt="Research paper thumbnail of Soybean Seed Water Relations during <i>in Situ</i> and <i>in Vitro</i> Growth and Maturation" class="work-thumbnail" src="https://attachments.academia-assets.com/116809847/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074302/Soybean_Seed_Water_Relations_during_i_in_Situ_i_and_i_in_Vitro_i_Growth_and_Maturation">Soybean Seed Water Relations during <i>in Situ</i> and <i>in Vitro</i> Growth and Maturation</a></div><div class="wp-workCard_item"><span>Plant Physiology</span><span>, Feb 1, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Water, osmotic, and pressure potentials of soybean (Glycine max [L.] Merrill) embryos and related...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Water, osmotic, and pressure potentials of soybean (Glycine max [L.] Merrill) embryos and related matemal tissues were measured during periods of seed growth and maturation to test the involvement of embryo water relations in seed maturation. Seeds were matured in situ or in an in vitro liquid culture medium in detached pods or as isolated seeds. Changes in water relations of embryo tissues were independent of maternal tissues. During seed maturation in situ, water and osmotic potentials in both embryo and maternal tissues declined sharply near the time of maximum dry weight. During in vitro seed culture with and without pods, water and osmotic potentials in axis and cotyledon tissues declined continuously during growth. Water and osmotic potentials of the seed coat, which was present only during in vitro seed culture with pods, changed little during the culture period. Positive turgor in the embryo was maintained beyond maximum dry weight and the loss of green color during in vitro culture but declined to zero at maturity in situ. The osmotic potential in embryo tissues declined from-1.1 megapascals at early pod fill to between-1.65 and-2.2 megapascals at maximum seed dry weight across all maturation environments. It is suggested that the decreasing osmotic potential in the growing soybean embryo reaches a threshold level that is associated with cessation of growth and onset of seed maturation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f9a74fb54553b8ef96ac44b1b883800f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809847,"asset_id":122074302,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809847/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074302"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074302"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074302; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074301"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074301/Polyribosome_formation_and_mRNA_synthesis_in_soybean_embryonic_axes_during_early_germination"><img alt="Research paper thumbnail of Polyribosome formation and mRNA synthesis in soybean embryonic axes during early germination" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074301/Polyribosome_formation_and_mRNA_synthesis_in_soybean_embryonic_axes_during_early_germination">Polyribosome formation and mRNA synthesis in soybean embryonic axes during early germination</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074301"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074301"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074301; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074301]").text(description); $(".js-view-count[data-work-id=122074301]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074301; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074301']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074301]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074301,"title":"Polyribosome formation and mRNA synthesis in soybean embryonic axes during early germination","internal_url":"https://www.academia.edu/122074301/Polyribosome_formation_and_mRNA_synthesis_in_soybean_embryonic_axes_during_early_germination","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074300"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074300/Relationship_of_Stelar_Lesions_to_Radicle_Growth_in_Corn_Seedlings_1"><img alt="Research paper thumbnail of Relationship of Stelar Lesions to Radicle Growth in Corn Seedlings 1" class="work-thumbnail" src="https://attachments.academia-assets.com/116923618/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074300/Relationship_of_Stelar_Lesions_to_Radicle_Growth_in_Corn_Seedlings_1">Relationship of Stelar Lesions to Radicle Growth in Corn Seedlings 1</a></div><div class="wp-workCard_item"><span>Agronomy Journal</span><span>, Nov 1, 1979</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Chilling during imbibition of Zcu mays L. kernels resulted in anatomical lesions acros the stele ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Chilling during imbibition of Zcu mays L. kernels resulted in anatomical lesions acros the stele of radicles, and subsequently in reduced growth of radicles. The objective was to determine if stelar lesion formation accounted for the radicle growth reduction. Inheritance patterns, 'source of seedstodrs, maturity of kernels, and surgical treatments of pericarp and endosperm were used to compare lesion occurrence and radicle growth in l a b ratory evaluations of field-grown seedstocks. Chilling injury of 5% (low) moisture kernels was measured as radicle growth reduction after a 48 hour hydration treatment at 5 C followed by 4 days at 25 C when compared to Similarly treated controls with 13% moisture kernels.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="72ef01276a3d55b1a7b02e9d8bf45e76" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116923618,"asset_id":122074300,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116923618/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074300"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074300"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074300; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074299"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074299/Purification_and_molecular_structure_of_digalactosyl_myo_inositol_DGMI_trigalactosyl_myo_inositol_TGMI_and_fagopyritol_B3_from_common_buckwheat_seeds_by_NMR"><img alt="Research paper thumbnail of Purification and molecular structure of digalactosyl myo-inositol (DGMI), trigalactosyl myo-inositol (TGMI), and fagopyritol B3 from common buckwheat seeds by NMR" class="work-thumbnail" src="https://attachments.academia-assets.com/116809876/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074299/Purification_and_molecular_structure_of_digalactosyl_myo_inositol_DGMI_trigalactosyl_myo_inositol_TGMI_and_fagopyritol_B3_from_common_buckwheat_seeds_by_NMR">Purification and molecular structure of digalactosyl myo-inositol (DGMI), trigalactosyl myo-inositol (TGMI), and fagopyritol B3 from common buckwheat seeds by NMR</a></div><div class="wp-workCard_item"><span>Carbohydrate Research</span><span>, Oct 1, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Three galactosyl cyclitols, digalactosyl myo-inositol (assigned the trivial name DGMI), trigalact...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Three galactosyl cyclitols, digalactosyl myo-inositol (assigned the trivial name DGMI), trigalactosyl myoinositol (assigned the trivial name TGMI), and trigalactosyl D-chiro-inositol (fagopyritol B3), were isolated from common buckwheat (Fagopyrum esculentum Moench) seeds. Structures of the three compounds were determined by 2D NMR spectroscopy. DGMI is aD -galactopyranosyl-(1?6)-aD -galactopyranosyl-(1?1)-1L-myo-inositol, TGMI is aD -galactopyranosyl-(1?6)-aD -galactopyranosyl-(1?6)-aD -galactopyranosyl-(1?1)-1L-myo-inositol, and fagopyritol B3 is aD -galactopyranosyl-(1?6)-aD -galactopyranosyl-(1? 6)-aD -galactopyranosyl-(1?2)-1D-chiro-inositol. DGMI and TGMI are higher oligomers of galactinol, a major galactosyl donor in plant seeds. Fagopyritol B3 is a higher oligomer of the fagopyritol B series and one of six fagopyritols found in buckwheat seeds and in the bran milling fraction. Samples of TGMI and fagopyritol B3 also contained the compounds N-(b-glucopyranosyl)-nicotinic acid and b-D-apiofuranosyl-(1?6)-b-D-(1-O-methyl)-glucopyranoside.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5014c340c177d8b61086d0acbd49b692" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809876,"asset_id":122074299,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809876/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074299"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074299"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074299; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074298"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074298/Soybean_seed_pectinesterase"><img alt="Research paper thumbnail of Soybean seed pectinesterase" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074298/Soybean_seed_pectinesterase">Soybean seed pectinesterase</a></div><div class="wp-workCard_item"><span>Seed Science Research</span><span>, Dec 1, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Methanol accumulates in axis tissues of maturing soybean seeds, correlating with preharvest seed ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Methanol accumulates in axis tissues of maturing soybean seeds, correlating with preharvest seed deterioration. Accumulation of methanol appears to be associated with the enzymic demethylation of pectin methyl esters by pectinesterase (PE; EC 3.1.1.11). To characterize PE in developing and maturing soybean (Glycine max (L.) Merrill) seeds, enzyme activity was assayed in axis and cotyledon tissues. Activity per g fresh weight was 20–25 times higher in axes than in cotyledons with highest activities between 45 and 60 days after flowering (DAF). Twenty to 33% of the total PE activity was in the ‘soluble’ form (extracted with water, 0.5 M sucrose, 1 M sucrose and water). Soluble and cell-wall-bound PE (subsequently extracted with 1 M NaCI) were purified and characterized from axes of seeds at 45–60 DAF. Purification of PE was achieved through concentration of extracts by ultra-filtration, precipitation with ammonium sulfate (30–80% saturation), dialysis, gel filtration on Sephadex G-75 columns, and ion exchange chromatography on CM Sepharose CL-6B. Further purification of both soluble and bound PE was by isoelectric focusing (IEF) on ultrathin layers of polyacrylamide gel with simultaneous detection of protein and PE activity. It was possible to follow seven bands exhibiting PE activity with pl values between 6.0 and 9.5 in 1 M NaCI-extracts of total homogenates. Differences in the IEF patterns of bound and soluble PE were observed. Whereas the bound enzyme exhibited more basic PE bands (pl 8–9.5), the soluble enzyme had more active bands at pl 6.5, 7.0 and 7.5. The Mr was close to 33 000 and the pH optimum was 7.8 for both soluble and bound PE.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074298"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074298"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074298; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074298]").text(description); $(".js-view-count[data-work-id=122074298]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074298; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074298']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074298]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074298,"title":"Soybean seed pectinesterase","internal_url":"https://www.academia.edu/122074298/Soybean_seed_pectinesterase","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074297"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074297/Soluble_sugars_and_flatulence_producing_oligosaccharides_in_maturing_yellow_lupin_i_Lupinus_luteus_i_L_seeds"><img alt="Research paper thumbnail of Soluble sugars and flatulence-producing oligosaccharides in maturing yellow lupin (<i>Lupinus luteus</i>L.) seeds" class="work-thumbnail" src="https://attachments.academia-assets.com/116809873/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074297/Soluble_sugars_and_flatulence_producing_oligosaccharides_in_maturing_yellow_lupin_i_Lupinus_luteus_i_L_seeds">Soluble sugars and flatulence-producing oligosaccharides in maturing yellow lupin (<i>Lupinus luteus</i>L.) seeds</a></div><div class="wp-workCard_item"><span>Seed Science Research</span><span>, Jun 1, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The flatulence-producing soluble oligosaccharides are an important component of lupin seeds and w...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The flatulence-producing soluble oligosaccharides are an important component of lupin seeds and were assayed to establish the pattern of their accumulation in relation to germinability during seed development and maturation. Maturing yellow lupin cv. Juno seeds were harvested at 5-day intervals from 15 to 45 days after flowering (DAF). Seed fresh mass increased to a maximum at 35 DAF followed by a decrease when axis and cotyledon tissues changed colour from green to yellow. Maximum seed fresh mass corresponded to the maximum seed size. Seed dry mass continuously increased until 40 DAF. About 75% of mature seed dry mass was in cotyledons, 22% in testa, and 3% in axis. Maximum seed germinability occurred at 45 DAF after maximum seed dry mass and desiccation. Maturing yellow lupin seeds were desiccation tolerant. Mature dry seeds contained 10.9% oligosaccharides and 1.5% sucrose. During seed growth stachyose accumulation preceded accumulation of raffinose and verbascose. The highest rate of ohgosaccharide accumulation appeared during seed desiccation and correlated with the acquisition of the ability to germinate. The sucrose :oligosaccharide ratio continuously decreased reaching 0.13 in mature seeds. Accumulation of oligosaccharides in maturing yellow lupin seeds is associated with seed germinability and seed desiccation tolerance.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d1b27e0af8737ddd6e78c306988321ff" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809873,"asset_id":122074297,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809873/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074297"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074297"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074297; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074296"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074296/Purification_and_molecular_structure_of_two_digalactosyl_d_chiro_inositols_and_two_trigalactosyl_d_chiro_inositols_from_buckwheat_seeds"><img alt="Research paper thumbnail of Purification and molecular structure of two digalactosyl d-chiro-inositols and two trigalactosyl d-chiro-inositols from buckwheat seeds" class="work-thumbnail" src="https://attachments.academia-assets.com/116809878/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074296/Purification_and_molecular_structure_of_two_digalactosyl_d_chiro_inositols_and_two_trigalactosyl_d_chiro_inositols_from_buckwheat_seeds">Purification and molecular structure of two digalactosyl d-chiro-inositols and two trigalactosyl d-chiro-inositols from buckwheat seeds</a></div><div class="wp-workCard_item"><span>Carbohydrate Research</span><span>, Mar 1, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Two digalactosyl D-chiro-inositols and two trigalactosyl D-chiro-inositols, members of the fagopy...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Two digalactosyl D-chiro-inositols and two trigalactosyl D-chiro-inositols, members of the fagopyritol A series and fagopyritol B series, were isolated from buckwheat (Fagopyrum esculentum Moench) seeds. Structures of the first three were determined by 1 H and 13 C NMR. Fagopyritol B2 is aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 2)-1D-chiro-inositol, and fagopyritol A2 is aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 3)-1D-chiroinositol. Fagopyritol A3, a trigalactosyl D-chiro-inositol, is aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 3)-1D-chiro-inositol. From analysis of hydrolysis products, the second trigalactosyl D-chiro-inositol, fagopyritol B3, is aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 6)-aD -galactopyranosyl-(1 2)-1D-chiro-inositol.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="094b88997d774882ebef9706c20d1f3e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809878,"asset_id":122074296,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809878/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074296"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074296"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074296; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074295"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074295/Quantitative_Assessment_of_Some_Factors_Limiting_Seed_Set_in_Buckwheat"><img alt="Research paper thumbnail of Quantitative Assessment of Some Factors Limiting Seed Set in Buckwheat" class="work-thumbnail" src="https://attachments.academia-assets.com/116809872/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074295/Quantitative_Assessment_of_Some_Factors_Limiting_Seed_Set_in_Buckwheat">Quantitative Assessment of Some Factors Limiting Seed Set in Buckwheat</a></div><div class="wp-workCard_item"><span>Crop Science</span><span>, Nov 1, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">estimate of fitness, for example, seed set. Likewise, the theory of reproductive strategies of pl...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">estimate of fitness, for example, seed set. Likewise, the theory of reproductive strategies of plants has been in-A healthy buckwheat (Fagopyrum esculentum Moench) plant profluenced by our ability to determine the mechanisms by duces numerous flowers but few seeds. The objective of this study was to investigate mechanisms responsible for the low frequency of which selection may occur. seed set. Newly open and receptive flowers were pollinated at weekly Reduced seed number can be caused by: (i) insuffiintervals from 4 to 12 wk after seeding (WAS) on selected racemes cient pollination, (ii) sexual selection of male and female of greenhouse-grown plants. Flower number, seed set, abortion, and gametophytes, and (iii) resource limitation of the materanatomical observations of egg sacs and proembryos were analyzed. nal plant (Bawa and Webb, 1984). The male component Although flowers formed continuously for 7 wk, the period of maxiof fitness has been assessed in Campsis radicans (Bertin, mum seed set under controlled conditions was brief (1-2 wk). Compe-1982), Calathea ovandensis (Horvitz and Schemske, 1988), tition within the same nutritional unit (raceme) did not account for Cassia fasiculata (Lee and Bazzaz, 1982), Agave mckellow average seed set per raceme. Neither the number of flowers nor veyana (Sutherland and Delph, 1984), and Lithopserfrequency of seed set was decreased when developing seeds were on mum caroliniense (Weller, 1980). In some cases the evithe same raceme. Abortion (≈10%) was not a major factor limiting seed set. Eighty percent of the flowers failed to initiate seeds even dence indicates that excess flowers contribute to male under controlled growing conditions when hand pollinated. Approxi-fitness by acting as pollen donors (Agave), while in mately 20% of megagametophytes appeared to be defective at antheother cases (Cassia, Lithospermum, Calathea, Campsis) sis. The proportion of ovules containing a proembryo at 24 h after it does not. Sutherland (1987) pooled published obserpollination (HAP) paralleled the proportion initiating seeds, indicatvations of 445 species to test hypotheses related to male ing that fertilization is a limiting process. Pollen tube remnants were fitness. He concluded that excess flowers that fail to observed near the micropyle in 65% of the unfertilized ovules at 24 form mature fruits do not contribute to female fitness HAP. Lack of fertilization accounts for most of the reduction in seed (fruit or seed set) because they abort regardless of their set, appears to be caused by failure of the pollen tube to grow into pollination history. They may act as pollen donors, howthe micropyle, and increases with plant age at pollination. Flowers ever, and thus contribute to male fitness. Conditions for that form late in the plant cycle contribute little to seed set and may be ignored in yield management decisions.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="86ae08eb3149a87fb3b554358c05b16d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809872,"asset_id":122074295,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809872/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074295"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074295"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074295; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074294"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/122074294/Raffinose_accumulation_in_maize_embryos_in_the_absence_of_a_fully_functional_Vp1_gene_product"><img alt="Research paper thumbnail of Raffinose accumulation in maize embryos in the absence of a fully functional Vp1 gene product" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/122074294/Raffinose_accumulation_in_maize_embryos_in_the_absence_of_a_fully_functional_Vp1_gene_product">Raffinose accumulation in maize embryos in the absence of a fully functional Vp1 gene product</a></div><div class="wp-workCard_item"><span>Planta</span><span>, Sep 26, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">... type kernels segre-gated on the same ears (Vp1-1695, purple) also were desiccation tolerant i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... type kernels segre-gated on the same ears (Vp1-1695, purple) also were desiccation tolerant in association with a sucrose:raffi-nose mass ... vp1-McWhirter, vp1-A1, and vp1-C821708 were produced at Homestead, Fla., USA during winter 1993/1994 and at Aurora, NY, USA in ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074294"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074294"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074294; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=122074294]").text(description); $(".js-view-count[data-work-id=122074294]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 122074294; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='122074294']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=122074294]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":122074294,"title":"Raffinose accumulation in maize embryos in the absence of a fully functional Vp1 gene product","internal_url":"https://www.academia.edu/122074294/Raffinose_accumulation_in_maize_embryos_in_the_absence_of_a_fully_functional_Vp1_gene_product","owner_id":43864210,"coauthors_can_edit":true,"owner":{"id":43864210,"first_name":"Ralph","middle_initials":null,"last_name":"Obendorf","page_name":"RalphObendorf","domain_name":"independent","created_at":"2016-02-24T11:35:41.696-08:00","display_name":"Ralph Obendorf","url":"https://independent.academia.edu/RalphObendorf"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074293"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074293/Influence_of_Age_and_Illumination_on_Distribution_of_Several_Calvin_Cycle_Enzymes_in_Greening_Barley_Leaves"><img alt="Research paper thumbnail of Influence of Age and Illumination on Distribution of Several Calvin Cycle Enzymes in Greening Barley Leaves" class="work-thumbnail" src="https://attachments.academia-assets.com/116809871/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074293/Influence_of_Age_and_Illumination_on_Distribution_of_Several_Calvin_Cycle_Enzymes_in_Greening_Barley_Leaves">Influence of Age and Illumination on Distribution of Several Calvin Cycle Enzymes in Greening Barley Leaves</a></div><div class="wp-workCard_item"><span>Plant Physiology</span><span>, May 1, 1970</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Activities of phosphoriboisomerase, phosphoribulokinase, and ribulose 1,5-diphosphate carboxylase...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Activities of phosphoriboisomerase, phosphoribulokinase, and ribulose 1,5-diphosphate carboxylase, protein content, and chlorophyll accumulation in dark-grown barley seedlings were measured before and after illumination. Enzymatic activities, levels of soluble protein, and accumulation (upon illumination) of chlorophyll in leaves declined from tips toward the base. In response to increasing time of illumination, chlorophyll accumulation and activities of phosphoribulokinase and ribulose 1 ,5-diphosphate carboxylase (enzymes located in chloroplasts) increased most in tip portions whereas activity of phosphoriboisomerase and levels of soluble protein (constituents not confined to chloroplasts) increased similarly in all sections of the leaf. Maximum activity of phosphoribulokinase and maximum accumulation of chlorophyll shifted toward median portions of the leaf blade with increased age of seedling before illumination. Maximum activity of ribulose 1 ,5-diphosphate carboxylase and maximum level of soluble protein occurred in all leaf sections when the seedlings were 7 days of age before illumination.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="396db83af3286d4989364dcb6b86a299" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809871,"asset_id":122074293,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809871/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074293"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074293"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074293; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074292"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074292/Soluble_carbohydrates_in_mature_soybean_seed_after_feeding_d_chiro_inositol_myo_inositol_or_d_pinitol_to_stem_leaf_pod_explants_of_low_raffinose_low_stachyose_lines"><img alt="Research paper thumbnail of Soluble carbohydrates in mature soybean seed after feeding d-chiro-inositol, myo-inositol, or d-pinitol to stem-leaf-pod explants of low-raffinose, low-stachyose lines" class="work-thumbnail" src="https://attachments.academia-assets.com/116809874/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074292/Soluble_carbohydrates_in_mature_soybean_seed_after_feeding_d_chiro_inositol_myo_inositol_or_d_pinitol_to_stem_leaf_pod_explants_of_low_raffinose_low_stachyose_lines">Soluble carbohydrates in mature soybean seed after feeding d-chiro-inositol, myo-inositol, or d-pinitol to stem-leaf-pod explants of low-raffinose, low-stachyose lines</a></div><div class="wp-workCard_item"><span>Plant Science</span><span>, Nov 1, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="03689b79b703f5ab9a35e534583abfe8" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809874,"asset_id":122074292,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809874/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074292"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074292"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074292; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="122074226"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/122074226/Effect_of_Seed_Moisture_on_Temperature_Sensitivity_During_Imbibition_of_Soybean_sup_1_sup_"><img alt="Research paper thumbnail of Effect of Seed Moisture on Temperature Sensitivity During Imbibition of Soybean <sup>1</sup>" class="work-thumbnail" src="https://attachments.academia-assets.com/116809802/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/122074226/Effect_of_Seed_Moisture_on_Temperature_Sensitivity_During_Imbibition_of_Soybean_sup_1_sup_">Effect of Seed Moisture on Temperature Sensitivity During Imbibition of Soybean <sup>1</sup></a></div><div class="wp-workCard_item"><span>Crop Science</span><span>, Sep 1, 1970</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Seeds of 12 soybean {Glycine max (L.) Merr.} varieties were equilibrated to 6% or 16% moisture be...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Seeds of 12 soybean {Glycine max (L.) Merr.} varieties were equilibrated to 6% or 16% moisture before imbibition at cold (5 C) temperature, warm (25 C) temperature, or warm followed by exposure to cold. After imbibition, seeds were transferred to prewetted autoclaved soil mix in a greenhouse for 2 weeks. The number of seedlings surviving, height to apical bud, and dry weight of aerial parts without cotyledons were determined. Imbibition of low moisture seeds at 5 C caused reduction in survival, dry matter accumulation, and height of seedlings, but no reduction was observed with high moisture seeds. When low moisture seeds were imbibed at warm temperature before exposure to cold, no reduction was noted. The effect of initial seed moisture on sensitivity to chilling during imbibition was reversible. While some varieties were tolerant to cold imbibition of low moisture seeds, other varieties had up to 75% reduction in survival, 38% reduction in dry matter accumulation per seedling, and 50% reduction in plant height when compared to warm imbibition. Imbibition treatments which resulted in greatest seedling injury also decreased the capacity of seedlings to utilize cotyledonary reserves. Our results suggest that adjusting seed moisture to 16% before seeding could improve seedling establishment of soybean in cold soil.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="33bd126ecc084d1a679ddc49f6b9d3fd" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":116809802,"asset_id":122074226,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/116809802/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="122074226"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="122074226"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 122074226; 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