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-webkit-line-clamp: 3; -webkit-box-orient: vertical; }</style><div class="col-xs-12 clearfix"><div class="u-floatLeft"><h1 class="PageHeader-title u-m0x u-fs30">DNA fragmentation</h1><div class="u-tcGrayDark">23 Followers</div><div class="u-tcGrayDark u-mt2x">Recent papers in <b>DNA fragmentation</b></div></div></div></div></div></div><div class="TabbedNavigation"><div class="container"><div class="row"><div class="col-xs-12 clearfix"><ul class="nav u-m0x u-p0x list-inline u-displayFlex"><li class="active"><a href="https://www.academia.edu/Documents/in/DNA_fragmentation">Top Papers</a></li><li><a href="https://www.academia.edu/Documents/in/DNA_fragmentation/MostCited">Most Cited Papers</a></li><li><a href="https://www.academia.edu/Documents/in/DNA_fragmentation/MostDownloaded">Most Downloaded Papers</a></li><li><a href="https://www.academia.edu/Documents/in/DNA_fragmentation/MostRecent">Newest Papers</a></li><li><a class="" 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Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="38650" href="https://www.academia.edu/Documents/in/Cell_Division">Cell Division</a>, <script data-card-contents-for-ri="38650" type="text/json">{"id":38650,"name":"Cell Division","url":"https://www.academia.edu/Documents/in/Cell_Division?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="57808" href="https://www.academia.edu/Documents/in/Cell_line">Cell line</a><script data-card-contents-for-ri="57808" type="text/json">{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22262678]'), work: {"id":22262678,"title":"Insulin-like Growth Factor Binding Protein-related Protein 1 Inhibits Proliferation of 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href="https://www.academia.edu/Documents/in/DNA_fragmentation">DNA fragmentation</a>, <script data-card-contents-for-ri="2256666" type="text/json">{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2274872" href="https://www.academia.edu/Documents/in/DNA_sequence">DNA sequence</a><script data-card-contents-for-ri="2274872" type="text/json">{"id":2274872,"name":"DNA sequence","url":"https://www.academia.edu/Documents/in/DNA_sequence?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21652263]'), work: {"id":21652263,"title":"Construction of aCColE1 plasmid bearing inducible high-copy-number 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href="https://www.academia.edu/Documents/in/Urology">Urology</a>, <script data-card-contents-for-ri="653" type="text/json">{"id":653,"name":"Urology","url":"https://www.academia.edu/Documents/in/Urology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a>, <script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="75318" href="https://www.academia.edu/Documents/in/Smoking">Smoking</a><script 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{ var workId = 29291049; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_29291049"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_29291049 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="29291049"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 29291049; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=29291049]").text(description); $(".js-view-count-work_29291049").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_29291049").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="29291049"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3471" href="https://www.academia.edu/Documents/in/Regeneration">Regeneration</a>, <script data-card-contents-for-ri="3471" type="text/json">{"id":3471,"name":"Regeneration","url":"https://www.academia.edu/Documents/in/Regeneration?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="66089" href="https://www.academia.edu/Documents/in/Tissue_culture">Tissue culture</a>, <script data-card-contents-for-ri="66089" type="text/json">{"id":66089,"name":"Tissue culture","url":"https://www.academia.edu/Documents/in/Tissue_culture?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="113903" href="https://www.academia.edu/Documents/in/Bacteria">Bacteria</a><script data-card-contents-for-ri="113903" type="text/json">{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=29291049]'), work: {"id":29291049,"title":"Transgenic almond ( Prunus dulcis Mill.) plants obtained by Agrobacterium -mediated transformation of leaf explants","created_at":"2016-10-20T01:43:49.491-07:00","url":"https://www.academia.edu/29291049/Transgenic_almond_Prunus_dulcis_Mill_plants_obtained_by_Agrobacterium_mediated_transformation_of_leaf_explants?f_ri=2256666","dom_id":"work_29291049","summary":null,"downloadable_attachments":[{"id":49734442,"asset_id":29291049,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38325685,"first_name":"Célia","last_name":"Miguel","domain_name":"lisboa","page_name":"CéliaMiguel","display_name":"Célia Miguel","profile_url":"https://lisboa.academia.edu/C%C3%A9liaMiguel?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3471,"name":"Regeneration","url":"https://www.academia.edu/Documents/in/Regeneration?f_ri=2256666","nofollow":false},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=2256666","nofollow":false},{"id":66089,"name":"Tissue culture","url":"https://www.academia.edu/Documents/in/Tissue_culture?f_ri=2256666","nofollow":false},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=2256666","nofollow":false},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=2256666"},{"id":143509,"name":"Genetic transformation","url":"https://www.academia.edu/Documents/in/Genetic_transformation?f_ri=2256666"},{"id":394053,"name":"Chimera","url":"https://www.academia.edu/Documents/in/Chimera?f_ri=2256666"},{"id":1706341,"name":"Gene Transfer","url":"https://www.academia.edu/Documents/in/Gene_Transfer?f_ri=2256666"},{"id":1916668,"name":"Indole-3-butyric Acid","url":"https://www.academia.edu/Documents/in/Indole-3-butyric_Acid?f_ri=2256666"},{"id":2210888,"name":"Transient Expression","url":"https://www.academia.edu/Documents/in/Transient_Expression?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"},{"id":2398601,"name":"Indole Acetic Acid","url":"https://www.academia.edu/Documents/in/Indole_Acetic_Acid?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_72255856" data-work_id="72255856" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/72255856/Type_III_secretion_mediated_killing_of_endothelial_cells_by_Pseudomonas_aeruginosa">Type III secretion-mediated killing of endothelial cells by Pseudomonas aeruginosa</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Pseudomonas aeruginosa, a common agent of septicemia, enters into human endothelial cellsin vitro but the effects of bacterial infection have not been addressed properly. In this study, human umbilical vein endothelial cells (HUVEC) were... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_72255856" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Pseudomonas aeruginosa, a common agent of septicemia, enters into human endothelial cellsin vitro but the effects of bacterial infection have not been addressed properly. In this study, human umbilical vein endothelial cells (HUVEC) were infected by the noninvasive PA103 and ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/72255856" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="83455c4855aab311c345768eb3bf18bb" rel="nofollow" data-download="{"attachment_id":81255894,"asset_id":72255856,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/81255894/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50666871" href="https://independent.academia.edu/AssisMariaCristina">Maria Cristina Assis</a><script data-card-contents-for-user="50666871" type="text/json">{"id":50666871,"first_name":"Maria Cristina","last_name":"Assis","domain_name":"independent","page_name":"AssisMariaCristina","display_name":"Maria Cristina Assis","profile_url":"https://independent.academia.edu/AssisMariaCristina?f_ri=2256666","photo":"https://0.academia-photos.com/50666871/40324183/33064283/s65_maria_cristina.assis.jpg"}</script></span></span></li><li class="js-paper-rank-work_72255856 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="72255856"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 72255856, container: ".js-paper-rank-work_72255856", }); 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$(".js-view-count[data-work-id=72255856]").text(description); $(".js-view-count-work_72255856").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_72255856").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="72255856"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="10937" href="https://www.academia.edu/Documents/in/Transcription_Regulation">Transcription Regulation</a><script data-card-contents-for-ri="10937" type="text/json">{"id":10937,"name":"Transcription Regulation","url":"https://www.academia.edu/Documents/in/Transcription_Regulation?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=72255856]'), work: {"id":72255856,"title":"Type III secretion-mediated killing of endothelial cells by Pseudomonas aeruginosa","created_at":"2022-02-22T13:58:09.482-08:00","url":"https://www.academia.edu/72255856/Type_III_secretion_mediated_killing_of_endothelial_cells_by_Pseudomonas_aeruginosa?f_ri=2256666","dom_id":"work_72255856","summary":"Pseudomonas aeruginosa, a common agent of septicemia, enters into human endothelial cellsin vitro but the effects of bacterial infection have not been addressed properly. In this study, human umbilical vein endothelial cells (HUVEC) were infected by the noninvasive PA103 and ...","downloadable_attachments":[{"id":81255894,"asset_id":72255856,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50666871,"first_name":"Maria Cristina","last_name":"Assis","domain_name":"independent","page_name":"AssisMariaCristina","display_name":"Maria Cristina Assis","profile_url":"https://independent.academia.edu/AssisMariaCristina?f_ri=2256666","photo":"https://0.academia-photos.com/50666871/40324183/33064283/s65_maria_cristina.assis.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=2256666","nofollow":false},{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=2256666","nofollow":false},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=2256666","nofollow":false},{"id":10937,"name":"Transcription Regulation","url":"https://www.academia.edu/Documents/in/Transcription_Regulation?f_ri=2256666","nofollow":false},{"id":15719,"name":"Mitochondria","url":"https://www.academia.edu/Documents/in/Mitochondria?f_ri=2256666"},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=2256666"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666"},{"id":88062,"name":"Vasculitis","url":"https://www.academia.edu/Documents/in/Vasculitis?f_ri=2256666"},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death?f_ri=2256666"},{"id":138877,"name":"Vascular endothelium","url":"https://www.academia.edu/Documents/in/Vascular_endothelium?f_ri=2256666"},{"id":159742,"name":"Microbial","url":"https://www.academia.edu/Documents/in/Microbial?f_ri=2256666"},{"id":175430,"name":"Microbial Pathogenesis","url":"https://www.academia.edu/Documents/in/Microbial_Pathogenesis?f_ri=2256666"},{"id":225499,"name":"Pseudomonas aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_aeruginosa?f_ri=2256666"},{"id":401214,"name":"Endothelial cell","url":"https://www.academia.edu/Documents/in/Endothelial_cell?f_ri=2256666"},{"id":636395,"name":"Pseudomonas Aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_Aeruginosa-1?f_ri=2256666"},{"id":1174994,"name":"Bacterial Infection","url":"https://www.academia.edu/Documents/in/Bacterial_Infection?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"},{"id":2272583,"name":"MTT assay","url":"https://www.academia.edu/Documents/in/MTT_assay?f_ri=2256666"},{"id":2467505,"name":"Gentamicins","url":"https://www.academia.edu/Documents/in/Gentamicins?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_48496023" data-work_id="48496023" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48496023/Vitrification_of_neat_semen_alters_sperm_parameters_and_DNA_integrity">Vitrification of neat semen alters sperm parameters and DNA integrity</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Our aim was to evaluate the effect of neat semen vitrification on human sperm vital parameters and DNA integrity in men with normal and abnormal sperm parameters. Semen samples were 17 normozoospermic samples and 17 specimens with... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48496023" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Our aim was to evaluate the effect of neat semen vitrification on human sperm vital parameters and DNA integrity in men with normal and abnormal sperm parameters. Semen samples were 17 normozoospermic samples and 17 specimens with abnormal sperm parameters. Semen analysis was performed according to World Health Organization (WHO) criteria. Then, the smear was provided from each sample and fixed for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Vitrification of neat semen was done by plunging cryoloops directly into liquid nitrogen and preserved for 7 days. The samples were warmed and re-evaluated for sperm parameters as well as DNA integrity. Besides, the correlation between sperm parameters and DNA fragmentation was assessed pre- and post vitrification. Cryopreserved spermatozoa showed significant decrease in sperm motility, viability and normal morphology after thawing in both normal and abnormal semen. Also, the rate of sperm DNA fragmentation was...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48496023" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3f4119e7f455c9c150d7e772f5bdb92d" rel="nofollow" data-download="{"attachment_id":67077615,"asset_id":48496023,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/67077615/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="141609963" href="https://independent.academia.edu/ImanHalvaei">Iman Halvaei</a><script data-card-contents-for-user="141609963" type="text/json">{"id":141609963,"first_name":"Iman","last_name":"Halvaei","domain_name":"independent","page_name":"ImanHalvaei","display_name":"Iman Halvaei","profile_url":"https://independent.academia.edu/ImanHalvaei?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_48496023 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48496023"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48496023, container: ".js-paper-rank-work_48496023", }); 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$(".js-view-count[data-work-id=48496023]").text(description); $(".js-view-count-work_48496023").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_48496023").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="48496023"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="653" href="https://www.academia.edu/Documents/in/Urology">Urology</a>, <script data-card-contents-for-ri="653" type="text/json">{"id":653,"name":"Urology","url":"https://www.academia.edu/Documents/in/Urology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a>, <script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111545" href="https://www.academia.edu/Documents/in/Male">Male</a>, <script data-card-contents-for-ri="111545" type="text/json">{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="141898" href="https://www.academia.edu/Documents/in/Semen_Analysis">Semen Analysis</a><script data-card-contents-for-ri="141898" type="text/json">{"id":141898,"name":"Semen Analysis","url":"https://www.academia.edu/Documents/in/Semen_Analysis?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48496023]'), work: {"id":48496023,"title":"Vitrification of neat semen alters sperm parameters and DNA integrity","created_at":"2021-05-04T21:09:45.282-07:00","url":"https://www.academia.edu/48496023/Vitrification_of_neat_semen_alters_sperm_parameters_and_DNA_integrity?f_ri=2256666","dom_id":"work_48496023","summary":"Our aim was to evaluate the effect of neat semen vitrification on human sperm vital parameters and DNA integrity in men with normal and abnormal sperm parameters. Semen samples were 17 normozoospermic samples and 17 specimens with abnormal sperm parameters. Semen analysis was performed according to World Health Organization (WHO) criteria. Then, the smear was provided from each sample and fixed for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Vitrification of neat semen was done by plunging cryoloops directly into liquid nitrogen and preserved for 7 days. The samples were warmed and re-evaluated for sperm parameters as well as DNA integrity. Besides, the correlation between sperm parameters and DNA fragmentation was assessed pre- and post vitrification. Cryopreserved spermatozoa showed significant decrease in sperm motility, viability and normal morphology after thawing in both normal and abnormal semen. Also, the rate of sperm DNA fragmentation was...","downloadable_attachments":[{"id":67077615,"asset_id":48496023,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":141609963,"first_name":"Iman","last_name":"Halvaei","domain_name":"independent","page_name":"ImanHalvaei","display_name":"Iman Halvaei","profile_url":"https://independent.academia.edu/ImanHalvaei?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":653,"name":"Urology","url":"https://www.academia.edu/Documents/in/Urology?f_ri=2256666","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=2256666","nofollow":false},{"id":141898,"name":"Semen Analysis","url":"https://www.academia.edu/Documents/in/Semen_Analysis?f_ri=2256666","nofollow":false},{"id":295155,"name":"Middle Aged","url":"https://www.academia.edu/Documents/in/Middle_Aged?f_ri=2256666"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult?f_ri=2256666"},{"id":448182,"name":"Vitrification","url":"https://www.academia.edu/Documents/in/Vitrification?f_ri=2256666"},{"id":781610,"name":"Semen","url":"https://www.academia.edu/Documents/in/Semen?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_2081812" data-work_id="2081812" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/2081812/A_method_to_size_DNA_fragments_from_50_to_800_bp_on_a_DNA_analyser">A method to size DNA fragments from 50 to 800 bp on a DNA analyser</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/2081812" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6bb5b755fe877382cab068faacc19ac2" rel="nofollow" data-download="{"attachment_id":50769959,"asset_id":2081812,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50769959/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="2717901" href="https://fieldmuseum.academia.edu/KevinFeldheim">Kevin Feldheim</a><script data-card-contents-for-user="2717901" type="text/json">{"id":2717901,"first_name":"Kevin","last_name":"Feldheim","domain_name":"fieldmuseum","page_name":"KevinFeldheim","display_name":"Kevin Feldheim","profile_url":"https://fieldmuseum.academia.edu/KevinFeldheim?f_ri=2256666","photo":"https://0.academia-photos.com/2717901/875067/1092331/s65_kevin.feldheim.jpg"}</script></span></span></li><li class="js-paper-rank-work_2081812 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="2081812"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 2081812, container: ".js-paper-rank-work_2081812", }); 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$(".js-view-count[data-work-id=2081812]").text(description); $(".js-view-count-work_2081812").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_2081812").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="2081812"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">3</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="15375" href="https://www.academia.edu/Documents/in/Capillary_electrophoresis">Capillary electrophoresis</a>, <script data-card-contents-for-ri="15375" type="text/json">{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2256666" href="https://www.academia.edu/Documents/in/DNA_fragmentation">DNA fragmentation</a><script data-card-contents-for-ri="2256666" type="text/json">{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=2081812]'), work: {"id":2081812,"title":"A method to size DNA fragments from 50 to 800 bp on a DNA analyser","created_at":"2012-11-02T14:34:54.388-07:00","url":"https://www.academia.edu/2081812/A_method_to_size_DNA_fragments_from_50_to_800_bp_on_a_DNA_analyser?f_ri=2256666","dom_id":"work_2081812","summary":null,"downloadable_attachments":[{"id":50769959,"asset_id":2081812,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":2717901,"first_name":"Kevin","last_name":"Feldheim","domain_name":"fieldmuseum","page_name":"KevinFeldheim","display_name":"Kevin Feldheim","profile_url":"https://fieldmuseum.academia.edu/KevinFeldheim?f_ri=2256666","photo":"https://0.academia-photos.com/2717901/875067/1092331/s65_kevin.feldheim.jpg"}],"research_interests":[{"id":15375,"name":"Capillary electrophoresis","url":"https://www.academia.edu/Documents/in/Capillary_electrophoresis?f_ri=2256666","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=2256666","nofollow":false},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_8108653" data-work_id="8108653" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/8108653/Melatonin_reduces_apoptosis_and_necrosis_induced_by_ischemia_reperfusion_injury_of_the_pancreas">Melatonin reduces apoptosis and necrosis induced by ischemia/reperfusion injury of the pancreas</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Abstract: The pancreas is highly susceptible to the oxidative stress induced by ischemia/reperfusion (IR) injury leading to the generation of acute pancreatitis. Melatonin has been shown to be useful in the prevention of the damage by... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_8108653" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Abstract: The pancreas is highly susceptible to the oxidative stress induced by ischemia/reperfusion (IR) injury leading to the generation of acute pancreatitis. Melatonin has been shown to be useful in the prevention of the damage by ischemia-reperfusion in liver, brain, myocardium, gut and kidney. The aim of the study was to evaluate the cytoprotective properties of melatonin against injury induced by IR in pancreas. The obstruction of gastro-duodenal and inferior splenic arteries induced pancreatic IR in male Wistar rats. Melatonin was intraperitoneally administered before or/and after IR injury. The animals were killed at 24 and 48 hr after reperfusion and there were evaluated parameters of oxidative stress (lipoperoxides, superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione), glandular endocrine and exocrine function (lipase, amylase, insulin) and cell injury (apoptosis and necrosis). The IR induced a marked enhancement of oxidative stress and impaired pancreatic function. The histological analysis showed that IR induced acute pancreatitis with the accumulation of inflammatory infiltrate, disruption of tissue structure, cell necrosis and hemorrhage. Melatonin administration before or after pancreatic IR prevented all tissue markers of oxidative stress, biochemical and histological signs of apoptosis and necrosis, and restored glandular function. No histological signs of pancreatitis were observed 48 hr after reperfusion in 80% of the animals treated with melatonin, with only a mild edematous pancreatitis being observed in the remaining rats. Preventive or therapeutic administration of melatonin protected against the induction of oxidative stress and tissue injury, and restored cell function in experimental pancreatic IR in rats.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/8108653" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ced8b452d4d8f6a577c74511e79f4f17" rel="nofollow" data-download="{"attachment_id":48212173,"asset_id":8108653,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48212173/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="15733553" href="https://independent.academia.edu/CARLOSPERA">CARLOS PERA</a><script data-card-contents-for-user="15733553" type="text/json">{"id":15733553,"first_name":"CARLOS","last_name":"PERA","domain_name":"independent","page_name":"CARLOSPERA","display_name":"CARLOS PERA","profile_url":"https://independent.academia.edu/CARLOSPERA?f_ri=2256666","photo":"https://0.academia-photos.com/15733553/162642838/152413227/s65_carlos.pera.png"}</script></span></span></li><li class="js-paper-rank-work_8108653 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="8108653"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 8108653, container: ".js-paper-rank-work_8108653", }); 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$(".js-view-count[data-work-id=8108653]").text(description); $(".js-view-count-work_8108653").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_8108653").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="8108653"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">24</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="21143" href="https://www.academia.edu/Documents/in/Pancreatitis">Pancreatitis</a>, <script data-card-contents-for-ri="21143" type="text/json">{"id":21143,"name":"Pancreatitis","url":"https://www.academia.edu/Documents/in/Pancreatitis?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="24731" href="https://www.academia.edu/Documents/in/Apoptosis">Apoptosis</a>, <script data-card-contents-for-ri="24731" type="text/json">{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="50841" href="https://www.academia.edu/Documents/in/Caspases">Caspases</a><script data-card-contents-for-ri="50841" type="text/json">{"id":50841,"name":"Caspases","url":"https://www.academia.edu/Documents/in/Caspases?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=8108653]'), work: {"id":8108653,"title":"Melatonin reduces apoptosis and necrosis induced by ischemia/reperfusion injury of the pancreas","created_at":"2014-08-27T19:03:45.232-07:00","url":"https://www.academia.edu/8108653/Melatonin_reduces_apoptosis_and_necrosis_induced_by_ischemia_reperfusion_injury_of_the_pancreas?f_ri=2256666","dom_id":"work_8108653","summary":"Abstract: The pancreas is highly susceptible to the oxidative stress induced by ischemia/reperfusion (IR) injury leading to the generation of acute pancreatitis. Melatonin has been shown to be useful in the prevention of the damage by ischemia-reperfusion in liver, brain, myocardium, gut and kidney. The aim of the study was to evaluate the cytoprotective properties of melatonin against injury induced by IR in pancreas. The obstruction of gastro-duodenal and inferior splenic arteries induced pancreatic IR in male Wistar rats. Melatonin was intraperitoneally administered before or/and after IR injury. The animals were killed at 24 and 48 hr after reperfusion and there were evaluated parameters of oxidative stress (lipoperoxides, superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione), glandular endocrine and exocrine function (lipase, amylase, insulin) and cell injury (apoptosis and necrosis). The IR induced a marked enhancement of oxidative stress and impaired pancreatic function. The histological analysis showed that IR induced acute pancreatitis with the accumulation of inflammatory infiltrate, disruption of tissue structure, cell necrosis and hemorrhage. Melatonin administration before or after pancreatic IR prevented all tissue markers of oxidative stress, biochemical and histological signs of apoptosis and necrosis, and restored glandular function. No histological signs of pancreatitis were observed 48 hr after reperfusion in 80% of the animals treated with melatonin, with only a mild edematous pancreatitis being observed in the remaining rats. Preventive or therapeutic administration of melatonin protected against the induction of oxidative stress and tissue injury, and restored cell function in experimental pancreatic IR in rats.","downloadable_attachments":[{"id":48212173,"asset_id":8108653,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":15733553,"first_name":"CARLOS","last_name":"PERA","domain_name":"independent","page_name":"CARLOSPERA","display_name":"CARLOS PERA","profile_url":"https://independent.academia.edu/CARLOSPERA?f_ri=2256666","photo":"https://0.academia-photos.com/15733553/162642838/152413227/s65_carlos.pera.png"}],"research_interests":[{"id":21143,"name":"Pancreatitis","url":"https://www.academia.edu/Documents/in/Pancreatitis?f_ri=2256666","nofollow":false},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=2256666","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=2256666","nofollow":false},{"id":50841,"name":"Caspases","url":"https://www.academia.edu/Documents/in/Caspases?f_ri=2256666","nofollow":false},{"id":50948,"name":"Ischemia Reperfusion Injury","url":"https://www.academia.edu/Documents/in/Ischemia_Reperfusion_Injury?f_ri=2256666"},{"id":51570,"name":"Melatonin","url":"https://www.academia.edu/Documents/in/Melatonin?f_ri=2256666"},{"id":71400,"name":"Insulin","url":"https://www.academia.edu/Documents/in/Insulin?f_ri=2256666"},{"id":92788,"name":"Pancreas","url":"https://www.academia.edu/Documents/in/Pancreas?f_ri=2256666"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=2256666"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=2256666"},{"id":118450,"name":"Glutathione","url":"https://www.academia.edu/Documents/in/Glutathione?f_ri=2256666"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=2256666"},{"id":141395,"name":"Glutathione Peroxidase","url":"https://www.academia.edu/Documents/in/Glutathione_Peroxidase?f_ri=2256666"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=2256666"},{"id":233372,"name":"Lipid peroxidation","url":"https://www.academia.edu/Documents/in/Lipid_peroxidation?f_ri=2256666"},{"id":247477,"name":"Caspase","url":"https://www.academia.edu/Documents/in/Caspase?f_ri=2256666"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=2256666"},{"id":448603,"name":"Necrosis","url":"https://www.academia.edu/Documents/in/Necrosis?f_ri=2256666"},{"id":564879,"name":"Wistar Rats","url":"https://www.academia.edu/Documents/in/Wistar_Rats?f_ri=2256666"},{"id":576476,"name":"Pineal","url":"https://www.academia.edu/Documents/in/Pineal?f_ri=2256666"},{"id":794984,"name":"Reperfusion injury","url":"https://www.academia.edu/Documents/in/Reperfusion_injury?f_ri=2256666"},{"id":1003462,"name":"Sperm Dna Fragmentation","url":"https://www.academia.edu/Documents/in/Sperm_Dna_Fragmentation?f_ri=2256666"},{"id":1209754,"name":"Lipase","url":"https://www.academia.edu/Documents/in/Lipase?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_10246893" data-work_id="10246893" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/10246893/A_synthetic_DNA_and_fusion_PCR_approach_to_the_ectopic_expression_of_high_levels_of_the_D1_protein_of_photosystem_II_in_Synechocystis_sp_PCC_6803">A synthetic DNA and fusion PCR approach to the ectopic expression of high levels of the D1 protein of photosystem II in Synechocystis sp. PCC 6803</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10246893" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="571f199ea7a6222daa04dcef27eafa5e" rel="nofollow" data-download="{"attachment_id":47469417,"asset_id":10246893,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47469417/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="25082852" href="https://wustl.academia.edu/AparnaNagarajan">Aparna Nagarajan</a><script data-card-contents-for-user="25082852" type="text/json">{"id":25082852,"first_name":"Aparna","last_name":"Nagarajan","domain_name":"wustl","page_name":"AparnaNagarajan","display_name":"Aparna Nagarajan","profile_url":"https://wustl.academia.edu/AparnaNagarajan?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_10246893 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="10246893"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 10246893, container: ".js-paper-rank-work_10246893", }); 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PCC 6803","created_at":"2015-01-20T08:24:08.884-08:00","url":"https://www.academia.edu/10246893/A_synthetic_DNA_and_fusion_PCR_approach_to_the_ectopic_expression_of_high_levels_of_the_D1_protein_of_photosystem_II_in_Synechocystis_sp_PCC_6803?f_ri=2256666","dom_id":"work_10246893","summary":null,"downloadable_attachments":[{"id":47469417,"asset_id":10246893,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":25082852,"first_name":"Aparna","last_name":"Nagarajan","domain_name":"wustl","page_name":"AparnaNagarajan","display_name":"Aparna Nagarajan","profile_url":"https://wustl.academia.edu/AparnaNagarajan?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=2256666","nofollow":false},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=2256666","nofollow":false},{"id":118339,"name":"Polymerase Chain 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itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/26328631/Species_specific_18S_rRNA_gene_amplification_for_the_detection_ofP_falciparumandP_vivaxmalaria_parasites">Species-specific 18S rRNA gene amplification for the detection ofP. falciparumandP. vivaxmalaria parasites</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/26328631" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="06706cef1021576a4f7779c162dc165b" 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{"id":26328631,"title":"Species-specific 18S rRNA gene amplification for the detection ofP. falciparumandP. vivaxmalaria parasites","created_at":"2016-06-20T02:51:40.585-07:00","url":"https://www.academia.edu/26328631/Species_specific_18S_rRNA_gene_amplification_for_the_detection_ofP_falciparumandP_vivaxmalaria_parasites?f_ri=2256666","dom_id":"work_26328631","summary":null,"downloadable_attachments":[{"id":46640234,"asset_id":26328631,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":30606901,"first_name":"Seyed","last_name":"Hasnain","domain_name":"independent","page_name":"SeyedHasnain","display_name":"Seyed Hasnain","profile_url":"https://independent.academia.edu/SeyedHasnain?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=2256666","nofollow":false},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=2256666","nofollow":false},{"id":234147,"name":"ribosomal RNA","url":"https://www.academia.edu/Documents/in/ribosomal_RNA?f_ri=2256666","nofollow":false},{"id":371424,"name":"Genomic DNA","url":"https://www.academia.edu/Documents/in/Genomic_DNA?f_ri=2256666"},{"id":454230,"name":"Plasmodium falciparum","url":"https://www.academia.edu/Documents/in/Plasmodium_falciparum?f_ri=2256666"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity?f_ri=2256666"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=2256666"},{"id":1320136,"name":"Plasmid DNA","url":"https://www.academia.edu/Documents/in/Plasmid_DNA?f_ri=2256666"},{"id":1627985,"name":"Plasmodium Vivax","url":"https://www.academia.edu/Documents/in/Plasmodium_Vivax?f_ri=2256666"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_23975119" 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properties of a novel (–)-hydroxycitric acid extract and its effect on body weight, selected organ weights, hepatic lipid peroxidation and DNA fragmentation, hematology and clinical chemistry, and histopathological changes over a period of 90 days</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/30218197" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4a4c8e633709cb0f2e6c927377182704" rel="nofollow" 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window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=65253066]").text(description); $(".js-view-count-work_65253066").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_65253066").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="65253066"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="62550" href="https://www.academia.edu/Documents/in/Pregnancy">Pregnancy</a>, <script data-card-contents-for-ri="62550" type="text/json">{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a>, <script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="98925" href="https://www.academia.edu/Documents/in/Female">Female</a>, <script data-card-contents-for-ri="98925" type="text/json">{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="98929" href="https://www.academia.edu/Documents/in/Intracytoplasmic_Sperm_Injection">Intracytoplasmic Sperm Injection</a><script data-card-contents-for-ri="98929" type="text/json">{"id":98929,"name":"Intracytoplasmic Sperm Injection","url":"https://www.academia.edu/Documents/in/Intracytoplasmic_Sperm_Injection?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=65253066]'), work: {"id":65253066,"title":"Chromomycin A3 staining, sperm chromatin structure assay and hyaluronic acid binding assay as predictors for assisted reproductive outcome","created_at":"2021-12-21T00:36:33.511-08:00","url":"https://www.academia.edu/65253066/Chromomycin_A3_staining_sperm_chromatin_structure_assay_and_hyaluronic_acid_binding_assay_as_predictors_for_assisted_reproductive_outcome?f_ri=2256666","dom_id":"work_65253066","summary":null,"downloadable_attachments":[{"id":76936529,"asset_id":65253066,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":49372182,"first_name":"Willem","last_name":"Ombelet","domain_name":"independent","page_name":"WillemOmbelet","display_name":"Willem Ombelet","profile_url":"https://independent.academia.edu/WillemOmbelet?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy?f_ri=2256666","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false},{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female?f_ri=2256666","nofollow":false},{"id":98929,"name":"Intracytoplasmic Sperm Injection","url":"https://www.academia.edu/Documents/in/Intracytoplasmic_Sperm_Injection?f_ri=2256666","nofollow":false},{"id":103993,"name":"Assisted Reproduction","url":"https://www.academia.edu/Documents/in/Assisted_Reproduction?f_ri=2256666"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=2256666"},{"id":131185,"name":"Cohort Study","url":"https://www.academia.edu/Documents/in/Cohort_Study?f_ri=2256666"},{"id":141898,"name":"Semen Analysis","url":"https://www.academia.edu/Documents/in/Semen_Analysis?f_ri=2256666"},{"id":238813,"name":"Chromatin","url":"https://www.academia.edu/Documents/in/Chromatin?f_ri=2256666"},{"id":300179,"name":"Hyaluronic Acid","url":"https://www.academia.edu/Documents/in/Hyaluronic_Acid?f_ri=2256666"},{"id":342920,"name":"Fertility Rate","url":"https://www.academia.edu/Documents/in/Fertility_Rate?f_ri=2256666"},{"id":348029,"name":"Fertilization","url":"https://www.academia.edu/Documents/in/Fertilization?f_ri=2256666"},{"id":555922,"name":"Reproductive","url":"https://www.academia.edu/Documents/in/Reproductive?f_ri=2256666"},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=2256666"},{"id":1278603,"name":"Pregnancy Rate","url":"https://www.academia.edu/Documents/in/Pregnancy_Rate?f_ri=2256666"},{"id":1355491,"name":"Pregnancy Outcome","url":"https://www.academia.edu/Documents/in/Pregnancy_Outcome?f_ri=2256666"},{"id":1819400,"name":"Cohort Studies","url":"https://www.academia.edu/Documents/in/Cohort_Studies?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"},{"id":3276666,"name":"fluorescent dyes","url":"https://www.academia.edu/Documents/in/fluorescent_dyes?f_ri=2256666"},{"id":3789883,"name":"Paediatrics and reproductive medicine","url":"https://www.academia.edu/Documents/in/Paediatrics_and_reproductive_medicine?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_5136013" data-work_id="5136013" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/5136013/Polymorphism_analysis_of_housekeeping_genes_for_identification_and_differentiation_of_Clavibacter_michiganensis_subspecies">Polymorphism analysis of housekeeping genes for identification and differentiation of Clavibacter michiganensis subspecies</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The utility of polymorphism analysis was determined for differentiation of the following subspecies of the Gram-positive plant pathogenic bacterium, Clavibacter michiganensis: C. m. subsp. michiganensis, C. m. subsp. sepedonicus, C. m.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_5136013" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The utility of polymorphism analysis was determined for differentiation of the following subspecies of the Gram-positive plant pathogenic bacterium, Clavibacter michiganensis: C. m. subsp. michiganensis, C. m. subsp. sepedonicus, C. m. subsp. insidiosus C. m. subsp. nebraskensis, and C. m. subsp. tessellarius. Specific primers designed for amplification of the housekeeping genes recA, rpoB, and rpoD generated 827-, 1037-, and 862-bp DNA fragments, respectively. PCR products obtained from 40 C. michiganensis strains were analysed using RFLP with four restriction endonucleases, and those PCR products with specific RFLP patterns were sequenced. The genotypes discriminated after PCR–RFLP were specific for each subspecies and also allowed for differentiation of C. m. subsp. michiganensis strains. Sequence analysis of the recA, rpoB, and rpoD gene fragments also distinguished C. michiganensis subspecies and was useful for phylogenetic analysis of all subspecies. For rapid, inexpensive, and effective differentiation of the five subspecies in this research, we recommend the amplification of recA and/or rpoD gene fragments and digestion of the PCR products with the restriction endonuclease FnuDII.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/5136013" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a96bdfcfd2d2c10362133ad743a383dc" rel="nofollow" data-download="{"attachment_id":49435765,"asset_id":5136013,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49435765/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="6922053" href="https://poznan.academia.edu/JoannaKamasa">Joanna Kamasa</a><script data-card-contents-for-user="6922053" type="text/json">{"id":6922053,"first_name":"Joanna","last_name":"Kamasa","domain_name":"poznan","page_name":"JoannaKamasa","display_name":"Joanna Kamasa","profile_url":"https://poznan.academia.edu/JoannaKamasa?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_5136013 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="5136013"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 5136013, container: ".js-paper-rank-work_5136013", }); 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$(".js-view-count[data-work-id=5136013]").text(description); $(".js-view-count-work_5136013").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_5136013").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="5136013"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="3855" href="https://www.academia.edu/Documents/in/Polymorphism">Polymorphism</a>, <script data-card-contents-for-ri="3855" type="text/json">{"id":3855,"name":"Polymorphism","url":"https://www.academia.edu/Documents/in/Polymorphism?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="29082" href="https://www.academia.edu/Documents/in/Sequence_Analysis">Sequence Analysis</a><script data-card-contents-for-ri="29082" type="text/json">{"id":29082,"name":"Sequence Analysis","url":"https://www.academia.edu/Documents/in/Sequence_Analysis?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=5136013]'), work: {"id":5136013,"title":"Polymorphism analysis of housekeeping genes for identification and differentiation of Clavibacter michiganensis subspecies","created_at":"2013-11-17T22:00:07.638-08:00","url":"https://www.academia.edu/5136013/Polymorphism_analysis_of_housekeeping_genes_for_identification_and_differentiation_of_Clavibacter_michiganensis_subspecies?f_ri=2256666","dom_id":"work_5136013","summary":"The utility of polymorphism analysis was determined for differentiation of the following subspecies of the Gram-positive plant pathogenic bacterium, Clavibacter michiganensis: C. m. subsp. michiganensis, C. m. subsp. sepedonicus, C. m. subsp. insidiosus C. m. subsp. nebraskensis, and C. m. subsp. tessellarius. Specific primers designed for amplification of the housekeeping genes recA, rpoB, and rpoD generated 827-, 1037-, and 862-bp DNA fragments, respectively. PCR products obtained from 40 C. michiganensis strains were analysed using RFLP with four restriction endonucleases, and those PCR products with specific RFLP patterns were sequenced. The genotypes discriminated after PCR–RFLP were specific for each subspecies and also allowed for differentiation of C. m. subsp. michiganensis strains. Sequence analysis of the recA, rpoB, and rpoD gene fragments also distinguished C. michiganensis subspecies and was useful for phylogenetic analysis of all subspecies. For rapid, inexpensive, and effective differentiation of the five subspecies in this research, we recommend the amplification of recA and/or rpoD gene fragments and digestion of the PCR products with the restriction endonuclease FnuDII.","downloadable_attachments":[{"id":49435765,"asset_id":5136013,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":6922053,"first_name":"Joanna","last_name":"Kamasa","domain_name":"poznan","page_name":"JoannaKamasa","display_name":"Joanna Kamasa","profile_url":"https://poznan.academia.edu/JoannaKamasa?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=2256666","nofollow":false},{"id":3855,"name":"Polymorphism","url":"https://www.academia.edu/Documents/in/Polymorphism?f_ri=2256666","nofollow":false},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=2256666","nofollow":false},{"id":29082,"name":"Sequence Analysis","url":"https://www.academia.edu/Documents/in/Sequence_Analysis?f_ri=2256666","nofollow":false},{"id":83825,"name":"Gram Positive","url":"https://www.academia.edu/Documents/in/Gram_Positive?f_ri=2256666"},{"id":115599,"name":"Primer design","url":"https://www.academia.edu/Documents/in/Primer_design?f_ri=2256666"},{"id":158782,"name":"Phylogenetic analysis","url":"https://www.academia.edu/Documents/in/Phylogenetic_analysis?f_ri=2256666"},{"id":607003,"name":"Plant Pathogen","url":"https://www.academia.edu/Documents/in/Plant_Pathogen?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9584013" data-work_id="9584013" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9584013/Multiple_sclerosis_Oligodendrocytes_display_cell_death_related_molecules_in_situ_but_do_not_undergo_apoptosis">Multiple sclerosis: Oligodendrocytes display cell death-related molecules in situ but do not undergo apoptosis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To investigate whether apoptosis is involved in the fate of oligodendrocytes in chronic multiple sclerosis lesions, the pro-apoptotic molecules fas and tumor necrosis factor receptors and the anti-apoptotic molecule bcl-2 were examined by... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_9584013" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To investigate whether apoptosis is involved in the fate of oligodendrocytes in chronic multiple sclerosis lesions, the pro-apoptotic molecules fas and tumor necrosis factor receptors and the anti-apoptotic molecule bcl-2 were examined by immunohistochemistry, and DNA fragmentation was assessed by an end labeling technique. Fas and both tumor necrosis factor receptors were preferentially expressed on oligodendrocytes in multiple sclerosis lesions, this phenotype being more evident at the lesion edge. The ligand for fasL, was constitutively present at high levels on microglia. The anti-apoptotic molecule bcl-2 was selectively expressed on oligodendrocytes in silent lesions and on astrocytes in active lesions. These molecules were also detected in control material, albeit at lower levels. In chronic active lesions, a few inflammatory cells displayed fas reactivity, whereas the majority expressed bcl-2. DNA fragmentation was found in a number of infiltrating cells and some microglia, whereas, with one possible exception, oligodendrocytes showed no evidence of apoptosis. Thus, while apoptosis is involved in the elimination of infiltrating cells, it plays little or no role in oligodendrocyte depletion in multiple sclerosis, a process that may be related to a lytic pathway. In addition, microglia constitutively displayed the ligand for fas, and appeared to be the major effector cell population in the central nervous system.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9584013" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ce59676239c6c0651bd10ee5a227bd7e" rel="nofollow" data-download="{"attachment_id":47716335,"asset_id":9584013,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47716335/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="22692058" href="https://asem.academia.edu/BrunoBonetti">Bruno Bonetti</a><script data-card-contents-for-user="22692058" type="text/json">{"id":22692058,"first_name":"Bruno","last_name":"Bonetti","domain_name":"asem","page_name":"BrunoBonetti","display_name":"Bruno Bonetti","profile_url":"https://asem.academia.edu/BrunoBonetti?f_ri=2256666","photo":"https://0.academia-photos.com/22692058/8363502/9352294/s65_bruno.bonetti.jpg_oh_752a70e79e88300a05773b7c75bb3700_oe_55b23e35___gda___1437955274_87b658fd40900f4460e5d404b5f3000c"}</script></span></span></li><li class="js-paper-rank-work_9584013 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="9584013"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 9584013, container: ".js-paper-rank-work_9584013", }); 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$(".js-view-count[data-work-id=9584013]").text(description); $(".js-view-count-work_9584013").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_9584013").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="9584013"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3097" href="https://www.academia.edu/Documents/in/Multiple_sclerosis">Multiple sclerosis</a>, <script data-card-contents-for-ri="3097" type="text/json">{"id":3097,"name":"Multiple sclerosis","url":"https://www.academia.edu/Documents/in/Multiple_sclerosis?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="24731" href="https://www.academia.edu/Documents/in/Apoptosis">Apoptosis</a>, <script data-card-contents-for-ri="24731" type="text/json">{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a><script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=9584013]'), work: {"id":9584013,"title":"Multiple sclerosis: Oligodendrocytes display cell death-related molecules in situ but do not undergo apoptosis","created_at":"2014-12-01T17:48:39.862-08:00","url":"https://www.academia.edu/9584013/Multiple_sclerosis_Oligodendrocytes_display_cell_death_related_molecules_in_situ_but_do_not_undergo_apoptosis?f_ri=2256666","dom_id":"work_9584013","summary":"To investigate whether apoptosis is involved in the fate of oligodendrocytes in chronic multiple sclerosis lesions, the pro-apoptotic molecules fas and tumor necrosis factor receptors and the anti-apoptotic molecule bcl-2 were examined by immunohistochemistry, and DNA fragmentation was assessed by an end labeling technique. Fas and both tumor necrosis factor receptors were preferentially expressed on oligodendrocytes in multiple sclerosis lesions, this phenotype being more evident at the lesion edge. The ligand for fasL, was constitutively present at high levels on microglia. The anti-apoptotic molecule bcl-2 was selectively expressed on oligodendrocytes in silent lesions and on astrocytes in active lesions. These molecules were also detected in control material, albeit at lower levels. In chronic active lesions, a few inflammatory cells displayed fas reactivity, whereas the majority expressed bcl-2. DNA fragmentation was found in a number of infiltrating cells and some microglia, whereas, with one possible exception, oligodendrocytes showed no evidence of apoptosis. Thus, while apoptosis is involved in the elimination of infiltrating cells, it plays little or no role in oligodendrocyte depletion in multiple sclerosis, a process that may be related to a lytic pathway. In addition, microglia constitutively displayed the ligand for fas, and appeared to be the major effector cell population in the central nervous system.","downloadable_attachments":[{"id":47716335,"asset_id":9584013,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":22692058,"first_name":"Bruno","last_name":"Bonetti","domain_name":"asem","page_name":"BrunoBonetti","display_name":"Bruno Bonetti","profile_url":"https://asem.academia.edu/BrunoBonetti?f_ri=2256666","photo":"https://0.academia-photos.com/22692058/8363502/9352294/s65_bruno.bonetti.jpg_oh_752a70e79e88300a05773b7c75bb3700_oe_55b23e35___gda___1437955274_87b658fd40900f4460e5d404b5f3000c"}],"research_interests":[{"id":3097,"name":"Multiple sclerosis","url":"https://www.academia.edu/Documents/in/Multiple_sclerosis?f_ri=2256666","nofollow":false},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=2256666","nofollow":false},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=2256666","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false},{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female?f_ri=2256666"},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death?f_ri=2256666"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=2256666"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=2256666"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=2256666"},{"id":295155,"name":"Middle Aged","url":"https://www.academia.edu/Documents/in/Middle_Aged?f_ri=2256666"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult?f_ri=2256666"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_12604644" data-work_id="12604644" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/12604644/Detection_of_histamine_producing_bacteria_using_polymerase_chain_reaction_techniques_and_DNA_probes">Detection of histamine-producing bacteria using polymerase chain reaction techniques and DNA probes</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/12604644" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bdbd999b5f8a9108be8a845caa61e470" rel="nofollow" data-download="{"attachment_id":46055800,"asset_id":12604644,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46055800/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="31548307" href="https://independent.academia.edu/MariaLiduinaAlvesMartins">Maria Liduina Alves Martins</a><script data-card-contents-for-user="31548307" type="text/json">{"id":31548307,"first_name":"Maria Liduina","last_name":"Alves Martins","domain_name":"independent","page_name":"MariaLiduinaAlvesMartins","display_name":"Maria Liduina Alves Martins","profile_url":"https://independent.academia.edu/MariaLiduinaAlvesMartins?f_ri=2256666","photo":"https://0.academia-photos.com/31548307/9327908/10396210/s65_maria_liduina.alves_martins.jpg_oh_44d6a72ec84c88610e51276042a4ddfe_oe_55f2a808___gda___1441944581_b312a6fce67206843519ac976b840d8a"}</script></span></span></li><li class="js-paper-rank-work_12604644 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="12604644"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 12604644, container: ".js-paper-rank-work_12604644", }); });</script></li><li class="js-percentile-work_12604644 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 12604644; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_12604644"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_12604644 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="12604644"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 12604644; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=12604644]").text(description); $(".js-view-count-work_12604644").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_12604644").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="12604644"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="14907" href="https://www.academia.edu/Documents/in/Industrial_Biotechnology">Industrial Biotechnology</a>, <script data-card-contents-for-ri="14907" type="text/json">{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="118339" href="https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction">Polymerase Chain Reaction</a>, <script data-card-contents-for-ri="118339" type="text/json">{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="573653" href="https://www.academia.edu/Documents/in/Food_Sciences">Food Sciences</a>, <script data-card-contents-for-ri="573653" type="text/json">{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1146746" href="https://www.academia.edu/Documents/in/Clostridium_perfringens">Clostridium perfringens</a><script data-card-contents-for-ri="1146746" type="text/json">{"id":1146746,"name":"Clostridium perfringens","url":"https://www.academia.edu/Documents/in/Clostridium_perfringens?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=12604644]'), work: {"id":12604644,"title":"Detection of histamine-producing bacteria using polymerase chain reaction techniques and DNA probes","created_at":"2015-05-26T05:22:17.815-07:00","url":"https://www.academia.edu/12604644/Detection_of_histamine_producing_bacteria_using_polymerase_chain_reaction_techniques_and_DNA_probes?f_ri=2256666","dom_id":"work_12604644","summary":null,"downloadable_attachments":[{"id":46055800,"asset_id":12604644,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":31548307,"first_name":"Maria Liduina","last_name":"Alves Martins","domain_name":"independent","page_name":"MariaLiduinaAlvesMartins","display_name":"Maria Liduina Alves Martins","profile_url":"https://independent.academia.edu/MariaLiduinaAlvesMartins?f_ri=2256666","photo":"https://0.academia-photos.com/31548307/9327908/10396210/s65_maria_liduina.alves_martins.jpg_oh_44d6a72ec84c88610e51276042a4ddfe_oe_55f2a808___gda___1441944581_b312a6fce67206843519ac976b840d8a"}],"research_interests":[{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=2256666","nofollow":false},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=2256666","nofollow":false},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=2256666","nofollow":false},{"id":1146746,"name":"Clostridium perfringens","url":"https://www.academia.edu/Documents/in/Clostridium_perfringens?f_ri=2256666","nofollow":false},{"id":1237507,"name":"European Food","url":"https://www.academia.edu/Documents/in/European_Food?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13574104 coauthored" data-work_id="13574104" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13574104/New_Method_for_Simultaneous_Species_Specific_Identification_of_Equine_Strongyles_Nematoda_Strongylida_by_Reverse_Line_Blot_Hybridization">New Method for Simultaneous Species-Specific Identification of Equine Strongyles (Nematoda, Strongylida) by Reverse Line Blot Hybridization</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The ability of a reverse line blot (RLB) assay to identify 13 common species of equine small strongyles (cyathostomins) and to discriminate them from three Strongylus spp. (large strongyles) was demonstrated. The assay relied on the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13574104" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The ability of a reverse line blot (RLB) assay to identify 13 common species of equine small strongyles (cyathostomins) and to discriminate them from three Strongylus spp. (large strongyles) was demonstrated. The assay relied on the specific hybridization of PCR-amplified intergenic spacer DNA fragments of the nuclear ribosomal DNA to membrane-bound species-specific probes. All cyathostomins examined were unequivocally identified and</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13574104" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="086be1011c4781517a7288b4e79b8a05" rel="nofollow" data-download="{"attachment_id":38078168,"asset_id":13574104,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/38078168/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32756279" href="https://independent.academia.edu/RaffaellaIorio">Raffaella Iorio</a><script data-card-contents-for-user="32756279" type="text/json">{"id":32756279,"first_name":"Raffaella","last_name":"Iorio","domain_name":"independent","page_name":"RaffaellaIorio","display_name":"Raffaella Iorio","profile_url":"https://independent.academia.edu/RaffaellaIorio?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13574104">+1</span><div class="hidden js-additional-users-13574104"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://izan-kiev.academia.edu/VitaliyKharchenko">Vitaliy Kharchenko</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13574104'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13574104').html(); 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The assay relied on the specific hybridization of PCR-amplified intergenic spacer DNA fragments of the nuclear ribosomal DNA to membrane-bound species-specific probes. All cyathostomins examined were unequivocally identified and","downloadable_attachments":[{"id":38078168,"asset_id":13574104,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32756279,"first_name":"Raffaella","last_name":"Iorio","domain_name":"independent","page_name":"RaffaellaIorio","display_name":"Raffaella Iorio","profile_url":"https://independent.academia.edu/RaffaellaIorio?f_ri=2256666","photo":"/images/s65_no_pic.png"},{"id":32836161,"first_name":"Vitaliy","last_name":"Kharchenko","domain_name":"izan-kiev","page_name":"VitaliyKharchenko","display_name":"Vitaliy Kharchenko","profile_url":"https://izan-kiev.academia.edu/VitaliyKharchenko?f_ri=2256666","photo":"https://0.academia-photos.com/32836161/15389689/16025968/s65_vitaliy.kharchenko.jpg"}],"research_interests":[{"id":43666,"name":"Clinical Microbiology","url":"https://www.academia.edu/Documents/in/Clinical_Microbiology?f_ri=2256666","nofollow":false},{"id":47884,"name":"Biological 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Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=2256666"},{"id":1623426,"name":"Strongylida","url":"https://www.academia.edu/Documents/in/Strongylida?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15509774" data-work_id="15509774" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15509774/Chlorophyllin_protects_HEp_2_cells_from_nuclear_fragmentation_induced_by_poliovirus">Chlorophyllin protects HEp-2 cells from nuclear fragmentation induced by poliovirus</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15509774" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6c709aacd743bdc440a50ec8e26c8239" rel="nofollow" data-download="{"attachment_id":38706346,"asset_id":15509774,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/38706346/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" 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class="js-view-count view-count u-mr2x" data-work-id="15509774"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 15509774; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=15509774]").text(description); $(".js-view-count-work_15509774").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_15509774").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="15509774"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="28235" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="48414" href="https://www.academia.edu/Documents/in/Applied_Economics_Letters">Applied Economics Letters</a>, <script data-card-contents-for-ri="48414" type="text/json">{"id":48414,"name":"Applied Economics Letters","url":"https://www.academia.edu/Documents/in/Applied_Economics_Letters?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a>, <script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false}</script><a 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poliovirus","created_at":"2015-09-08T07:19:56.173-07:00","url":"https://www.academia.edu/15509774/Chlorophyllin_protects_HEp_2_cells_from_nuclear_fragmentation_induced_by_poliovirus?f_ri=2256666","dom_id":"work_15509774","summary":null,"downloadable_attachments":[{"id":38706346,"asset_id":15509774,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34660715,"first_name":"Mário","last_name":"Mantovani","domain_name":"independent","page_name":"MárioMantovani","display_name":"Mário Mantovani","profile_url":"https://independent.academia.edu/M%C3%A1rioMantovani?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=2256666","nofollow":false},{"id":48414,"name":"Applied Economics Letters","url":"https://www.academia.edu/Documents/in/Applied_Economics_Letters?f_ri=2256666","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666","nofollow":false},{"id":279382,"name":"Comet Assay","url":"https://www.academia.edu/Documents/in/Comet_Assay?f_ri=2256666","nofollow":false},{"id":317801,"name":"Cell nucleus","url":"https://www.academia.edu/Documents/in/Cell_nucleus?f_ri=2256666"},{"id":1622318,"name":"Antiviral Agents","url":"https://www.academia.edu/Documents/in/Antiviral_Agents?f_ri=2256666"},{"id":1888098,"name":"Poliovirus","url":"https://www.academia.edu/Documents/in/Poliovirus?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_5448076" data-work_id="5448076" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/5448076/Development_of_Yellow_Mosaic_Virus_YMV_resistance_linked_DNA_marker_in_Vigna_mungo_from_populations_segregating_for_YMV_reaction">Development of Yellow Mosaic Virus (YMV) resistance linked DNA marker in Vigna mungo from populations segregating for YMV-reaction</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Yellow mosaic virus, YMV, causes one of the most severe of biotic stresses in Vignas, an important group of pulse crops. The viral disease is transmitted through the white fly, Bemicia tabaci, and the yield of the plants is affected... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_5448076" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Yellow mosaic virus, YMV, causes one of the most severe of biotic stresses in Vignas, an important group of pulse crops. The viral disease is transmitted through the white fly, Bemicia tabaci, and the yield of the plants is affected drastically. YMV-tolerant lines, generated from a single YMV-tolerant plant identified in the field within a large population of the susceptible cultivar T-9, were crossed with T-9, and F1, F2 and F3 progenies raised. The different generations were phenotyped for YMV-reaction by forced inoculation using viruliferous white flies. A monogenic recessive control of YMV-tolerance was revealed from the F2 segregation ratio of 3:1 (susceptible: tolerant), which was confirmed by the segregation ratio of the F3 families. Of 24 pairs of resistance gene analog (RGA) primers screened, only one pair, RGA 1F-CG/RGA 1R, was found to be polymorphic among the parents. Selected F2 individuals and F3 families were genotyped with the polymorphic RGA primer pair and the polymorphism was found to be linked with YMV-reaction. This primer pair amplified a 445bp DNA fragment only from homozygous tolerant and the heterozygous lines. The 445bp marker band was sequenced and named ‘VMYR1’. The predicted amino acid sequence showed highly significant homology with the NB-ARC domain present in several gene products involved in plant disease resistance, nematode cell death and human apoptotic signaling. To the best of our knowledge, this is the first report of YMV-resistance linked DNA marker development in any crop species using segregating populations. This YMV-resistance linked marker is of potential commercial importance in resistance breeding of plants.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/5448076" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="463af4e83435aece856ac13ccf3b9986" rel="nofollow" data-download="{"attachment_id":32571352,"asset_id":5448076,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/32571352/download_file?st=MTczMjM3NDI2OSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="7635053" href="https://visva-bharati.academia.edu/JollyBasak">Jolly Basak</a><script data-card-contents-for-user="7635053" type="text/json">{"id":7635053,"first_name":"Jolly","last_name":"Basak","domain_name":"visva-bharati","page_name":"JollyBasak","display_name":"Jolly Basak","profile_url":"https://visva-bharati.academia.edu/JollyBasak?f_ri=2256666","photo":"https://0.academia-photos.com/7635053/49942674/37934263/s65_jolly.basak.jpeg"}</script></span></span></li><li class="js-paper-rank-work_5448076 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="5448076"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 5448076, container: ".js-paper-rank-work_5448076", }); 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The viral disease is transmitted through the white fly, Bemicia tabaci, and the yield of the plants is affected drastically. YMV-tolerant lines, generated from a single YMV-tolerant plant identified in the field within a large population of the susceptible cultivar T-9, were crossed with T-9, and F1, F2 and F3 progenies raised. The different generations were phenotyped for YMV-reaction by forced inoculation using viruliferous white flies. A monogenic recessive control of YMV-tolerance was revealed from the F2 segregation ratio of 3:1 (susceptible: tolerant), which was confirmed by the segregation ratio of the F3 families. Of 24 pairs of resistance gene analog (RGA) primers screened, only one pair, RGA 1F-CG/RGA 1R, was found to be polymorphic among the parents. Selected F2 individuals and F3 families were genotyped with the polymorphic RGA primer pair and the polymorphism was found to be linked with YMV-reaction. This primer pair amplified a 445bp DNA fragment only from homozygous tolerant and the heterozygous lines. The 445bp marker band was sequenced and named ‘VMYR1’. The predicted amino acid sequence showed highly significant homology with the NB-ARC domain present in several gene products involved in plant disease resistance, nematode cell death and human apoptotic signaling. To the best of our knowledge, this is the first report of YMV-resistance linked DNA marker development in any crop species using segregating populations. This YMV-resistance linked marker is of potential commercial importance in resistance breeding of plants.","downloadable_attachments":[{"id":32571352,"asset_id":5448076,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":7635053,"first_name":"Jolly","last_name":"Basak","domain_name":"visva-bharati","page_name":"JollyBasak","display_name":"Jolly Basak","profile_url":"https://visva-bharati.academia.edu/JollyBasak?f_ri=2256666","photo":"https://0.academia-photos.com/7635053/49942674/37934263/s65_jolly.basak.jpeg"}],"research_interests":[{"id":3855,"name":"Polymorphism","url":"https://www.academia.edu/Documents/in/Polymorphism?f_ri=2256666","nofollow":false},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=2256666","nofollow":false},{"id":14938,"name":"Molecular Breeding","url":"https://www.academia.edu/Documents/in/Molecular_Breeding?f_ri=2256666","nofollow":false},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death?f_ri=2256666","nofollow":false},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=2256666"},{"id":1172657,"name":"Plant Disease Resistance","url":"https://www.academia.edu/Documents/in/Plant_Disease_Resistance?f_ri=2256666"},{"id":1532565,"name":"DNA marker","url":"https://www.academia.edu/Documents/in/DNA_marker?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_26207722 coauthored" data-work_id="26207722" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" 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Applications","created_at":"2016-06-15T23:46:46.598-07:00","url":"https://www.academia.edu/26207722/An_Efficient_Multistrategy_DNA_Decontamination_Procedure_of_PCR_Reagents_for_Hypersensitive_PCR_Applications?f_ri=2256666","dom_id":"work_26207722","summary":null,"downloadable_attachments":[{"id":46528726,"asset_id":26207722,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":1754245,"first_name":"Melanie","last_name":"Pruvost","domain_name":"ijm","page_name":"MelaniePruvost","display_name":"Melanie Pruvost","profile_url":"https://ijm.academia.edu/MelaniePruvost?f_ri=2256666","photo":"https://0.academia-photos.com/1754245/601664/747281/s65_melanie.pruvost.jpg"},{"id":50116578,"first_name":"Thierry","last_name":"Grange","domain_name":"independent","page_name":"TGrange","display_name":"Thierry Grange","profile_url":"https://independent.academia.edu/TGrange?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=2256666","nofollow":false},{"id":498,"name":"Physics","url":"https://www.academia.edu/Documents/in/Physics?f_ri=2256666","nofollow":false},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=2256666","nofollow":false},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology?f_ri=2256666","nofollow":false},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=2256666"},{"id":22998,"name":"Gamma 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itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14475214/Dill_Anethum_graveolens_L_seed_essential_oil_induces_Candida_albicans_apoptosis_in_a_metacaspase_dependent_manner">Dill (Anethum graveolens L.) seed essential oil induces Candida albicans apoptosis in a metacaspase-dependent manner</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Dill (Anethum graveolens L.) has been used in traditional Uighur medicine for its various pharmacological activities. Previous studies have suggested that dill seed essential oil (DSEO) has anti-Candida potential and the mechanism of its... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14475214" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Dill (Anethum graveolens L.) has been used in traditional Uighur medicine for its various pharmacological activities. Previous studies have suggested that dill seed essential oil (DSEO) has anti-Candida potential and the mechanism of its action also has been studied. Our study examined whether DSEO induces apoptosis in the human pathogen Candida albicans ATCC 64550. Our results indicate that C. albicans ATCC 64550 cells treated with DSEO show some typical apoptosis characters, such as decrease in adenosine triphosphatase (ATPase) activity, chromatin condensation, nuclear fragmentation, and phosphatidylserine (PS) exposure. The DSEO promoted cytochrome c (cyt c) release and metacaspase activation, which resulted in C. albicans ATCC 64550 apoptosis. L-cysteine prevented the DSEO-induced nuclear fragmentation, PS externalization, and metacaspase activation, thus indicating that the reactive oxygen species (ROS) is an important mediator of DSEO-induced apoptosis. To our knowledge, this ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14475214" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="06580dd08ad53928d1b12ba79087ea73" rel="nofollow" data-download="{"attachment_id":44124686,"asset_id":14475214,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44124686/download_file?st=MTczMjM3NDI3MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33521728" href="https://independent.academia.edu/HongZeng">Hong Zeng</a><script data-card-contents-for-user="33521728" type="text/json">{"id":33521728,"first_name":"Hong","last_name":"Zeng","domain_name":"independent","page_name":"HongZeng","display_name":"Hong Zeng","profile_url":"https://independent.academia.edu/HongZeng?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-14475214">+1</span><div class="hidden js-additional-users-14475214"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/YuxinChen5">Yuxin Chen</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-14475214'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-14475214').html(); 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Previous studies have suggested that dill seed essential oil (DSEO) has anti-Candida potential and the mechanism of its action also has been studied. Our study examined whether DSEO induces apoptosis in the human pathogen Candida albicans ATCC 64550. Our results indicate that C. albicans ATCC 64550 cells treated with DSEO show some typical apoptosis characters, such as decrease in adenosine triphosphatase (ATPase) activity, chromatin condensation, nuclear fragmentation, and phosphatidylserine (PS) exposure. The DSEO promoted cytochrome c (cyt c) release and metacaspase activation, which resulted in C. albicans ATCC 64550 apoptosis. L-cysteine prevented the DSEO-induced nuclear fragmentation, PS externalization, and metacaspase activation, thus indicating that the reactive oxygen species (ROS) is an important mediator of DSEO-induced apoptosis. To our knowledge, this ...","downloadable_attachments":[{"id":44124686,"asset_id":14475214,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33521728,"first_name":"Hong","last_name":"Zeng","domain_name":"independent","page_name":"HongZeng","display_name":"Hong Zeng","profile_url":"https://independent.academia.edu/HongZeng?f_ri=2256666","photo":"/images/s65_no_pic.png"},{"id":33416164,"first_name":"Yuxin","last_name":"Chen","domain_name":"independent","page_name":"YuxinChen5","display_name":"Yuxin Chen","profile_url":"https://independent.academia.edu/YuxinChen5?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=2256666","nofollow":false},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=2256666","nofollow":false},{"id":24731,"name":"Apoptosis","url":"https://www.academia.edu/Documents/in/Apoptosis?f_ri=2256666","nofollow":false},{"id":50841,"name":"Caspases","url":"https://www.academia.edu/Documents/in/Caspases?f_ri=2256666","nofollow":false},{"id":82978,"name":"Reactive Oxygen Species","url":"https://www.academia.edu/Documents/in/Reactive_Oxygen_Species?f_ri=2256666"},{"id":131573,"name":"Candida albicans","url":"https://www.academia.edu/Documents/in/Candida_albicans?f_ri=2256666"},{"id":357551,"name":"Fungal Biology","url":"https://www.academia.edu/Documents/in/Fungal_Biology?f_ri=2256666"},{"id":386438,"name":"Seeds","url":"https://www.academia.edu/Documents/in/Seeds?f_ri=2256666"},{"id":552209,"name":"Antifungal Agents","url":"https://www.academia.edu/Documents/in/Antifungal_Agents?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21791676 coauthored" data-work_id="21791676" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21791676/T_cell_receptor_excision_circles_as_markers_for_recent_thymic_emigrants_basic_aspects_technical_approach_and_guidelines_for_interpretation">T cell receptor excision circles as markers for recent thymic emigrants: basic aspects, technical approach, and guidelines for interpretation</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21791676" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="fb69595e1a60c831195e0914cfd1ec1e" rel="nofollow" data-download="{"attachment_id":42555243,"asset_id":21791676,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42555243/download_file?st=MTczMjM3NDI3MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="54796049" href="https://independent.academia.edu/D%C3%B6rteHamann">Dörte Hamann</a><script data-card-contents-for-user="54796049" type="text/json">{"id":54796049,"first_name":"Dörte","last_name":"Hamann","domain_name":"independent","page_name":"DörteHamann","display_name":"Dörte Hamann","profile_url":"https://independent.academia.edu/D%C3%B6rteHamann?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-21791676">+2</span><div class="hidden js-additional-users-21791676"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/MetteHazenberg">Mette Hazenberg</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/VerschurenM">M. Verschuren</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-21791676'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-21791676').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_21791676 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21791676"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21791676, container: ".js-paper-rank-work_21791676", }); });</script></li><li class="js-percentile-work_21791676 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21791676; 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Verschuren","profile_url":"https://independent.academia.edu/VerschurenM?f_ri=2256666","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5072,"name":"Molecular Medicine","url":"https://www.academia.edu/Documents/in/Molecular_Medicine?f_ri=2256666","nofollow":false},{"id":34021,"name":"T cell receptor","url":"https://www.academia.edu/Documents/in/T_cell_receptor?f_ri=2256666","nofollow":false},{"id":38650,"name":"Cell Division","url":"https://www.academia.edu/Documents/in/Cell_Division?f_ri=2256666","nofollow":false},{"id":39978,"name":"HIV","url":"https://www.academia.edu/Documents/in/HIV?f_ri=2256666","nofollow":false},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation?f_ri=2256666"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=2256666"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=2256666"},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death?f_ri=2256666"},{"id":280237,"name":"T lymphocytes","url":"https://www.academia.edu/Documents/in/T_lymphocytes?f_ri=2256666"},{"id":318308,"name":"Human immunodeficiency virus","url":"https://www.academia.edu/Documents/in/Human_immunodeficiency_virus?f_ri=2256666"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=2256666"},{"id":568482,"name":"Biological markers","url":"https://www.academia.edu/Documents/in/Biological_markers?f_ri=2256666"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_80717667" data-work_id="80717667" 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data-card-contents-for-ri="2215" type="text/json">{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9991" href="https://www.academia.edu/Documents/in/Wastewater_Treatment">Wastewater Treatment</a>, <script data-card-contents-for-ri="9991" type="text/json">{"id":9991,"name":"Wastewater Treatment","url":"https://www.academia.edu/Documents/in/Wastewater_Treatment?f_ri=2256666","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="11417" href="https://www.academia.edu/Documents/in/Population_Dynamics">Population Dynamics</a><script data-card-contents-for-ri="11417" type="text/json">{"id":11417,"name":"Population Dynamics","url":"https://www.academia.edu/Documents/in/Population_Dynamics?f_ri=2256666","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: 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class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/69708934/Development_of_a_molecular_marker_for_specific_detection_of_Fusarium_oxysporum_f_sp_cubense_race_4">Development of a molecular marker for specific detection of Fusarium oxysporum f. sp. cubense race 4</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Fusarium oxysporum f. sp. cubense is the causal agent of Panama disease of banana. A rapid and reliable diagnosis is the foundation of integrated disease management practices in commodity crops. For this diagnostic purpose, we have... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_69708934" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Fusarium oxysporum f. sp. cubense is the causal agent of Panama disease of banana. A rapid and reliable diagnosis is the foundation of integrated disease management practices in commodity crops. For this diagnostic purpose, we have developed a reliable molecular method to detect Foc race 4 isolates in Taiwan. By PCR amplification, the primer set Foc-1/Foc-2 derived from the sequence</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/69708934" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="93ae91905e5a15571a18d7a23f642fe4" rel="nofollow" data-download="{"attachment_id":79700883,"asset_id":69708934,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/79700883/download_file?st=MTczMjM3NDI3MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41006760" href="https://independent.academia.edu/YingHongLin">Ying-Hong Lin</a><script data-card-contents-for-user="41006760" type="text/json">{"id":41006760,"first_name":"Ying-Hong","last_name":"Lin","domain_name":"independent","page_name":"YingHongLin","display_name":"Ying-Hong Lin","profile_url":"https://independent.academia.edu/YingHongLin?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_69708934 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="69708934"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 69708934, container: ".js-paper-rank-work_69708934", }); 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u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13602246" data-work_id="13602246" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13602246/Telomere_length_predicts_embryo_fragmentation_after_in_vitro_fertilization_in_women_Toward_a_telomere_theory_of_reproductive_aging_in_women">Telomere length predicts embryo fragmentation after in vitro fertilization in women—Toward a telomere theory of reproductive aging in women</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13602246" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa 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itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32777346" href="https://nyu.academia.edu/DavidKeefe">David Keefe</a><script data-card-contents-for-user="32777346" type="text/json">{"id":32777346,"first_name":"David","last_name":"Keefe","domain_name":"nyu","page_name":"DavidKeefe","display_name":"David Keefe","profile_url":"https://nyu.academia.edu/DavidKeefe?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_13602246 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13602246"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13602246, container: ".js-paper-rank-work_13602246", }); });</script></li><li class="js-percentile-work_13602246 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span 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LENGTH","url":"https://www.academia.edu/Documents/in/TELOMERE_LENGTH?f_ri=2256666"},{"id":210533,"name":"Embryo Transfer","url":"https://www.academia.edu/Documents/in/Embryo_Transfer?f_ri=2256666"},{"id":217865,"name":"Reproductive medicine","url":"https://www.academia.edu/Documents/in/Reproductive_medicine?f_ri=2256666"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult?f_ri=2256666"},{"id":613442,"name":"Fertilization in Vitro","url":"https://www.academia.edu/Documents/in/Fertilization_in_Vitro?f_ri=2256666"},{"id":693013,"name":"Telomere","url":"https://www.academia.edu/Documents/in/Telomere?f_ri=2256666"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=2256666"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=2256666"},{"id":1027717,"name":"Embryos","url":"https://www.academia.edu/Documents/in/Embryos?f_ri=2256666"},{"id":1819400,"name":"Cohort Studies","url":"https://www.academia.edu/Documents/in/Cohort_Studies?f_ri=2256666"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation?f_ri=2256666"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_55602458" data-work_id="55602458" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/55602458/Identification_of_mammalian_blood_meals_in_mosquitoes_by_a_multiplexed_polymerase_chain_reaction_targeting_cytochrome_B">Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To date, no polymerase chain reaction diagnostic technique exists to directly identify mammalian blood meals from mosquitoes by sized DNA fragments following agarose gel electrophoresis. We have developed a vertebrate-specific multiplexed... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_55602458" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To date, no polymerase chain reaction diagnostic technique exists to directly identify mammalian blood meals from mosquitoes by sized DNA fragments following agarose gel electrophoresis. We have developed a vertebrate-specific multiplexed primer set based on mitochondrial cytochrome b to identify the mammalian blood hosts of field-collected mosquitoes. Although designed for the study of African malaria vectors, the application of this tool is not restricted to this disease system. Validation of this diagnostic technique on dried anopheline and culicine field specimens collected in Zambia and Mali demonstrated that blood meals could be identified 2-7 months after collection. Time course experiments showed that host DNA was detectable in frozen mosquito abdomens 24-30 hours post-feeding. Additionally, multiple blood meals from different mammals could be detected in a single mosquito. This diagnostic assay will be a valuable tool for identifying the blood meals of field-collected mosqu...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/55602458" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f91d6e6d0035a986c5c0bd9ae0d3f3c4" rel="nofollow" data-download="{"attachment_id":71398118,"asset_id":55602458,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/71398118/download_file?st=MTczMjM3NDI3MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37786032" href="https://independent.academia.edu/DouglasNorris">Douglas Norris</a><script data-card-contents-for-user="37786032" type="text/json">{"id":37786032,"first_name":"Douglas","last_name":"Norris","domain_name":"independent","page_name":"DouglasNorris","display_name":"Douglas Norris","profile_url":"https://independent.academia.edu/DouglasNorris?f_ri=2256666","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_55602458 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="55602458"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 55602458, container: ".js-paper-rank-work_55602458", }); 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We have developed a vertebrate-specific multiplexed primer set based on mitochondrial cytochrome b to identify the mammalian blood hosts of field-collected mosquitoes. Although designed for the study of African malaria vectors, the application of this tool is not restricted to this disease system. Validation of this diagnostic technique on dried anopheline and culicine field specimens collected in Zambia and Mali demonstrated that blood meals could be identified 2-7 months after collection. Time course experiments showed that host DNA was detectable in frozen mosquito abdomens 24-30 hours post-feeding. Additionally, multiple blood meals from different mammals could be detected in a single mosquito. This diagnostic assay will be a valuable tool for identifying the blood meals of field-collected mosqu...","downloadable_attachments":[{"id":71398118,"asset_id":55602458,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37786032,"first_name":"Douglas","last_name":"Norris","domain_name":"independent","page_name":"DouglasNorris","display_name":"Douglas 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