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class="iname">2020020335</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">各位大佬，现在培养了一些大肠杆菌，不知道其浓度，有无大肠杆菌各浓度对应的紫外分光光度计在600nm的标准曲线，比如10的7次方对应的600nm的吸光度，10的8次方，10的6次方 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>700</span> </li> <li> <i class="iconfont icon-comment"></i><span>14</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202107/14865538.html" target="_blank">sda-page电泳条带这个形状是什么问题？</a></h3> <a href="https://muchong.com/html/202107/14865538.html" target="_blank"> <div class="img_wrap"> <img src="//emuchvimg.oss-cn-qingdao.aliyuncs.com/img/2021/0716/w100h489513_1626414056_499.jpg.thumb.jpg" > </div> </a> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=489513" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">chenbo1027</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="p" >如图所示，sdspage的电泳条带，跑成这个样子是什么原因？ </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>1800</span> </li> <li> <i class="iconfont icon-comment"></i><span>36</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202107/14866857.html" target="_blank">细菌发酵液代谢产物测GCMS样品前处理</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=26642114" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">xieleiii</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">现在想把细菌发酵液送去测GCMS，想检测里面的代谢产物有哪些，样品应该进行怎样的处理呢？尽可能多的保留里面的化合物。 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>250</span> </li> <li> <i class="iconfont icon-comment"></i><span>5</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202107/14868499.html" target="_blank">细菌鉴定，建树后独为一支，如何确定它是什么菌</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=3391752" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="http://muchongavatar.xmcimg.com/data/avatar/e2/0f/3391752_1477400428.jpg"> </div> <div class="iname">yangzongying</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">最近从小龙虾体内分离出一株细菌，貌似是致病菌，在TCBS培养基上呈黑色圆形。通过测序之后，NJ法建立系统发育树，但是该菌独为一支，无法确定该菌的种属。还有什么好的方法来进行鉴定？请各位老师指导一下 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>250</span> </li> <li> <i class="iconfont icon-comment"></i><span>5</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202107/14872613.html" target="_blank">第一期微生物数据分析平台在线指导培训课开始预约报名</a></h3> <a href="https://muchong.com/html/202107/14872613.html" target="_blank"> <div class="img_wrap"> <img src="//emuchvimg.oss-cn-qingdao.aliyuncs.com/img/2021/0722/w68h26575598_1626943264_998.jpg.thumb.jpg" > </div> </a> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=26575598" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">GenesCloud</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="p" >开课通知！为了让大家快速全面掌握微生物数据分析平台及准确有效地分析测序结果，7月28日下午14:00中科助腾推出了线上【微生物数据分析平台】直播培训课第一期，由北京中科助... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>500</span> </li> <li> <i class="iconfont icon-comment"></i><span>10</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202107/14873305.html" target="_blank">发酵结果重复不出来</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=21988016" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">Luoshuang_</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">请问一下，构建了一个重组菌株，刚开始发酵和后面发酵的结果比对发现重复不出来，是什么原因？难道是传代太多次影响了菌株的活性吗？ </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>150</span> </li> <li> <i class="iconfont icon-comment"></i><span>3</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202107/14875255.html" target="_blank">感觉要毕不了业拉</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=25920082" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">花与喻橙</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">在做植物内生真菌的抑菌实验，最近感觉进行不下去啦，真菌都不能好好长了，培养基都换了好几个了，培养一周长了不到4厘米的菌落。真菌生长的速度都赶不上我做实验的态度。早之前就像换课题了，但老师不让，不知道要怎么办了 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>150</span> </li> <li> <i class="iconfont icon-comment"></i><span>3</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202107/14876436.html" target="_blank">无奈，给需要的人提供点光合细菌菌种或是交换培养方法</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=1236038" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">wsliuleilei</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">:p迫于生计，现将自己从市场上的好的产品中分离了几个光合细菌菌种，也有购买的沼泽红、深红标准菌株，提供给需要的人。这些菌种都是纯化过的，颜色、特性也各不相同，有的能够发酵罐规模化生产，有的分泌多糖丰富，菌液非常粘稠，有的颜色深红到发黑，菌种都是有偿提供。发酵罐... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>500</span> </li> <li> <i class="iconfont icon-comment"></i><span>10</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202107/14878642.html" target="_blank">关于金黄色葡萄球菌回补质粒pli50质粒构建的问题</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=5066123" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">Cbc_D</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">使用pli50构建目的基因的回补质粒时。使用pli50线性片段连接目的基因。该基因是只需要目的基因的全长？还是需要同时添加其相对应的启动子？才能起到基因回补的作用。 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>150</span> </li> <li> <i class="iconfont icon-comment"></i><span>3</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14882428.html" target="_blank">关于酶催化的求助！</a></h3> <a href="https://muchong.com/html/202108/14882428.html" target="_blank"> <div class="img_wrap"> <img src="//emuchvimg.oss-cn-qingdao.aliyuncs.com/img/2021/0801/w117h23020685_1627786875_628.jpg.thumb.jpg" > </div> </a> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=23020685" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="http://thirdqq.qlogo.cn/g?b=oidb&k=x3RKeIP5TcS6a4VR3QN4FQ&s=40&t=1556444029"> </div> <div class="iname">dengshen1</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="p" >求助各位老师、大神！！我的原料是这个3-二甲基氨基-1-(噻吩基)-1-丙酮盐酸盐，我想用酶进行水相催化的话，是不是要先把盐酸中和掉，如果中和的话是用氢氧化钠吗？各位请看... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>250</span> </li> <li> <i class="iconfont icon-comment"></i><span>5</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14884386.html" target="_blank">求助活死细菌染色实验</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=14156785" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/9f/a1/14156785_1575702585.jpg"> </div> <div class="iname">hhch96</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">求助哪里可以买到活死细菌染色实验用的染料？ </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>300</span> </li> <li> <i class="iconfont icon-comment"></i><span>6</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14884738.html" target="_blank">最棒的微生物教材《Brock Biology of Microorganisms》2021年第16版PDF 网盘链接</a></h3> <a href="https://muchong.com/html/202108/14884738.html" target="_blank"> <div class="img_wrap"> <img src="//emuchvimg.oss-cn-qingdao.aliyuncs.com/img/2021/0803/w89h4674404_1627974405_663.jpg.thumb.jpg" > </div> </a> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=4674404" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">nivea2016</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="p" >brockbiologyofmicroorganismssetsthestandardforaccuracy,impeccablescholarship,avisuall... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>23750</span> </li> <li> <i class="iconfont icon-comment"></i><span>475</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14885376.html" target="_blank">屎肠球菌用什么培养基保存</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=2013257" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/35/7a/2013257_1621474484.png"> </div> <div class="iname">lina陶</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">大神们，有没做屎肠球菌的，请问用什么培养基放在斜面保存 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>200</span> </li> <li> <i class="iconfont icon-comment"></i><span>4</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14890702.html" target="_blank">细菌平板照片拍摄方法</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=17679141" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/51/cc/17679141_1593937246.jpg"> </div> <div class="iname">李求到升仙</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">请问怎么样拍细菌培养基平板的照片比较好看 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>250</span> </li> <li> <i class="iconfont icon-comment"></i><span>5</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14891539.html" target="_blank">杀菌效率怎么计算</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=1519480" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="http://muchongavatar.xmcimg.com/data/avatar/98/1f/1519480.gif"> </div> <div class="iname">marverick</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">许多论文关于杀菌效率利用的公式是reduction=100*（A-B）/B。其中，A是阴性对照组的细菌数，B是含有杀菌剂样品的细菌数。按照这个公式，论文中不应该有阴性对照组的数据，因为它永远是0。但是论文中又有10分钟后对照组杀菌10%这样的数据。请问怎么回事... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>250</span> </li> <li> <i class="iconfont icon-comment"></i><span>5</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14891542.html" target="_blank">杀菌实验需要什么重复？</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=1519480" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="http://muchongavatar.xmcimg.com/data/avatar/98/1f/1519480.gif"> </div> <div class="iname">marverick</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">杀菌实验，每个样品重复3次，取平均值。这个重复实验是生物学重复还是技术重复？二者有什么区别？有要求吗？ </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>150</span> </li> <li> <i class="iconfont icon-comment"></i><span>3</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14893462.html" target="_blank">滤纸片法抑真菌实验</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=14119746" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/99/95/14119746_1550292490.jpg"> </div> <div class="iname">小学生们</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">请问各位大神，有做过滤纸片法抑真菌实验么，跪求具体实验操作。谢谢各位了 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>200</span> </li> <li> <i class="iconfont icon-comment"></i><span>4</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14893731.html" target="_blank">求助做过链霉菌接合转移的同学们呀</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=20931641" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="http://thirdqq.qlogo.cn/g?b=oidb&k=XsbVPHLtFR3Rs4jxgNKm6g&s=40&t=1557470935"> </div> <div class="iname">王一琇</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">我用PKC1139质粒进行属间接合转移，三个多月了都没有结合子生长不知道是什么问题呀。现在在进行条件摸索和体系建立。就是孢子过滤洗脱后40℃，45℃，50℃，55℃这几个条件热激10分钟后，28摄氏度震荡培养2.4.6小时后，与生长到OD600=0.4-0.5... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>450</span> </li> <li> <i class="iconfont icon-comment"></i><span>9</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14896086.html" target="_blank">毕赤酵母分泌表达蛋白，分子量变大?</a></h3> <a href="https://muchong.com/html/202108/14896086.html" target="_blank"> <div class="img_wrap"> <img src="//emuchvimg.oss-cn-qingdao.aliyuncs.com/img/2021/0813/bw141h20148043_1628824132_528.jpg.thumb.jpg" > </div> </a> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=20148043" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="http://thirdqq.qlogo.cn/g?b=oidb&k=LKf0ibWnh9MF9fzNABT7dnQ&s=40&t=1574324379"> </div> <div class="iname">马尚0</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="p" >我用目的基因连到载体pPICZaA,转到X33里，表达出来条带在17-20KD之间，理论上应该是14-15KD,这是什么原因啊?还有就是目的蛋白是连有组氨酸标签的，用镍柱... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>600</span> </li> <li> <i class="iconfont icon-comment"></i><span>12</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14899353.html" target="_blank">枯草芽孢杆菌发酵问题</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=3302711" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">无法无边</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">本人做枯草芽孢杆菌发酵，下罐的OD值都在一百多，测出的菌量都在100亿以上，但其中一罐OD值也在一百多，测出的菌量却只有20亿左右，是菌自溶了吗？还是其他原因，请大神帮忙分析一下。 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>200</span> </li> <li> <i class="iconfont icon-comment"></i><span>4</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14903862.html" target="_blank">微生物发酵后不分离菌体来提取化合物可以吗</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=20513314" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/27/49/20513314_1628673203.jpg"> </div> <div class="iname">哈果6666</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">微生物发酵后不分离菌体来提取化合物，全部带菌体和发酵液一起真空冷冻干燥，然后萃取分离纯化 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>400</span> </li> <li> <i class="iconfont icon-comment"></i><span>8</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14905369.html" target="_blank">如何把微生物样品寄送到国外</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=24336429" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/10/ae/24336429_1611725939.jpg"> </div> <div class="iname">daidai wu</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">请问有没有大佬把用于做微生物的样品寄送到国外啊？是需要出示什么许可吗？ </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>800</span> </li> <li> <i class="iconfont icon-comment"></i><span>16</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14907351.html" target="_blank">毕赤酵母蛋白表达不出来…</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=24675429" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">木杉1123</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">求助各位虫友们，我的毕赤酵母GS115+载体ppic9k，蛋白大小不到20kD，前面的步骤都做的没问题，也进过核酸电泳验证过了，可是到了蛋白表达这一步，就是不表达…跑蛋白电泳1，浓度太低，直接跑基本没东西。试过TCA和PEG浓缩之后，条带与空白对照没有相应条带... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>500</span> </li> <li> <i class="iconfont icon-comment"></i><span>10</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14911878.html" target="_blank">乳酸菌DNA保存在-20℃，有反复溶解、冷冻，会降解吗？</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=22417340" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/dc/0a/22417340_1625303536.png"> </div> <div class="iname">dinghui456</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">提前准备好的目标乳酸菌DNA，存在-20℃的冰箱，2周前与primer结合后，电泳能跑出阳性条带，期间因为实验其他不顺利反复解冻、溶解，最近已经跑不出阳性条带，这是降解了？我看其他帖子说细菌DNA4℃能保存2-3天，那解冻在20℃环境中，总共超过3小时，DNA... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>400</span> </li> <li> <i class="iconfont icon-comment"></i><span>8</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14913400.html" target="_blank">求助铜绿CRISPR Cas9基因敲除方法</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=22851202" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="http://thirdqq.qlogo.cn/g?b=oidb&k=3rRHxWzorEdf31E32EUwgA&s=40&t=1557253466"> </div> <div class="iname">阿哥是小黑</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">有没有哪位大神可告知细菌Cas9基因敲除，sgRNA如何设计？如果是用季泉江老师的课题组的体系最好。另，有没有哪位大神有季老师课题组pCasPA和pACRISPR质粒图谱的？ </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>250</span> </li> <li> <i class="iconfont icon-comment"></i><span>5</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14917177.html" target="_blank">求助TEM-1 E. coli (ATCC 35218)</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=4503771" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/0f/9d/4503771_1570532953.jpg"> </div> <div class="iname">青风微雨</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">请问哪个课题组有TEM-1E.coli(ATCC35218)，可购买或交换资源，感激不尽 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>200</span> </li> <li> <i class="iconfont icon-comment"></i><span>4</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202108/14917642.html" target="_blank">请教虫友们一个SEM观察葡萄球菌的问题</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=368490" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">westlover</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">本人在超亲水多孔表面培养球菌3h，其中部分试样含银，经固定、脱水后观察，不含银表面有细菌，含银表面完全看不到细菌，连破的球都看不到，已经重复了3次，都看不到。请问大家是什么原因啊？谢谢。操作流程：菌种为金黄葡萄球菌。细菌浓度选取1x105CFUml-1。取10... </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>200</span> </li> <li> <i class="iconfont icon-comment"></i><span>4</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202109/14922036.html" target="_blank">购买或者交换菌种</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=26439876" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/data/emuch_bbs_images/app/noavatar.png"> </div> <div class="iname">shuang530</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">各位虫友们，有偶发分枝杆菌，堪萨斯分枝杆菌，胞内分枝杆菌吗？购买或者资源交换，我这边有很多种菌 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>400</span> </li> <li> <i class="iconfont icon-comment"></i><span>8</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202109/14929057.html" target="_blank">如何获得枯草芽孢杆菌的芽孢悬浮液求求求</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=4180590" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/e2/8f/4180590_1551082433.png"> </div> <div class="iname">ajwx2017</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">如何获得枯草芽孢杆菌的芽孢悬浮液求求求求大佬告知天使托 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>950</span> </li> <li> <i class="iconfont icon-comment"></i><span>19</span> </li> </ul> </div> </div> </div><div class="list_item clearfix"> <h3 class="ititle"><a href="https://muchong.com/html/202109/14929102.html" target="_blank">如何用spss软件主成分分析得到PCA的结果图？</a></h3> <div class="icontent"> <a href="https://muchong.com/bbs/space.php?uid=4180590" target="_blank"> <div class="iuser"> <div class="avatar"> <img src="//muchongimg.xmcimg.com/oss2/avatar/e2/8f/4180590_1551082433.png"> </div> <div class="iname">ajwx2017</div> <div class="imotto"></div> </div> </a> <div class="itext"> <div class="nop">如何用spss软件主成分分析得到PCA的结果图？就是以factor1为横坐标，factor2为纵坐标。然后通过各个因子的得分得到的坐标图。结果怎么得到的？谢谢大家 </div> </div> <div class="iother clearfix"> <a href="" target="_blank"><span class="board_name">微生物</span></a> <ul> <li> <i class="iconfont icon-view"></i><span>400</span> </li> <li> <i class="iconfont icon-comment"></i><span>8</span> </li> </ul> </div> </div> </div> </div> <div class="page ui-pagination"> <ul><li class="goLast"><a>14355</a></li><li class="curPage">1</li><li class="pageTo" ><a href="/html/f367_2.html" pageto="2">2</a></li><li class="pageTo" ><a href="/html/f367_3.html" pageto="3">3</a></li><li class="pageTo" ><a href="/html/f367_4.html" pageto="4">4</a></li><li class="pageTo" ><a href="/html/f367_5.html" pageto="5">5</a></li><li class="pageTo" ><a href="/html/f367_6.html" pageto="6">6</a></li><li class="pageTo"><a href="/html/f367_2.html" >&rsaquo;&rsaquo;</a></li><li class="pageto"><a href="/html/f367_479.html">... 479</a></li></ul> </div> </div> <div class="w300 fr"> <div class="board_nav"> <div class="bhd"> 板块导航 </div> <div class="bbd"> <ul class="clearfix"> <li> <span>网络生活</span> <dl> <dd><a href="/html/f359.html">育儿交流</a></dd> <dd><a href="/html/f179.html">健康生活</a></dd> <dd><a href="/html/f208.html">有奖问答</a></dd> </dl> </li> <li> <span>资源共享</span> <dl> <dd><a 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