Non-Viral Gene Engineering With TcBuster

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<div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><strong>On This Page</strong><br><a href="#Applications">Applications</a> | <a href="#Protocols">Protocols</a> | <a href="#faq">FAQ</a> | <a href="#form">Contact an Expert</a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Applications"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Transform Your Cell Therapy Manufacturing With Non-Viral Gene Delivery</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p lang="EN-US"> </p><h3><em><strong>Fast, Scalable, and Cost-Effective Stable Gene Transfer Solution</strong></em></h3><p lang="EN-US">TcBuster is a large capacity transposon system that enables transfer and stable integration of multiple genes of interest. An effective alternative to viral vectors, TcBuster offers significant time and cost savings during the development of cell therapies, such as CAR T or CAR NK.</p><p lang="EN-US"> </p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="#form" class="btn btn-primary" type="link"> Contact an Expert </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="bMVSnmxWARM" params="rel=0" style="background-image: url('https://img.youtube.com/vi/bMVSnmxWARM/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/bMVSnmxWARM?autoplay=1" class="lty-playbtn" title="TcBuster(TM) - A next-generation non-viral gene delivery system"> <span class="lyt-visually-hidden">TcBuster(TM) - A next-generation non-viral gene delivery system</span> </a> </lite-youtube> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><figure class="responsive-figure-table"><table class="table table-bordered overflow-auto"><tbody><tr><td class="w-25" colspan="4"><h2>TcBuster Reagents and Uses</h2></td></tr><tr><td class="w-25" rowspan="3"><h4>mRNA Transposase</h4></td><td class="w-16"><a href="/p/cell-culture/tcbuster-m-transposase-mrna_tcb-001.1-100">TCB-001.1-100</a> (100 µg) </td><td class="w-16">100 tests at 1 µg each</td><td>- Useful for system evaluation</td></tr><tr><td class="w-16"><a href="/p/cell-culture/tcbuster-m-transposase-mrna_tcb-001.1-100">TCB-001.1-500</a> (500 µg)</td><td class="w-16">500 tests at 1 µg each</td><td class="w-100">- Useful for further development</td></tr><tr><td><a href="/p/cell-culture/tcbuster-m-gmp-transposase-mrna_tcb-001.1-gmp">TCB-001.1-GMP</a> (500 µg)</td><td>500 tests at 1 µg each</td><td>- GMP grade<br>- Useful for pre-IND process development and ex vivo clinical applications</td></tr><tr><td class="w-25" rowspan="3"><h4>TcBuster-Compatible Transposons</h4></td><td class="w-16"><a href="/p/cell-culture/tcbuster-transposon-cd19car-dhfr-egfp_tcbp001-100">TCBP001-100</a> (100 µg)</td><td class="w-16">25-100 tests at 1-4 µg each</td><td>- Multicistronic CAR-containing transposon<br>- Useful in cell therapy applications as an experimental control</td></tr><tr><td class="w-16"><a href="/p/cell-culture/tcbuster-transposon-insert-on-egfp_tcbp002-100">TCBP002-100</a> (100 µg)</td><td class="w-16">25-100 tests at 1-4 µg each</td><td>- Whole plasmid insertion of eGFP with no untransposed plasmid expression<br>- Useful in bioprocessing system evaluation as an experimental control</td></tr><tr><td class="w-16"><a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-tcbuster-molecular-guide.pdf">Custom Transposons</a></td><td class="w-16">Contact <a href="mailto:techsupport@bio-techne.com">techsupport@bio-techne.com</a> for more information </td><td>- For process development using your own therapeutic cargo<br>- Utilizes partnership with Aldevron™</td></tr><tr><td class="w-25"><h4>Anti-TcBuster-M Transposase Antibody     </h4></td><td class="w-16"><a href="/p/antibodies/tcbuster-m-transposase-antibody-1077035_mab11511">MAB11511</a> (25 µg, 100 µg)</td><td class="w-16">1 µg/mL for Western Blot<br>20 µg/mL for Simple Western</td><td>- Primary antibody detects TcBuster-M transposase enzyme in Western blotting applications</td></tr></tbody></table></figure><p lang="EN-US"> </p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>How Does the TcBuster System Compare to Virus-Based Systems?<a name="Comparison"></a></h2><p><a name="Why Use"></a></p><p>Gene transfer is a critical step in generating cell therapies and biologics. While viral vector-based engineering (e.g. lentivirus or AAV) has been the standard for delivering DNA cargo to cells, the TcBuster system provides an alternative, non-viral solution using standard electroporation methods. This system has several advantages over traditional viral options: </p><ul><li><p lang="EN-US">Reduced time to market and cost requirements for cell therapies</p></li><li><p lang="EN-US">Increased cargo capacity, high editing efficiency, and delivery of multiple genes in single vector</p></li><li><p lang="EN-US">Stable expression of DNA cargo with a de-risked insertional profile</p></li><li><p lang="EN-US">Strong and reliable supply chain for both RUO & GMP grade material</p></li><li><p lang="EN-US">Scale effortlessly from research to clinical and commercial stages with reduced batch-to-batch variability and no need for viral preps</p></li><li><p lang="EN-US">Flexible and non-exclusive license options plus robust technical and regulatory support<br> </p></li></ul></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Saving Time Means Saving Money: Get Your Therapy to Market Faster</h2></div> </div> </div> </div> <div> <div class="paragraph-image-banner banner_wrapper"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/services/non-viral-gene-delivery-v3.jpg" style="background-repeat: no-repeat"> <div class="text-align-center"> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/luminary-therapeutics-logo-v2.png" alt="Luminary Therapeutics Corporate Logo" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p><br> </p><h3>A Story of Success: Luminary Therapeutics</h3><p><a href="https://www.luminarytx.com/platform/" target="_blank"">Luminary Therapeutics</a> is using the TcBuster system to manufacture their cell therapies, and their LMY-920 program has <a href="https://www.kget.com/business/press-releases/ein-presswire/676190173/luminary-therapeutics-announces-fda-clearance-of-phase-i-ind-for-a-baff-car-to-treat-systemic-lupus-erythematosus-sle/" target="_blank" rel="noreferrer">passed 3 INDs </a>with TcBuster! At SITC 2024, Phase 1 results were reported for a <a href="https://jitc.bmj.com/content/12/Suppl_2/A282">BAFF CAR-T trial</a> using TcBuster, demonstrating the first successful clinical use of the system.</p><p><em>"Moreover, the novel, clinical-stage TcBuster transposon system is utilized to improve manufacturing timelines, efficiency, cost, and safety." </em></p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Protocols"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>TcBuster Protocols</h2><figure class="responsive-figure-table"><table class="table table-bordered overflow-auto"><thead><tr><th><h3>Cell Type</h3></th><th colspan="3"><h3>Electroporation Platform</h3></th></tr></thead><tbody><tr><td><h3>T Cells</h3></td><td><a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/tcbuster_lonza_protocol_inter.pdf">Lonza<sup>®</sup></a></td><td><a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/bio-techne-transposition-human-tp.pdf">MaxCyte<sup>®</sup></a></td><td><a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/tcbuster_neon_protocol_inter.pdf">Neon<sup>™</sup></a></td></tr><tr><td><h3>NK Cells</h3></td><td><a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-lonza-nk-protocol-v5.pdf">Lonza</a></td><td><a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-tp-maxcyte-nk-protocol.pdf">MaxCyte</a></td><td><a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-nk-neon-protocol.pdf">Neon</a> </td></tr><tr><td><h3>CHO-K1 Cells</h3></td><td colspan="3"><a href="https://resources.rndsystems.com/pdfs/brochures/tcbuster_cho-k1_neon_enhanced_product_usability.pdf">Neon</a> </td></tr></tbody></table></figure><p>TcBuster is an excellent choice for engineering cells on any electroporation platform.</p></div> </div> </div> <div> <section class="paragraph paragraph--type--full-width-banner-with-image mb-4 paragraph--view-mode--default paragraph-background-color--dark"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-bg-1000x405-bottomleft-1.png" style="background-repeat: no-repeat;"> <div> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2>TcBuster for Bioprocessing: <br>Compared to Random Integration<br> </h2><p>This platform is flexible! By adjusting TcBuster system parameters, you can achieve high copy number of your sequences in your CHO or HEK cells. The integrated cargo is high-expressing and stable over extended culture times.</p><p> </p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="#form" class="btn btn-primary" type="link"> Learn More </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/banners/v2_new-antibodies-hero-white.png" alt="New Antibodies Hero FG" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2> </h2><h2><strong>How Can Bio-Techne Support You and Your Projects? </strong></h2><h3><em>We are your partner in cell therapy - and we have solutions to your cell therapy roadblocks.</em></h3><ul><li><p lang="EN-US">Bio-Techne is here to stay, with over 40 years of scientific excellence</p></li><li><p lang="EN-US">We provide a strong supply chain, for both our research grade and GMP products</p></li><li><p lang="EN-US">We offer flexible licensing on our materials when you bring them the clinic or commercialization </p></li><li><p lang="EN-US">Regulatory support, with a DMF coming soon, and the ability to support worldwide customers with letters of authorization</p></li><li><p lang="EN-US">Robust technical service to support you while you evaluate the system</p><p lang="EN-US"> </p></li></ul></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="faq"></a></p></div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>Have More Questions About the TcBuster System?</h2></div> <a href="/therapeutic-manufacturing/gene-delivery-tcbuster/faq" > <button type="button" class="btn btn-primary"> See TcBuster FAQs </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><figure class="responsive-figure-table"><table class="table table-striped"><tbody><tr><td colspan="2"><strong>TcBuster Resources</strong></td><td colspan="2"><strong>Scientific Articles and Presentations</strong></td></tr><tr><td>Literature</td><td>Videos & Webinars</td><td>Papers</td><td>Posters & Abstracts</td></tr><tr><td><a href="/resources/literature/tcbuster-non-viral-gene-delivery-built-with-nanoplasmid">TcBuster-M + Nanoplasmid Vector System </a></td><td><a href="/resources/videos/tcbuster-transposon-gene-editing">Non-Viral Gene Delivery with TcBuster</a></td><td><a href="https://www.biorxiv.org/content/10.1101/2024.09.03.611042v2" target="_blank" rel="noreferrer">Bridge et al.</a></td><td><a href="https://jitc.bmj.com/content/12/Suppl_2/A282" target="_blank" rel="noreferrer">Caimi et al. BAFF CAR-T Study</a></td></tr><tr><td><a href="/resources/literature/non-viral-transposon-based-gene-engineering-platform-in-cell-therapy">Non-Viral Gene Engineering Whitepaper</a></td><td><a href="/resources/videos/non-viral-transposon-system-tcbuster">Transposon Gene Editing Mechanism</a></td><td><a href="https://www.isct-cytotherapy.org/article/S1465-3249(22)00739-3/pdf" target="_blank" rel="noreferrer">Gurney et al.</a></td><td><a href="/resources/literature/comparing-non-viral-transposon-system-to-lentiviral-transduction">TcBuster vs. Lentivirus in T Cells</a></td></tr><tr><td><a href="/resources/literature/lentiviral-transduction-non-viral-gene-delivery-car-t-manufacturing">TcBuster v. Lentivirus App Note</a></td><td><a href="/resources/webinars/non-viral-gene-engineering-with-tcbuster">Cell Therapy Development Webinar</a></td><td><a href="https://ashpublications.org/blood/article/138/Supplement%201/1725/480879/Tc-Buster-Transposon-Engineered-CLL-1-CAR-NK-Cells" target="_blank" rel="noreferrer">Gurney et al. (2)</a></td><td><a href="/resources/literature/genome-editing-immune-cells-multicistronic-cargo-tcbuster">TcBuster-M System in T Cells</a></td></tr><tr><td><a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-tcbuster-molecular-guide.pdf">Custom DNA Transposons Guide</a></td><td><a href="/resources/webinars/non-viral-manufacturing-of-gene-modified-car-t-therapies">CAR-T Manufacturing Webinar</a></td><td><a href="https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(24)00241-7" target="_blank" rel="noreferrer">Skeate et al.  </a></td><td> </td></tr><tr><td> </td><td> </td><td><a href="https://www.nature.com/articles/s41467-021-27853-w" target="_blank" rel="noreferrer">Wong et al.</a></td><td> </td></tr></tbody></table></figure></div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout 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