Codon Optimization | GENEWIZ from Azenta

<!DOCTYPE html>    <html lang="en">  <head> <title>Codon Optimization | GENEWIZ from Azenta</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="Azenta’s codon optimization tool produces codon optimized gene sequences for efficient expression in almost all host systems."> <meta name="keywords" content="genewiz, azenta life sciences, codon, codon optimization, codon bias, synthetic dna, gene sequences, codon optimized, transcriptional, translational efficiency, improve gene expression"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/Codon-Optimization/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/Codon-Optimization" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/Codon-Optimization" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Gene-Synthesis/Codon-Optimization" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Gene-Synthesis/Codon-Optimization" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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.gs-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .gs-new-container { text-align: center; margin-top: 0; } .gs-new-content { width: 90%; margin: 0 auto; } .gs-new-content { display: inline-block; } #gs-content-box-right, #gs-content-box-left { width: 100%; text-align: center; } #gs-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .gs-new-content { width: 90%; margin: 0 auto; } .gs-content-box img { width: 350px; } } </style> <div class="gs-new-container"> <div class="gs-new-content"> <div class="gs-content-box" id="gs-content-box-left"> <div> </div> <h1>Codon Optimization</h1> <div> </div> <p>As experts in synthetic DNA, we understand the importance of accurate gene synthesis and its subsequent expression, particularly in heterologous systems. A multitude of factors, including codon bias, can affect the transcriptional and translational efficiency of a gene in a host organism. Azenta Life Sciences, formerly GENEWIZ’s codon optimization tool considers these critical factors to produce codon optimized gene sequences for efficient expression in almost all host systems. </p> <p><span style="text-align: center;"> </span></p> </div> <div class="gs-content-box" id="gs-content-box-right"> <p style="text-align: center;"><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Gene-Synthesis" target="_blank"><img alt="" src="/-/media/Images/Codon-Banner-Right-Ad042622.ashx?la=en&hash=9B5FF0F7F1895E77D90D0549E278AC71E7BE371E" /></a> </p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; padding: 0; } .gs-page-container { background: #ffffff; padding: 0 0; margin-top: 30px; } .gs-page-container h1 { margin: 0; color: #000000; } .gs-new-content p { color: #000000; } .gs-new-content { width: 1170px; margin: 0 auto; } .gs-content-box { display: inline-block; /*width: 50%;*/ } #gs-content-box-left { width: 40%; text-align: left; vertical-align: top; } #gs-content-box-right { width: 55%; text-align: center; vertical-align: middle; } .gs-content-box img { width: 450px; text-align: center; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .gs-page-container { text-align: center; margin-top: 0; } .gs-new-content { width: 90%; margin: 0 auto; } .gs-new-content { display: inline-block; } #gs-content-box-right, #gs-content-box-left { width: 100%; text-align: center; } #gs-content-box-right { width: 100%; text-align: center; } } @media only screen and (max-width: 1170px) { .gs-new-content { width: 90%; margin: 0 auto; } .gs-content-box img { width: 350px; } } </style> <div class="gs-page-container"> <div class="gs-new-content"> <h2>Codon Optimized Synthetic GENES</h2> <div class="gs-content-box" id="gs-content-box-left"> <p>Codons represent the genetic code that transfers information from genes to mRNA to protein. Both redundancy and evolutionary constraints, including the availability of tRNA isoacceptors, TATA box, Shine-Dalgarno sequences, and more, result in preferential usage of one codon over another for the same amino acid. This phenomenon is known as codon bias.</p> <p>Codon optimization is a process used to improve gene expression and increase the translational efficiency of a gene of interest by accommodating codon bias of the host organism. </p> <p>Download your complimentary copy of our tech note,<em> <a href="https://web.azenta.com/codon-optimization-technote?__hstc=251210969.8aa0fe9991d7e88ea77f60833a941cdc.1645541971541.1650376207975.1650441251321.94&__hssc=251210969.64.1650441251321&__hsfp=3274392515">Enhancing Protein Expression by Leveraging Codon Optimization</a></em>, to learn how you can leverage Azenta’s free codon optimization tool to enhance your protein expression.</p> </div> <div class="gs-content-box" id="gs-content-box-right"> <p style="text-align: center;"><img alt="Protein Expression" src="/-/media/Images/Services/GS/photos/CodonOptimizationTranscriptionTranslation.ashx?la=en&hash=0DD94A990F84A17CA51E67CE2AE6CF7B0DB0ED22" /></p> <p style="text-align: center;">Schematic representation of protein expression</p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .headline-center { padding-top: 0; } i.icon-custom { border: none; } .tech-resources-main { padding-bottom: 30px; z-index:9; position: relative; } .tech-resources-container { width: 1170px; margin: 0 auto; background: #55c8d2; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div class="tech-resources-main"> <div class="tech-resources-container"> <div class="tech-resource"> <a id="crispr-tech-resources" class="anchor"></a> <div class="resource resource-left"> <img alt="" src="/-/media/Images/Tech-Note-Thumbnail-V2.ashx?la=en&hash=C66A0C43539726ED6657F514E8392B27AD1C032F" /> </div> <div class="resource resource-right"> <h3>Tech Note: Enhancing Protein Expression by Leveraging Codon Optimization</h3> <br /> <a rel="noopener noreferrer" href="https://web.genewiz.com/tech-note/codon-optimization" target="_blank"><button>Download</button></a> <p> </p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2> Optimize and Order In Minutes</h2> <p style="text-align:left;">The introduction of optimization algorithms, a type of artificial intelligence, has significantly streamlined the process of designing a DNA sequence to optimize protein expression in target organisms. When you partner with Azenta, you get access to our <a href="https://clims4.genewiz.com/RegisterAccount/Login?returnUrl=https://clims4.local.genewiz.com/Toolbox/CodonOptimization">free codon optimization algorithm tool</a> that recognizes and optimizes key parameters to stabilize DNA sequences and improve gene expression. Choose from dozens of expression systems and hundreds of restriction enzyme sites while we ensure that your sequence is tailored to your research project.</p> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-10 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></em></div> <div class="content-boxes-in-v3"> <span style="font-weight: 700;">High protein expression</span> – Azenta’s algorithm analyzes a wide range of data to optimize critical parameters and significantly improve protein expression levels. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></em></div> <div class="content-boxes-in-v3"> <span style="font-weight: 700;">Difficult sequence normalization</span> – Our codon optimization tool can normalize difficult sequences to remove unfavorable regions and reduce local GC content to desired percentages. </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></em></div> <div class="content-boxes-in-v3"> <span style="font-weight: 700;">Comprehensive codon usage tables</span> – Species-specific codon usage tables allow the tool to identify and replace low-frequency codons with high-frequency codons for all major host organisms. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></em></div> <div class="content-boxes-in-v3"> <span style="font-weight: 700;">Ph.D.-level expertise and support</span> – Our dedicated team of scientists provide consultations and support for all your experimental needs, from start to finish. </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style> .oligo-how-to-order ul{display:none;} button { padding: 10px 20px 10px 20px; 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padding: 30px 0; } .fg-new-container h1 { margin: 0; color: #000000; } .fg-new-content p { color: #000000; } .fg-new-content { width: 1170px; margin: 0 auto; } .fg-content-box { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #fg-content-box-left { width: 100%; text-align: left; padding: 30px 0; } #fg-content-box-right { width: 39%; text-align: right; } .fg-content-box img { width: 400px; } .fg-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; text-align: center; } .fg-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .fg-new-container { text-align: center; margin-top: 0; } .fg-new-content { width: 90%; margin: 0 auto; } .fg-new-content { display: inline-block; } #fg-content-box-right, #fg-content-box-left { width: 100%; text-align: center; } #fg-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .fg-new-content { width: 90%; margin: 0 auto; } .fg-content-box img { width: 350px; } } </style> <div class="fg-new-container"> <div class="fg-new-content"> <div class="fg-content-box" id="fg-content-box-left"> <p>Backed by our 99.9% completion rate, Azenta synthesizes naturally occurring sequences of any length or complexity for your protein and antibody engineering, CRISPR gene editing, and more. Rather than spending your valuable time on DNA cloning, Azenta will custom clone your codon-optimized genes into your vector for optimal protein expression. We also offer a wide range of synthetic DNA solutions for your research, including gene fragment synthesis, synthetic DNA libraries, oligo synthesis, and CRISPR synthetic solutions.</p> <p>Azenta’s streamlined processes and protocols allow us to deliver timely and dependable results with proactive Ph.D.-level project support every step of the way. It's no wonder top research labs, pharmaceutical, and biotechnology companies around the world rely on Azenta for all of their synthetic DNA needs.</p> </div> </div> </div> </div>  <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> *Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. 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href='/en/Public/Company'>COMPANY</a></li><li class='FootNav2'><a href='/en/Public/Company/About-Us'>About GENEWIZ</a></li><li class='FootNav2'><a href='https://www.azenta.com/company/about-azenta/company-history'>About Azenta</a></li><li class='FootNav2'><a href='https://www.azenta.com/events'>News and Events</a></li><li class='FootNav2'><a href='https://careers.azenta.com/'>Careers<br></a></li><li class='FootNav2'><a href='/en/Public/Company/Locations-and-Hours'>Locations and Hours</a></li><li class='FootNav2'><a href='/en/Public/Company/Contact-GENEWIZ'>Contact Us</a></li><li class='FootNav2'><a href='/en/Public/Company/Policies'>Policies</a></li><li class='FootNav2'><a href='/en/Public/Company/Partnership-Solutions'>Partnership Solutions</a></li><li class='FootNav2'><a href='https://web.azenta.com/newsletter'>Subscribe to our newsletter</a></li></ul></div> </div> </div>  <div class="container text-center"> <div class="row"> <div class="col-sm-12"> <h3 style="color: white;">Azenta 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