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<!DOCTYPE html> <html class="client-nojs" lang="en" dir="ltr"> <head> <meta charset="UTF-8"/> <title>Methods - SGD-Wiki</title> <script>document.documentElement.className = document.documentElement.className.replace( /(^|\s)client-nojs(\s|$)/, "$1client-js$2" );</script> <script>(window.RLQ=window.RLQ||[]).push(function(){mw.config.set({"wgCanonicalNamespace":"","wgCanonicalSpecialPageName":false,"wgNamespaceNumber":0,"wgPageName":"Methods","wgTitle":"Methods","wgCurRevisionId":403549,"wgRevisionId":403549,"wgArticleId":18043,"wgIsArticle":true,"wgIsRedirect":false,"wgAction":"view","wgUserName":null,"wgUserGroups":["*"],"wgCategories":[],"wgBreakFrames":false,"wgPageContentLanguage":"en","wgPageContentModel":"wikitext","wgSeparatorTransformTable":["",""],"wgDigitTransformTable":["",""],"wgDefaultDateFormat":"dmy","wgMonthNames":["","January","February","March","April","May","June","July","August","September","October","November","December"],"wgMonthNamesShort":["","Jan","Feb","Mar","Apr","May","Jun","Jul","Aug","Sep","Oct","Nov","Dec"],"wgRelevantPageName":"Methods","wgRelevantArticleId":18043,"wgRequestId":"307a88ecad23656a1b9b1521","wgIsProbablyEditable":false,"wgRelevantPageIsProbablyEditable":false,"wgRestrictionEdit":[],"wgRestrictionMove":[]});mw.loader.state({"site.styles":"ready","noscript":"ready","user.styles":"ready","user":"ready","site":"ready","user.options":"ready","user.tokens":"loading","mediawiki.legacy.shared":"ready","mediawiki.legacy.commonPrint":"ready","mediawiki.sectionAnchor":"ready","mediawiki.skinning.interface":"ready","skins.vector.styles":"ready"});mw.loader.implement("user.tokens@1y0h9gd",function($,jQuery,require,module){/*@nomin*/mw.user.tokens.set({"editToken":"+\\","patrolToken":"+\\","watchToken":"+\\","csrfToken":"+\\"}); });mw.loader.load(["mediawiki.page.startup","mediawiki.user","mediawiki.hidpi","mediawiki.page.ready","mediawiki.searchSuggest","skins.vector.js"]);});</script> <link rel="stylesheet" href="/load.php?debug=false&lang=en&modules=mediawiki.legacy.commonPrint%2Cshared%7Cmediawiki.sectionAnchor%7Cmediawiki.skinning.interface%7Cskins.vector.styles&only=styles&skin=vector"/> <script async="" src="/load.php?debug=false&lang=en&modules=startup&only=scripts&skin=vector"></script> <meta name="ResourceLoaderDynamicStyles" content=""/> <link rel="stylesheet" href="/load.php?debug=false&lang=en&modules=site.styles&only=styles&skin=vector"/> <meta name="generator" content="MediaWiki 1.31.14"/> <link rel="shortcut icon" href="/favicon.ico"/> <link rel="search" type="application/opensearchdescription+xml" href="/opensearch_desc.php" title="SGD-Wiki (en)"/> <link rel="EditURI" type="application/rsd+xml" href="https://wiki.yeastgenome.org/api.php?action=rsd"/> <link rel="alternate" type="application/atom+xml" title="SGD-Wiki Atom feed" href="/index.php?title=Special:RecentChanges&feed=atom"/>  </head> <body class="mediawiki ltr sitedir-ltr mw-hide-empty-elt ns-0 ns-subject page-Methods rootpage-Methods skin-vector action-view"> <div id="mw-page-base" class="noprint"></div> <div id="mw-head-base" class="noprint"></div> <div id="content" class="mw-body" role="main"> <a id="top"></a> <div class="mw-indicators mw-body-content"> </div> <h1 id="firstHeading" class="firstHeading" lang="en">Methods</h1> <div id="bodyContent" class="mw-body-content"> <div id="siteSub" class="noprint">From SGD-Wiki</div> <div id="contentSub"></div> <div id="jump-to-nav" class="mw-jump"> Jump to: <a href="#mw-head">navigation</a>, <a href="#p-search">search</a> </div> <div id="mw-content-text" lang="en" dir="ltr" class="mw-content-ltr"><div class="mw-parser-output"><ul><li><a rel="nofollow" class="external text" href="http://wiki.yeastgenome.org/index.php/Software">Software tools</a> created by members of the scientific community.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://research.fhcrc.org/breeden/en/methods.html">Yeast Lab Protocols</a> from the Breeden Lab, Fred Hutchinson Cancer Research Center.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://research.fhcrc.org/gottschling/en/protocols/yeast-protocols.html">Yeast Lab Protocols</a> from the Gottschling Lab, Fred Hutchinson Cancer Research Center.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://gasch.genetics.wisc.edu/protocols.html">Yeast & Microarray Protocols</a> from the Gasch Lab, University of Wisconsin-Madison.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://home.cc.umanitoba.ca/~gietz/">The Definitive Yeast Transformation Homepage</a> from the University of Manitoba.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://fangman-brewer.genetics.washington.edu/index.html">DNA Replication Protocols</a> from the Fangman and Brewer Labs, University of Washington.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://cmgm.stanford.edu/pbrown/mguide/index.html">The MGuide. Version 2.0</a>, a complete guide to microarraying for the molecular biologist, from the Brown Lab, Stanford University.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://gasch.genetics.wisc.edu/protocols/Gasch_MethEnzym.pdf">Guide to Yeast Microarray Experiments</a>, by Audrey Gasch, from <u>Guide to Yeast Genetics and Molecular and Cellular Biology</u>, Methods in Enzymology (2002)</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://depts.washington.edu/yeastrc/">The NCRR Yeast Resource Center</a> at the University of Washington facilitates the study of yeast protein complexes by providing access to techniques such as mass spectrometry, two-hybrid arrays, deconvolution fluorescence microscopy and protein structure prediction.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://genome.cshlp.org/content/7/12/1174.full">PCR-Based Allele Replacement</a> (Erdeniz <i>et al.</i> (1997) Genome Res. 7:1174-1183). Available to the public without password restriction and at no charge, through a special arrangement with Genome Research arranged by Rodney Rothstein (<i>see <a href="/index.php/CommunityW303.html" title="CommunityW303.html">detailed notes</a> from RR and Stephan B盲rtsch on the W303 strain; derivatives were used in the study</i>).</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://dunham.gs.washington.edu/home.shtml">Chemostat Manual</a> from Maitreya Dunham's lab, University of Washington.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://www.phys.ksu.edu/gene/chapters.html">A Classroom Guide To Yeast Experiments</a> from The GENE project at Kansas State University based on <a rel="nofollow" class="external text" href="http://www.rushessay.com">essay writing</a>.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://openwetware.org/wiki/The_mRNA_Decay_Resource:Protocols_and_Resources">Protocols and Resources</a> from the RNA Decay Resource, a resource for the sharing of information, know-how, and wisdom among researchers who study mRNA decay.</li></ul> <ul><li><a href="/index.php/SGA" title="SGA">SGA</a> protocols and scoring software.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://Vadlo.com/">Molecular Biology Protocols Search Engine</a> from Life in Research, LLC. Also life sciences databases, online tools, software, and powerpoints. For example <a rel="nofollow" class="external text" href="http://www.vadlo.com/b/q?k=Yeast+Protocols&rel=0">Yeast protocols</a>, <a rel="nofollow" class="external text" href="http://www.vadlo.com/b/q?k=Yeast&rel=3">Yeast databases</a></li></ul> <ul><li><a rel="nofollow" class="external text" href="http://h.web.umkc.edu/honigbergs/yeastcommunity/concept.html.html">Yeast Community Site</a> from Honigberg Lab, University of Missouri Kansas City.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://www.magusto.com/">Concours Site</a> from concours Lab, University of Mousseaux sur seine (protocol in French).</li></ul> <ul><li><a href="/index.php/Yeast_Cell_Micromanipulation" title="Yeast Cell Micromanipulation">Yeast Cell Micromanipulation</a> Protocol for Making Support Rod with Fiber Optic Needle Attached from Cora Styles</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://yeastgenome.org/cgi-bin/geneticData/displayTwoPoint?">Genetic Mapping (Two-Point) Data</a> for yeast genes.</li> <li><a href="/index.php?title=GRSandPRIMED&action=edit&redlink=1" class="new" title="GRSandPRIMED (page does not exist)">PRIMED</a>: Complete primer set for deleting and C-terminally tagging every protein-coding and non-coding RNA gene in <i>S. cerevisiae</i>.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://www.babraham.ac.uk/our-research/epigenetics/jon-houseley/protocols">Yeast Protocols</a> particularly DNA/RNA analysis, from the Houseley Lab at the Babraham Institute. Updated May 2018.</li></ul> <ul><li><a rel="nofollow" class="external text" href="http://cshprotocols.cshlp.org/site/recipes/">Cold Spring Harbor Protocols</a> Alphabetical list of recipes for techniques and media (see 'Y' for example)</li></ul>   </div>  </div> <div class="printfooter"> Retrieved from "<a dir="ltr" href="https://wiki.yeastgenome.org/index.php?title=Methods&oldid=403549">https://wiki.yeastgenome.org/index.php?title=Methods&oldid=403549</a>" </div> <div id="catlinks" class="catlinks catlinks-allhidden" data-mw="interface"></div> <div class="visualClear"></div> </div> </div> <div id="mw-navigation"> <h2>Navigation menu</h2> <div id="mw-head"> <div id="p-personal" role="navigation" class="" aria-labelledby="p-personal-label"> <h3 id="p-personal-label">Personal tools</h3> <ul> <li id="pt-login"><a href="/index.php?title=Special:UserLogin&returnto=Methods" title="You are encouraged to log in; however, it is not mandatory [o]" accesskey="o">Log in</a></li> </ul> </div> <div id="left-navigation"> <div id="p-namespaces" role="navigation" class="vectorTabs" aria-labelledby="p-namespaces-label"> <h3 id="p-namespaces-label">Namespaces</h3> <ul> <li id="ca-nstab-main" class="selected"><span><a href="/index.php/Methods" title="View the content page [c]" accesskey="c">Page</a></span></li> <li id="ca-talk" class="new"><span><a href="/index.php?title=Talk:Methods&action=edit&redlink=1" rel="discussion" title="Discussion about the content page (page does not exist) [t]" accesskey="t">Discussion</a></span></li> </ul> </div> <div id="p-variants" role="navigation" class="vectorMenu emptyPortlet" aria-labelledby="p-variants-label"> <h3 id="p-variants-label"> <span>Variants</span> </h3> <div class="menu"> <ul> </ul> </div> </div> </div> <div id="right-navigation"> <div id="p-views" role="navigation" class="vectorTabs" aria-labelledby="p-views-label"> <h3 id="p-views-label">Views</h3> <ul> <li id="ca-view" class="selected"><span><a href="/index.php/Methods">Read</a></span></li> <li id="ca-viewsource"><span><a href="/index.php?title=Methods&action=edit" title="This page is protected.
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