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Jess Automated Western Blot System (004-650) by ProteinSimple, Part of Bio-Techne

<!DOCTYPE html> <html lang="en" dir="ltr" prefix="og: https://ogp.me/ns#"> <head> <meta charset="utf-8" /> <noscript><style>form.antibot * :not(.antibot-message) { display: none !important; }</style> </noscript><meta name="applicable-device" content="pc,mobile" /> <link rel="canonical" href="https://www.bio-techne.com/p/simple-western/jess-automated-western-blot-system_004-650" /> <meta property="og:site_name" content="Bio-Techne" /> <meta property="og:type" content="Website" /> <meta property="og:url" content="https://www.bio-techne.com/" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <script type="application/ld+json">{ "@context": "https://schema.org", "@graph": [ { "@type": "WebPage", "@id": "https://www.bio-techne.com/p/simple-western/jess-automated-western-blot-system_004-650", "breadcrumb": { "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Home", "item": "https://www.bio-techne.com/" }, { "@type": "ListItem", "position": 2, "name": "Simple Western", "item": "https://www.bio-techne.com/search?keywords=Simple%20Western\u0026category_attr=Simple%20Western" } ] } } ] }</script> <meta http-equiv="x-dns-prefetch-control" content="on" /> <meta http-equiv="x-ua-compatible" content="ie=edge" /> <meta name="keywords" content="004-650, Jess, Wes, Automated Western Blot, Automated Western Blotting, Westerns, capillary-based immunoassays, capillary based immunoassays, fluorescent western blot, immunoassay, protein separation, immunodetection" /> <meta name="description" content="Purchase ProteinSimple a Bio-Techne Brand, Jess Automated Western Blot System. Automate your workflow." /> <script type="application/ld+json" id="json-ld">[{"@context":"http://schema.org","@type":"Product","additionalProperty":[],"aggregateRating":{"@type":"AggregateRating","ratingValue":5,"reviewCount":1,"worstRating":"1","bestRating":"5"},"alternateName":"","brand":{"@type":"Brand","name":"ProteinSimple"},"description":"ProteinSimple\u2019s Jess\u2122 is a Simple Western\u2122 instrument, an innovative technology from Bio-Techne, that performs capillary-based immunoassays and fully automated Western blot analysis, taking the complexity out of traditional immunoassays like Western blot and ELISA and filling in their shortcomings.","image":[{"@type":"ImageObject","url":"https://resources.rndsystems.com/images/datasheets/004-650_jess-system-with-pc-simple-western-2842020112552.png","description":"Jess from ProteinSimple, a Bio-Techne brand, automates the protein separation &amp; immunodetection of traditional Western blotting. 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id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1281">Custom Cell &amp; Gene Therapy Services</a> <a href="/services/custom-compound-library-service" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2126">Custom Compound Library Services</a> <a href="/services/custom-elisa-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/261">Custom ELISA Services</a> <a 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Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/certified-service-provider-program" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5446">Certified Service Provider (CSP) Program</a> <a href="/resources/instrument-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5471">Contract Services</a> <a href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item 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ProteinSimple, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">004-650</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="reviews_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="reviews_quicklink"> <a href="#reviews" id="review_count_quicklink" class="reviews" data-tab="2"> <span class="review_stars review_stars_5"></span> <span class="font-weight-bold"></span> (1) </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <a href="#product-documents" class="arena_link product-documents" rel="nofollow">Product Documents</a> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-proteinsimple.svg" alt="ProteinSimple, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> </div> <div class="row mb-4"><div class="col-xl-3 order-xl-first" id="image_wrapper"><div id="images_summary"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="proteinsimple"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.rndsystems.com/images/datasheets/004-650_jess-system-with-pc-simple-western-2842020112552.png" alt="Jess Simple Western System" title="Jess - Chemiluminescent &amp; Fluorescent Western Blotting | ProteinSimple" data-caption="Jess from ProteinSimple, a Bio-Techne brand, automates the protein separation &amp; immunodetection of traditional Western blotting. Multiplex with chemiluminescence &amp; fluorescence channels. " data-badges="" data-application="Simple Western" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(2)</span> </div> </div> </div> <i data-src="https://resources.rndsystems.com/images/datasheets/004-650_jess-system-with-pc-1912024113810.png" data-alt="Multiplexed analysis of total AKT and pAKT with total protein normalization using Jess" title="Multiplexed analysis of total AKT and pAKT with total protein normalization using Jess." data-caption="(A) Lysates from Jurkat cells either untreated (-) or treated (+) with calyculin A were analyzed at 0.25 mg/mL and 0.15 mg/mL final concentrations, respectively. Each lysate was probed for total AKT with Stellar IR (green bands) and pAKT with Stellar NIR (red bands). (B) The Stellar Total Protein Assay was simultaneously performed in the same lanes shown in panel A." data-badges="" data-application="Simple Western" data-width="250" data-height="250" class="none"></i> </div> </div> </article> </div> </div> <div class="col-xl-9 order-xl-first" id="key_benefits_buttoon_wrapper"> <h3>Jess Provides Fully Automated Western Blot Analysis</h3> <article class="product-data-wrapper "> ProteinSimple’s Jess™ is a Simple Western™ instrument, an innovative technology from Bio-Techne, that performs capillary-based immunoassays and fully automated Western blot analysis, taking the complexity out of traditional immunoassays like Western blot and ELISA and filling in their shortcomings. </article> <div id="pp-orderblock-buttons"><div class="float-right mt-3"> <a class="btn a___button-primary m-1 mt-3 col-xs-4" href="/p/instruments/simple-western/request-quote?productName=Jess%20Automated%20Western%20Blot%20System&amp;catalogNumber=004-650" target="_self"> Request Pricing </a> </div> </div> </div> <div class="col-xl-3" id="product_ads_wrapper"><article class="product-data-wrapper"> </article> </div> </div> </div> <div><div id="instrument_meet_section"><div id="instrument_meet_section" class="top_region"> <div class="row"> <div class="col-md-6"> <h3 class="mb-2">How Does Simple Western with Jess Work?</h3> Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Just load your samples and reagents into the microplate, and Jess does the rest. She separates your protein by size and precisely manages antibody additions, incubations, washes and even the detection steps. Come back to fully quantitative results in just 3 hours. With Jess’s chemiluminescent and best-in-class fluorescent detection you’ll get picogram-level sensitivity, built-in total protein normalization, and highly reproducible ELISA-like results with molecular weight characterization. ProteinSimple’s Jess automated Western blot system - she’s like Western blot meets ELISA in one. </div> <div class="col-md-6" > <div class="embed-responsive embed-responsive-16by9"> <iframe width="560" height="315" src="https://www.youtube.com/embed/qoH2yqfbqoo?si=KtOrE7TGaMBbnfB1" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen> </iframe> </div> </div> </div> </div> </div> </div> <div><div id="instrument_additional_section"> <div class="paragraph paragraph--type--product-page-content paragraph--view-mode--full"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/testimonials/simple-western-cst-antibody-validation" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/headshots/fyp-Chris-LaBreck-200x200.png" alt="Chris LaBreck, Ph.D., Senior Product Scientist, Cell Signaling Technology" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/testimonials/simple-western-cst-antibody-validation" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p><em>"Using Jess has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform."</em><br> &nbsp;- Chris LaBreck, Ph.D., Senior Product Scientist, Cell Signaling Technology (CST)</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/testimonials/simple-western-cst-antibody-validation" > </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/testimonials/unraveling-neurodegenerative-diseases-with-jess" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-nicolas-g-bazan.png" alt="Nicolas Bazan thumbnail" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/testimonials/unraveling-neurodegenerative-diseases-with-jess" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p><em>"It saves so much time! It takes away so much of the pipetting and manipulation error. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities."</em><br> <em>&nbsp;-&nbsp;</em>Nicolas G. Bazan, M.D., Ph.D., Director, LSUHSC Neuroscience Center of Excellence</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/testimonials/unraveling-neurodegenerative-diseases-with-jess" > </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/testimonials/protac-drug-discovery-jess" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-rachel-doidge.png" alt="Rachel Doidge thumbnail" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/testimonials/protac-drug-discovery-jess" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p><em>"Our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."</em><br> &nbsp;- Rachel Doidge Ph.D., Senior Research Scientist, Aurelia Bioscience</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/testimonials/protac-drug-discovery-jess" > </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-default"> <div> <a href="/p/instruments/simple-western/request-quote" > <button type="button" class="btn btn-primary"> Request Pricing </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4 p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/certified-service-provider-badge1.png" alt="icon associated with a certified service provider" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div><h3>Jess Certified Service Provider Program</h3></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><h4>Find an Expert to Process Your Samples with Jess</h4> <p>Our CSP program combines dedicated expertise with Bio-Techne’s elite&nbsp;<strong><a href="https://www.bio-techne.com/brands/proteinsimple">instrument portfolio</a></strong>&nbsp;including <strong>Jess </strong>to create a robust solution for effective, reliable sample analysis. The service providers listed below have successfully completed Bio-Techne’s CSP training program and are certified to run your samples on Bio-Techne instruments. &nbsp;</p></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/services/certified-service-provider-program" class="btn btn-primary" type="link"> Learn About Our CSP Program </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="tab-content"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="about-jess-automated-western-blot-system-tab-tab" href="#about-jess-automated-western-blot-system-tab" data-toggle="tab" role="tab" aria-controls="region-4171591922717" aria-selected="true" >About Jess Automated Western Blot System</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-reviews--tab-tab" href="#citations-reviews--tab" data-toggle="tab" role="tab" aria-controls="region-8961620056042" aria-selected="false" >Citations / Reviews (1) / Testimonials (32)</a> </li> </ul> <div class="tab-content rounded-bottom border-left border-right border-bottom mb-3" id="ProductTabContent"><div class="tab-pane fade show active mx-4" id="about-jess-automated-western-blot-system-tab" data-tab-label="About Jess Automated Western Blot System" role="tabpanel" aria-labelledby="about-jess-automated-western-blot-system-tab-tab"><div class="product_details_anchors"><div class="product-pages-anchors nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"><li id="how-can-jess-automated-western-blot-system-help-you_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor active" href="#how-can-jess-automated-western-blot-system-help-you" id="how-can-jess-automated-western-blot-system-help-you_li" >How Can Jess Automated Western Blot System Help You?</a> </li> <li id="how-does-jess-automated-western-blot-system-work_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#how-does-jess-automated-western-blot-system-work" id="how-does-jess-automated-western-blot-system-work_li" >How Does Jess Automated Western Blot System Work?</a> </li> <li id="applications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#applications" id="applications_li" >Applications</a> </li> <li id="products-compatible-with-jess-automated-western-blot-system_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#products-compatible-with-jess-automated-western-blot-system" id="products-compatible-with-jess-automated-western-blot-system_li" >Products Compatible with Jess Automated Western Blot System</a> </li> <li id="specifications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#specifications" id="specifications_li" >Specifications</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents" href="#product-documents" id="product-documents_li" >Product Documents</a> </li> </div> <div id="how-can-jess-automated-western-blot-system-help-you" class="nav-link px-0" data-anchor-label="How Can Jess Automated Western Blot System Help You?"><table class="product-data-wrapper tablesaw tablesaw-stack table" id="data_image_table_wrapper"><h3>How Can Jess Automated Western Blot System Help You?</h3><article class="product-data-wrapper"> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_jess-system-with-pc-western-blot-1812022123557.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_jess-system-with-pc-western-blot-1812022123557.png" alt="Stellar High sensitivity picogram level protein detection" title="How Sensitive is Protein Detection?" data-caption="Jess offers market-leading protein detection sensitivity for Western blotting workflows. Choose between chemiluminescence detection or fluorescence detection with Stellar NIR / IR modules, all while detecting your protein targets with low pg sensitivity." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>How Sensitive is Protein Detection?</h4> <p> Jess offers market-leading protein detection sensitivity for Western blotting workflows. Choose between chemiluminescence detection or fluorescence detection with Stellar NIR / IR modules, all while detecting your protein targets with low pg sensitivity. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-fast-results.jpg" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-fast-results.jpg" alt="Automated Western Blot Analysis with Jess" title="Can I Get Faster Results than a Western Blot?" data-caption="With Jess, it&#039;s pipette, run &amp; done! Simply load your sample, antibodies and reagents into the plate, insert your plate and cartridge into Jess and press start. All assay steps from protein separation, immunoprobing, detection and analysis of data are fully automated. With just 30 minutes of experimental setup and 3 hours of hands-free run time, you can be analyzing data for your next publication or grant." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Can I Get Faster Results than a Western Blot?</h4> <p> With Jess, it's pipette, run &amp; done! Simply load your sample, antibodies and reagents into the plate, insert your plate and cartridge into Jess and press start. All assay steps from protein separation, immunoprobing, detection and analysis of data are fully automated. With just 30 minutes of experimental setup and 3 hours of hands-free run time, you can be analyzing data for your next publication or grant. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-throughput.jpg" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-throughput.jpg" alt="High-Throughput Screening with Jess" title="Is Jess a Useful Method for High-Throughput Screening?" data-caption="Got a lot of samples? Jess can dramatically increase your throughput. She automates the protein separation and immunodetection of traditional Western blotting, giving you fully analyzed results in just 3 hours. Everything happens inside a capillary, and Jess precisely controls the protein separation and reagent additions and incubations, even the detection. Use Jess&#039;s fluorescent capabilities and multiplex your proteins for even higher throughput. With her 13 and 25 sample capillary cartridges you&#039;ll get the throughput you need, with minimum hands-on time." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Is Jess a Useful Method for High-Throughput Screening?</h4> <p> Got a lot of samples? Jess can dramatically increase your throughput. She automates the protein separation and immunodetection of traditional Western blotting, giving you fully analyzed results in just 3 hours. Everything happens inside a capillary, and Jess precisely controls the protein separation and reagent additions and incubations, even the detection. Use Jess's fluorescent capabilities and multiplex your proteins for even higher throughput. With her 13 and 25 sample capillary cartridges you'll get the throughput you need, with minimum hands-on time. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-quantitation.gif" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-quantitation.gif" alt="Protein Quantification with ProteinSimple’s Jess" title="Can I Quantify Proteins Like ELISA?" data-caption="Stop, analyze and wow! With Jess, protein quantitation is a breeze. At the conclusion of your run, use the lane view option to compare band intensity or dive deep for fully quantitative analysis of protein size and concentration. With a few clicks you&#039;ll be analyzing immunoassay-like standard curves and precisely quantifying your protein. Multiplexing takes your options to the max, helping you identify and differentiate relative protein expression between samples in one shot. And with in-built protein normalization, you&#039;ll have the confidence you need in your results, experiment after experiment." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Can I Quantify Proteins Like ELISA?</h4> <p> Stop, analyze and wow! With Jess, protein quantitation is a breeze. At the conclusion of your run, use the lane view option to compare band intensity or dive deep for fully quantitative analysis of protein size and concentration. With a few clicks you'll be analyzing immunoassay-like standard curves and precisely quantifying your protein. Multiplexing takes your options to the max, helping you identify and differentiate relative protein expression between samples in one shot. And with in-built protein normalization, you'll have the confidence you need in your results, experiment after experiment. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-superplex-capillary.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-superplex-capillary.png" alt="Multiplex chemiluminescent and fluorescent assays on the same sample at the same time using Jess" title="Can I Perform Multiplex Assays?" data-caption="Jess lets you Superplex, so you can do everything in one run. Like multiplexed chemiluminescent and fluorescent assays on the same sample at the same time. Or look at multiple targets and parameters simultaneously. Or get info on protein abundance, isoform and size all at once. Or add total protein without giving up a detection channel. Or detect high and low abundance proteins in the same sample, even when they have identical molecular weights. Or do chemiluminescent and fluorescent multiplexing assays in parallel just to be absolutely sure you didn&#039;t miss a protein." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Can I Perform Multiplex Assays?</h4> <p> Jess lets you Superplex, so you can do everything in one run. Like multiplexed chemiluminescent and fluorescent assays on the same sample at the same time. Or look at multiple targets and parameters simultaneously. Or get info on protein abundance, isoform and size all at once. Or add total protein without giving up a detection channel. Or detect high and low abundance proteins in the same sample, even when they have identical molecular weights. Or do chemiluminescent and fluorescent multiplexing assays in parallel just to be absolutely sure you didn't miss a protein. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-protein-normalization-export-graph.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-protein-normalization-export-graph.png" alt="Total protein detection happens in the same capillary with Simple Western" title="How Do I Normalize My Protein Expression Data?" data-caption="Total protein detection happens in the same capillary on the same sample, but runs on a separate channel from NIR so you get to keep your existing IR, NIR and chemiluminescence channels. Jess&#039; same-time detection also gives you more consistent, reliable results than traditional assays without having to stain and wash a single blot!" data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>How Do I Normalize My Protein Expression Data?</h4> <p> Total protein detection happens in the same capillary on the same sample, but runs on a separate channel from NIR so you get to keep your existing IR, NIR and chemiluminescence channels. Jess' same-time detection also gives you more consistent, reliable results than traditional assays without having to stain and wash a single blot! </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-more-data-per-sample.jpg" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-more-data-per-sample.jpg" alt="No need to strip and reprobe with RePlex on Jess" title="Can I Strip and Reprobe like a Traditional Western Blot?" data-caption="Do traditional assays use too much of your precious samples or require you to pool your samples? Jess gets you down to picogram-level sensitivity with just 3 µL of starting material. Tired of stripping and reprobing? Jess&#039;s fluorescent detection lets you maximize the data you get in one shot. Let your sample go further with Jess." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Can I Strip and Reprobe like a Traditional Western Blot?</h4> <p> Do traditional assays use too much of your precious samples or require you to pool your samples? Jess gets you down to picogram-level sensitivity with just 3 µL of starting material. Tired of stripping and reprobing? Jess's fluorescent detection lets you maximize the data you get in one shot. Let your sample go further with Jess. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-traditional-western.jpg" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_simple-western-jess-traditional-western.jpg" alt="ProteinSimple’s chemiluminescent blot imaging system" title="How Do I Image My Western Blot?" data-caption="Still doing traditional Westerns? Snap! Get the picture with her chemiluminescent blot imaging system." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>How Do I Image My Western Blot?</h4> <p> Still doing traditional Westerns? Snap! Get the picture with her chemiluminescent blot imaging system. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-650_jess-system-with-pc-1912024113810.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-650_jess-system-with-pc-1912024113810.png" alt="Multiplexed analysis of total AKT and pAKT with total protein normalization using Jess" title="Multiplexed analysis of total AKT and pAKT with total protein normalization using Jess." data-caption="(A) Lysates from Jurkat cells either untreated (-) or treated (+) with calyculin A were analyzed at 0.25 mg/mL and 0.15 mg/mL final concentrations, respectively. Each lysate was probed for total AKT with Stellar IR (green bands) and pAKT with Stellar NIR (red bands). (B) The Stellar Total Protein Assay was simultaneously performed in the same lanes shown in panel A." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Multiplexed analysis of total AKT and pAKT with total protein normalization using Jess.</h4> <p> (A) Lysates from Jurkat cells either untreated (-) or treated (+) with calyculin A were analyzed at 0.25 mg/mL and 0.15 mg/mL final concentrations, respectively. Each lysate was probed for total AKT with Stellar IR (green bands) and pAKT with Stellar NIR (red bands). (B) The Stellar Total Protein Assay was simultaneously performed in the same lanes shown in panel A. </p> </td> </tr> </article> </table> </div> <div id="how_does_work-anchor" class="nav-link static-anchor p-0" data-anchor-label="How Does Jess Automated Western Blot System Work?"></div> <div id="applications-anchor" class="nav-link static-anchor p-0" data-anchor-label="Applications"></div> <div id="products_compatible-anchor" class="nav-link static-anchor p-0" data-anchor-label="Products Compatible with Jess Automated Western Blot System"></div> <div id="specifications-anchor" class="nav-link static-anchor p-0" data-anchor-label="Specifications"></div> <div id="product-datasheet-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Documents"></div> </div> </div> <div class="tab-pane fade mx-4 py-4" id="citations-reviews--tab" data-tab-label="Citations / Reviews (1) / Testimonials (32)" role="tabpanel" aria-labelledby="citations-reviews--tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#reviews"> Reviews </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#testimonials"> Testimonials </a> </li> </div> </div> <div id="citations"> <div class="pp_filter_list border-bottom mb-4 pb-3"> <h2>Citations for Jess Automated Western Blot System</h2> <p><a href="/resources/instrument-citations?instrument-platform=simple%20western" rel="nofollow noopener noreferrer" target="_blank">View all citations for the Simple Western platform.</a><br> Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. </p> </div> </div> <div class="mt-4" id="reviews"> <section id="reviews-tab-content-placeholder" class="tab-content-placeholder" data-id="reviews" data-product_code="004-650"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> </div> <div class="testimonials" id="testimonials"><article class="product-data-wrapper"> <div class="pp_filter_list mt-4 pt-4 border-bottom mb-4 pb-3"> <h2>Testimonials from your Peers for Simple Western</h2> <div class="testimonials_showing pp_filters_showing row col-md-12 mb-2"> <span class="less">Showing&nbsp; <span class="testimonials_show_min font-weight-bold">1</span> <span class="font-weight-bold">-</span> <span class="testimonials_show_max font-weight-bold">5</span> of <span class="testimonials_count font-weight-bold">32</span> testimonials </span> <span class="more font-weight-bold">Showing All</span> </div> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/headshots/tatiana-rosenstock-headshotv2.png" alt="Tatiana Rosenstock, Principal Scientist, Sygnature Discovery" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Jess is faster and more reliable in terms of quantification and allows us to use low sample volumes, for example, difficult-to-obtain samples such as iPSC-derived neurons."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/tatiana-moves-closer-to-treating-neurodegenerative-diseases-with-jess">Tatiana Moves Closer to Treating Neurodegenerative Diseases With Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Tatiana Rosado Rosenstock, </span> Principal Scientist, Sygnature Discovery </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/headshots/fyp-Chris-LaBreck-200x200.png" alt="Chris LaBreck, Ph.D., Senior Product Scientist, Cell Signaling Technology" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-cst-antibody-validation">Translating Traditional Western Blots to Simple Western is Fast and Easy</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Chris LaBreck, Ph.D., </span> Senior Product Scientist, Cell Signaling Technology (CST) </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/headshots/fpy-headshot-laura-martins-200x200.png" alt="Laura Martins Ph.D." loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Simple Western™ allows us to run three-hour assays, which would usually take us two days with traditional Western blots."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/advancing-cell-and-gene-therapies-with-simple-western">Advancing Cell and Gene Therapies with Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Laura Martins, Ph.D., </span> Early Development Scientist, Oxford Biomedica </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/fyp_john_jess.png" alt="John Blackwood PhD Head of Biology at Samsara Therapeutics Headshot" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease"</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/restoring-autophagy-reversing-disease-with-jess">Restoring Autophagy, Reversing Disease with Jess </a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> John K. Blackwood PhD, </span> Head of Biology, and Jakub Stefaniak PhD, Samsara Therapeutics </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/fyp_mario_abby.png" alt="FYP Abby Competition Mario Tile" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/musculoskeletal-disease-researchers-utilitze-abby-for-protein-analysis">Accelerate Osteoarthritis Research Studies with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Mario Rothbauer, </span> Postdoc Researcher, Department of Orthopedics and Trauma Surgery, Medical University of Vienna </p> </section> </div> </div> </article> <span id="more-testimonials" style="display:none"> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-harte.jpg" alt="Dr Michael Harte tile" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. The appropriate control and low variability between plates using Simple Western™ means we can complete our studies in weeks rather than months."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/utilizing-simple-western-to-understand-neuronal-markers-schizophrenia">Utilizing Simple Western to Understand Neuronal Markers in Schizophrenia</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Michael Harte, </span> Senior Lecturer in Drug Action, Neuroscience, University of Manchester </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-noelle-anastasio.png" alt="Noelle Anastasio Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"One of the best advantages of using Wes is that it saves a lot of time, both in terms of hands-on time and in time to completion of the experiment. We were spending more than 4 hours of hands-on time per Western blot with traditional methods, and we can set up a run on the Wes in about 45 minutes. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-changes-brain-receptor-research-game">Noelle Changes Her Brain Receptor Research Game with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Noelle Anastasio, Ph.D., </span> Assistant Professor, Department of Pharmacology and Toxicology, University of Texas Medical Branch </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-mary-howell.png" alt="Elizabeth Rick uses Luminex porcine assays to perform traumatic brain injury biomarker research" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. With Wes, we can do multiple assays with one sample collection."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-ups-data-point-count-on-laser-capture-microdissection-samples">Mary Ups Her Data Point Count on Laser Capture Microdissection Samples with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Mary Howell, </span> Laboratory Coordinator, Department of Internal Medicine, East Tennessee State University </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-miranda.png" alt="Miranda Orr Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. This technology has made it possible for small research labs to compete with the pace that research is conducted in large labs or companies."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-sets-a-new-pace-for-alzheimers-research">Miranda Sets a New Pace for Alzheimer&#039;s Research with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Miranda Orr, Ph.D., </span> Postdoctoral Fellow, University of Texas Health Science Center </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-brian-perrino.png" alt="Brian Perrino Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"We had been trying to study receptor proteins with traditional Westerns but it was frustrating—we just could not get it. A few months with Wes and we've already had way more success than we had in three years."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-cuts-time-to-results-and-solves-membrane-protein-challenges">Brian Cuts Time to Results and Solves His Membrane Protein Challenges with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Brian Perrino, Ph.D., </span> Associate Professor, Department of Physiology and Cell Biology, University of Nevada School of Medicine </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-jamie-boisvenue.png" alt="Jamie Boisvenue Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The traditional blotting method can use up to 300 pieces of plastic and [Simple Western] uses two. What's even better is that [Simple Western] can run up to 40 proteins in three days. It would take about a week to run 40 proteins using Western blotting."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-keeps-things-greener">Jamie Keeps Things Greener with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Jamie Boisvenue, </span> Cardiovascular Research Technician, Department of Pediatrics, University of Alberta Faculty of Medicine &amp; Dentistry </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-mary-g-johlfs.png" alt="Mary G. Johlfs Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-Iα and PKG-Iβ and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/nanopro-links-between-obesity-and-cancer">Distinguish PKG Antibody Isoforms with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Mary G. Johlfs, M.S., </span> Director of Research Operations/Scientist, Roseman University of Health Sciences </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-emma-wilson.png" alt="Emma Wilson" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Wes gives me more throughput so I can get more replicates and trust my results a little bit more, and I can trust them straight away rather than having to do a lot of repeats. And obviously I like getting results the same day rather than having to wait two days"</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-better-outcomes-for-osteoarthritis-patients">Emma Gets to Better Outcomes for Osteoarthritis Patients with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Emma Wilson (née Humphrey), Ph.D., </span> Lecturer in Molecular Biology, Institute of Medicine, University of Chester </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-uconn-Kazuyam.png" alt="Kazuya Machida thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The no-gel, no-transfer, no-membrane features are beneficial for the reproducibility of the assay. I really like this system over traditional Western."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/far-western-blotting-detect-protein-phosphorylation-with-simple-western-wes">Far Western Blot With Simple Western To Detect Protein Phosphorylation</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Kazuya Machida, M.D., Ph.D., </span> Associate Professor, Department of Genetics and Genome Sciences, UConn Health </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-melyssa-bratton.png" alt="Melyssa Bratton thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"We’ve been able to increase productivity dramatically with Wes. The results are much better too, given that it removes the blotting step all together."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-speeds-up-the-core">Melyssa Speeds Up the Core with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Melyssa Bratton, Ph.D., </span> Manager of the Cell, Molecular, and Biostatistics Core, Xavier University of Louisiana </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-jane.png" alt="Dr Jane Gray Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"[Simple Western] is easy to use, convenient, and gives answers quickly. The throughput and system automation is a definite advantage."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-gets-fast-answers-for-cancer-research">Make Quick Advances in Cancer Research with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Jane Gray, Ph.D., </span> Head of Research Instrumentation, Cancer Research UK Cambridge Institute </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-debbi-hicks.jpg" alt="Debbi Hicks Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Some of the proteins we wanted to detect are low in abundance and it’s really hard to get a good amount of protein for traditional Western blot. The low protein concentrations required by [Simple Western] makes it easier to save precious samples ensuring protein detection."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-detecting-low-abundance-proteins-in-precious-pediatric-cancer-samples">Detecting Low Abundant Proteins in Precious Pediatric Cancer Samples</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Debbie Hicks, Ph.D., </span> Faculty Fellow, Wolfson Childhood Cancer Research Centre, Northern Institute for Cancer Research, Newcastle University </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-irene-tobias.jpg" alt="Irene Tobias Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Wes allows us to measure cell signaling proteins from a single muscle fiber segment by lowering our limit of detection nearly ~20-fold from Western blotting. It also allows for much more precise, reproducible, and faster results to be generated, in addition to far greater ease of operation, analysis, and waste disposal."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-lower-limit-detection-advances-understanding-of-metabolic-signaling-in-human-muscle">Lower Limit of Detection Advances Understanding of Metabolic Signaling in Human Muscle</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Irene Tobias, Ph.D., </span> Postdoctoral Researcher, CSU Fullerton </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-y-senis.jpg" alt="Yotis Senis Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"[Wes] Provides quantitative data, saves time and uses far less sample."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-quantifying-protein-phosphorylation-in-platelets">Quantifying Protein Tyrosine Phosphatase in Platelets</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Yotis Senis, Ph.D., </span> Professor and Chair, University of Birmingham, UK </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-krishna.png" alt="Krishna C. Yalla" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I was able to screen various antibodies in a single run within half a day. This had previously taken weeks and months using traditional Western blotting methods"</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-pharmacodynamic-biomarker-discovery">Wes Zips Through Pharmacodynamic Biomarker Discovery Projects at the University of Glasgow</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Krishna C. Yalla, Ph.D., </span> Postdoctoral Research Scientist, Institute of Cancer Sciences, University of Glasgow </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-j-voutila.png" alt="Jon Voutila Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I can run immunoassays on Wes for an entire animal experiment in a single day. This is something I wouldn’t even try with traditional Western blots."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-validates-RNA-based-metabolic-therapies-potential">Wes Validates the Potential of RNA-based Metabolic Therapies At MiNA Therapeutics</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Jon Voutila, Ph.D., </span> Senior Research Scientist, MiNA Therapeutics, London, UK </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-ferrari.png" alt="Dr Ferrari Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Simple Western allowed me to screen quickly lots of samples with different antibodies while eliminating the manual washing and incubation steps."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/jess-simple-western-coronavirus-vaccine">Simple Western and the Race to Develop a Vaccine for the COVID-19 Coronavirus</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Matteo Ferrari, </span> Postdoc, DIOSynVax, Department of Veterinary Medicine, Cambridge, United Kingdom </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-volta.png" alt="Dr. Mattia Volta thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"It’s had a significant impact on our research... we get double the amount of data in a third of the time."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/researching-origins-of-parkinsons-disease-with-simple-western">Rebel without a Cause - Searching for the Origins of Parkinson&#039;s Disease with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Mattia Volta, </span> Senior Researcher, EURAC Research </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-rachel-doidge.png" alt="Rachel Doidge thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/protac-drug-discovery-jess">PROTAC Studies Take Off in Drug Discovery, and Jess is on Board</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Rachel Doidge Ph.D, </span> Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-tonmoy-sarkar.png" alt="Tonmoy Sarkar Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The quantitative assays and short run times on Jess enable us to generate data faster and be more confident in our results."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/jess-screens-new-chemical-entities-at-tcg-lifesciences">Jess Breaks New Quantitative Ground in New Chemical Entity Screening at TCG Lifesciences</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Tonmoy Sarkar, Ph.D., </span> Senior Research Scientist, TCG Lifesciences </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-kamarudin.png" alt="Muhumad Noor Alfarizal Kamarudin thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Jess allows me to reduce the preparation time, save on reagents, samples, and antibodies, while allowing easier optimization of target proteins/antibodies. Jess’s ability to detect multiple proteins per sample further saves time compared to conventional Western blot."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/jess-finds-novel-therapeutics-for-glioma">Conducting Novel Therapeutics Research of Human Gliomas with Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Muhumad Noor Alfarizal Kamarudin, </span> Research Fellow, Brain Research Institute Monash Sunway (BRIMS) </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-nicolas-g-bazan.png" alt="Nicolas Bazan thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"It saves so much time! It takes away so much of the pipetting and manipulation error. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/unraveling-neurodegenerative-diseases-with-jess">Unraveling Neurodegenerative Diseases with Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Nicolas G. Bazan, MD, PhD, </span> Director of Neuroscience Center of Excellence, School of Medicine, Louisiana State University Health New Orleans, New Orleans </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-joe-klebba.png" alt="Joe Klebba Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"By switching my assays over to Simple Western, I am able to increase my throughput, allowing for full dose responses to be generated in each experiment. Utilizing the RePlex module even further increases this throughput as I often probe for 5-6 proteins per capillary in a single run including phosphorylated/total protein pairs in the same lane."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/targeting-once-inaccessible-phospho-proteins-with-replex">Detect Phosphorylation of Proteins with RePlex</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Joseph Klebba, Ph.D., </span> Scientist, Vividion Therapeutics </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-elena-navarro-guerrero.jpg" alt="Elena Navarro-Guerrero" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I used Simple Western because the protein samples I had were small. We only had a small number of [iPSC-derived macrophages] because the cell line does not proliferate."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/elena-unlocks-immune-cell-potential-simple-western-crispr-cas9">Elena Unlocks Immune Cell Potential with Simple Western and CRISPR/Cas9</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Elena Navarro-Guerrero, Ph.D., </span> Senior Postdoctoral Researcher. Nuffield Department of Medicine, Target Discovery Institute </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-muhammad-dain-yazid.png" alt="Muhammad Dain Yazid" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Protein analysis using [Simple Western] can be performed faster, eliminating tedious work and saving time."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/muhammad-simple-western-chart-signaling-pathways-stem-cells">Muhammad Uses Simple Western to Chart Stem Cell Signaling Pathways</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Muhammad Dain Yazid, Ph.D., </span> Centre for Tissue Engineering &amp; Regenerative Medicine, University Kebangsaan Malaysia (UKM) Medical Centre </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-kristine-nolling-jensen.png" alt="Kirstine Nolling Jensen, PhD Student" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Jess is a user-friendly, multi-purpose platform that gives me the possibility to perform Western blot analysis on precious samples, as well as quantifying proteins expressed in the cells at the same time."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/kirstine-nolling-jensen-Investigating-resolution-inflammation-jess">Investigating Resolution of Inflammation with Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Kirstine Nolling Jensen, PhD Student, </span> Department of Immunology, Landspitali, the National University Hospital Iceland </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-nageswara-karolinska.png" alt="Nageswara Rao Boggavarapu, PhD." loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Our lab came across ProteinSimple’s Simple Western platforms in 2014 and was surprised by the sensitivity and specificity of the technology, as well as its minimal usage of consumables."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/nageswara-complexities-of-endometrial-receptivity-with-simple-western">Delving into the Complexities of Endometrial Receptivity with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Nageswara Rao Boggavarapu, PhD, </span> Assistant Professor, Women’s and Children’s Health, Karolinska Institute, Stockholm, Sweden </p> </section> </div> </div> </article> </span> <div id="testimonial_counts" data-max="32" data-min="5"> <div class="testimonials_showing pp_filters_showing row col-md-12 mb-2"> <span class="less">Showing&nbsp; <span class="testimonials_show_min font-weight-bold">1</span> <span class="font-weight-bold">-</span> <span class="testimonials_show_max font-weight-bold">5</span> of <span class="testimonials_count font-weight-bold">32</span> testimonials </span> <span class="more font-weight-bold">Showing All</span> </div> </div> <div class="text-center"> <button id="testimonials-view-more" class="btn-secondary">View More</button> </div> </div> </article> </div> </div> </div> </div> <div><div id="accordion" class="border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3">More Information About Jess Automated Western Blot System</h3> </div> <div class="vtab-section" data-section="How Does Jess Automated Western Blot System Work?" id="how-does-jess-automated-western-blot-system-work"> <div class="card-header rounded-top"> <a id="how-does-jess-automated-western-blot-system-work-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-5781592502255" aria-expanded="false" aria-controls="collapse_region-5781592502255"> <h5 class="mb-0"> How Does Jess Automated Western Blot System Work? </h5> </a> </div> <div id="collapse_region-5781592502255" class="collapse " aria-labelledby="heading_region-5781592502255" data-parent="#accordion"> <div class="card-body"> <div id="how-does-jess-automated-western-blot-system-work-vtab" data-tab-label="How Does Jess Automated Western Blot System Work?" role="tabpanel" aria-labelledby="how-does-jess-automated-western-blot-system-work-vtab-tab"><div id="instrument_how_work_content"> <div class="paragraph paragraph--type--product-page-content paragraph--view-mode--full"> <div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="B1LHY4Hz75E" params="rel=0" style="background-image: url('https://img.youtube.com/vi/B1LHY4Hz75E/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/B1LHY4Hz75E?autoplay=1" class="lty-playbtn" title="How to Set-Up Jess Training Kits"> <span class="lyt-visually-hidden">How to Set-Up Jess Training Kits</span> </a> </lite-youtube> </div> </article> </div> </div> <div class="media-caption"> <div><br><a href="/resources/protocols/jess-abby-wes-protocols">View Jess Protocols</a></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p><a href="/instruments/simple-western">Simple Western</a><sup>™</sup> assays on Jess<sup>™</sup> are automated, capillary-based immunoassays that solve many of the challenges that come with traditional Westerns. All assay steps from protein separation, immunoprobing, detection, and analysis are fully automated. The variability in the manual processes that impact reproducibility, quantitation, time to result, and overall data reliability is eliminated. Best of all, the transition is simple—the same <a href="/resources/simple-western-antibody-database">antibodies</a> you use for Western blotting protocols can be used with Simple Western assays.<br> Your sample, separation matrix, stacking matrix, antibodies, and reagents are loaded automatically from a specially designed plate. Jess begins by aspirating the separation matrix and then the stacking matrix into each capillary. Next, your sample is loaded, and capillaries are lowered to make contact with running buffer. Voltage is applied to enable separation by molecular weight. Once the separation is complete, UV light immobilizes the proteins to the capillary wall. With proteins now immobilized and the matrix cleared of the capillary, Jess starts the immunoprobing process. In just 3 hours you'll have quantitative, <a href="/instruments/simple-western/simple-western-assays-utilizing-size-based-separation">size-based data</a> ready for analysis.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/p/instruments/simple-western/request-quote" class="btn btn-primary" type="link"> Request Pricing </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><blockquote> <p><sup>Jess is a trademark of ProteinSimple, Corp.</sup></p> </blockquote></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="vtab-section" data-section="Applications" id="applications"> <div class="card-header rounded-top"> <a id="applications-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-4951592502294" aria-expanded="false" aria-controls="collapse_region-4951592502294"> <h5 class="mb-0"> Applications </h5> </a> </div> <div id="collapse_region-4951592502294" class="collapse " aria-labelledby="heading_region-4951592502294" data-parent="#accordion"> <div class="card-body"> <div id="applications-vtab" data-tab-label="Applications" role="tabpanel" aria-labelledby="applications-vtab-tab"><div id="applications_wrapper"> <div class="paragraph paragraph--type--product-page-content paragraph--view-mode--full"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Jess Applications</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cdx-quantidex-cancer-cell-350x350.png" alt="general cancer cell b" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > Immuno-Oncology </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/instruments/simple-western/applications/advanced-western-blotting-solutions-for-cell-signaling" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/immune-profiling-teaser-fore-green-480x307.png" alt="green immune cell sending out cytokines" loading="lazy" > </div> </a> <a class="card_title_link" href="/instruments/simple-western/applications/advanced-western-blotting-solutions-for-cell-signaling" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/instruments/simple-western/applications/advanced-western-blotting-solutions-for-cell-signaling" > Cell Signaling Pathways </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/tcell-cars-foreground-525x525.png" alt="T Cells with Cars" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions" > Cell &amp; Gene Therapy </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-targeted-protein-degradation" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/targeted-protein-degradation-frontimg.png" alt="PROTAC degrader molecule in complex with an E3 ligase and the target protein" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-solutions-for-targeted-protein-degradation" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-targeted-protein-degradation" > Targeted Protein Degradation </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/extracellular-vesicle-protein-analysis-with-jess" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/asu-diagnostics-exo-cell-hero-480x480.png" alt="exosome" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/extracellular-vesicle-protein-analysis-with-jess" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/extracellular-vesicle-protein-analysis-with-jess" > Extracellular Vesicles </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/high-throughput-pharmacokinetic-analysis-with-simple-western" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/bt-antibodies-hero.png" alt="Antibody Hero Cell" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/high-throughput-pharmacokinetic-analysis-with-simple-western" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/high-throughput-pharmacokinetic-analysis-with-simple-western" > Pharmacokinetic Analysis </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-vaccine-development" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Cell-Characterization-Front.png" alt="Vials for bioprocessing solutions" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-solutions-for-vaccine-development" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-vaccine-development" > Vaccine Development </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-neuroscience" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/nfl-hero-dopaminergic2.png" alt="3d remendering of dopaminergic cell for neuroscience research" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-solutions-for-neuroscience" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-neuroscience" > Neuroscience </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/bioprocess-contaminant-detection-using-simple-western" > <div 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href="/resources/instrument-applications/advanced-western-blotting-applications-for-sars-cov-2-research" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/bt-covid-research-tool-hero-img.png" alt="Covid Cell Research Tool Hero Image" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-applications-for-sars-cov-2-research" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-applications-for-sars-cov-2-research" > SARS-CoV-2 Research </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/sars-cov-2-multi-antigen-serology-module-simple-western" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dark-purple-teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/sars-cov2-teasercard-cell-v2.png" alt="Antibodies for SARS-CoV-2 Detection" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/sars-cov-2-multi-antigen-serology-module-simple-western" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/sars-cov-2-multi-antigen-serology-module-simple-western" > Multi-Antigen Serology </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="vtab-section" data-section="Products Compatible with Jess Automated Western Blot System" id="products-compatible-with-jess-automated-western-blot-system"> <div class="card-header rounded-top"> <a id="products-compatible-with-jess-automated-western-blot-system-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-7641628092667" aria-expanded="false" aria-controls="collapse_region-7641628092667"> <h5 class="mb-0"> Products Compatible with Jess Automated Western Blot System </h5> </a> </div> <div id="collapse_region-7641628092667" class="collapse " aria-labelledby="heading_region-7641628092667" data-parent="#accordion"> <div class="card-body"> <div id="products-compatible-with-jess-automated-western-blot-system-vtab" data-tab-label="Products Compatible with Jess Automated Western Blot System" role="tabpanel" aria-labelledby="products-compatible-with-jess-automated-western-blot-system-vtab-tab"><div id="products_compatible_with_instrument"><article class="product-data-wrapper"> <ul class="compatible_instruments list-unstyled m-0"> <li class="py-2"><a href="/resources/simple-western-antibody-database">Simple Western Antibody Database</a></li> <li class="py-2"><a href="/search?keywords=Simple%20Western%20Assay%20Modules&amp;sub_category=Simple%20Western%20Assay%20Modules&amp;compatible_instrument=Jess&amp;view=condensed">Simple Western Assay Modules</a></li> <li class="py-2"><a href="/search?keywords=Simple%20Western%20Consumables&amp;sub_category=Simple%20Western%20Consumables&amp;compatible_instrument=Jess&amp;view=condensed">Simple Western Consumables</a></li> <li class="py-2"><a href="/search?keywords=Simple%20Western%20Consumables%20for%20Lot%20Controlled%20Orders&amp;sub_category=Simple%20Western%20Consumables%20for%20Lot%20Controlled%20Orders&amp;compatible_instrument=Jess&amp;view=condensed">Simple Western Consumables for Lot Controlled Orders</a></li> <li class="py-2"><a href="/search?keywords=Simple%20Western%20Individual%20Charge%20Reagents&amp;sub_category=Simple%20Western%20Individual%20Charge%20Reagents&amp;compatible_instrument=Jess&amp;view=condensed">Simple Western Individual Charge Reagents</a></li> <li class="py-2"><a href="/resources/simple-western-kit-builder">Simple Western Kit Builder</a></li> <li class="py-2"><a href="/search?keywords=simple%20western&amp;compatible_instrument=Jess&amp;view=condensed">View All Products Compatible with Jess</a></li> </ul> </article> </div> </div> </div> </div> </div> <div class="vtab-section" data-section="Specifications" id="specifications"> <div class="card-header rounded-top"> <a id="specifications-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-9701592502350" aria-expanded="false" aria-controls="collapse_region-9701592502350"> <h5 class="mb-0"> Specifications </h5> </a> </div> <div id="collapse_region-9701592502350" class="collapse " aria-labelledby="heading_region-9701592502350" data-parent="#accordion"> <div class="card-body"> <div id="specifications-vtab" data-tab-label="Specifications" role="tabpanel" aria-labelledby="specifications-vtab-tab"><div id="instrument_specifications"><table class="tablesaw tablesaw-stack app_table my-3 table-bordered" data-tablesaw-mode="stack"> <thead class="ds_table_head bg-light-gray"> <th class="ds_table_head_cell w-25 p-3">Description</th> <th class="ds_table_head_cell w-25 p-3">Total Protein Specification</th> <th class="ds_table_head_cell w-25 p-3">Chemiluminescence and Stellar Fluorescence Specification</th> <th class="ds_table_head_cell w-25 p-3">Classic Fluorescence Specification</th> <th class="ds_table_head_cell w-25 p-3">Protein Normalization Specification</th> </thead> <tbody class="bg-white"> <tr class="ds_table_row"> <td>Sample required</td> <td colspan="1" class="text-left"> 0.3-1.2 µg </td> <td colspan="1" class="text-left"> 0.6-1.2 µg </td> <td colspan="1" class="text-left"> 2-4 µg </td> <td colspan="1" class="text-left"> 0.6-4 µg </td> </tr> <tr class="ds_table_row"> <td>Volume required</td> <td colspan="4" class="text-center"> 3 µL/well </td> </tr> <tr class="ds_table_row"> <td>Size range</td> <td colspan="4" class="text-center"> Molecular weight (MW) ladder ranges from 2-440 kDa </td> </tr> <tr class="ds_table_row"> <td>Sizing CV</td> <td colspan="4" class="text-center"> <10% </td> </tr> <tr class="ds_table_row"> <td>Intra-assay CV</td> <td colspan="3" class="text-center"> <15% </td> <td colspan="1" class="text-left"> <20% </td> </tr> <tr class="ds_table_row"> <td>Inter-assay CV</td> <td colspan="1" class="text-left"> <20%** </td> <td colspan="1" class="text-left"> <20%* </td> <td colspan="1" class="text-left"> <20% </td> <td colspan="1" class="text-left"> <20% </td> </tr> <tr class="ds_table_row"> <td>Resolution (± percent difference in MW)</td> <td colspan="4" class="text-center"> ± 15-20% for MW <20 kDA<br />± 10% for MW >20 kDa </td> </tr> <tr class="ds_table_row"> <td>Quantitation CV</td> <td colspan="3" class="text-center"> <20% (total protein, chemiluminescence and fluorescence) </td> <td colspan="1" class="text-left"> N/A </td> </tr> <tr class="ds_table_row"> <td>Dynamic range</td> <td colspan="1" class="text-left"> 2-3 logs </td> <td colspan="2" class="text-center"> 3-4 logs </td> <td colspan="1" class="text-left"> 1 logs </td> </tr> <tr class="ds_table_row"> <td>Sensitivity</td> <td colspan="1" class="text-left"> ng </td> <td colspan="1" class="text-left"> Low pg </td> <td colspan="1" class="text-left"> High pg </td> <td colspan="1" class="text-left"> ng </td> </tr> <tr class="ds_table_row"> <td>Capillary</td> <td colspan="4" class="text-center"> 5 cm, 100 µm, 400 nL </td> </tr> <tr class="ds_table_row"> <td>Runtime</td> <td colspan="3" class="text-center"> <3 hours </td> <td colspan="1" class="text-left"> <4 hours with immunoassay </td> </tr> <tr class="ds_table_row"> <td>Samples per run</td> <td colspan="4" class="text-center"> 13 or 25 </td> </tr> <tr class="ds_table_row"> <td>Weight</td> <td colspan="4" class="text-center"> 23 kg </td> </tr> <tr class="ds_table_row"> <td>Dimensions (closed)</td> <td colspan="4" class="text-center"> 0.36 m H X 0.3 m W X 0.57 m D </td> </tr> <tr class="ds_table_row"> <td>Dimensions (open)</td> <td colspan="4" class="text-center"> 0.36 m H x 0.53 m W x 0.57 m D </td> </tr> <tr class="ds_table_row"> <td>Power</td> <td colspan="4" class="text-center"> US/CAN 120 V AC, 60 hz, 4.2 amps<br />Europe 240 AC, 50 Hz, 2.1 amps<br />Japan 100 AC, 50/60 Hz, 5.0 amps </td> </tr> <tr class="ds_table_row"> <td>Operating temperature</td> <td colspan="4" class="text-center"> 18-24 °C </td> </tr> <tr class="ds_table_row"> <td>Operating humidity</td> <td colspan="4" class="text-center"> 20-60% relative, non-condensing </td> </tr> </tbody> </table> <div class="mt-2"> <div><small class="my-1"> * Inter-assay CV is calculated with normalization to the system control protein </small> </div> <div><small class="my-1"> ** Percent peak area </small> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div id="product-documents"> <div id="accordion-arena-documents" class="accordion border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3">Product Documents for Jess Automated Western Blot System</h3> </div> <div class="accordion-item vtab-section"> <div class="card-header rounded-top" id="document-heading1"> <a class="collapsed arena-document-title" id="product-documents-link" data-toggle="collapse" data-target="#document-collapse1" aria-expanded="false" aria-controls="document-collapse1"> <h5>Brochure</h5> </a> </div> <div id="document-collapse1" class="collapse" 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