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class="external-links-container"><ul class="profile-links new-profile js-UserInfo-social"><li class="profile-profiles js-social-profiles-container"><i class="fa fa-spin fa-spinner"></i></li></ul></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Basile Michaelidis</h3></div><div class="js-work-strip profile--work_container" data-work-id="77626234"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77626234/Heat_hardening_enhances_mitochondrial_potential_for_respiration_and_oxidative_defence_capacity_in_the_mantle_of_thermally_stressed_Mytilus_galloprovincialis"><img alt="Research paper thumbnail of Heat hardening enhances mitochondrial potential for respiration and oxidative defence capacity in the mantle of thermally stressed Mytilus galloprovincialis" class="work-thumbnail" src="https://attachments.academia-assets.com/84945231/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77626234/Heat_hardening_enhances_mitochondrial_potential_for_respiration_and_oxidative_defence_capacity_in_the_mantle_of_thermally_stressed_Mytilus_galloprovincialis">Heat hardening enhances mitochondrial potential for respiration and oxidative defence capacity in the mantle of thermally stressed Mytilus galloprovincialis</a></div><div class="wp-workCard_item"><span>Scientific Reports</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Ectotherms are exposed to a range of environmental temperatures and may face extremes beyond thei...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Ectotherms are exposed to a range of environmental temperatures and may face extremes beyond their upper thermal limits. Such temperature extremes can stimulate aerobic metabolism toward its maximum, a decline in aerobic substrate oxidation, and a parallel increase of anaerobic metabolism, combined with ROS generation and oxidative stress. Under these stressful conditions, marine organisms recruit several defensive strategies for their maintenance and survival. However, thermal tolerance of ectothermic organisms may be increased after a brief exposure to sub-lethal temperatures, a process known as &quot;hardening&quot;. In our study, we examined the ability of M. galloprovincialis to increase its thermal tolerance under the effect of elevated temperatures (24, 26 and 28聽掳C) through the &quot;hardening&quot; process. Our results demonstrate that this process can increase the heat tolerance and antioxidant defense of heat hardened mussels through more efficient ETS activity when expos...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c59e23830963e45dc116167f23bd18c9" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":84945231,"asset_id":77626234,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/84945231/download_file?st=MTczMzk0ODQ2Nyw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77626234"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77626234"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77626234; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77626234]").text(description); $(".js-view-count[data-work-id=77626234]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77626234; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77626234']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77626234, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c59e23830963e45dc116167f23bd18c9" } } $('.js-work-strip[data-work-id=77626234]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77626234,"title":"Heat hardening enhances mitochondrial potential for respiration and oxidative defence capacity in the mantle of thermally stressed Mytilus galloprovincialis","translated_title":"","metadata":{"abstract":"Ectotherms are exposed to a range of environmental temperatures and may face extremes beyond their upper thermal limits. 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Could systematic monitoring and early warning prevent negative impacts A review focused on Vistonikos gulf, North Aegean Sea</a></div><div class="wp-workCard_item"><span>International Journal of Agricultural Resources, Governance and Ecology</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892207"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892207"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892207; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892207]").text(description); $(".js-view-count[data-work-id=53892207]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892207; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892207']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892207, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892207]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892207,"title":"The effect of seawater physical parameters in bivalve farming. 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The devastating mortality of the species has turned the interest of the research towards the causes of these events. After the haplosporidan infestation and the infection by Mycobacterium sp., new emerging pathogens have arisen based on the latest research. In the present study, a metagenomic approach of 16S rRNA next generation sequencing (NGS) was applied in order to assess the bacterial diversity within the digestive gland of diseased individuals as well as to carry out geographical correlations among the biodiversity of microbiome in the endangered species Pinna nobilis. The specimens originated from the mortalities occurred in 2019 in the region of Greece. Together with other bacterial genera, the results confirmed the presence of Vibrio spp., assuming synergistic effects in the mortality events of the species. Alongside with t...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="16a4e594b4e0812e65e57541575d0d12" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519394,"asset_id":53892204,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519394/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892204"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892204"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892204; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892204]").text(description); $(".js-view-count[data-work-id=53892204]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892204; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892204']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892204, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "16a4e594b4e0812e65e57541575d0d12" } } $('.js-work-strip[data-work-id=53892204]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892204,"title":"Gut Symbiotic Microbial Communities in the IUCN Critically Endangered Pinna nobilis Suffering from Mass Mortalities, Revealed by 16S rRNA Amplicon NGS","translated_title":"","metadata":{"abstract":"Mass mortality events due to disease outbreaks have recently affected almost every healthy population of fan mussel, Pinna nobilis in Mediterranean Sea. 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status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892189/First_insights_towards_the_population_genetic_structure_and_the_phylogeographic_status_of_the_horse_mussel_Modiolus_barbatus_from_the_eastern_Mediterranean">First insights towards the population genetic structure and the phylogeographic status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean</a></div><div class="wp-workCard_item"><span>Journal of the Marine Biological Association of the United Kingdom</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The horse mussel Modiolus barbatus is a marine benthic bivalve, distributed mainly in the Mediter...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The horse mussel Modiolus barbatus is a marine benthic bivalve, distributed mainly in the Mediterranean basin, that constitutes a fishery product of high economic importance and a promising candidate for aquaculture. The current study provides the first insights regarding the genetic profile of M. barbatus populations from the eastern Mediterranean, by analysis of a partial segment of the mitochondrial COI gene in individuals collected from five sampling localities within the Aegean Sea. To the best of our knowledge, the derived haplotypes represent the first DNA barcodes of M. barbatus from the entire Mediterranean region. Maximum likelihood phylogenetic analysis revealed that M. barbatus from the North Sea and M. barbatus from the eastern Mediterranean may not belong to the same species and as a consequence, there might be three species of the genus Modiolus in Europe. On the other hand, eastern Mediterranean M. barbatus haplotypes were found to be more closely related to the Asia...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892189"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892189"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892189; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892189]").text(description); $(".js-view-count[data-work-id=53892189]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892189; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892189']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892189, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892189]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892189,"title":"First insights towards the population genetic structure and the phylogeographic status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean","translated_title":"","metadata":{"abstract":"The horse mussel Modiolus barbatus is a marine benthic bivalve, distributed mainly in the Mediterranean basin, that constitutes a fishery product of high economic importance and a promising candidate for aquaculture. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892184"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892184/Seasonal_Changes_in_Metabolism_and_Cellular_Stress_Phenomena_in_the_Gilthead_Sea_Bream_Sparus_aurata_"><img alt="Research paper thumbnail of Seasonal Changes in Metabolism and Cellular Stress Phenomena in the Gilthead Sea Bream (Sparus aurata)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892184/Seasonal_Changes_in_Metabolism_and_Cellular_Stress_Phenomena_in_the_Gilthead_Sea_Bream_Sparus_aurata_">Seasonal Changes in Metabolism and Cellular Stress Phenomena in the Gilthead Sea Bream (Sparus aurata)</a></div><div class="wp-workCard_item"><span>Physiological and biochemical zoology : PBZ</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Seasonal temperature changes may take organisms to the upper and lower limit of their thermal ran...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Seasonal temperature changes may take organisms to the upper and lower limit of their thermal range, with respective variations in their biochemical and metabolic profile. To elucidate these traits, we investigated metabolic and antioxidant patterns in tissues of sea bream Sparus aurata during seasonal acclimatization for 1 yr in the field. Metabolic patterns were assessed by determining lactate dehydrogenase, citrate synthase, and 尾-hydroxyacyl CoA dehydrogenase activities, their kinetic properties and plasma levels of glucose, lactate, and triglycerides and tissue succinate levels. Oxidative stress was assessed by determining antioxidant enzymes superoxide dismutase, catalase, and glutathione reductase activities and levels of thiobarbituric acid reactive substances. Xanthine oxidase (XO) activity was determined as another source of reactive oxygen species (ROS) production. Furthermore, we studied the antiapoptotic protein indicator Bcl-2 and the apoptotic protein indicators Bax, ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892184"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892184"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892184; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892184]").text(description); $(".js-view-count[data-work-id=53892184]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892184; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892184']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892184, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892184]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892184,"title":"Seasonal Changes in Metabolism and Cellular Stress Phenomena in the Gilthead Sea Bream (Sparus aurata)","translated_title":"","metadata":{"abstract":"Seasonal temperature changes may take organisms to the upper and lower limit of their thermal range, with respective variations in their biochemical and metabolic profile. 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A major constraint to application of physiological knowledge for conservation of marine fishes is the limited knowledge base; international collaboration is needed to study the environmental physiology of a wider range of species. Multifactorial field and laboratory studies on biomarkers hold promise to relate ecophysiology directly to habitat quality and population status. The &#39;Fry paradigm&#39; could have broad applications for conservation physiology research if it provides a universal mechanism to link physiological function with ecological performance and population dynamics of fishes, through effects of abiotic conditions on aerobic metabolic scope. The available data indicate, however, that the paradigm is not universal, so further research is required on a wide diversity of speci...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1e9902a3e82e93bac8d11a737460f069" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519504,"asset_id":53892182,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519504/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892182"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892182"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892182; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892182]").text(description); $(".js-view-count[data-work-id=53892182]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892182; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892182']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892182, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1e9902a3e82e93bac8d11a737460f069" } } $('.js-work-strip[data-work-id=53892182]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892182,"title":"Conservation physiology of marine fishes: state of the art and prospects for policy","translated_title":"","metadata":{"abstract":"The state of the art of research on the environmental physiology of marine fishes is reviewed from the perspective of how it can contribute to conservation of biodiversity and fishery resources. 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wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892179/Metabolic_and_molecular_stress_responses_of_the_gilthead_seabream_Sparus_aurata_during_long_term_exposure_to_increasing_temperatures">Metabolic and molecular stress responses of the gilthead seabream Sparus aurata during long-term exposure to increasing temperatures</a></div><div class="wp-workCard_item"><span>Epic3marine Biology 156 Pp 797 809</span><span>, Jan 2, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0f62963389df3146bf83d276cfece6e4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70520060,"asset_id":53892179,"asset_type":"Work","button_location":"profile"}" 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Prolonged Acclimation to Cold on the Extra- and Intracellular Acid-Base Status in the Land Snail [ITAL]Helix lucorum[/ITAL] (L.)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892176/Effects_of_Prolonged_Acclimation_to_Cold_on_the_Extra_and_Intracellular_Acid_Base_Status_in_the_Land_Snail_ITAL_Helix_lucorum_ITAL_L_">Effects of Prolonged Acclimation to Cold on the Extra- and Intracellular Acid-Base Status in the Land Snail [ITAL]Helix lucorum[/ITAL] (L.)</a></div><div class="wp-workCard_item"><span>Physiol Biochem Zool</span><span>, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The aim of this study was to examine the effect of prolonged acclimation to cold on the acid-base...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The aim of this study was to examine the effect of prolonged acclimation to cold on the acid-base status of extra-and in-tracellular fluids in the land snail Helix lucorum. For this pur-pose, acid-base parameters in the hemolymph and tissues were determined. In addition, the ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892176"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892176"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892176; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892176]").text(description); $(".js-view-count[data-work-id=53892176]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892176; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892176']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892176, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892176]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892176,"title":"Effects of Prolonged Acclimation to Cold on the Extra- and Intracellular Acid-Base Status in the Land Snail [ITAL]Helix lucorum[/ITAL] (L.)","translated_title":"","metadata":{"abstract":"The aim of this study was to examine the effect of prolonged acclimation to cold on the acid-base status of extra-and in-tracellular fluids in the land snail Helix lucorum. 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In addition, the ...","internal_url":"https://www.academia.edu/53892176/Effects_of_Prolonged_Acclimation_to_Cold_on_the_Extra_and_Intracellular_Acid_Base_Status_in_the_Land_Snail_ITAL_Helix_lucorum_ITAL_L_","translated_internal_url":"","created_at":"2021-09-29T01:51:51.240-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Effects_of_Prolonged_Acclimation_to_Cold_on_the_Extra_and_Intracellular_Acid_Base_Status_in_the_Land_Snail_ITAL_Helix_lucorum_ITAL_L_","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The aim of this study was to examine the effect of prolonged acclimation to cold on the acid-base status of extra-and in-tracellular fluids in the land snail Helix lucorum. For this pur-pose, acid-base parameters in the hemolymph and tissues were determined. In addition, the ...","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology"},{"id":395059,"name":"Land snail","url":"https://www.academia.edu/Documents/in/Land_snail"}],"urls":[{"id":11673949,"url":"http://journals.uchicago.edu/cgi-bin/resolve?id=doi:10.1086/320422"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892173"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892173/The_potential_role_of_CO2_in_initiation_and_maintenance_of_estivation_in_the_land_snail_Helix_lucorum"><img alt="Research paper thumbnail of The potential role of CO2 in initiation and maintenance of estivation in the land snail Helix lucorum" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892173/The_potential_role_of_CO2_in_initiation_and_maintenance_of_estivation_in_the_land_snail_Helix_lucorum">The potential role of CO2 in initiation and maintenance of estivation in the land snail Helix lucorum</a></div><div class="wp-workCard_item"><span>Epic3physiological and Biochemical Zoology 80 Pp 113 124</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Elevated CO(2) levels are hypothesized to play a role in the initiation and maintenance of estiva...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Elevated CO(2) levels are hypothesized to play a role in the initiation and maintenance of estivation in snails through disturbances of acid-base status. The aim of our study was to identify the ambient CO(2) threshold that induces disturbances in acid-base status in the air-breathing land snail Helix lucorum. Acid-base parameters were determined in the hemolymph of snails acclimated to 0.5%, 1%, 2%, 4%, and 8% CO(2) in air for 20 d. In addition, we evaluated the effects of long-term acclimation on metabolic rate and on levels of D-lactate dehydrogenase activity (D-LDH) and of D-lactate in snails after 20 d of exposure to increased CO(2) levels. Helix lucorum proved to be unable to compensate for a decrease in extracellular pH (pH(e)) when acclimated to levels higher than 1% CO(2) in air. The rate of oxygen consumption started to decrease when snails were acclimated to 0.5% CO(2) in air. However, there was no correlation between the drops in pH(e) and in metabolic rate. Long-term acclimation to elevated CO(2) levels induced an increase in the activity of D-LDH with a concomitant accumulation of D-lactate in tissues. This indicates that long-term acclimation to elevated ambient CO(2) levels could reduce the aerobic capacity of land snails and trigger expression of anaerobic pathways of ATP turnover. The threshold levels of ambient CO(2) that induce changes in acid-base status and elicit metabolic depression in adult land snails H. lucorum are higher than the future atmospheric levels that are expected to result from human use of fossil energy resources.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892173"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892173"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892173; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892173]").text(description); $(".js-view-count[data-work-id=53892173]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892173; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892173']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892173, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892173]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892173,"title":"The potential role of CO2 in initiation and maintenance of estivation in the land snail Helix lucorum","translated_title":"","metadata":{"abstract":"Elevated CO(2) levels are hypothesized to play a role in the initiation and maintenance of estivation in snails through disturbances of acid-base status. 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The aim of our study was to identify the ambient CO(2) threshold that induces disturbances in acid-base status in the air-breathing land snail Helix lucorum. Acid-base parameters were determined in the hemolymph of snails acclimated to 0.5%, 1%, 2%, 4%, and 8% CO(2) in air for 20 d. In addition, we evaluated the effects of long-term acclimation on metabolic rate and on levels of D-lactate dehydrogenase activity (D-LDH) and of D-lactate in snails after 20 d of exposure to increased CO(2) levels. Helix lucorum proved to be unable to compensate for a decrease in extracellular pH (pH(e)) when acclimated to levels higher than 1% CO(2) in air. The rate of oxygen consumption started to decrease when snails were acclimated to 0.5% CO(2) in air. However, there was no correlation between the drops in pH(e) and in metabolic rate. Long-term acclimation to elevated CO(2) levels induced an increase in the activity of D-LDH with a concomitant accumulation of D-lactate in tissues. This indicates that long-term acclimation to elevated ambient CO(2) levels could reduce the aerobic capacity of land snails and trigger expression of anaerobic pathways of ATP turnover. The threshold levels of ambient CO(2) that induce changes in acid-base status and elicit metabolic depression in adult land snails H. lucorum are higher than the future atmospheric levels that are expected to result from human use of fossil energy resources.","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology"},{"id":453528,"name":"Acclimatization","url":"https://www.academia.edu/Documents/in/Acclimatization"},{"id":544188,"name":"Hemolymph","url":"https://www.academia.edu/Documents/in/Hemolymph"},{"id":1193624,"name":"Oxygen Consumption","url":"https://www.academia.edu/Documents/in/Oxygen_Consumption"},{"id":2045642,"name":"Acid-Base Equilibrium","url":"https://www.academia.edu/Documents/in/Acid-Base_Equilibrium"}],"urls":[{"id":11673948,"url":"http://epic.awi.de/16077/"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892169"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892169/Field_studies_on_the_annual_activity_and_the_metabolic_responses_of_a_land_snail_population_living_in_high_altitude"><img alt="Research paper thumbnail of Field studies on the annual activity and the metabolic responses of a land snail population living in high altitude" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892169/Field_studies_on_the_annual_activity_and_the_metabolic_responses_of_a_land_snail_population_living_in_high_altitude">Field studies on the annual activity and the metabolic responses of a land snail population living in high altitude</a></div><div class="wp-workCard_item"><span>Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and activity cycle characteristics and b) the metabolic responses of the endemic land snail species Cattania trizona olympica living at 1100m altitude in Olympus mountain (Greece). Field observations on the annual activity cycle of C. trizona olympica revealed that snails&amp;#39; activity was restricted mainly between the end of May and September, when the higher temperatures were recorded, while first matings were recorded in July and the last ones in mid September indicating a restricted favorable time period for reproduction. The activities of enzymes of intermediate metabolism showed a periodic seasonal pattern of change which seems to be closely related to the pattern of annual changes of air temperature and most of them exhibited higher activities during the coldest and warmest periods of the year. Moreover the data indicate a distinct differentiation of fuel oxidation during arousal and reproductive periods with lipid oxidation, apart from carbohydrates, contributing significantly to ATP turnover during reproductive activity. The higher enzymatic activities, determined in the tissues of C. trizona olympica than the corresponding ones determined in the tissues of the land snail species living at low altitudes, might indicate higher sensitivity of the intermediate metabolism and ATP turnover in C. trizona olympica to changes in environmental factors. Although the latter seems to be in line with the MCA hypothesis, it needs further investigation on metabolic rates to support it.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892169"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892169"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892169; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892169]").text(description); $(".js-view-count[data-work-id=53892169]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892169; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892169']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892169, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892169]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892169,"title":"Field studies on the annual activity and the metabolic responses of a land snail population living in high altitude","translated_title":"","metadata":{"abstract":"In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and activity cycle characteristics and b) the metabolic responses of the endemic land snail species Cattania trizona olympica living at 1100m altitude in Olympus mountain (Greece). Field observations on the annual activity cycle of C. trizona olympica revealed that snails\u0026amp;#39; activity was restricted mainly between the end of May and September, when the higher temperatures were recorded, while first matings were recorded in July and the last ones in mid September indicating a restricted favorable time period for reproduction. The activities of enzymes of intermediate metabolism showed a periodic seasonal pattern of change which seems to be closely related to the pattern of annual changes of air temperature and most of them exhibited higher activities during the coldest and warmest periods of the year. Moreover the data indicate a distinct differentiation of fuel oxidation during arousal and reproductive periods with lipid oxidation, apart from carbohydrates, contributing significantly to ATP turnover during reproductive activity. The higher enzymatic activities, determined in the tissues of C. trizona olympica than the corresponding ones determined in the tissues of the land snail species living at low altitudes, might indicate higher sensitivity of the intermediate metabolism and ATP turnover in C. trizona olympica to changes in environmental factors. Although the latter seems to be in line with the MCA hypothesis, it needs further investigation on metabolic rates to support it.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Comparative Biochemistry and Physiology Part A: Molecular \u0026 Integrative Physiology"},"translated_abstract":"In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and activity cycle characteristics and b) the metabolic responses of the endemic land snail species Cattania trizona olympica living at 1100m altitude in Olympus mountain (Greece). Field observations on the annual activity cycle of C. trizona olympica revealed that snails\u0026amp;#39; activity was restricted mainly between the end of May and September, when the higher temperatures were recorded, while first matings were recorded in July and the last ones in mid September indicating a restricted favorable time period for reproduction. The activities of enzymes of intermediate metabolism showed a periodic seasonal pattern of change which seems to be closely related to the pattern of annual changes of air temperature and most of them exhibited higher activities during the coldest and warmest periods of the year. Moreover the data indicate a distinct differentiation of fuel oxidation during arousal and reproductive periods with lipid oxidation, apart from carbohydrates, contributing significantly to ATP turnover during reproductive activity. The higher enzymatic activities, determined in the tissues of C. trizona olympica than the corresponding ones determined in the tissues of the land snail species living at low altitudes, might indicate higher sensitivity of the intermediate metabolism and ATP turnover in C. trizona olympica to changes in environmental factors. Although the latter seems to be in line with the MCA hypothesis, it needs further investigation on metabolic rates to support it.","internal_url":"https://www.academia.edu/53892169/Field_studies_on_the_annual_activity_and_the_metabolic_responses_of_a_land_snail_population_living_in_high_altitude","translated_internal_url":"","created_at":"2021-09-29T01:51:50.744-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Field_studies_on_the_annual_activity_and_the_metabolic_responses_of_a_land_snail_population_living_in_high_altitude","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and activity cycle characteristics and b) the metabolic responses of the endemic land snail species Cattania trizona olympica living at 1100m altitude in Olympus mountain (Greece). Field observations on the annual activity cycle of C. trizona olympica revealed that snails\u0026amp;#39; activity was restricted mainly between the end of May and September, when the higher temperatures were recorded, while first matings were recorded in July and the last ones in mid September indicating a restricted favorable time period for reproduction. The activities of enzymes of intermediate metabolism showed a periodic seasonal pattern of change which seems to be closely related to the pattern of annual changes of air temperature and most of them exhibited higher activities during the coldest and warmest periods of the year. Moreover the data indicate a distinct differentiation of fuel oxidation during arousal and reproductive periods with lipid oxidation, apart from carbohydrates, contributing significantly to ATP turnover during reproductive activity. The higher enzymatic activities, determined in the tissues of C. trizona olympica than the corresponding ones determined in the tissues of the land snail species living at low altitudes, might indicate higher sensitivity of the intermediate metabolism and ATP turnover in C. trizona olympica to changes in environmental factors. Although the latter seems to be in line with the MCA hypothesis, it needs further investigation on metabolic rates to support it.","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics"},{"id":48044,"name":"Greece","url":"https://www.academia.edu/Documents/in/Greece"},{"id":186440,"name":"Snails","url":"https://www.academia.edu/Documents/in/Snails"},{"id":577239,"name":"Altitude","url":"https://www.academia.edu/Documents/in/Altitude"},{"id":649451,"name":"Seasons","url":"https://www.academia.edu/Documents/in/Seasons"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892166"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892166/The_possible_role_of_glycolytic_enzyme_binding_in_the_control_of_glycolysis_in_Patella_caerulea_foot_muscle_during_stimulation"><img alt="Research paper thumbnail of The possible role of glycolytic enzyme binding in the control of glycolysis in Patella caerulea foot muscle during stimulation" class="work-thumbnail" src="https://attachments.academia-assets.com/70519495/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892166/The_possible_role_of_glycolytic_enzyme_binding_in_the_control_of_glycolysis_in_Patella_caerulea_foot_muscle_during_stimulation">The possible role of glycolytic enzyme binding in the control of glycolysis in Patella caerulea foot muscle during stimulation</a></div><div class="wp-workCard_item"><span>Comparative Biochemistry and Physiology Part B: Comparative Biochemistry</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The extent of glycolytic enzyme activity associated with the particulate fraction of homogenates ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The extent of glycolytic enzyme activity associated with the particulate fraction of homogenates was measured in P. caerulea foot muscle at rest and after electrical stimulation. 2. In association with the increased glycolytic rate on stimulation, there was a significant increase in the binding of certain enzymes such as phosphorylase, phosphoglucomutase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase. 3. It is concluded that the variable enzyme binding to the particulate fraction could constitute an additional mechanism for metabolic control in P. caerulea foot muscle.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6c170981d9b04a544c3840287d253072" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519495,"asset_id":53892166,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519495/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892166"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892166"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892166; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892166]").text(description); $(".js-view-count[data-work-id=53892166]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892166; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892166']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892166, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6c170981d9b04a544c3840287d253072" } } $('.js-work-strip[data-work-id=53892166]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892166,"title":"The possible role of glycolytic enzyme binding in the control of glycolysis in Patella caerulea foot muscle during stimulation","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"The extent of glycolytic enzyme activity associated with the particulate fraction of homogenates was measured in P. caerulea foot muscle at rest and after electrical stimulation. 2. In association with the increased glycolytic rate on stimulation, there was a significant increase in the binding of certain enzymes such as phosphorylase, phosphoglucomutase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase. 3. 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In association with the increased glycolytic rate on stimulation, there was a significant increase in the binding of certain enzymes such as phosphorylase, phosphoglucomutase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase. 3. It is concluded that the variable enzyme binding to the particulate fraction could constitute an additional mechanism for metabolic control in P. caerulea foot muscle.","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[{"id":70519495,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/70519495/thumbnails/1.jpg","file_name":"0305-0491_2889_2990076-x20210929-5742-5d3hct.pdf","download_url":"https://www.academia.edu/attachments/70519495/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_possible_role_of_glycolytic_enzyme_b.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/70519495/0305-0491_2889_2990076-x20210929-5742-5d3hct-libre.pdf?1632906060=\u0026response-content-disposition=attachment%3B+filename%3DThe_possible_role_of_glycolytic_enzyme_b.pdf\u0026Expires=1733952068\u0026Signature=B9KPlCTjTaO3qdMKy35tBbKaXSVY-R5RoXUQ0n0FvWmzFbXsD5tBQwhWQZf5fD8u0RIg7~73xtvgOHxfWeILcyo~zHwA1VEP-Atk2VFF9xDlm0UMKMbPlcxcgsMBiJdDVvZW-5yIcns7mBkJoSxdBSue1UuEhbVGRj4slttse00xssVKyy2KBhi3DSaKcvHv3twy~pk~ZvsGMjjSPcHQ8asUy1KVEQ5bX3aDEXFNNfRTP41TLHgbFy58cX3se18JxF~HgUftHQT0TS~KAkgCtdEkL9muLfwp9uPaK2PvNulMxWkZuUl2TayuGqJozkHUe80TQUhu8qZPtFYwhn9eeg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":216773,"name":"Comparative Biochemistry and Physiology","url":"https://www.academia.edu/Documents/in/Comparative_Biochemistry_and_Physiology"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892164"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892164/Regulation_of_pyruvate_kinase_PK_from_the_ventricle_of_the_land_snail_Helix_lucorum_L_during_early_and_prolonged_estivation_and_hibernation"><img alt="Research paper thumbnail of Regulation of pyruvate kinase (PK) from the ventricle of the land snail Helix lucorum L. during early and prolonged estivation and hibernation" class="work-thumbnail" src="https://attachments.academia-assets.com/70519632/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892164/Regulation_of_pyruvate_kinase_PK_from_the_ventricle_of_the_land_snail_Helix_lucorum_L_during_early_and_prolonged_estivation_and_hibernation">Regulation of pyruvate kinase (PK) from the ventricle of the land snail Helix lucorum L. during early and prolonged estivation and hibernation</a></div><div class="wp-workCard_item"><span>Comparative Biochemistry and Physiology Part B: Comparative Biochemistry</span><span>, 1994</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The kinetic properties of pyruvate kinase (PK) from the ventricles of early and prolonged estivat...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The kinetic properties of pyruvate kinase (PK) from the ventricles of early and prolonged estivated and hibernated land snails, Helix lucorum, were studied. The kinetic properties of PK from the ventricles of snails accfimated to cold for 4 days and 2 months were also determined. Short estivation (4 days) converted the PK to a less active form, possibly via enzyme phosphorylation. After 2 months of estivation, PK exhibited kinetic properties similar to control enzyme, except that the Vmx increased about 2-fold, indicating metabolic reorganization. During early and prolonged hibernation, the kinetic properties of PK did not change significantly. The results indicate that low temperature and low pH may be the main modulators of enzyme activity in the ventricle of H. iucorum during hibernation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0c92bc8b14f2d61e4bcc4046f2f6888a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519632,"asset_id":53892164,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519632/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892164"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892164"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892164; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892164]").text(description); $(".js-view-count[data-work-id=53892164]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892164; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892164']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892164, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0c92bc8b14f2d61e4bcc4046f2f6888a" } } $('.js-work-strip[data-work-id=53892164]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892164,"title":"Regulation of pyruvate kinase (PK) from the ventricle of the land snail Helix lucorum L. during early and prolonged estivation and hibernation","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"The kinetic properties of pyruvate kinase (PK) from the ventricles of early and prolonged estivated and hibernated land snails, Helix lucorum, were studied. The kinetic properties of PK from the ventricles of snails accfimated to cold for 4 days and 2 months were also determined. Short estivation (4 days) converted the PK to a less active form, possibly via enzyme phosphorylation. After 2 months of estivation, PK exhibited kinetic properties similar to control enzyme, except that the Vmx increased about 2-fold, indicating metabolic reorganization. During early and prolonged hibernation, the kinetic properties of PK did not change significantly. The results indicate that low temperature and low pH may be the main modulators of enzyme activity in the ventricle of H. iucorum during hibernation.","publication_date":{"day":null,"month":null,"year":1994,"errors":{}},"publication_name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","grobid_abstract_attachment_id":70519632},"translated_abstract":null,"internal_url":"https://www.academia.edu/53892164/Regulation_of_pyruvate_kinase_PK_from_the_ventricle_of_the_land_snail_Helix_lucorum_L_during_early_and_prolonged_estivation_and_hibernation","translated_internal_url":"","created_at":"2021-09-29T01:51:50.537-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":70519632,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/70519632/thumbnails/1.jpg","file_name":"0305-0491_2894_2990189-920210929-12269-1s9w8qd.pdf","download_url":"https://www.academia.edu/attachments/70519632/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Regulation_of_pyruvate_kinase_PK_from_th.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/70519632/0305-0491_2894_2990189-920210929-12269-1s9w8qd-libre.pdf?1632906053=\u0026response-content-disposition=attachment%3B+filename%3DRegulation_of_pyruvate_kinase_PK_from_th.pdf\u0026Expires=1733952068\u0026Signature=E6FYQgpcW4oCKjKgU73zQ4Uu0Cp39ucrx0aja3oHpqnElnsYOr7pmcdNXCMCQjnDPHydsLq9nKfssVpE4WHk0K4dEiBSbJD-xBHX7G2sbwLfk4Zi9QRLaxRYVJkE0kJuVGJ4VlzCrzcAvNf1NZ~9Wbdld8ZveBY5FD1Fozjcg1ZFD0uvHmnJiqI8tOB00ouk0ycIOwlmunAJ27F~lGJjqHhOqd3DphKa0ajTuj4wNpzKVGQEtB-mlcpsdqwziZWes~ad7IBzlSh0hhcVfFQxfG64SM7a-yvbdHc-3puAU03smrB-uSR6uhIN8FjwT6zg2moWYZ4CjnZFk1PLnwD3pw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Regulation_of_pyruvate_kinase_PK_from_the_ventricle_of_the_land_snail_Helix_lucorum_L_during_early_and_prolonged_estivation_and_hibernation","translated_slug":"","page_count":7,"language":"en","content_type":"Work","summary":"The kinetic properties of pyruvate kinase (PK) from the ventricles of early and prolonged estivated and hibernated land snails, Helix lucorum, were studied. The kinetic properties of PK from the ventricles of snails accfimated to cold for 4 days and 2 months were also determined. Short estivation (4 days) converted the PK to a less active form, possibly via enzyme phosphorylation. After 2 months of estivation, PK exhibited kinetic properties similar to control enzyme, except that the Vmx increased about 2-fold, indicating metabolic reorganization. During early and prolonged hibernation, the kinetic properties of PK did not change significantly. The results indicate that low temperature and low pH may be the main modulators of enzyme activity in the ventricle of H. iucorum during hibernation.","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[{"id":70519632,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/70519632/thumbnails/1.jpg","file_name":"0305-0491_2894_2990189-920210929-12269-1s9w8qd.pdf","download_url":"https://www.academia.edu/attachments/70519632/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Regulation_of_pyruvate_kinase_PK_from_th.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/70519632/0305-0491_2894_2990189-920210929-12269-1s9w8qd-libre.pdf?1632906053=\u0026response-content-disposition=attachment%3B+filename%3DRegulation_of_pyruvate_kinase_PK_from_th.pdf\u0026Expires=1733952068\u0026Signature=E6FYQgpcW4oCKjKgU73zQ4Uu0Cp39ucrx0aja3oHpqnElnsYOr7pmcdNXCMCQjnDPHydsLq9nKfssVpE4WHk0K4dEiBSbJD-xBHX7G2sbwLfk4Zi9QRLaxRYVJkE0kJuVGJ4VlzCrzcAvNf1NZ~9Wbdld8ZveBY5FD1Fozjcg1ZFD0uvHmnJiqI8tOB00ouk0ycIOwlmunAJ27F~lGJjqHhOqd3DphKa0ajTuj4wNpzKVGQEtB-mlcpsdqwziZWes~ad7IBzlSh0hhcVfFQxfG64SM7a-yvbdHc-3puAU03smrB-uSR6uhIN8FjwT6zg2moWYZ4CjnZFk1PLnwD3pw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics"},{"id":11035,"name":"Regulation","url":"https://www.academia.edu/Documents/in/Regulation"},{"id":45304,"name":"Hibernation","url":"https://www.academia.edu/Documents/in/Hibernation"},{"id":71434,"name":"Pyruvate Kinase","url":"https://www.academia.edu/Documents/in/Pyruvate_Kinase"},{"id":172083,"name":"Phosphorylation","url":"https://www.academia.edu/Documents/in/Phosphorylation"},{"id":172897,"name":"Glycolysis","url":"https://www.academia.edu/Documents/in/Glycolysis"},{"id":395059,"name":"Land snail","url":"https://www.academia.edu/Documents/in/Land_snail"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892161"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892161/Studies_on_the_anaerobic_energy_metabolism_in_the_foot_muscle_of_marine_gastropod_Patella_caerulea_L_"><img alt="Research paper thumbnail of Studies on the anaerobic energy metabolism in the foot muscle of marine gastropod Patella caerulea (L.)" class="work-thumbnail" src="https://attachments.academia-assets.com/70519633/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892161/Studies_on_the_anaerobic_energy_metabolism_in_the_foot_muscle_of_marine_gastropod_Patella_caerulea_L_">Studies on the anaerobic energy metabolism in the foot muscle of marine gastropod Patella caerulea (L.)</a></div><div class="wp-workCard_item"><span>Comparative Biochemistry and Physiology Part B: Comparative Biochemistry</span><span>, 1990</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The foot muscle of P. caerulea has a complete sequence of glycolytic enzymes. The low activity of...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The foot muscle of P. caerulea has a complete sequence of glycolytic enzymes. The low activity of hexokinase, in comparison with the activities of glycogen phosphorylase and phosphofructokinase, indicate that glycogen is the main fuel oxidized. 2. The reduction of aspartate content in combination with the accumulation of alanine and the presence of considerable activities of glutamate-oxaloacetate transaminase and glutamate~pyruvate transaminase indicates a coupled metabolism of glycogen and aspartate during exposure to air. 3. From the changes in the concentration of the metabolites during exposure to air it appears that up to the second hour of anaerobiosis alanine, lactate and glutamate are the end-products which accumulate in the foot muscle of P. caerulea, whereas from the second to the fourth hour only succinate and alanine accumulate. 4. The low activities of the Krebs cycle enzymes as well as the absence of ~t-ketoglutarate dehydrogenase activity suggest that the Krebs cycle is not in operation. 5. The absence of opine dehydrogenases shows that the end products octopine, alanopine and strombine are not accumulated in the foot muscle under anaerobiosis. 6. The high activity of malate dehydrogenase in the direction of malate formation in combination with the low activity of ~-lactate dehydrogenase and the absence of opine dehydrogenases suggests that the former dehydrogenase is coupled 1 : 1 to glyceraldehyde-3-phosphate dehydrogenase.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="203a24af0561af2c72c4900c9a519766" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519633,"asset_id":53892161,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519633/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892161"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892161"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892161; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892161]").text(description); $(".js-view-count[data-work-id=53892161]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892161; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892161']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892161, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "203a24af0561af2c72c4900c9a519766" } } $('.js-work-strip[data-work-id=53892161]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892161,"title":"Studies on the anaerobic energy metabolism in the foot muscle of marine gastropod Patella caerulea (L.)","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"The foot muscle of P. caerulea has a complete sequence of glycolytic enzymes. 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Such temperature extremes can stimulate aerobic metabolism toward its maximum, a decline in aerobic substrate oxidation, and a parallel increase of anaerobic metabolism, combined with ROS generation and oxidative stress. Under these stressful conditions, marine organisms recruit several defensive strategies for their maintenance and survival. However, thermal tolerance of ectothermic organisms may be increased after a brief exposure to sub-lethal temperatures, a process known as &quot;hardening&quot;. In our study, we examined the ability of M. galloprovincialis to increase its thermal tolerance under the effect of elevated temperatures (24, 26 and 28聽掳C) through the &quot;hardening&quot; process. Our results demonstrate that this process can increase the heat tolerance and antioxidant defense of heat hardened mussels through more efficient ETS activity when expos...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c59e23830963e45dc116167f23bd18c9" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":84945231,"asset_id":77626234,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/84945231/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2Nyw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77626234"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77626234"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77626234; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77626234]").text(description); $(".js-view-count[data-work-id=77626234]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77626234; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77626234']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77626234, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c59e23830963e45dc116167f23bd18c9" } } $('.js-work-strip[data-work-id=77626234]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77626234,"title":"Heat hardening enhances mitochondrial potential for respiration and oxidative defence capacity in the mantle of thermally stressed Mytilus galloprovincialis","translated_title":"","metadata":{"abstract":"Ectotherms are exposed to a range of environmental temperatures and may face extremes beyond their upper thermal limits. Such temperature extremes can stimulate aerobic metabolism toward its maximum, a decline in aerobic substrate oxidation, and a parallel increase of anaerobic metabolism, combined with ROS generation and oxidative stress. Under these stressful conditions, marine organisms recruit several defensive strategies for their maintenance and survival. However, thermal tolerance of ectothermic organisms may be increased after a brief exposure to sub-lethal temperatures, a process known as \u0026quot;hardening\u0026quot;. In our study, we examined the ability of M. galloprovincialis to increase its thermal tolerance under the effect of elevated temperatures (24, 26 and 28聽掳C) through the \u0026quot;hardening\u0026quot; process. Our results demonstrate that this process can increase the heat tolerance and antioxidant defense of heat hardened mussels through more efficient ETS activity when expos...","publisher":"Springer Science and Business Media LLC","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"Scientific Reports"},"translated_abstract":"Ectotherms are exposed to a range of environmental temperatures and may face extremes beyond their upper thermal limits. Such temperature extremes can stimulate aerobic metabolism toward its maximum, a decline in aerobic substrate oxidation, and a parallel increase of anaerobic metabolism, combined with ROS generation and oxidative stress. Under these stressful conditions, marine organisms recruit several defensive strategies for their maintenance and survival. However, thermal tolerance of ectothermic organisms may be increased after a brief exposure to sub-lethal temperatures, a process known as \u0026quot;hardening\u0026quot;. In our study, we examined the ability of M. galloprovincialis to increase its thermal tolerance under the effect of elevated temperatures (24, 26 and 28聽掳C) through the \u0026quot;hardening\u0026quot; process. Our results demonstrate that this process can increase the heat tolerance and antioxidant defense of heat hardened mussels through more efficient ETS activity when expos...","internal_url":"https://www.academia.edu/77626234/Heat_hardening_enhances_mitochondrial_potential_for_respiration_and_oxidative_defence_capacity_in_the_mantle_of_thermally_stressed_Mytilus_galloprovincialis","translated_internal_url":"","created_at":"2022-04-25T22:31:04.921-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":84945231,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/84945231/thumbnails/1.jpg","file_name":"s41598-021-96617-9.pdf","download_url":"https://www.academia.edu/attachments/84945231/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2Nyw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Heat_hardening_enhances_mitochondrial_po.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/84945231/s41598-021-96617-9-libre.pdf?1650953288=\u0026response-content-disposition=attachment%3B+filename%3DHeat_hardening_enhances_mitochondrial_po.pdf\u0026Expires=1733952067\u0026Signature=ELVpH62Tbe5Bst~7Z6dhaaVnz3NFzy6Qc4~dcUn2yjdLLSJz18eZ6dJRiksV2kuTPu8ZNK~TdK13ZNQpVee-a5p0h~YImPYaZ6R06g8qvXBgEitaUu7vAlb0rrVXfWBBOL8bXLXUFFE3fBIhgLz81V8yw7LZ760H6XFHqtf2e8j2ap-suIQo3gjAoOCjsoiX4dRofkgtaN9cc9-zAS7jx6VKoDcBUG7zt7Yc2Jb3LgIK5tbkbfMNcX2ZDFmZQRiqyY4~IiJfn30u8WaON5sgJB69v-~dKQSqBCZ17lgUBUmm1InA75A0kSEQUyJtIJJ6cC4FmPSI71jqlM0Kiod~JA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Heat_hardening_enhances_mitochondrial_potential_for_respiration_and_oxidative_defence_capacity_in_the_mantle_of_thermally_stressed_Mytilus_galloprovincialis","translated_slug":"","page_count":18,"language":"en","content_type":"Work","summary":"Ectotherms are exposed to a range of environmental temperatures and may face extremes beyond their upper thermal limits. Such temperature extremes can stimulate aerobic metabolism toward its maximum, a decline in aerobic substrate oxidation, and a parallel increase of anaerobic metabolism, combined with ROS generation and oxidative stress. Under these stressful conditions, marine organisms recruit several defensive strategies for their maintenance and survival. However, thermal tolerance of ectothermic organisms may be increased after a brief exposure to sub-lethal temperatures, a process known as \u0026quot;hardening\u0026quot;. In our study, we examined the ability of M. galloprovincialis to increase its thermal tolerance under the effect of elevated temperatures (24, 26 and 28聽掳C) through the \u0026quot;hardening\u0026quot; process. 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Could systematic monitoring and early warning prevent negative impacts A review focused on Vistonikos gulf, North Aegean Sea" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892207/The_effect_of_seawater_physical_parameters_in_bivalve_farming_Could_systematic_monitoring_and_early_warning_prevent_negative_impacts_A_review_focused_on_Vistonikos_gulf_North_Aegean_Sea">The effect of seawater physical parameters in bivalve farming. Could systematic monitoring and early warning prevent negative impacts A review focused on Vistonikos gulf, North Aegean Sea</a></div><div class="wp-workCard_item"><span>International Journal of Agricultural Resources, Governance and Ecology</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892207"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892207"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892207; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892207]").text(description); $(".js-view-count[data-work-id=53892207]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892207; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892207']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892207, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892207]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892207,"title":"The effect of seawater physical parameters in bivalve farming. Could systematic monitoring and early warning prevent negative impacts A review focused on Vistonikos gulf, North Aegean Sea","translated_title":"","metadata":{"publisher":"Inderscience Publishers","publication_name":"International Journal of Agricultural Resources, Governance and Ecology"},"translated_abstract":null,"internal_url":"https://www.academia.edu/53892207/The_effect_of_seawater_physical_parameters_in_bivalve_farming_Could_systematic_monitoring_and_early_warning_prevent_negative_impacts_A_review_focused_on_Vistonikos_gulf_North_Aegean_Sea","translated_internal_url":"","created_at":"2021-09-29T01:51:53.000-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_effect_of_seawater_physical_parameters_in_bivalve_farming_Could_systematic_monitoring_and_early_warning_prevent_negative_impacts_A_review_focused_on_Vistonikos_gulf_North_Aegean_Sea","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":null,"owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[],"research_interests":[{"id":27659,"name":"Applied Economics","url":"https://www.academia.edu/Documents/in/Applied_Economics"}],"urls":[{"id":11673961,"url":"http://www.inderscienceonline.com/doi/full/10.1504/IJARGE.2021.10041385"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892204"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892204/Gut_Symbiotic_Microbial_Communities_in_the_IUCN_Critically_Endangered_Pinna_nobilis_Suffering_from_Mass_Mortalities_Revealed_by_16S_rRNA_Amplicon_NGS"><img alt="Research paper thumbnail of Gut Symbiotic Microbial Communities in the IUCN Critically Endangered Pinna nobilis Suffering from Mass Mortalities, Revealed by 16S rRNA Amplicon NGS" class="work-thumbnail" src="https://attachments.academia-assets.com/70519394/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892204/Gut_Symbiotic_Microbial_Communities_in_the_IUCN_Critically_Endangered_Pinna_nobilis_Suffering_from_Mass_Mortalities_Revealed_by_16S_rRNA_Amplicon_NGS">Gut Symbiotic Microbial Communities in the IUCN Critically Endangered Pinna nobilis Suffering from Mass Mortalities, Revealed by 16S rRNA Amplicon NGS</a></div><div class="wp-workCard_item"><span>Pathogens</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mass mortality events due to disease outbreaks have recently affected almost every healthy popula...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mass mortality events due to disease outbreaks have recently affected almost every healthy population of fan mussel, Pinna nobilis in Mediterranean Sea. The devastating mortality of the species has turned the interest of the research towards the causes of these events. After the haplosporidan infestation and the infection by Mycobacterium sp., new emerging pathogens have arisen based on the latest research. In the present study, a metagenomic approach of 16S rRNA next generation sequencing (NGS) was applied in order to assess the bacterial diversity within the digestive gland of diseased individuals as well as to carry out geographical correlations among the biodiversity of microbiome in the endangered species Pinna nobilis. The specimens originated from the mortalities occurred in 2019 in the region of Greece. Together with other bacterial genera, the results confirmed the presence of Vibrio spp., assuming synergistic effects in the mortality events of the species. Alongside with t...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="16a4e594b4e0812e65e57541575d0d12" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519394,"asset_id":53892204,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519394/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892204"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892204"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892204; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892204]").text(description); $(".js-view-count[data-work-id=53892204]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892204; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892204']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892204, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "16a4e594b4e0812e65e57541575d0d12" } } $('.js-work-strip[data-work-id=53892204]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892204,"title":"Gut Symbiotic Microbial Communities in the IUCN Critically Endangered Pinna nobilis Suffering from Mass Mortalities, Revealed by 16S rRNA Amplicon NGS","translated_title":"","metadata":{"abstract":"Mass mortality events due to disease outbreaks have recently affected almost every healthy population of fan mussel, Pinna nobilis in Mediterranean Sea. 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href="https://www.academia.edu/53892191/Estimating_cage_farming_capacity_of_data_poor_mariculture_sectors_in_the_Eastern_Mediterranean"><img alt="Research paper thumbnail of Estimating cage farming capacity of data-poor mariculture sectors in the Eastern Mediterranean" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892191/Estimating_cage_farming_capacity_of_data_poor_mariculture_sectors_in_the_Eastern_Mediterranean">Estimating cage farming capacity of data-poor mariculture sectors in the Eastern Mediterranean</a></div><div class="wp-workCard_item"><span>Journal of Applied Aquaculture</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892191"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892191"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892191; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892191]").text(description); $(".js-view-count[data-work-id=53892191]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget 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status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892189/First_insights_towards_the_population_genetic_structure_and_the_phylogeographic_status_of_the_horse_mussel_Modiolus_barbatus_from_the_eastern_Mediterranean">First insights towards the population genetic structure and the phylogeographic status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean</a></div><div class="wp-workCard_item"><span>Journal of the Marine Biological Association of the United Kingdom</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The horse mussel Modiolus barbatus is a marine benthic bivalve, distributed mainly in the Mediter...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The horse mussel Modiolus barbatus is a marine benthic bivalve, distributed mainly in the Mediterranean basin, that constitutes a fishery product of high economic importance and a promising candidate for aquaculture. The current study provides the first insights regarding the genetic profile of M. barbatus populations from the eastern Mediterranean, by analysis of a partial segment of the mitochondrial COI gene in individuals collected from five sampling localities within the Aegean Sea. To the best of our knowledge, the derived haplotypes represent the first DNA barcodes of M. barbatus from the entire Mediterranean region. Maximum likelihood phylogenetic analysis revealed that M. barbatus from the North Sea and M. barbatus from the eastern Mediterranean may not belong to the same species and as a consequence, there might be three species of the genus Modiolus in Europe. On the other hand, eastern Mediterranean M. barbatus haplotypes were found to be more closely related to the Asia...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892189"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892189"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892189; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892189]").text(description); $(".js-view-count[data-work-id=53892189]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892189; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892189']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892189, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892189]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892189,"title":"First insights towards the population genetic structure and the phylogeographic status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean","translated_title":"","metadata":{"abstract":"The horse mussel Modiolus barbatus is a marine benthic bivalve, distributed mainly in the Mediterranean basin, that constitutes a fishery product of high economic importance and a promising candidate for aquaculture. The current study provides the first insights regarding the genetic profile of M. barbatus populations from the eastern Mediterranean, by analysis of a partial segment of the mitochondrial COI gene in individuals collected from five sampling localities within the Aegean Sea. To the best of our knowledge, the derived haplotypes represent the first DNA barcodes of M. barbatus from the entire Mediterranean region. Maximum likelihood phylogenetic analysis revealed that M. barbatus from the North Sea and M. barbatus from the eastern Mediterranean may not belong to the same species and as a consequence, there might be three species of the genus Modiolus in Europe. 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On the other hand, eastern Mediterranean M. barbatus haplotypes were found to be more closely related to the Asia...","internal_url":"https://www.academia.edu/53892189/First_insights_towards_the_population_genetic_structure_and_the_phylogeographic_status_of_the_horse_mussel_Modiolus_barbatus_from_the_eastern_Mediterranean","translated_internal_url":"","created_at":"2021-09-29T01:51:51.955-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"First_insights_towards_the_population_genetic_structure_and_the_phylogeographic_status_of_the_horse_mussel_Modiolus_barbatus_from_the_eastern_Mediterranean","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The horse mussel Modiolus barbatus is a marine benthic bivalve, distributed mainly in the Mediterranean basin, that constitutes a fishery product of high economic importance and a promising candidate for aquaculture. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892184"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892184/Seasonal_Changes_in_Metabolism_and_Cellular_Stress_Phenomena_in_the_Gilthead_Sea_Bream_Sparus_aurata_"><img alt="Research paper thumbnail of Seasonal Changes in Metabolism and Cellular Stress Phenomena in the Gilthead Sea Bream (Sparus aurata)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892184/Seasonal_Changes_in_Metabolism_and_Cellular_Stress_Phenomena_in_the_Gilthead_Sea_Bream_Sparus_aurata_">Seasonal Changes in Metabolism and Cellular Stress Phenomena in the Gilthead Sea Bream (Sparus aurata)</a></div><div class="wp-workCard_item"><span>Physiological and biochemical zoology : PBZ</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Seasonal temperature changes may take organisms to the upper and lower limit of their thermal ran...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Seasonal temperature changes may take organisms to the upper and lower limit of their thermal range, with respective variations in their biochemical and metabolic profile. To elucidate these traits, we investigated metabolic and antioxidant patterns in tissues of sea bream Sparus aurata during seasonal acclimatization for 1 yr in the field. Metabolic patterns were assessed by determining lactate dehydrogenase, citrate synthase, and 尾-hydroxyacyl CoA dehydrogenase activities, their kinetic properties and plasma levels of glucose, lactate, and triglycerides and tissue succinate levels. Oxidative stress was assessed by determining antioxidant enzymes superoxide dismutase, catalase, and glutathione reductase activities and levels of thiobarbituric acid reactive substances. Xanthine oxidase (XO) activity was determined as another source of reactive oxygen species (ROS) production. 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A major constraint to application of physiological knowledge for conservation of marine fishes is the limited knowledge base; international collaboration is needed to study the environmental physiology of a wider range of species. Multifactorial field and laboratory studies on biomarkers hold promise to relate ecophysiology directly to habitat quality and population status. The &#39;Fry paradigm&#39; could have broad applications for conservation physiology research if it provides a universal mechanism to link physiological function with ecological performance and population dynamics of fishes, through effects of abiotic conditions on aerobic metabolic scope. The available data indicate, however, that the paradigm is not universal, so further research is required on a wide diversity of speci...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1e9902a3e82e93bac8d11a737460f069" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519504,"asset_id":53892182,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519504/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892182"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892182"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892182; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892182]").text(description); $(".js-view-count[data-work-id=53892182]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892182; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892182']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892182, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1e9902a3e82e93bac8d11a737460f069" } } $('.js-work-strip[data-work-id=53892182]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892182,"title":"Conservation physiology of marine fishes: state of the art and prospects for policy","translated_title":"","metadata":{"abstract":"The state of the art of research on the environmental physiology of marine fishes is reviewed from the perspective of how it can contribute to conservation of biodiversity and fishery resources. 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A major constraint to application of physiological knowledge for conservation of marine fishes is the limited knowledge base; international collaboration is needed to study the environmental physiology of a wider range of species. Multifactorial field and laboratory studies on biomarkers hold promise to relate ecophysiology directly to habitat quality and population status. The \u0026#39;Fry paradigm\u0026#39; could have broad applications for conservation physiology research if it provides a universal mechanism to link physiological function with ecological performance and population dynamics of fishes, through effects of abiotic conditions on aerobic metabolic scope. 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Prolonged Acclimation to Cold on the Extra- and Intracellular Acid-Base Status in the Land Snail [ITAL]Helix lucorum[/ITAL] (L.)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892176/Effects_of_Prolonged_Acclimation_to_Cold_on_the_Extra_and_Intracellular_Acid_Base_Status_in_the_Land_Snail_ITAL_Helix_lucorum_ITAL_L_">Effects of Prolonged Acclimation to Cold on the Extra- and Intracellular Acid-Base Status in the Land Snail [ITAL]Helix lucorum[/ITAL] (L.)</a></div><div class="wp-workCard_item"><span>Physiol Biochem Zool</span><span>, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The aim of this study was to examine the effect of prolonged acclimation to cold on the acid-base...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The aim of this study was to examine the effect of prolonged acclimation to cold on the acid-base status of extra-and in-tracellular fluids in the land snail Helix lucorum. For this pur-pose, acid-base parameters in the hemolymph and tissues were determined. In addition, the ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892176"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892176"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892176; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892176]").text(description); $(".js-view-count[data-work-id=53892176]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892176; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892176']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892176, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892176]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892176,"title":"Effects of Prolonged Acclimation to Cold on the Extra- and Intracellular Acid-Base Status in the Land Snail [ITAL]Helix lucorum[/ITAL] (L.)","translated_title":"","metadata":{"abstract":"The aim of this study was to examine the effect of prolonged acclimation to cold on the acid-base status of extra-and in-tracellular fluids in the land snail Helix lucorum. 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In addition, the ...","internal_url":"https://www.academia.edu/53892176/Effects_of_Prolonged_Acclimation_to_Cold_on_the_Extra_and_Intracellular_Acid_Base_Status_in_the_Land_Snail_ITAL_Helix_lucorum_ITAL_L_","translated_internal_url":"","created_at":"2021-09-29T01:51:51.240-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Effects_of_Prolonged_Acclimation_to_Cold_on_the_Extra_and_Intracellular_Acid_Base_Status_in_the_Land_Snail_ITAL_Helix_lucorum_ITAL_L_","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The aim of this study was to examine the effect of prolonged acclimation to cold on the acid-base status of extra-and in-tracellular fluids in the land snail Helix lucorum. For this pur-pose, acid-base parameters in the hemolymph and tissues were determined. In addition, the ...","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology"},{"id":395059,"name":"Land snail","url":"https://www.academia.edu/Documents/in/Land_snail"}],"urls":[{"id":11673949,"url":"http://journals.uchicago.edu/cgi-bin/resolve?id=doi:10.1086/320422"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892173"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892173/The_potential_role_of_CO2_in_initiation_and_maintenance_of_estivation_in_the_land_snail_Helix_lucorum"><img alt="Research paper thumbnail of The potential role of CO2 in initiation and maintenance of estivation in the land snail Helix lucorum" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892173/The_potential_role_of_CO2_in_initiation_and_maintenance_of_estivation_in_the_land_snail_Helix_lucorum">The potential role of CO2 in initiation and maintenance of estivation in the land snail Helix lucorum</a></div><div class="wp-workCard_item"><span>Epic3physiological and Biochemical Zoology 80 Pp 113 124</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Elevated CO(2) levels are hypothesized to play a role in the initiation and maintenance of estiva...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Elevated CO(2) levels are hypothesized to play a role in the initiation and maintenance of estivation in snails through disturbances of acid-base status. The aim of our study was to identify the ambient CO(2) threshold that induces disturbances in acid-base status in the air-breathing land snail Helix lucorum. Acid-base parameters were determined in the hemolymph of snails acclimated to 0.5%, 1%, 2%, 4%, and 8% CO(2) in air for 20 d. In addition, we evaluated the effects of long-term acclimation on metabolic rate and on levels of D-lactate dehydrogenase activity (D-LDH) and of D-lactate in snails after 20 d of exposure to increased CO(2) levels. Helix lucorum proved to be unable to compensate for a decrease in extracellular pH (pH(e)) when acclimated to levels higher than 1% CO(2) in air. The rate of oxygen consumption started to decrease when snails were acclimated to 0.5% CO(2) in air. However, there was no correlation between the drops in pH(e) and in metabolic rate. Long-term acclimation to elevated CO(2) levels induced an increase in the activity of D-LDH with a concomitant accumulation of D-lactate in tissues. This indicates that long-term acclimation to elevated ambient CO(2) levels could reduce the aerobic capacity of land snails and trigger expression of anaerobic pathways of ATP turnover. The threshold levels of ambient CO(2) that induce changes in acid-base status and elicit metabolic depression in adult land snails H. lucorum are higher than the future atmospheric levels that are expected to result from human use of fossil energy resources.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892173"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892173"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892173; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892173]").text(description); $(".js-view-count[data-work-id=53892173]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892173; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892173']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892173, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892173]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892173,"title":"The potential role of CO2 in initiation and maintenance of estivation in the land snail Helix lucorum","translated_title":"","metadata":{"abstract":"Elevated CO(2) levels are hypothesized to play a role in the initiation and maintenance of estivation in snails through disturbances of acid-base status. The aim of our study was to identify the ambient CO(2) threshold that induces disturbances in acid-base status in the air-breathing land snail Helix lucorum. Acid-base parameters were determined in the hemolymph of snails acclimated to 0.5%, 1%, 2%, 4%, and 8% CO(2) in air for 20 d. In addition, we evaluated the effects of long-term acclimation on metabolic rate and on levels of D-lactate dehydrogenase activity (D-LDH) and of D-lactate in snails after 20 d of exposure to increased CO(2) levels. Helix lucorum proved to be unable to compensate for a decrease in extracellular pH (pH(e)) when acclimated to levels higher than 1% CO(2) in air. The rate of oxygen consumption started to decrease when snails were acclimated to 0.5% CO(2) in air. However, there was no correlation between the drops in pH(e) and in metabolic rate. Long-term acclimation to elevated CO(2) levels induced an increase in the activity of D-LDH with a concomitant accumulation of D-lactate in tissues. This indicates that long-term acclimation to elevated ambient CO(2) levels could reduce the aerobic capacity of land snails and trigger expression of anaerobic pathways of ATP turnover. The threshold levels of ambient CO(2) that induce changes in acid-base status and elicit metabolic depression in adult land snails H. lucorum are higher than the future atmospheric levels that are expected to result from human use of fossil energy resources.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Epic3physiological and Biochemical Zoology 80 Pp 113 124"},"translated_abstract":"Elevated CO(2) levels are hypothesized to play a role in the initiation and maintenance of estivation in snails through disturbances of acid-base status. The aim of our study was to identify the ambient CO(2) threshold that induces disturbances in acid-base status in the air-breathing land snail Helix lucorum. Acid-base parameters were determined in the hemolymph of snails acclimated to 0.5%, 1%, 2%, 4%, and 8% CO(2) in air for 20 d. In addition, we evaluated the effects of long-term acclimation on metabolic rate and on levels of D-lactate dehydrogenase activity (D-LDH) and of D-lactate in snails after 20 d of exposure to increased CO(2) levels. Helix lucorum proved to be unable to compensate for a decrease in extracellular pH (pH(e)) when acclimated to levels higher than 1% CO(2) in air. The rate of oxygen consumption started to decrease when snails were acclimated to 0.5% CO(2) in air. However, there was no correlation between the drops in pH(e) and in metabolic rate. Long-term acclimation to elevated CO(2) levels induced an increase in the activity of D-LDH with a concomitant accumulation of D-lactate in tissues. This indicates that long-term acclimation to elevated ambient CO(2) levels could reduce the aerobic capacity of land snails and trigger expression of anaerobic pathways of ATP turnover. 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The aim of our study was to identify the ambient CO(2) threshold that induces disturbances in acid-base status in the air-breathing land snail Helix lucorum. Acid-base parameters were determined in the hemolymph of snails acclimated to 0.5%, 1%, 2%, 4%, and 8% CO(2) in air for 20 d. In addition, we evaluated the effects of long-term acclimation on metabolic rate and on levels of D-lactate dehydrogenase activity (D-LDH) and of D-lactate in snails after 20 d of exposure to increased CO(2) levels. Helix lucorum proved to be unable to compensate for a decrease in extracellular pH (pH(e)) when acclimated to levels higher than 1% CO(2) in air. The rate of oxygen consumption started to decrease when snails were acclimated to 0.5% CO(2) in air. However, there was no correlation between the drops in pH(e) and in metabolic rate. Long-term acclimation to elevated CO(2) levels induced an increase in the activity of D-LDH with a concomitant accumulation of D-lactate in tissues. This indicates that long-term acclimation to elevated ambient CO(2) levels could reduce the aerobic capacity of land snails and trigger expression of anaerobic pathways of ATP turnover. The threshold levels of ambient CO(2) that induce changes in acid-base status and elicit metabolic depression in adult land snails H. lucorum are higher than the future atmospheric levels that are expected to result from human use of fossil energy resources.","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology"},{"id":453528,"name":"Acclimatization","url":"https://www.academia.edu/Documents/in/Acclimatization"},{"id":544188,"name":"Hemolymph","url":"https://www.academia.edu/Documents/in/Hemolymph"},{"id":1193624,"name":"Oxygen Consumption","url":"https://www.academia.edu/Documents/in/Oxygen_Consumption"},{"id":2045642,"name":"Acid-Base Equilibrium","url":"https://www.academia.edu/Documents/in/Acid-Base_Equilibrium"}],"urls":[{"id":11673948,"url":"http://epic.awi.de/16077/"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892169"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892169/Field_studies_on_the_annual_activity_and_the_metabolic_responses_of_a_land_snail_population_living_in_high_altitude"><img alt="Research paper thumbnail of Field studies on the annual activity and the metabolic responses of a land snail population living in high altitude" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892169/Field_studies_on_the_annual_activity_and_the_metabolic_responses_of_a_land_snail_population_living_in_high_altitude">Field studies on the annual activity and the metabolic responses of a land snail population living in high altitude</a></div><div class="wp-workCard_item"><span>Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and activity cycle characteristics and b) the metabolic responses of the endemic land snail species Cattania trizona olympica living at 1100m altitude in Olympus mountain (Greece). Field observations on the annual activity cycle of C. trizona olympica revealed that snails&amp;#39; activity was restricted mainly between the end of May and September, when the higher temperatures were recorded, while first matings were recorded in July and the last ones in mid September indicating a restricted favorable time period for reproduction. The activities of enzymes of intermediate metabolism showed a periodic seasonal pattern of change which seems to be closely related to the pattern of annual changes of air temperature and most of them exhibited higher activities during the coldest and warmest periods of the year. Moreover the data indicate a distinct differentiation of fuel oxidation during arousal and reproductive periods with lipid oxidation, apart from carbohydrates, contributing significantly to ATP turnover during reproductive activity. The higher enzymatic activities, determined in the tissues of C. trizona olympica than the corresponding ones determined in the tissues of the land snail species living at low altitudes, might indicate higher sensitivity of the intermediate metabolism and ATP turnover in C. trizona olympica to changes in environmental factors. Although the latter seems to be in line with the MCA hypothesis, it needs further investigation on metabolic rates to support it.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892169"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892169"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892169; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892169]").text(description); $(".js-view-count[data-work-id=53892169]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892169; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892169']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892169, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=53892169]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892169,"title":"Field studies on the annual activity and the metabolic responses of a land snail population living in high altitude","translated_title":"","metadata":{"abstract":"In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and activity cycle characteristics and b) the metabolic responses of the endemic land snail species Cattania trizona olympica living at 1100m altitude in Olympus mountain (Greece). Field observations on the annual activity cycle of C. trizona olympica revealed that snails\u0026amp;#39; activity was restricted mainly between the end of May and September, when the higher temperatures were recorded, while first matings were recorded in July and the last ones in mid September indicating a restricted favorable time period for reproduction. The activities of enzymes of intermediate metabolism showed a periodic seasonal pattern of change which seems to be closely related to the pattern of annual changes of air temperature and most of them exhibited higher activities during the coldest and warmest periods of the year. Moreover the data indicate a distinct differentiation of fuel oxidation during arousal and reproductive periods with lipid oxidation, apart from carbohydrates, contributing significantly to ATP turnover during reproductive activity. The higher enzymatic activities, determined in the tissues of C. trizona olympica than the corresponding ones determined in the tissues of the land snail species living at low altitudes, might indicate higher sensitivity of the intermediate metabolism and ATP turnover in C. trizona olympica to changes in environmental factors. Although the latter seems to be in line with the MCA hypothesis, it needs further investigation on metabolic rates to support it.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Comparative Biochemistry and Physiology Part A: Molecular \u0026 Integrative Physiology"},"translated_abstract":"In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and activity cycle characteristics and b) the metabolic responses of the endemic land snail species Cattania trizona olympica living at 1100m altitude in Olympus mountain (Greece). Field observations on the annual activity cycle of C. trizona olympica revealed that snails\u0026amp;#39; activity was restricted mainly between the end of May and September, when the higher temperatures were recorded, while first matings were recorded in July and the last ones in mid September indicating a restricted favorable time period for reproduction. The activities of enzymes of intermediate metabolism showed a periodic seasonal pattern of change which seems to be closely related to the pattern of annual changes of air temperature and most of them exhibited higher activities during the coldest and warmest periods of the year. Moreover the data indicate a distinct differentiation of fuel oxidation during arousal and reproductive periods with lipid oxidation, apart from carbohydrates, contributing significantly to ATP turnover during reproductive activity. The higher enzymatic activities, determined in the tissues of C. trizona olympica than the corresponding ones determined in the tissues of the land snail species living at low altitudes, might indicate higher sensitivity of the intermediate metabolism and ATP turnover in C. trizona olympica to changes in environmental factors. Although the latter seems to be in line with the MCA hypothesis, it needs further investigation on metabolic rates to support it.","internal_url":"https://www.academia.edu/53892169/Field_studies_on_the_annual_activity_and_the_metabolic_responses_of_a_land_snail_population_living_in_high_altitude","translated_internal_url":"","created_at":"2021-09-29T01:51:50.744-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Field_studies_on_the_annual_activity_and_the_metabolic_responses_of_a_land_snail_population_living_in_high_altitude","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"In the context of the metabolic cold adaptation hypothesis (MCA), we investigated a) the life and activity cycle characteristics and b) the metabolic responses of the endemic land snail species Cattania trizona olympica living at 1100m altitude in Olympus mountain (Greece). Field observations on the annual activity cycle of C. trizona olympica revealed that snails\u0026amp;#39; activity was restricted mainly between the end of May and September, when the higher temperatures were recorded, while first matings were recorded in July and the last ones in mid September indicating a restricted favorable time period for reproduction. The activities of enzymes of intermediate metabolism showed a periodic seasonal pattern of change which seems to be closely related to the pattern of annual changes of air temperature and most of them exhibited higher activities during the coldest and warmest periods of the year. Moreover the data indicate a distinct differentiation of fuel oxidation during arousal and reproductive periods with lipid oxidation, apart from carbohydrates, contributing significantly to ATP turnover during reproductive activity. The higher enzymatic activities, determined in the tissues of C. trizona olympica than the corresponding ones determined in the tissues of the land snail species living at low altitudes, might indicate higher sensitivity of the intermediate metabolism and ATP turnover in C. trizona olympica to changes in environmental factors. Although the latter seems to be in line with the MCA hypothesis, it needs further investigation on metabolic rates to support it.","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics"},{"id":48044,"name":"Greece","url":"https://www.academia.edu/Documents/in/Greece"},{"id":186440,"name":"Snails","url":"https://www.academia.edu/Documents/in/Snails"},{"id":577239,"name":"Altitude","url":"https://www.academia.edu/Documents/in/Altitude"},{"id":649451,"name":"Seasons","url":"https://www.academia.edu/Documents/in/Seasons"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892166"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892166/The_possible_role_of_glycolytic_enzyme_binding_in_the_control_of_glycolysis_in_Patella_caerulea_foot_muscle_during_stimulation"><img alt="Research paper thumbnail of The possible role of glycolytic enzyme binding in the control of glycolysis in Patella caerulea foot muscle during stimulation" class="work-thumbnail" src="https://attachments.academia-assets.com/70519495/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892166/The_possible_role_of_glycolytic_enzyme_binding_in_the_control_of_glycolysis_in_Patella_caerulea_foot_muscle_during_stimulation">The possible role of glycolytic enzyme binding in the control of glycolysis in Patella caerulea foot muscle during stimulation</a></div><div class="wp-workCard_item"><span>Comparative Biochemistry and Physiology Part B: Comparative Biochemistry</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The extent of glycolytic enzyme activity associated with the particulate fraction of homogenates ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The extent of glycolytic enzyme activity associated with the particulate fraction of homogenates was measured in P. caerulea foot muscle at rest and after electrical stimulation. 2. In association with the increased glycolytic rate on stimulation, there was a significant increase in the binding of certain enzymes such as phosphorylase, phosphoglucomutase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase. 3. It is concluded that the variable enzyme binding to the particulate fraction could constitute an additional mechanism for metabolic control in P. caerulea foot muscle.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6c170981d9b04a544c3840287d253072" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519495,"asset_id":53892166,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519495/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892166"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892166"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892166; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892166]").text(description); $(".js-view-count[data-work-id=53892166]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892166; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892166']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892166, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6c170981d9b04a544c3840287d253072" } } $('.js-work-strip[data-work-id=53892166]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892166,"title":"The possible role of glycolytic enzyme binding in the control of glycolysis in Patella caerulea foot muscle during stimulation","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"The extent of glycolytic enzyme activity associated with the particulate fraction of homogenates was measured in P. caerulea foot muscle at rest and after electrical stimulation. 2. 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The kinetic properties of PK from the ventricles of snails accfimated to cold for 4 days and 2 months were also determined. Short estivation (4 days) converted the PK to a less active form, possibly via enzyme phosphorylation. After 2 months of estivation, PK exhibited kinetic properties similar to control enzyme, except that the Vmx increased about 2-fold, indicating metabolic reorganization. During early and prolonged hibernation, the kinetic properties of PK did not change significantly. The results indicate that low temperature and low pH may be the main modulators of enzyme activity in the ventricle of H. iucorum during hibernation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0c92bc8b14f2d61e4bcc4046f2f6888a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519632,"asset_id":53892164,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519632/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892164"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892164"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892164; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892164]").text(description); $(".js-view-count[data-work-id=53892164]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892164; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892164']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892164, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0c92bc8b14f2d61e4bcc4046f2f6888a" } } $('.js-work-strip[data-work-id=53892164]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892164,"title":"Regulation of pyruvate kinase (PK) from the ventricle of the land snail Helix lucorum L. during early and prolonged estivation and hibernation","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"The kinetic properties of pyruvate kinase (PK) from the ventricles of early and prolonged estivated and hibernated land snails, Helix lucorum, were studied. 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The results indicate that low temperature and low pH may be the main modulators of enzyme activity in the ventricle of H. iucorum during hibernation.","publication_date":{"day":null,"month":null,"year":1994,"errors":{}},"publication_name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","grobid_abstract_attachment_id":70519632},"translated_abstract":null,"internal_url":"https://www.academia.edu/53892164/Regulation_of_pyruvate_kinase_PK_from_the_ventricle_of_the_land_snail_Helix_lucorum_L_during_early_and_prolonged_estivation_and_hibernation","translated_internal_url":"","created_at":"2021-09-29T01:51:50.537-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":30238162,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":70519632,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/70519632/thumbnails/1.jpg","file_name":"0305-0491_2894_2990189-920210929-12269-1s9w8qd.pdf","download_url":"https://www.academia.edu/attachments/70519632/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Regulation_of_pyruvate_kinase_PK_from_th.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/70519632/0305-0491_2894_2990189-920210929-12269-1s9w8qd-libre.pdf?1632906053=\u0026response-content-disposition=attachment%3B+filename%3DRegulation_of_pyruvate_kinase_PK_from_th.pdf\u0026Expires=1733952068\u0026Signature=E6FYQgpcW4oCKjKgU73zQ4Uu0Cp39ucrx0aja3oHpqnElnsYOr7pmcdNXCMCQjnDPHydsLq9nKfssVpE4WHk0K4dEiBSbJD-xBHX7G2sbwLfk4Zi9QRLaxRYVJkE0kJuVGJ4VlzCrzcAvNf1NZ~9Wbdld8ZveBY5FD1Fozjcg1ZFD0uvHmnJiqI8tOB00ouk0ycIOwlmunAJ27F~lGJjqHhOqd3DphKa0ajTuj4wNpzKVGQEtB-mlcpsdqwziZWes~ad7IBzlSh0hhcVfFQxfG64SM7a-yvbdHc-3puAU03smrB-uSR6uhIN8FjwT6zg2moWYZ4CjnZFk1PLnwD3pw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Regulation_of_pyruvate_kinase_PK_from_the_ventricle_of_the_land_snail_Helix_lucorum_L_during_early_and_prolonged_estivation_and_hibernation","translated_slug":"","page_count":7,"language":"en","content_type":"Work","summary":"The kinetic properties of pyruvate kinase (PK) from the ventricles of early and prolonged estivated and hibernated land snails, Helix lucorum, were studied. The kinetic properties of PK from the ventricles of snails accfimated to cold for 4 days and 2 months were also determined. Short estivation (4 days) converted the PK to a less active form, possibly via enzyme phosphorylation. After 2 months of estivation, PK exhibited kinetic properties similar to control enzyme, except that the Vmx increased about 2-fold, indicating metabolic reorganization. During early and prolonged hibernation, the kinetic properties of PK did not change significantly. The results indicate that low temperature and low pH may be the main modulators of enzyme activity in the ventricle of H. iucorum during hibernation.","owner":{"id":30238162,"first_name":"Basile","middle_initials":null,"last_name":"Michaelidis","page_name":"BasileMichaelidis","domain_name":"independent","created_at":"2015-04-24T13:45:35.376-07:00","display_name":"Basile Michaelidis","url":"https://independent.academia.edu/BasileMichaelidis"},"attachments":[{"id":70519632,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/70519632/thumbnails/1.jpg","file_name":"0305-0491_2894_2990189-920210929-12269-1s9w8qd.pdf","download_url":"https://www.academia.edu/attachments/70519632/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Regulation_of_pyruvate_kinase_PK_from_th.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/70519632/0305-0491_2894_2990189-920210929-12269-1s9w8qd-libre.pdf?1632906053=\u0026response-content-disposition=attachment%3B+filename%3DRegulation_of_pyruvate_kinase_PK_from_th.pdf\u0026Expires=1733952068\u0026Signature=E6FYQgpcW4oCKjKgU73zQ4Uu0Cp39ucrx0aja3oHpqnElnsYOr7pmcdNXCMCQjnDPHydsLq9nKfssVpE4WHk0K4dEiBSbJD-xBHX7G2sbwLfk4Zi9QRLaxRYVJkE0kJuVGJ4VlzCrzcAvNf1NZ~9Wbdld8ZveBY5FD1Fozjcg1ZFD0uvHmnJiqI8tOB00ouk0ycIOwlmunAJ27F~lGJjqHhOqd3DphKa0ajTuj4wNpzKVGQEtB-mlcpsdqwziZWes~ad7IBzlSh0hhcVfFQxfG64SM7a-yvbdHc-3puAU03smrB-uSR6uhIN8FjwT6zg2moWYZ4CjnZFk1PLnwD3pw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics"},{"id":11035,"name":"Regulation","url":"https://www.academia.edu/Documents/in/Regulation"},{"id":45304,"name":"Hibernation","url":"https://www.academia.edu/Documents/in/Hibernation"},{"id":71434,"name":"Pyruvate Kinase","url":"https://www.academia.edu/Documents/in/Pyruvate_Kinase"},{"id":172083,"name":"Phosphorylation","url":"https://www.academia.edu/Documents/in/Phosphorylation"},{"id":172897,"name":"Glycolysis","url":"https://www.academia.edu/Documents/in/Glycolysis"},{"id":395059,"name":"Land snail","url":"https://www.academia.edu/Documents/in/Land_snail"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="53892161"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53892161/Studies_on_the_anaerobic_energy_metabolism_in_the_foot_muscle_of_marine_gastropod_Patella_caerulea_L_"><img alt="Research paper thumbnail of Studies on the anaerobic energy metabolism in the foot muscle of marine gastropod Patella caerulea (L.)" class="work-thumbnail" src="https://attachments.academia-assets.com/70519633/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53892161/Studies_on_the_anaerobic_energy_metabolism_in_the_foot_muscle_of_marine_gastropod_Patella_caerulea_L_">Studies on the anaerobic energy metabolism in the foot muscle of marine gastropod Patella caerulea (L.)</a></div><div class="wp-workCard_item"><span>Comparative Biochemistry and Physiology Part B: Comparative Biochemistry</span><span>, 1990</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The foot muscle of P. caerulea has a complete sequence of glycolytic enzymes. The low activity of...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The foot muscle of P. caerulea has a complete sequence of glycolytic enzymes. The low activity of hexokinase, in comparison with the activities of glycogen phosphorylase and phosphofructokinase, indicate that glycogen is the main fuel oxidized. 2. The reduction of aspartate content in combination with the accumulation of alanine and the presence of considerable activities of glutamate-oxaloacetate transaminase and glutamate~pyruvate transaminase indicates a coupled metabolism of glycogen and aspartate during exposure to air. 3. From the changes in the concentration of the metabolites during exposure to air it appears that up to the second hour of anaerobiosis alanine, lactate and glutamate are the end-products which accumulate in the foot muscle of P. caerulea, whereas from the second to the fourth hour only succinate and alanine accumulate. 4. The low activities of the Krebs cycle enzymes as well as the absence of ~t-ketoglutarate dehydrogenase activity suggest that the Krebs cycle is not in operation. 5. The absence of opine dehydrogenases shows that the end products octopine, alanopine and strombine are not accumulated in the foot muscle under anaerobiosis. 6. The high activity of malate dehydrogenase in the direction of malate formation in combination with the low activity of ~-lactate dehydrogenase and the absence of opine dehydrogenases suggests that the former dehydrogenase is coupled 1 : 1 to glyceraldehyde-3-phosphate dehydrogenase.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="203a24af0561af2c72c4900c9a519766" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70519633,"asset_id":53892161,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70519633/download_file?st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&st=MTczMzk0ODQ2OCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53892161"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53892161"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53892161; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53892161]").text(description); $(".js-view-count[data-work-id=53892161]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53892161; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='53892161']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 53892161, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "203a24af0561af2c72c4900c9a519766" } } $('.js-work-strip[data-work-id=53892161]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":53892161,"title":"Studies on the anaerobic energy metabolism in the foot muscle of marine gastropod Patella caerulea (L.)","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"The foot muscle of P. caerulea has a complete sequence of glycolytic enzymes. The low activity of hexokinase, in comparison with the activities of glycogen phosphorylase and phosphofructokinase, indicate that glycogen is the main fuel oxidized. 2. The reduction of aspartate content in combination with the accumulation of alanine and the presence of considerable activities of glutamate-oxaloacetate transaminase and glutamate~pyruvate transaminase indicates a coupled metabolism of glycogen and aspartate during exposure to air. 3. From the changes in the concentration of the metabolites during exposure to air it appears that up to the second hour of anaerobiosis alanine, lactate and glutamate are the end-products which accumulate in the foot muscle of P. caerulea, whereas from the second to the fourth hour only succinate and alanine accumulate. 4. The low activities of the Krebs cycle enzymes as well as the absence of ~t-ketoglutarate dehydrogenase activity suggest that the Krebs cycle is not in operation. 5. The absence of opine dehydrogenases shows that the end products octopine, alanopine and strombine are not accumulated in the foot muscle under anaerobiosis. 6. 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