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class="clearfix page-start"></div> <a name="main-content"></a> <div class="system-messages clearfix"> <div class="container"> <div class="row"> <div class="col-md-12"> <div class="region region-system-messages"> <div data-drupal-messages-fallback class="hidden"></div> <div id="block-showcase-lite-page-title" class="clearfix block block-core block-page-title-block"> <div class="content"> <h1 class="title page-title"><span class="field field--name-title field--type-string field--label-hidden">Stem Cell Core</span> </h1> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="clearfix main-content region--dark-typography region--white-background region--no-separator"> <div class="landing-page-container" > <div class="clearfix main-content__container"> <div class="row"> <section> <div class="clearfix main-content__section" > <div class="region region-content"> <div id="block-showcase-lite-content" class="clearfix block block-system block-system-main-block"> <div class="content"> <article data-history-node-id="3273" class="node node--type-core-mini-sites node--promoted node--view-mode-full clearfix"> <div class="node__container"> <div class="node__main-content clearfix"> <header class="node__header"> </header> <div id="mini-core-tabs" class="row"> <div id="subtabs" class="subtabs"> <ul class="sudonav"> <li class="tab"><a href="#overview"><span>Overview</span></a></li> <li class="tab"><a href="#services"><span>Services</span></a></li> <li class="tab"><a href="#equipment"><span>Equipment</span></a></li> <li class="tab"><a href="#fees-and-scheduling"><span>Fees and Scheduling</span></a></li> <li class="tab"><a href="#publications"><span>Publications</span></a></li> <li class="tab"><a href="#faqs"><span>Faqs</span></a></li> </ul> <div class="content-wrap"> <section id="overview"> <div id="intro"> <div class="container"> <p class="large">The Stem Cell Core offers a fully functional tissue culture facility with specialized instruments, media, reagents, cell lines, and expertise for new or established researchers to perform cutting-edge stem cell research. In addition, our core provides comprehensive human induced pluripotent stem cells (iPS cells)–related training, consultations, and standard and customized scientific services, including derivation of iPS cells, differentiation of iPS cells and genome editing in iPS cells to stem cell researchers.</p> </div> </div> <div id="manager"> <div class="container"> <h2>Contact</h2> <div class="user__compact"> <p><b>Stem Cell Core Staff</b><br> <a href="mailto:stemcell-core@gladstone.ucsf.edu" style="font-weight:600; font-size:24px">Email</a> <br> </p> </div> </div> </div> <div class="bk1">   </div> <div class="services"> <div class="container"> <h2>Services Provided</h2> <ul> <li>iPS cell reprogramming from human fibroblasts and blood</li> <li>Peripheral Blood Mononuclear Cells (PBMCs) isolated from whole human blood</li> <li>CRISPR-mediated gene editing in human iPS cells</li> <li>Differentiation of human iPS cells into cardiomyocytes and cerebral organoids</li> <li>Transcription factor-mediated differentiation of human iPS cells into neurons</li> <li>Cell banking and distribution of human iPS cells and skin fibroblasts</li> <li>Training in human iPS cell culture, reprogramming and cardiac differentiation</li> <li>Standard quality control (mycoplasma screening, pluripotency, genetic stability)</li> <li>Consultations, collaborations, and educational seminars</li> </ul> <p><a class="button magenta ov-svcs" href="#services">Learn More about Our Services</a></p> </div> </div> <div class="core-equipment"> <div class="container"> <h2>Core Equipment</h2> <div class="views-element-container"><div class="view view-equipment view-id-equipment view-display-id-equip js-view-dom-id-e5a2214a6bda8371e34f4743552748d13a14dc608fccae24865fbcda907271e4"> <div class="view-header"> <style> .view-display-id-confocal #3040 {visibility:hidden !important} </style> </div> <div class="view-content"> <div class="views-view-grid horizontal cols-2 clearfix"> <div class="views-row clearfix row-1"> <div class="views-col col-1" style="width: 50%;"><div class="views-field views-field-title"><span class="field-content"><p>IncuCyte S3</p></span></div></div> <div class="views-col col-2" style="width: 50%;"><div class="views-field views-field-title"><span class="field-content"><p>EVOS FL Core Imaging System </p></span></div></div> </div> <div class="views-row clearfix row-2"> <div class="views-col col-1" style="width: 50%;"><div class="views-field views-field-title"><span class="field-content"><p>Maestro Edge</p></span></div></div> <div class="views-col col-2" style="width: 50%;"><div class="views-field views-field-title"><span class="field-content"><p>Hana Single Cell Dispenser</p></span></div></div> </div> </div> </div> </div> </div> <p><a class="button magenta" href="#equipment">Learn more about Our Equipment</a></p> </div> </div> <div class="core-members"> <div class="container"> <h2>Core Members</h2> <div class="views-element-container"><div class="view view-user-admin-people view-id-user_admin_people view-display-id-core_members js-view-dom-id-7f52460a8f136229b68e6cfc168c96c019b79a26193f8040cff941dd41ce6909"> <div class="view-content"> <div class="views-view-grid horizontal cols-2 clearfix"> <div class="views-row clearfix row-1"> <div class="views-col col-1" style="width: 50%;"><article class="user user--compnomail"> <div class="user__compact"> <p><b>Sam Hu</b><br>Research Technologist I<br></p> </div> </article> </div> <div class="views-col col-2" style="width: 50%;"><article class="user user--compnomail"> <div class="user__compact"> <p><b>Ritu Kumar</b><br>Core Director<br></p> </div> </article> </div> </div> <div class="views-row clearfix row-2"> <div class="views-col col-1" style="width: 50%;"><article class="user user--compnomail"> <div class="user__compact"> <p><b>Wendy Runyon</b><br>Research Scientist<br></p> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> <div id="events"> <div class="container"> <p class="large">The Stem Cell Core hosts a number of events from training to product demos.</p> <p class="butncenter"><a class="button magenta" href="/events?search=stem+cell+core">Upcoming Events</a></p> </div> </div> <div id="related-news"> <div class="container"> <h2>Related News</h2> <div class="owl-carousel" id="news-carousel"> <a href="/news/gladstone-expand-stem-cell-research-capabilities-multi-million-dollar-regenerative-medicine" title="Read more" target="_self"><div class="item"><img alt="Sam Hu prepares induced pluripotent stem cells." class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/_aaa1066_1.jpg?itok=IrGOuDA_ " /><div class="blowpic">Gladstone to Expand Stem Cell Research Capabilities with Multi-Million Dollar Regenerative Medicine Grant<br><div class="summy">Funds from the California Institute for Regenerative Medicine will allow Gladstone to bolster its disease-modeling technologies, add robust CRISPR screening capabilities, and educate young…</div><div class="authored"><time datetime="2024-03-11T09:34:32-07:00" class="datetime">March 11, 2024</time> </div></div></div></a><a href="/news/meet-gladstone-ritu-kumar" title="Read more" target="_self"><div class="item"><img alt="GIF of interview of Ritu Kumar, director of the Stem Cell Core at Gladstone" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/meet-gladstone-ritu-kumar-hero.gif?itok=OarhYDey " /><div class="blowpic">Meet Gladstone: Ritu Kumar<br><div class="summy">Ritu Kumar, director of the Stem Cell Core, describes her research at Gladstone, the women who have mentored her throughout her career, and the changes she'd like to see for women in STEM</div><div class="authored"><time datetime="2023-03-27T13:26:10-07:00" class="datetime">March 27, 2023</time> </div></div></div></a><a href="/news/15-years-later-how-scientists-are-using-induced-pluripotent-stem-cells" title="Read more" target="_self"><div class="item"><img alt="Graphic representing induced pluripotent stem cells (iPS cells)" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news/ipsc_anniversary_hero.jpeg?itok=_fnvTMjH " /><div class="blowpic">15 Years Later: This Is How Scientists Are Using Induced Pluripotent Stem Cells<br><div class="summy">Gladstone researchers talk about the power of iPS cells in biomedical research</div><div class="authored"><time datetime="2022-11-22T10:09:44-08:00" class="datetime">November 22, 2022</time> </div></div></div></a><a href="https://www.mercurynews.com/2022/03/08/disease-in-a-dish-what-mini-hearts-and-other-organs-reveal-about-long-covid/" title="Read more" target="_blank"><div class="item"><img alt="Feature image" class="owl-lazy" data-src="/themes/contrib/showcase_lite/images/random/Share-Image1.jpg" /><div class="blowpic">Disease in a Dish: What Mini-Hearts and Other Organs Reveal about Long COVID<br><div class="summy"><b>San Jose Mercury News</b>—Senior Investigators Melanie Ott, MD, PhD, and Bruce Conklin, MD, recount how they used miniature balls of beating heart cells called organoids to study the effects of COVID-19 on the heart. The…</div><div class="authored"><time datetime="2022-03-09T16:25:34-08:00" class="datetime">March 9, 2022</time> </div></div></div></a><a href="/news/machine-meet-stem-cells" title="Read more" target="_self"><div class="item"><img alt="Todd McDevitt and Ashley Libby standing behind David Joy who is working on a computer in the lab" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/AshleyBruceDavid.jpg?itok=ycr4Rp_m " /><div class="blowpic">Machine, Meet Stem Cells<br><div class="summy">Gladstone researchers used a machine-learning approach to discover new ways of controlling the spatial organization of induced pluripotent stem cells.</div><div class="authored"><time datetime="2019-11-20T00:00:00-08:00" class="datetime">November 20, 2019</time> </div></div></div></a><a href="/news/connecting-dots-stem-cells-human-health" title="Read more" target="_self"><div class="item"><img alt="Illustration of stem cells" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/StemCellStory-banner.jpg?itok=tKFYuOIL " /><div class="blowpic">Connecting the Dots: From Stem Cells to Human Health<br><div class="summy">Since Shinya Yamanaka discovered a method to create human induced pluripotent stem (iPS) cells, Gladstone researchers have built on this technology to create numerous iPS cell lines, coax iPS cells…</div><div class="authored"><time datetime="2019-10-08T20:03:00-07:00" class="datetime">October 8, 2019</time> </div></div></div></a><a href="/news/meet-gladstone-wendy-runyon" title="Read more" target="_self"><div class="item"><img alt="Film strip of Wendy Runyon" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/Wendy-Runyon-BW_Strip_0.jpg?itok=siVMGunY " /><div class="blowpic">Meet Gladstone: Wendy Runyon<br><div class="summy">Wendy explains how being rejected for a lab position led her to work in what she describes as “zookeeping for cells.” She also talks about her Masters Swimming skills and how she developed her secret…</div><div class="authored"><time datetime="2018-03-30T12:03:00-07:00" class="datetime">March 30, 2018</time> </div></div></div></a> </div> </div> </div> </section> <section id="services"> <div class="container"> <h2 class="tabtop">Services Provided</h2> <p>The Stem Cell Core specializes in the generation, maintenance and differentiation of human iPS cells. The core has expertise in human iPS cell reprogramming and CRISPR-Cas9 genome editing of human iPS cells. The core supports researchers by providing the physical space, validated reagents, iPS cell lines and hands-on training for scientists to use iPS cells for their research goals.</p> <div class="alternator"> <div class="row"> <div class="col-sm-6"> <h3>Human iPS Cells Reprogramming</h3> <ul> <li>iPS Cell Reprogramming from Human Peripheral Blood Mononuclear Cells (PBMCs) (Episomal method)</li> <li>iPS Cell Reprogramming from Fibroblasts (ReproRNA StemCell Technologies Kit)</li> </ul> </div> <div class="col-sm-6"> <img alt="iPS Cell Reprogramming" src="/sites/default/files/inline-images/Human%20iPS%20Cells%20Reprogramming.jpg" /> </div> </div> <div class="row"> <div class="col-sm-6"> <img alt="Cardiomyocyte Differentation" src="/sites/default/files/inline-images/Cardiomyocyte%20differentiation%20from%20human%20iPS%20Cells.jpg" /> </div> <div class="col-sm-6"> <h3>Cardiomyocyte Differentiation from Human iPS Cells</h3> <ul> <li>Modulating Wnt Signaling</li> <li>StemCell Technologies Kit</li> </ul> </div> </div> <div class="row"> <div class="col-sm-6"> <h3>Cerebral Organoid Differentiation from Human iPS Cells</h3> <ul> <li>StemCell Technologies Kit</li> </ul> </div> <div class="col-sm-6"> <img alt="Cerebral Organid Differentiation" src="/sites/default/files/inline-images/Cerebral%20organid%20differentiation%20from%20Human%20iPS%20Cells.jpg" /> </div> </div> <div class="row"> <div class="col-sm-6"> <img alt="Transcription Factor" src="/sites/default/files/inline-images/Transcription%20factor%20mediated%20differentiation%20of%20iPSCs.jpg" /> </div> <div class="col-sm-6"> <h3>Transcription Factor Mediated Differentiation of Human iPS Cells</h3> <ul> <li>Cortical Neurons (by Inducing NGN2)</li> <li>Motor Neurons (by Inducing ISL1, LHX3 and NGN2)</li> </ul> </div> </div> <div class="row"> <div class="col-sm-6"> <h3>Genome Engineering of Human iPS Cells</h3> <ul> <li>CRISPR-Cas9 Gene Knockout by NHEJ</li> <li>CRISPR-Cas9 Gene Knock-in by HDR</li> </ul> </div> <div class="col-sm-6"> <img alt="Genome Engineering" src="/sites/default/files/inline-images/Genome%20engineering%20of%20human%20iPS%20Cells.jpg" /> </div></div> <div class="row"> <div class="col-sm-6"> <img alt="Training" data-entity-type="file" src="/sites/default/files/inline-images/Training.jpg" /> </div> <div class="col-sm-6"> <h3>Training Services</h3> <ul> <li>Human iPS Cells Culture and Maintenance</li> <li>Cardiomyocyte Differentiation from Human iPS Cells</li> <li>Human iPS Cell Reprogramming from Fibroblasts and PBMCs</li> </ul> </div> </div> <div class="row"> <div class="col-sm-6"> <h3>Supply of Cell Lines</h3> <ul> <li>WTC-iPSC</li> <li>WTB-iPSC</li> <li>CRISPRi-iPSC</li> <li>CRISPRn-iPSC</li> <li>GCaMP6f-iPCS</li> </ul> </div> <div class="col-sm-6"> <img alt="Supply" data-entity-type="file" src="/sites/default/files/inline-images/Supply-Cell-Lines.jpg" /> </div></div> </div> <style> .alternator {margin-top:-72px} .alternator .row{margin:114px 0} .alternator img {max-width:100%} </style> </div> <div class="bk2">   </div> </section> <section id="equipment"> <div id="equip-intro" class="container"> <h2 class="tabtop">Instrumentation</h2> </div> <div id="equip-list"> <div class="views-element-container"><div class="view view-equipment view-id-equipment view-display-id-stem_cell js-view-dom-id-143a5e683535480ef7e6ea45a94427a37b1e443c8ce0287d527285986e6ee88b"> <div class="view-header"> <style> .view-display-id-confocal #3040 {visibility:hidden !important} </style> </div> <div class="view-content"> <div class="flow-5723 views-row"><div class="views-field views-field-field-description"><div class="field-content"></div></div></div> <div class="flow-5726 views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2023-09/_DSC9181-evos-FL-Core.jpg" /> </div> <div class="flowtext"> <h4>EVOS FL Core Imaging System </h4> <p>The EVOS FL is a comprehensive, high-performance fluorescence microscope crafted for both efficiency and user-friendliness. Its integrated onboard computer and imaging software enables users to directly capture, manage, and store multichannel fluorescence images and data from the microscope. This microscope is housed within a biosafety cabinet, making it well-suited for the picking of iPSC/ES colonies.</p> <p id="manu-butt"><a target="_blank" href="https://www.fishersci.com/shop/products/evos-fl-digital-inverted-fluorescence-microscope/12563460" class="button outline">Manufacturer Site</a></p> </div> </div> </div></div></div> <div class="flow-5724 views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2023-09/_DSC9527-maestro-edge.jpg" /> </div> <div class="flowtext"> <h4>Maestro Edge</h4> <p>Maestro Edge MEA and Impedance system is ideal for low-throughput experiments. Record from 6- and 24-well MEA plates, plus 96-well impedance plates. Explore cell functionality easily in multiwell setups, whether tracking electrical activity or cell growth.</p> <p id="manu-butt"><a target="_blank" href="https://www.axionbiosystems.com/products/mea/maestro-edge" class="button outline">Manufacturer Site</a></p> </div> </div> </div></div></div> <div class="flow-5748 views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2023-09/_DSC9364-hana-single-cell-dispenser.jpg" /> </div> <div class="flowtext"> <h4>Hana Single Cell Dispenser</h4> <p>The Hana Single Cell Dispenser makes single cell isolation and sorting effortless. This benchtop instrument uses proprietary microfluidics technology that combines flow cytometry and liquid dispensing to sort and dispense single cells directly into 96-well or 384-well plate. It simplifies and empowers a number of single cell applications, including cell line development and engineering, single cell genomics, iPSC and CRISPR cloning, antibody discovery, single cell mass spec, synthetic biology, and rare cell isolation such as circulating tumor cells (CTCs) and circulating fetal cells.</p> <p id="manu-butt"><a target="_blank" href="https://www.namocell.com/hana-single-cell-dispenser/" class="button outline">Manufacturer Site</a></p> </div> </div> </div></div></div> </div> <div class="view-footer"> <style> .flow-7270{padding-top:72px;padding-bottom: 40px} .flow- { padding-top: 72px;padding-bottom: 30px} .flow- #manu-butt{margin-top:25px !important} </style> </div> </div> </div> </div> <div class="bk3">   </div> </section> <section id="fees-and-scheduling"> <div class="container"> <h2 class="tabtop">Fees and Scheduling</h2> <ul><li>Use of the Stem Cell Core facility is subject to fees charged as an add-on cost to in-house media purchased; alternatively, fees are charged via the purchase of a “sticker”. The add-on cost covers the use of plastics, PPE, some commonly used reagents, and standard equipment usage.</li><li>Costs/fees for media, reagents, scientific services, and specialized equipment usage are listed on iLab. An iLab account is required to view specific pricing.</li><li>The first 30-minute consultation is free of charge.</li><li>For all fee inquiries or to set up an iLab account, <a href=" mailto:stemcell-core@gladstone.ucsf.edu">email stemcell-core@gladstone.ucsf.edu.</a></li><li>For custom scientific project inquiries or inquiries about use of specialized instruments, <a href="mailto:stemcell-core@gladstone.ucsf.edu">email stemcell-core@gladstone.ucsf.edu.</a></li></ul><h2>Scheduling</h2><ul><li>To schedule new consultations and service requests, <a href="mailto:stemcell-core@gladstone.ucsf.edu">contact stemcell-core@gladstone.ucsf.edu.</a> Once accepted, log into your Stem Cell Core iLab account and request the service.</li><li>To schedule equipment use, visit iLab and book in the calendar.</li></ul> </div> <div class="bk4">   </div> </section> <section id="publications"> <div class="container"> <h2 class="tabtop">Publications</h2> <p><a href="https://pubmed.ncbi.nlm.nih.gov/35182466/" target="_blank" rel="noopener">Transcription Factor Protein Interactomes Reveal Genetic Determinants in Heart Disease.</a> Barbara Gonzalez-Teran , Maureen Pittman , Franco Felix , Reuben Thomas , Desmond Richmond-Buccola , Ruth Hüttenhain , Krishna Choudhary , Elisabetta Moroni , Mauro W Costa , Yu Huang , Arun Padmanabhan , Michael Alexanian , Clara Youngna Lee , Bonnie E J Maven , Kaitlen Samse-Knapp , Sarah U Morton , Michael McGregor , Casey A Gifford , J G Seidman , Christine E Seidman , Bruce D Gelb , Giorgio Colombo , Bruce R Conklin , Brian L Black , Benoit G Bruneau , Nevan J Krogan , Katherine S Pollard , Deepak Srivastava Cell 2022 Mar 3;185(5):794-814.</p><p><a href="https://pubmed.ncbi.nlm.nih.gov/35082446/" target="_blank" rel="noopener">Brahma Safeguards Canalization of Cardiac Mesoderm Differentiation.</a> Swetansu K Hota , Kavitha S Rao , Andrew P Blair , Ali Khalilimeybodi , Kevin M Hu , Reuben Thomas , Kevin So , Vasumathi Kameswaran , Jiewei Xu , Benjamin J Polacco , Ravi V Desai , Nilanjana Chatterjee , Austin Hsu , Jonathon M Muncie , Aaron M Blotnick , Sarah A B Winchester , Leor S Weinberger , Ruth Hüttenhain , Irfan S Kathiriya , Nevan J Krogan , Jeffrey J Saucerman , Benoit G Bruneau. Nature 2022 Feb;602(7895):129-134.</p><p><a href="https://pubmed.ncbi.nlm.nih.gov/35938400/" target="_blank" rel="noopener">Transcription Factor GATA4 Regulates Cell Type-Specific Splicing Through Direct Interaction With RNA in Human Induced Pluripotent Stem Cell-Derived Cardiac Progenitors.</a> Lili Zhu , Krishna Choudhary , Barbara Gonzalez-Teran , Yen-Sin Ang , Reuben Thomas Nicole R Stone , Lei Liu , Ping Zhou , Chenchen Zhu , Hongmei Ruan , Yu Huang , Shibo Jin, Angelo Pelonero , Frances Koback , Arun Padmanabhan , Nandhini Sadagopan , Austin Hsu , Mauro W Costa , Casey A Gifford , Joke G van Bemmel , Ruth Hüttenhain , Vasanth Vedantham , Bruce R Conklin , Brian L Black , Benoit G Bruneau , Lars Steinmetz , Nevan J Krogan , Katherine S Pollard , Deepak Srivastava. Circulation 2022 Sep 6;146(10):770-787.</p><p><a href="https://pubmed.ncbi.nlm.nih.gov/33723017/" target="_blank" rel="noopener">Sars-Cov-2 Infection Of Human Ipsc-Derived Cardiac Cells Reflects Cytopathic Features in Hearts of Patients with Covid-19.</a> Perez-Bermejo JA, Kang S, Rockwood SJ, Simoneau CR, Joy DA, Silva AC, Ramadoss GN, Flanigan WR, Fozouni P, Li H, Chen PY, Nakamura K, Whitman JD, Hanson PJ, McManus BM, Ott M, Conklin BR, McDevitt TC. Sci Transl Med 2021 Apr 21;13(590).</p><p><a href="https://pubmed.ncbi.nlm.nih.gov/34485306/">Allele-Specific Gene Editing Rescues Pathology in a Human Model of Charcot-Marie-Tooth Disease Type 2E.</a> Feliciano CM, Wu K, Watry HL, Marley CBE, Ramadoss GN, Ghanim HY, Liu AZ, Zholudeva LV, McDevitt TC, Saporta MA, Conklin BR, Judge LM. Front Cell Dev Biol 2021 Aug 16;9:723023.</p><p><a href="https://pubmed.ncbi.nlm.nih.gov/34087997/" target="_blank" rel="noopener">Transcription Factor Overexpression Drives Reliable Differentiation of Retinal Pigment Epithelium From Human Induced Pluripotent Stem Cells.</a> Dewell TE, Gjoni K, Liu AZ, Libby ARG, Moore AT, So PL, Conklin BR. Stem Cell Res 2021 May;53:102368.</p><p><a href="https://pubmed.ncbi.nlm.nih.gov/31722212/" target="_blank" rel="noopener">Sox2 and Klf4 as the Functional Core in Pluripotency Induction without Exogenous Oct4.</a> An, Z., Liu, P., Zheng, J., et al. 2019. <em>Cell Reports</em> 29(7), p. 1986–2000.e8.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5441696/" target="_blank" rel="noopener">Differentiation of V2a interneurons from human pluripotent stem cells.</a> Butts, J.C., McCreedy, D.A., Martinez-Vargas, J.A., et al. 2017. <em>Proceedings of the National Academy of Sciences of the United States of America</em> 114(19), pp. 4969–4974.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557373/" target="_blank" rel="noopener">Oligogenic inheritance of a human heart disease involving a genetic modifier. </a>Gifford, C.A., Ranade, S.S., Samarakoon, R., et al. 2019. <em>Science</em> 364(6443), pp. 865–870.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5135304/" target="_blank" rel="noopener">BMP-SMAD-ID promotes reprogramming to pluripotency by inhibiting p16/INK4A-dependent senescence.</a> Hayashi, Y., Hsiao, E.C., Sami, S., et al. 2016. <em>Proceedings of the National Academy of Sciences of the United States of America</em> 113(46), pp. 13057–13062.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837370/" target="_blank" rel="noopener">Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses.</a> Huebsch, N., Loskill, P., Deveshwar, N., et al. 2016. <em>Scientific Reports</em> 6, p. 24726.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410286/" target="_blank" rel="noopener">Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales.</a> Huebsch, N., Loskill, P., Mandegar, M.A., et al. 2015. Tissue Engineering. Part C, <em>Methods</em> 21(5), pp. 467–479.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518554/" target="_blank" rel="noopener">A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress.</a> Judge, L.M., Perez-Bermejo, J.A., Truong, A., et al. 2017. <em>Journal of Clinical Investigation Insight</em> 2(14). PMC5518554</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177255/" target="_blank" rel="noopener">Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference.</a> Libby, A.R., Joy, D.A., So, P.-L., et al. 2018. <em>eLife</em> 7.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830697/" target="_blank" rel="noopener">CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.</a> Mandegar, M.A., Huebsch, N., Frolov, E.B., et al. 2016. <em>Cell Stem Cell</em> 18(4), pp. 541–553.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063274/" target="_blank" rel="noopener">Isolation of single-base genome-edited human iPS cells without antibiotic selection.</a> Miyaoka, Y., Chan, A.H., Judge, L.M., et al. 2014. <em>Nature Methods</em> 11(3), pp. 291–293.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589096/" target="_blank" rel="noopener">Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq.</a> Wienert, B., Wyman, S.K., Richardson, C.D., et al. 2019. <em>Science</em> 364(6437), pp. 286–289.</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222128/ " target="_blank" rel="noopener">The Cellular NMD Pathway Restricts Zika Virus Infection and Is Targeted by the Viral Capsid Protein.</a> Fontaine, K.A., Leon, K.E., Khalid, M.M, Tomar, S., Jimenez-Morales, D., Dunlap, M., Kaye, J.A., Shah, P.S., Finkbeiner, S., Krogan, N.J., Ott. M. 2018. <em>mBio</em>. Nov-Dec; 9(6) PMCID: PMC6222128</p><p><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726454/" target="_blank" rel="noopener">Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells.</a> Kime, C., Mandegar, M. A., Srivastava, D., Yamanaka, S., Conklin, B. R., & Rand, T. A. (2016). <em>Current Protocols in Human Genetics</em>, 88, 21.4.1–21.4.23.</p><p><a href="https://doi.org/10.1016/j.cell.2015.02.035" target="_blank" rel="noopener">Human disease modeling reveals integrated transcriptional and epigenetic mechanisms of NOTCH1 haploinsufficiency.</a> Theodoris, C. V., Li, M., White, M. P., Liu, L., He, D., Pollard, K. S., Bruneau, B. G., & Srivastava, D. (2015). <em>Cell</em>, 160(6), 1072–1086.</p> <p class="cntrbutt"><a class="morepub button outline">More Publications</a></p> </div> <div class="bk5">   </div> </section> <section id="faqs"> <div class="accordion-wrapper"> <div class="container"> <h2>FAQs</h2> <div class="accordion accord1"><h3>I would like to use the Stem Cell Core facility. How do I get started?</h3></div> <div class="panel"> <p>Contact us at <a href="mailto:stemcell-core@gladstone.ucsf.edu">stemcell-core@gladstone.ucsf.edu.</a></p> </div> <div class="accordion"><h3>Do I Need a Service Contract for the Services?</h3></div> <div class="panel"> <p>All non-Gladstone investigators need a service contract for the services provided by the Stem Cell Core.</p> </div> <div class="accordion"><h3>How Do I Pay For Services?</h3></div> <div class="panel"> <p>Consumables, services, and instrument usage are paid via iLab. We would set up an iLab account for you and you would submit a fund number (PO number) to order through your account.</p> </div> <div class="accordion"><h3>Do You Provide Customized Services or Collaborations? </h3></div> <div class="panel"> <p>Yes, email <a href="mailto:stemcell-core@gladstone.ucsf.edu">stemcell-core@gladstone.ucsf.edu</a> to discuss your project.</p> </div> <div class="accordion"><h3>What Is the Wait Time for Scheduling My Project? </h3></div> <div class="panel"> <p>It depends on the project request and current workload of the Stem Cell Core. We generally require at least 2 weeks' notice to schedule new projects. 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