CINXE.COM

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class="ds_title">Human Ghrelin DuoSet ELISA </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: DY8149-05</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 4 Citations </a> </span> <a href="/reviews/DY8149-05" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/human-ghrelin-duoset-elisa_dy8149-05" method="post" id="atc-datasheet-form" accept-charset="UTF-8"><div><div id="atc_datasheet_block">     <table class="add_to_cart"> <thead> <tr> <th class="ds_catalog">Catalog #</th> <th 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class="btn btn-secondary atc_request_quote_simple_plex_assay" style="display:none" rel="nofollow">Request a Quote</a> <a href="/services/bulk-quote-request?catnum=DY8149-05&product=Human Ghrelin DuoSet ELISA" class="btn btn-secondary atc_hidden bulk_btn" style="display:none;" rel="nofollow" title="Competitive discounts on products purchased in bulk quantities">Bulk Orders</a> <button class="btn btn-primary atc_hidden atc_btn form-submit icon-before" style="display:none;" title="Enter quantities above and click to add to cart" type="submit" id="edit-add-to-cart" name="add_to_cart_btn" value="Add to Cart"><span class="icon glyphicon glyphicon-plus" aria-hidden="true"></span> Add to Cart</button> </div> <div class="ds_atc_post_buttons"></div> </div> <input type="hidden" name="form_build_id" value="form--vvZDUxeSR-Jp4Uh9DH5z5LNAfwPOgyKDOYIArKujp4" /> <input type="hidden" name="form_id" value="atc_datasheet_form" /> </div></form> </div> </div> <div class="product_image_wrapper col-xs-12 col-md-4 col-md-pull-8"> <div class="product_image"> <span class="product_image_label"> </span> <a class="ds-image overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="R&D Systems DuoSet ELISAs" data-footer="R&D Systems DuoSet ELISAs" title="Cat. No. DY8149-05 - Human Ghrelin DuoSet ELISA" href="https://resources.rndsystems.com/categoryimages/rndsystems_duoset-elisas.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/categoryimages/rndsystems_duoset-elisas.jpg" class="img-fluid" alt="R&D Systems DuoSet ELISAs" title="R&D Systems DuoSet ELISAs"> </a> <div class="search_image_other_count"> <span class="search_more_images">1 Image</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/ghrelin?category=" class="btn btn-secondary">All Ghrelin ELISAs</a> </div> </div> <div class="tabbed_content" id="rnd_elisabased"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_2" data-tab="2" data-tabname="assay-procedure" id="dsTabs_assay-procedure"> <span>Procedure</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (4)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_product_features" id="product-anchor-ds_product_features">Product Features</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_kit_content" id="product-anchor-ds_kit_content">Kit Content</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_other_reagents" id="product-anchor-ds_other_reagents">Other Reagents Required</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> </div> <div id="ds_summary" class="datasheet_section"> <h2>Human Ghrelin DuoSet ELISA Summary</h2> <div class="ds_table summary_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Type</div> <div class="ds_table_cell summary_table_data">Solid Phase Sandwich ELISA</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Format</div> <div class="ds_table_cell summary_table_data">96-well strip plate</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Range</div> <div class="ds_table_cell summary_table_data">31.3 - 2,000 pg/mL</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sufficient Materials</div> <div class="ds_table_cell summary_table_data">For five 96-well plates*</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data">Please see the <a href="//resources.rndsystems.com/pdfs/datasheets/dy8149.pdf" class="ds_pdf">product datasheet</a></div> </div> </div> </div> <p class="subtext">* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided. </p> <div id="ds_product_details" class="datasheet_section"> This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Ghrelin by itself or in context of the Ghrelin-Obstatin preprotein. The suggested diluent is suitable for the analysis of most cell culture supernate, tissue lysates, serum, and plasma samples. Diluents for complex matrices, such as serum, and plasma should be evaluated prior to use in this DuoSet.<br />   </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="DY8149-05"></div> <div id="ds_product_features" class="datasheet_section"> <h2>Product Features</h2> <ul> <li>Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time</li> <li>Development protocols are provided to guide further assay optimization</li> <li>Assay can be customized to your specific needs</li> <li>Economical alternative to complete kits</li> </ul> </div> <div id="ds_kit_content" class="datasheet_section"> <h2>Kit Content</h2> <ul> <li>Capture Antibody</li> <li>Detection Antibody</li> <li>Recombinant Standard</li> <li>Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)</li> </ul> </div> <div id="ds_other_reagents" class="datasheet_section"> <h2>Other Reagents Required</h2> <div class="other_reagent" id="reagents"><p><strong>DuoSet Ancillary Reagent Kit 2 (5 plates): </strong>(Catalog # <a href="/search?keywords=DY008">DY008</a>) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.</p> <p><strong>The components listed above may be purchased separately:</strong></p> <p><strong>PBS:</strong> (Catalog # <a href="/search?keywords=DY006">DY006</a>), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na<sub>2</sub>HPO<sub>4</sub>, 1.5 mM KH<sub>2</sub>PO<sub>4</sub>, pH 7.2 - 7.4, 0.2 µm filtered<br /> <br /> <strong>Wash Buffer:</strong> (Catalog # <a href="/search?keywords=WA126">WA126</a>), or 0.05% Tween<sup>®</sup> 20 in PBS, pH 7.2-7.4</p> <p><strong>Reagent Diluent: </strong>(Catalog # <a href="/search?keywords=DY995">DY995</a>), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered<br /> <br /> <strong>Substrate Solution:</strong> 1:1 mixture of Color Reagent A (H<sub>2</sub>O<sub>2</sub>) and Color Reagent B (Tetramethylbenzidine) (Catalog # <a href="/search?keywords=DY999">DY999</a>)<br /> <br /> <strong>Stop Solution:</strong> 2 N H<sub>2</sub>SO<sub>4</sub> (Catalog # <a href="/search?keywords=DY994">DY994</a>)</p> <p><strong>Microplates</strong>: R&D Systems (Catalog # <a href="/search?keywords=DY990">DY990</a>)<br /> <br /> <strong>Plate Sealers:</strong> ELISA Plate Sealers (Catalog # <a href="/search?keywords=DY992">DY992</a>)</p></div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/dy8149.pdf?v=20241123" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-ghrelin-duoset-elisa_dy8149-05">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//aero.bio-techne.com/sds/pdf/dy8149-05?v=20241123">SDS</a> </div> </div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Stability & Storage</div> <div class="ds_table_cell prep_table_data">Store the unopened product at 2 - 8 °C. Do not use past expiration date. </div> </div> </div> </div> </div> </div> <div class="tab_content tab_2" data-tab="2" data-tabname="product-assay-procedure" id="product-assay-procedure"> <a id="assay-procedure"></a> <div id="boxes">  <h2>Assay Procedure</h2> <h3><strong>GENERAL ELISA PROTOCOL</strong></h3> <h4><strong>Plate Preparation</strong></h4> <ol> <li>Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.</li> <li>Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.</li> <li>Block plates by adding 300 μL of Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.</li> <li>Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.</li> </ol> <h4><strong>Assay Procedure</strong></h4> <ol> <li>Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.</li> <li>Repeat the aspiration/wash as in step 2 of Plate Preparation.</li> <li>Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.</li> <li>Repeat the aspiration/wash as in step 2 of Plate Preparation.</li> <li>Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.</li> <li>Repeat the aspiration/wash as in step 2.</li> <li>Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.</li> <li>Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.</li> <li>Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.</li> </ol> </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for Human Ghrelin DuoSet ELISA</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">4</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">4</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Human"> Human </option> <option class="citation_filterRadio" value="species" data-filter="Mouse"> Mouse </option> <option class="citation_filterRadio" value="species" data-filter="Transgenic Mouse"> Transgenic Mouse </option> </select> </div> <input type="hidden" id="selAntibody" value="All"> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="Serum"> Serum </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="4" data-max-show="10"> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/36596839"> Assessment of metabolic syndrome predictors in relation to inflammation and visceral fat tissue in older adults </a> </span><br/> Authors: A Tylutka, B Morawin, ? Walas, M Micha?ek, A Gwara, A Zembron-La<br/> Scientific Reports, 2023-01-03;13(1):89.<br/> Species: Human<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/34829886"> New Insights on the Relationship between Leptin, Ghrelin, and Leptin/Ghrelin Ratio Enforced by Body Mass Index in Obesity and Diabetes </a> </span><br/> Authors: AV Sitar-T?ut, A Cozma, A Fodor, SC Coste, OH Orasan, V Negrean, D Pop, DA Sitar-T?ut<br/> Biomedicines, 2021-11-10;9(11):.<br/> Species: Human<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/34226737"> Fecal microbial transplantation and fiber supplementation in patients with severe obesity and metabolic syndrome: a randomized double-blind, placebo-controlled phase 2 trial </a> </span><br/> Authors: V Mocanu, Z Zhang, EC Deehan, DH Kao, N Hotte, S Karmali, DW Birch, KK Samarasing, J Walter, KL Madsen<br/> Nature Medicine, 2021-07-05;27(7):1272-1279.<br/> Species: Human<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse, Transgenic Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/32585823"> Interleukin-4 Improves Metabolic Abnormalities in Leptin-Deficient and High-Fat Diet Mice </a> </span><br/> Authors: SY Lin, CP Yang, YY Wang, CW Hsiao, WY Chen, SL Liao, YL Lo, YH Chang, CJ Hong, CJ Chen<br/> Int J Mol Sci, 2020-06-23;21(12):.<br/> Species: Mouse, Transgenic Mouse<br/> Sample Types: Serum<br/> </li> </ol> </div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/elisas"> View all ELISA FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="dy8149-05" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Human Ghrelin DuoSet ELISA</h3> <div class="row"> <div class="col-xs-12"> <div> <div class="row"> <div class="col-md-6"> <p>There are currently no reviews for this product. 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