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multi-function</title> <meta name="description" content="Search results for: multi-function"> <meta name="keywords" content="multi-function"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="multi-function"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 8</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: multi-function</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> A Detailed Study of Two Different Airfoils on Flight Performance of MAV of Same Physical Dimension </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shoeb%20A.%20Adeel">Shoeb A. Adeel</a>, <a href="https://publications.waset.org/abstracts/search?q=Shashant%20Anand"> Shashant Anand</a>, <a href="https://publications.waset.org/abstracts/search?q=Vivek%20Paul"> Vivek Paul</a>, <a href="https://publications.waset.org/abstracts/search?q=Dinesh"> Dinesh</a>, <a href="https://publications.waset.org/abstracts/search?q=Suraj"> Suraj</a>, <a href="https://publications.waset.org/abstracts/search?q=Roshan"> Roshan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The paper presents a study of micro air vehicles (MAVs) with wingspans of 20 Cm with two different airfoil configurations. MAVs have vast potential applications in both military and civilian areas. These MAVs are fully autonomous and supply real-time data. The paper focuses on two different designs of the MAVs one being N22 airfoil and the other a flat plate with similar dimension. As designed, the MAV would fly in a low Reynolds-number regime at airspeeds of 15 & 20 m/sec. Propulsion would be provided by an electric motor with an advanced lithium. Because of the close coupling between vehicle elements, system integration would be a significant challenge, requiring tight packaging and multifunction components to meet mass limitations and Centre of Gravity (C.G) balancing. These MAVs are feasible and within a couple of years of technology development in key areas including sensors, propulsion, Aerodynamics, and packaging these would be easily available to the users at affordable prices. The paper finally compares the flight performance of the two configurations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=airfoil" title="airfoil">airfoil</a>, <a href="https://publications.waset.org/abstracts/search?q=CFD" title=" CFD"> CFD</a>, <a href="https://publications.waset.org/abstracts/search?q=MAV" title=" MAV"> MAV</a>, <a href="https://publications.waset.org/abstracts/search?q=flight%20performance" title=" flight performance"> flight performance</a>, <a href="https://publications.waset.org/abstracts/search?q=endurance" title=" endurance"> endurance</a>, <a href="https://publications.waset.org/abstracts/search?q=climb" title=" climb"> climb</a>, <a href="https://publications.waset.org/abstracts/search?q=lift" title=" lift"> lift</a>, <a href="https://publications.waset.org/abstracts/search?q=drag" title=" drag"> drag</a> </p> <a href="https://publications.waset.org/abstracts/35290/a-detailed-study-of-two-different-airfoils-on-flight-performance-of-mav-of-same-physical-dimension" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35290.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">496</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Ecological Implication of Air Pollution From Quarrying and Stone Cutting Industries on Agriculture and Plant Biodiversity Around Quarry Sites in Mpape, Bwari Area Council, FCT, Abuja</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammed%20Rabiu">Muhammed Rabiu</a>, <a href="https://publications.waset.org/abstracts/search?q=Moses%20S.%20Oluyomi"> Moses S. Oluyomi</a>, <a href="https://publications.waset.org/abstracts/search?q=Joshua%20Olorundare"> Joshua Olorundare</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Quarry activities are important to modern day life and the socio-economic development of local communities. Unfortunately, this industry is usually associated with air pollution. To assess the impact of quarry dust on plant biodiversity and agriculture, PM2.5, PM10 and some meteorological parameters were measured using Gas analyzer, handheld thermometer and Multifunction Anemometer (PCE-EM 888) as well as taking a social survey. High amount of particulate matters that exceeded the international standard were recorded at the study locations which include the Julius Berger Quarry and 1km away from the quarry site which serve as the base for the farmlands. The correlation coefficient between the particulate matters with the meteorological parameters of the locations all show a strong relationship with temperature recording a stronger value of 0.952 and 0.931 for PM2.5 and PM10 respectively. Similarly, the coefficient of determination 0.906 and 0.866 shows that temperature has the highest meteorological percentage variation on PM2.5 and PM10. Furthermore, a notable negative impact of quarrying on plant biodiversity and local farm crops are also revealed based on respondents’ results where wide range of local plants were affected with Maize and Azadiracta indica (Neem) been the most with respondent of 31.5% and 27.5%. According to the obtained results, it is highly recommended to develop green belt surrounding the quarrying using pollutant-tolerant trees (usually with broad leaves) in order to restrict spreading of quarrying dust via intercepting, filtering and absorbing pollutants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=agriculture" title="agriculture">agriculture</a>, <a href="https://publications.waset.org/abstracts/search?q=air%20pollution" title=" air pollution"> air pollution</a>, <a href="https://publications.waset.org/abstracts/search?q=biodiversity" title=" biodiversity"> biodiversity</a>, <a href="https://publications.waset.org/abstracts/search?q=quarry" title=" quarry"> quarry</a> </p> <a href="https://publications.waset.org/abstracts/162505/ecological-implication-of-air-pollution-from-quarrying-and-stone-cutting-industries-on-agriculture-and-plant-biodiversity-around-quarry-sites-in-mpape-bwari-area-council-fct-abuja" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162505.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">83</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Spatial Integration at the Room-Level of 'Sequina' Slum Area in Alexandria, Egypt</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ali%20Essam%20El%20Shazly">Ali Essam El Shazly</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The slum survey of 'Sequina' area in Alexandria details the building rooms of twenty-building samples according to the integral measure of space syntax. The essence of room organization sets the most integrative 'visitor' domain between the 'inhabitant' wings of less integrated 'parent' than the 'children' structure with visual ring of 'balcony' space. Despite the collective real relative asymmetry of 'pheno-type' aggregation, the relative asymmetry of individual layouts reveals 'geno-type' structure of spatial diversity. The multifunction of rooms optimizes the integral structure of graph and visibility merge, which contrasts with the deep tailing structure of distinctive social domains. The most integrative layout inverts the geno-type into freed rooms of shallow 'inhabitant' domain against the off-centered 'visitor' space, while the most segregated layout further restricts the pheno-type through isolated 'visitor' from 'inhabitant' domains across the 'staircase' public domain. The catalyst 'kitchen & living' spaces demonstrate multi-structural dimensions among the various social domains. The former ranges from most exposed central integrity to the most hidden 'motherhood' territories. The latter, however, mostly integrates at centrality or at the further ringy 'childern' domain. The study concludes social structure of spatial integrity for redevelopment, which is determined through the micro-level survey of rooms with integral dimensions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alexandria" title="Alexandria">Alexandria</a>, <a href="https://publications.waset.org/abstracts/search?q=Sequina%20slum" title=" Sequina slum"> Sequina slum</a>, <a href="https://publications.waset.org/abstracts/search?q=spatial%20integration" title=" spatial integration"> spatial integration</a>, <a href="https://publications.waset.org/abstracts/search?q=space%20syntax" title=" space syntax"> space syntax</a> </p> <a href="https://publications.waset.org/abstracts/41527/spatial-integration-at-the-room-level-of-sequina-slum-area-in-alexandria-egypt" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41527.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">438</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> 150 KVA Multifunction Laboratory Test Unit Based on Power-Frequency Converter</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bartosz%20Kedra">Bartosz Kedra</a>, <a href="https://publications.waset.org/abstracts/search?q=Robert%20Malkowski"> Robert Malkowski</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper provides description and presentation of laboratory test unit built basing on 150 kVA power frequency converter and Simulink RealTime platform. Assumptions, based on criteria which load and generator types may be simulated using discussed device, are presented, as well as control algorithm structure. As laboratory setup contains transformer with thyristor controlled tap changer, a wider scope of setup capabilities is presented. Information about used communication interface, data maintenance, and storage solution as well as used Simulink real-time features is presented. List and description of all measurements are provided. Potential of laboratory setup modifications is evaluated. For purposes of Rapid Control Prototyping, a dedicated environment was used Simulink RealTime. Therefore, load model Functional Unit Controller is based on a PC computer with I/O cards and Simulink RealTime software. Simulink RealTime was used to create real-time applications directly from Simulink models. In the next step, applications were loaded on a target computer connected to physical devices that provided opportunity to perform Hardware in the Loop (HIL) tests, as well as the mentioned Rapid Control Prototyping process. With Simulink RealTime, Simulink models were extended with I/O cards driver blocks that made automatic generation of real-time applications and performing interactive or automated runs on a dedicated target computer equipped with a real-time kernel, multicore CPU, and I/O cards possible. Results of performed laboratory tests are presented. Different load configurations are described and experimental results are presented. This includes simulation of under frequency load shedding, frequency and voltage dependent characteristics of groups of load units, time characteristics of group of different load units in a chosen area and arbitrary active and reactive power regulation basing on defined schedule. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=MATLAB" title="MATLAB">MATLAB</a>, <a href="https://publications.waset.org/abstracts/search?q=power%20converter" title=" power converter"> power converter</a>, <a href="https://publications.waset.org/abstracts/search?q=Simulink%20Real-Time" title=" Simulink Real-Time"> Simulink Real-Time</a>, <a href="https://publications.waset.org/abstracts/search?q=thyristor-controlled%20tap%20changer" title=" thyristor-controlled tap changer"> thyristor-controlled tap changer</a> </p> <a href="https://publications.waset.org/abstracts/50924/150-kva-multifunction-laboratory-test-unit-based-on-power-frequency-converter" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/50924.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">323</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> A Comparative Analysis of Innovation Maturity Models: Towards the Development of a Technology Management Maturity Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nikolett%20Deutsch">Nikolett Deutsch</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%89va%20Pint%C3%A9r"> Éva Pintér</a>, <a href="https://publications.waset.org/abstracts/search?q=P%C3%A9ter%20Bag%C3%B3"> Péter Bagó</a>, <a href="https://publications.waset.org/abstracts/search?q=Mikl%C3%B3s%20Het%C3%A9nyi"> Miklós Hetényi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Strategic technology management has emerged and evolved parallelly with strategic management paradigms. It focuses on the opportunity for organizations operating mainly in technology-intensive industries to explore and exploit technological capabilities upon which competitive advantage can be obtained. As strategic technology management involves multifunction within an organization, requires broad and diversified knowledge, and must be developed and implemented with business objectives to enable a firm’s profitability and growth, excellence in strategic technology management provides unique opportunities for organizations in terms of building a successful future. Accordingly, a framework supporting the evaluation of the technological readiness level of management can significantly contribute to developing organizational competitiveness through a better understanding of strategic-level capabilities and deficiencies in operations. In the last decade, several innovation maturity assessment models have appeared and become designated management tools that can serve as references for future practical approaches expected to be used by corporate leaders, strategists, and technology managers to understand and manage technological capabilities and capacities. The aim of this paper is to provide a comprehensive review of the state-of-the-art innovation maturity frameworks, to investigate the critical lessons learned from their application, to identify the similarities and differences among the models, and identify the main aspects and elements valid for the field and critical functions of technology management. To this end, a systematic literature review was carried out considering the relevant papers and articles published in highly ranked international journals around the 27 most widely known innovation maturity models from four relevant digital sources. Key findings suggest that despite the diversity of the given models, there is still room for improvement regarding the common understanding of innovation typologies, the full coverage of innovation capabilities, and the generalist approach to the validation and practical applicability of the structure and content of the models. Furthermore, the paper proposes an initial structure by considering the maturity assessment of the technological capacities and capabilities - i.e., technology identification, technology selection, technology acquisition, technology exploitation, and technology protection - covered by strategic technology management. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=innovation%20capabilities" title="innovation capabilities">innovation capabilities</a>, <a href="https://publications.waset.org/abstracts/search?q=innovation%20maturity%20models" title=" innovation maturity models"> innovation maturity models</a>, <a href="https://publications.waset.org/abstracts/search?q=technology%20audit" title=" technology audit"> technology audit</a>, <a href="https://publications.waset.org/abstracts/search?q=technology%20management" title=" technology management"> technology management</a>, <a href="https://publications.waset.org/abstracts/search?q=technology%20management%20maturity%20models" title=" technology management maturity models"> technology management maturity models</a> </p> <a href="https://publications.waset.org/abstracts/182990/a-comparative-analysis-of-innovation-maturity-models-towards-the-development-of-a-technology-management-maturity-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/182990.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">61</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Characterization of Kevlar 29 for Multifunction Applications</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Doaa%20H.%20Elgohary">Doaa H. Elgohary</a>, <a href="https://publications.waset.org/abstracts/search?q=Dina%20M.%20Hamoda"> Dina M. Hamoda</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Yahia"> S. Yahia</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Technical textiles refer to textile materials that are engineered and designed to have specific functionalities and performance characteristics beyond their traditional use as apparel or upholstery fabrics. These textiles are usually developed for their unique properties such as strength, durability, flame retardancy, chemical resistance, waterproofing, insulation and other special properties. The development and use of technical textiles are constantly evolving, driven by advances in materials science, manufacturing technologies and the demand for innovative solutions in various industries. Kevlar 29 is a type of aramid fiber developed by DuPont. It is a high-performance material known for its exceptional strength and resistance to impact, abrasion, and heat. Kevlar 29 belongs to the Kevlar family, which includes different types of aramid fibers. Kevlar 29 is primarily used in applications that require strength and durability, such as ballistic protection, body armor, and body armor for military and law enforcement personnel. It is also used in the aerospace and automotive industries to reinforce composite materials, as well as in various industrial applications. Two different Kevlar samples were used coated with cooper lithium silicate (CLS); ten different mechanical and physical properties (weight, thickness, tensile strength, elongation, stiffness, air permeability, puncture resistance, thermal conductivity, stiffness, and spray test) were conducted to approve its functional performance efficiency. The influence of different mechanical properties was statistically analyzed using an independent t-test with a significant difference at P-value = 0.05. The radar plot was calculated and evaluated to determine the best-performing samples. The results of the independent t-test observed that all variables were significantly affected by yarn counts except water permeability, which has no significant effect. All properties were evaluated for samples 1 and 2, a radar chart was used to determine the best attitude for samples. The radar chart area was calculated, which shows that sample 1 recorded the best performance, followed by sample 2. The surface morphology of all samples and the coating materials was determined using a scanning electron microscope (SEM), also Fourier Transform Infrared Spectroscopy Measurement for the two samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cooper%20lithium%20silicate" title="cooper lithium silicate">cooper lithium silicate</a>, <a href="https://publications.waset.org/abstracts/search?q=independent%20t-test" title=" independent t-test"> independent t-test</a>, <a href="https://publications.waset.org/abstracts/search?q=kevlar" title=" kevlar"> kevlar</a>, <a href="https://publications.waset.org/abstracts/search?q=technical%20textiles." title=" technical textiles."> technical textiles.</a> </p> <a href="https://publications.waset.org/abstracts/169696/characterization-of-kevlar-29-for-multifunction-applications" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/169696.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">80</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Microstructure and Mechanical Properties Evaluation of Graphene-Reinforced AlSi10Mg Matrix Composite Produced by Powder Bed Fusion Process</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jitendar%20Kumar%20Tiwari">Jitendar Kumar Tiwari</a>, <a href="https://publications.waset.org/abstracts/search?q=Ajay%20Mandal"> Ajay Mandal</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Sathish"> N. Sathish</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20K.%20Srivastava"> A. K. Srivastava</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Since the last decade, graphene achieved great attention toward the progress of multifunction metal matrix composites, which are highly demanded in industries to develop energy-efficient systems. This study covers the two advanced aspects of the latest scientific endeavor, i.e., graphene as reinforcement in metallic materials and additive manufacturing (AM) as a processing technology. Herein, high-quality graphene and AlSi10Mg powder mechanically mixed by very low energy ball milling with 0.1 wt. % and 0.2 wt. % graphene. Mixed powder directly subjected to the powder bed fusion process, i.e., an AM technique to produce composite samples along with bare counterpart. The effects of graphene on porosity, microstructure, and mechanical properties were examined in this study. The volumetric distribution of pores was observed under X-ray computed tomography (CT). On the basis of relative density measurement by X-ray CT, it was observed that porosity increases after graphene addition, and pore morphology also transformed from spherical pores to enlarged flaky pores due to improper melting of composite powder. Furthermore, the microstructure suggests the grain refinement after graphene addition. The columnar grains were able to cross the melt pool boundaries in case of the bare sample, unlike composite samples. The smaller columnar grains were formed in composites due to heterogeneous nucleation by graphene platelets during solidification. The tensile properties get affected due to induced porosity irrespective of graphene reinforcement. The optimized tensile properties were achieved at 0.1 wt. % graphene. The increment in yield strength and ultimate tensile strength was 22% and 10%, respectively, for 0.1 wt. % graphene reinforced sample in comparison to bare counterpart while elongation decreases 20% for the same sample. The hardness indentations were taken mostly on the solid region in order to avoid the collapse of the pores. The hardness of the composite was increased progressively with graphene content. Around 30% of increment in hardness was achieved after the addition of 0.2 wt. % graphene. Therefore, it can be concluded that powder bed fusion can be adopted as a suitable technique to develop graphene reinforced AlSi10Mg composite. Though, some further process modification required to avoid the induced porosity after the addition of graphene, which can be addressed in future work. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=graphene" title="graphene">graphene</a>, <a href="https://publications.waset.org/abstracts/search?q=hardness" title=" hardness"> hardness</a>, <a href="https://publications.waset.org/abstracts/search?q=porosity" title=" porosity"> porosity</a>, <a href="https://publications.waset.org/abstracts/search?q=powder%20bed%20fusion" title=" powder bed fusion"> powder bed fusion</a>, <a href="https://publications.waset.org/abstracts/search?q=tensile%20properties" title=" tensile properties"> tensile properties</a> </p> <a href="https://publications.waset.org/abstracts/118429/microstructure-and-mechanical-properties-evaluation-of-graphene-reinforced-alsi10mg-matrix-composite-produced-by-powder-bed-fusion-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/118429.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">127</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Biophysical and Structural Characterization of Transcription Factor Rv0047c of Mycobacterium Tuberculosis H37Rv</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Md.%20Samsuddin%20Ansari">Md. Samsuddin Ansari</a>, <a href="https://publications.waset.org/abstracts/search?q=Ashish%20Arora"> Ashish Arora</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Every year 10 million people fall ill with one of the oldest diseases known as tuberculosis, caused by Mycobacterium tuberculosis. The success of M. tuberculosis as a pathogen is because of its ability to persist in host tissues. Multidrug resistance (MDR) mycobacteria cases increase every day, which is associated with efflux pumps controlled at the level of transcription. The transcription regulators of MDR transporters in bacteria belong to one of the following four regulatory protein families: AraC, MarR, MerR, and TetR. Phenolic acid decarboxylase repressor (PadR), like a family of transcription regulators, is closely related to the MarR family. Phenolic acid decarboxylase repressor (PadR) was first identified as a transcription factor involved in the regulation of phenolic acid stress response in various microorganisms (including Mycobacterium tuberculosis H37Rv). Recently research has shown that the PadR family transcription factors are global, multifunction transcription regulators. Rv0047c is a PadR subfamily-1 protein. We are exploring the biophysical and structural characterization of Rv0047c. The Rv0047 gene was amplified by PCR using the primers containing EcoRI and HindIII restriction enzyme sites cloned in pET-NH6 vector and overexpressed in DH5α and BL21 (λDE3) cells of E. coli following purification with Ni2+-NTA column and size exclusion chromatography. We did DSC to know the thermal stability; the Tm (transition temperature) of protein is 55.29ºC, and ΔH (enthalpy change) of 6.92 kcal/mol. Circular dichroism to know the secondary structure and conformation and fluorescence spectroscopy for tertiary structure study of protein. To understand the effect of pH on the structure, function, and stability of Rv0047c we employed spectroscopy techniques such as circular dichroism, fluorescence, and absorbance measurements in a wide range of pH (from pH-2.0 to pH-12). At low and high pH, it shows drastic changes in the secondary and tertiary structure of the protein. EMSA studies showed the specific binding of Rv0047c with its own 30-bp promoter region. To determine the effect of complex formation on the secondary structure of Rv0047c, we examined the CD spectra of the complex of Rv0047c with promoter DNA of rv0047. The functional role of Rv0047c was characterized by over-expressing the Rv0047c gene under the control of hsp60 promoter in Mycobacterium tuberculosis H37Rv. We have predicted the three-dimensional structure of Rv0047c using the Swiss Model and Modeller, with validity checked by the Ramachandra plot. We did molecular docking of Rv0047c with dnaA, through PatchDock following refinement through FireDock. Through this, it is possible to easily identify the binding hot-stop of the receptor molecule with that of the ligand, the nature of the interface itself, and the conformational change undergone by the protein pattern. We are using X-crystallography to unravel the structure of Rv0047c. Overall the studies show that Rv0047c may have transcription regulation along with providing an insight into the activity of Rv0047c in the pH range of subcellular environment and helps to understand the protein-protein interaction, a novel target to kill dormant bacteria and potential strategy for tuberculosis control. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mycobacterium%20tuberculosis" title="mycobacterium tuberculosis">mycobacterium tuberculosis</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolic%20acid%20decarboxylase%20repressor" title=" phenolic acid decarboxylase repressor"> phenolic acid decarboxylase repressor</a>, <a href="https://publications.waset.org/abstracts/search?q=Rv0047c" title=" Rv0047c"> Rv0047c</a>, <a href="https://publications.waset.org/abstracts/search?q=Circular%20dichroism" title=" Circular dichroism"> Circular dichroism</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescence%20spectroscopy" title=" fluorescence spectroscopy"> fluorescence spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=docking" title=" docking"> docking</a>, <a href="https://publications.waset.org/abstracts/search?q=protein-protein%20interaction" title=" protein-protein interaction"> protein-protein interaction</a> </p> <a href="https://publications.waset.org/abstracts/161001/biophysical-and-structural-characterization-of-transcription-factor-rv0047c-of-mycobacterium-tuberculosis-h37rv" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/161001.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">121</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div 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