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Search results for: photodamage
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photodamage</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Chromosomal Damage in Human Lymphocytes by Ultraviolet Radiation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Felipe%20Osorio%20Ospina">Felipe Osorio Ospina</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Adelaida%20Mejia%20Arango"> Maria Adelaida Mejia Arango</a>, <a href="https://publications.waset.org/abstracts/search?q=Esteban%20On%C3%A9simo%20Vallejo%20Agudelo"> Esteban On茅simo Vallejo Agudelo</a>, <a href="https://publications.waset.org/abstracts/search?q=Victoria%20Luc%C3%ADa%20D%C3%A1vila%20Osorio"> Victoria Luc铆a D谩vila Osorio</a>, <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Vargas%20Grisales"> Natalia Vargas Grisales</a>, <a href="https://publications.waset.org/abstracts/search?q=Lina%20Mar%C3%ADa%20Mart%C3%ADnez%20Sanchez"> Lina Mar铆a Mart铆nez Sanchez</a>, <a href="https://publications.waset.org/abstracts/search?q=Camilo%20Andr%C3%A9s%20Agudelo%20V%C3%A9lez"> Camilo Andr茅s Agudelo V茅lez</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%81ngela%20Maria%20Londo%C3%B1o%20Garc%C3%ADa"> 脕ngela Maria Londo帽o Garc铆a</a>, <a href="https://publications.waset.org/abstracts/search?q=Isabel%20Cristina%20Ortiz%20Trujillo"> Isabel Cristina Ortiz Trujillo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Excessive exposure to ultraviolet radiation, has shown to be a risk factor for photodamage, alteration of the immune mechanisms to recognize malignant cells and cutaneous pro-inflamatorios States and skin cancers. Objective: Identify the time of exposure to ultraviolet radiation for the production of chromosomal damage in human lymphocytes. Methodology: We conducted an in vitro study serial, in which samples were taken from heparinized blood of healthy people, who do not submit exposure to agents that could induce chromosomal alterations. The samples were cultured in RPMI-1640 medium containing 10% fetal bovine serum, penicillin and streptomycin antibiotic. Subsequently, they were grouped and exposed to ultraviolet light for 1 to 20 seconds. At the end of the treatments, cytology samples were prepared, and it was colored with Giemsa (5%). Reading was carried out in an optical microscope and 100 metaphases analysed by treatment for posting chromosomal alterations. Each treatment was conducted at three separate times and each became two replicas. Results: We only presented chromosomal alterations in lymphocytes exposed to UV for a groups 1 to 3 seconds (p<0.05). Conclusions: Exposure to ultraviolet radiation generates visible damage in chromosomes from human lymphocytes observed in light microscopy, the highest rates of injury was observed between two and three seconds, and above this value, the reduction in the number of mitotic cells was evident. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ultraviolet%20rays" title="ultraviolet rays">ultraviolet rays</a>, <a href="https://publications.waset.org/abstracts/search?q=lymphocytes" title=" lymphocytes"> lymphocytes</a>, <a href="https://publications.waset.org/abstracts/search?q=chromosome%20breakpoints" title=" chromosome breakpoints"> chromosome breakpoints</a>, <a href="https://publications.waset.org/abstracts/search?q=photodamage" title=" photodamage"> photodamage</a> </p> <a href="https://publications.waset.org/abstracts/14162/chromosomal-damage-in-human-lymphocytes-by-ultraviolet-radiation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/14162.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">428</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Ultraviolet Radiation and Chromosomal Damage in Human Lymphocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Felipe%20Osorio%20Ospina">Felipe Osorio Ospina</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Adelaida%20Mejia%20Arango"> Maria Adelaida Mejia Arango</a>, <a href="https://publications.waset.org/abstracts/search?q=Esteban%20On%C3%A9simo%20Vallejo%20Agudelo"> Esteban On茅simo Vallejo Agudelo</a>, <a href="https://publications.waset.org/abstracts/search?q=Victoria%20Luc%C3%ADa%20D%C3%A1vila%20Osorio"> Victoria Luc铆a D谩vila Osorio</a>, <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Vargas%20Grisales"> Natalia Vargas Grisales</a>, <a href="https://publications.waset.org/abstracts/search?q=Lina%20Mar%C3%ADa%20Mart%C3%ADnez%20Sanchez"> Lina Mar铆a Mart铆nez Sanchez</a>, <a href="https://publications.waset.org/abstracts/search?q=Camilo%20Andr%C3%A9s%20Agudelo%20V%C3%A9lez"> Camilo Andr茅s Agudelo V茅lez</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%81ngela%20Maria%20Londo%C3%B1o%20Garc%C3%ADa"> 脕ngela Maria Londo帽o Garc铆a</a>, <a href="https://publications.waset.org/abstracts/search?q=Isabel%20Cristina%20Ortiz%20Trujillo"> Isabel Cristina Ortiz Trujillo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Excessive exposure to ultraviolet radiation, has shown to be a risk factor for photodamage, alteration of the immune mechanisms to recognize malignant cells and cutaneous pro-inflamatorios states and skin cancers. Objective: To identify the time of exposure to ultraviolet radiation for the production of chromosomal damage in human lymphocytes. Methodology: We conducted an in vitro study serial, in which samples were taken from the heparinized blood of healthy people, who do not submit exposure to agents that could induce chromosomal alterations. The samples were cultured in RPMI-1640 medium containing 10% fetal bovine serum, penicillin, and streptomycin antibiotic. Subsequently, they were grouped and exposed to ultraviolet light for 1 to 20 seconds. At the end of the treatments, cytology samples were prepared, and it was colored with Giemsa (5%). Reading was carried out in an optical microscope and 100 metaphases analysed by treatment for posting chromosomal alterations. Each treatment was conducted at three separate times and each became two replicas. Results: We only presented chromosomal alterations in lymphocytes exposed to UV for groups 1 to 3 seconds (p < 0.05). Conclusions: Exposure to ultraviolet radiation generates visible damage in chromosomes from human lymphocytes observed in light microscopy, the highest rates of injury was observed between two and three seconds, and above this value, the reduction in the number of mitotic cells was evident. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chromosome%20breakpoints" title="chromosome breakpoints">chromosome breakpoints</a>, <a href="https://publications.waset.org/abstracts/search?q=lymphocytes" title=" lymphocytes"> lymphocytes</a>, <a href="https://publications.waset.org/abstracts/search?q=photodamage" title=" photodamage"> photodamage</a>, <a href="https://publications.waset.org/abstracts/search?q=ultraviolet%20rays" title=" ultraviolet rays"> ultraviolet rays</a> </p> <a href="https://publications.waset.org/abstracts/26760/ultraviolet-radiation-and-chromosomal-damage-in-human-lymphocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26760.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">578</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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