RNAscope™ Chromogenic Assays

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</div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#features">Product Features</a> | <a href="#options">Technology Options</a> | <a href="#resources">Resources</a> </p><p> </p><p>Advanced Cell Diagnostics, a Bio-Techne brand offers multiple, unique options for RNA chromogenic in situ hybridization assays (RNA CISH). Two types of detection chemistries are available depending on your research need, preferred manual or automated workflow, and your preferred imaging platform. </p></div> </div> </div> <div> <div class="paragraph paragraph--type--individual-button mb-4 paragraph--view-mode--default w-100"> <div class="row"> <div class="col"> <a href="/reagents/rnascope-ish-technology" class="btn btn-primary" type="link" > Learn More About RNAscope Technology </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="features"></a>Product Line Features </h2></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/rnascope-chromogenic-sensitivity-specificity-cervical-cancer.jpg" alt="RNAscope chromogenic assay image of cervical cancer tissue" loading="lazy" > </div> <h3 class="card_title_htag"> <div><h3>Unprecedented Sensitivity and Specificity </h3></div> </h3> <div class="card_description flex-grow-1"> <div><ul><li lang="EN-US">RNAscope assays allow single RNA molecule detection with our ACD patented Z probe pair design</li><li lang="EN-US">Rigorous double Z probes design eliminates cross-hybridization to unintended targets and routinely distinguishes RNA sequences with up to 85% homology</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/rnascope-chromogenic-universal.jpg" alt="RNAscope chromogenic assay image" loading="lazy" > </div> <h3 class="card_title_htag"> <div><h3>Universal </h3></div> </h3> <div class="card_description flex-grow-1"> <div><ul><li>Our RNAscope ISH assays work for virtually ANY gene in ANY species in ANY tissue</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/rnascope-chromogenic-duplex-assay-colon-cancer-v2.jpg" alt="RNAscope chromogenic assay image in colon cancer tissue" loading="lazy" > </div> <h3 class="card_title_htag"> <div><h3>Flexible Workflows </h3></div> </h3> <div class="card_description flex-grow-1"> <div><ul><li lang="EN-US">Assay options for manual use or increasing throughput of sample analysis using automated platforms or manual use</li><li lang="EN-US">Two chemistry options to fit needs</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="options"></a>Technology Options</h2></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/rnascope-single-plex-chromogenic-assay.jpg" alt="RNAscope single-plex chromogenic assay image showing Wnt4 in eye tissue" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>RNAscope Single-plex Chromogenic Assays<h3></h3></h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><ul><li><strong>RNAscope 2.5 HD Manual </strong>assay enables single target detection with bright field microscopy using either <a href="https://www.bio-techne.com/p/in-situ-hybridization-assays/rnascope-25-hd-detection-reagents-brown_322310">Diaminobenzidine (DAB)</a> or <a href="https://www.bio-techne.com/p/in-situ-hybridization-assays/rnascope-25-hd-detection-reagents-red_322360">Fast Red</a> dyes</li><li><a href="https://www.bio-techne.com/p/in-situ-hybridization-assays/rnascope-vs-universal-hrp-reagent-kit_323200"><strong>RNAscope VS Universal HRP</strong></a><strong> </strong>and<strong> </strong><a href="https://www.bio-techne.com/p/in-situ-hybridization-assays/rnascope-vs-universal-ap-reagent-kit_323250"><strong>RNAscope VS Universal AP</strong></a><strong> </strong>assays are our automated solutions enabling customers with high throughput single RNA detection on the <a href="/reagents/rnascope-ish-technology/roche-automation-assays">Roche DISCOVERY ULTRA</a></li><li><a href="/p/in-situ-hybridization-assays/rnascope-25-ls-reagent-kit-brown_322100"><strong>RNAscope 2.5 LS Brown</strong></a><strong>, </strong><a href="/p/in-situ-hybridization-assays/rnascope-25-ls-reagent-kit-red_322150"><strong>RNAscope 2.5 LS Red</strong></a><strong>, </strong><a href="/p/in-situ-hybridization-assays/rnascope-25-lsx-reagent-kit-brown_322700"><strong>RNAscope 2.5 LSx Brown</strong></a><strong> </strong>or<strong> </strong><a href="/p/in-situ-hybridization-assays/rnascope-25-lsx-reagent-kit-red_322750"><strong>RNAscope 2.5 LSx Red</strong></a> assays are our automated solution which enables customers with high throughput detection of RNA targets using the <a href="/reagents/rnascope-ish-technology/leica-automation-assays">Leica BOND RX</a> research staining platform</li></ul></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/rnascope-duplex-chromogenic-assay.jpg" alt="RNAscope duplex chromogenic assay in colon cancer tissue" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>RNAscope Duplex Chromogenic Assays</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Our RNAscope Duplex assays are designed for simultaneous <em>in situ </em>detection of two RNA species using probe channels C1 and C2. Common applications include co-localization studies to map co-expression of two targets within the same cellular context (e.g. secreted ligand and its receptor) or to profile gene expression in a specific cell type expressing a known marker (e.g. a specific <a href="/research-areas/stem-cells/stem-cell-markers">stem cell marker</a>). </p><ul><li><a href="/p/in-situ-hybridization-assays/rnascope-25-hd-duplex-reagent-kit_322430"><strong>RNAscope™ 2.5 HD Duplex Assay</strong></a><strong> – </strong>enables manual detection of two RNA targets using HRP-based Green and AP-based Fast Red chromogens </li><li><a href="/p/in-situ-hybridization-assays/rnascope-vs-duplex-reagent-kit_323300"><strong>RNAscope™ VS Duplex Assay</strong></a><strong> – </strong>enables high throughput detection of two RNA targets on the</li><li><a href="/p/in-situ-hybridization-assays/rnascope-25-ls-duplex-reagent-kit-redbrown_322440"><strong>RNAscope™ 2.5 LS Duplex Assay</strong></a> offers 12-plex RNA detection in FFPE tissues and up to 48-plex RNA detection in frozen tissues with use of HiPlex Up.</li></ul></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="resources"></a>Resources</h2></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/rnascope-manual-solutions-brochure" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/pdfs/maunal_solutions_brochure.jpg" alt="An image of the cover of the manual solutions brochure" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/rnascope-manual-solutions-brochure" > <h3 class="card_title_htag"> <div><h3>RNAscope Manual Solutions Brochure</h3></div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/rnascope-manual-solutions-brochure" > Download Brochure </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/webinars/from-small-rnas-to-chromosomes-an-overview-of-rnascope-technology-and-spatial-biology-tools" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/RNAscope-chromogenic-assay-ls25-red-head-neck-cancer-hpv16.jpg" alt="RNAscope™ 2.5 LS Reagent-RED: FFPE tissue, human head and neck cancer - Hpv16 RNA" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/webinars/from-small-rnas-to-chromosomes-an-overview-of-rnascope-technology-and-spatial-biology-tools" > <h3 class="card_title_htag"> <div><h3>Webinar: An Overview of RNAscope and Spatial Biology Tools </h3></div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/webinars/from-small-rnas-to-chromosomes-an-overview-of-rnascope-technology-and-spatial-biology-tools" > Watch Webinar </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/dnascope-chromogenic-in-situ-hybridization-assay-for-high-resolution-detection-of-dna-copy-number-and-structural-variations-whitepaper" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; 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