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Siganevich</title> <meta name="description" content="Search results for: Yana Siganevich"> <meta name="keywords" content="Yana Siganevich"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Yana Siganevich"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 14</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Yana Siganevich</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">14</span> Canadian Undergraduate and Graduate Nursing Students: Interest in Education in Medical and Recreational Cannabis for Practice and Career Development</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Margareth%20S.%20Zanchetta">Margareth S. Zanchetta</a>, <a href="https://publications.waset.org/abstracts/search?q=Kateryna%20Metersky"> Kateryna Metersky</a>, <a href="https://publications.waset.org/abstracts/search?q=Valerie%20Tan"> Valerie Tan</a>, <a href="https://publications.waset.org/abstracts/search?q=Charissa%20Cordon"> Charissa Cordon</a>, <a href="https://publications.waset.org/abstracts/search?q=Stephanie%20Lucchese"> Stephanie Lucchese</a>, <a href="https://publications.waset.org/abstracts/search?q=Yana%20Siganevich"> Yana Siganevich</a>, <a href="https://publications.waset.org/abstracts/search?q=Prasha%20Sivasundaram"> Prasha Sivasundaram</a>, <a href="https://publications.waset.org/abstracts/search?q=Truong%20Binh%20Nguyen"> Truong Binh Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Imran%20Qureshi"> Imran Qureshi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Due to a new area of practice, Canadian nurses possess knowledge gaps regarding the use of cannabis-based therapies by clients/patients. Education related to medical cannabis (MC) and recreational cannabis (RC) is required to promote nurses’ competency and confidence in supporting clients/patients using MC/RC toward the improvement of health outcomes. A team composed of nursing researchers and undergraduate/graduate students implemented a national survey to explore this theme with the population of undergraduate, graduate (MN and NP), and Post-Diploma (RN Bridging) nursing students enrolled in Canadian Universities Nursing Programs. Upon Research Ethics Board approval, survey recruitment was supported by major nursing stakeholders. The research questions were : (a) Which are the most preferred sources of information on MC/RC for nursing students? (b) Which are the factors and preferred learning modalities that could increase interest in learning about MC/RC, and (c) What are the future career plans among nursing students, and how would they consider the prospective use of cannabis in their practice? The survey was available from Sept. 2022 to Feb. 2023, hosted by a remote platform. An original questionnaire (English-French) was composed of 18 multiple choice questions and 2 open-ended questions. Sociodemographic information and closed-ended responses were compiled as descriptive statistics, while narrative accounts will be analysed through thematic analysis. Respondents (n=153) were from 7 Canadian provinces, national (99%) and international students (1%); the majority of respondents (61%) were in the age range of 21-30 years old. Results indicated that respondents perceive a gap in the undergraduate curriculum on the topics of MC/RC (91%) and that their learning needs include regulations (90%), data on effectiveness (88%), dosing best practices (86%), contraindications (83%), and clinical and medical indications (76%). Respondents reported motivation to learn more about MC/RC through online lectures/videos (65%), e-learning modules or online interactive training (61%), workshops (51%), webinars (36%), and social media (35%). Their primary career-related motivations regarding MC/RC knowledge include enhancing nursing practice (76%), learning about this growing scope of practice (61%), keeping up-to-date responding to scientific curiosity (59%), learning about evidence-based practice (59%), and utilizing alternative forms of medical treatment (37%). Respondents indicated that the integration of topics on cannabis in any course in the undergraduate and/or graduate curriculum would increase their desire to learn about MC/RC as equally as exposure within a clinical setting (75%). The emerging trend in the set of narrative responses (n=130) suggests that respondents believe educational MC/RC content should be integrated into core nursing courses. Respondents also urged educators to be well-informed about evidence-based practice related to MC/RC and to reflect upon stigma and biases surrounding its use. Future knowledge dissemination and translation activities include scholarly products and presentations to stimulate discussion amongst nursing faculty and students, as well as nurses in clinical settings. The goal is to mobilise talents and build collaboration for the development of a socially responsive curriculum on MC/RC competency to address the education-related expectations of all these social actors. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Canada" title="Canada">Canada</a>, <a href="https://publications.waset.org/abstracts/search?q=medical%20cannabis" title=" medical cannabis"> medical cannabis</a>, <a href="https://publications.waset.org/abstracts/search?q=nursing%20education" title=" nursing education"> nursing education</a>, <a href="https://publications.waset.org/abstracts/search?q=nursing%20graduate%20student" title=" nursing graduate student"> nursing graduate student</a>, <a href="https://publications.waset.org/abstracts/search?q=nursing%20undergraduate%20student" title=" nursing undergraduate student"> nursing undergraduate student</a>, <a href="https://publications.waset.org/abstracts/search?q=online%20survey" title=" online survey"> online survey</a>, <a href="https://publications.waset.org/abstracts/search?q=recreational%20cannabis" title=" recreational cannabis"> recreational cannabis</a> </p> <a href="https://publications.waset.org/abstracts/165257/canadian-undergraduate-and-graduate-nursing-students-interest-in-education-in-medical-and-recreational-cannabis-for-practice-and-career-development" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165257.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">90</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> Influence of Culturing Conditions on Biomass Yield, Total Lipid and Fatty Acid Composition of Some Filamentous Fungi</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alla%20V.%20Goncharova">Alla V. Goncharova</a>, <a href="https://publications.waset.org/abstracts/search?q=Tatyana%20A.%20Karpenyuk"> Tatyana A. Karpenyuk</a>, <a href="https://publications.waset.org/abstracts/search?q=Yana%20S.%20Tsurkan"> Yana S. Tsurkan</a>, <a href="https://publications.waset.org/abstracts/search?q=Rosa%20U.%20Beisembaeva"> Rosa U. Beisembaeva</a>, <a href="https://publications.waset.org/abstracts/search?q=Togzhan%20D.%20Mukasheva"> Togzhan D. Mukasheva</a>, <a href="https://publications.waset.org/abstracts/search?q=Ludmila%20V.%20Ignatova"> Ludmila V. Ignatova</a>, <a href="https://publications.waset.org/abstracts/search?q=Ramza%20Z.%20Berzhanova"> Ramza Z. Berzhanova </a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this work the effect of culturing conditions of filamentous fungi Penicillium raistrickii, Penicillium anatolicum, Fusarium sp. on biomass yield, the content of total lipids and fatty acids was studied. It has been established that in time the process of lipids accumulation correlated with biomass growth of cultures, reaching maximum values in stationary growth phase. Biomass yield and accumulation of general lipids was increased by adding zinc to the culture medium. The more intensive accumulation of biomass and general lipids was observed at temperature 18°C. Lowering the temperature of culturing has changed the ratio of saturated: Unsaturated fatty acids in the direction of increasing the latter. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biomass" title="biomass">biomass</a>, <a href="https://publications.waset.org/abstracts/search?q=culturing%20conditions" title=" culturing conditions"> culturing conditions</a>, <a href="https://publications.waset.org/abstracts/search?q=fungi" title=" fungi"> fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=fatty%20acids%20%28FA%29" title=" fatty acids (FA)"> fatty acids (FA)</a>, <a href="https://publications.waset.org/abstracts/search?q=growth%20dynamics" title=" growth dynamics"> growth dynamics</a>, <a href="https://publications.waset.org/abstracts/search?q=lipids" title=" lipids"> lipids</a> </p> <a href="https://publications.waset.org/abstracts/10193/influence-of-culturing-conditions-on-biomass-yield-total-lipid-and-fatty-acid-composition-of-some-filamentous-fungi" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10193.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">451</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> The Relationship between Anxiety and Willingness to Communicate: The Indonesian EFL Context</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yana%20Shanti%20Manipuspika">Yana Shanti Manipuspika</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Anxiety has potential to negatively affect foreign language learning process. This feeling leads the learners hesitate to communicate. This present study aimed at investigating the relationship between students’ anxiety and willingness to communicate of Indonesian EFL learners. There were 67 participants in this study who were the English Department students of Vocational Program of University of Brawijaya, Malang. This study employed Foreign Language Classroom Anxiety Scale (FLCAS) and the Willingness to Communicate (WTC) scale. The results of this study showed that the respondents had communication apprehension, test anxiety, and fear of negative evaluation. This study also revealed that English Department students of Vocational Program University of Brawijaya had high level of anxiety and low level of willingness to communicate. The relationship between foreign language classroom anxiety and willingness to communicate was found to be sufficiently negative. It is suggested for the language teachers to identify the causes of students’ language anxiety and try to create cheerful and less stressful atmosphere in the classroom. It is also important to find a way to develop their teaching strategies to stimulate students’ willingness to communicate. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=English%20as%20a%20foreign%20language%20%28EFL%29" title="English as a foreign language (EFL)">English as a foreign language (EFL)</a>, <a href="https://publications.waset.org/abstracts/search?q=foreign%20language%20classroom%20anxiety%20%28FLCA%29" title=" foreign language classroom anxiety (FLCA)"> foreign language classroom anxiety (FLCA)</a>, <a href="https://publications.waset.org/abstracts/search?q=vocational%20program" title=" vocational program"> vocational program</a>, <a href="https://publications.waset.org/abstracts/search?q=willingness%20to%20communicate%20%28WTC%29" title=" willingness to communicate (WTC)"> willingness to communicate (WTC)</a> </p> <a href="https://publications.waset.org/abstracts/71005/the-relationship-between-anxiety-and-willingness-to-communicate-the-indonesian-efl-context" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/71005.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">252</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">11</span> Oxytocin and Sensorimotor Synchronization in Pairs of Strangers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yana%20Gorina">Yana Gorina</a>, <a href="https://publications.waset.org/abstracts/search?q=Olga%20Lopatina"> Olga Lopatina</a>, <a href="https://publications.waset.org/abstracts/search?q=Elina%20Tsigeman"> Elina Tsigeman</a>, <a href="https://publications.waset.org/abstracts/search?q=Larisa%20Mararitsa"> Larisa Mararitsa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The ability to act in concert with others, the so-called sensorimotor synchronisation, is a fundamental human ability that underlies successful interpersonal coordination. The manifestation of accuracy and plasticity in synchronisation is an adaptive aspect of interaction with the environment, as well as the ability to predict upcoming actions and behaviour of others. The ability to temporarily coordinate one’s actions with a predictable external event is manifested in such types of social behaviour as a synchronised group dance to music played live by an orchestra, group sports (rowing, swimming, etc.), synchronised actions of surgeons during an operation, applause from an admiring audience, walking rhythms, etc. Both our body and mind are involved in achieving the synchronisation during social interactions. However, it has not yet been well described how the brain determine the external rhythm and what neuropeptides coordinate and synchronise actions. Over the past few decades, there has been an increased interest among neuroscientists and neurophysiologists regarding the neuropeptide oxytocin in the context of its complex, diverse and sometimes polar effects manifested in the emotional and social aspects of behaviour (attachment, trust, empathy, emotion recognition, stress response, anxiety and depression, etc.). Presumable, oxytocin might also be involved in social synchronisation processes. The aim of our study is to test the hypothesis that oxytocin is linked to interpersonal synchronisation in a pair of strangers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=behavior" title="behavior">behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=movement" title=" movement"> movement</a>, <a href="https://publications.waset.org/abstracts/search?q=oxytocin" title=" oxytocin"> oxytocin</a>, <a href="https://publications.waset.org/abstracts/search?q=synchronization" title=" synchronization"> synchronization</a> </p> <a href="https://publications.waset.org/abstracts/172103/oxytocin-and-sensorimotor-synchronization-in-pairs-of-strangers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/172103.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">62</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10</span> Influence of Culture Conditions on the Growth and Fatty Acid Composition of Green Microalgae Oocystis rhomboideus, Scenedesmus obliquus, Dictyochlorella globosa</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tatyana%20A.%20Karpenyuk">Tatyana A. Karpenyuk</a>, <a href="https://publications.waset.org/abstracts/search?q=Saltanat%20B.%20Orazova"> Saltanat B. Orazova</a>, <a href="https://publications.waset.org/abstracts/search?q=Yana%20S.%20Tzurkan"> Yana S. Tzurkan</a>, <a href="https://publications.waset.org/abstracts/search?q=Alla%20V.%20Goncharova"> Alla V. Goncharova</a>, <a href="https://publications.waset.org/abstracts/search?q=Bakytzhan%20K.%20Kairat"> Bakytzhan K. Kairat</a>, <a href="https://publications.waset.org/abstracts/search?q=Togzhan%20D.%20Mukasheva"> Togzhan D. Mukasheva</a>, <a href="https://publications.waset.org/abstracts/search?q=Ludmila%20V.%20Ignatova"> Ludmila V. Ignatova</a>, <a href="https://publications.waset.org/abstracts/search?q=Ramza%20Z.%20Berzhanova"> Ramza Z. Berzhanova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microalgae due to the ability to accumulate high levels of practically valuable polyunsaturated fatty acids attract attention as a promising raw material for commercial products. It were defined the features of the growth processes of cells green protococcal microalgae Oocystis rhomboideus, Scenedesmus obliquus, Dictyochlorella globosa at cultivation in different nutritional mediums. For the rapid accumulation of biomass, combined with high productivity of total lipids fraction yield recommended to use the Fitzgerald medium (Scenodesmus obliquus, Oocystis rhomboideus) and/or Bold medium (Dictyochlorella globosa). Productivity of lipids decreased in sequence Dictyochlorella globosa > Scenodesmus obliquus > Oocystis rhomboideus. The bulk of fatty acids fraction of the total lipids is unsaturated fatty acids, which accounts for 70 to 83% of the total number of fatty acids. The share of monoenic acids varies from 16 to 36 %, the share of unsaturated fatty acids - from 44 to 65% of total fatty acids fraction. Among the unsaturated acids dominate α-linolenic acid (C18:3n-3), hexadecatetraenic acid (C16:4) and linoleic acid (C18:2). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=microalgae" title="microalgae">microalgae</a>, <a href="https://publications.waset.org/abstracts/search?q=lipids" title=" lipids"> lipids</a>, <a href="https://publications.waset.org/abstracts/search?q=fatty%20acids" title=" fatty acids"> fatty acids</a>, <a href="https://publications.waset.org/abstracts/search?q=culture%20conditions" title=" culture conditions"> culture conditions</a> </p> <a href="https://publications.waset.org/abstracts/10149/influence-of-culture-conditions-on-the-growth-and-fatty-acid-composition-of-green-microalgae-oocystis-rhomboideus-scenedesmus-obliquus-dictyochlorella-globosa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10149.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">451</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Guilty Pleasure: The Relation between Marianismo and Latina’s Sexuality</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tania%20Camarillo%20Contreras">Tania Camarillo Contreras</a>, <a href="https://publications.waset.org/abstracts/search?q=Yana%20Kuchirko"> Yana Kuchirko</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Women’s sexuality, despite it being a core part of human functioning, has historically been stigmatized, silenced, and regulated and silenced across many cultures. The regulation of women’s bodies and desires has often been accomplished via gender ideologies and norms that ascribe power to men over women. Within Latino communities, gender ideologies like marianismo provide scripts by which women are expected to adhere submissiveness, purity, and putting others’ needs before their own. Prior work on sexuality-related topics among Latinas has almost exclusively examined the roles of risky behavior, partner violence, and gender roles on psychological and health outcomes. it know little about positive aspects of Latinas’ sexuality, such as their perspectives on sexual excitation and inhibition. Moreover, we know little about Latina’s sexuality in relation to marianismo. In this study, it ask the following questions (1) What are Latina’s perspectives on sexual excitation/inhibition? Do they vary by demographic variables (e.g., education, religiosity); (2) Does endorsement of marianismo among Latina women predict their perspectives on sexual excitation/sexual inhibition? and (3) Does endorsement of double standard (mediator variable) mediate the relation between marianismo (Independent) and sexual excitation/sexual inhibition? The results of this study shed light on effects of culturally specific gender ideologies like marianismo on women’s sexualities and have implications for culturally informed and gender-affirming policies seeking to better support Latinas’ sexual health. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cultural%20beliefs" title="cultural beliefs">cultural beliefs</a>, <a href="https://publications.waset.org/abstracts/search?q=latinas" title=" latinas"> latinas</a>, <a href="https://publications.waset.org/abstracts/search?q=marianismo" title=" marianismo"> marianismo</a>, <a href="https://publications.waset.org/abstracts/search?q=sexuality" title=" sexuality"> sexuality</a>, <a href="https://publications.waset.org/abstracts/search?q=sexual%20inhibition" title=" sexual inhibition"> sexual inhibition</a> </p> <a href="https://publications.waset.org/abstracts/172248/guilty-pleasure-the-relation-between-marianismo-and-latinas-sexuality" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/172248.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">70</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Design, Fabrication, and Study of Droplet Tube Based Triboelectric Nanogenerators</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yana%20Xiao">Yana Xiao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The invention of Triboelectric Nanogenerators (TENGs) provides an effective approach to the sustainable power of energy. Liquid-solid interfaces-based TENGs have been researched in virtue of less friction for harvesting energy from raindrops, rivers, and oceans in the form of water flows. However, TENGs based on droplets have rarely been investigated. In this study, we have proposed a new kind of droplet tube-based TENG (DT-TENG) with free-standing and reformative grating electrodes. Both straight and curved DT-TENGs were designed, fabricated, and evaluated, including straight tubes TENG with 27 electrodes and curved tubes TENG of 25cm radius curvature- at the inclination of 30°, 45° and 60° respectively. Different materials and hydrophobicity treatments for the tubes have also been studied, together with a discussion on the mechanism and applications of DT-TENGs. As different types of liquid discrepant energy performance, this kind of DT-TENG can be potentially used in laboratories to identify liquid or solvent. In addition, a smart fishing float is contrived, which can recognize different levels of movement speeds brought about by different weights and generate corresponding electric signals to remind the angler. The electric generation performance when using a PVC helix tube around a cylinder is similar in straight situations under the inclination of 45° in this experiment. This new structure changes the direction of a water drop or flows without losing kinetic energy, which makes utilizing Helix-Tube-TENG to harvest energy from different building morphologies possible. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=triboelectric%20nanogenerator" title="triboelectric nanogenerator">triboelectric nanogenerator</a>, <a href="https://publications.waset.org/abstracts/search?q=energy%20harvest" title=" energy harvest"> energy harvest</a>, <a href="https://publications.waset.org/abstracts/search?q=liquid%20tribomaterial" title=" liquid tribomaterial"> liquid tribomaterial</a>, <a href="https://publications.waset.org/abstracts/search?q=structure%20innovation" title=" structure innovation"> structure innovation</a> </p> <a href="https://publications.waset.org/abstracts/162127/design-fabrication-and-study-of-droplet-tube-based-triboelectric-nanogenerators" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162127.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">88</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> The Gut Microbiome in Cirrhosis and Hepatocellular Carcinoma: Characterization of Disease-Related Microbial Signature and the Possible Impact of Life Style and Nutrition</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lena%20Lapidot">Lena Lapidot</a>, <a href="https://publications.waset.org/abstracts/search?q=Amir%20Amnon"> Amir Amnon</a>, <a href="https://publications.waset.org/abstracts/search?q=Rita%20Nosenko"> Rita Nosenko</a>, <a href="https://publications.waset.org/abstracts/search?q=Veitsman%20Ella"> Veitsman Ella</a>, <a href="https://publications.waset.org/abstracts/search?q=Cohen-Ezra%20Oranit"> Cohen-Ezra Oranit</a>, <a href="https://publications.waset.org/abstracts/search?q=Davidov%20Yana"> Davidov Yana</a>, <a href="https://publications.waset.org/abstracts/search?q=Segev%20Shlomo"> Segev Shlomo</a>, <a href="https://publications.waset.org/abstracts/search?q=Koren%20Omry"> Koren Omry</a>, <a href="https://publications.waset.org/abstracts/search?q=Safran%20Michal"> Safran Michal</a>, <a href="https://publications.waset.org/abstracts/search?q=Ben-Ari%20Ziv"> Ben-Ari Ziv</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer related mortality worldwide. Liver Cirrhosis is the main predisposing risk factor for the development of HCC. The factor(s) influencing disease progression from Cirrhosis to HCC remain unknown. Gut microbiota has recently emerged as a major player in different liver diseases, however its association with HCC is still a mystery. Moreover, there might be an important association between the gut microbiota, nutrition, life style and the progression of Cirrhosis and HCC. The aim of our study was to characterize the gut microbial signature in association with life style and nutrition of patients with Cirrhosis, HCC-Cirrhosis and healthy controls. Design: Stool samples were collected from 95 individuals (30 patients with HCC, 38 patients with Cirrhosis and 27 age, gender and BMI-matched healthy volunteers). All participants answered lifestyle and Food Frequency Questionnaires. 16S rRNA sequencing of fecal DNA was performed (MiSeq Illumina). Results: There was a significant decrease in alpha diversity in patients with Cirrhosis (qvalue=0.033) and in patients with HCC-Cirrhosis (qvalue=0.032) compared to healthy controls. The microbiota of patients with HCC-cirrhosis compared to patients with Cirrhosis, was characterized by a significant overrepresentation of Clostridium (pvalue=0.024) and CF231 (pvalue=0.010) and lower expression of Alphaproteobacteria (pvalue=0.039) and Verrucomicrobia (pvalue=0.036) in several taxonomic levels: Verrucomicrobiae, Verrucomicrobiales, Verrucomicrobiaceae and the genus Akkermansia (pvalue=0.039). Furthermore, we performed an analysis of predicted metabolic pathways (Kegg level 2) that resulted in a significant decrease in the diversity of metabolic pathways in patients with HCC-Cirrhosis (qvalue=0.015) compared to controls, one of which was amino acid metabolism. Furthermore, investigating the life style and nutrition habits of patients with HCC-Cirrhosis, we found significant correlations between intake of artificial sweeteners and Verrucomicrobia (qvalue=0.12), High sugar intake and Synergistetes (qvalue=0.021) and High BMI and the pathogen Campylobacter (qvalue=0.066). Furthermore, overweight in patients with HCC-Cirrhosis modified bacterial diversity (qvalue=0.023) and composition (qvalue=0.033). Conclusions: To the best of the our knowledge, we present the first report of the gut microbial composition in patients with HCC-Cirrhosis, compared with Cirrhotic patients and healthy controls. We have demonstrated in our study that there are significant differences in the gut microbiome of patients with HCC-cirrhosis compared to Cirrhotic patients and healthy controls. Our findings are even more pronounced because the significantly increased bacteria Clostridium and CF231 in HCC-Cirrhosis weren't influenced by diet and lifestyle, implying this change is due to the development of HCC. Further studies are needed to confirm these findings and assess causality. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cirrhosis" title="Cirrhosis">Cirrhosis</a>, <a href="https://publications.waset.org/abstracts/search?q=Hepatocellular%20carcinoma" title=" Hepatocellular carcinoma"> Hepatocellular carcinoma</a>, <a href="https://publications.waset.org/abstracts/search?q=life%20style" title=" life style"> life style</a>, <a href="https://publications.waset.org/abstracts/search?q=liver%20disease" title=" liver disease"> liver disease</a>, <a href="https://publications.waset.org/abstracts/search?q=microbiome" title=" microbiome"> microbiome</a>, <a href="https://publications.waset.org/abstracts/search?q=nutrition" title=" nutrition"> nutrition</a> </p> <a href="https://publications.waset.org/abstracts/96178/the-gut-microbiome-in-cirrhosis-and-hepatocellular-carcinoma-characterization-of-disease-related-microbial-signature-and-the-possible-impact-of-life-style-and-nutrition" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96178.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">129</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Effect of Vitrification on Embryos Euploidy Obtained from Thawed Oocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Buderatskaya">Natalia Buderatskaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Igor%20Ilyin"> Igor Ilyin</a>, <a href="https://publications.waset.org/abstracts/search?q=Julia%20Gontar"> Julia Gontar</a>, <a href="https://publications.waset.org/abstracts/search?q=Sergey%20Lavrynenko"> Sergey Lavrynenko</a>, <a href="https://publications.waset.org/abstracts/search?q=Olga%20Parnitskaya"> Olga Parnitskaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Ekaterina%20Ilyina"> Ekaterina Ilyina</a>, <a href="https://publications.waset.org/abstracts/search?q=Eduard%20Kapustin"> Eduard Kapustin</a>, <a href="https://publications.waset.org/abstracts/search?q=Yana%20Lakhno"> Yana Lakhno</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: It is known that cryopreservation of oocytes has peculiar features due to the complex structure of the oocyte. One of the most important features is that mature oocytes contain meiotic division spindle which is very sensitive even to the slightest variation in temperature. Thus, the main objective of this study is to analyse the resulting euploid embryos obtained from thawed oocytes in comparison with the data of preimplantation genetic screening (PGS) in fresh embryo cycles. Material and Methods: The study was conducted at 'Medical Centre IGR' from January to July 2016. Data were analysed for 908 donor oocytes obtained in 67 cycles of assisted reproductive technologies (ART), of which 693 oocytes were used in the 51 'fresh' cycles (group A), and 215 oocytes - 16 ART programs with vitrification female gametes (group B). The average age of donors in the groups match 27.3±2.9 and 27.8±6.6 years. Stimulation of superovulation was conducted the standard way. Vitrification was performed in 1-2 hours after transvaginal puncture and thawing of oocytes were carried out in accordance with the standard protocol of Cryotech (Japan). Manipulation ICSI was performed 4-5 hours after transvaginal follicle puncture for fresh oocytes, or after defrosting - for vitrified female gametes. For the PGS, an embryonic biopsy was done on the third or on the fifth day after fertilization. Diagnostic procedures were performed using fluorescence in situ hybridization with the study of such chromosomes as 13, 16, 18, 21, 22, X, Y. Only morphologically quality blastocysts were used for the transfer, the estimation of which corresponded to the Gardner criteria. The statistical hypotheses were done using the criteria t, x^2 at a significance levels p<0.05, p<0.01, p<0.001. Results: The mean number of mature oocytes per cycle in group A was 13.58±6.65 and in group B - 13.44±6.68 oocytes for patient. The survival of oocytes after thawing totaled 95.3% (n=205), which indicates a highly effective quality of performed vitrification. The proportion of zygotes in the group A corresponded to 91.1%(n=631), in the group B – 80.5%(n=165), which shows statistically significant difference between the groups (p<0.001) and explained by non-viable oocytes elimination after vitrification. This is confirmed by the fact that on the fifth day of embryos development a statistically significant difference in the number of blastocysts was absent (p>0.05), and constituted respectively 61.6%(n=389) and 63.0%(n=104) in the groups. For the PGS performing 250 embryos analyzed in the group A and 72 embryos - in the group B. The results showed that euploidy in the studied chromosomes were 40.0%(n=100) embryos in the group A and 41.7% (n=30) - in the group B, which shows no statistical significant difference (p>0.05). The indicators of clinical pregnancies in the groups amounted to 64.7% (22 pregnancies per 34 embryo transfers) and 61.5% (8 pregnancies per 13 embryo transfers) respectively, and also had no significant difference between the groups (p>0.05). Conclusions: The results showed that the vitrification does not affect the resulting euploid embryos in assisted reproductive technologies and are not reflected in their morphological characteristics in ART programs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=euploid%20embryos" title="euploid embryos">euploid embryos</a>, <a href="https://publications.waset.org/abstracts/search?q=preimplantation%20genetic%20screening" title=" preimplantation genetic screening"> preimplantation genetic screening</a>, <a href="https://publications.waset.org/abstracts/search?q=thawing%20oocytes" title=" thawing oocytes"> thawing oocytes</a>, <a href="https://publications.waset.org/abstracts/search?q=vitrification" title=" vitrification"> vitrification</a> </p> <a href="https://publications.waset.org/abstracts/57504/effect-of-vitrification-on-embryos-euploidy-obtained-from-thawed-oocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57504.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">334</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Analysis of the Blastocysts Chromosomal Set Obtained after the Use of Donor Oocyte Cytoplasmic Transfer Technology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Julia%20Gontar">Julia Gontar</a>, <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Buderatskaya"> Natalia Buderatskaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Igor%20Ilyin"> Igor Ilyin</a>, <a href="https://publications.waset.org/abstracts/search?q=Olga%20Parnitskaya"> Olga Parnitskaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Sergey%20Lavrynenko"> Sergey Lavrynenko</a>, <a href="https://publications.waset.org/abstracts/search?q=Eduard%20Kapustin"> Eduard Kapustin</a>, <a href="https://publications.waset.org/abstracts/search?q=Ekaterina%20Ilyina"> Ekaterina Ilyina</a>, <a href="https://publications.waset.org/abstracts/search?q=Yana%20Lakhno"> Yana Lakhno</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: It is well known that oocytes obtained from older reproductive women have accumulated mitochondrial DNA mutations, which negatively affects the morphology of a developing embryo and may lead to the birth of a child with mitochondrial disease. Special techniques have been developed to allow a donor oocyte cytoplasmic transfer with the parents’ biological nuclear DNA retention. At the same time, it is important to understand whether the procedure affects the future embryonic chromosome sets as the nuclear DNA is the transfer subject in this new complex procedure. Material and Methods: From July 2015 to July 2016, the investigation was carried out in the Medical Centre IGR. 34 donor oocytes (group A) were used for the manipulation with the aim of donating cytoplasm: 21 oocytes were used for zygotes pronuclear transfer and oocytes 13 – for the spindle transfer. The mean age of the oocyte donors was 28.4±2.9 years. The procedure was performed using Nikon Ti Eclipse inverted microscope equipped with the micromanipulators Narishige system (Japan), Saturn 3 laser console (UK), Oosight imaging systems (USA). For the preimplantation genetic screening (PGS) blastocyst biopsy was performed, trophectoderm samples were diagnosed using fluorescent in situ hybridization on chromosomes 9, 13, 15, 16, 17, 18, 21, 22, X, Y. For comparison of morphological characteristics and euploidy, was chosen a group of embryos (group B) with the amount of 121 blastocysts obtained from 213 oocytes, which were gotten from the donor programs of assisted reproductive technologies (ART). Group B was not subjected to donor oocyte cytoplasmic transfer procedure and studied on the above mentioned chromosomes. Statistical analysis was carried out using the criteria t, x^2 at a significance levels p<0.05, p<0.01, p<0.001. Results: After the donor cytoplasm transfer process the amount of the third day developing embryos was 27 (79.4%). In this stage, the group B consisted of 189 (88.7%) developing embryos, and there was no statistically significant difference (SSD) between the two groups (p>0.05). After a comparative analysis of the morphological characteristics of the embryos on the fifth day, we also found no SSD among the studied groups (p>0.05): from 34 oocytes exposed to manipulation, 14 (41.2%) blastocysts was obtained, while the group B blastocyst yield was 56.8% (n=121) from 213 oocytes. The following results were obtained after PGS performing: in group A euploidy in studied chromosomes were 28.6%(n=4) blastocysts, whereas in group B this rate was 40.5%(n=49), 28.6%(n=4) and 21.5%(n=26) of mosaic embryos and 42.8%(n=6) and 38.0%(n=46) aneuploid blastocysts respectively were identified. None of these specified parameters had an SSD (p>0.05). But attention was drawn by the blastocysts in group A with identified mosaicism, which was chaotic without any cell having euploid chromosomal set, in contrast to the mosaic embryos in group B where identified chaotic mosaicism was only 2.5%(n=3). Conclusions: According to the obtained results, there is no direct procedural effect on the chromosome in embryos obtained following donor oocyte cytoplasmic transfer. Thus, the technology introduction will enhance the infertility treating effectiveness as well as avoiding having a child with mitochondrial disease. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=donor%20oocyte%20cytoplasmic%20transfer" title="donor oocyte cytoplasmic transfer">donor oocyte cytoplasmic transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=embryos%E2%80%99%20chromosome%20set" title=" embryos’ chromosome set"> embryos’ chromosome set</a>, <a href="https://publications.waset.org/abstracts/search?q=oocyte%20spindle%20transfer" title=" oocyte spindle transfer"> oocyte spindle transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=pronuclear%20transfer" title=" pronuclear transfer"> pronuclear transfer</a> </p> <a href="https://publications.waset.org/abstracts/57502/analysis-of-the-blastocysts-chromosomal-set-obtained-after-the-use-of-donor-oocyte-cytoplasmic-transfer-technology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57502.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">328</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Analysis of Mutation Associated with Male Infertility in Patients and Healthy Males in the Russian Population</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Svetlana%20Zhikrivetskaya">Svetlana Zhikrivetskaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Nataliya%20Shirokova"> Nataliya Shirokova</a>, <a href="https://publications.waset.org/abstracts/search?q=Roman%20Bikanov"> Roman Bikanov</a>, <a href="https://publications.waset.org/abstracts/search?q=Elizaveta%20Musatova"> Elizaveta Musatova</a>, <a href="https://publications.waset.org/abstracts/search?q=Yana%20Kovaleva"> Yana Kovaleva</a>, <a href="https://publications.waset.org/abstracts/search?q=Nataliya%20Vetrova"> Nataliya Vetrova</a>, <a href="https://publications.waset.org/abstracts/search?q=Ekaterina%20Pomerantseva"> Ekaterina Pomerantseva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Nowadays there is a growing number of couples with conceiving problems due to male or female infertility. Genetic abnormalities are responsible for about 31% of all cases of male infertility. These abnormalities include both chromosomal aberrations or aneuploidies and mutations in certain genes. Chromosomal abnormalities can be easily identified, thus the development of screening panels able to reveal genetic reasons of male infertility on gene level is of current interest. There are approximately 2,000 genes involved in male fertility that is the reason why it is very important to determine the most clinically relevant in certain population and ethnic conditions. An infertility screening panel containing 48 mutations in genes AMHR2, CFTR, DNAI1, HFE, KAL1, TSSK2 and AZF locus which are the most clinically relevant for the European population according to databases NCBI and ClinVar was designed. The aim of this research was to confirm clinic relevance of these mutations in the Russian population. Genotyping was performed in 220 patients with different types of male infertility and in 57 healthy males with normozoospermia. Mutations were identified by end-point PCR with TaqMan probes in microfluidic plates. The frequency of 5 mutations in healthy males and 13 mutations in patients with infertility was revealed and estimated. The frequency of mutation c.187C>G in HFE gene was significantly lower for healthy males (8.8%) compared with patients (17.7%) and the values for the European population according to ExAc database (13.7%) and dbSNP (17.2%). Analysis of c.3454G>C, and c.1545_1546delTA mutations in the CFTR gene revealed increased frequency (0.9 and 0.2%, respectively) in patients with infertility compared with data for the European population (0.04%, respectively (ExAc, European (Non-Finnish) and for the Aggregated Populations (0.002% (ExAc), because there is no data for European population for c.1545_1546delTA mutation. The frequency of del508 mutation (CFTR) in patients (1.59%) were lower comparing with male infertility Europeans (3.34-6.25% depending on nationality) and at the same level with healthy Europeans (1.06%, ExAc, European (Non-Finnish). Analysis of c.845G>A (HFE) mutation resulted in decreased frequency in patients (1.8%) in contrast with the European population data (5.1%, respectively, ExAc, European (Non-Finnish). Moreover, obtained data revealed no statistically significant frequency difference for c.845G>A mutation (HFE) between healthy males in the Russian and the European populations. Allele frequencies of mutations c.350G>A (CFTR), c.193A>T (HFE), c.774C>T, and c.80A>G (gene TSSK2) showed no significantly difference among patients with infertility, healthy males and Europeans. Analysis of AZF locus revealed increased frequency for AZFc microdeletion in patients with male infertility. Thereby, the new data of the allele frequencies in infertility patients in the Russian population was obtained. As well as the frequency differences of mutations associated with male infertility among patients, healthy males in the Russian population and the European one were estimated. The revealed differences showed that for high effectiveness of screening panel detecting genetically caused male infertility it is very important to consider ethnic and population characteristics of patients which will be screened. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=allele%20frequency" title="allele frequency">allele frequency</a>, <a href="https://publications.waset.org/abstracts/search?q=azoospermia" title=" azoospermia"> azoospermia</a>, <a href="https://publications.waset.org/abstracts/search?q=male%20infertility" title=" male infertility"> male infertility</a>, <a href="https://publications.waset.org/abstracts/search?q=mutation" title=" mutation"> mutation</a>, <a href="https://publications.waset.org/abstracts/search?q=population" title=" population"> population</a> </p> <a href="https://publications.waset.org/abstracts/59512/analysis-of-mutation-associated-with-male-infertility-in-patients-and-healthy-males-in-the-russian-population" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59512.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">392</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Design, Development and Testing of Polymer-Glass Microfluidic Chips for Electrophoretic Analysis of Biological Sample</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yana%20Posmitnaya">Yana Posmitnaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Galina%20Rudnitskaya"> Galina Rudnitskaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Tatyana%20Lukashenko"> Tatyana Lukashenko</a>, <a href="https://publications.waset.org/abstracts/search?q=Anton%20Bukatin"> Anton Bukatin</a>, <a href="https://publications.waset.org/abstracts/search?q=Anatoly%20Evstrapov"> Anatoly Evstrapov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An important area of biological and medical research is the study of genetic mutations and polymorphisms that can alter gene function and cause inherited diseases and other diseases. The following methods to analyse DNA fragments are used: capillary electrophoresis and electrophoresis on microfluidic chip (MFC), mass spectrometry with electrophoresis on MFC, hybridization assay on microarray. Electrophoresis on MFC allows to analyse small volumes of samples with high speed and throughput. A soft lithography in polydimethylsiloxane (PDMS) was chosen for operative fabrication of MFCs. A master-form from silicon and photoresist SU-8 2025 (MicroChem Corp.) was created for the formation of micro-sized structures in PDMS. A universal topology which combines T-injector and simple cross was selected for the electrophoretic separation of the sample. Glass K8 and PDMS Sylgard® 184 (Dow Corning Corp.) were used for fabrication of MFCs. Electroosmotic flow (EOF) plays an important role in the electrophoretic separation of the sample. Therefore, the estimate of the quantity of EOF and the ways of its regulation are of interest for the development of the new methods of the electrophoretic separation of biomolecules. The following methods of surface modification were chosen to change EOF: high-frequency (13.56 MHz) plasma treatment in oxygen and argon at low pressure (1 mbar); 1% aqueous solution of polyvinyl alcohol; 3% aqueous solution of Kolliphor® P 188 (Sigma-Aldrich Corp.). The electroosmotic mobility was evaluated by the method of Huang X. et al., wherein the borate buffer was used. The influence of physical and chemical methods of treatment on the wetting properties of the PDMS surface was controlled by the sessile drop method. The most effective way of surface modification of MFCs, from the standpoint of obtaining the smallest value of the contact angle and the smallest value of the EOF, was the processing with aqueous solution of Kolliphor® P 188. This method of modification has been selected for the treatment of channels of MFCs, which are used for the separation of mixture of oligonucleotides fluorescently labeled with the length of chain with 10, 20, 30, 40 and 50 nucleotides. Electrophoresis was performed on the device MFAS-01 (IAI RAS, Russia) at the separation voltage of 1500 V. 6% solution of polydimethylacrylamide with the addition of 7M carbamide was used as the separation medium. The separation time of components of the mixture was determined from electropherograms. The time for untreated MFC was ~275 s, and for the ones treated with solution of Kolliphor® P 188 – ~ 220 s. Research of physical-chemical methods of surface modification of MFCs allowed to choose the most effective way for reducing EOF – the modification with aqueous solution of Kolliphor® P 188. In this case, the separation time of the mixture of oligonucleotides decreased about 20%. The further optimization of method of modification of channels of MFCs will allow decreasing the separation time of sample and increasing the throughput of analysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electrophoresis" title="electrophoresis">electrophoresis</a>, <a href="https://publications.waset.org/abstracts/search?q=microfluidic%20chip" title=" microfluidic chip"> microfluidic chip</a>, <a href="https://publications.waset.org/abstracts/search?q=modification" title=" modification"> modification</a>, <a href="https://publications.waset.org/abstracts/search?q=nucleic%20acid" title=" nucleic acid"> nucleic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=polydimethylsiloxane" title=" polydimethylsiloxane"> polydimethylsiloxane</a>, <a href="https://publications.waset.org/abstracts/search?q=soft%20lithography" title=" soft lithography"> soft lithography</a> </p> <a href="https://publications.waset.org/abstracts/24584/design-development-and-testing-of-polymer-glass-microfluidic-chips-for-electrophoretic-analysis-of-biological-sample" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24584.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">413</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> EEG and DC-Potential Level Сhanges in the Elderly</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Irina%20Deputat">Irina Deputat</a>, <a href="https://publications.waset.org/abstracts/search?q=Anatoly%20Gribanov"> Anatoly Gribanov</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuliya%20Dzhos"> Yuliya Dzhos</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexandra%20Nekhoroshkova"> Alexandra Nekhoroshkova</a>, <a href="https://publications.waset.org/abstracts/search?q=Tatyana%20Yemelianova"> Tatyana Yemelianova</a>, <a href="https://publications.waset.org/abstracts/search?q=Irina%20Bolshevidtseva"> Irina Bolshevidtseva</a>, <a href="https://publications.waset.org/abstracts/search?q=Irina%20Deryabina"> Irina Deryabina</a>, <a href="https://publications.waset.org/abstracts/search?q=Yana%20Kereush"> Yana Kereush</a>, <a href="https://publications.waset.org/abstracts/search?q=Larisa%20Startseva"> Larisa Startseva</a>, <a href="https://publications.waset.org/abstracts/search?q=Tatyana%20Bagretsova"> Tatyana Bagretsova</a>, <a href="https://publications.waset.org/abstracts/search?q=Irina%20Ikonnikova"> Irina Ikonnikova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the modern world the number of elderly people increases. Preservation of functionality of an organism in the elderly becomes very important now. During aging the higher cortical functions such as feelings, perception, attention, memory, and ideation are gradual decrease. It is expressed in the rate of information processing reduction, volume of random access memory loss, ability to training and storing of new information decrease. Perspective directions in studying of aging neurophysiological parameters are brain imaging: computer electroencephalography, neuroenergy mapping of a brain, and also methods of studying of a neurodynamic brain processes. Research aim – to study features of a brain aging in elderly people by electroencephalogram (EEG) and the DC-potential level. We examined 130 people aged 55 - 74 years that did not have psychiatric disorders and chronic states in a decompensation stage. EEG was recorded with a 128-channel GES-300 system (USA). EEG recordings are collected while the participant sits at rest with their eyes closed for 3 minutes. For a quantitative assessment of EEG we used the spectral analysis. The range was analyzed on delta (0,5–3,5 Hz), a theta - (3,5–7,0 Hz), an alpha 1-(7,0–11,0 Hz) an alpha 2-(11–13,0 Hz), beta1-(13–16,5 Hz) and beta2-(16,5–20 Hz) ranges. In each frequency range spectral power was estimated. The 12-channel hardware-software diagnostic ‘Neuroenergometr-KM’ complex was applied for registration, processing and the analysis of a brain constant potentials level. The DC-potential level registered in monopolar leads. It is revealed that the EEG of elderly people differ in higher rates of spectral power in the range delta (р < 0,01) and a theta - (р < 0,05) rhythms, especially in frontal areas in aging. By results of the comparative analysis it is noted that elderly people 60-64 aged differ in higher values of spectral power alfa-2 range in the left frontal and central areas (р < 0,05) and also higher values beta-1 range in frontal and parieto-occipital areas (р < 0,05). Study of a brain constant potential level distribution revealed increase of total energy consumption on the main areas of a brain. In frontal leads we registered the lowest values of constant potential level. Perhaps it indicates decrease in an energy metabolism in this area and difficulties of executive functions. The comparative analysis of a potential difference on the main assignments testifies to unevenness of a lateralization of a brain functions at elderly people. The results of a potential difference between right and left hemispheres testify to prevalence of the left hemisphere activity. Thus, higher rates of functional activity of a cerebral cortex are peculiar to people of early advanced age (60-64 years) that points to higher reserve opportunities of central nervous system. By 70 years there are age changes of a cerebral power exchange and level of electrogenesis of a brain which reflect deterioration of a condition of homeostatic mechanisms of self-control and the program of processing of the perceptual data current flow. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=brain" title="brain">brain</a>, <a href="https://publications.waset.org/abstracts/search?q=DC-potential%20level" title=" DC-potential level"> DC-potential level</a>, <a href="https://publications.waset.org/abstracts/search?q=EEG" title=" EEG"> EEG</a>, <a href="https://publications.waset.org/abstracts/search?q=elderly%20people" title=" elderly people "> elderly people </a> </p> <a href="https://publications.waset.org/abstracts/35367/eeg-and-dc-potential-level-shanges-in-the-elderly" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35367.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">484</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> An Integrated Approach to the Carbonate Reservoir Modeling: Case Study of the Eastern Siberia Field</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yana%20Snegireva">Yana Snegireva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Carbonate reservoirs are known for their heterogeneity, resulting from various geological processes such as diagenesis and fracturing. These complexities may cause great challenges in understanding fluid flow behavior and predicting the production performance of naturally fractured reservoirs. The investigation of carbonate reservoirs is crucial, as many petroleum reservoirs are naturally fractured, which can be difficult due to the complexity of their fracture networks. This can lead to geological uncertainties, which are important for global petroleum reserves. The problem outlines the key challenges in carbonate reservoir modeling, including the accurate representation of fractures and their connectivity, as well as capturing the impact of fractures on fluid flow and production. Traditional reservoir modeling techniques often oversimplify fracture networks, leading to inaccurate predictions. Therefore, there is a need for a modern approach that can capture the complexities of carbonate reservoirs and provide reliable predictions for effective reservoir management and production optimization. The modern approach to carbonate reservoir modeling involves the utilization of the hybrid fracture modeling approach, including the discrete fracture network (DFN) method and implicit fracture network, which offer enhanced accuracy and reliability in characterizing complex fracture systems within these reservoirs. This study focuses on the application of the hybrid method in the Nepsko-Botuobinskaya anticline of the Eastern Siberia field, aiming to prove the appropriateness of this method in these geological conditions. The DFN method is adopted to model the fracture network within the carbonate reservoir. This method considers fractures as discrete entities, capturing their geometry, orientation, and connectivity. But the method has significant disadvantages since the number of fractures in the field can be very high. Due to limitations in the amount of main memory, it is very difficult to represent these fractures explicitly. By integrating data from image logs (formation micro imager), core data, and fracture density logs, a discrete fracture network (DFN) model can be constructed to represent fracture characteristics for hydraulically relevant fractures. The results obtained from the DFN modeling approaches provide valuable insights into the East Siberia field's carbonate reservoir behavior. The DFN model accurately captures the fracture system, allowing for a better understanding of fluid flow pathways, connectivity, and potential production zones. The analysis of simulation results enables the identification of zones of increased fracturing and optimization opportunities for reservoir development with the potential application of enhanced oil recovery techniques, which were considered in further simulations on the dual porosity and dual permeability models. This approach considers fractures as separate, interconnected flow paths within the reservoir matrix, allowing for the characterization of dual-porosity media. The case study of the East Siberia field demonstrates the effectiveness of the hybrid model method in accurately representing fracture systems and predicting reservoir behavior. The findings from this study contribute to improved reservoir management and production optimization in carbonate reservoirs with the use of enhanced and improved oil recovery methods. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=carbonate%20reservoir" title="carbonate reservoir">carbonate reservoir</a>, <a href="https://publications.waset.org/abstracts/search?q=discrete%20fracture%20network" title=" discrete fracture network"> discrete fracture network</a>, <a href="https://publications.waset.org/abstracts/search?q=fracture%20modeling" title=" fracture modeling"> fracture modeling</a>, <a href="https://publications.waset.org/abstracts/search?q=dual%20porosity" title=" dual porosity"> dual porosity</a>, <a href="https://publications.waset.org/abstracts/search?q=enhanced%20oil%20recovery" title=" enhanced oil recovery"> enhanced oil recovery</a>, <a href="https://publications.waset.org/abstracts/search?q=implicit%20fracture%20model" title=" implicit fracture model"> implicit fracture model</a>, <a href="https://publications.waset.org/abstracts/search?q=hybrid%20fracture%20model" title=" hybrid fracture model"> hybrid fracture model</a> </p> <a href="https://publications.waset.org/abstracts/168369/an-integrated-approach-to-the-carbonate-reservoir-modeling-case-study-of-the-eastern-siberia-field" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/168369.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">75</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a 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