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field--name-field-text field--type-text-long field--label-hidden field__items"> <p>Histone acetyltransferase assays performed at Reaction Biology are radiometric activity assays using tritiated acetyl-Coenzyme A as a cofactor.</p> <p>Reaction Biology’s HAT assays are radioisotope-based filtration assays. This gold standard approach can be used regardless of whether the substrate is a peptide, histone, nucleosome, or non-histone protein. A radiolabeled acetyl group is transferred to the substrate to quantify enzyme activity.</p> <p>With ~1750 lysine-acetylated proteins in the human proteome, Reaction Biology’s histone acetyltransferase assays provide an important advantage in terms of assay flexibility as well as gold standard quality.</p> <ul> <li>Direct and accurate measurement of <span lang="EN-US" xml:lang="EN-US">histone acetyltransferase activity</span></li> <li>Low scale, large scale, and high-throughput HAT inhibitor screening options</li> <li>Histone acetyltransferase assay development and research collaboration options</li> </ul> <p><span lang="EN-US" xml:lang="EN-US">All our histone acetyltransferase assay services are performed at our screening facility in Pennsylvania, US.</span> Our team of Ph.D. scientists provide quality data and excellent communication, for a superior research result.</p> <p><span lang="EN-US" xml:lang="EN-US">Business development managers in your area answer to your HAT assay screening inquiries to support your specific research needs. 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paragraph--view-mode--default"><div id="haah-id" class="paragraph paragraph--type--table-one paragraph--view-mode--default"><div class="container center"><h2>List of HAT Activity Assays (9)</h2> <div class="field field--name-field-table-one field--type-tablefield field--label-hidden field__item"> <div id="tablefield-wrapper-paragraph-2716-field_table_one-0" class="tablefield-wrapper"> <div id="tablefield-paragraph-2716-field_table_one-0_wrapper" class="dataTables_wrapper no-footer"> <table id="tablefield-paragraph-2716-field_table_one-0" class="tablefield dataTable no-footer dtr-inline" role="grid" data-striping="1"> <thead> <tr role="row"> <th class="row_0 col_0 all control sorting_asc" tabindex="0" colspan="1" rowspan="1" aria-controls="tablefield-paragraph-2716-field_table_one-0" aria-sort="ascending" aria-label="Target: activate to sort column descending">Target</th> <th class="row_0 col_1 min-fablet sorting" tabindex="0" colspan="1" rowspan="1" aria-controls="tablefield-paragraph-2716-field_table_one-0" aria-label="Synonyms: activate to sort column ascending">Synonyms</th> <th class="row_0 col_2 min-fablet sorting" tabindex="0" colspan="1" rowspan="1" aria-controls="tablefield-paragraph-2716-field_table_one-0" aria-label="UniProt: activate to sort column ascending">UniProt</th> <th class="row_0 col_3 min-tablet sorting" tabindex="0" colspan="1" rowspan="1" aria-controls="tablefield-paragraph-2716-field_table_one-0" aria-label="Data: activate to sort column ascending">Data</th> </tr> </thead> <tbody> <tr class="odd" role="row"> <td class="row_1 col_0 all control sorting_1" tabindex="0">CBP</td> <td class="row_1 col_1 min-fablet">CREBBP</td> <td class="row_1 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q92793">Q92793</a></td> <td class="row_1 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/cbp_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_2 col_0 all control sorting_1" tabindex="0">KAT2A</td> <td class="row_2 col_1 min-fablet">hGCN5</td> <td class="row_2 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q92830">Q92830</a></td> <td class="row_2 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/hgcn5_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_3 col_0 all control sorting_1" tabindex="0">KAT2B</td> <td class="row_3 col_1 min-fablet">pCAF</td> <td class="row_3 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q92831">Q92831</a></td> <td class="row_3 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pcaf_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_4 col_0 all control sorting_1" tabindex="0">KAT5</td> <td class="row_4 col_1 min-fablet">TIP60</td> <td class="row_4 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q92993">Q92993</a></td> <td class="row_4 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/kat5_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_5 col_0 all control sorting_1" tabindex="0">KAT6A</td> <td class="row_5 col_1 min-fablet">MYST3</td> <td class="row_5 col_2 link_text min-fablet"><a class="link_text" href="https://www.uniprot.org/uniprotkb/Q92794">Q92794</a></td> <td class="row_5 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/kat6a_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_6 col_0 all control sorting_1" tabindex="0">KAT6B</td> <td class="row_6 col_1 min-fablet">MYST4</td> <td class="row_6 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q8WYB5">Q8WYB5</a></td> <td class="row_6 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/myst4_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_7 col_0 all control sorting_1" tabindex="0">KAT7</td> <td class="row_7 col_1 min-fablet">MYST2</td> <td class="row_7 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/O95251">O95251</a></td> <td class="row_7 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/myst2_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_8 col_0 all control sorting_1" tabindex="0">KAT8</td> <td class="row_8 col_1 min-fablet">MYST1</td> <td class="row_8 col_2 link_text min-fablet"><a class="link_text" href="https://www.uniprot.org/uniprotkb/Q9H7Z6">Q9H7Z6</a></td> <td class="row_8 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/kat8_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_9 col_0 all control sorting_1" tabindex="0">p300</td> <td class="row_9 col_1 min-fablet">EP300</td> <td class="row_9 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q09472">Q09472</a></td> <td class="row_9 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/p300_hat_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> </tbody> </table> </div> </div> </div> </div></div><div id="haah-id2" class="paragraph paragraph--type--tab-container paragraph--view-mode--default"><div class="container center"><h2>HAT Inhibitor Screening Details</h2> <div class="tab-container"><ul class="tab-navigation"><li class="tab-active" data-id="#tab-haah-id221">Assay format </li><li data-id="#tab-haah-id222">Assay setup </li></ul><div class="field field--name-field-paragraph field--type-entity-reference-revisions field--label-hidden field__items"><div id="tab-haah-id221" class="tab-content-active paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Assay format </div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><div class="field__item"><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-1294" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/191220_Mehdi_HAT_assay_principle.png" alt="" width="5115" height="674" srcset="https://www.reactionbiology.com/wp-content/uploads/2023/07/191220_Mehdi_HAT_assay_principle.png 2560w, https://www.reactionbiology.com/wp-content/uploads/2023/07/191220_Mehdi_HAT_assay_principle-300x40.png 300w, https://www.reactionbiology.com/wp-content/uploads/2023/07/191220_Mehdi_HAT_assay_principle-1024x135.png 1024w, https://www.reactionbiology.com/wp-content/uploads/2023/07/191220_Mehdi_HAT_assay_principle-768x101.png 768w, https://www.reactionbiology.com/wp-content/uploads/2023/07/191220_Mehdi_HAT_assay_principle-1536x202.png 1536w, https://www.reactionbiology.com/wp-content/uploads/2023/07/191220_Mehdi_HAT_assay_principle-2048x270.png 2048w, https://www.reactionbiology.com/wp-content/uploads/2023/07/191220_Mehdi_HAT_assay_principle-1080x142.png 1080w" sizes="(max-width: 5115px) 100vw, 5115px" /><br /> Histone acetyltransferases acetylate lysines on histones and other proteins using tritium-labeled acetyl-Coenzyme A as the acetyl donor. The tritium-acetyl group is transferred onto the histone substrate that is measured directly to reflect the enzyme activity.</div> </div></div></div></div><div id="tab-haah-id222" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Assay setup </div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><strong>Setups</strong>: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.</p> <p><strong>Controls</strong>: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format.</p> <p><strong>Turnaround time</strong>: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.</p> <p><strong>Report</strong>: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.</p> <p><strong>Screening facility</strong>: This assay is performed at our screening facility in Malvern, PA, US.</p> <p><strong>Compound requirements</strong>: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our <a href="/faq/">FAQs</a> for information regarding compound preparation and shipping.</p> <div class="field field--name-field-pdf field--type-file field--label-hidden field__items"> <div class="field__item"><span class="file file--mime-application-vnd-openxmlformats-officedocument-spreadsheetml-sheet file--x-office-spreadsheet"> <a title="FAQ Assay Condition Form" href="https://www.reactionbiology.com/faq/" target="_blank" rel="noopener">Please see our FAQ page to access our assay condition form (US facility).</a></span></div> </div> </div></div></div></div></div></div></div></div><div id="haah-id3" class="paragraph paragraph--type--related-resources-container paragraph--view-mode--default"><div class="container center"><h2>Related Resources</h2><div class="field--name-field-paragraph related-resources-reference-wrapper"><div class="paragraph--type--related-resource node--type-related-resource"><div class="related-resource-wrapper"><div class="field--name-field-image"><div class="field field--name-field-image field--type-image field--label-hidden field__items"><img src="https://www.reactionbiology.com/wp-content/uploads/2023/06/Advertorial-unlocking-the-door.png" alt="Advertorial - Unlocking the Door to Epigenetic Medicine " title="Advertorial - Unlocking the Door to Epigenetic Medicine " typeof="foaf:Image" class="image-style-large"></div></div><div class="related-resource-text"> <div class="field--name-field-resource-category"> Publication </div> <div class="field--name-field-title"> <span class="field field--name-title field--type-string field--label-hidden"> Advertorial - Unlocking the Door to Epigenetic Medicine </span> </div> <div class="field--name-field-download"> <span class="pdfpreview-image-wrapper"> <a href="https://www.reactionbiology.com/wp-content/uploads/2023/06/Advertorial-unlocking-the-door.pdf" target="_blank">Download PDF</a> </span> </div> </div></div></div><div class="paragraph--type--related-resource node--type-related-resource"><div class="related-resource-wrapper"><div class="field--name-field-image"><div class="field field--name-field-image field--type-image field--label-hidden field__items"><img src="https://www.reactionbiology.com/wp-content/uploads/2023/07/801-Poster_Histone-Modification.png" alt="Histone Modification; Reaction Biology Proteins for Epigenetic Research " title="Histone Modification; Reaction Biology Proteins for Epigenetic Research " typeof="foaf:Image" class="image-style-large"></div></div><div class="related-resource-text"> <div class="field--name-field-resource-category"> Poster </div> <div class="field--name-field-title"> <span class="field field--name-title field--type-string field--label-hidden"> Histone Modification; Reaction Biology Proteins for Epigenetic Research </span> </div> <div class="field--name-field-download"> <span class="pdfpreview-image-wrapper"> <a href="https://www.reactionbiology.com/wp-content/uploads/2023/07/Poster_Histone-Modification.pdf" target="_blank">Download PDF</a> </span> </div> </div></div></div><div class="paragraph--type--related-resource node--type-related-resource"><div class="related-resource-wrapper"><div class="field--name-field-image"><div class="field field--name-field-image field--type-image field--label-hidden field__items"><img src="https://www.reactionbiology.com/wp-content/uploads/2023/07/2017_Dahlin_HAT_NatCom_thumb.png" alt="Assay interference and off-target liabilities of reported histone acetyltransferase inhibitors. 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