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models</title> <meta name="description" content="Search results for: in vitro models"> <meta name="keywords" content="in vitro models"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="in vitro models"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 8056</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: in vitro models</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8056</span> Comparison of Different in vitro Models of the Blood-Brain Barrier for Study of Toxic Effects of Engineered Nanoparticles</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Samir%20Dekali">Samir Dekali</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20Crouzier"> David Crouzier</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Due to their new physico-chemical properties engineered nanoparticles (ENPs) are increasingly employed in numerous industrial sectors (such as electronics, textile, aerospace, cosmetics, pharmaceuticals, food industry, etc). These new physico-chemical properties can also represent a threat for the human health. Consumers can notably be exposed involuntarily by different routes such as inhalation, ingestion or through the skin. Several studies recently reported a possible biodistribution of these ENPs on the blood-brain barrier (BBB). Consequently, there is a great need for developing BBB in vitro models representative of the in vivo situation and capable of rapidly and accurately assessing ENPs toxic effects and their potential translocation through this barrier. In this study, several in vitro models established with micro-endothelial brain cell lines of different origins (bEnd.3 mouse cell line or a new human cell line) co-cultivated or not with astrocytic cells (C6 rat or C8-B4 mouse cell lines) on Transwells® were compared using different endpoints: trans-endothelial resistance, permeability of the Lucifer yellow and protein junction labeling. Impact of NIST diesel exhaust particles on BBB cell viability is also discussed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title="nanoparticles">nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=blood-brain%20barrier" title=" blood-brain barrier"> blood-brain barrier</a>, <a href="https://publications.waset.org/abstracts/search?q=diesel%20exhaust%20particles" title=" diesel exhaust particles"> diesel exhaust particles</a>, <a href="https://publications.waset.org/abstracts/search?q=toxicology" title=" toxicology"> toxicology</a> </p> <a href="https://publications.waset.org/abstracts/18338/comparison-of-different-in-vitro-models-of-the-blood-brain-barrier-for-study-of-toxic-effects-of-engineered-nanoparticles" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/18338.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">440</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8055</span> A High Content Screening Platform for the Accurate Prediction of Nephrotoxicity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sijing%20Xiong">Sijing Xiong</a>, <a href="https://publications.waset.org/abstracts/search?q=Ran%20Su"> Ran Su</a>, <a href="https://publications.waset.org/abstracts/search?q=Lit-Hsin%20Loo"> Lit-Hsin Loo</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniele%20Zink"> Daniele Zink</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The kidney is a major target for toxic effects of drugs, industrial and environmental chemicals and other compounds. Typically, nephrotoxicity is detected late during drug development, and regulatory animal models could not solve this problem. Validated or accepted in silico or in vitro methods for the prediction of nephrotoxicity are not available. We have established the first and currently only pre-validated in vitro models for the accurate prediction of nephrotoxicity in humans and the first predictive platforms based on renal cells derived from human pluripotent stem cells. In order to further improve the efficiency of our predictive models, we recently developed a high content screening (HCS) platform. This platform employed automated imaging in combination with automated quantitative phenotypic profiling and machine learning methods. 129 image-based phenotypic features were analyzed with respect to their predictive performance in combination with 44 compounds with different chemical structures that included drugs, environmental and industrial chemicals and herbal and fungal compounds. The nephrotoxicity of these compounds in humans is well characterized. A combination of chromatin and cytoskeletal features resulted in high predictivity with respect to nephrotoxicity in humans. Test balanced accuracies of 82% or 89% were obtained with human primary or immortalized renal proximal tubular cells, respectively. Furthermore, our results revealed that a DNA damage response is commonly induced by different PTC-toxicants with diverse chemical structures and injury mechanisms. Together, the results show that the automated HCS platform allows efficient and accurate nephrotoxicity prediction for compounds with diverse chemical structures. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=high%20content%20screening" title="high content screening">high content screening</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20models" title=" in vitro models"> in vitro models</a>, <a href="https://publications.waset.org/abstracts/search?q=nephrotoxicity" title=" nephrotoxicity"> nephrotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=toxicity%20prediction" title=" toxicity prediction"> toxicity prediction</a> </p> <a href="https://publications.waset.org/abstracts/42832/a-high-content-screening-platform-for-the-accurate-prediction-of-nephrotoxicity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42832.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">312</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8054</span> Factors Affecting the Results of in vitro Gas Production Technique</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=O.%20Kahraman">O. Kahraman</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20S.%20Alatas"> M. S. Alatas</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20B.%20Citil"> O. B. Citil</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In determination of values of feeds which, are used in ruminant nutrition, different methods are used like in vivo, in vitro, in situ or in sacco. Generally, the most reliable results are taken from the in vivo studies. But because of the disadvantages like being hard, laborious and expensive, time consuming, being hard to keep the experiment conditions under control and too much samples are needed, the in vitro techniques are more preferred. The most widely used in vitro techniques are two-staged digestion technique and gas production technique. In vitro gas production technique is based on the measurement of the CO2 which is released as a result of microbial fermentation of the feeds. In this review, the factors affecting the results obtained from in vitro gas production technique (Hohenheim Feed Test) were discussed. Some factors must be taken into consideration when interpreting the findings obtained in these studies and also comparing the findings reported by different researchers for the same feeds. These factors were discussed in 3 groups: factors related to animal, factors related to feeds and factors related with differences in the application of method. These factors and their effects on the results were explained. Also it can be concluded that the use of in vitro gas production technique in feed evaluation routinely can be contributed to the comprehensive feed evaluation, but standardization is needed in this technique to attain more reliable results. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=In%20vitro" title="In vitro">In vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20production%20technique" title=" gas production technique"> gas production technique</a>, <a href="https://publications.waset.org/abstracts/search?q=Hohenheim%20feed%20test" title=" Hohenheim feed test"> Hohenheim feed test</a>, <a href="https://publications.waset.org/abstracts/search?q=standardization" title=" standardization"> standardization</a> </p> <a href="https://publications.waset.org/abstracts/26010/factors-affecting-the-results-of-in-vitro-gas-production-technique" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26010.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">599</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8053</span> Combined Proteomic and Metabolomic Analysis Approaches to Investigate the Modification in the Proteome and Metabolome of in vitro Models Treated with Gold Nanoparticles (AuNPs)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=H.%20Chassaigne">H. Chassaigne</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Gioria"> S. Gioria</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Lobo%20Vicente"> J. Lobo Vicente</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Carpi"> D. Carpi</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Barboro"> P. Barboro</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Tomasi"> G. Tomasi</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Kinsner-Ovaskainen"> A. Kinsner-Ovaskainen</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Rossi"> F. Rossi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Emerging approaches in the area of exposure to nanomaterials and assessment of human health effects combine the use of in vitro systems and analytical techniques to study the perturbation of the proteome and/or the metabolome. We investigated the modification in the cytoplasmic compartment of the Balb/3T3 cell line exposed to gold nanoparticles. On one hand, the proteomic approach is quite standardized even if it requires precautions when dealing with in vitro systems. On the other hand, metabolomic analysis is challenging due to the chemical diversity of cellular metabolites that complicate data elaboration and interpretation. Differentially expressed proteins were found to cover a range of functions including stress response, cell metabolism, cell growth and cytoskeleton organization. In addition, de-regulated metabolites were annotated using the HMDB database. The "omics" fields hold huge promises in the interaction of nanoparticles with biological systems. The combination of proteomics and metabolomics data is possible however challenging. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=data%20processing" title="data processing">data processing</a>, <a href="https://publications.waset.org/abstracts/search?q=gold%20nanoparticles" title=" gold nanoparticles"> gold nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20systems" title=" in vitro systems"> in vitro systems</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolomics" title=" metabolomics"> metabolomics</a>, <a href="https://publications.waset.org/abstracts/search?q=proteomics" title=" proteomics"> proteomics</a> </p> <a href="https://publications.waset.org/abstracts/5961/combined-proteomic-and-metabolomic-analysis-approaches-to-investigate-the-modification-in-the-proteome-and-metabolome-of-in-vitro-models-treated-with-gold-nanoparticles-aunps" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/5961.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">503</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8052</span> Diagonal Vector Autoregressive Models and Their Properties</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Usoro%20Anthony%20E.">Usoro Anthony E.</a>, <a href="https://publications.waset.org/abstracts/search?q=Udoh%20Emediong"> Udoh Emediong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diagonal Vector Autoregressive Models are special classes of the general vector autoregressive models identified under certain conditions, where parameters are restricted to the diagonal elements in the coefficient matrices. Variance, autocovariance, and autocorrelation properties of the upper and lower diagonal VAR models are derived. The new set of VAR models is verified with empirical data and is found to perform favourably with the general VAR models. The advantage of the diagonal models over the existing models is that the new models are parsimonious, given the reduction in the interactive coefficients of the general VAR models. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=VAR%20models" title="VAR models">VAR models</a>, <a href="https://publications.waset.org/abstracts/search?q=diagonal%20VAR%20models" title=" diagonal VAR models"> diagonal VAR models</a>, <a href="https://publications.waset.org/abstracts/search?q=variance" title=" variance"> variance</a>, <a href="https://publications.waset.org/abstracts/search?q=autocovariance" title=" autocovariance"> autocovariance</a>, <a href="https://publications.waset.org/abstracts/search?q=autocorrelations" title=" autocorrelations"> autocorrelations</a> </p> <a href="https://publications.waset.org/abstracts/157980/diagonal-vector-autoregressive-models-and-their-properties" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/157980.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">116</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8051</span> Feed Value of Selected Nigerian Browse Plants: Chemical Composition and in vitro Digestibility</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Isaac%20Samuel">Isaac Samuel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A study was conducted to determine the in-vitro degradation of selected Nigerian browse plants consumed by small ruminants on free range in northern guinea savannah region of Nigeria using in vitro gas production, proximate composition, fibre components, methane gas production and dry matter degradation as tools. The leaves samples of the selected browse plants were collected, processed and incubated using in vitro gas dry matter degradation techniques. Results obtained showed variation in the rate of degradation. The result obtained from chemical analysis showed that the CP content of A. occidentale (26.49%) was higher than F. thonningi (23.58%), M. indica (20.58%) and T. catappa (18.61%). Both ADF and NDF of A. occidentale (40.00 and 50.00) were as well higher than F. thonningi (20.00 and 40.00), M. indica (20.00 and 40.00) and T.catappa (20.00 and 42.00). Results from in vitro gas production however showed that T. catappa (23.67ml/DM) has a significantly higher (p<0.05) value than F.thonningi (20.67ml/DM), A. occidentale (16.67ml/DM), and M. indica(14.00ml/DM) at 72 hours of incubation. Methane gas production and in vitro gas production can be used to predict dry matter degradation and nutritive value of feedstuff for small ruminants. A. occidentale with the least methane gas production and highest crude protein (CP) content might have the most nutritive value among the browse plants investigated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title="in vitro">in vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=degradation" title=" degradation"> degradation</a>, <a href="https://publications.waset.org/abstracts/search?q=browse" title=" browse"> browse</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20production" title=" gas production"> gas production</a> </p> <a href="https://publications.waset.org/abstracts/44408/feed-value-of-selected-nigerian-browse-plants-chemical-composition-and-in-vitro-digestibility" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44408.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">357</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8050</span> Antidiabetic Potential of Pseuduvaria monticola Bark Extract on the Pancreatic Cells, NIT-1 and Type 2 Diabetic Rat Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hairin%20Taha">Hairin Taha</a>, <a href="https://publications.waset.org/abstracts/search?q=Aditya%20Arya"> Aditya Arya</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20A.%20Hapipah"> M. A. Hapipah</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20M.%20Mustafa"> A. M. Mustafa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plants have been an important source of medicine since ancient times. Pseuduvaria monticola is a rare montane forest species from the Annonaceae family. Traditionally, the plant was used to cure symptoms of fever, inflammation, stomach-ache and also to reduce the elevated levels of blood glucose. Scientifically, we have evaluated the antidiabetic potential of the Pseuduvaria monticola bark methanolic extract on certain in vitro cell based assays, followed by in vivo study. Results from in vitro models displayed PMm upregulated glucose uptake and insulin secretion in mouse pancreatic β-cells. In vivo study demonstrated the PMm down-regulated hyperglycaemia, oxidative stress and elevated levels of pro-inflammatory cytokines in type 2 diabetic rat models. Altogether, the study revealed that Pseuduvaria monticola might be used as a potential candidate for the management of type 2 diabetes and its related complications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=type%202%20diabetes" title="type 2 diabetes">type 2 diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=Pseuduvaria%20monticola" title=" Pseuduvaria monticola"> Pseuduvaria monticola</a>, <a href="https://publications.waset.org/abstracts/search?q=insulin%20secretion" title=" insulin secretion"> insulin secretion</a>, <a href="https://publications.waset.org/abstracts/search?q=glucose%20uptake" title=" glucose uptake"> glucose uptake</a> </p> <a href="https://publications.waset.org/abstracts/13005/antidiabetic-potential-of-pseuduvaria-monticola-bark-extract-on-the-pancreatic-cells-nit-1-and-type-2-diabetic-rat-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13005.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">439</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8049</span> Investigating the Successes of in vitro Embryogenesis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zelikha%20Labbani">Zelikha Labbani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The in vitro isolated microspore culture is the most powerful androgenic pathway to produce doubled haploid plants in the short time. To deviate a microspore toward embryogenesis, a number of factors, different for each species, must concur at the same time and place. Once induced, the microspore undergoes numerous changes at different levels, from overall morphology to gene expression. Induction of microspore embryogenesis not only implies the expression of an embryogenic program, but also a stress-related cellular response and a repression of the gametophytic program to revert the microspore to a totipotent status. As haploid single cells, microspore became a strategy to achieve various objectives particularly in genetic engineering. In this communication we would show the most recent advances in the producing haploid embryos via in vitro isolated microspore culture. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20isolated%20microspore%20culture" title="in vitro isolated microspore culture">in vitro isolated microspore culture</a>, <a href="https://publications.waset.org/abstracts/search?q=success" title=" success"> success</a>, <a href="https://publications.waset.org/abstracts/search?q=haploid%20cells" title=" haploid cells"> haploid cells</a>, <a href="https://publications.waset.org/abstracts/search?q=bioinformatics" title=" bioinformatics"> bioinformatics</a>, <a href="https://publications.waset.org/abstracts/search?q=biomedicine" title=" biomedicine"> biomedicine</a> </p> <a href="https://publications.waset.org/abstracts/9122/investigating-the-successes-of-in-vitro-embryogenesis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/9122.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">475</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8048</span> Design, Synthesis and in-vitro Antitumor Evaluation of Some Novel Substituted Quinazoline Derivatives</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adel%20S.%20El-Azab">Adel S. El-Azab</a>, <a href="https://publications.waset.org/abstracts/search?q=Alaa%20A.%20M.%20Abdel-Aziz"> Alaa A. M. Abdel-Aziz</a>, <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20A.%20Al-Suwaidan"> Ibrahim A. Al-Suwaidan</a>, <a href="https://publications.waset.org/abstracts/search?q=Amer%20M.%20Alanazi"> Amer M. Alanazi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A novel series of 2,3,6-trisubstitute quinazolinone were designed, synthesized, and evaluated for their in-vitro antitumor activity. 3 (Benzylideneamino)-6-chloro-2-p-tolylquinazolin-4(3H)-One, 2-[(4-oxo-3-phenethyl-3,4-dihydroquinazolin-2-yl)thio]-N-(3,4;5-trimethoxyphenyl) acetamide and 3-(3-benzyl-6-methyl-4-oxo-3, 4-dihydroquinazolin-2-ylthio)-N-(3,4,5-trimethoxyphenyl) propanamide have shown amazing broad spectrum antitumor activity with mean GI50; 15.8, 3.16, and 7.4 μM respectively compared to known Quinazoline Derivatives antitumor drug 5-FU mean GI50=22.6 μM. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=quinazoline%20derivatives" title="quinazoline derivatives">quinazoline derivatives</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20antitumor" title=" in vitro antitumor"> in vitro antitumor</a>, <a href="https://publications.waset.org/abstracts/search?q=synthesis" title=" synthesis"> synthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=5-FU" title=" 5-FU"> 5-FU</a>, <a href="https://publications.waset.org/abstracts/search?q=NCI" title=" NCI"> NCI</a> </p> <a href="https://publications.waset.org/abstracts/22501/design-synthesis-and-in-vitro-antitumor-evaluation-of-some-novel-substituted-quinazoline-derivatives" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22501.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">544</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8047</span> Effect of IGF-I on Ovine Oocytes Maturation and Subsequent Embryo Development following in Vitro Fertilization (IVF)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Babak%20Qasemi-Panahi">Babak Qasemi-Panahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Gholamali%20Moghaddam"> Gholamali Moghaddam</a>, <a href="https://publications.waset.org/abstracts/search?q=Seyed-Abbas%20Rafat"> Seyed-Abbas Rafat</a>, <a href="https://publications.waset.org/abstracts/search?q=Hossein%20Daghigh%20Kia"> Hossein Daghigh Kia</a>, <a href="https://publications.waset.org/abstracts/search?q=Mansoureh%20Movahedin"> Mansoureh Movahedin</a>, <a href="https://publications.waset.org/abstracts/search?q=Reza%20Hadavi"> Reza Hadavi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The objective of this study was to determine the effects of IGF-I on ovine oocytes maturation and subsequent development of embryos derived from in vitro fertilization (IVF). In vitro maturation (IVM) of oocytes and in vitro culture (IVC) of embryos was conducted with or without 100 ng/mL IGF-1. In the IGF-I treated group, mean percentage of oocyte maturation was significantly higher than the control group (57.67 ± 3.04 versus 49.81 ± 3.04%, respectively, P < 0.05). However, in comparison with control group, there was no significant effect of IGF-1 on rates of cleavage, morula, and blastocyst formation (85% versus 84%; 63% versus 65%, and 40% to 39%, respectively). These data demonstrate that IGF-I has a positive effect on ovine oocyte maturation rate, but it has not the significant outcome on embryo development. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ovine" title="ovine">ovine</a>, <a href="https://publications.waset.org/abstracts/search?q=IGF-I" title=" IGF-I"> IGF-I</a>, <a href="https://publications.waset.org/abstracts/search?q=IVM" title=" IVM"> IVM</a>, <a href="https://publications.waset.org/abstracts/search?q=ICSI" title=" ICSI"> ICSI</a> </p> <a href="https://publications.waset.org/abstracts/21011/effect-of-igf-i-on-ovine-oocytes-maturation-and-subsequent-embryo-development-following-in-vitro-fertilization-ivf" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21011.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">688</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8046</span> Successes on in vitro Isolated Microspores Embryogenesis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zelikha%20Labbani">Zelikha Labbani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The In Vitro isolated micro spore culture is the most powerful androgenic pathway to produce doubled haploid plants in the short time. To deviate a micro spore toward embryogenesis, a number of factors, different for each species, must concur at the same time and place. Once induced, the micro spore undergoes numerous changes at different levels, from overall morphology to gene expression. Induction of micro spore embryogenesis not only implies the expression of an embryogenic program, but also a stress-related cellular response and a repression of the gametophytic program to revert the microspore to a totipotent status. As haploid single cells, micro spore became a strategy to achieve various objectives particularly in genetic engineering. In this study we would show the most recent advances in the producing haploid embryos via In Vitro isolated micro spore culture. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=haploid%20cells" title="haploid cells">haploid cells</a>, <a href="https://publications.waset.org/abstracts/search?q=In%20Vitro%20isolated%20microspore%20culture" title=" In Vitro isolated microspore culture"> In Vitro isolated microspore culture</a>, <a href="https://publications.waset.org/abstracts/search?q=success" title=" success"> success</a> </p> <a href="https://publications.waset.org/abstracts/26693/successes-on-in-vitro-isolated-microspores-embryogenesis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26693.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">615</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8045</span> A Sub-Conjunctiva Injection of Rosiglitazone for Anti-Fibrosis Treatment after Glaucoma Filtration Surgery</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yang%20Zhao">Yang Zhao</a>, <a href="https://publications.waset.org/abstracts/search?q=Feng%20Zhang"> Feng Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xuanchu%20Duan"> Xuanchu Duan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Trans-differentiation of human Tenon fibroblasts (HTFs) to myo-fibroblasts and fibrosis of episcleral tissue are the most common reasons for the failure of glaucoma filtration surgery, with limited treatment options like antimetabolites which always have side-effects such as leakage of filter bulb, infection, hypotony, and endophthalmitis. Rosiglitazone, a specific thiazolidinedione is a synthetic high-affinity ligand for PPAR-r, which has been used in the treatment of type2 diabetes, and found to have pleiotropic functions against inflammatory response, cell proliferation and tissue fibrosis and to benefit to a variety of diseases in animal myocardium models, steatohepatitis models, etc. Here, in vitro we cultured primary HTFs and stimulated with TGF- β to induced myofibrogenic, then treated cells with Rosiglitazone to assess for fibrogenic response. In vivo, we used rabbit glaucoma model to establish the formation of post- trabeculectomy scarring. Then we administered subconjunctival injection with Rosiglitazone beside the ﬁltering bleb, later protein, mRNA and immunofluorescence of fibrogenic markers are checked, and filtering bleb condition was measured. In vitro, we found Rosiglitazone could suppressed proliferation and migration of fibroblasts through macroautophagy via TGF- β /Smad signaling pathway. In vivo, on postoperative day 28, the mean number of ﬁbroblasts in Rosiglitazone injection group was signiﬁcantly the lowest and had the least collagen content and connective tissue growth factor. Rosiglitazone effectively controlled human and rabbit fibroblasts in vivo and in vitro. Its subconjunctiiva application may represent an effective, new avenue for the prevention of scarring after glaucoma surgery. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fibrosis" title="fibrosis">fibrosis</a>, <a href="https://publications.waset.org/abstracts/search?q=glaucoma" title=" glaucoma"> glaucoma</a>, <a href="https://publications.waset.org/abstracts/search?q=macroautophagy" title=" macroautophagy"> macroautophagy</a>, <a href="https://publications.waset.org/abstracts/search?q=rosiglitazone" title=" rosiglitazone"> rosiglitazone</a> </p> <a href="https://publications.waset.org/abstracts/73539/a-sub-conjunctiva-injection-of-rosiglitazone-for-anti-fibrosis-treatment-after-glaucoma-filtration-surgery" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/73539.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">274</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8044</span> On In vitro Durum Wheat Isolated Microspore Culture</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zelikha%20Labbani">Zelikha Labbani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Since its creation in 1964 by Guha and Maheshwari in India on Datura innoxia Mill, in vitro androgenesis has become the method of choice in the production of doubled haploid in many species. However, in durum wheat, the Doubled haploid plant breeding programs remained limited due to the low production of androgenetic embryos and converting them into fertile green plants. We describe here an efficient method for inducing embryos and regenerating green plants directly from isolated microspores of durum wheat. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=durum%20wheat" title="durum wheat">durum wheat</a>, <a href="https://publications.waset.org/abstracts/search?q=haploid%20embryos" title=" haploid embryos"> haploid embryos</a>, <a href="https://publications.waset.org/abstracts/search?q=on%20in%20vitro" title=" on in vitro"> on in vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=pretreatment" title=" pretreatment"> pretreatment</a> </p> <a href="https://publications.waset.org/abstracts/44239/on-in-vitro-durum-wheat-isolated-microspore-culture" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44239.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">355</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8043</span> A Comparative Evaluation of Antioxidant Activity of in vivo and in vitro Raised Holarrhena antidysenterica Linn.</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gayatri%20Nahak">Gayatri Nahak</a>, <a href="https://publications.waset.org/abstracts/search?q=Satyajit%20Kanungo"> Satyajit Kanungo</a>, <a href="https://publications.waset.org/abstracts/search?q=Rajani%20Kanta%20Sahu"> Rajani Kanta Sahu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Holarrhena antidysenterica Linn. (Apocynaceae) is a typical Indian medicinal plant popularly known as “Indrajav”. Traditionally the plant has been considered a popular remedy for the treatment of dysentery, diarrhea, intestinal worms and the seeds of this plant are also used as an anti-diabetic remedy. In the present study axillary shoot multiplication, callus induction and shoot regeneration from callus culture were obtained on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators. Then in vivo and in vitro grown healthy plants were selected for study of antioxidant activity through DPPH and OH methods. Significantly higher antioxidant activity and phenol contents were observed in vitro raised plant in comparison to in vivo plants. The findings indicated the greater amount of phenolic compounds leads to more potent radical scavenging effect as shown in in vitro raised plant in comparison to in vivo plants which showed the ability to utilize tissue culture techniques towards development of desired bioactive metabolites from in vitro culture as an alternative way to avoid using endangered plants in pharmaceutical purposes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Holarrhena%20antidysenterica" title="Holarrhena antidysenterica">Holarrhena antidysenterica</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vivo" title=" in vivo"> in vivo</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a> </p> <a href="https://publications.waset.org/abstracts/16353/a-comparative-evaluation-of-antioxidant-activity-of-in-vivo-and-in-vitro-raised-holarrhena-antidysenterica-linn" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16353.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">510</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8042</span> 3D Microscopy, Image Processing, and Analysis of Lymphangiogenesis in Biological Models</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Thomas%20Louis">Thomas Louis</a>, <a href="https://publications.waset.org/abstracts/search?q=Irina%20Primac"> Irina Primac</a>, <a href="https://publications.waset.org/abstracts/search?q=Florent%20Morfoisse"> Florent Morfoisse</a>, <a href="https://publications.waset.org/abstracts/search?q=Tania%20Durre"> Tania Durre</a>, <a href="https://publications.waset.org/abstracts/search?q=Silvia%20Blacher"> Silvia Blacher</a>, <a href="https://publications.waset.org/abstracts/search?q=Agnes%20Noel"> Agnes Noel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In vitro and in vivo lymphangiogenesis assays are essential for the identification of potential lymphangiogenic agents and the screening of pharmacological inhibitors. In the present study, we analyse three biological models: in vitro lymphatic endothelial cell spheroids, in vivo ear sponge assay, and in vivo lymph node colonisation by tumour cells. These assays provide suitable 3D models to test pro- and anti-lymphangiogenic factors or drugs. 3D images were acquired by confocal laser scanning and light sheet fluorescence microscopy. Virtual scan microscopy followed by 3D reconstruction by image aligning methods was also used to obtain 3D images of whole large sponge and ganglion samples. 3D reconstruction, image segmentation, skeletonisation, and other image processing algorithms are described. Fixed and time-lapse imaging techniques are used to analyse lymphatic endothelial cell spheroids behaviour. The study of cell spatial distribution in spheroid models enables to detect interactions between cells and to identify invasion hierarchy and guidance patterns. Global measurements such as volume, length, and density of lymphatic vessels are measured in both in vivo models. Branching density and tortuosity evaluation are also proposed to determine structure complexity. Those properties combined with vessel spatial distribution are evaluated in order to determine lymphangiogenesis extent. Lymphatic endothelial cell invasion and lymphangiogenesis were evaluated under various experimental conditions. The comparison of these conditions enables to identify lymphangiogenic agents and to better comprehend their roles in the lymphangiogenesis process. The proposed methodology is validated by its application on the three presented models. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=3D%20image%20segmentation" title="3D image segmentation">3D image segmentation</a>, <a href="https://publications.waset.org/abstracts/search?q=3D%20image%20skeletonisation" title=" 3D image skeletonisation"> 3D image skeletonisation</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20invasion" title=" cell invasion"> cell invasion</a>, <a href="https://publications.waset.org/abstracts/search?q=confocal%20microscopy" title=" confocal microscopy"> confocal microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=ear%20sponges" title=" ear sponges"> ear sponges</a>, <a href="https://publications.waset.org/abstracts/search?q=light%20sheet%20microscopy" title=" light sheet microscopy"> light sheet microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=lymph%20nodes" title=" lymph nodes"> lymph nodes</a>, <a href="https://publications.waset.org/abstracts/search?q=lymphangiogenesis" title=" lymphangiogenesis"> lymphangiogenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=spheroids" title=" spheroids"> spheroids</a> </p> <a href="https://publications.waset.org/abstracts/87470/3d-microscopy-image-processing-and-analysis-of-lymphangiogenesis-in-biological-models" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/87470.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">377</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8041</span> Effects of Fenugreek Seed Extract on in vitro Maturation and Subsequent Development of Sheep Oocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20A.%20H.%20Barakat">Ibrahim A. H. Barakat</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20R.%20Al-Himaidi"> Ahmed R. Al-Himaidi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present study was conducted to determine the role and optimum concentration of fenugreek seed extract during in-vitro maturation on in-vitro maturation and developmental competence of Neaimi sheep oocytes following in-vitro fertilization. The Cumulus Oocyte Complexes (COCs) collected from sheep slaughterhouse ovaries were randomly divided into three groups, and they were matured for 24 hrs. in maturation medium containing fenugreek seed extract (0, 1 and 10 µg ml-1). Oocytes of a control group were matured in a medium containing 1 µg ml-1 estradiol 17β. After maturation, half of oocytes were fixed and stained for evaluation of nuclear maturation. The rest of oocytes were fertilized in vitro with fresh semen, then cultured for 9 days for the assessment of the developmental capacity of the oocytes. The results showed that the mean values of oocytes with expanded cumulus cells percentage were not significantly different among all groups (P < 0.05). But nuclear maturation rate of oocytes matured with 10 µg ml-1 fenugreek seed extract was significantly higher than that of the control group. The maturation rate and development to morula and blastocyst stage for oocytes matured at 10 µg ml-1 fenugreek seed extract was significantly higher than those matured at 1µg ml-1 of fenugreek seed extract and the control group. In conclusion, better maturation and developmental capacity rate to morula and blastocyst stage were obtained by the addition of 10 µg ml-1 fenugreek seed extract to maturation medium than addition of 1 µg ml-1 estradiol-17β (P < 0.05). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fenugreek%20seed%20extract" title="fenugreek seed extract">fenugreek seed extract</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20maturation" title=" in vitro maturation"> in vitro maturation</a>, <a href="https://publications.waset.org/abstracts/search?q=sheep%20oocytes" title=" sheep oocytes"> sheep oocytes</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20fertilization" title=" in vitro fertilization"> in vitro fertilization</a>, <a href="https://publications.waset.org/abstracts/search?q=embryo%20development" title=" embryo development"> embryo development</a> </p> <a href="https://publications.waset.org/abstracts/3185/effects-of-fenugreek-seed-extract-on-in-vitro-maturation-and-subsequent-development-of-sheep-oocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3185.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">392</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8040</span> Development of an in vitro Fermentation Chicken Ileum Microbiota Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bello%20Gonzalez">Bello Gonzalez</a>, <a href="https://publications.waset.org/abstracts/search?q=Setten%20Van%20M."> Setten Van M.</a>, <a href="https://publications.waset.org/abstracts/search?q=Brouwer%20M."> Brouwer M.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The chicken small intestine represents a dynamic and complex organ in which the enzymatic digestion and absorption of nutrients take place. The development of an in vitro fermentation chicken small intestinal model could be used as an alternative to explore the interaction between the microbiota and nutrient metabolism and to enhance the efficacy of targeting interventions to improve animal health. In the present study we have developed an in vitro fermentation chicken ileum microbiota model for unrevealing the complex interaction of ileum microbial community under physiological conditions. A two-vessel continuous fermentation process simulating in real-time the physiological conditions of the ileum content (pH, temperature, microaerophilic/anoxic conditions, and peristaltic movements) has been standardized as a proof of concept. As inoculum, we use a pool of ileum microbial community obtained from chicken broilers at the age of day 14. The development and validation of the model provide insight into the initial characterization of the ileum microbial community and its dynamics over time-related to nutrient assimilation and fermentation. Samples can be collected at different time points and can be used to determine the microbial compositional structure, dynamics, and diversity over time. The results of studies using this in vitro model will serve as the foundation for the development of a whole small intestine in vitro fermentation chicken gastrointestinal model to complement our already established in vitro fermentation chicken caeca model. The insight gained from this model could provide us with some information about the nutritional strategies to restore and maintain chicken gut homeostasis. Moreover, the in vitro fermentation model will also allow us to study relationships between gut microbiota composition and its dynamics over time associated with nutrients, antimicrobial compounds, and disease modelling. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=broilers" title="broilers">broilers</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20model" title=" in vitro model"> in vitro model</a>, <a href="https://publications.waset.org/abstracts/search?q=ileum%20microbiota" title=" ileum microbiota"> ileum microbiota</a>, <a href="https://publications.waset.org/abstracts/search?q=fermentation" title=" fermentation"> fermentation</a> </p> <a href="https://publications.waset.org/abstracts/185845/development-of-an-in-vitro-fermentation-chicken-ileum-microbiota-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/185845.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">57</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8039</span> In vitro and invivo Antioxidant Studies of Grewia crenata Leaves Extract in Albino Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20N.Ukwuani">A. N.Ukwuani</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20K.%20Abdulfatah"> A. K. Abdulfatah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> G. crenata is used locally for the treatment of fractured bones, wound healing and inflammatory conditions. In vitro and in vivo antioxidant activity of hydromethanolic extracts of the leaves of G. crenata were assessed. The phytochemical analysis shows the presence of phenols, flavonoids, saponins, cardiac glycosides and tannins. An in vitro quantitative analysis of phenols, flavonoids and tannins respectively were (164±1.20, 199±0.88 and 88.67±0.88 mg/100g FW). In vivo studies of hydromethanolic extract demonstrated a dose dependent increase in hepatic superoxide dismutase (1.14±0.14, 2.13±0.11, 2.55±0.11 U/mg Protein) with improvement in hepatic glutathione (6.98±0.42, 8.91±0.37, 11.07±0.46 µM/mg Protein) and Catalase (4.47±0.05, 6.24±0.02, 7.17±0.04 U/mg Protein) and Total protein (6.18±0.08, 6.69±0.18, 7.27±0.16 mg/ml) respectively at 100-300mg/kg body weight Grewia crenata leaves when compared to the control and standard drug. It can be concluded from the present findings of that G. crenata leaves possess antioxidant potential. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Grewia%20crenata" title="Grewia crenata">Grewia crenata</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=hydromethanolic%20extract" title=" hydromethanolic extract"> hydromethanolic extract</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vivo" title=" in vivo"> in vivo</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a> </p> <a href="https://publications.waset.org/abstracts/15568/in-vitro-and-invivo-antioxidant-studies-of-grewia-crenata-leaves-extract-in-albino-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15568.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">553</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8038</span> The Effects of in vitro Digestion on Cheese Bioactivity; Comparing Adult and Elderly Simulated in vitro Gastrointestinal Digestion Models</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20M.%20Plante">A. M. Plante</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20O%E2%80%99Halloran"> F. O’Halloran</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20L.%20McCarthy"> A. L. McCarthy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> By 2050 it is projected that 2 billion of the global population will be more than 60 years old. Older adults have unique dietary requirements and aging is associated with physiological changes that affect appetite, sensory perception, metabolism, and digestion. Therefore, it is essential that foods recommended and designed for older adults promote healthy aging. To assess cheese as a functional food for the elderly, a range of commercial cheese products were selected and compared for their antioxidant properties. Cheese from various milk sources (bovine, goats, sheep) with different textures and fat content, including cheddar, feta, goats, brie, roquefort, halloumi, wensleydale and gouda, were initially digested with two different simulated in vitro gastrointestinal digestion (SGID) models. One SGID model represented a validated in vitro adult digestion system and the second model, an elderly SGID, was designed to consider the physiological changes associated with aging. The antioxidant potential of all cheese digestates was investigated using in vitro chemical-based antioxidant assays, (2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP) and total phenolic content (TPC)). All adult model digestates had high antioxidant activity across both DPPH ( > 70%) and FRAP ( > 700 µM Fe²⁺/kg.fw) assays. Following in vitro digestion using the elderly SGID model, full-fat red cheddar, low-fat white cheddar, roquefort, halloumi, wensleydale, and gouda digestates had significantly lower (p ≤ 0.05) DPPH radical scavenging properties compared to the adult model digestates. Full-fat white cheddar had higher DPPH radical scavenging activity following elderly SGID digestion compared to the adult model digestate, but the difference was not significant. All other cheese digestates from the elderly model were comparable to the digestates from the adult model in terms of radical scavenging activity. The FRAP of all elderly digestates were significantly lower (p ≤ 0.05) compared to the adult digestates. Goats cheese was significantly higher (p ≤ 0.05) in FRAP (718 µM Fe²/kg.fw) compared to all other digestates in the elderly model. TPC levels in the soft cheeses (feta, goats) and low-fat cheeses (red cheddar, white cheddar) were significantly lower (p ≤ 0.05) in the elderly digestates compared to the adult digestates. There was no significant difference in TPC levels, between the elderly and adult model for full-fat cheddar (red, white), roquefort, wensleydale, gouda, and brie digestates. Halloumi cheese was the only cheese that was significantly higher in TPC levels following elderly digestion compared to adult digestates. Low fat red cheddar had significantly higher (p ≤ 0.05) TPC levels compared to all other digestates for both adult and elderly digestive systems. Findings from this study demonstrate that aging has an impact on the bioactivity of cheese, as antioxidant activity and TPC levels were lower, following in vitro elderly digestion compared to the adult model. For older adults, soft cheese, particularly goats cheese, was associated with high radical scavenging and reducing power, while roquefort cheese had low antioxidant activity. Also, elderly digestates of halloumi and low-fat red cheddar were associated with high TPC levels. Cheese has potential as a functional food for the elderly, however, bioactivity can vary depending on the cheese matrix. Funding for this research was provided by the RISAM Scholarship Scheme, Cork Institute of Technology, Ireland. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidants" title="antioxidants">antioxidants</a>, <a href="https://publications.waset.org/abstracts/search?q=cheese" title=" cheese"> cheese</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro%20digestion" title=" in-vitro digestion"> in-vitro digestion</a>, <a href="https://publications.waset.org/abstracts/search?q=older%20adults" title=" older adults"> older adults</a> </p> <a href="https://publications.waset.org/abstracts/81497/the-effects-of-in-vitro-digestion-on-cheese-bioactivity-comparing-adult-and-elderly-simulated-in-vitro-gastrointestinal-digestion-models" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/81497.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">228</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8037</span> Biofertilization of Cucumber (Cucumis sativus L.) Using Trichoderma longibrachiatum</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kehinde%20T.%20Kareem">Kehinde T. Kareem</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The need to increase the production of cucumber has led to the use of inorganic fertilizers. This chemical affects the ecological balance of nature by increasing the nitrogen and phosphorus contents of the soil. Surface runoffs into rivers and streams cause eutrophication which affects aquatic organisms as well as the consumers of aquatic animals. Therefore, this study was carried out in the screenhouse to investigate the use of a plant growth-promoting fungus; Trichoderma longibrachiatum for the growth promotion of conventional and in-vitro propagated Ashley and Marketmoor cucumber. Before planting of cucumber, spore suspension (108 cfu/ml) of Trichoderma longibrachiatum grown on Potato dextrose agar (PDA) was inoculated into the soil. Fruits were evaluated for the presence of Trichoderma longibrachiatum using a species-specific primer. Results revealed that the highest significant plant height produced by in-vitro propagated Ashley was 19 cm while the highest plant height of in-vitro propagated Marketmoor was 19.67 cm. The yield of the conventional propagated Ashley cucumber showed that the number of fruit/plant obtained from T. longibrachiatum-fertilized plants were significantly more than those of the control. The in-vitro Ashely had 7 fruits/plant while the control produced 4 fruits/plant. In-vitro Marketmoor had ten fruits/plant, and the control had a value of 4 fruits/plant. There were no traces of Trichoderma longibrachiatum genes in the harvested cucumber fruits. Therefore, the use of Trichoderma longibrachiatum as a plant growth-promoter is safe for human health as well as the environment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofertilizer" title="biofertilizer">biofertilizer</a>, <a href="https://publications.waset.org/abstracts/search?q=cucumber" title=" cucumber"> cucumber</a>, <a href="https://publications.waset.org/abstracts/search?q=genes" title=" genes"> genes</a>, <a href="https://publications.waset.org/abstracts/search?q=growth-promoter" title=" growth-promoter"> growth-promoter</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro" title=" in-vitro"> in-vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=propagation" title=" propagation"> propagation</a> </p> <a href="https://publications.waset.org/abstracts/56965/biofertilization-of-cucumber-cucumis-sativus-l-using-trichoderma-longibrachiatum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56965.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">244</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8036</span> Students' Perception of Using Dental E-Models in an Inquiry-Based Curriculum</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yanqi%20Yang">Yanqi Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Chongshan%20Liao"> Chongshan Liao</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheuk%20Hin%20Ho"> Cheuk Hin Ho</a>, <a href="https://publications.waset.org/abstracts/search?q=Susan%20Bridges"> Susan Bridges </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aim: To investigate student’s perceptions of using e-models in an inquiry-based curriculum. Approach: 52 second-year dental students completed a pre- and post-test questionnaire relating to their perceptions of e-models and their use in inquiry-based learning. The pre-test occurred prior to any learning with e-models. The follow-up survey was conducted after one year's experience of using e-models. Results: There was no significant difference between the two sets of questionnaires regarding student’s perceptions of the usefulness of e-models and their willingness to use e-models in future inquiry-based learning. Most of the students preferred using both plaster models and e-models in tandem. Conclusion: Students did not change their attitude towards e-models and most of them agreed or were neutral that e-models are useful in inquiry-based learning. Whilst recognizing the utility of 3D models for learning, student's preference for combining these with solid models has implications for the development of haptic sensibility in an operative discipline. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=e-models" title="e-models">e-models</a>, <a href="https://publications.waset.org/abstracts/search?q=inquiry-based%20curriculum" title=" inquiry-based curriculum"> inquiry-based curriculum</a>, <a href="https://publications.waset.org/abstracts/search?q=education" title=" education"> education</a>, <a href="https://publications.waset.org/abstracts/search?q=questionnaire" title=" questionnaire"> questionnaire</a> </p> <a href="https://publications.waset.org/abstracts/3739/students-perception-of-using-dental-e-models-in-an-inquiry-based-curriculum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3739.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">431</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8035</span> Selection of Developmental Stages of Bovine in vitro-Derived Blastocysts Prior to Vitrification and Embryo Transfer: Implications for Cattle Breeding Programs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Van%20Huong%20Do">Van Huong Do</a>, <a href="https://publications.waset.org/abstracts/search?q=Simon%20Walton"> Simon Walton</a>, <a href="https://publications.waset.org/abstracts/search?q=German%20Amaya"> German Amaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Madeline%20Batsiokis"> Madeline Batsiokis</a>, <a href="https://publications.waset.org/abstracts/search?q=Sally%20Catt"> Sally Catt</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrew%20Taylor-Robinson"> Andrew Taylor-Robinson</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Identification of the most suitable stages of bovine in vitro-derived blastocysts (early, expanded and hatching) prior to vitrification is a straightforward process that facilitates the decision as to which blastocyst stage to use for transfer of fresh and vitrified embryos. Research on in vitro evaluation of suitable stages has shown that the more advanced developmental stage of blastocysts is recommended for fresh embryo transfer while the earlier stage is proposed for embryo transfer following vitrification. There is, however, limited information on blastocyst stages using in vivo assessment. Hence, the aim of the present study was to determine the optimal stage of a blastocyst for vitrification and embryo transfer through a two-step procedure of embryo transfer followed by pregnancy testing at 35, 60 and 90 days of pregnancy. 410 good quality oocytes aspirated by the ovum pick-up technique from 8 donor cows were subjected to in vitro embryo production, vitrification and embryo transfer. Good quality embryos were selected, subjected to vitrification and embryo transfer. Subsequently, 77 vitrified embryos at different blastocyst stages were transferred to synchronised recipient cows. The overall cleavage and blastocyst rates of oocytes were 68.8% and 41.7%, respectively. In addition, the fertility and blastocyst production of 6 bulls used for in vitro fertilization was examined and shown to be statistically different (P<0.05). Results of ongoing pregnancy trials conducted at 35 days, 60 days and 90 days will be discussed. However, preliminary data indicate that individual bulls demonstrate distinctly different fertility performance in vitro. Findings from conception rates would provide a useful tool to aid selection of bovine in vitro-derived embryos for vitrification and embryo transfer in commercial settings. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=blastocyst" title="blastocyst">blastocyst</a>, <a href="https://publications.waset.org/abstracts/search?q=embryo%20transfer" title=" embryo transfer"> embryo transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro-derived%20embryos" title=" in vitro-derived embryos"> in vitro-derived embryos</a>, <a href="https://publications.waset.org/abstracts/search?q=ovum%20pick-up" title=" ovum pick-up"> ovum pick-up</a>, <a href="https://publications.waset.org/abstracts/search?q=vitrification" title=" vitrification"> vitrification</a> </p> <a href="https://publications.waset.org/abstracts/78837/selection-of-developmental-stages-of-bovine-in-vitro-derived-blastocysts-prior-to-vitrification-and-embryo-transfer-implications-for-cattle-breeding-programs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/78837.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">306</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8034</span> The Evaluation of Substitution of Acacia villosa in Ruminants Ration</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hadriana%20Bansi">Hadriana Bansi</a>, <a href="https://publications.waset.org/abstracts/search?q=Elizabeth%20Wina"> Elizabeth Wina</a>, <a href="https://publications.waset.org/abstracts/search?q=Toto%20Toharmat"> Toto Toharmat</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Acacia villosa is thornless shrub legume which contents high crude protein. However, the utilization of A. villosa as ruminant feed is limited by its secondary compounds. The aim of this article is to find out the maximum of substitution A. villosa in sheep ration. The nutritional evaluation consisted of in vitro two stages, in vivo, and in vitro gas production trials. The secondary compounds of A. villosa also were analyzed. Evaluating digestibility of increasing level of substitution A. villosa replacing Pennisetum purpureum was using in vitro two stages. The substitution of 30% A. villosa was compared to 100% P. purpureum by in vitro gas production technique and in vivo digestibility. The results of two stages in vitro showed that total phenol, condensed tannin, and non-protein amino acid (NPAA) were high. Substitution 15% A. villosa reached the highest digestibility for both dry matter (DM) and crude protein (CP) which were 67% and 86% respectively, but it was shown that DM and CP digestibility of substitution 30% of A. villosa was still high which were 61.82% and 75-67% respectively. The pattern of gas production showed that first 8 hours total gas production substitution of 30% A. villosa was higher than 100% P. purpureum and declined after 10 hours incubation. In vivo trials showed that substitution of 30% A. villosa significantly increased CP intake, CP digestibility, and nitrogen retention. It can be concluded that substitution A. villosa until 30% still gave the good impact even though it has high secondary compounds. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acacia%20villosa" title="Acacia villosa">Acacia villosa</a>, <a href="https://publications.waset.org/abstracts/search?q=digestibility" title=" digestibility"> digestibility</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20production" title=" gas production"> gas production</a>, <a href="https://publications.waset.org/abstracts/search?q=secondary%20compounds" title=" secondary compounds"> secondary compounds</a> </p> <a href="https://publications.waset.org/abstracts/96358/the-evaluation-of-substitution-of-acacia-villosa-in-ruminants-ration" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96358.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">163</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8033</span> Antidiabetic and Admet Pharmacokinetic Properties of Grewia Lasiocarpa E. Mey. Ex Harv. Stem Bark Extracts: An in Vitro and in Silico Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Akwu%20N.%20A.">Akwu N. A.</a>, <a href="https://publications.waset.org/abstracts/search?q=Naidoo%20Y."> Naidoo Y.</a>, <a href="https://publications.waset.org/abstracts/search?q=Salau%20V.%20F."> Salau V. F.</a>, <a href="https://publications.waset.org/abstracts/search?q=Olofinsan%20K.%20A."> Olofinsan K. A.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Grewia lasiocarpa E. Mey. ex Harv. (Malvaceae) is a Southern African medicinal plant indigenously used with other plants for birthing problems. The anti-diabetic properties of the hexane, chloroform, and methanol extracts of Grewia lasiocarpa stem bark were assessed using in vitro α-glucosidase enzyme inhibition assay. The predictive in silico drug-likeness and toxicity properties of the phytocompounds were conducted using the pKCSM, ADMElab, and SwissADME computer-aided online tools. The highest α-glucosidase percentage inhibition was observed in the hexane extract (86.76%, IC50= 0.24 mg/mL), followed by chloroform (63.08%, IC50= 4.87 mg/mL) and methanol (53.22%, IC50= 9.41 mg/mL); while acarbose, the standard anti-diabetic drug was (84.54%, IC50= 1.96 mg/mL). The α-glucosidase assay revealed that the hexane extract exhibited the strongest carbohydrate inhibiting capacity and is a better inhibitor than the standard reference drug-acarbose. The computational studies also affirm the results observed in the in vitroα-glucosidaseassay. Thus, the extracts of G. lasiocarpa may be considered a potential plant-sourced compound for treating type 2 diabetes mellitus. This is the first study on the anti-diabetic properties of Grewia lasiocarpa hexane, chloroform, and methanol extracts using in vitro and in silico models. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=grewia%20lasiocarpa" title="grewia lasiocarpa">grewia lasiocarpa</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase%20inhibition" title=" α-glucosidase inhibition"> α-glucosidase inhibition</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-diabetes" title=" anti-diabetes"> anti-diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=ADMET" title=" ADMET"> ADMET</a> </p> <a href="https://publications.waset.org/abstracts/151917/antidiabetic-and-admet-pharmacokinetic-properties-of-grewia-lasiocarpa-e-mey-ex-harv-stem-bark-extracts-an-in-vitro-and-in-silico-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/151917.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">104</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8032</span> In-vitro Antioxidant Activity of Two Selected Herbal Medicines</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Vinotha">S. Vinotha</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Thabrew"> I. Thabrew</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Sri%20Ranjani"> S. Sri Ranjani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hot aqueous and methanol extracts of the two selected herbal medicines such are Vellarugu Chooranam (V.C) and Amukkirai Chooranam (A.C) were examined for total phenolic and flavonoid contents and in-vitro antioxidant activity using four different methods. The total phenolic and flavonoid contents in methanol extract of V.C were found to be higher (44.41±1.26 mg GAE⁄g; 174.44±9.32 mg QE⁄g) than in the methanol extract of A.C (20.56±0.67 mg GAE⁄g;7.21±0.85 mg QE⁄g). Hot methanol and aqueous extracts of both medicines showed low antioxidant activity in DPPH, ABTS, and FRAP methods and Iron chelating activity not found at highest possible concentration. V.C contains higher concentrations of total phenolic and flavonoid contents than A.C and can also exert greater antioxidant activity than A.C, although the activities demonstrated were lower than the positive control Trolox. The in-vitro antioxidant activity was not related with the total phenolic and flavonoid contents of the methanol and aqueous extracts of both herbal medicines (A.C and V.C). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=activity" title="activity">activity</a>, <a href="https://publications.waset.org/abstracts/search?q=different%20extracts" title=" different extracts"> different extracts</a>, <a href="https://publications.waset.org/abstracts/search?q=herbal%20medicines" title=" herbal medicines"> herbal medicines</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro%20antioxidant" title=" in-vitro antioxidant"> in-vitro antioxidant</a> </p> <a href="https://publications.waset.org/abstracts/16823/in-vitro-antioxidant-activity-of-two-selected-herbal-medicines" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16823.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">405</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8031</span> Growth of Albizia in vitro: Endophytic Fungi as Plant Growth Promote of Albizia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reine%20Suci%20Wulandari">Reine Suci Wulandari</a>, <a href="https://publications.waset.org/abstracts/search?q=Rosa%20Suryantini"> Rosa Suryantini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Albizia (Paraserianthes falcataria) is a woody plant species that has a high economic value and multifunctional. Albizia is important timber, medicinal plants and can also be used as a plant to rehabilitate critical lands. The demand value of Albizia is increased so that the large quantities and high quality of seeds are required. In vitro propagation techniques are seed propagation that can produce more seeds and quality in a short time. In vitro cultures require growth regulators that can be obtained from biological agents such as endophytic fungi. Endophytic fungi are micro fungi that colonize live plant tissue without producing symptoms or other negative effects on host plants and increase plant growth. The purposes of this research were to isolate and identify endophytic fungi isolated from the root of Albizia and to study the effect of endophytic fungus on the growth of Albizia in vitro. The methods were root isolation, endophytic fungal identification, and inoculation of endophytic fungi to Albizia plants in vitro. Endophytic fungus isolates were grown on PDA media before being inoculated with Albizia sprouts. Incubation is done for 4 (four) weeks. The observed growth parameters were live explant percentage, percentage of explant shoot, and percentage of explant rooted. The results of the research showed that 6 (six) endophytic fungal isolates obtained from the root of Albizia, namely Aspergillus sp., Verticillium sp, Penicillium sp., Trichoderma sp., Fusarium sp., and Acremonium sp. Statistical analysis found that Trichoderma sp. and Fusarium sp. affect in vitro growth of Albizia. Endophytic fungi from the results of this research were potential as plant growth promoting. It can be applied to increase productivity either through increased plant growth and increased endurance of Albizia seedlings to pests and diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Albizia" title="Albizia">Albizia</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title=" endophytic fungi"> endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=propagation" title=" propagation"> propagation</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a> </p> <a href="https://publications.waset.org/abstracts/74725/growth-of-albizia-in-vitro-endophytic-fungi-as-plant-growth-promote-of-albizia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74725.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">263</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8030</span> The Effect of Ethylene Glycol on Cryopreserved Bovine Oocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sri%20Wahjuningsih">Sri Wahjuningsih</a>, <a href="https://publications.waset.org/abstracts/search?q=Nur%20Ihsan"> Nur Ihsan</a>, <a href="https://publications.waset.org/abstracts/search?q=Hadiah"> Hadiah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the embryo transfer program, to address the limited production of embryos in vivo, in vitro embryo production has become an alternative approach that is relatively inexpensive. One potential source of embryos that can be developed is to use immature oocytes then conducted in vitro maturation and in vitro fertilization. However, obstacles encountered were oocyte viability mammals have very limited that it cannot be stored for a long time, so we need oocyte cryopreservation. The research was conducted to know the optimal concentration use of ethylene glycol as a cryoprotectant on oocytes freezing.Material use in this research was immature oocytes; taken from abbatoir which was aspirated from follicle with diameter 2-6 mm. Concentration ethylen glycol used were 0,5 M, I M, 1,5 M and 2M. The freezing method used was conventional method combined with a five-step protocol washing oocytes from cryoprotectant after thawing. The result showed that concentration ethylen glycol have the significant effect (P<0.05) on oocytes quality after thawing and in vitro maturation. It was concluded that concentration 1,5 M was the best concentration for freezing oocytes using conventional method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bovine" title="bovine">bovine</a>, <a href="https://publications.waset.org/abstracts/search?q=conventional%20freezing" title=" conventional freezing"> conventional freezing</a>, <a href="https://publications.waset.org/abstracts/search?q=ethylen%20glycol" title=" ethylen glycol"> ethylen glycol</a>, <a href="https://publications.waset.org/abstracts/search?q=oocytes" title=" oocytes"> oocytes</a> </p> <a href="https://publications.waset.org/abstracts/39761/the-effect-of-ethylene-glycol-on-cryopreserved-bovine-oocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39761.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">364</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8029</span> Comparative Study of Antioxidant Activity in in vivo and in vitro Samples of Purple Greater Yam (Dioscorea alata L).</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sakinah%20Abdullah">Sakinah Abdullah</a>, <a href="https://publications.waset.org/abstracts/search?q=Rosna%20Mat%20Taha"> Rosna Mat Taha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Antioxidants are compounds that protect cells against the damaging effects of reactive oxygen species such as singlet oxygen, superoxide, peroxyl radicals, and peroxynitrite which result in oxidative stress leading to cellular damage. Natural antioxidant are in high demand because of their potential in health promotion and disease prevention and their improved safety and consumer acceptability. Plants are rich sources of natural antioxidant. Dioscorea alata L. known as 'ubi badak' in Malaysia were well known for their antioxidant content, but this plant was seasonal. Thus, tissue culture technique was used to mass propagate this plant. In the present work, a comparative study between in vitro (from tissue culture) and in vivo (from intact plant) samples of Dioscorea alata L. for their antioxidant potential by 2,2-diphenil -1- picrylhydrazyl (DPPH) radical scavenging activity method and their total phenolic and flavonoid contents were carried out. All samples had better radical scavenging activity but in vivo samples had the strongest radical scavenging activity compared to in vitro samples. Furthermore, tubers from in vivo samples showed the greatest free radical scavenging effect and comparatively greater phenolic content than in vitro samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dioscorea%20alata" title="Dioscorea alata">Dioscorea alata</a>, <a href="https://publications.waset.org/abstracts/search?q=tissue%20culture" title=" tissue culture"> tissue culture</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vivo" title=" in vivo"> in vivo</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=DPPH" title=" DPPH"> DPPH</a> </p> <a href="https://publications.waset.org/abstracts/31969/comparative-study-of-antioxidant-activity-in-in-vivo-and-in-vitro-samples-of-purple-greater-yam-dioscorea-alata-l" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31969.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">469</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8028</span> Synthesis, Characterization and in vitro DNA Binding and Cleavage Studies of Cu(II)/Zn(II) Dipeptide Complexes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Jamsheera">A. Jamsheera</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Arjmand"> F. Arjmand</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20K.%20Mohapatra"> D. K. Mohapatra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Small molecules binding to specific sites along DNA molecule are considered as potential chemotherapeutic agents. Their role as mediators of key biological functions and their unique intrinsic properties make them particularly attractive therapeutic agents. Keeping in view, novel dipeptide complexes Cu(II)-Val-Pro (1), Zn(II)-Val-Pro (2), Cu(II)-Ala-Pro (3) and Zn(II)-Ala-Pro (4) were synthesized and thoroughly characterized using different spectroscopic techniques including elemental analyses, IR, NMR, ESI–MS and molar conductance measurements. The solution stability study carried out by UV–vis absorption titration over a broad range of pH proved the stability of the complexes in solution. In vitro DNA binding studies of complexes 1–4 carried out employing absorption, fluorescence, circular dichroism and viscometric studies revealed the binding of complexes to DNA via groove binding. UV–vis titrations of 1–4 with mononucleotides of interest viz., 5´-GMP and 5´-TMP were also carried out. The DNA cleavage activity of the complexes 1 and 2 were ascertained by gel electrophoresis assay which revealed that the complexes are good DNA cleavage agents and the cleavage mechanism involved a hydrolytic pathway. Furthermore, in vitro antitumor activity of complex 1 was screened against human cancer cell lines of different histological origin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dipeptide%20Cu%28II%29%20and%20Zn%28II%29%20complexes" title="dipeptide Cu(II) and Zn(II) complexes">dipeptide Cu(II) and Zn(II) complexes</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20binding%20profile" title=" DNA binding profile"> DNA binding profile</a>, <a href="https://publications.waset.org/abstracts/search?q=pBR322%20DNA%20cleavage" title=" pBR322 DNA cleavage"> pBR322 DNA cleavage</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20anticancer%20activity" title=" in vitro anticancer activity"> in vitro anticancer activity</a> </p> <a href="https://publications.waset.org/abstracts/38418/synthesis-characterization-and-in-vitro-dna-binding-and-cleavage-studies-of-cuiiznii-dipeptide-complexes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/38418.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">349</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8027</span> In-Vitro Dextran Synthesis and Characterization of an Intracellular Glucosyltransferase from Leuconostoc Mesenteroides AA1</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Afsheen%20Aman">Afsheen Aman</a>, <a href="https://publications.waset.org/abstracts/search?q=Shah%20Ali%20Ul%20Qader"> Shah Ali Ul Qader</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Dextransucrase [EC 2.4.1.5] is a glucosyltransferase that catalysis the biosynthesis of a natural biopolymer called dextran. It can catalyze the transfer of D-glucopyranosyl residues from sucrose to the main chain of dextran. This unique biopolymer has multiple applications in several industries and the key utilization of dextran lies on its molecular weight and the type of branching. Extracellular dextransucrase from Leuconostoc mesenteroides is most extensively studied and characterized. Limited data is available regarding cell-bound or intracellular dextransucrase and on the characterization of dextran produced by in-vitro reaction of intracellular dextransucrase. L. mesenteroides AA1 is reported to produce extracellular dextransucrase that catalyzes biosynthesis of a high molecular weight dextran with only α-(1→6) linkage. Current study deals with the characterization of an intracellular dextransucrase and in vitro biosynthesis of low molecular weight dextran from L. mesenteroides AA1. Intracellular dextransucrase was extracted from cytoplasm and purified to homogeneity for characterization. Kinetic constants, molecular weight and N-terminal sequence analysis of intracellular dextransucrase reveal unique variation with previously reported extracellular dextransucrase from the same strain. In vitro synthesized biopolymer was characterized using NMR spectroscopic techniques. Intracellular dextransucrase exhibited Vmax and Km values of 130.8 DSU ml-1 hr-1 and 221.3 mM, respectively. Optimum catalytic activity was detected at 35°C in 0.15 M citrate phosphate buffer (pH-5.5) in 05 minutes. Molecular mass of purified intracellular dextransucrase is approximately 220.0 kDa on SDS-PAGE. N-terminal sequence of the intracellular enzyme is: GLPGYFGVN that showed no homology with previously reported sequence for the extracellular dextransucrase. This intracellular dextransucrase is capable of in vitro synthesis of dextran under specific conditions. This intracellular dextransucrase is capable of in vitro synthesis of dextran under specific conditions and this biopolymer can be hydrolyzed into different molecular weight fractions for various applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=characterization" title="characterization">characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=dextran" title=" dextran"> dextran</a>, <a href="https://publications.waset.org/abstracts/search?q=dextransucrase" title=" dextransucrase"> dextransucrase</a>, <a href="https://publications.waset.org/abstracts/search?q=leuconostoc%20mesenteroides" title=" leuconostoc mesenteroides"> leuconostoc mesenteroides</a> </p> <a href="https://publications.waset.org/abstracts/31750/in-vitro-dextran-synthesis-and-characterization-of-an-intracellular-glucosyltransferase-from-leuconostoc-mesenteroides-aa1" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31750.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">396</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20models&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20models&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20models&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20models&page=5">5</a></li> <li class="page-item"><a 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